Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0009987

Physical Location & Seq

Genomic Coordinates Forward (+)

7830862 .. 7832745

Gene Structure 1884 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g09710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGGAATCTCCACCGCCGCGGCCTTCGCTTCAAGAGCTAGACGCGGCGGAGAATATTATTCTCGTCTGGGACTCCACGGCTTCCGAGGAGGCCCGAGAGCGAATGATATTCGACGGCCAGCGCGACGAAATCGATCGCTACTTACAGGCCGTCGATGAAATCCAACGCTCTCTGTCCTCAGCCACGCTTGCTTCAGAGGGCGAACAGTCCAAGCTCAACTCTGCCATCCAGATCGCCATGGCTCGGTTGGAGGACGAGTTCCGCAACATCCTCCTCAACCACACCACTCCCATCGAGCCTGACTCGCTTTCCTCCGCCGATCCGAGCTCGTCAACTCACTCCTGCGTCGCTGATTCGAGCTCCGAGTTCGAGGATTACTCGGCTGAAGACGACGGCGGTTTGACCACACCTAAGGCCTCCTCCGTCTATGCCGGTGACGTCGACGAGCTTCTCCAGCGCGGCGATTCGTCGAACACTAGCAGCTACCGGTCCGCCAGCAGCATCCGCGAGCTCGATCTTATCCCCTCCGATGCGGTATTCGATCTCCGCAACATCGCTGAGCGGATGATTGCCGCCGGCTACCTCCGCGAGTGTATTCAGGTCTACGGCGGCGTCAGGAAGGGGGCGGTGGACTCGAGTTTCCGGAAGCTGGGGATTGAGAAGCTGAGCATCGGAGACGTTCAGAGGCTGCAGTGGGAGCAGCTGGAAACCAAGATCCGACGGTGGATACGCGCCGCCAAGGCCTGCGTGAGGACCGTCTTCGCCTCCGAGAAGAAGCTCTGCGAGCAAATCTTCAATGGCATCGGCACCGCCATTGACGACGCTTGCTTTATGGAGACAGTCAAGGGCCCCGCGATTCAGCTCTTCAACTTCGCCGAGGCCATCAGCATCAGCCGGAGGTCGCCCGAGAAGCTCTTCAAGATTCTCGACTTGCACGATGCTCTGATGGACTTGATGCCAGATATCGAATCGGTTTTCGAATCGAAATCGTCGGAGTCGATTCGGATTCAGGCGGTGGAGATCTTGTCGAGGCTGGCGGAGGCAGCGAGGGGAATTCTATCTGAGTTTGAGAACGCCGTGCTTCGCGAACCGTCTAGGGTTCCCGTCCCCGGCGGAACGATTCATCCATTGACTCGGTATGTGATGAATTACATCAGTTTGATATCGGATTATAAGCAGACTTTGCATGAGCTTATTGTGTCGAAGCCCTCCACGGGGTCGCGGTACTCCGGCGATCCCACGACTCCGGATATGGAGTTCGCCGAGTTGGAGGAGAAGACCCCTCTGGCTCTGCATTTGATTTGGATTATTGTGATTTTGCAATTTAATTTGGATGGCAAGTCTAAGCACTATAAGGATGCTTCAATAGCTCACTTGTTCATGATGAACAATGTTCATTACATTGTTCAAAAGGTGAAAGGGTCTCCGGAATTGCGGGAAATGATTGGAGACGATTACTTGAGGAAGTTGACTGGGAAGTTCCGCCAGGCAGCTACTAGTTACCAGAGAGCTACTTGGGTAGGTGTGTTGTATTGTTTGAGAGACGAAGGGTTACATGTTAGCGGGAGTTTTTCTTCCGGGGTGTCAAAGAGTGCTTTGAGAGAGAGGTTCAAGTCCTTCAATGCTATGTTTGAGGAGGTTCATAGGACCCAAGCTACTTGGTTGATACCGGATACTCAGCTTAGGGAGGAGCTACGGATATCTATATCAGAGAAGTTGATCCCAGCATACAGGTCGTTCCTTGGGCGGTTCAGGAGCCATATAGAAAGTGGGAAGCATCCGGAAAACTACATCAAGTATTCGACCGAGGATTTGGAGATCGCTGTATTGGATTTCTTTGAGGGGTACTCGGTATCCCAGCACTTGAGGAGGAGATCTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0046903 GO:0051179 GO:0051234

