Rh2CG321200

Sodium/calcium exchanger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015661
Physical Location & Seq
Forward (+)
41285425 .. 41288907
3483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG321200.1

Sequence Viewer

Length: 1740 bp
ATGGCAAAGAAAATTGCATTATTCCTTTATTTCCTATCCATCATGCTAGTTAATCTAACCAGCTTTGGCCAAAGTCGTTCTATTGCTACAAGTGACTTCAATTTAGTGTCTGATGGAGTAAACCACACTACTGGGGATAAACCGAATTTTCTTTGGTTAAAGCATAGCTTGTTTGCATCTGATAGCTGTGATGAGACATATGGATTCTTGCCATGCACCAACACAGTCCTTGGTAATATCTTCCTCATTATCGTCTATGGTTACTTGATGTTCCTTTCAGCCAAGTTATTGTCAGCTGGAAGTGAGATTCTGCTGCAAATTTTAGGACCTGGTATCATAGGGGGTCTCTTCTTGCCTCTGTTGAGTGCTGTACCTGATGCCACCATTATCCTTGCATCAGGACTTTCTGGGGACACGGCAACGGCACAAAGTCAGGTCTCCGTTGGAATGGGCTTGCTAGCTGGATCCAATGTAATGCTTCTCACAATCCTATGGGGGACCTGTCTTCTAGTTGGCAAATGTGACCTTGAAAATTCAGTTGCAGTTGATGAAAAAGATACAAAGCGTTTTAGCTTAACTGGATCTGGAGTGAGCACAGATATTTGGACAAGCTATGCTGCATGGATCATGGTTATCTCCATTATTCCTTTTGTAATAGTCCAACTACCTCAAGTTTTCACTGCAACTTTGGATAGTAGCTTGCCAATTCTAAGTTCACTCATTGTTGCAATCTGTTTTGTGATTGCTTATAGCATCTATCAGATTTTTCAGCCTTGGATTCAGAAGAGAAGACTTGCATATGCAAAGCATAAGCATGTAATGTCAGAGATTCTAAAACAGTTGAAAACAAACGCGCTTGGAAAATTGCTTACAGATGATGGTGATCCTAACAAAGTTGTTATAGAGAAGTTATTCAGGACACTTGATCAGAACTCGGATGGTTATTTGAACACCACAGAGTTAAGATCTTTGATAATAGGAATCCAATTTGATGATGACGTAGATATAAATATCGATGAAGTCGTTTCTCAAGTAATGAGAGACTTTGACACAACTCGTGATTCGAAGATTGATGTTGATGAATTTTTCAAAGGAATTTCAAGGTGGCTTAACAAGGCTAAGCGTGCTGCTGCAATGATGGTCCGTGGAAAGGCTCAGCTATCAATGAAACTGTTAGATGATTTTGATAAGCAAACAAAGGAAGAACAAGATCGGTATGGGGATCAGATTGATGAGATTGTGGAGGATGTTAAAAATGCAAATTGGCATGCCTCAAAAGCAGTACTGATGTTGCTTCTGGGAACCATTGTTGCTGCTGTGGTTGCTGATCCCCTTGTTGATGCTGTTGACAACTTCTCAACAGCTACAAGTATTCCTACATTCTTCGTCTCATTTGTTATTCTGCCTTTTGTTAGCTCCAGTGAGATTGTGTCCACTCTAATTTTTGTCAGCCGGAAGAAGCAGAGAACAGCATCATTGGCCTACTCTGCGATATATGGATCAGTAACAATGAGTAACATCCTCTCCCTGGCAGTGTTCTTGGCTCTTGTATACATTCGAAATCTGACATGGAGCTTCTCGTCCGAAGTTCTTGTCATTATAATTGTCTGCATTCTAATGGGAGTTGTTGCTGGTTTCCGCACCACCTTCCCTGTTTGGATGTCTTTTGGGGCTGTAGCGCTCTATCCACTTTCTCTGTTGCTGGTTTACATTCTTGAATATGTTTTTGGGTGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

579

Amino Acids

63.75

Weight (kDa)

5.21

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 80 - 257 3.3e-07 Sodium/calcium exchanger protein
