Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0014956

Physical Location & Seq

Genomic Coordinates Reverse (-)

24235709 .. 24237096

Gene Structure 1388 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1200700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 942 bp

ATGGGTTCTGAAGTTGAGCAAAAGCTTCCTGTCCTAGATTTCTCCAAGGAAGCTCTGAAGCCAGGGACCAACTCATGGCTCTTGGCATGCAAAGATGTTCAACGAGCACTTGAAGAGTTTGGATGTTTTGTAGTGTTGTATGACAAAATCTCTGATGAATTTCGCAATGCTTTTGTTGGTTCCCTTGAAGAACTATTTGATCTCCCTACCGAGACCAAAATGACAAACAAATATGAAAAGCCCTTAAATGGCTATGTGGGACAAATTCCCAAACTCCCTCTCCATGAAAGCTTGGGCATCGACTGTGCAACCAATTTTGAGGAGACTAAAAAATTCACAAAGCTCATGTGGCCTGCTGGAAATGATAAATTCTGTGAAAGGGCATATGCGTTTGCAAAAGTGGCGGAGGAGTTGGATCAAATGGTGGCTAGAATGATATTTGAGAGCTATGGTGTGGAGAAGTATTATGACTCGTATGTTGGATCGATCTCTTACCTTCTTCGGATCTTGCAAAATAGATCACCCAAAGAAAATGAGCACAATCTTGGCTTCGTTGCTCATACTGACAAGAGCTTCACAACCGTACTTTATCAGAATAATCAAGTCAATGCTCTGGAGGTTGAAACAAGGAACCACGAATGGATTAAAGTTGAGTTTCCACCTTCATCCTTCATAGTCATGGCGGGTGATGCCCTAATGGCATGGAGCAATGACAGGATATTATCTCCTAATCATCGAGTTATTATGAGTGGGAATGAAACAAGATACTCGTTGGCGCAGTTTGCATTTAGCGATGGCGAGATACATGTACCCGAAGAGCTTGGCGATGAAGAACGCCCATTACGCTACAAGTCATTTGATCATCCTGGACTACTACGTTTCTTTCGCTCTCAAGATCGTTATATTTCAAACAGTGCTATAAAAGCCTACTGTGGTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0045544 GO:0046394 GO:0048367 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0090567 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

314

Amino Acids

35.93

Weight (kDa)

5.25

Isoelectric Point (pI)