KEGG Pathways

Metabolic & Signaling

ko04910 map04910

Pfam Domains

Protein Families

Exo70

Protein Analysis

628

Amino Acids

70.66

Weight (kDa)

5.36

Isoelectric Point (pI)

54.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo70_N	PF20669	13 - 91	2.7e-14	Exocyst complex component Exo70 N-terminal
Exo70_C	PF03081	241 - 608	7.9e-130	Exo70 exocyst complex subunit C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009987)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54090 AT1G72470 AT3G14090
fragaria_vesca	FvH4_6g35110
malus_domestica	MD17G1164500.v1.1
prunus_persica	Prupe.3G038000_v2.0.a1 Prupe.3G038000_v2.0.a1
pyrus_communis	pycom09g09710 pycom17g15760
rosa_chinensis	RchiOBHm_Chr2g0146331
rosa_laevigata	RLG00000020194
rosa_multiflora	Rmu_co8454727.1_g000001 Rmu_sc0004094.1_g000002
rosa_roxburghii	Rroxscaffold_2G00100170
rosa_rugosa	Rorug02G0394100
rosa_samantha	Rh2AG450500 Rh2BG462800 Rh2CG437300 Rh2DG471900 Rh3BG270800
rosa_wichuraiana	Rw2G036770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1173
AatII	GACGTC	1 cut(s)	441
AccB1I	GGYRCC	1 cut(s)	806
AccBSI	CCGCTC	1 cut(s)	562
AccI	GTMKAC	2 cut(s)	441, 603
AccIII	TCCGGA	4 cut(s)	642, 1246, 1426, 1780
AclWI	GGATC	4 cut(s)	314, 709, 1229, 1714
AcoI	YGGCCR	1 cut(s)	115
AcsI	RAATTY	1 cut(s)	1053
AcuI	CTGAAG	2 cut(s)	177, 405
AcyI	GRCGYC	2 cut(s)	438, 612
AfaI	GTAC	2 cut(s)	1226, 1847
AfiI	CCNNNNNNNGG	6 cut(s)	243, 649, 750, 1109, 1214, 1215
AflIII	ACRYGT	1 cut(s)	1555
AgeI	ACCGGT	1 cut(s)	486
AgsI	TTSAA	8 cut(s)	32, 796, 868, 919, 1365, 1409, 1612, 1621
AhlI	ACTAGT	1 cut(s)	1496
AjnI	CCWGG	1 cut(s)	1485
AloI	GAACNNNNNNTCC	6 cut(s)	242, 274, 1108, 1140, 1241, 1273
Alw21I	GWGCWC	3 cut(s)	329, 362, 513
Alw26I	GTCTC	5 cut(s)	669, 830, 1428, 1443, 1536
AlwI	GGATC	4 cut(s)	314, 709, 1229, 1714
AlwNI	CAGNNNCTG	1 cut(s)	688
Ama87I	CYCGRG	3 cut(s)	93, 634, 905
Aor13HI	TCCGGA	4 cut(s)	642, 1246, 1426, 1780
AoxI	GGCC	8 cut(s)	20, 90, 115, 147, 414, 741, 847, 879
ApaI	GGGCCC	1 cut(s)	851
ApeKI	GCWGC	6 cut(s)	480, 498, 688, 700, 1043, 1490
ApoI	RAATTY	1 cut(s)	1053
