Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0013812

Physical Location & Seq

Genomic Coordinates Forward (+)

32473295 .. 32476103

Gene Structure 2809 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1264100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 657 bp

ATGGCGGGGTACAAAGGTGACGATGAGTACGATTACTTGTTCAAGCTGGTATTGATTGGGGATTCTGGGGTGGGAAAGTCGAACCTGCTTTCCAGGTTCACCAGGAACGAGTTTAATTTGGAGTCCAAGTCTACCATCGGTGTCGAGTTCGCCACCAAGACTTTGACCGTTGATTCCAAGCTCATCAAAGCTCAGATTTGGGACACTGCTGGCCAAGAAAGGTACCGTGCCATTACAAGTGCATACTACCGAGGGGCTGTTGGTGCTTTGCTAGTGTATGATGTCACTCGGCATGCCACATTTGAGAATGTAGCAAGGTGGTTGAAGGAACTGAGGAACCATACAGACCCCAACATTGTAGTGATGCTCATCGGCAACAAATCAGATCTTCGCCATCTGGTGGCTGTCTCAAAAGAGGATGGTAAATCTTTTGCCGAAAGAGAGTCCCTCTACTTCATGGAAACTTCTGCGTTGGAAGCAACCAACGTAGAAAGTGCATTTACCCAAGTCTTGACTCAAATATACCGGGTGGTAAGCAAGAGGGCAGTCGAGGCAGGCAACAATGGAAGCGCTTCTGCTGTTCCGTCTAAAGGACAGACCATAAATGTCAAAGATGATGGTTCTGTTTTCAAGAGAATTGGATGCTGCTCAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009987 GO:0010033 GO:0012505 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0031410 GO:0031982 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0035639 GO:0036094 GO:0042221 GO:0042546 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0071554 GO:0071840 GO:0071944 GO:0097159 GO:0097367 GO:0097708 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972

Pfam Domains

Protein Families

Ras

Protein Analysis

219

Amino Acids

24.13

Weight (kDa)

8.43

Isoelectric Point (pI)