EF-hand_7 PF13499 300 - 366 3.7e-11 EF-hand domain pair
Na_Ca_ex PF01699 427 - 570 3.1e-11 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015661)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26990 FvH4_6g26990 FvH4_6g26990 FvH4_6g26990
malus_domestica MD17G1233800.v1.1
prunus_persica Prupe.3G125200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0128851
rosa_laevigata RLG00000019033
rosa_multiflora Rmu_sc0001643.1_g000033
rosa_roxburghii Rroxscaffold_2G00115170
rosa_rugosa Rorug02G0281800 Rorug02G0281800
rosa_samantha Rh2AG334300 Rh2CG321200 Rh2DG359000
rosa_wichuraiana Rw2G027040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1601
AccI GTMKAC 1 cut(s) 1551
AccII CGCG 1 cut(s) 854
AciI CCGC 1 cut(s) 1639
AclWI GGATC 8 cut(s) 459, 472, 589, 632, 878, 1230, 1322, 1507
AcoI YGGCCR 1 cut(s) 67
AcsI RAATTY 5 cut(s) 145, 318, 532, 1082, 1095
AfaI GTAC 2 cut(s) 372, 1284
AfeI AGCGCT 1 cut(s) 1680
AfiI CCNNNNNNNGG 2 cut(s) 1150, 1528
AgsI TTSAA 7 cut(s) 100, 530, 844, 949, 1090, 1101, 1718
AjnI CCWGG 2 cut(s) 328, 1527
AjuI GAANNNNNNNTTGG 3 cut(s) 697, 729, 1710
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 5 cut(s) 188, 350, 442, 1035, 1393
AlwI GGATC 8 cut(s) 459, 472, 589, 632, 878, 1230, 1322, 1507
Aor51HI AGCGCT 1 cut(s) 1680
AoxI GGCC 2 cut(s) 67, 1479
ApeKI GCWGC 5 cut(s) 313, 617, 1127, 1130, 1313
ApoI RAATTY 5 cut(s) 145, 318, 532, 1082, 1095
ArsI GACNNNNNNTTYG 2 cut(s) 1578, 1610
Asp700I GAANNNNTTC 1 cut(s) 911
AspLEI GCGC 2 cut(s) 856, 1681
AspS9I GGNCC 3 cut(s) 326, 498, 1141
AsuHPI GGTGA 1 cut(s) 893
AsuII TTCGAA 2 cut(s) 1064, 1558
AsuNHI GCTAGC 1 cut(s) 457
AvaII GGWCC 3 cut(s) 326, 498, 1141
BalI TGGCCA 1 cut(s) 69
BamHI GGATCC 1 cut(s) 464
BauI CACGAG 1 cut(s) 1056
BbsI GAAGAC 2 cut(s) 497, 796
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 5 cut(s) 300, 604, 1114, 1117, 1300
BccI CCATC 5 cut(s) 47, 107, 872, 932, 1132
BceAI ACGGC 2 cut(s) 432, 438
BciT130I CCWGG 2 cut(s) 330, 1529
BclI TGATCA 1 cut(s) 925
BcoDI GTCTC 5 cut(s) 188, 350, 442, 1035, 1393
BfaI CTAG 3 cut(s) 47, 458, 509
BfmI CTRYAG 1 cut(s) 1674
BfoI RGCGCY 1 cut(s) 1682
BglII AGATCT 1 cut(s) 965
BisI GCNGC 5 cut(s) 314, 618, 1128, 1131, 1314
BlpI GCTNAGC 2 cut(s) 1119, 1155