ArsI	GACNNNNNNTTYG	2 cut(s)	1795, 1827
AsiGI	ACCGGT	1 cut(s)	486
Asp700I	GAANNNNTTC	1 cut(s)	914
AspLEI	GCGC	3 cut(s)	123, 459, 734
AspS9I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1647
AsuC2I	CCSGG	2 cut(s)	1110, 1579
AsuHPI	GGTGA	2 cut(s)	446, 1426
AsuII	TTCGAA	1 cut(s)	978
AvaI	CYCGRG	3 cut(s)	93, 634, 905
AvaII	GGWCC	3 cut(s)	489, 753, 1647
AxyI	CCTNAGG	1 cut(s)	411
BaeGI	GKGCMC	1 cut(s)	851
BanI	GGYRCC	1 cut(s)	806
BanII	GRGCYC	4 cut(s)	329, 362, 513, 851
BbsI	GAAGAC	3 cut(s)	393, 751, 1283
Bbv12I	GWGCWC	3 cut(s)	329, 362, 513
BbvCI	CCTCAGC	1 cut(s)	178
BbvI	GCAGC	6 cut(s)	492, 510, 675, 712, 1055, 1502
BccI	CCATC	5 cut(s)	233, 299, 890, 940, 1328
BceAI	ACGGC	6 cut(s)	93, 130, 134, 409, 622, 1061
BcgI	CGANNNNNNTGC	2 cut(s)	1716, 1750
BciT130I	CCWGG	1 cut(s)	1487
BciVI	GTATCC	3 cut(s)	720, 1666, 1864
BcnI	CCSGG	2 cut(s)	1110, 1579
BcoDI	GTCTC	5 cut(s)	669, 830, 1428, 1443, 1536
BcuI	ACTAGT	1 cut(s)	1496
BfaI	CTAG	4 cut(s)	38, 477, 1095, 1497
BfmI	CTRYAG	1 cut(s)	689
BfuI	GTATCC	3 cut(s)	720, 1666, 1864
BglI	GCCNNNNNGGC	1 cut(s)	740
BglII	AGATCT	2 cut(s)	1020, 1874
BlpI	GCTNAGC	2 cut(s)	558, 665
Bme1390I	CCNGG	3 cut(s)	1110, 1487, 1579
Bme18I	GGWCC	3 cut(s)	489, 753, 1647
BmeT110I	CYCGRG	3 cut(s)	93, 634, 905
BmgT120I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1647
BmiI	GGNNCC	6 cut(s)	808, 849, 850, 1101, 1649, 1757
BmrFI	CCNGG	3 cut(s)	1110, 1487, 1579
BmrI	ACTGGG	1 cut(s)	1482
BmsI	GCATC	9 cut(s)	510, 520, 678, 810, 897, 928, 945, 1348, 1786
BmuI	ACTGGG	1 cut(s)	1482
BpiI	GAAGAC	3 cut(s)	393, 751, 1283
BplI	GAGNNNNNCTC	7 cut(s)	287, 319, 362, 394, 1832, 1864, 1861
BpmI	CTGGAG	1 cut(s)	437
Bpu10I	CCTNAGC	2 cut(s)	178, 1682
Bpu1102I	GCTNAGC	2 cut(s)	558, 665
Bpu14I	TTCGAA	1 cut(s)	978
BpuEI	CTTGAG	1 cut(s)	1480
BpuMI	CCSGG	2 cut(s)	1110, 1579
Bsa29I	ATCGAT	1 cut(s)	132
BsaHI	GRCGYC	2 cut(s)	438, 612
BsaI	GGTCTC	1 cut(s)	1428
BsaWI	WCCGGW	6 cut(s)	486, 642, 1246, 1426, 1669, 1780
BsaXI	ACNNNNNCTCC	4 cut(s)	242, 272, 1010, 1040
Bsc4I	CCNNNNNNNGG	6 cut(s)	243, 649, 750, 1109, 1214, 1215
Bse118I	RCCGGY	3 cut(s)	431, 486, 575