36.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	9 - 87	1.5e-09	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	176 - 262	4e-20	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014956)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52790
fragaria_vesca	FvH4_6g30751 FvH4_6g30751
malus_domestica	MD17G1200700.v1.1
prunus_persica	Prupe.3G076300_v2.0.a1 Prupe.4G141200_v2.0.a1
pyrus_communis	pycom17g20420 pycom17g20470
rosa_chinensis	RchiOBHm_Chr2g0137171
rosa_laevigata	RLG00000019630
rosa_multiflora	Rmu_sc0000894.1_g000049
rosa_roxburghii	Rroxscaffold_2G00107420
rosa_rugosa	Rorug02G0339500
rosa_samantha	Rh2CG375200 Rh2DG410700
rosa_wichuraiana	Rw2G031790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	75
AciI	CCGC	2 cut(s)	404, 683
AclWI	GGATC	3 cut(s)	423, 490, 512
AcsI	RAATTY	4 cut(s)	158, 264, 332, 368
AcuI	CTGAAG	2 cut(s)	30, 77
AfaI	GTAC	2 cut(s)	585, 810
AfiI	CCNNNNNNNGG	2 cut(s)	75, 248
AflIII	ACRYGT	1 cut(s)	805
AgsI	TTSAA	5 cut(s)	101, 113, 188, 623, 909
AjnI	CCWGG	2 cut(s)	61, 865
AluBI	AGCT	7 cut(s)	25, 53, 291, 343, 447, 573, 820
AluI	AGCT	7 cut(s)	25, 53, 291, 343, 447, 573, 820
Alw21I	GWGCWC	2 cut(s)	109, 540
Alw26I	GTCTC	2 cut(s)	206, 317
AlwI	GGATC	3 cut(s)	423, 490, 512
AoxI	GGCC	1 cut(s)	350
ApoI	RAATTY	4 cut(s)	158, 264, 332, 368
AspLEI	GCGC	1 cut(s)	778
AspS9I	GGNCC	1 cut(s)	66
AsuHPI	GGTGA	2 cut(s)	513, 698
AvaII	GGWCC	1 cut(s)	66
Bbv12I	GWGCWC	2 cut(s)	109, 540
BccI	CCATC	1 cut(s)	788
BciT130I	CCWGG	2 cut(s)	63, 867
BclI	TGATCA	1 cut(s)	859
BcoDI	GTCTC	2 cut(s)	206, 317
BfaI	CTAG	2 cut(s)	35, 429
BglI	GCCNNNNNGGC	1 cut(s)	698
Bme1390I	CCNGG	2 cut(s)	63, 867
Bme18I	GGWCC	1 cut(s)	66
BmgT120I	GGNCC	1 cut(s)	66
BmiI	GGNNCC	3 cut(s)	67, 181, 632
BmrFI	CCNGG	2 cut(s)	63, 867
BmsI	GCATC	2 cut(s)	306, 679
BpmI	CTGGAG	1 cut(s)	635
BpuEI	CTTGAG	1 cut(s)	876
Bsa29I	ATCGAT	1 cut(s)	485
BsaI	GGTCTC	1 cut(s)	206
BsaJI	CCNNGG	2 cut(s)	45, 62
BsaXI	ACNNNNNCTCC	2 cut(s)	264, 294
Bsc4I	CCNNNNNNNGG	2 cut(s)	75, 248
Bse3DI	GCAATG	2 cut(s)	172, 715
BseBI	CCWGG	2 cut(s)	63, 867
BseCI	ATCGAT	1 cut(s)	485
BseDI	CCNNGG	2 cut(s)	45, 62
BseGI	GGATG	3 cut(s)	128, 665, 862
BseLI	CCNNNNNNNGG	2 cut(s)	75, 248
BseMI	GCAATG	2 cut(s)	172, 715
BseRI	GAGGAG	2 cut(s)	335, 422
BshFI	GGCC	1 cut(s)	352
BshVI	ATCGAT	1 cut(s)	485
BsiHKAI	GWGCWC	2 cut(s)	109, 540
BslFI	GGGAC	2 cut(s)	79, 273
BslI	CCNNNNNNNGG	2 cut(s)	75, 248
BsmAI	GTCTC	2 cut(s)	206, 317
BsmFI	GGGAC	2 cut(s)	79, 273
BsnI	GGCC	1 cut(s)	352
Bso31I	GGTCTC	1 cut(s)	206
Bsp1286I	GDGCHC	2 cut(s)	109, 540
Bsp143I	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