BlsI GCNGC 5 cut(s) 315, 619, 1129, 1132, 1315
BmcAI AGTACT 1 cut(s) 1284
Bme1390I CCNGG 2 cut(s) 330, 1529
Bme18I GGWCC 3 cut(s) 326, 498, 1141
BmgT120I GGNCC 3 cut(s) 326, 498, 1141
BmiI GGNNCC 3 cut(s) 466, 499, 1303
BmrFI CCNGG 2 cut(s) 330, 1529
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 6 cut(s) 185, 367, 404, 762, 1330, 1481
BmtI GCTAGC 1 cut(s) 461
BmuI ACTGGG 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 497, 796
BpmI CTGGAG 2 cut(s) 606, 1402
Bpu1102I GCTNAGC 2 cut(s) 1119, 1155
Bpu14I TTCGAA 2 cut(s) 1064, 1558
BpuEI CTTGAG 2 cut(s) 654, 1014
Bsa29I ATCGAT 1 cut(s) 1014
BsaBI GATNNNNATC 1 cut(s) 882
BsaI GGTCTC 2 cut(s) 350, 442
BsaJI CCNNGG 4 cut(s) 229, 773, 1144, 1527
BsaXI ACNNNNNCTCC 2 cut(s) 1508, 1538
Bsc4I CCNNNNNNNGG 2 cut(s) 1150, 1528
Bse1I ACTGG 3 cut(s) 136, 583, 1419
Bse3DI GCAATG 1 cut(s) 1140
Bse8I GATNNNNATC 1 cut(s) 882
BseBI CCWGG 2 cut(s) 330, 1529
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 4 cut(s) 229, 773, 1144, 1527
BseGI GGATG 4 cut(s) 943, 1252, 1518, 1665
BseJI GATNNNNATC 1 cut(s) 882
BseLI CCNNNNNNNGG 2 cut(s) 1150, 1528
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 1 cut(s) 1169
BseNI ACTGG 3 cut(s) 136, 583, 1419
BseXI GCAGC 5 cut(s) 300, 604, 1114, 1117, 1300
Bsh1236I CGCG 1 cut(s) 854
BshFI GGCC 2 cut(s) 69, 1481
BshVI ATCGAT 1 cut(s) 1014
BsiHKAI GWGCWC 1 cut(s) 596
BsiSI CCGG 1 cut(s) 1453
BslFI GGGAC 2 cut(s) 425, 511
BslI CCNNNNNNNGG 2 cut(s) 1150, 1528
BsmAI GTCTC 5 cut(s) 188, 350, 442, 1035, 1393
BsmBI CGTCTC 1 cut(s) 1393
BsmFI GGGAC 2 cut(s) 425, 511
BsmI GAATGC 1 cut(s) 1611
BsnI GGCC 2 cut(s) 69, 1481
Bso31I GGTCTC 2 cut(s) 350, 442
Bsp119I TTCGAA 2 cut(s) 1064, 1558
Bsp1286I GDGCHC 1 cut(s) 596
Bsp1720I GCTNAGC 2 cut(s) 1119, 1155
BspACI CCGC 1 cut(s) 1639
BspANI GGCC 2 cut(s) 69, 1481
BspCNI CTCAG 1 cut(s) 1168
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 1 cut(s) 854
BspLI GGNNCC 3 cut(s) 466, 499, 1303
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 8 cut(s) 459, 472, 589, 632, 878, 1230, 1322, 1507
BspT104I TTCGAA 2 cut(s) 1064, 1558
BspTNI GGTCTC 2 cut(s) 350, 442
BsrDI GCAATG 1 cut(s) 1140
BsrI ACTGG 3 cut(s) 136, 583, 1419
BssECI CCNNGG 4 cut(s) 229, 773, 1144, 1527
BssNAI GTATAC 1 cut(s) 1552
