Rmu_sc0001643.1_g000033

Sodium/calcium exchanger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001643.1
OG0015661
Physical Location & Seq
Reverse (-)
167905 .. 171341
3437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001643.1_g000033.1.cds

Sequence Viewer

Length: 1695 bp
atgctagttaatctaaccagctttggccaaagtcgttctattgctacaagtgacttcaatttagtgtctgatggagtaaaccacactactggggataaacccaattttctttggttaaagcatagcttgtttgcatccgatagctgtgatgagacatatgggttcttgccatgcaccaacacagtccttggtaatatcttcctcattatcgtctatggttacttgatgttcctttcagccaagttattgtcagctggaagtgagattctgctgcaaattttaggacctggtatcatagggggtctcttcttgcctctgttgagtgctgtacctgatgccaccattatccttgcatcaggactttctggggacacggcaacggcacaaagtcaggtctccgttggaatgggcttgctagctggatccaatgtaatgcttctcacaatcctatgggggacctgtcttctagttggcaaatgtgaccttgaaaattcagttgcagttgatgaaaaagatacaaagcgttttagcttaactggatctggagtgagcacagatatttggacaagctatgctgcatggatcatggttatctccattattccttttgtaatagtccaactacctcaagttttcactgcgactttggatagtagcttgccaattctaagttcactcattgttgcaatctgttttgtgatcgcttatagcatctatcagatttttcagccttggattcagaagagaagacttgcatatgcaaagcataagcatgtgatgtcagagattctaaaacagttgaaaacaaacgcgcttggaaaattgcttacagatgatggtgatcctaacaaagttgttatagagaagttattcaggacacttgatcagaactcggatggttatttgaacaccacagacttaagatctttgataataggaatccaatttgatgatgacgtagatataaatatcgatgaagccatttctcaagtgatgagagactttgacacaactcgtgattcgaagattgatgttgatgaattcttcaaaggaatttcaaggtggcttaacaaggctaagcgtgctgcaatgatggaccgtggaaaggctccgctatcaatgaaactgttagatgattttgataagcaaacaaaggaagaacaagatcggtatggggatcagattgatgagattgtggaggatgttaaaaatgcaaactggcatgcctcaaaagcagtactgatgttgcttctgggaaccattgttgctgctgtggttgctgatccccttgttgatgctgttgacaacttctcaacagctacaagtattcctacattcttcgtatcatttgttattctgccttttgttagctccagtgagattgtgtccactctaatttttgtcagccggaagaagcagagaacagcatcattggcctactctgcgatatatggatcagtaacaatgagtaacatcctctccctggcagtgttcttggctcttgtatacattcgaaatctgacatggagcttctcgtctgaagttcttgtcattataattgtctgcattctaatgggagtggttgctggtttccgcaccaccttccctgtttggatgtcttttggggctgtagcgctctatccactttctctgttgctggtttacattcttgaatatgtttttgggtggtcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

564

Amino Acids

61.99

Weight (kDa)

