Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0015661

Physical Location & Seq

Genomic Coordinates Reverse (-)

28245403 .. 28250477

Gene Structure 5075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1233800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGCAAAGAAACTTGCAAAAATACTCCTACTCCTAGCCATCTTGTTACTTGAACAAGCCGGCCCCGGCCAAAGTCGATCGATTACCAATGATGACGTCCCTTTGAGTATGGTCACTGATGGAGGAAACCAATTACTTCTCCAAAACCAAAATGTTTGGAAACCGTTTTTCTCTGGGCTGATGGACTCTTTGTTCTCATCAGATAGCAGCTGTGAACAGACATATGGGTTCTTGCCATGCACGTCAACAGTTCTGGGAAATATCTTCCTCGTTCTTGTGTATGGATATATGATGTTCCTTTCAGCCAAGCTCTTATCTAATGGAAGTGAGATTCTGCTGCAGATCTTAGGGCCTGGCATCGTCGGCGGTCTATTTCTTCCTCTTTTAGGCTCTCTACCTGATGCCACCATTATTCTAGCATCTGGACTTTCTGGAGACACAGAAACGGCTCAAAGTCAGGTCTCAGTTGGAATGGGGTTGATGGCTGGATCAAATGTCATGCTTCTGACAATTCTATGGGGCACCTGTCTCATCGTTGGCAAATGTGACATTGAAAATTCAATTGCAGTTGATCAAAAAGATGAAAAACGATTTAGCTTAACTGGATCTGGAGTGAGCACAGATATTTGGACAAGCTATTCTGCACGGATCATGATTATCTCTTTGATCCCGTTTGTGATTGTCCAGTTACCCCAAGTTTTCCACGCAACTTCAGATAGTCGCGTAGCAATTCTGATTTCACTCGCTGTCTCCATCTCTTTCGTGATCGCATATAGCTTTTATCAGGTATTTCAACCATGGATTCAGAAGAGAAAACTTGCATATTCAAAGCATAAGCATGTGATGTCAGAAATTCTAAAACAATTGAAGACAAATGCTCTCGGAAGACTTCTTACAAATGATGGTGACCCTAACAAAGTTATTATACAAAAGTTATTCAAGACACTTGATCAGGATTCGGATGGTTACCTAAATACCTCAGACTTAAGATCTCTGATAATTGGAATCCAGTTTGACGATATAGACATAAATATAGATGAAGCCATTATACAAGTGATGAGAGATTTTGATACATCTCATGACTCAAAGATTGACGAGGACGAATTTTTCAGAGGAATCTCAAGATGGCTTAACAAGGCTAAGCGTGAGGCAATAAAGGACCGTGGAAAGATTCCACGGTCGATGAAACTTTTAGAGGAATATGATCAGAAAACAAAGAAGGAATATGATCAGTTTGGGGATCAAATTGATGAGGTTGTTGAGGATGTAAAAAATGCTAAGTGGCATGCCTCCAAAGCAGTAGTGTTGTTGTTTCTGGGAACTATAGTCGCTGCTGCAGTTGCTGATCCCCTTGTTGATGCTGTTGACAGCTTCTCAACAGCTACGAGTATCCCTTCGTTCTTCATCTCATTTGTTGTTCTACCTTTTGCTAGCTCCAGTGAGATAGTAACCACTCTAATTTTTATCAGCCGGAAAAAGCAAAGAACTGCATCTTTGGCATACTCTGAGATATACGGGTCAGTGACAATGAGTAACATACTTTCTCTAGCAGTATTCTTGGGTCTGGTTTACGTTCGAAACTTGACATGGAGCTTCTCAGCTGAAGTTCTCGTCATTTTAATTGTTTGCATTCTGATGGGAGCGATTGCCAGTTTCCGCACCACCTTCCCTCTTTGGATGAGTTCTGTGGCTATACTGCTTTATCCACTTTCTCTGTTGTTGGTTTATATTCTTGATTACATATGTGGGTGGTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EF-hand_7 Na_Ca_ex

Protein Analysis

586

Amino Acids

64.56

Weight (kDa)

5.34

Isoelectric Point (pI)