BssSI CACGAG 1 cut(s) 1056
BssT1I CCWWGG 2 cut(s) 229, 773
Bst1107I GTATAC 1 cut(s) 1552
Bst2BI CACGAG 1 cut(s) 1056
Bst2UI CCWGG 2 cut(s) 330, 1529
Bst4CI ACNGT 3 cut(s) 226, 840, 1173
Bst6I CTCTTC 2 cut(s) 353, 779
BstBI TTCGAA 2 cut(s) 1064, 1558
BstC8I GCNNGC 5 cut(s) 455, 459, 701, 1125, 1269
BstDEI CTNAG 3 cut(s) 710, 1119, 1155
BstDSI CCRYGG 1 cut(s) 1144
BstF5I GGATG 4 cut(s) 943, 1252, 1518, 1665
BstFNI CGCG 1 cut(s) 854
BstH2I RGCGCY 1 cut(s) 1682
BstHHI GCGC 2 cut(s) 856, 1681
BstMAI GTCTC 5 cut(s) 188, 350, 442, 1035, 1393
BstMWI GCNNNNNNNGC 5 cut(s) 1124, 1277, 1322, 1478, 1487
BstNI CCWGG 2 cut(s) 330, 1529
BstNSI RCATGY 2 cut(s) 818, 1271
BstSCI CCNGG 2 cut(s) 328, 1527
BstSFI CTRYAG 1 cut(s) 1674
BstUI CGCG 1 cut(s) 854
BstV1I GCAGC 5 cut(s) 300, 604, 1114, 1117, 1300
BstV2I GAAGAC 2 cut(s) 497, 796
BstX2I RGATCY 3 cut(s) 464, 581, 965
BstXI CCANNNNNNTGG 1 cut(s) 131
BstYI RGATCY 3 cut(s) 464, 581, 965
BstZ17I GTATAC 1 cut(s) 1552
Bsu15I ATCGAT 1 cut(s) 1014
BsuRI GGCC 2 cut(s) 69, 1481
BsuTUI ATCGAT 1 cut(s) 1014
BtgI CCRYGG 1 cut(s) 1144
BtsCI GGATG 4 cut(s) 943, 1252, 1518, 1665
BtsI GCAGTG 2 cut(s) 678, 1539
BtsIMutI CAGTG 3 cut(s) 678, 1426, 1539
Cac8I GCNNGC 5 cut(s) 455, 459, 701, 1125, 1269
CfoI GCGC 2 cut(s) 856, 1681
Cfr13I GGNCC 3 cut(s) 326, 498, 1141
ClaI ATCGAT 1 cut(s) 1014
CsiI ACCWGGT 1 cut(s) 328
Csp6I GTAC 2 cut(s) 371, 1283
CviAII CATG 7 cut(s) 43, 213, 621, 628, 815, 1268, 1569
CviQI GTAC 2 cut(s) 371, 1283
DdeI CTNAG 3 cut(s) 710, 1119, 1155
EaeI YGGCCR 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 353, 779
EarI CTCTTC 2 cut(s) 353, 779
Eco130I CCWWGG 2 cut(s) 229, 773
Eco31I GGTCTC 2 cut(s) 350, 442
Eco47I GGWCC 3 cut(s) 326, 498, 1141
Eco47III AGCGCT 1 cut(s) 1680
EcoO109I RGGNCCY 2 cut(s) 326, 498
EcoRII CCWGG 2 cut(s) 328, 1527
EcoT14I CCWWGG 2 cut(s) 229, 773
ErhI CCWWGG 2 cut(s) 229, 773
Esp3I CGTCTC 1 cut(s) 1393
FaeI CATG 7 cut(s) 46, 216, 624, 631, 818, 1271, 1572
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FaqI GGGAC 2 cut(s) 425, 511
FatI CATG 7 cut(s) 42, 212, 620, 627, 814, 1267, 1568
FauNDI CATATG 2 cut(s) 199, 799
FbaI TGATCA 1 cut(s) 925
FblI GTMKAC 1 cut(s) 1551
Fnu4HI GCNGC 5 cut(s) 314, 618, 1128, 1131, 1314