Bse1I	ACTGG	1 cut(s)	1477
Bse21I	CCTNAGG	1 cut(s)	411
BseAI	TCCGGA	4 cut(s)	642, 1246, 1426, 1780
BseBI	CCWGG	1 cut(s)	1487
BseCI	ATCGAT	1 cut(s)	132
BseGI	GGATG	8 cut(s)	225, 267, 501, 570, 1123, 1339, 1363, 1777
BseLI	CCNNNNNNNGG	6 cut(s)	243, 649, 750, 1109, 1214, 1215
BseMII	CTCAG	5 cut(s)	192, 549, 656, 1053, 1691
BseNI	ACTGG	1 cut(s)	1477
BseRI	GAGGAG	7 cut(s)	101, 263, 409, 1286, 1649, 1703, 1882
BseSI	GKGCMC	1 cut(s)	851
BseXI	GCAGC	6 cut(s)	492, 510, 675, 712, 1055, 1502
BseYI	CCCAGC	3 cut(s)	649, 1723, 1857
Bsh1285I	CGRYCG	2 cut(s)	136, 1806
BshFI	GGCC	8 cut(s)	22, 92, 117, 149, 416, 743, 849, 881
BshNI	GGYRCC	1 cut(s)	806
BshTI	ACCGGT	1 cut(s)	486
BshVI	ATCGAT	1 cut(s)	132
BsiEI	CGRYCG	2 cut(s)	136, 1806
BsiHKAI	GWGCWC	3 cut(s)	329, 362, 513
BsiHKCI	CYCGRG	3 cut(s)	93, 634, 905
BslFI	GGGAC	2 cut(s)	83, 1091
BslI	CCNNNNNNNGG	6 cut(s)	243, 649, 750, 1109, 1214, 1215
BsmAI	GTCTC	5 cut(s)	669, 830, 1428, 1443, 1536
BsmBI	CGTCTC	3 cut(s)	669, 1443, 1536
BsmFI	GGGAC	2 cut(s)	83, 1091
BsnI	GGCC	8 cut(s)	22, 92, 117, 149, 416, 743, 849, 881
Bso31I	GGTCTC	1 cut(s)	1428
BsoBI	CYCGRG	3 cut(s)	93, 634, 905
Bsp119I	TTCGAA	1 cut(s)	978
Bsp120I	GGGCCC	1 cut(s)	847
Bsp1286I	GDGCHC	4 cut(s)	329, 362, 513, 851
Bsp13I	TCCGGA	4 cut(s)	642, 1246, 1426, 1780
Bsp1720I	GCTNAGC	2 cut(s)	558, 665
Bsp19I	CCATGG	1 cut(s)	237
Bsp68I	TCGCGA	1 cut(s)	1086
BspANI	GGCC	8 cut(s)	22, 92, 117, 149, 416, 743, 849, 881
BspCNI	CTCAG	5 cut(s)	191, 550, 657, 1054, 1690
BspDI	ATCGAT	1 cut(s)	132
BspEI	TCCGGA	4 cut(s)	642, 1246, 1426, 1780
BspHI	TCATGA	1 cut(s)	1380
BspLI	GGNNCC	6 cut(s)	808, 849, 850, 1101, 1649, 1757
BspMAI	CTGCAG	1 cut(s)	693
BspPI	GGATC	4 cut(s)	314, 709, 1229, 1714
BspQI	GCTCTTC	2 cut(s)	869, 920
BspT104I	TTCGAA	1 cut(s)	978
BspT107I	GGYRCC	1 cut(s)	806
BspTNI	GGTCTC	1 cut(s)	1428
BsrBI	CCGCTC	1 cut(s)	562
BsrFI	RCCGGY	3 cut(s)	431, 486, 575
BsrI	ACTGG	1 cut(s)	1477
BssAI	RCCGGY	3 cut(s)	431, 486, 575
BssNI	GRCGYC	2 cut(s)	438, 612
BssT1I	CCWWGG	3 cut(s)	237, 738, 1741
Bst2UI	CCWGG	1 cut(s)	1487
Bst4CI	ACNGT	5 cut(s)	207, 723, 757, 841, 1092