39.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_Ca_ex	PF01699	88 - 265	6.4e-09	Sodium/calcium exchanger protein
EF-hand_7	PF13499	309 - 372	4.3e-11	EF-hand domain pair
Na_Ca_ex	PF01699	433 - 576	1.4e-11	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015661)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g26990 FvH4_6g26990 FvH4_6g26990 FvH4_6g26990
malus_domestica	MD17G1233800.v1.1
prunus_persica	Prupe.3G125200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0128851
rosa_laevigata	RLG00000019033
rosa_multiflora	Rmu_sc0001643.1_g000033
rosa_roxburghii	Rroxscaffold_2G00115170
rosa_rugosa	Rorug02G0281800 Rorug02G0281800
rosa_samantha	Rh2AG334300 Rh2CG321200 Rh2DG359000
rosa_wichuraiana	Rw2G027040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	99
AccB1I	GGYRCC	1 cut(s)	521
AccII	CGCG	1 cut(s)	723
AciI	CCGC	2 cut(s)	366, 1657
AclWI	GGATC	6 cut(s)	496, 613, 656, 661, 1248, 1340
AcoI	YGGCCR	1 cut(s)	67
AcsI	RAATTY	3 cut(s)	556, 852, 1103
AcuI	CTGAAG	2 cut(s)	696, 1623
AcyI	GRCGYC	1 cut(s)	96
AfiI	CCNNNNNNNGG	1 cut(s)	386
AflII	CTTAAG	1 cut(s)	985
AgsI	TTSAA	7 cut(s)	53, 554, 561, 794, 828, 868, 940
AjiI	CACGTC	1 cut(s)	243
AjnI	CCWGG	1 cut(s)	352
AloI	GAACNNNNNNTCC	2 cut(s)	176, 208
Alw21I	GWGCWC	1 cut(s)	620
Alw26I	GTCTC	4 cut(s)	429, 466, 533, 754
AlwI	GGATC	6 cut(s)	496, 613, 656, 661, 1248, 1340
AlwNI	CAGNNNCTG	1 cut(s)	1343
AoxI	GGCC	3 cut(s)	61, 67, 350
ApeKI	GCWGC	4 cut(s)	207, 337, 1331, 1334
ApoI	RAATTY	3 cut(s)	556, 852, 1103
AspS9I	GGNCC	3 cut(s)	62, 350, 1159
AsuC2I	CCSGG	1 cut(s)	66
AsuHPI	GGTGA	1 cut(s)	917
AsuII	TTCGAA	1 cut(s)	1576
AsuNHI	GCTAGC	1 cut(s)	1430
AvaII	GGWCC	1 cut(s)	1159
BaeGI	GKGCMC	1 cut(s)	524
BanI	GGYRCC	1 cut(s)	521
BarI	GAAGNNNNNNTAC	4 cut(s)	877, 909, 1032, 1064
BbsI	GAAGAC	2 cut(s)	875, 892
Bbv12I	GWGCWC	1 cut(s)	620
BbvI	GCAGC	4 cut(s)	219, 324, 1318, 1321
BccI	CCATC	9 cut(s)	47, 113, 175, 475, 761, 896, 956, 1119, 1630
BceAI	ACGGC	1 cut(s)	462
BciT130I	CCWGG	1 cut(s)	354
BciVI	GTATCC	1 cut(s)	1400
BclI	TGATCA	4 cut(s)	571, 949, 1204, 1228
BcnI	CCSGG	1 cut(s)	66
BcoDI	GTCTC	4 cut(s)	429, 466, 533, 754
BfaI	CTAG	4 cut(s)	35, 416, 1431, 1547
BfmI	CTRYAG	3 cut(s)	338, 1323, 1335
BfrI	CTTAAG	1 cut(s)	985
BfuI	GTATCC	1 cut(s)	1400
