Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0014105

Physical Location & Seq

Genomic Coordinates Reverse (-)

32819888 .. 32823223

Gene Structure 3336 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1268500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGGCTCTCTTCAAAATCCCAAGAAGCTATCTGCTTCTCTCTTCTCCGCACACCTACAACTGCTGCTACTGCTTACGCTTCTCCTCCGCCACCACCACCGCAACCGCAGCCGCAACCAATTGGTACTCGCTCCCGCCAACTTCTCAAAATGAAGACCCAAAACTCTCGGCTATCTCCCACGCCATCAAAAACCACTCTCAGTCTCTCGACTCCTCCTCCTCGCTCAGAAAGCTCCTCCCTTCCCTCACAGCCCGCGATGTTATCAACCTCATCAACCTCAACCCCCACTCTCTCTCCCCTCTCTCTCTCCTCTCCTTCTTCGACTGGCTCTCTTCTCACCCCACATTTCGCCACACCGTCCAATCCTACTGCACCATGGCCCACTTCCTTTGCGCTCACCAAATGTACCCCCAAGCCCTCTCCCTCCTACGACTCGTCGTTTCTCGCAGGGGAAAGGACTCTGCGTCTTCCGTGTTCGCTTCGATTCTCGAAACTAGAGGTACCCATCAGTCCAATTATGTGTTTGATTCTTTGATGAATGCGTATTTGGATTCTGGGTTTGTTTCCGATGCGTTTCAGTGCTTTAGATTGATAAGGAAGAATAATTTTCGAATCCCATTTCATGTTTGTGGCTGTTTGCTTGATAAAATGTTGAAGTTGAACTCGCCTGTGATGGCCTGGGAGTATTTTATGGAGATTTTGGATGCTGGGTTTCCACCACAAGTGTACGCTTTCAATGTTTTGATGCACAAAATGTGTAAAGAGGCTAAAATTGAACAGGCTCAGATGGTGTTTGATGAAATTGGGAAGCGGGGTTTGCGCCCGAGTGTGGTTAGTTTCAATACTCTGATTAATGGGTATTGCAAATCCGGGAATCTGGAGGAGGGTTTTAGGTTGAAGAGAGATATGGAGGAGAGCAGAACATGGCCTGATGTATATACTTACAGTGTTTTGATCAATGGGTTGTGTAAGGAGTGTAGGCTGGATGATGCAAACTACTTGTTTGATGAAATGTGTGAGAGGGGGTTGGTTCCGAATTTTGTCACATTTACGACTTTGATTGACGGGCAGTGTAAGAATGGGAGGATTGATTTGGCAATGGAAATTTATCAGAAAATGTTGGGGAGAGGTATCAAACCAGATGTGATTACGTATAATACACTCATAAATGGCCTTTGCAAGGATGGTGATTTGAGGGAAGCTAGGAAGCTTGTTGAGGAGATGAATATGACTGGGGTGAAGCCTGACACGATCACTTATACTACACTTATTGATGGATGCTGCAAAGAAGGAGATTTACATTCGGCGCTAGAGATAAGGCAGGGAATGATTATACAAGGGATTAAGCTTGACAATGTGGCTTTCACAGCCCTTATTTCGGGATTGTGTAGAGAGGGAAGAACCCTTGACGCTGAAAAAATGTTGAGGGAGATGTTAGATTCTGGCATGAAACCTGATGATGCCACGTATACAATGGTCATTGATGGGTTTTGCAAGAAGGGCAATGTTAAAATGGGTTTTAAGTTGCTCAAGGAGATGCAGAGTGATGACTATGTGCCAAGTGTTGTAACCTATAACGCTCTTATGAATGGGCTATGCAAGCTAGGACAGATGAAAAATGCTAATATGCTCTTACATGCTATGATTAATTTGGGGGTGGCTCCAGATGACATTACATACAACATTCTATTGGAAGGGCATTGCAAGCATGGAAACCCAGAGAACTTTGAAAAGCTACGGAGCGAGAAAGGGCTTGCTCTTGATTATGCTTCATATACTTCGCTAGTTAGCGAATTCAATAAGTCTTCCAAGGATCGTCGAAAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

610

Amino Acids

68.72

Weight (kDa)

8.11

Isoelectric Point (pI)