20.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	14 - 170	9.7e-13	ADP-ribosylation factor family
Ras	PF00071	15 - 175	5.6e-63	Ras family
Roc	PF08477	15 - 129	5.2e-35	Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1	PF01926	16 - 121	7.7e-06	50S ribosome-binding GTPase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0013812)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06400
fragaria_vesca	FvH4_6g27750
malus_domestica	MD09G1235000.v1.1 MD17G1264100.v1.1
prunus_persica	Prupe.3G113200_v2.0.a1
pyrus_communis	pycom09g15320 pycom17g26390
rosa_chinensis	RchiOBHm_Chr2g0130911
rosa_laevigata	RLG00000019158
rosa_multiflora	Rmu_sc0003629.1_g000032
rosa_roxburghii	Rroxscaffold_2G00113450
rosa_rugosa	Rorug02G0294000
rosa_samantha	Rh2AG345300 Rh2BG353700 Rh2CG332300 Rh2DG371800
rosa_wichuraiana	Rw2G028070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	93
Acc65I	GGTACC	1 cut(s)	222
AccB1I	GGYRCC	1 cut(s)	222
AccB7I	CCANNNNNTGG	1 cut(s)	400
AccI	GTMKAC	1 cut(s)	131
AciI	CCGC	1 cut(s)	5
AcoI	YGGCCR	1 cut(s)	211
AfaI	GTAC	3 cut(s)	11, 29, 224
AfeI	AGCGCT	1 cut(s)	571
AfiI	CCNNNNNNNGG	2 cut(s)	400, 590
AgsI	TTSAA	3 cut(s)	43, 325, 631
AjnI	CCWGG	2 cut(s)	92, 101
AloI	GAACNNNNNNTCC	2 cut(s)	74, 106
AluBI	AGCT	3 cut(s)	46, 181, 191
AluI	AGCT	3 cut(s)	46, 181, 191
Alw26I	GTCTC	1 cut(s)	412
Aor51HI	AGCGCT	1 cut(s)	571
AoxI	GGCC	1 cut(s)	211
ApeKI	GCWGC	1 cut(s)	645
Asp700I	GAANNNNTTC	1 cut(s)	571
Asp718I	GGTACC	1 cut(s)	222
AspLEI	GCGC	1 cut(s)	572
AsuC2I	CCSGG	1 cut(s)	527
AsuHPI	GGTGA	2 cut(s)	29, 91
BaeI	ACNNNNGTAYC	1 cut(s)	34
BalI	TGGCCA	1 cut(s)	213
BanI	GGYRCC	1 cut(s)	222
BbvI	GCAGC	1 cut(s)	632
BccI	CCATC	4 cut(s)	143, 402, 413, 611
BciT130I	CCWGG	2 cut(s)	94, 103
BcnI	CCSGG	1 cut(s)	527
BcoDI	GTCTC	1 cut(s)	412
BfaI	CTAG	2 cut(s)	272, 655
BfoI	RGCGCY	1 cut(s)	573
BfuAI	ACCTGC	1 cut(s)	93
BglII	AGATCT	1 cut(s)	385
BisI	GCNGC	1 cut(s)	646
BlsI	GCNGC	1 cut(s)	647
Bme1390I	CCNGG	3 cut(s)	94, 103, 527
BmiI	GGNNCC	2 cut(s)	224, 338
BmrFI	CCNGG	3 cut(s)	94, 103, 527
BmsI	GCATC	2 cut(s)	354, 632
BplI	GAGNNNNNCTC	2 cut(s)	432, 464
BpuMI	CCSGG	1 cut(s)	527
BsaBI	GATNNNNATC	1 cut(s)	368
BsaJI	CCNNGG	1 cut(s)	250
BsaXI	ACNNNNNCTCC	2 cut(s)	113, 143
Bsc4I	CCNNNNNNNGG	2 cut(s)	400, 590
Bse8I	GATNNNNATC	1 cut(s)	368
BseBI	CCWGG	2 cut(s)	94, 103
BseDI	CCNNGG	1 cut(s)	250
BseGI	GGATG	2 cut(s)	424, 647
BseJI	GATNNNNATC	1 cut(s)	368
BseLI	CCNNNNNNNGG	2 cut(s)	400, 590
BseMII	CTCAG	2 cut(s)	206, 323