BglII	AGATCT	2 cut(s)	342, 989
BisI	GCNGC	4 cut(s)	208, 338, 1332, 1335
BlpI	GCTNAGC	1 cut(s)	1140
BlsI	GCNGC	4 cut(s)	209, 339, 1333, 1336
Bme1390I	CCNGG	2 cut(s)	66, 354
Bme18I	GGWCC	1 cut(s)	1159
BmgBI	CACGTC	1 cut(s)	243
BmgT120I	GGNCC	3 cut(s)	62, 350, 1159
BmiI	GGNNCC	2 cut(s)	64, 523
BmrFI	CCNGG	2 cut(s)	66, 354
BmsI	GCATC	5 cut(s)	366, 391, 428, 1348, 1499
BmtI	GCTAGC	1 cut(s)	1434
BpiI	GAAGAC	2 cut(s)	875, 892
BpmI	CTGGAG	3 cut(s)	453, 630, 1420
Bpu1102I	GCTNAGC	1 cut(s)	1140
Bpu14I	TTCGAA	1 cut(s)	1576
BpuEI	CTTGAG	1 cut(s)	1105
BpuMI	CCSGG	1 cut(s)	66
Bsa29I	ATCGAT	1 cut(s)	80
BsaHI	GRCGYC	1 cut(s)	96
BsaI	GGTCTC	1 cut(s)	466
BsaJI	CCNNGG	4 cut(s)	64, 797, 1162, 1175
BsaXI	ACNNNNNCTCC	2 cut(s)	15, 45
Bsc4I	CCNNNNNNNGG	1 cut(s)	386
Bse118I	RCCGGY	1 cut(s)	59
Bse1I	ACTGG	5 cut(s)	607, 685, 1009, 1437, 1650
BseBI	CCWGG	1 cut(s)	354
BseCI	ATCGAT	1 cut(s)	80
BseDI	CCNNGG	4 cut(s)	64, 797, 1162, 1175
BseGI	GGATG	3 cut(s)	967, 1270, 1683
BseLI	CCNNNNNNNGG	1 cut(s)	386
BseMII	CTCAG	4 cut(s)	477, 993, 1497, 1611
BseNI	ACTGG	5 cut(s)	607, 685, 1009, 1437, 1650
BseSI	GKGCMC	1 cut(s)	524
BseXI	GCAGC	4 cut(s)	219, 324, 1318, 1321
BsgI	GTGCAG	1 cut(s)	627
Bsh1236I	CGCG	1 cut(s)	723
Bsh1285I	CGRYCG	2 cut(s)	80, 1181
BshFI	GGCC	3 cut(s)	63, 69, 352
BshNI	GGYRCC	1 cut(s)	521
BshVI	ATCGAT	1 cut(s)	80
BsiEI	CGRYCG	2 cut(s)	80, 1181
BsiHKAI	GWGCWC	1 cut(s)	620
BsiSI	CCGG	3 cut(s)	60, 66, 1471
BslFI	GGGAC	1 cut(s)	83
BslI	CCNNNNNNNGG	1 cut(s)	386
BsmAI	GTCTC	4 cut(s)	429, 466, 533, 754
BsmFI	GGGAC	1 cut(s)	83
BsmI	GAATGC	1 cut(s)	1629
BsnI	GGCC	3 cut(s)	63, 69, 352
Bso31I	GGTCTC	1 cut(s)	466
Bsp119I	TTCGAA	1 cut(s)	1576
Bsp1286I	GDGCHC	2 cut(s)	524, 620
Bsp1720I	GCTNAGC	1 cut(s)	1140
Bsp19I	CCATGG	1 cut(s)	797
BspACI	CCGC	2 cut(s)	366, 1657
BspANI	GGCC	3 cut(s)	63, 69, 352
BspCNI	CTCAG	4 cut(s)	476, 992, 1498, 1610
BspDI	ATCGAT	1 cut(s)	80
BspFNI	CGCG	1 cut(s)	723
BspHI	TCATGA	2 cut(s)	651, 1078
BspLI	GGNNCC	2 cut(s)	64, 523
BspMAI	CTGCAG	2 cut(s)	342, 1339
BspOI	GCTAGC	1 cut(s)	1434
BspPI	GGATC	6 cut(s)	496, 613, 656, 661, 1248, 1340
BspT104I	TTCGAA	1 cut(s)	1576
BspT107I	GGYRCC	1 cut(s)	521
BspTI	CTTAAG	1 cut(s)	985
BspTNI	GGTCTC	1 cut(s)	466
BsrFI	RCCGGY	1 cut(s)	59
