Rw2G027040

Sodium/calcium exchanger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015661
Physical Location & Seq
Forward (+)
42098338 .. 42101820
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027040.1

Sequence Viewer

Length: 1737 bp
ATGGCAAAGAAAATTGCGTTATTCCTTTATTTCCTATCCATCATGCTAGTTCATCTAACCAGCTTTGGCCAAAGTCGTTCTATTGCTACAAGTGACTTCAATTTAGTGTCTGATGGAGTAAACCACACTACTGGGGATAAACCCAATTTTCTTTGGTTAAAGCATAGCTTGTTTGCATCCGATAGCTGTGATGAGACATATGGGTTCTTGCCATGCACCAACACAGTCCTTGGTAATATCTTCCTCATTATCGTCTATGGTTACTTGATGTTCCTTTCAGCCAAGTTATTGTCAGCTGGAAGTGAGATTCTGCTGCAAATTTTAGGACCTGGTATCATAGGGGGTCTCTTCTTGCCTCTGTTGAGTGCTGTACCTGATGCCACCATTATCCTTGCATCAGGACTTTCTGGGGACACGGCAACGGCACAAAGTCAGGTCTCCGTTGGAATGGGCTTGCTAGCTGGATCCAATGTAATGCTTCTCACAATCCTATGGGGAACCTGTCTTCTAGTTGGCAAATGTGACCTTGAAAATTCCGTTGCAGTTGATGAAAAAGATACAAAGCGTTTTAGCTTAAATGGATCTGGAGTGAGCACAGATATTTGGACAAGCTATGCTGCATGGATCATGGTTATCTCCATTATTCCTTTTGTAATAGTCCAACTACCTCAAGTTTTCACTGCGACTTTGGATAGTAGCTTGCCAATTCTAAGTTCACTCATTGTTGCAATCTGTTTTGTGATCGCTTATAGCATCTATCAGATTTTTCAGCCTTGGATTCAGAAGAGAAGACTTGCATATGCAAAGCATAAGCATGTGATGTCAGAGATTCTAAAACAGTTGAAAACAAACGCGCTTGGAAAATTGCTTACAGATGATGGTGATCCTAACAAAGTTGTTATAGAGAAGTTATTCAGGACACTTGATCAGAACTCGGATGGTTATTTGAACACCACAGACTTTAGATCTTTGATAATAGGAATCCAATTTGATGATGACGTAGATATAAATATCGATGAAGCCATTTCTCAAGTGATGAGAGACTTTGACACAACTCGTGATTCGAAGATTGATGTTGATGAATTCTTCAAAGGAATTTCAAGGTGGCTTAACAAGGCTAAGCGTGCTGCAATGATGGACCGTGGAAAGGCTCCGCTATCAATGAAACTGTTAGATGATTTTGATAAGCAAACAAAGGAAGAACAAGATCGGTATGGGGACCAGATTGATGAGATTGTGGAGGATGTTAAAAATGCAAACTGGCATGCCTCAAAAGCAGTACTGATGTTGCTTCTGGGAACCATTGTTGCTGCTGTGGTTGCTGATCCCCTTGTTGACGCTGTTGACAACTTCTCAACAGCTACAAGTATTCCTACATTCTTCGTCTCATTTGTTATTCTGCCTTTTGTTAGCTCCAGTGAGATTGTGTCCACTCTAATTTTTGTCAGCCGGAAGAAGCAGAGAACAGCATCATTGGCCTACTCTGCGATATATGGATCAGTAACAATGAGTAACATCCTCTCCCTGGCAGTGTTCTTGGCTCTTGTATACATTCGAAATCTGACATGGAGCTTCTCGTCCGAAGTTCTTGTCATTATAATTGTCTGCATTCTAATGGGAGTTGTTGCTGGTTTCCGCACCACCTTCCCTGTTTGGATGTCTTTTGGGGCTGTAGCACTCTATCCACTTTCTCTGTTGCTGGTTTACATTCTTGAATATGTTTTTGGGTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

578

Amino Acids

63.7

Weight (kDa)