BspACI	CCGC	2 cut(s)	404, 683
BspANI	GGCC	1 cut(s)	352
BspDI	ATCGAT	1 cut(s)	485
BspLI	GGNNCC	3 cut(s)	67, 181, 632
BspPI	GGATC	3 cut(s)	423, 490, 512
BspQI	GCTCTTC	1 cut(s)	810
BspTNI	GGTCTC	1 cut(s)	206
BsrDI	GCAATG	2 cut(s)	172, 715
BssECI	CCNNGG	2 cut(s)	45, 62
BssMI	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
BssT1I	CCWWGG	1 cut(s)	45
Bst2UI	CCWGG	2 cut(s)	63, 867
Bst4CI	ACNGT	4 cut(s)	305, 583, 914, 932
Bst6I	CTCTTC	2 cut(s)	108, 810
BstC8I	GCNNGC	2 cut(s)	88, 354
BstF5I	GGATG	3 cut(s)	128, 665, 862
BstHHI	GCGC	1 cut(s)	778
BstKTI	GATC	8 cut(s)	202, 418, 485, 489, 507, 521, 862, 898
BstMAI	GTCTC	2 cut(s)	206, 317
BstMBI	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
BstMWI	GCNNNNNNNGC	7 cut(s)	349, 401, 689, 698, 782, 843, 923
BstNI	CCWGG	2 cut(s)	63, 867
BstNSI	RCATGY	2 cut(s)	90, 809
BstSCI	CCNGG	2 cut(s)	61, 865
BstX2I	RGATCY	1 cut(s)	504
BstYI	RGATCY	1 cut(s)	504
Bsu15I	ATCGAT	1 cut(s)	485
BsuRI	GGCC	1 cut(s)	352
BsuTUI	ATCGAT	1 cut(s)	485
BtgZI	GCGATG	2 cut(s)	807, 840
BtsCI	GGATG	3 cut(s)	128, 665, 862
BtsIMutI	CAGTG	1 cut(s)	919
Cac8I	GCNNGC	2 cut(s)	88, 354
CfoI	GCGC	1 cut(s)	778
Cfr13I	GGNCC	1 cut(s)	66
ClaI	ATCGAT	1 cut(s)	485
Csp6I	GTAC	2 cut(s)	584, 809
CviAII	CATG	7 cut(s)	75, 87, 284, 346, 679, 702, 806
CviQI	GTAC	2 cut(s)	584, 809
DpnI	GATC	8 cut(s)	201, 417, 484, 488, 506, 520, 861, 897
DpnII	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
Eam1104I	CTCTTC	2 cut(s)	108, 810
EarI	CTCTTC	2 cut(s)	108, 810
EciI	GGCGGA	1 cut(s)	419
Eco130I	CCWWGG	1 cut(s)	45
Eco31I	GGTCTC	1 cut(s)	206
Eco47I	GGWCC	1 cut(s)	66
Eco57I	CTGAAG	2 cut(s)	30, 77
EcoRII	CCWGG	2 cut(s)	61, 865
EcoT14I	CCWWGG	1 cut(s)	45
ErhI	CCWWGG	1 cut(s)	45
FaeI	CATG	7 cut(s)	78, 90, 287, 349, 682, 705, 809
FaqI	GGGAC	2 cut(s)	79, 273
FatI	CATG	7 cut(s)	74, 86, 283, 345, 678, 701, 805
FauI	CCCGC	1 cut(s)	676
FauNDI	CATATG	1 cut(s)	385
FbaI	TGATCA	1 cut(s)	859
FokI	GGATG	3 cut(s)	135, 652, 849
FspBI	CTAG	2 cut(s)	35, 429
GlaI	GCGC	1 cut(s)	777
GsuI	CTGGAG	1 cut(s)	635
HaeIII	GGCC	1 cut(s)	352
HhaI	GCGC	1 cut(s)	778
Hin1II	CATG	7 cut(s)	78, 90, 287, 349, 682, 705, 809
Hin6I	GCGC	1 cut(s)	776
HinP1I	GCGC	1 cut(s)	776
HindIII	AAGCTT	2 cut(s)	23, 289
HinfI	GANTC	1 cut(s)	470
HphI	GGTGA	2 cut(s)	513, 698
Hpy188I	TCNGA	5 cut(s)	10, 57, 154, 504, 594
Hpy188III	TCNNGA	2 cut(s)	614, 893
HpyAV	CCTTC	3 cut(s)	506, 672, 679
HpyCH4III	ACNGT	4 cut(s)	305, 583, 914, 932
HpyCH4IV	ACGT	1 cut(s)	877
HpyCH4V	TGCA	5 cut(s)	90, 308, 395, 511, 785
HpyF10VI	GCNNNNNNNGC	7 cut(s)	349, 401, 689, 698, 782, 843, 923
HpySE526I	ACGT	1 cut(s)	877