BsrI	ACTGG	5 cut(s)	607, 685, 1009, 1437, 1650
BssAI	RCCGGY	1 cut(s)	59
BssECI	CCNNGG	4 cut(s)	64, 797, 1162, 1175
BssNI	GRCGYC	1 cut(s)	96
BssT1I	CCWWGG	1 cut(s)	797
Bst2UI	CCWGG	1 cut(s)	354
Bst4CI	ACNGT	4 cut(s)	165, 250, 1163, 1179
Bst6I	CTCTTC	1 cut(s)	803
BstACI	GRCGYC	1 cut(s)	96
BstAFI	CTTAAG	1 cut(s)	985
BstBI	TTCGAA	1 cut(s)	1576
BstC8I	GCNNGC	3 cut(s)	61, 1287, 1432
BstDEI	CTNAG	8 cut(s)	346, 463, 979, 1140, 1278, 1506, 1597, 1755
BstDSI	CCRYGG	3 cut(s)	797, 1162, 1175
BstEII	GGTNACC	2 cut(s)	905, 965
BstENI	CCTNNNNNAGG	1 cut(s)	384
BstF5I	GGATG	3 cut(s)	967, 1270, 1683
BstFNI	CGCG	1 cut(s)	723
BstMAI	GTCTC	4 cut(s)	429, 466, 533, 754
BstMCI	CGRYCG	2 cut(s)	80, 1181
BstMWI	GCNNNNNNNGC	4 cut(s)	363, 1295, 1340, 1496
BstNI	CCWGG	1 cut(s)	354
BstNSI	RCATGY	2 cut(s)	842, 1289
BstPI	GGTNACC	2 cut(s)	905, 965
BstSCI	CCNGG	2 cut(s)	64, 352
BstSFI	CTRYAG	3 cut(s)	338, 1323, 1335
BstSLI	GKGCMC	1 cut(s)	524
BstUI	CGCG	1 cut(s)	723
BstV1I	GCAGC	4 cut(s)	219, 324, 1318, 1321
BstV2I	GAAGAC	2 cut(s)	875, 892
BstX2I	RGATCY	3 cut(s)	342, 605, 989
BstYI	RGATCY	3 cut(s)	342, 605, 989
Bsu15I	ATCGAT	1 cut(s)	80
BsuI	GTATCC	1 cut(s)	1400
BsuRI	GGCC	3 cut(s)	63, 69, 352
BsuTUI	ATCGAT	1 cut(s)	80
BtgI	CCRYGG	3 cut(s)	797, 1162, 1175
BtrI	CACGTC	1 cut(s)	243
BtsCI	GGATG	3 cut(s)	967, 1270, 1683
BtsIMutI	CAGTG	3 cut(s)	114, 1444, 1527
Cac8I	GCNNGC	3 cut(s)	61, 1287, 1432
CaiI	CAGNNNCTG	1 cut(s)	1343
CciI	TCATGA	2 cut(s)	651, 1078
Cfr10I	RCCGGY	1 cut(s)	59
Cfr13I	GGNCC	3 cut(s)	62, 350, 1159
ClaI	ATCGAT	1 cut(s)	80
CviAII	CATG	8 cut(s)	237, 499, 652, 798, 839, 1079, 1286, 1587
DdeI	CTNAG	8 cut(s)	346, 463, 979, 1140, 1278, 1506, 1597, 1755
EaeI	YGGCCR	1 cut(s)	67
Eam1104I	CTCTTC	1 cut(s)	803
EarI	CTCTTC	1 cut(s)	803
Eco130I	CCWWGG	1 cut(s)	797
Eco31I	GGTCTC	1 cut(s)	466
Eco47I	GGWCC	1 cut(s)	1159
Eco57I	CTGAAG	2 cut(s)	696, 1623
Eco91I	GGTNACC	2 cut(s)	905, 965
EcoNI	CCTNNNNNAGG	1 cut(s)	384
EcoO109I	RGGNCCY	1 cut(s)	350
EcoO65I	GGTNACC	2 cut(s)	905, 965
EcoRII	CCWGG	1 cut(s)	352
EcoT14I	CCWWGG	1 cut(s)	797
ErhI	CCWWGG	1 cut(s)	797
FaeI	CATG	8 cut(s)	240, 502, 655, 801, 842, 1082, 1289, 1590
FaqI	GGGAC	1 cut(s)	83
FatI	CATG	8 cut(s)	236, 498, 651, 797, 838, 1078, 1285, 1586
FauNDI	CATATG	2 cut(s)	223, 1742