5.2

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 80 - 257 2.3e-07 Sodium/calcium exchanger protein
EF-hand_7 PF13499 300 - 366 7.9e-11 EF-hand domain pair
Na_Ca_ex PF01699 426 - 569 3.1e-11 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015661)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26990 FvH4_6g26990 FvH4_6g26990 FvH4_6g26990
malus_domestica MD17G1233800.v1.1
prunus_persica Prupe.3G125200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0128851
rosa_laevigata RLG00000019033
rosa_multiflora Rmu_sc0001643.1_g000033
rosa_roxburghii Rroxscaffold_2G00115170
rosa_rugosa Rorug02G0281800 Rorug02G0281800
rosa_samantha Rh2AG334300 Rh2CG321200 Rh2DG359000
rosa_wichuraiana Rw2G027040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1598
AccI GTMKAC 1 cut(s) 1548
AccII CGCG 1 cut(s) 854
AciI CCGC 2 cut(s) 1154, 1636
AclWI GGATC 7 cut(s) 459, 472, 589, 632, 878, 1319, 1504
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 4 cut(s) 318, 532, 1082, 1095
AfaI GTAC 2 cut(s) 372, 1281
AfiI CCNNNNNNNGG 2 cut(s) 1147, 1525
AgsI TTSAA 7 cut(s) 100, 530, 844, 949, 1090, 1101, 1715
AjnI CCWGG 2 cut(s) 328, 1524
AjuI GAANNNNNNNTTGG 3 cut(s) 697, 729, 1707
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 5 cut(s) 188, 350, 442, 1035, 1390
AlwI GGATC 7 cut(s) 459, 472, 589, 632, 878, 1319, 1504
AoxI GGCC 2 cut(s) 67, 1476
ApeKI GCWGC 4 cut(s) 313, 617, 1127, 1310
ApoI RAATTY 4 cut(s) 318, 532, 1082, 1095
ArsI GACNNNNNNTTYG 2 cut(s) 1575, 1607
Asp700I GAANNNNTTC 1 cut(s) 911
AspLEI GCGC 1 cut(s) 856
AspS9I GGNCC 3 cut(s) 326, 1138, 1219
AsuHPI GGTGA 1 cut(s) 893
AsuII TTCGAA 2 cut(s) 1064, 1555
AsuNHI GCTAGC 1 cut(s) 457
AvaII GGWCC 3 cut(s) 326, 1138, 1219
BalI TGGCCA 1 cut(s) 69
BamHI GGATCC 1 cut(s) 464
BauI CACGAG 1 cut(s) 1056
BbsI GAAGAC 2 cut(s) 497, 796
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 4 cut(s) 300, 604, 1114, 1297
BccI CCATC 5 cut(s) 47, 107, 872, 932, 1129
BceAI ACGGC 2 cut(s) 432, 438
BciT130I CCWGG 2 cut(s) 330, 1526
BclI TGATCA 1 cut(s) 925
BcoDI GTCTC 5 cut(s) 188, 350, 442, 1035, 1390
BfaI CTAG 3 cut(s) 47, 458, 509
BfmI CTRYAG 1 cut(s) 1671
BglII AGATCT 1 cut(s) 965
BisI GCNGC 4 cut(s) 314, 618, 1128, 1311
BlpI GCTNAGC 1 cut(s) 1119
BlsI GCNGC 4 cut(s) 315, 619, 1129, 1312
BmcAI AGTACT 1 cut(s) 1281
Bme1390I CCNGG 2 cut(s) 330, 1526
Bme18I GGWCC 3 cut(s) 326, 1138, 1219
BmgT120I GGNCC 3 cut(s) 326, 1138, 1219
BmiI GGNNCC 5 cut(s) 466, 499, 1152, 1220, 1300
BmrFI CCNGG 2 cut(s) 330, 1526
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 5 cut(s) 185, 367, 404, 762, 1478
BmtI GCTAGC 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 497, 796
BpmI CTGGAG 2 cut(s) 606, 1399
Bpu1102I GCTNAGC 1 cut(s) 1119
Bpu14I TTCGAA 2 cut(s) 1064, 1555