Hsp92II	CATG	7 cut(s)	78, 90, 287, 349, 682, 705, 809
HspAI	GCGC	1 cut(s)	776
Ksp22I	TGATCA	1 cut(s)	859
Kzo9I	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
LguI	GCTCTTC	1 cut(s)	810
LmnI	GCTCC	1 cut(s)	705
LpnPI	CCDG	9 cut(s)	42, 48, 75, 342, 366, 599, 700, 852, 879
LweI	GCATC	2 cut(s)	306, 679
MaeI	CTAG	2 cut(s)	35, 429
MaeII	ACGT	1 cut(s)	877
MalI	GATC	8 cut(s)	201, 417, 484, 488, 506, 520, 861, 897
MboI	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
MboII	GAAGA	5 cut(s)	125, 200, 491, 827, 842
MflI	RGATCY	1 cut(s)	504
MhlI	GDGCHC	2 cut(s)	109, 540
MluCI	AATT	5 cut(s)	158, 264, 313, 332, 368
MlyI	GAGTC	1 cut(s)	464
MmeI	TCCRAC	2 cut(s)	393, 460
MnlI	CCTC	4 cut(s)	288, 313, 400, 610
MseI	TTAA	2 cut(s)	245, 645
MslI	CAYNNNNRTG	1 cut(s)	677
MspR9I	CCNGG	2 cut(s)	63, 867
MvaI	CCWGG	2 cut(s)	63, 867
MwoI	GCNNNNNNNGC	7 cut(s)	349, 401, 689, 698, 782, 843, 923
NdeI	CATATG	1 cut(s)	385
NdeII	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
NlaIII	CATG	7 cut(s)	78, 90, 287, 349, 682, 705, 809
NlaIV	GGNNCC	3 cut(s)	67, 181, 632
NspI	RCATGY	2 cut(s)	90, 809
PaeI	GCATGC	1 cut(s)	90
PciI	ACATGT	1 cut(s)	805
PciSI	GCTCTTC	1 cut(s)	810
PflMI	CCANNNNNTGG	1 cut(s)	75
PfoI	TCCNGGA	1 cut(s)	865
PleI	GAGTC	1 cut(s)	464
PpsI	GAGTC	1 cut(s)	464
PscI	ACATGT	1 cut(s)	805
Psp6I	CCWGG	2 cut(s)	61, 865
PspGI	CCWGG	2 cut(s)	61, 865
PspN4I	GGNNCC	3 cut(s)	67, 181, 632
PspPI	GGNCC	1 cut(s)	66
PsrI	GAACNNNNNNTAC	2 cut(s)	825, 857
PsuI	RGATCY	1 cut(s)	504
RsaI	GTAC	2 cut(s)	585, 810
RsaNI	GTAC	2 cut(s)	584, 809
RseI	CAYNNNNRTG	1 cut(s)	677
SapI	GCTCTTC	1 cut(s)	810
SaqAI	TTAA	2 cut(s)	245, 645
Sau3AI	GATC	8 cut(s)	199, 415, 482, 486, 504, 518, 859, 895
Sau96I	GGNCC	1 cut(s)	66
SchI	GAGTC	1 cut(s)	464
ScrFI	CCNGG	2 cut(s)	63, 867
SduI	GDGCHC	2 cut(s)	109, 540
SfaNI	GCATC	2 cut(s)	306, 679
SinI	GGWCC	1 cut(s)	66
SmiMI	CAYNNNNRTG	1 cut(s)	677
SmlI	CTYRAG	1 cut(s)	891
SmoI	CTYRAG	1 cut(s)	891
SphI	GCATGC	1 cut(s)	90
Sse9I	AATT	5 cut(s)	158, 264, 313, 332, 368
SsiI	CCGC	2 cut(s)	404, 683
SspMI	CTAG	2 cut(s)	35, 429
StyD4I	CCNGG	2 cut(s)	61, 865
StyI	CCWWGG	1 cut(s)	45
TaaI	ACNGT	4 cut(s)	305, 583, 914, 932
TaiI	ACGT	1 cut(s)	880
TaqI	TCGA	3 cut(s)	300, 485, 736
TasI	AATT	5 cut(s)	158, 264, 313, 332, 368
Tru1I	TTAA	2 cut(s)	245, 645
Tru9I	TTAA	2 cut(s)	245, 645
TscAI	CASTG	1 cut(s)	919
TspDTI	ATGAA	7 cut(s)	171, 249, 300, 654, 661, 771, 843
TspRI	CASTG	1 cut(s)	919
Van91I	CCANNNNNTGG	1 cut(s)	75
VpaK11BI	GGWCC	1 cut(s)	66
XapI	RAATTY	4 cut(s)	158, 264, 332, 368
XceI	RCATGY	2 cut(s)	90, 809
XspI	CTAG	2 cut(s)	35, 429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.