FbaI	TGATCA	4 cut(s)	571, 949, 1204, 1228
Fnu4HI	GCNGC	4 cut(s)	208, 338, 1332, 1335
FokI	GGATG	3 cut(s)	974, 1277, 1690
Fsp4HI	GCNGC	4 cut(s)	208, 338, 1332, 1335
FspBI	CTAG	4 cut(s)	35, 416, 1431, 1547
GluI	GCNGC	4 cut(s)	208, 338, 1332, 1335
GsuI	CTGGAG	3 cut(s)	453, 630, 1420
HaeIII	GGCC	3 cut(s)	63, 69, 352
HapII	CCGG	3 cut(s)	60, 66, 1471
Hin1I	GRCGYC	1 cut(s)	96
Hin1II	CATG	8 cut(s)	240, 502, 655, 801, 842, 1082, 1289, 1590
HincII	GTYRAC	2 cut(s)	246, 1366
HindII	GTYRAC	2 cut(s)	246, 1366
HinfI	GANTC	8 cut(s)	185, 331, 802, 956, 1005, 1082, 1116, 1171
HpaII	CCGG	3 cut(s)	60, 66, 1471
HphI	GGTGA	1 cut(s)	917
Hpy166II	GTNNAC	4 cut(s)	215, 246, 1366, 1570
Hpy8I	GTNNAC	4 cut(s)	215, 246, 1366, 1570
Hpy99I	CGWCG	1 cut(s)	365
HpyAV	CCTTC	3 cut(s)	1213, 1404, 1675
HpyCH4III	ACNGT	4 cut(s)	165, 250, 1163, 1179
HpyCH4IV	ACGT	3 cut(s)	96, 242, 1572
HpyCH4V	TGCA	9 cut(s)	17, 240, 340, 566, 644, 821, 1337, 1490, 1629
HpyF10VI	GCNNNNNNNGC	4 cut(s)	363, 1295, 1340, 1496
HpyF3I	CTNAG	8 cut(s)	346, 463, 979, 1140, 1278, 1506, 1597, 1755
HpySE526I	ACGT	3 cut(s)	96, 242, 1572
Hsp92I	GRCGYC	1 cut(s)	96
Hsp92II	CATG	8 cut(s)	240, 502, 655, 801, 842, 1082, 1289, 1590
KroI	GCCGGC	1 cut(s)	59
KroNI	GCCGGC	1 cut(s)	61
Ksp22I	TGATCA	4 cut(s)	571, 949, 1204, 1228
LmnI	GCTCC	3 cut(s)	1439, 1590, 1640
Lsp1109I	GCAGC	4 cut(s)	219, 324, 1318, 1321
LweI	GCATC	5 cut(s)	366, 391, 428, 1348, 1499
MaeI	CTAG	4 cut(s)	35, 416, 1431, 1547
MaeII	ACGT	3 cut(s)	96, 242, 1572
MaeIII	GTNAC	9 cut(s)	45, 112, 545, 687, 905, 965, 1447, 1522, 1532
MboII	GAAGA	6 cut(s)	256, 368, 820, 880, 897, 1393
MfeI	CAATTG	2 cut(s)	561, 863
MflI	RGATCY	3 cut(s)	342, 605, 989
MhlI	GDGCHC	2 cut(s)	524, 620
MlyI	GAGTC	2 cut(s)	179, 1076
MmeI	TCCRAC	1 cut(s)	448
MroNI	GCCGGC	1 cut(s)	59
MseI	TTAA	4 cut(s)	599, 986, 1131, 1619
MslI	CAYNNNNRTG	2 cut(s)	837, 1634
MspA1I	CMGCKG	2 cut(s)	210, 1601
MspCI	CTTAAG	1 cut(s)	985
MspI	CCGG	3 cut(s)	60, 66, 1471
MspR9I	CCNGG	2 cut(s)	66, 354
MunI	CAATTG	2 cut(s)	561, 863
Mva1269I	GAATGC	1 cut(s)	1629
MvaI	CCWGG	1 cut(s)	354
MvnI	CGCG	1 cut(s)	723
MwoI	GCNNNNNNNGC	4 cut(s)	363, 1295, 1340, 1496
NaeI	GCCGGC	1 cut(s)	61
NciI	CCSGG	1 cut(s)	66
NcoI	CCATGG	1 cut(s)	797
NdeI	CATATG	2 cut(s)	223, 1742
NgoMIV	GCCGGC	1 cut(s)	59