37.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	155 - 267	4.1e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	232 - 287	5.9e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	240 - 278	1.3e-06	PPR repeat family
TPR_24	PF23276	253 - 373	1.3e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	264 - 320	4.1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	271 - 304	5.3e-12	PPR repeat
PPR_2	PF13041	275 - 324	4.9e-19	PPR repeat family
PPR	PF01535	278 - 306	1.9e-07	PPR repeat
PPR_3	PF13812	298 - 355	4.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	310 - 338	1.7e-10	PPR repeat
PPR	PF01535	313 - 343	5.6e-07	PPR repeat
PPR_long	PF17177	314 - 423	5.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	318 - 359	1.7e-12	PPR repeat family
PPR_3	PF13812	334 - 390	1.2e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	341 - 373	4.1e-11	PPR repeat
PPR_2	PF13041	345 - 394	3e-19	PPR repeat family
PPR	PF01535	348 - 378	1.2e-07	PPR repeat
PPR_long	PF17177	363 - 465	7.7e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	376 - 408	1.3e-16	PPR repeat
PPR_2	PF13041	380 - 429	1.8e-21	PPR repeat family
PPR	PF01535	383 - 409	2.6e-08	PPR repeat
PPR_3	PF13812	403 - 443	9.6e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	412 - 443	1.5e-11	PPR repeat
PPR	PF01535	418 - 447	2.6e-06	PPR repeat
PPR_2	PF13041	418 - 464	3.8e-11	PPR repeat family
PPR_long	PF17177	422 - 576	4e-14	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	447 - 478	6.8e-10	PPR repeat
PPR_2	PF13041	451 - 499	1.8e-15	PPR repeat family
PPR	PF01535	453 - 482	6.5e-07	PPR repeat
PPR_3	PF13812	474 - 530	9.6e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	482 - 514	1.7e-10	PPR repeat
PPR	PF01535	489 - 516	6.1e-07	PPR repeat
PPR_2	PF13041	489 - 534	6.2e-14	PPR repeat family
PPR_3	PF13812	508 - 565	2.5e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	518 - 548	8.8e-08	PPR repeat
PPR_2	PF13041	520 - 569	1.3e-17	PPR repeat family
PPR	PF01535	523 - 553	2.3e-06	PPR repeat
PPR_1	PF12854	552 - 572	8.2e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09680
fragaria_vesca	FvH4_1g30171
malus_domestica	MD17G1268500.v1.1
prunus_persica	Prupe.3G166000_v2.0.a1
pyrus_communis	pycom17g26700
rosa_chinensis	RchiOBHm_Chr3g0491601
rosa_laevigata	RLG00000022834
rosa_roxburghii	Rroxscaffold_6G00392790
rosa_rugosa	Rorug03G0250400 Rorug03G0250500 Rorug03G0250600 Rorug03G0250600 Rorug03G0250600
rosa_samantha	Rh3AG299400 Rh3CG332200 Rh3DG333900