Bst6I	CTCTTC	2 cut(s)	869, 920
BstACI	GRCGYC	2 cut(s)	438, 612
BstAPI	GCANNNNNTGC	1 cut(s)	1183
BstBI	TTCGAA	1 cut(s)	978
BstC8I	GCNNGC	9 cut(s)	119, 189, 496, 509, 577, 745, 785, 826, 1035
BstDEI	CTNAG	9 cut(s)	178, 411, 558, 665, 1062, 1344, 1677, 1682, 1878
BstDSI	CCRYGG	4 cut(s)	17, 75, 237, 1212
BstENI	CCTNNNNNAGG	1 cut(s)	748
BstF5I	GGATG	8 cut(s)	225, 267, 501, 570, 1123, 1339, 1363, 1777
BstHHI	GCGC	3 cut(s)	123, 459, 734
BstMAI	GTCTC	5 cut(s)	669, 830, 1428, 1443, 1536
BstMCI	CGRYCG	2 cut(s)	136, 1806
BstNI	CCWGG	1 cut(s)	1487
BstNSI	RCATGY	1 cut(s)	1559
BstSCI	CCNGG	3 cut(s)	1108, 1485, 1577
BstSFI	CTRYAG	1 cut(s)	689
BstSLI	GKGCMC	1 cut(s)	851
BstV1I	GCAGC	6 cut(s)	492, 510, 675, 712, 1055, 1502
BstV2I	GAAGAC	3 cut(s)	393, 751, 1283
BstX2I	RGATCY	3 cut(s)	714, 1020, 1874
BstYI	RGATCY	3 cut(s)	714, 1020, 1874
Bsu15I	ATCGAT	1 cut(s)	132
Bsu36I	CCTNAGG	1 cut(s)	411
BsuI	GTATCC	3 cut(s)	720, 1666, 1864
BsuRI	GGCC	8 cut(s)	22, 92, 117, 149, 416, 743, 849, 881
BsuTUI	ATCGAT	1 cut(s)	132
BtgI	CCRYGG	4 cut(s)	17, 75, 237, 1212
BtgZI	GCGATG	1 cut(s)	538
BtsCI	GGATG	8 cut(s)	225, 267, 501, 570, 1123, 1339, 1363, 1777
BtsI	GCAGTG	1 cut(s)	698
BtsIMutI	CAGTG	1 cut(s)	698
BtuMI	TCGCGA	1 cut(s)	1086
Cac8I	GCNNGC	9 cut(s)	119, 189, 496, 509, 577, 745, 785, 826, 1035
CaiI	CAGNNNCTG	1 cut(s)	688
CciI	TCATGA	1 cut(s)	1380
CfoI	GCGC	3 cut(s)	123, 459, 734
Cfr10I	RCCGGY	3 cut(s)	431, 486, 575
Cfr13I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1647
Cfr42I	CCGCGG	1 cut(s)	20
ClaI	ATCGAT	1 cut(s)	132
CpoI	CGGWCCG	1 cut(s)	489
CseI	GACGC	4 cut(s)	50, 334, 601, 830
Csp6I	GTAC	2 cut(s)	1225, 1846
CspAI	ACCGGT	1 cut(s)	486
CspI	CGGWCCG	1 cut(s)	489
CviAII	CATG	4 cut(s)	238, 1187, 1381, 1556
CviQI	GTAC	2 cut(s)	1225, 1846
DdeI	CTNAG	9 cut(s)	178, 411, 558, 665, 1062, 1344, 1677, 1682, 1878
EaeI	YGGCCR	1 cut(s)	115
Eam1104I	CTCTTC	2 cut(s)	869, 920
EarI	CTCTTC	2 cut(s)	869, 920
EciI	GGCGGA	6 cut(s)	62, 304, 481, 1052, 1128, 1472
Ecl136II	GAGCTC	3 cut(s)	327, 360, 511
Eco130I	CCWWGG	3 cut(s)	237, 738, 1741