BseXI	GCAGC	1 cut(s)	632
BshFI	GGCC	1 cut(s)	213
BshNI	GGYRCC	1 cut(s)	222
BsiSI	CCGG	1 cut(s)	526
BslFI	GGGAC	2 cut(s)	215, 430
BslI	CCNNNNNNNGG	2 cut(s)	400, 590
BsmAI	GTCTC	1 cut(s)	412
BsmFI	GGGAC	2 cut(s)	215, 430
BsnI	GGCC	1 cut(s)	213
Bsp143I	GATC	1 cut(s)	385
BspACI	CCGC	1 cut(s)	5
BspANI	GGCC	1 cut(s)	213
BspCNI	CTCAG	2 cut(s)	205, 324
BspLI	GGNNCC	2 cut(s)	224, 338
BspMI	ACCTGC	1 cut(s)	93
BspT107I	GGYRCC	1 cut(s)	222
BssECI	CCNNGG	1 cut(s)	250
BssMI	GATC	1 cut(s)	385
Bst2UI	CCWGG	2 cut(s)	94, 103
Bst4CI	ACNGT	2 cut(s)	169, 227
BstC8I	GCNNGC	3 cut(s)	211, 294, 556
BstDEI	CTNAG	2 cut(s)	192, 332
BstF5I	GGATG	2 cut(s)	424, 647
BstH2I	RGCGCY	1 cut(s)	573
BstHHI	GCGC	1 cut(s)	572
BstKTI	GATC	1 cut(s)	388
BstMAI	GTCTC	1 cut(s)	412
BstMBI	GATC	1 cut(s)	385
BstMWI	GCNNNNNNNGC	3 cut(s)	263, 476, 551
BstNI	CCWGG	2 cut(s)	94, 103
BstNSI	RCATGY	1 cut(s)	296
BstSCI	CCNGG	3 cut(s)	92, 101, 525
BstV1I	GCAGC	1 cut(s)	632
BstX2I	RGATCY	1 cut(s)	385
BstYI	RGATCY	1 cut(s)	385
BsuRI	GGCC	1 cut(s)	213
BtsCI	GGATG	2 cut(s)	424, 647
BtsI	GCAGTG	1 cut(s)	204
BtsIMutI	CAGTG	1 cut(s)	204
BveI	ACCTGC	1 cut(s)	93
Cac8I	GCNNGC	3 cut(s)	211, 294, 556
CfoI	GCGC	1 cut(s)	572
Csp6I	GTAC	3 cut(s)	10, 28, 223
CviAII	CATG	2 cut(s)	293, 457
CviJI	RGCY	6 cut(s)	46, 181, 191, 213, 257, 404
CviKI_1	RGCY	6 cut(s)	46, 181, 191, 213, 257, 404
CviQI	GTAC	3 cut(s)	10, 28, 223
DdeI	CTNAG	2 cut(s)	192, 332
DpnI	GATC	1 cut(s)	387
DpnII	GATC	1 cut(s)	385
EaeI	YGGCCR	1 cut(s)	211
Eco47III	AGCGCT	1 cut(s)	571
EcoRII	CCWGG	2 cut(s)	92, 101
FaeI	CATG	2 cut(s)	296, 460
FaiI	YATR	7 cut(s)	244, 279, 294, 342, 458, 523, 602
FaqI	GGGAC	2 cut(s)	215, 430
FatI	CATG	2 cut(s)	292, 456
FblI	GTMKAC	1 cut(s)	131
Fnu4HI	GCNGC	1 cut(s)	646
FokI	GGATG	1 cut(s)	431
Fsp4HI	GCNGC	1 cut(s)	646
FspBI	CTAG	2 cut(s)	272, 655
GlaI	GCGC	1 cut(s)	571
GluI	GCNGC	1 cut(s)	646
HaeII	RGCGCY	1 cut(s)	573
HaeIII	GGCC	1 cut(s)	213
HapII	CCGG	1 cut(s)	526
HhaI	GCGC	1 cut(s)	572
Hin1II	CATG	2 cut(s)	296, 460
Hin6I	GCGC	1 cut(s)	570
HinP1I	GCGC	1 cut(s)	570
HinfI	GANTC	5 cut(s)	62, 122, 173, 443, 514
HpaII	CCGG	1 cut(s)	526
HphI	GGTGA	2 cut(s)	29, 91
Hpy166II	GTNNAC	2 cut(s)	99, 132
Hpy188I	TCNGA	2 cut(s)	195, 385
Hpy188III	TCNNGA	2 cut(s)	511, 631
Hpy8I	GTNNAC	2 cut(s)	99, 132
HpyAV	CCTTC	1 cut(s)	319
HpyCH4III	ACNGT	2 cut(s)	169, 227
HpyCH4IV	ACGT	1 cut(s)	486
HpyCH4V	TGCA	2 cut(s)	242, 497
HpyF10VI	GCNNNNNNNGC	3 cut(s)	263, 476, 551
HpyF3I	CTNAG	2 cut(s)	192, 332