4.99

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015661)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g26990 FvH4_6g26990 FvH4_6g26990 FvH4_6g26990
malus_domestica MD17G1233800.v1.1
prunus_persica Prupe.3G125200_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0128851
rosa_laevigata RLG00000019033
rosa_multiflora Rmu_sc0001643.1_g000033
rosa_roxburghii Rroxscaffold_2G00115170
rosa_rugosa Rorug02G0281800 Rorug02G0281800
rosa_samantha Rh2AG334300 Rh2CG321200 Rh2DG359000
rosa_wichuraiana Rw2G027040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1556
AccI GTMKAC 1 cut(s) 1506
AccII CGCG 1 cut(s) 812
AciI CCGC 2 cut(s) 1112, 1594
AclWI GGATC 8 cut(s) 417, 430, 547, 590, 836, 1185, 1277, 1462
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 4 cut(s) 276, 490, 1040, 1053
AcuI CTGAAG 1 cut(s) 1560
AfaI GTAC 2 cut(s) 330, 1239
AfeI AGCGCT 1 cut(s) 1635
AfiI CCNNNNNNNGG 2 cut(s) 1105, 1483
AflII CTTAAG 1 cut(s) 919
AgsI TTSAA 7 cut(s) 58, 488, 802, 907, 1048, 1059, 1673
AjnI CCWGG 2 cut(s) 286, 1482
AjuI GAANNNNNNNTTGG 3 cut(s) 655, 687, 1665
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 4 cut(s) 146, 308, 400, 993
AlwI GGATC 8 cut(s) 417, 430, 547, 590, 836, 1185, 1277, 1462
Aor51HI AGCGCT 1 cut(s) 1635
AoxI GGCC 2 cut(s) 25, 1434
ApeKI GCWGC 4 cut(s) 271, 575, 1085, 1268
ApoI RAATTY 4 cut(s) 276, 490, 1040, 1053
Asp700I GAANNNNTTC 1 cut(s) 869
AspLEI GCGC 2 cut(s) 814, 1636
AspS9I GGNCC 3 cut(s) 284, 456, 1096
AsuHPI GGTGA 1 cut(s) 851
AsuII TTCGAA 2 cut(s) 1022, 1513
AsuNHI GCTAGC 1 cut(s) 415
AvaII GGWCC 3 cut(s) 284, 456, 1096
BalI TGGCCA 1 cut(s) 27
BamHI GGATCC 1 cut(s) 422
BauI CACGAG 1 cut(s) 1014
BbsI GAAGAC 2 cut(s) 455, 754
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 258, 562, 1072, 1255
BccI CCATC 4 cut(s) 65, 830, 890, 1087
BceAI ACGGC 2 cut(s) 390, 396
BciT130I CCWGG 2 cut(s) 288, 1484
BclI TGATCA 1 cut(s) 883
BcoDI GTCTC 4 cut(s) 146, 308, 400, 993
BfaI CTAG 3 cut(s) 5, 416, 467
BfmI CTRYAG 1 cut(s) 1629
BfoI RGCGCY 1 cut(s) 1637
BfrI CTTAAG 1 cut(s) 919
BglII AGATCT 1 cut(s) 923
BisI GCNGC 4 cut(s) 272, 576, 1086, 1269
BlpI GCTNAGC 1 cut(s) 1077
BlsI GCNGC 4 cut(s) 273, 577, 1087, 1270
BmcAI AGTACT 1 cut(s) 1239
Bme1390I CCNGG 2 cut(s) 288, 1484
Bme18I GGWCC 3 cut(s) 284, 456, 1096
BmgT120I GGNCC 3 cut(s) 284, 456, 1096
BmiI GGNNCC 4 cut(s) 424, 457, 1110, 1258
BmrFI CCNGG 2 cut(s) 288, 1484
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 6 cut(s) 143, 325, 362, 720, 1285, 1436
BmtI GCTAGC 1 cut(s) 419
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 455, 754
BpmI CTGGAG 2 cut(s) 564, 1357
Bpu1102I GCTNAGC 1 cut(s) 1077
Bpu14I TTCGAA 2 cut(s) 1022, 1513
BpuEI CTTGAG 2 cut(s) 612, 972
Bsa29I ATCGAT 1 cut(s) 972