Eco147I	AGGCCT	2 cut(s)	416, 743
Eco24I	GRGCYC	4 cut(s)	329, 362, 513, 851
Eco31I	GGTCTC	1 cut(s)	1428
Eco32I	GATATC	3 cut(s)	964, 1164, 1701
Eco47I	GGWCC	3 cut(s)	489, 753, 1647
Eco53kI	GAGCTC	3 cut(s)	327, 360, 511
Eco57I	CTGAAG	2 cut(s)	177, 405
Eco81I	CCTNAGG	1 cut(s)	411
Eco88I	CYCGRG	3 cut(s)	93, 634, 905
EcoICRI	GAGCTC	3 cut(s)	327, 360, 511
EcoNI	CCTNNNNNAGG	1 cut(s)	748
EcoO109I	RGGNCCY	3 cut(s)	847, 848, 1647
EcoRI	GAATTC	1 cut(s)	1053
EcoRII	CCWGG	1 cut(s)	1485
EcoRV	GATATC	3 cut(s)	964, 1164, 1701
EcoT14I	CCWWGG	3 cut(s)	237, 738, 1741
EcoT38I	GRGCYC	4 cut(s)	329, 362, 513, 851
ErhI	CCWWGG	3 cut(s)	237, 738, 1741
Esp3I	CGTCTC	3 cut(s)	669, 1443, 1536
FaeI	CATG	4 cut(s)	241, 1190, 1384, 1559
FaqI	GGGAC	2 cut(s)	83, 1091
FatI	CATG	4 cut(s)	237, 1186, 1380, 1555
FauI	CCCGC	3 cut(s)	859, 1428, 1556
FblI	GTMKAC	2 cut(s)	441, 603
FokI	GGATG	8 cut(s)	212, 254, 488, 577, 1110, 1346, 1370, 1764
FriOI	GRGCYC	4 cut(s)	329, 362, 513, 851
FspBI	CTAG	4 cut(s)	38, 477, 1095, 1497
GlaI	GCGC	3 cut(s)	122, 458, 733
GsaI	CCCAGC	3 cut(s)	653, 1727, 1861
GsuI	CTGGAG	1 cut(s)	437
HaeIII	GGCC	8 cut(s)	22, 92, 117, 149, 416, 743, 849, 881
HgaI	GACGC	4 cut(s)	50, 334, 601, 830
HhaI	GCGC	3 cut(s)	123, 459, 734
Hin1I	GRCGYC	2 cut(s)	438, 612
Hin1II	CATG	4 cut(s)	241, 1190, 1384, 1559
Hin6I	GCGC	3 cut(s)	121, 457, 732
HinP1I	GCGC	3 cut(s)	121, 457, 732
HincII	GTYRAC	3 cut(s)	333, 442, 1470
HindII	GTYRAC	3 cut(s)	333, 442, 1470
HphI	GGTGA	2 cut(s)	446, 1426
Hpy166II	GTNNAC	5 cut(s)	333, 442, 604, 631, 1470
Hpy8I	GTNNAC	5 cut(s)	333, 442, 604, 631, 1470
HpyAV	CCTTC	4 cut(s)	33, 613, 1541, 1627
HpyCH4III	ACNGT	5 cut(s)	207, 723, 757, 841, 1092
HpyCH4IV	ACGT	2 cut(s)	438, 678
HpyCH4V	TGCA	5 cut(s)	691, 934, 1186, 1294, 1321
HpyF3I	CTNAG	9 cut(s)	178, 411, 558, 665, 1062, 1344, 1677, 1682, 1878
HpySE526I	ACGT	2 cut(s)	438, 678
Hsp92I	GRCGYC	2 cut(s)	438, 612
Hsp92II	CATG	4 cut(s)	241, 1190, 1384, 1559
HspAI	GCGC	3 cut(s)	121, 457, 732
Kpn2I	TCCGGA	4 cut(s)	642, 1246, 1426, 1780
KroI	GCCGGC	1 cut(s)	575
KroNI	GCCGGC	1 cut(s)	577
KspI	CCGCGG	1 cut(s)	20