FokI GGATG 4 cut(s) 950, 1259, 1505, 1672
Fsp4HI GCNGC 5 cut(s) 314, 618, 1128, 1131, 1314
FspBI CTAG 3 cut(s) 47, 458, 509
GlaI GCGC 2 cut(s) 855, 1680
GluI GCNGC 5 cut(s) 314, 618, 1128, 1131, 1314
GsuI CTGGAG 2 cut(s) 606, 1402
HaeII RGCGCY 1 cut(s) 1682
HaeIII GGCC 2 cut(s) 69, 1481
HapII CCGG 1 cut(s) 1453
HhaI GCGC 2 cut(s) 856, 1681
Hin1II CATG 7 cut(s) 46, 216, 624, 631, 818, 1271, 1572
Hin6I GCGC 2 cut(s) 854, 1679
HinP1I GCGC 2 cut(s) 854, 1679
HincII GTYRAC 1 cut(s) 1348
HindII GTYRAC 1 cut(s) 1348
HinfI GANTC 6 cut(s) 204, 307, 778, 829, 981, 1061
HpaII CCGG 1 cut(s) 1453
HphI GGTGA 1 cut(s) 893
Hpy166II GTNNAC 6 cut(s) 121, 716, 1348, 1434, 1552, 1708
Hpy188III TCNNGA 5 cut(s) 399, 585, 916, 1058, 1715
Hpy8I GTNNAC 6 cut(s) 121, 716, 1348, 1434, 1552, 1708
HpyAV CCTTC 1 cut(s) 1657
HpyCH4III ACNGT 3 cut(s) 226, 840, 1173
HpyCH4IV ACGT 1 cut(s) 999
HpyF10VI GCNNNNNNNGC 5 cut(s) 1124, 1277, 1322, 1478, 1487
HpyF3I CTNAG 3 cut(s) 710, 1119, 1155
HpySE526I ACGT 1 cut(s) 999
Hsp92II CATG 7 cut(s) 46, 216, 624, 631, 818, 1271, 1572
HspAI GCGC 2 cut(s) 854, 1679
Ksp22I TGATCA 1 cut(s) 925
LmnI GCTCC 2 cut(s) 1421, 1572
Lsp1109I GCAGC 5 cut(s) 300, 604, 1114, 1117, 1300
LweI GCATC 6 cut(s) 185, 367, 404, 762, 1330, 1481
MabI ACCWGGT 1 cut(s) 328
MaeI CTAG 3 cut(s) 47, 458, 509
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 5 cut(s) 92, 260, 521, 1504, 1514
MboII GAAGA 9 cut(s) 232, 340, 497, 796, 801, 1078, 1214, 1375, 1468
MflI RGATCY 3 cut(s) 464, 581, 965
MhlI GDGCHC 1 cut(s) 596
MlsI TGGCCA 1 cut(s) 69
MluNI TGGCCA 1 cut(s) 69
MmeI TCCRAC 2 cut(s) 424, 685
MnlI CCTC 6 cut(s) 254, 366, 678, 1237, 1282, 1532
Mox20I TGGCCA 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 911
MscI TGGCCA 1 cut(s) 69
MseI TTAA 6 cut(s) 51, 158, 575, 962, 1110, 1251
MslI CAYNNNNRTG 2 cut(s) 813, 1616
Msp20I TGGCCA 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 1453
MspR9I CCNGG 2 cut(s) 330, 1529
Mva1269I GAATGC 1 cut(s) 1611
MvaI CCWGG 2 cut(s) 330, 1529
MvnI CGCG 1 cut(s) 854
MwoI GCNNNNNNNGC 5 cut(s) 1124, 1277, 1322, 1478, 1487
NdeI CATATG 2 cut(s) 199, 799
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 7 cut(s) 46, 216, 624, 631, 818, 1271, 1572