NheI	GCTAGC	1 cut(s)	1430
NlaIII	CATG	8 cut(s)	240, 502, 655, 801, 842, 1082, 1289, 1590
NlaIV	GGNNCC	2 cut(s)	64, 523
NmuCI	GTSAC	4 cut(s)	112, 545, 905, 1522
NspI	RCATGY	2 cut(s)	842, 1289
NspV	TTCGAA	1 cut(s)	1576
PaeI	GCATGC	1 cut(s)	1289
PagI	TCATGA	2 cut(s)	651, 1078
PctI	GAATGC	1 cut(s)	1629
PdiI	GCCGGC	1 cut(s)	61
PfeI	GAWTC	6 cut(s)	331, 802, 956, 1005, 1116, 1171
PkrI	GCNGC	4 cut(s)	209, 339, 1333, 1336
Ple19I	CGATCG	1 cut(s)	80
PleI	GAGTC	2 cut(s)	179, 1076
PpsI	GAGTC	2 cut(s)	179, 1076
Psp6I	CCWGG	1 cut(s)	352
PspEI	GGTNACC	2 cut(s)	905, 965
PspGI	CCWGG	1 cut(s)	352
PspN4I	GGNNCC	2 cut(s)	64, 523
PspPI	GGNCC	3 cut(s)	62, 350, 1159
PstI	CTGCAG	2 cut(s)	342, 1339
PstNI	CAGNNNCTG	1 cut(s)	1343
PsuI	RGATCY	3 cut(s)	342, 605, 989
PvuI	CGATCG	1 cut(s)	80
PvuII	CAGCTG	2 cut(s)	210, 1601
RseI	CAYNNNNRTG	2 cut(s)	837, 1634
SaqAI	TTAA	4 cut(s)	599, 986, 1131, 1619
SatI	GCNGC	4 cut(s)	208, 338, 1332, 1335
Sau96I	GGNCC	3 cut(s)	62, 350, 1159
SchI	GAGTC	2 cut(s)	179, 1076
ScrFI	CCNGG	2 cut(s)	66, 354
SduI	GDGCHC	2 cut(s)	524, 620
SfaNI	GCATC	5 cut(s)	366, 391, 428, 1348, 1499
SfcI	CTRYAG	3 cut(s)	338, 1323, 1335
SfuI	TTCGAA	1 cut(s)	1576
SinI	GGWCC	1 cut(s)	1159
SmiMI	CAYNNNNRTG	2 cut(s)	837, 1634
SmlI	CTYRAG	2 cut(s)	985, 1120
SmoI	CTYRAG	2 cut(s)	985, 1120
SphI	GCATGC	1 cut(s)	1289
SsiI	CCGC	2 cut(s)	366, 1657
SspMI	CTAG	4 cut(s)	35, 416, 1431, 1547
StyD4I	CCNGG	2 cut(s)	64, 352
StyI	CCWWGG	1 cut(s)	797
TaaI	ACNGT	4 cut(s)	165, 250, 1163, 1179
TaiI	ACGT	3 cut(s)	99, 245, 1575
TaqI	TCGA	4 cut(s)	76, 80, 1181, 1576
TfiI	GAWTC	6 cut(s)	331, 802, 956, 1005, 1116, 1171
Tru1I	TTAA	4 cut(s)	599, 986, 1131, 1619
Tru9I	TTAA	4 cut(s)	599, 986, 1131, 1619
TscAI	CASTG	3 cut(s)	121, 1444, 1527
TseFI	GTSAC	4 cut(s)	112, 545, 905, 1522
TseI	GCWGC	4 cut(s)	207, 337, 1331, 1334
Tsp45I	GTSAC	4 cut(s)	112, 545, 905, 1522
TspDTI	ATGAA	4 cut(s)	597, 1053, 1199, 1393
TspGWI	ACGGA	1 cut(s)	661
TspRI	CASTG	3 cut(s)	121, 1444, 1527
Vha464I	CTTAAG	1 cut(s)	985
VpaK11BI	GGWCC	1 cut(s)	1159
XagI	CCTNNNNNAGG	1 cut(s)	384
XapI	RAATTY	3 cut(s)	556, 852, 1103
XceI	RCATGY	2 cut(s)	842, 1289
XspI	CTAG	4 cut(s)	35, 416, 1431, 1547
ZraI	GACGTC	1 cut(s)	97

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.