Prupe.3G125200_v2.0.a1

Sodium/calcium exchanger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0015661
Physical Location & Seq
Reverse (-)
11504627 .. 11509235
4609 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G125200.1

Sequence Viewer

Length: 1728 bp
ATGGCAAAGAAACTCACACTCCTTTTGCTAGCCATCTTGTTACTTCAACAAGCCAGCTCCGGCGAAAGTCGGTCCATAACAAACGACTTGCCGTTGAGCATGGTCTCCGATGGAGTACTCGAAAAAACTCCGAAACCAAATTCCATTTGGCTGCCGGGCCGCTTGTTCTCTTCAGATAGTTGTGACCAGACATACGGGTTCTTGCCATGCACAACCACAGTTTTGGGAAATACTTTCCTGATCATCGTTTATGGCTACTTGATGTTTCTATCAGCCAAGCTGTTGTCTAATGGAAGTGAGATTCTGCTGCAGATTTTAGGGCCTGGTATCGTTGGTGGTTTCTTCCTGCCTCTTTTGAGCTCTATACCTGATGCCACCATTATTCTTGCATCTGGACTTTCTGGAGACACAGAAACGGCTCAAAGTCAGGTCTCTGTGGGAATGGGCTTGCTAGCTGGATCAAATGTTATGCTTCTAACAATTCTATGGGGCACCTGTCTCCTAGTTGGCAAATGTGACCTTGAAAATTCAGTTGCAGTTGATCAAAAAGATGAAAAACGATTTAGCTTAACTGGATCTGGAGTGAGCACAGATATTTGGACAAGCTATGCTGCAAGGATCATGGTTTTCTCTATTATTCCTTTTGTAATTGTCCAATTACCCCAAGTTTTTCACAGAACTTCTGACAATCGCTTAGCAATTCTGATTTCGCTCATCGTCGCCATCTCCTTTGTGATTGCTTATAGCCTCTATCAGGTCTTTCAGCCATGGATTCAGAAGAGAAAACTTGCATTATCAAAGCATAAGCATGTGATGTCAGAAATTCTAATACAGTTGAAAACAAATGCTCTGGGAAGACTTCTTACAAATGATGGTGATCCTAACAAAGTTGTTATAGAAAAGTTGTTCAAGACACTTGATCAGGATTCGGATGGTTATCTAAGTACCGCAGACTTAAGATCTCTGATTATAGGAATCCAGTTTGACGATATGGACATAAACATCGATGAAGCAATTTCACAAGTGATGAGAGATTTTGATACATCTCATGACTCAAAGATTGATGTGGATGAATTTTTCAAGGGTATCTCGAGATGGATCAACAAGGCTAAGCGTGTCGCAATAATGGAGCGTGGAAAGCTTCCACCATCGATGAAACTGTTAGAGGATTTTGATAAGAAAACAAAGAAAGAGCAGGATCAGTTTATGGATCAAATGGATGAGGTTGTGGAGGATGTTAAAAATGTCAAGTGGCATGCTTCCAAAGCAGTAGGGTTGCTACTTCTGGGAACTATAGTTGCTGCAGTGTTTGCTGATCCACTTGTTGATGCTGTTGACAACTTCTCAACTGCTACGAGTATCCCTTCGTTCTTCGTCTCATTCGTCATTCTACCTTTTGCTAGCTCCAGTGAGATAGTCTCCACTCTAATTTTCGTCAGCCGGAAAAAGCAGAGAACTGCCTCATTGGCATACTCTGAGATATATGGCTCAGTAACAATGAGCAACATCCTTTCCCTGGCAGTTTTCTTGGGTCTGGTTTACATTCGCAACCTGACGTGGAGCTTTTCTGCTGAAGTTCTCGTTATTGTAGTTGTCTGCATTCTAATGGGTGCCATTGCCAGTTTCCGCACCACCTTCCCCCTTTGGATGTCTCTGGTGGCTATGCTGCTTTATCCACTTTCTCTGTTGCTGGTTTATATTCTTGACTATGTATTTGGGTGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

576

Amino Acids

63.45

Weight (kDa)

5.56

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015661)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26990 FvH4_6g26990 FvH4_6g26990 FvH4_6g26990