rosa_wichuraiana	Rw3G026450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	500
AccB1I	GGYRCC	1 cut(s)	500
AccI	GTMKAC	1 cut(s)	1469
AccII	CGCG	1 cut(s)	255
AciI	CCGC	8 cut(s)	47, 87, 99, 105, 111, 134, 253, 811
AclWI	GGATC	1 cut(s)	1821
AcsI	RAATTY	3 cut(s)	1036, 1104, 1793
AfaI	GTAC	4 cut(s)	125, 407, 502, 728
AfiI	CCNNNNNNNGG	3 cut(s)	829, 1180, 1378
AgsI	TTSAA	9 cut(s)	13, 655, 661, 736, 776, 841, 898, 1730, 1798
AhdI	GACNNNNNGTC	1 cut(s)	463
AjnI	CCWGG	1 cut(s)	677
AjuI	GAANNNNNNNTTGG	2 cut(s)	530, 562
AluBI	AGCT	7 cut(s)	27, 232, 1202, 1210, 1348, 1603, 1735
AluI	AGCT	7 cut(s)	27, 232, 1202, 1210, 1348, 1603, 1735
Alw26I	GTCTC	1 cut(s)	207
AlwI	GGATC	1 cut(s)	1821
Ama87I	CYCGRG	1 cut(s)	823
AoxI	GGCC	4 cut(s)	378, 675, 926, 1171
ApeKI	GCWGC	3 cut(s)	63, 107, 1281
ApoI	RAATTY	3 cut(s)	1036, 1104, 1793
AseI	ATTAAT	2 cut(s)	852, 1647
Asp718I	GGTACC	1 cut(s)	500
AspLEI	GCGC	3 cut(s)	395, 822, 1309
AspS9I	GGNCC	1 cut(s)	379
AsuC2I	CCSGG	1 cut(s)	871
AsuHPI	GGTGA	4 cut(s)	329, 389, 1199, 1249
AsuII	TTCGAA	1 cut(s)	610
AvaI	CYCGRG	1 cut(s)	823
BanI	GGYRCC	1 cut(s)	500
BarI	GAAGNNNNNNTAC	2 cut(s)	1281, 1313
BbsI	GAAGAC	3 cut(s)	159, 459, 1797
BbvI	GCAGC	3 cut(s)	50, 119, 1268
BccI	CCATC	7 cut(s)	191, 513, 667, 781, 1178, 1268, 1478
BciT130I	CCWGG	1 cut(s)	679
BclI	TGATCA	1 cut(s)	954
BcnI	CCSGG	1 cut(s)	871
BcoDI	GTCTC	1 cut(s)	207
BfaI	CTAG	5 cut(s)	495, 1203, 1310, 1604, 1784
BfoI	RGCGCY	1 cut(s)	1310
BisI	GCNGC	4 cut(s)	64, 108, 111, 1282
BlsI	GCNGC	4 cut(s)	65, 109, 112, 1283
Bme1390I	CCNGG	2 cut(s)	679, 871
BmeRI	GACNNNNNGTC	1 cut(s)	463
BmeT110I	CYCGRG	1 cut(s)	823
BmgT120I	GGNCC	1 cut(s)	379
BmiI	GGNNCC	3 cut(s)	502, 1032, 1662
BmrFI	CCNGG	2 cut(s)	679, 871
BmrI	ACTGGG	1 cut(s)	1242
BmsI	GCATC	7 cut(s)	559, 694, 735, 979, 1268, 1450, 1527
BmuI	ACTGGG	1 cut(s)	1242
BpiI	GAAGAC	3 cut(s)	159, 459, 1797
BpmI	CTGGAG	2 cut(s)	899, 1647
Bpu14I	TTCGAA	1 cut(s)	610
BpuEI	CTTGAG	1 cut(s)	1514
BpuMI	CCSGG	1 cut(s)	871
BsaAI	YACGTR	2 cut(s)	1152, 1467
BsaJI	CCNNGG	3 cut(s)	375, 678, 1809
BsaXI	ACNNNNNCTCC	4 cut(s)	200, 230, 278, 308
Bsc4I	CCNNNNNNNGG	3 cut(s)	829, 1180, 1378
Bse1I	ACTGG	2 cut(s)	329, 1237
Bse3DI	GCAATG	3 cut(s)	1104, 1510, 1699
BseBI	CCWGG	1 cut(s)	679
BseDI	CCNNGG	3 cut(s)	375, 678, 1809
BseGI	GGATG	4 cut(s)	709, 991, 1189, 1283
BseLI	CCNNNNNNNGG	3 cut(s)	829, 1180, 1378
BseMI	GCAATG	3 cut(s)	1104, 1510, 1699
BseMII	CTCAG	3 cut(s)	212, 238, 797
BseNI	ACTGG	2 cut(s)	329, 1237