LguI	GCTCTTC	2 cut(s)	869, 920
LmnI	GCTCC	4 cut(s)	365, 697, 1690, 1755
Lsp1109I	GCAGC	6 cut(s)	492, 510, 675, 712, 1055, 1502
LweI	GCATC	9 cut(s)	510, 520, 678, 810, 897, 928, 945, 1348, 1786
MaeI	CTAG	4 cut(s)	38, 477, 1095, 1497
MaeII	ACGT	2 cut(s)	438, 678
MaeIII	GTNAC	3 cut(s)	434, 1499, 1551
MbiI	CCGCTC	1 cut(s)	562
MboII	GAAGA	8 cut(s)	398, 751, 784, 784, 856, 907, 1288, 1566
MflI	RGATCY	3 cut(s)	714, 1020, 1874
MhlI	GDGCHC	4 cut(s)	329, 362, 513, 851
MluCI	AATT	5 cut(s)	1053, 1147, 1322, 1327, 1430
MlyI	GAGTC	6 cut(s)	65, 296, 626, 1004, 1126, 1237
MmeI	TCCRAC	5 cut(s)	187, 228, 742, 972, 1248
MroI	TCCGGA	4 cut(s)	642, 1246, 1426, 1780
MroNI	GCCGGC	1 cut(s)	575
MroXI	GAANNNNTTC	1 cut(s)	914
MseI	TTAA	1 cut(s)	1326
MspA1I	CMGCKG	2 cut(s)	19, 703
MspR9I	CCNGG	3 cut(s)	1110, 1487, 1579
MvaI	CCWGG	1 cut(s)	1487
NaeI	GCCGGC	1 cut(s)	577
NciI	CCSGG	2 cut(s)	1110, 1579
NcoI	CCATGG	1 cut(s)	237
NgoMIV	GCCGGC	1 cut(s)	575
NlaIII	CATG	4 cut(s)	241, 1190, 1384, 1559
NlaIV	GGNNCC	6 cut(s)	808, 849, 850, 1101, 1649, 1757
NmeAIII	GCCGAG	3 cut(s)	359, 901, 1288
NmuCI	GTSAC	1 cut(s)	434
NruI	TCGCGA	1 cut(s)	1086
NspI	RCATGY	1 cut(s)	1559
NspV	TTCGAA	1 cut(s)	978
PaeR7I	CTCGAG	1 cut(s)	634
PagI	TCATGA	1 cut(s)	1380
PceI	AGGCCT	2 cut(s)	416, 743
PciI	ACATGT	1 cut(s)	1555
PciSI	GCTCTTC	2 cut(s)	869, 920
PcsI	WCGNNNNNNNCGW	3 cut(s)	120, 933, 995
PdiI	GCCGGC	1 cut(s)	577
PdmI	GAANNNNTTC	1 cut(s)	914
PinAI	ACCGGT	1 cut(s)	486
Ple19I	CGATCG	1 cut(s)	136
PleI	GAGTC	6 cut(s)	65, 296, 626, 1003, 1126, 1237
PpsI	GAGTC	6 cut(s)	65, 296, 626, 1003, 1126, 1237
PpuMI	RGGWCCY	1 cut(s)	1647
PscI	ACATGT	1 cut(s)	1555
PsiI	TTATAA	1 cut(s)	1173
Psp124BI	GAGCTC	3 cut(s)	329, 362, 513
Psp5II	RGGWCCY	1 cut(s)	1647
Psp6I	CCWGG	1 cut(s)	1485
PspFI	CCCAGC	3 cut(s)	649, 1723, 1857
PspGI	CCWGG	1 cut(s)	1485
PspN4I	GGNNCC	6 cut(s)	808, 849, 850, 1101, 1649, 1757
PspOMI	GGGCCC	1 cut(s)	847
PspPI	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1647
PspPPI	RGGWCCY	1 cut(s)	1647
PspXI	VCTCGAGB	1 cut(s)	634
PstI	CTGCAG	1 cut(s)	693
PstNI	CAGNNNCTG	1 cut(s)	688