malus_domestica MD17G1233800.v1.1
prunus_persica Prupe.3G125200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0128851
rosa_laevigata RLG00000019033
rosa_multiflora Rmu_sc0001643.1_g000033
rosa_roxburghii Rroxscaffold_2G00115170
rosa_rugosa Rorug02G0281800 Rorug02G0281800
rosa_samantha Rh2AG334300 Rh2CG321200 Rh2DG359000
rosa_wichuraiana Rw2G027040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 491, 1610
AciI CCGC 3 cut(s) 160, 948, 1627
AclWI GGATC 8 cut(s) 466, 583, 626, 872, 1106, 1206, 1218, 1310
AcsI RAATTY 4 cut(s) 139, 526, 822, 1073
AcuI CTGAAG 2 cut(s) 156, 1593
AfaI GTAC 2 cut(s) 117, 946
AfiI CCNNNNNNNGG 2 cut(s) 754, 1516
AflII CTTAAG 1 cut(s) 955
AgsI TTSAA 5 cut(s) 47, 524, 838, 910, 1081
AjiI CACGTC 1 cut(s) 1557
AjnI CCWGG 2 cut(s) 322, 1515
AluBI AGCT 9 cut(s) 57, 280, 360, 455, 567, 606, 1141, 1404, 1563
AluI AGCT 9 cut(s) 57, 280, 360, 455, 567, 606, 1141, 1404, 1563
Alw21I GWGCWC 2 cut(s) 362, 590
Alw26I GTCTC 7 cut(s) 109, 399, 436, 503, 1381, 1423, 1656
AlwI GGATC 8 cut(s) 466, 583, 626, 872, 1106, 1206, 1218, 1310
Ama87I CYCGRG 1 cut(s) 1090
AoxI GGCC 2 cut(s) 157, 320
ApeKI GCWGC 5 cut(s) 151, 307, 611, 1301, 1666
ApoI RAATTY 4 cut(s) 139, 526, 822, 1073
ArsI GACNNNNNNTTYG 1 cut(s) 1697
AspS9I GGNCC 3 cut(s) 72, 157, 320
AsuC2I CCSGG 1 cut(s) 156
AsuHPI GGTGA 1 cut(s) 887
AsuNHI GCTAGC 3 cut(s) 28, 451, 1400
AvaI CYCGRG 1 cut(s) 1090
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 2 cut(s) 491, 1610
BanII GRGCYC 1 cut(s) 362
BarI GAAGNNNNNNTAC 2 cut(s) 847, 879
BbsI GAAGAC 1 cut(s) 862
Bbv12I GWGCWC 2 cut(s) 362, 590
BbvI GCAGC 5 cut(s) 138, 294, 598, 1288, 1653
BccI CCATC 7 cut(s) 41, 104, 731, 866, 926, 1089, 1156
BceAI ACGGC 2 cut(s) 76, 432
BciT130I CCWGG 2 cut(s) 324, 1517
BciVI GTATCC 1 cut(s) 1370
BclI TGATCA 3 cut(s) 240, 541, 919
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 7 cut(s) 109, 399, 436, 503, 1381, 1423, 1656
BfaI CTAG 4 cut(s) 29, 452, 503, 1401
BfmI CTRYAG 3 cut(s) 308, 1293, 1302
BfrI CTTAAG 1 cut(s) 955
BfuI GTATCC 1 cut(s) 1370
BglI GCCNNNNNGGC 1 cut(s) 1466
BglII AGATCT 1 cut(s) 959
BisI GCNGC 6 cut(s) 152, 160, 308, 612, 1302, 1667