BseRI	GAGGAG	9 cut(s)	73, 202, 205, 208, 224, 299, 896, 926, 1232
BseXI	GCAGC	3 cut(s)	50, 119, 1268
BseYI	CCCAGC	1 cut(s)	707
BsgI	GTGCAG	1 cut(s)	355
Bsh1236I	CGCG	1 cut(s)	255
BshFI	GGCC	4 cut(s)	380, 677, 928, 1173
BshNI	GGYRCC	1 cut(s)	500
BsiHKCI	CYCGRG	1 cut(s)	823
BsiSI	CCGG	1 cut(s)	870
BslI	CCNNNNNNNGG	3 cut(s)	829, 1180, 1378
BsmAI	GTCTC	1 cut(s)	207
BsmI	GAATGC	1 cut(s)	544
BsnI	GGCC	4 cut(s)	380, 677, 928, 1173
BsoBI	CYCGRG	1 cut(s)	823
Bsp119I	TTCGAA	1 cut(s)	610
Bsp143I	GATC	3 cut(s)	954, 1251, 1813
Bsp19I	CCATGG	1 cut(s)	375
BspACI	CCGC	8 cut(s)	47, 87, 99, 105, 111, 134, 253, 811
BspANI	GGCC	4 cut(s)	380, 677, 928, 1173
BspCNI	CTCAG	3 cut(s)	211, 237, 796
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	3 cut(s)	502, 1032, 1662
BspPI	GGATC	1 cut(s)	1821
BspT104I	TTCGAA	1 cut(s)	610
BspT107I	GGYRCC	1 cut(s)	500
BsrDI	GCAATG	3 cut(s)	1104, 1510, 1699
BsrI	ACTGG	2 cut(s)	329, 1237
BssECI	CCNNGG	3 cut(s)	375, 678, 1809
BssMI	GATC	3 cut(s)	954, 1251, 1813
BssNAI	GTATAC	1 cut(s)	1470
BssT1I	CCWWGG	2 cut(s)	375, 1809
Bst1107I	GTATAC	1 cut(s)	1470
Bst2UI	CCWGG	1 cut(s)	679
Bst4CI	ACNGT	2 cut(s)	358, 947
Bst6I	CTCTTC	4 cut(s)	14, 46, 337, 893
BstBAI	YACGTR	2 cut(s)	1152, 1467
BstBI	TTCGAA	1 cut(s)	610
BstC8I	GCNNGC	4 cut(s)	253, 1601, 1706, 1755
BstDEI	CTNAG	3 cut(s)	198, 224, 783
BstDSI	CCRYGG	1 cut(s)	375
BstENI	CCTNNNNNAGG	1 cut(s)	1178
BstF5I	GGATG	4 cut(s)	709, 991, 1189, 1283
BstFNI	CGCG	1 cut(s)	255
BstH2I	RGCGCY	1 cut(s)	1310
BstHHI	GCGC	3 cut(s)	395, 822, 1309
BstKTI	GATC	3 cut(s)	957, 1254, 1816
BstMAI	GTCTC	1 cut(s)	207
BstMBI	GATC	3 cut(s)	954, 1251, 1813
BstMWI	GCNNNNNNNGC	8 cut(s)	69, 107, 229, 817, 1367, 1500, 1600, 1705
BstNI	CCWGG	1 cut(s)	679
BstNSI	RCATGY	1 cut(s)	1640
BstSCI	CCNGG	2 cut(s)	677, 869
BstSNI	TACGTA	1 cut(s)	1152
BstUI	CGCG	1 cut(s)	255
BstV1I	GCAGC	3 cut(s)	50, 119, 1268
BstV2I	GAAGAC	3 cut(s)	159, 459, 1797
BstZ17I	GTATAC	1 cut(s)	1470
BsuRI	GGCC	4 cut(s)	380, 677, 928, 1173
BtgI	CCRYGG	1 cut(s)	375
BtgZI	GCGATG	1 cut(s)	270
BtsCI	GGATG	4 cut(s)	709, 991, 1189, 1283
BtsI	GCAGTG	1 cut(s)	1076
BtsIMutI	CAGTG	3 cut(s)	584, 952, 1076
Cac8I	GCNNGC	4 cut(s)	253, 1601, 1706, 1755
CfoI	GCGC	3 cut(s)	395, 822, 1309
Cfr13I	GGNCC	1 cut(s)	379
CseI	GACGC	2 cut(s)	453, 1418
Csp6I	GTAC	4 cut(s)	124, 406, 501, 727
CspCI	CAANNNNNGTGG	2 cut(s)	371, 406
CviAII	CATG	6 cut(s)	376, 623, 924, 1447, 1637, 1709
CviQI	GTAC	4 cut(s)	124, 406, 501, 727
DdeI	CTNAG	3 cut(s)	198, 224, 783