PsuI	RGATCY	3 cut(s)	714, 1020, 1874
PvuI	CGATCG	1 cut(s)	136
PvuII	CAGCTG	1 cut(s)	703
RruI	TCGCGA	1 cut(s)	1086
RsaI	GTAC	2 cut(s)	1226, 1847
RsaNI	GTAC	2 cut(s)	1225, 1846
Rsr2I	CGGWCCG	1 cut(s)	489
RsrII	CGGWCCG	1 cut(s)	489
SacI	GAGCTC	3 cut(s)	329, 362, 513
SacII	CCGCGG	1 cut(s)	20
SalI	GTCGAC	1 cut(s)	440
SapI	GCTCTTC	2 cut(s)	869, 920
SaqAI	TTAA	1 cut(s)	1326
Sau96I	GGNCC	6 cut(s)	91, 489, 753, 847, 848, 1647
SchI	GAGTC	6 cut(s)	65, 296, 626, 1004, 1126, 1237
ScrFI	CCNGG	3 cut(s)	1110, 1487, 1579
SduI	GDGCHC	4 cut(s)	329, 362, 513, 851
SfaNI	GCATC	9 cut(s)	510, 520, 678, 810, 897, 928, 945, 1348, 1786
SfcI	CTRYAG	1 cut(s)	689
Sfr274I	CTCGAG	1 cut(s)	634
Sfr303I	CCGCGG	1 cut(s)	20
SfuI	TTCGAA	1 cut(s)	978
SgrBI	CCGCGG	1 cut(s)	20
SgrDI	CGTCGACG	1 cut(s)	440
SinI	GGWCC	3 cut(s)	489, 753, 1647
SlaI	CTCGAG	1 cut(s)	634
SmlI	CTYRAG	3 cut(s)	634, 1459, 1864
SmoI	CTYRAG	3 cut(s)	634, 1459, 1864
SpeI	ACTAGT	1 cut(s)	1496
Sse9I	AATT	5 cut(s)	1053, 1147, 1322, 1327, 1430
SseBI	AGGCCT	2 cut(s)	416, 743
SspI	AATATT	1 cut(s)	55
SspMI	CTAG	4 cut(s)	38, 477, 1095, 1497
SstI	GAGCTC	3 cut(s)	329, 362, 513
StuI	AGGCCT	2 cut(s)	416, 743
StyD4I	CCNGG	3 cut(s)	1108, 1485, 1577
StyI	CCWWGG	3 cut(s)	237, 738, 1741
TaaI	ACNGT	5 cut(s)	207, 723, 757, 841, 1092
TaiI	ACGT	2 cut(s)	441, 681
TaqII	GACCGA	1 cut(s)	1820
TasI	AATT	5 cut(s)	1053, 1147, 1322, 1327, 1430
TauI	GCSGC	7 cut(s)	19, 22, 47, 462, 575, 612, 737
Tru1I	TTAA	1 cut(s)	1326
Tru9I	TTAA	1 cut(s)	1326
TscAI	CASTG	1 cut(s)	698
TseFI	GTSAC	1 cut(s)	434
TseI	GCWGC	6 cut(s)	480, 498, 688, 700, 1043, 1490
Tsp45I	GTSAC	1 cut(s)	434
TspDTI	ATGAA	7 cut(s)	171, 1112, 1160, 1369, 1385, 1400, 1631
TspGWI	ACGGA	2 cut(s)	412, 1711
TspRI	CASTG	1 cut(s)	698
VpaK11BI	GGWCC	3 cut(s)	489, 753, 1647
XagI	CCTNNNNNAGG	1 cut(s)	748
XapI	RAATTY	1 cut(s)	1053
XceI	RCATGY	1 cut(s)	1559
XcmI	CCANNNNNNNNNTGG	2 cut(s)	244, 1766
XhoI	CTCGAG	1 cut(s)	634
XmiI	GTMKAC	2 cut(s)	441, 603
XmnI	GAANNNNTTC	1 cut(s)	914
XspI	CTAG	4 cut(s)	38, 477, 1095, 1497
ZraI	GACGTC	1 cut(s)	439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.