BlpI GCTNAGC 2 cut(s) 694, 1110
BlsI GCNGC 6 cut(s) 153, 161, 309, 613, 1303, 1668
BmcAI AGTACT 1 cut(s) 117
Bme1390I CCNGG 3 cut(s) 156, 324, 1517
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 1090
BmgBI CACGTC 1 cut(s) 1557
BmgT120I GGNCC 3 cut(s) 72, 157, 320
BmiI GGNNCC 2 cut(s) 493, 1612
BmrFI CCNGG 3 cut(s) 156, 324, 1517
BmsI GCATC 3 cut(s) 361, 398, 1318
BmtI GCTAGC 3 cut(s) 32, 455, 1404
BpiI GAAGAC 1 cut(s) 862
BplI GAGNNNNNCTC 2 cut(s) 1403, 1435
BpmI CTGGAG 3 cut(s) 423, 600, 1390
Bpu1102I GCTNAGC 2 cut(s) 694, 1110
BpuMI CCSGG 1 cut(s) 156
Bsa29I ATCGAT 2 cut(s) 1005, 1151
BsaBI GATNNNNATC 2 cut(s) 876, 936
BsaI GGTCTC 2 cut(s) 109, 436
BsaJI CCNNGG 2 cut(s) 767, 1515
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 754, 1516
Bse1I ACTGG 4 cut(s) 577, 979, 1407, 1620
Bse3DI GCAATG 1 cut(s) 1614
Bse8I GATNNNNATC 2 cut(s) 876, 936
BseBI CCWGG 2 cut(s) 324, 1517
BseCI ATCGAT 2 cut(s) 1005, 1151
BseDI CCNNGG 2 cut(s) 767, 1515
BseGI GGATG 6 cut(s) 937, 1075, 1225, 1240, 1506, 1653
BseJI GATNNNNATC 2 cut(s) 876, 936
BseLI CCNNNNNNNGG 2 cut(s) 754, 1516
BseMI GCAATG 1 cut(s) 1614
BseMII CTCAG 2 cut(s) 1467, 1503
BseNI ACTGG 4 cut(s) 577, 979, 1407, 1620
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 5 cut(s) 138, 294, 598, 1288, 1653
BshFI GGCC 2 cut(s) 159, 322
BshNI GGYRCC 2 cut(s) 491, 1610
BshVI ATCGAT 2 cut(s) 1005, 1151
BsiHKAI GWGCWC 2 cut(s) 362, 590
BsiHKCI CYCGRG 1 cut(s) 1090
BsiSI CCGG 3 cut(s) 60, 155, 1441
BslI CCNNNNNNNGG 2 cut(s) 754, 1516
BsmAI GTCTC 7 cut(s) 109, 399, 436, 503, 1381, 1423, 1656
BsmBI CGTCTC 1 cut(s) 1381
BsmI GAATGC 1 cut(s) 1599
BsnI GGCC 2 cut(s) 159, 322
Bso31I GGTCTC 2 cut(s) 109, 436
BsoBI CYCGRG 1 cut(s) 1090
Bsp1286I GDGCHC 3 cut(s) 362, 494, 590
Bsp1720I GCTNAGC 2 cut(s) 694, 1110
Bsp19I CCATGG 1 cut(s) 767
BspACI CCGC 3 cut(s) 160, 948, 1627
BspANI GGCC 2 cut(s) 159, 322
BspCNI CTCAG 2 cut(s) 1468, 1502
BspDI ATCGAT 2 cut(s) 1005, 1151
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 493, 1612
BspMAI CTGCAG 2 cut(s) 312, 1306
BspOI GCTAGC 3 cut(s) 32, 455, 1404
BspPI GGATC 8 cut(s) 466, 583, 626, 872, 1106, 1206, 1218, 1310
BspT107I GGYRCC 2 cut(s) 491, 1610