BpuEI CTTGAG 2 cut(s) 654, 1014
Bsa29I ATCGAT 1 cut(s) 1014
BsaBI GATNNNNATC 1 cut(s) 882
BsaI GGTCTC 2 cut(s) 350, 442
BsaJI CCNNGG 4 cut(s) 229, 773, 1141, 1524
BsaXI ACNNNNNCTCC 2 cut(s) 1505, 1535
Bsc4I CCNNNNNNNGG 2 cut(s) 1147, 1525
Bse1I ACTGG 3 cut(s) 136, 1265, 1416
Bse3DI GCAATG 1 cut(s) 1137
Bse8I GATNNNNATC 1 cut(s) 882
BseBI CCWGG 2 cut(s) 330, 1526
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 4 cut(s) 229, 773, 1141, 1524
BseGI GGATG 5 cut(s) 176, 943, 1249, 1515, 1662
BseJI GATNNNNATC 1 cut(s) 882
BseLI CCNNNNNNNGG 2 cut(s) 1147, 1525
BseMI GCAATG 1 cut(s) 1137
BseNI ACTGG 3 cut(s) 136, 1265, 1416
BseXI GCAGC 4 cut(s) 300, 604, 1114, 1297
Bsh1236I CGCG 1 cut(s) 854
BshFI GGCC 2 cut(s) 69, 1478
BshVI ATCGAT 1 cut(s) 1014
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 1 cut(s) 1450
BslFI GGGAC 2 cut(s) 425, 1232
BslI CCNNNNNNNGG 2 cut(s) 1147, 1525
BsmAI GTCTC 5 cut(s) 188, 350, 442, 1035, 1390
BsmBI CGTCTC 1 cut(s) 1390
BsmFI GGGAC 2 cut(s) 425, 1232
BsmI GAATGC 1 cut(s) 1608
BsnI GGCC 2 cut(s) 69, 1478
Bso31I GGTCTC 2 cut(s) 350, 442
Bsp119I TTCGAA 2 cut(s) 1064, 1555
Bsp1286I GDGCHC 1 cut(s) 596
Bsp1720I GCTNAGC 1 cut(s) 1119
BspACI CCGC 2 cut(s) 1154, 1636
BspANI GGCC 2 cut(s) 69, 1478
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 1 cut(s) 854
BspLI GGNNCC 5 cut(s) 466, 499, 1152, 1220, 1300
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 7 cut(s) 459, 472, 589, 632, 878, 1319, 1504
BspT104I TTCGAA 2 cut(s) 1064, 1555
BspTNI GGTCTC 2 cut(s) 350, 442
BsrDI GCAATG 1 cut(s) 1137
BsrI ACTGG 3 cut(s) 136, 1265, 1416
BssECI CCNNGG 4 cut(s) 229, 773, 1141, 1524
BssNAI GTATAC 1 cut(s) 1549
BssSI CACGAG 1 cut(s) 1056
BssT1I CCWWGG 2 cut(s) 229, 773
Bst1107I GTATAC 1 cut(s) 1549
Bst2BI CACGAG 1 cut(s) 1056
Bst2UI CCWGG 2 cut(s) 330, 1526
Bst4CI ACNGT 4 cut(s) 226, 840, 1142, 1170
Bst6I CTCTTC 2 cut(s) 353, 779
BstBI TTCGAA 2 cut(s) 1064, 1555
BstC8I GCNNGC 5 cut(s) 455, 459, 701, 1125, 1266
BstDEI CTNAG 2 cut(s) 710, 1119
BstDSI CCRYGG 1 cut(s) 1141
BstF5I GGATG 5 cut(s) 176, 943, 1249, 1515, 1662
BstFNI CGCG 1 cut(s) 854
BstHHI GCGC 1 cut(s) 856
BstMAI GTCTC 5 cut(s) 188, 350, 442, 1035, 1390
BstMWI GCNNNNNNNGC 5 cut(s) 1124, 1274, 1319, 1475, 1484
BstNI CCWGG 2 cut(s) 330, 1526
BstNSI RCATGY 2 cut(s) 818, 1268
BstSCI CCNGG 2 cut(s) 328, 1524
BstSFI CTRYAG 1 cut(s) 1671
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 4 cut(s) 300, 604, 1114, 1297
BstV2I GAAGAC 2 cut(s) 497, 796
BstX2I RGATCY 3 cut(s) 464, 581, 965
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 3 cut(s) 464, 581, 965
BstZ17I GTATAC 1 cut(s) 1549
Bsu15I ATCGAT 1 cut(s) 1014
BsuRI GGCC 2 cut(s) 69, 1478
BsuTUI ATCGAT 1 cut(s) 1014
BtgI CCRYGG 1 cut(s) 1141
BtsCI GGATG 5 cut(s) 176, 943, 1249, 1515, 1662