HpySE526I	ACGT	1 cut(s)	486
Hsp92II	CATG	2 cut(s)	296, 460
HspAI	GCGC	1 cut(s)	570
KpnI	GGTACC	1 cut(s)	226
Kzo9I	GATC	1 cut(s)	385
Lsp1109I	GCAGC	1 cut(s)	632
LweI	GCATC	2 cut(s)	354, 632
MaeI	CTAG	2 cut(s)	272, 655
MaeII	ACGT	1 cut(s)	486
MaeIII	GTNAC	2 cut(s)	17, 283
MalI	GATC	1 cut(s)	387
MboI	GATC	1 cut(s)	385
MboII	GAAGA	1 cut(s)	380
MflI	RGATCY	1 cut(s)	385
MlsI	TGGCCA	1 cut(s)	213
MluCI	AATT	2 cut(s)	115, 636
MluNI	TGGCCA	1 cut(s)	213
MlyI	GAGTC	3 cut(s)	131, 452, 508
MmeI	TCCRAC	1 cut(s)	453
MnlI	CCTC	6 cut(s)	245, 327, 409, 458, 534, 544
Mox20I	TGGCCA	1 cut(s)	213
MroXI	GAANNNNTTC	1 cut(s)	571
MscI	TGGCCA	1 cut(s)	213
MseI	TTAA	1 cut(s)	114
MslI	CAYNNNNRTG	1 cut(s)	359
Msp20I	TGGCCA	1 cut(s)	213
MspI	CCGG	1 cut(s)	526
MspR9I	CCNGG	3 cut(s)	94, 103, 527
MvaI	CCWGG	2 cut(s)	94, 103
MwoI	GCNNNNNNNGC	3 cut(s)	263, 476, 551
NciI	CCSGG	1 cut(s)	527
NdeII	GATC	1 cut(s)	385
NlaIII	CATG	2 cut(s)	296, 460
NlaIV	GGNNCC	2 cut(s)	224, 338
NmeAIII	GCCGAG	1 cut(s)	268
NmuCI	GTSAC	2 cut(s)	17, 283
NspI	RCATGY	1 cut(s)	296
PaeI	GCATGC	1 cut(s)	296
PcsI	WCGNNNNNNNCGW	1 cut(s)	27
PdmI	GAANNNNTTC	1 cut(s)	571
PfeI	GAWTC	2 cut(s)	62, 173
PflMI	CCANNNNNTGG	1 cut(s)	400
PkrI	GCNGC	1 cut(s)	647
PleI	GAGTC	3 cut(s)	130, 451, 508
PpsI	GAGTC	3 cut(s)	130, 451, 508
Psp6I	CCWGG	2 cut(s)	92, 101
PspGI	CCWGG	2 cut(s)	92, 101
PspN4I	GGNNCC	2 cut(s)	224, 338
PsuI	RGATCY	1 cut(s)	385
RsaI	GTAC	3 cut(s)	11, 29, 224
RsaNI	GTAC	3 cut(s)	10, 28, 223
RseI	CAYNNNNRTG	1 cut(s)	359
SaqAI	TTAA	1 cut(s)	114
SatI	GCNGC	1 cut(s)	646
Sau3AI	GATC	1 cut(s)	385
SchI	GAGTC	3 cut(s)	131, 452, 508
ScrFI	CCNGG	3 cut(s)	94, 103, 527
SetI	ASST	9 cut(s)	19, 48, 87, 98, 183, 193, 224, 320, 489
SfaNI	GCATC	2 cut(s)	354, 632
SmiMI	CAYNNNNRTG	1 cut(s)	359
SphI	GCATGC	1 cut(s)	296
Sse9I	AATT	2 cut(s)	115, 636
SsiI	CCGC	1 cut(s)	5
SspMI	CTAG	2 cut(s)	272, 655
StyD4I	CCNGG	3 cut(s)	92, 101, 525
TaaI	ACNGT	2 cut(s)	169, 227
TaiI	ACGT	1 cut(s)	489
TaqI	TCGA	3 cut(s)	80, 144, 549
TasI	AATT	2 cut(s)	115, 636
TfiI	GAWTC	2 cut(s)	62, 173
Tru1I	TTAA	1 cut(s)	114
Tru9I	TTAA	1 cut(s)	114
TscAI	CASTG	1 cut(s)	211
TseFI	GTSAC	2 cut(s)	17, 283
TseI	GCWGC	1 cut(s)	645
Tsp45I	GTSAC	2 cut(s)	17, 283
TspDTI	ATGAA	1 cut(s)	445
TspGWI	ACGGA	1 cut(s)	573
TspRI	CASTG	1 cut(s)	211
Van91I	CCANNNNNTGG	1 cut(s)	400
XceI	RCATGY	1 cut(s)	296
XmiI	GTMKAC	1 cut(s)	131
XmnI	GAANNNNTTC	1 cut(s)	571
XspI	CTAG	2 cut(s)	272, 655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.