BspTI CTTAAG 1 cut(s) 955
BspTNI GGTCTC 2 cut(s) 109, 436
BsrDI GCAATG 1 cut(s) 1614
BsrI ACTGG 4 cut(s) 577, 979, 1407, 1620
BssECI CCNNGG 2 cut(s) 767, 1515
BssT1I CCWWGG 1 cut(s) 767
Bst2UI CCWGG 2 cut(s) 324, 1517
Bst4CI ACNGT 3 cut(s) 220, 834, 1161
Bst6I CTCTTC 2 cut(s) 175, 773
BstAFI CTTAAG 1 cut(s) 955
BstAPI GCANNNNNTGC 1 cut(s) 1310
BstC8I GCNNGC 6 cut(s) 30, 55, 449, 453, 1257, 1402
BstDEI CTNAG 6 cut(s) 694, 941, 1110, 1476, 1489, 1725
BstDSI CCRYGG 1 cut(s) 767
BstENI CCTNNNNNAGG 1 cut(s) 752
BstF5I GGATG 6 cut(s) 937, 1075, 1225, 1240, 1506, 1653
BstMAI GTCTC 7 cut(s) 109, 399, 436, 503, 1381, 1423, 1656
BstMWI GCNNNNNNNGC 4 cut(s) 1138, 1265, 1310, 1466
BstNI CCWGG 2 cut(s) 324, 1517
BstNSI RCATGY 2 cut(s) 812, 1259
BstSCI CCNGG 3 cut(s) 154, 322, 1515
BstSFI CTRYAG 3 cut(s) 308, 1293, 1302
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 5 cut(s) 138, 294, 598, 1288, 1653
BstV2I GAAGAC 1 cut(s) 862
BstX2I RGATCY 2 cut(s) 575, 959
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 2 cut(s) 575, 959
Bsu15I ATCGAT 2 cut(s) 1005, 1151
BsuI GTATCC 1 cut(s) 1370
BsuRI GGCC 2 cut(s) 159, 322
BsuTUI ATCGAT 2 cut(s) 1005, 1151
BtgI CCRYGG 1 cut(s) 767
BtrI CACGTC 1 cut(s) 1557
BtsCI GGATG 6 cut(s) 937, 1075, 1225, 1240, 1506, 1653
BtsI GCAGTG 1 cut(s) 1311
BtsIMutI CAGTG 2 cut(s) 1311, 1414
Cac8I GCNNGC 6 cut(s) 30, 55, 449, 453, 1257, 1402
CciI TCATGA 1 cut(s) 1048
Cfr13I GGNCC 3 cut(s) 72, 157, 320
ClaI ATCGAT 2 cut(s) 1005, 1151
Csp6I GTAC 2 cut(s) 116, 945
CviAII CATG 7 cut(s) 100, 207, 622, 768, 809, 1049, 1256
CviQI GTAC 2 cut(s) 116, 945
DdeI CTNAG 6 cut(s) 694, 941, 1110, 1476, 1489, 1725
Eam1104I CTCTTC 2 cut(s) 175, 773
EarI CTCTTC 2 cut(s) 175, 773
Ecl136II GAGCTC 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 767
Eco24I GRGCYC 1 cut(s) 362
Eco31I GGTCTC 2 cut(s) 109, 436
Eco47I GGWCC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 360
Eco57I CTGAAG 2 cut(s) 156, 1593
Eco88I CYCGRG 1 cut(s) 1090
EcoICRI GAGCTC 1 cut(s) 360
EcoNI CCTNNNNNAGG 1 cut(s) 752
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 2 cut(s) 322, 1515
EcoT14I CCWWGG 1 cut(s) 767
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 767