BtsI GCAGTG 2 cut(s) 678, 1536
BtsIMutI CAGTG 3 cut(s) 678, 1423, 1536
Cac8I GCNNGC 5 cut(s) 455, 459, 701, 1125, 1266
CfoI GCGC 1 cut(s) 856
Cfr13I GGNCC 3 cut(s) 326, 1138, 1219
ClaI ATCGAT 1 cut(s) 1014
CseI GACGC 1 cut(s) 1346
CsiI ACCWGGT 1 cut(s) 328
Csp6I GTAC 2 cut(s) 371, 1280
CviAII CATG 7 cut(s) 43, 213, 621, 628, 815, 1265, 1566
CviQI GTAC 2 cut(s) 371, 1280
DdeI CTNAG 2 cut(s) 710, 1119
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 353, 779
EarI CTCTTC 2 cut(s) 353, 779
Eco130I CCWWGG 2 cut(s) 229, 773
Eco31I GGTCTC 2 cut(s) 350, 442
Eco47I GGWCC 3 cut(s) 326, 1138, 1219
EcoO109I RGGNCCY 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 1082
EcoRII CCWGG 2 cut(s) 328, 1524
EcoT14I CCWWGG 2 cut(s) 229, 773
ErhI CCWWGG 2 cut(s) 229, 773
Esp3I CGTCTC 1 cut(s) 1390
FaeI CATG 7 cut(s) 46, 216, 624, 631, 818, 1268, 1569
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 2 cut(s) 425, 1232
FatI CATG 7 cut(s) 42, 212, 620, 627, 814, 1264, 1565
FauNDI CATATG 2 cut(s) 199, 799
FbaI TGATCA 1 cut(s) 925
FblI GTMKAC 1 cut(s) 1548
Fnu4HI GCNGC 4 cut(s) 314, 618, 1128, 1311
FokI GGATG 5 cut(s) 163, 950, 1256, 1502, 1669
Fsp4HI GCNGC 4 cut(s) 314, 618, 1128, 1311
FspBI CTAG 3 cut(s) 47, 458, 509
GlaI GCGC 1 cut(s) 855
GluI GCNGC 4 cut(s) 314, 618, 1128, 1311
GsuI CTGGAG 2 cut(s) 606, 1399
HaeIII GGCC 2 cut(s) 69, 1478
HapII CCGG 1 cut(s) 1450
HgaI GACGC 1 cut(s) 1346
HhaI GCGC 1 cut(s) 856
Hin1II CATG 7 cut(s) 46, 216, 624, 631, 818, 1268, 1569
Hin6I GCGC 1 cut(s) 854
HinP1I GCGC 1 cut(s) 854
HincII GTYRAC 2 cut(s) 1336, 1345
HindII GTYRAC 2 cut(s) 1336, 1345
HinfI GANTC 5 cut(s) 307, 778, 829, 981, 1061
HpaII CCGG 1 cut(s) 1450
HphI GGTGA 1 cut(s) 893
Hpy166II GTNNAC 7 cut(s) 121, 716, 1336, 1345, 1431, 1549, 1705
Hpy188I TCNGA 9 cut(s) 112, 181, 762, 783, 826, 930, 937, 1563, 1582
Hpy188III TCNNGA 5 cut(s) 399, 585, 916, 1058, 1712
Hpy8I GTNNAC 7 cut(s) 121, 716, 1336, 1345, 1431, 1549, 1705
HpyAV CCTTC 1 cut(s) 1654
HpyCH4III ACNGT 4 cut(s) 226, 840, 1142, 1170
HpyCH4IV ACGT 1 cut(s) 999
HpyF10VI GCNNNNNNNGC 5 cut(s) 1124, 1274, 1319, 1475, 1484
HpyF3I CTNAG 2 cut(s) 710, 1119
HpySE526I ACGT 1 cut(s) 999
Hsp92II CATG 7 cut(s) 46, 216, 624, 631, 818, 1268, 1569
HspAI GCGC 1 cut(s) 854
Ksp22I TGATCA 1 cut(s) 925
LmnI GCTCC 3 cut(s) 1156, 1418, 1569
Lsp1109I GCAGC 4 cut(s) 300, 604, 1114, 1297
LweI GCATC 5 cut(s) 185, 367, 404, 762, 1478
MabI ACCWGGT 1 cut(s) 328
MaeI CTAG 3 cut(s) 47, 458, 509
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 5 cut(s) 92, 260, 521, 1501, 1511
MflI RGATCY 3 cut(s) 464, 581, 965
MhlI GDGCHC 1 cut(s) 596
MlsI TGGCCA 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 69
MmeI TCCRAC 2 cut(s) 424, 685
MnlI CCTC 6 cut(s) 254, 366, 678, 1234, 1279, 1529