BsaBI GATNNNNATC 1 cut(s) 840
BsaI GGTCTC 2 cut(s) 308, 400
BsaJI CCNNGG 4 cut(s) 187, 731, 1099, 1482
BsaXI ACNNNNNCTCC 2 cut(s) 1463, 1493
Bsc4I CCNNNNNNNGG 2 cut(s) 1105, 1483
Bse1I ACTGG 4 cut(s) 94, 541, 1223, 1374
Bse3DI GCAATG 1 cut(s) 1095
Bse8I GATNNNNATC 1 cut(s) 840
BseBI CCWGG 2 cut(s) 288, 1484
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 4 cut(s) 187, 731, 1099, 1482
BseGI GGATG 5 cut(s) 134, 901, 1207, 1473, 1620
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 2 cut(s) 1105, 1483
BseMI GCAATG 1 cut(s) 1095
BseNI ACTGG 4 cut(s) 94, 541, 1223, 1374
BseXI GCAGC 4 cut(s) 258, 562, 1072, 1255
Bsh1236I CGCG 1 cut(s) 812
BshFI GGCC 2 cut(s) 27, 1436
BshVI ATCGAT 1 cut(s) 972
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 1 cut(s) 1408
BslFI GGGAC 2 cut(s) 383, 469
BslI CCNNNNNNNGG 2 cut(s) 1105, 1483
BsmAI GTCTC 4 cut(s) 146, 308, 400, 993
BsmFI GGGAC 2 cut(s) 383, 469
BsmI GAATGC 1 cut(s) 1566
BsnI GGCC 2 cut(s) 27, 1436
Bso31I GGTCTC 2 cut(s) 308, 400
Bsp119I TTCGAA 2 cut(s) 1022, 1513
Bsp1286I GDGCHC 1 cut(s) 554
Bsp1720I GCTNAGC 1 cut(s) 1077
BspACI CCGC 2 cut(s) 1112, 1594
BspANI GGCC 2 cut(s) 27, 1436
BspDI ATCGAT 1 cut(s) 972
BspFNI CGCG 1 cut(s) 812
BspLI GGNNCC 4 cut(s) 424, 457, 1110, 1258
BspOI GCTAGC 1 cut(s) 419
BspPI GGATC 8 cut(s) 417, 430, 547, 590, 836, 1185, 1277, 1462
BspT104I TTCGAA 2 cut(s) 1022, 1513
BspTI CTTAAG 1 cut(s) 919
BspTNI GGTCTC 2 cut(s) 308, 400
BsrDI GCAATG 1 cut(s) 1095
BsrI ACTGG 4 cut(s) 94, 541, 1223, 1374
BssECI CCNNGG 4 cut(s) 187, 731, 1099, 1482
BssNAI GTATAC 1 cut(s) 1507
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 2 cut(s) 187, 731
Bst1107I GTATAC 1 cut(s) 1507
Bst2BI CACGAG 1 cut(s) 1014
Bst2UI CCWGG 2 cut(s) 288, 1484
Bst4CI ACNGT 4 cut(s) 184, 798, 1100, 1128
Bst6I CTCTTC 2 cut(s) 311, 737
BstAFI CTTAAG 1 cut(s) 919
BstBI TTCGAA 2 cut(s) 1022, 1513
BstC8I GCNNGC 5 cut(s) 413, 417, 659, 1083, 1224
BstDEI CTNAG 2 cut(s) 668, 1077
BstDSI CCRYGG 1 cut(s) 1099
BstF5I GGATG 5 cut(s) 134, 901, 1207, 1473, 1620
BstFNI CGCG 1 cut(s) 812
BstH2I RGCGCY 1 cut(s) 1637
BstHHI GCGC 2 cut(s) 814, 1636
BstMAI GTCTC 4 cut(s) 146, 308, 400, 993
BstMWI GCNNNNNNNGC 5 cut(s) 1082, 1232, 1277, 1433, 1442
BstNI CCWGG 2 cut(s) 288, 1484
BstNSI RCATGY 2 cut(s) 776, 1226
BstSCI CCNGG 2 cut(s) 286, 1482
BstSFI CTRYAG 1 cut(s) 1629
BstUI CGCG 1 cut(s) 812
BstV1I GCAGC 4 cut(s) 258, 562, 1072, 1255
BstV2I GAAGAC 2 cut(s) 455, 754
BstX2I RGATCY 3 cut(s) 422, 539, 923
BstXI CCANNNNNNTGG 1 cut(s) 89
BstYI RGATCY 3 cut(s) 422, 539, 923
BstZ17I GTATAC 1 cut(s) 1507
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 2 cut(s) 27, 1436
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 1099