Esp3I CGTCTC 1 cut(s) 1381
FaeI CATG 7 cut(s) 103, 210, 625, 771, 812, 1052, 1259
FatI CATG 7 cut(s) 99, 206, 621, 767, 808, 1048, 1255
FbaI TGATCA 3 cut(s) 240, 541, 919
Fnu4HI GCNGC 6 cut(s) 152, 160, 308, 612, 1302, 1667
FokI GGATG 6 cut(s) 944, 1082, 1232, 1247, 1493, 1660
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 6 cut(s) 152, 160, 308, 612, 1302, 1667
FspBI CTAG 4 cut(s) 29, 452, 503, 1401
GluI GCNGC 6 cut(s) 152, 160, 308, 612, 1302, 1667
GsuI CTGGAG 3 cut(s) 423, 600, 1390
HaeIII GGCC 2 cut(s) 159, 322
HapII CCGG 3 cut(s) 60, 155, 1441
Hin1II CATG 7 cut(s) 103, 210, 625, 771, 812, 1052, 1259
HincII GTYRAC 1 cut(s) 1336
HindII GTYRAC 1 cut(s) 1336
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 5 cut(s) 301, 772, 926, 975, 1052
HpaII CCGG 3 cut(s) 60, 155, 1441
HphI GGTGA 1 cut(s) 887
Hpy166II GTNNAC 2 cut(s) 1336, 1540
Hpy8I GTNNAC 2 cut(s) 1336, 1540
Hpy99I CGWCG 1 cut(s) 722
HpyAV CCTTC 2 cut(s) 1374, 1645
HpyCH4III ACNGT 3 cut(s) 220, 834, 1161
HpyCH4IV ACGT 1 cut(s) 1556
HpyCH4V TGCA 8 cut(s) 210, 310, 389, 536, 614, 791, 1304, 1599
HpyF10VI GCNNNNNNNGC 4 cut(s) 1138, 1265, 1310, 1466
HpyF3I CTNAG 6 cut(s) 694, 941, 1110, 1476, 1489, 1725
HpySE526I ACGT 1 cut(s) 1556
Hsp92II CATG 7 cut(s) 103, 210, 625, 771, 812, 1052, 1259
Ksp22I TGATCA 3 cut(s) 240, 541, 919
LmnI GCTCC 4 cut(s) 62, 1129, 1409, 1560
Lsp1109I GCAGC 5 cut(s) 138, 294, 598, 1288, 1653
LweI GCATC 3 cut(s) 361, 398, 1318
MaeI CTAG 4 cut(s) 29, 452, 503, 1401
MaeII ACGT 1 cut(s) 1556
MaeIII GTNAC 4 cut(s) 39, 182, 515, 1492
MboII GAAGA 5 cut(s) 162, 334, 790, 867, 1363
MflI RGATCY 2 cut(s) 575, 959
MhlI GDGCHC 3 cut(s) 362, 494, 590
MlyI GAGTC 1 cut(s) 1046
MnlI CCTC 6 cut(s) 360, 758, 1159, 1216, 1225, 1471
MseI TTAA 3 cut(s) 569, 956, 1239
MslI CAYNNNNRTG 2 cut(s) 807, 1604
MspCI CTTAAG 1 cut(s) 955
MspI CCGG 3 cut(s) 60, 155, 1441
MspR9I CCNGG 3 cut(s) 156, 324, 1517
Mva1269I GAATGC 1 cut(s) 1599
MvaI CCWGG 2 cut(s) 324, 1517
MwoI GCNNNNNNNGC 4 cut(s) 1138, 1265, 1310, 1466
NciI CCSGG 1 cut(s) 156
NcoI CCATGG 1 cut(s) 767
NheI GCTAGC 3 cut(s) 28, 451, 1400
NlaIII CATG 7 cut(s) 103, 210, 625, 771, 812, 1052, 1259
NlaIV GGNNCC 2 cut(s) 493, 1612
NmuCI GTSAC 2 cut(s) 182, 515