Mox20I TGGCCA 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 911
MscI TGGCCA 1 cut(s) 69
MseI TTAA 4 cut(s) 158, 575, 1110, 1248
MslI CAYNNNNRTG 2 cut(s) 813, 1613
Msp20I TGGCCA 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 1450
MspR9I CCNGG 2 cut(s) 330, 1526
Mva1269I GAATGC 1 cut(s) 1608
MvaI CCWGG 2 cut(s) 330, 1526
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 5 cut(s) 1124, 1274, 1319, 1475, 1484
NdeI CATATG 2 cut(s) 199, 799
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 7 cut(s) 46, 216, 624, 631, 818, 1268, 1569
NlaIV GGNNCC 5 cut(s) 466, 499, 1152, 1220, 1300
NmuCI GTSAC 2 cut(s) 92, 521
NspI RCATGY 2 cut(s) 818, 1268
NspV TTCGAA 2 cut(s) 1064, 1555
PaeI GCATGC 1 cut(s) 1268
PctI GAATGC 1 cut(s) 1608
PdmI GAANNNNTTC 1 cut(s) 911
PfeI GAWTC 5 cut(s) 307, 778, 829, 981, 1061
PkrI GCNGC 4 cut(s) 315, 619, 1129, 1312
PpuMI RGGWCCY 1 cut(s) 326
PsiI TTATAA 1 cut(s) 1598
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 328, 1524
PspGI CCWGG 2 cut(s) 328, 1524
PspN4I GGNNCC 5 cut(s) 466, 499, 1152, 1220, 1300
PspPI GGNCC 3 cut(s) 326, 1138, 1219
PspPPI RGGWCCY 1 cut(s) 326
PsuI RGATCY 3 cut(s) 464, 581, 965
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 2 cut(s) 372, 1281
RsaNI GTAC 2 cut(s) 371, 1280
RseI CAYNNNNRTG 2 cut(s) 813, 1613
SaqAI TTAA 4 cut(s) 158, 575, 1110, 1248
SatI GCNGC 4 cut(s) 314, 618, 1128, 1311
Sau96I GGNCC 3 cut(s) 326, 1138, 1219
ScaI AGTACT 1 cut(s) 1281
ScrFI CCNGG 2 cut(s) 330, 1526
SduI GDGCHC 1 cut(s) 596
SexAI ACCWGGT 1 cut(s) 328
SfaNI GCATC 5 cut(s) 185, 367, 404, 762, 1478
SfcI CTRYAG 1 cut(s) 1671
SfuI TTCGAA 2 cut(s) 1064, 1555
SinI GGWCC 3 cut(s) 326, 1138, 1219
SmiMI CAYNNNNRTG 2 cut(s) 813, 1613
SmlI CTYRAG 2 cut(s) 669, 1029
SmoI CTYRAG 2 cut(s) 669, 1029
SphI GCATGC 1 cut(s) 1268
SsiI CCGC 2 cut(s) 1154, 1636
SspMI CTAG 3 cut(s) 47, 458, 509
StyD4I CCNGG 2 cut(s) 328, 1524
StyI CCWWGG 2 cut(s) 229, 773
TaaI ACNGT 4 cut(s) 226, 840, 1142, 1170
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 3 cut(s) 1014, 1064, 1555
TatI WGTACW 1 cut(s) 1279
TfiI GAWTC 5 cut(s) 307, 778, 829, 981, 1061
Tru1I TTAA 4 cut(s) 158, 575, 1110, 1248
Tru9I TTAA 4 cut(s) 158, 575, 1110, 1248
TscAI CASTG 3 cut(s) 685, 1423, 1536
TseFI GTSAC 2 cut(s) 92, 521
TseI GCWGC 4 cut(s) 313, 617, 1127, 1310
Tsp45I GTSAC 2 cut(s) 92, 521
TspDTI ATGAA 5 cut(s) 41, 564, 1032, 1095, 1178
TspGWI ACGGA 2 cut(s) 430, 526
TspRI CASTG 3 cut(s) 685, 1423, 1536
VpaK11BI GGWCC 3 cut(s) 326, 1138, 1219
XapI RAATTY 4 cut(s) 318, 532, 1082, 1095
XceI RCATGY 2 cut(s) 818, 1268
XmiI GTMKAC 1 cut(s) 1548
XmnI GAANNNNTTC 1 cut(s) 911
XspI CTAG 3 cut(s) 47, 458, 509
ZrmI AGTACT 1 cut(s) 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.