BtsCI GGATG 5 cut(s) 134, 901, 1207, 1473, 1620
BtsI GCAGTG 2 cut(s) 636, 1494
BtsIMutI CAGTG 3 cut(s) 636, 1381, 1494
Cac8I GCNNGC 5 cut(s) 413, 417, 659, 1083, 1224
CfoI GCGC 2 cut(s) 814, 1636
Cfr13I GGNCC 3 cut(s) 284, 456, 1096
ClaI ATCGAT 1 cut(s) 972
CsiI ACCWGGT 1 cut(s) 286
Csp6I GTAC 2 cut(s) 329, 1238
CviAII CATG 6 cut(s) 171, 579, 586, 773, 1223, 1524
CviQI GTAC 2 cut(s) 329, 1238
DdeI CTNAG 2 cut(s) 668, 1077
EaeI YGGCCR 1 cut(s) 25
Eam1104I CTCTTC 2 cut(s) 311, 737
EarI CTCTTC 2 cut(s) 311, 737
Eco130I CCWWGG 2 cut(s) 187, 731
Eco31I GGTCTC 2 cut(s) 308, 400
Eco47I GGWCC 3 cut(s) 284, 456, 1096
Eco47III AGCGCT 1 cut(s) 1635
Eco57I CTGAAG 1 cut(s) 1560
EcoO109I RGGNCCY 2 cut(s) 284, 456
EcoRI GAATTC 1 cut(s) 1040
EcoRII CCWGG 2 cut(s) 286, 1482
EcoT14I CCWWGG 2 cut(s) 187, 731
ErhI CCWWGG 2 cut(s) 187, 731
FaeI CATG 6 cut(s) 174, 582, 589, 776, 1226, 1527
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 2 cut(s) 383, 469
FatI CATG 6 cut(s) 170, 578, 585, 772, 1222, 1523
FauNDI CATATG 2 cut(s) 157, 757
FbaI TGATCA 1 cut(s) 883
FblI GTMKAC 1 cut(s) 1506
Fnu4HI GCNGC 4 cut(s) 272, 576, 1086, 1269
FokI GGATG 5 cut(s) 121, 908, 1214, 1460, 1627
Fsp4HI GCNGC 4 cut(s) 272, 576, 1086, 1269
FspBI CTAG 3 cut(s) 5, 416, 467
GlaI GCGC 2 cut(s) 813, 1635
GluI GCNGC 4 cut(s) 272, 576, 1086, 1269
GsuI CTGGAG 2 cut(s) 564, 1357
HaeII RGCGCY 1 cut(s) 1637
HaeIII GGCC 2 cut(s) 27, 1436
HapII CCGG 1 cut(s) 1408
HhaI GCGC 2 cut(s) 814, 1636
Hin1II CATG 6 cut(s) 174, 582, 589, 776, 1226, 1527
Hin6I GCGC 2 cut(s) 812, 1634
HinP1I GCGC 2 cut(s) 812, 1634
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HinfI GANTC 5 cut(s) 265, 736, 787, 939, 1019
HpaII CCGG 1 cut(s) 1408
HphI GGTGA 1 cut(s) 851
Hpy166II GTNNAC 6 cut(s) 79, 674, 1303, 1389, 1507, 1663
Hpy188III TCNNGA 5 cut(s) 357, 543, 874, 1016, 1670
Hpy8I GTNNAC 6 cut(s) 79, 674, 1303, 1389, 1507, 1663
HpyAV CCTTC 1 cut(s) 1612
HpyCH4III ACNGT 4 cut(s) 184, 798, 1100, 1128
HpyCH4IV ACGT 1 cut(s) 957
HpyF10VI GCNNNNNNNGC 5 cut(s) 1082, 1232, 1277, 1433, 1442
HpyF3I CTNAG 2 cut(s) 668, 1077
HpySE526I ACGT 1 cut(s) 957
Hsp92II CATG 6 cut(s) 174, 582, 589, 776, 1226, 1527
HspAI GCGC 2 cut(s) 812, 1634
Ksp22I TGATCA 1 cut(s) 883
LmnI GCTCC 3 cut(s) 1114, 1376, 1527
Lsp1109I GCAGC 4 cut(s) 258, 562, 1072, 1255
LweI GCATC 6 cut(s) 143, 325, 362, 720, 1285, 1436
MabI ACCWGGT 1 cut(s) 286
MaeI CTAG 3 cut(s) 5, 416, 467
MaeII ACGT 1 cut(s) 957
MaeIII GTNAC 5 cut(s) 50, 218, 479, 1459, 1469
MflI RGATCY 3 cut(s) 422, 539, 923
MhlI GDGCHC 1 cut(s) 554
MlsI TGGCCA 1 cut(s) 27
MluNI TGGCCA 1 cut(s) 27
MmeI TCCRAC 2 cut(s) 382, 643
MnlI CCTC 6 cut(s) 212, 324, 636, 1192, 1237, 1487
Mox20I TGGCCA 1 cut(s) 27
MroXI GAANNNNTTC 1 cut(s) 869