NspI RCATGY 2 cut(s) 812, 1259
PaeI GCATGC 1 cut(s) 1259
PaeR7I CTCGAG 1 cut(s) 1090
PagI TCATGA 1 cut(s) 1048
PcsI WCGNNNNNNNCGW 1 cut(s) 1380
PctI GAATGC 1 cut(s) 1599
PfeI GAWTC 4 cut(s) 301, 772, 926, 975
PkrI GCNGC 6 cut(s) 153, 161, 309, 613, 1303, 1668
PleI GAGTC 1 cut(s) 1046
PpsI GAGTC 1 cut(s) 1046
Psp124BI GAGCTC 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 322, 1515
PspGI CCWGG 2 cut(s) 322, 1515
PspN4I GGNNCC 2 cut(s) 493, 1612
PspPI GGNCC 3 cut(s) 72, 157, 320
PstI CTGCAG 2 cut(s) 312, 1306
PsuI RGATCY 2 cut(s) 575, 959
RsaI GTAC 2 cut(s) 117, 946
RsaNI GTAC 2 cut(s) 116, 945
RseI CAYNNNNRTG 2 cut(s) 807, 1604
SacI GAGCTC 1 cut(s) 362
SaqAI TTAA 3 cut(s) 569, 956, 1239
SatI GCNGC 6 cut(s) 152, 160, 308, 612, 1302, 1667
Sau96I GGNCC 3 cut(s) 72, 157, 320
ScaI AGTACT 1 cut(s) 117
SchI GAGTC 1 cut(s) 1046
ScrFI CCNGG 3 cut(s) 156, 324, 1517
SduI GDGCHC 3 cut(s) 362, 494, 590
SfaNI GCATC 3 cut(s) 361, 398, 1318
SfcI CTRYAG 3 cut(s) 308, 1293, 1302
Sfr274I CTCGAG 1 cut(s) 1090
SinI GGWCC 1 cut(s) 72
SlaI CTCGAG 1 cut(s) 1090
SmiMI CAYNNNNRTG 2 cut(s) 807, 1604
SmlI CTYRAG 2 cut(s) 955, 1090
SmoI CTYRAG 2 cut(s) 955, 1090
SphI GCATGC 1 cut(s) 1259
SsiI CCGC 3 cut(s) 160, 948, 1627
SspMI CTAG 4 cut(s) 29, 452, 503, 1401
SstI GAGCTC 1 cut(s) 362
StyD4I CCNGG 3 cut(s) 154, 322, 1515
StyI CCWWGG 1 cut(s) 767
TaaI ACNGT 3 cut(s) 220, 834, 1161
TaiI ACGT 1 cut(s) 1559
TaqI TCGA 4 cut(s) 120, 1005, 1091, 1151
TaqII GACCGA 1 cut(s) 60
TatI WGTACW 1 cut(s) 115
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 4 cut(s) 301, 772, 926, 975
Tru1I TTAA 3 cut(s) 569, 956, 1239
Tru9I TTAA 3 cut(s) 569, 956, 1239
TscAI CASTG 2 cut(s) 1311, 1414
TseFI GTSAC 2 cut(s) 182, 515
TseI GCWGC 5 cut(s) 151, 307, 611, 1301, 1666
Tsp45I GTSAC 2 cut(s) 182, 515
TspDTI ATGAA 4 cut(s) 567, 1023, 1086, 1169
TspRI CASTG 2 cut(s) 1311, 1414
Vha464I CTTAAG 1 cut(s) 955
VpaK11BI GGWCC 1 cut(s) 72
XagI CCTNNNNNAGG 1 cut(s) 752
XapI RAATTY 4 cut(s) 139, 526, 822, 1073
XceI RCATGY 2 cut(s) 812, 1259
XcmI CCANNNNNNNNNTGG 1 cut(s) 144
XhoI CTCGAG 1 cut(s) 1090
XspI CTAG 4 cut(s) 29, 452, 503, 1401
ZrmI AGTACT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.