DpnI	GATC	3 cut(s)	956, 1253, 1815
DpnII	GATC	3 cut(s)	954, 1251, 1813
DriI	GACNNNNNGTC	1 cut(s)	463
Eam1104I	CTCTTC	4 cut(s)	14, 46, 337, 893
Eam1105I	GACNNNNNGTC	1 cut(s)	463
EarI	CTCTTC	4 cut(s)	14, 46, 337, 893
EciI	GGCGGA	1 cut(s)	76
Eco105I	TACGTA	1 cut(s)	1152
Eco130I	CCWWGG	2 cut(s)	375, 1809
Eco88I	CYCGRG	1 cut(s)	823
EcoNI	CCTNNNNNAGG	1 cut(s)	1178
EcoRI	GAATTC	1 cut(s)	1793
EcoRII	CCWGG	1 cut(s)	677
EcoT14I	CCWWGG	2 cut(s)	375, 1809
ErhI	CCWWGG	2 cut(s)	375, 1809
FaeI	CATG	6 cut(s)	379, 626, 927, 1450, 1640, 1712
FatI	CATG	6 cut(s)	375, 622, 923, 1446, 1636, 1708
FauI	CCCGC	3 cut(s)	141, 260, 804
FbaI	TGATCA	1 cut(s)	954
FblI	GTMKAC	1 cut(s)	1469
Fnu4HI	GCNGC	4 cut(s)	64, 108, 111, 1282
FokI	GGATG	4 cut(s)	716, 998, 1196, 1290
Fsp4HI	GCNGC	4 cut(s)	64, 108, 111, 1282
FspBI	CTAG	5 cut(s)	495, 1203, 1310, 1604, 1784
GlaI	GCGC	3 cut(s)	394, 821, 1308
GluI	GCNGC	4 cut(s)	64, 108, 111, 1282
GsaI	CCCAGC	1 cut(s)	711
GsuI	CTGGAG	2 cut(s)	899, 1647
HaeII	RGCGCY	1 cut(s)	1310
HaeIII	GGCC	4 cut(s)	380, 677, 928, 1173
HapII	CCGG	1 cut(s)	870
HgaI	GACGC	2 cut(s)	453, 1418
HhaI	GCGC	3 cut(s)	395, 822, 1309
Hin1II	CATG	6 cut(s)	379, 626, 927, 1450, 1640, 1712
Hin6I	GCGC	3 cut(s)	393, 820, 1307
HinP1I	GCGC	3 cut(s)	393, 820, 1307
HindIII	AAGCTT	2 cut(s)	1208, 1346
HinfI	GANTC	9 cut(s)	209, 432, 458, 484, 527, 551, 612, 874, 1439
HpaII	CCGG	1 cut(s)	870
HphI	GGTGA	4 cut(s)	329, 389, 1199, 1249
Hpy166II	GTNNAC	2 cut(s)	727, 1470
Hpy188I	TCNGA	6 cut(s)	227, 568, 786, 849, 1035, 1113
Hpy188III	TCNNGA	6 cut(s)	206, 488, 878, 1380, 1664, 1760
Hpy8I	GTNNAC	2 cut(s)	727, 1470
Hpy99I	CGWCG	2 cut(s)	440, 1821
HpyAV	CCTTC	5 cut(s)	249, 325, 1283, 1492, 1688
HpyCH4III	ACNGT	2 cut(s)	358, 947
HpyCH4IV	ACGT	2 cut(s)	1151, 1466
HpyF10VI	GCNNNNNNNGC	8 cut(s)	69, 107, 229, 817, 1367, 1500, 1600, 1705
HpyF3I	CTNAG	3 cut(s)	198, 224, 783
HpySE526I	ACGT	2 cut(s)	1151, 1466
Hsp92II	CATG	6 cut(s)	379, 626, 927, 1450, 1640, 1712
HspAI	GCGC	3 cut(s)	393, 820, 1307
KpnI	GGTACC	1 cut(s)	504
Ksp22I	TGATCA	1 cut(s)	954
Kzo9I	GATC	3 cut(s)	954, 1251, 1813
LmnI	GCTCC	4 cut(s)	135, 237, 1666, 1740
Lsp1109I	GCAGC	3 cut(s)	50, 119, 1268
LweI	GCATC	7 cut(s)	559, 694, 735, 979, 1268, 1450, 1527
MaeI	CTAG	5 cut(s)	495, 1203, 1310, 1604, 1784
MaeII	ACGT	2 cut(s)	1151, 1466
MaeIII	GTNAC	2 cut(s)	1042, 1567
MalI	GATC	3 cut(s)	956, 1253, 1815
MboI	GATC	3 cut(s)	954, 1251, 1813
MboII	GAAGA	9 cut(s)	33, 164, 310, 324, 459, 610, 910, 1410, 1797