NlaIV GGNNCC 3 cut(s) 466, 499, 1303
NmuCI GTSAC 2 cut(s) 92, 521
NspI RCATGY 2 cut(s) 818, 1271
NspV TTCGAA 2 cut(s) 1064, 1558
PaeI GCATGC 1 cut(s) 1271
PcsI WCGNNNNNNNCGW 1 cut(s) 1020
PctI GAATGC 1 cut(s) 1611
PdmI GAANNNNTTC 1 cut(s) 911
PfeI GAWTC 6 cut(s) 204, 307, 778, 829, 981, 1061
PkrI GCNGC 5 cut(s) 315, 619, 1129, 1132, 1315
PpuMI RGGWCCY 2 cut(s) 326, 498
PsiI TTATAA 1 cut(s) 1601
Psp5II RGGWCCY 2 cut(s) 326, 498
Psp6I CCWGG 2 cut(s) 328, 1527
PspGI CCWGG 2 cut(s) 328, 1527
PspN4I GGNNCC 3 cut(s) 466, 499, 1303
PspPI GGNCC 3 cut(s) 326, 498, 1141
PspPPI RGGWCCY 2 cut(s) 326, 498
PsuI RGATCY 3 cut(s) 464, 581, 965
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 2 cut(s) 372, 1284
RsaNI GTAC 2 cut(s) 371, 1283
RseI CAYNNNNRTG 2 cut(s) 813, 1616
SaqAI TTAA 6 cut(s) 51, 158, 575, 962, 1110, 1251
SatI GCNGC 5 cut(s) 314, 618, 1128, 1131, 1314
Sau96I GGNCC 3 cut(s) 326, 498, 1141
ScaI AGTACT 1 cut(s) 1284
ScrFI CCNGG 2 cut(s) 330, 1529
SduI GDGCHC 1 cut(s) 596
SexAI ACCWGGT 1 cut(s) 328
SfaNI GCATC 6 cut(s) 185, 367, 404, 762, 1330, 1481
SfcI CTRYAG 1 cut(s) 1674
SfuI TTCGAA 2 cut(s) 1064, 1558
SinI GGWCC 3 cut(s) 326, 498, 1141
SmiMI CAYNNNNRTG 2 cut(s) 813, 1616
SmlI CTYRAG 2 cut(s) 669, 1029
SmoI CTYRAG 2 cut(s) 669, 1029
SphI GCATGC 1 cut(s) 1271
SsiI CCGC 1 cut(s) 1639
SspMI CTAG 3 cut(s) 47, 458, 509
StyD4I CCNGG 2 cut(s) 328, 1527
StyI CCWWGG 2 cut(s) 229, 773
TaaI ACNGT 3 cut(s) 226, 840, 1173
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 3 cut(s) 1014, 1064, 1558
TatI WGTACW 1 cut(s) 1282
TfiI GAWTC 6 cut(s) 204, 307, 778, 829, 981, 1061
Tru1I TTAA 6 cut(s) 51, 158, 575, 962, 1110, 1251
Tru9I TTAA 6 cut(s) 51, 158, 575, 962, 1110, 1251
TscAI CASTG 3 cut(s) 685, 1426, 1539
TseFI GTSAC 2 cut(s) 92, 521
TseI GCWGC 5 cut(s) 313, 617, 1127, 1130, 1313
Tsp45I GTSAC 2 cut(s) 92, 521
TspDTI ATGAA 4 cut(s) 564, 1032, 1095, 1181
TspGWI ACGGA 2 cut(s) 430, 1133
TspRI CASTG 3 cut(s) 685, 1426, 1539
VpaK11BI GGWCC 3 cut(s) 326, 498, 1141
XapI RAATTY 5 cut(s) 145, 318, 532, 1082, 1095
XceI RCATGY 2 cut(s) 818, 1271
XmiI GTMKAC 1 cut(s) 1551
XmnI GAANNNNTTC 1 cut(s) 911
XspI CTAG 3 cut(s) 47, 458, 509
ZrmI AGTACT 1 cut(s) 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.