MscI TGGCCA 1 cut(s) 27
MseI TTAA 6 cut(s) 9, 116, 533, 920, 1068, 1206
MslI CAYNNNNRTG 2 cut(s) 771, 1571
Msp20I TGGCCA 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 254
MspCI CTTAAG 1 cut(s) 919
MspI CCGG 1 cut(s) 1408
MspR9I CCNGG 2 cut(s) 288, 1484
Mva1269I GAATGC 1 cut(s) 1566
MvaI CCWGG 2 cut(s) 288, 1484
MvnI CGCG 1 cut(s) 812
MwoI GCNNNNNNNGC 5 cut(s) 1082, 1232, 1277, 1433, 1442
NdeI CATATG 2 cut(s) 157, 757
NheI GCTAGC 1 cut(s) 415
NlaIII CATG 6 cut(s) 174, 582, 589, 776, 1226, 1527
NlaIV GGNNCC 4 cut(s) 424, 457, 1110, 1258
NmuCI GTSAC 2 cut(s) 50, 479
NspI RCATGY 2 cut(s) 776, 1226
NspV TTCGAA 2 cut(s) 1022, 1513
PaeI GCATGC 1 cut(s) 1226
PctI GAATGC 1 cut(s) 1566
PdmI GAANNNNTTC 1 cut(s) 869
PfeI GAWTC 5 cut(s) 265, 736, 787, 939, 1019
PkrI GCNGC 4 cut(s) 273, 577, 1087, 1270
PpuMI RGGWCCY 2 cut(s) 284, 456
PsiI TTATAA 1 cut(s) 1556
Psp5II RGGWCCY 2 cut(s) 284, 456
Psp6I CCWGG 2 cut(s) 286, 1482
PspGI CCWGG 2 cut(s) 286, 1482
PspN4I GGNNCC 4 cut(s) 424, 457, 1110, 1258
PspPI GGNCC 3 cut(s) 284, 456, 1096
PspPPI RGGWCCY 2 cut(s) 284, 456
PsuI RGATCY 3 cut(s) 422, 539, 923
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 2 cut(s) 330, 1239
RsaNI GTAC 2 cut(s) 329, 1238
RseI CAYNNNNRTG 2 cut(s) 771, 1571
SaqAI TTAA 6 cut(s) 9, 116, 533, 920, 1068, 1206
SatI GCNGC 4 cut(s) 272, 576, 1086, 1269
Sau96I GGNCC 3 cut(s) 284, 456, 1096
ScaI AGTACT 1 cut(s) 1239
ScrFI CCNGG 2 cut(s) 288, 1484
SduI GDGCHC 1 cut(s) 554
SexAI ACCWGGT 1 cut(s) 286
SfaNI GCATC 6 cut(s) 143, 325, 362, 720, 1285, 1436
SfcI CTRYAG 1 cut(s) 1629
SfuI TTCGAA 2 cut(s) 1022, 1513
SinI GGWCC 3 cut(s) 284, 456, 1096
SmiMI CAYNNNNRTG 2 cut(s) 771, 1571
SmlI CTYRAG 3 cut(s) 627, 919, 987
SmoI CTYRAG 3 cut(s) 627, 919, 987
SphI GCATGC 1 cut(s) 1226
SsiI CCGC 2 cut(s) 1112, 1594
SspMI CTAG 3 cut(s) 5, 416, 467
StyD4I CCNGG 2 cut(s) 286, 1482
StyI CCWWGG 2 cut(s) 187, 731
TaaI ACNGT 4 cut(s) 184, 798, 1100, 1128
TaiI ACGT 1 cut(s) 960
TaqI TCGA 3 cut(s) 972, 1022, 1513
TatI WGTACW 1 cut(s) 1237
TfiI GAWTC 5 cut(s) 265, 736, 787, 939, 1019
Tru1I TTAA 6 cut(s) 9, 116, 533, 920, 1068, 1206
Tru9I TTAA 6 cut(s) 9, 116, 533, 920, 1068, 1206
TscAI CASTG 3 cut(s) 643, 1381, 1494
TseFI GTSAC 2 cut(s) 50, 479
TseI GCWGC 4 cut(s) 271, 575, 1085, 1268
Tsp45I GTSAC 2 cut(s) 50, 479
TspDTI ATGAA 4 cut(s) 522, 990, 1053, 1136
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 3 cut(s) 643, 1381, 1494
Vha464I CTTAAG 1 cut(s) 919
VpaK11BI GGWCC 3 cut(s) 284, 456, 1096
XapI RAATTY 4 cut(s) 276, 490, 1040, 1053
XceI RCATGY 2 cut(s) 776, 1226
XmiI GTMKAC 1 cut(s) 1506
XmnI GAANNNNTTC 1 cut(s) 869
XspI CTAG 3 cut(s) 5, 416, 467
ZrmI AGTACT 1 cut(s) 1239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.