MfeI	CAATTG	1 cut(s)	118
MluCI	AATT	9 cut(s)	118, 514, 604, 771, 801, 1036, 1104, 1648, 1793
MlyI	GAGTC	3 cut(s)	203, 426, 452
MseI	TTAA	5 cut(s)	852, 1344, 1509, 1521, 1647
MslI	CAYNNNNRTG	1 cut(s)	627
MspI	CCGG	1 cut(s)	870
MspR9I	CCNGG	2 cut(s)	679, 871
MunI	CAATTG	1 cut(s)	118
Mva1269I	GAATGC	1 cut(s)	544
MvaI	CCWGG	1 cut(s)	679
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	8 cut(s)	69, 107, 229, 817, 1367, 1500, 1600, 1705
NciI	CCSGG	1 cut(s)	871
NcoI	CCATGG	1 cut(s)	375
NdeII	GATC	3 cut(s)	954, 1251, 1813
NlaIII	CATG	6 cut(s)	379, 626, 927, 1450, 1640, 1712
NlaIV	GGNNCC	3 cut(s)	502, 1032, 1662
NmeAIII	GCCGAG	1 cut(s)	146
NmuCI	GTSAC	1 cut(s)	1042
NspI	RCATGY	1 cut(s)	1640
NspV	TTCGAA	1 cut(s)	610
PctI	GAATGC	1 cut(s)	544
PfeI	GAWTC	6 cut(s)	484, 527, 551, 612, 874, 1439
PfoI	TCCNGGA	1 cut(s)	869
PkrI	GCNGC	4 cut(s)	65, 109, 112, 1283
PleI	GAGTC	3 cut(s)	203, 426, 452
PpsI	GAGTC	3 cut(s)	203, 426, 452
Ppu21I	YACGTR	2 cut(s)	1152, 1467
PshBI	ATTAAT	2 cut(s)	852, 1647
Psp6I	CCWGG	1 cut(s)	677
PspFI	CCCAGC	1 cut(s)	707
PspGI	CCWGG	1 cut(s)	677
PspN4I	GGNNCC	3 cut(s)	502, 1032, 1662
PspPI	GGNCC	1 cut(s)	379
RsaI	GTAC	4 cut(s)	125, 407, 502, 728
RsaNI	GTAC	4 cut(s)	124, 406, 501, 727
RseI	CAYNNNNRTG	1 cut(s)	627
SaqAI	TTAA	5 cut(s)	852, 1344, 1509, 1521, 1647
SatI	GCNGC	4 cut(s)	64, 108, 111, 1282
Sau3AI	GATC	3 cut(s)	954, 1251, 1813
Sau96I	GGNCC	1 cut(s)	379
SchI	GAGTC	3 cut(s)	203, 426, 452
ScrFI	CCNGG	2 cut(s)	679, 871
SfaNI	GCATC	7 cut(s)	559, 694, 735, 979, 1268, 1450, 1527
SfuI	TTCGAA	1 cut(s)	610
SmiMI	CAYNNNNRTG	1 cut(s)	627
SmlI	CTYRAG	1 cut(s)	1529
SmoI	CTYRAG	1 cut(s)	1529
SnaBI	TACGTA	1 cut(s)	1152
Sse9I	AATT	9 cut(s)	118, 514, 604, 771, 801, 1036, 1104, 1648, 1793
SsiI	CCGC	8 cut(s)	47, 87, 99, 105, 111, 134, 253, 811
SspMI	CTAG	5 cut(s)	495, 1203, 1310, 1604, 1784
StyD4I	CCNGG	2 cut(s)	677, 869
StyI	CCWWGG	2 cut(s)	375, 1809
TaaI	ACNGT	2 cut(s)	358, 947
TaiI	ACGT	2 cut(s)	1154, 1469
TaqI	TCGA	6 cut(s)	207, 321, 482, 489, 610, 1819
TasI	AATT	9 cut(s)	118, 514, 604, 771, 801, 1036, 1104, 1648, 1793
TauI	GCSGC	1 cut(s)	113
TfiI	GAWTC	6 cut(s)	484, 527, 551, 612, 874, 1439
Tru1I	TTAA	5 cut(s)	852, 1344, 1509, 1521, 1647
Tru9I	TTAA	5 cut(s)	852, 1344, 1509, 1521, 1647
TscAI	CASTG	3 cut(s)	584, 952, 1076
TseFI	GTSAC	1 cut(s)	1042
TseI	GCWGC	3 cut(s)	63, 107, 1281
Tsp45I	GTSAC	1 cut(s)	1042
TspGWI	ACGGA	2 cut(s)	460, 1753
TspRI	CASTG	3 cut(s)	584, 952, 1076
VspI	ATTAAT	2 cut(s)	852, 1647
XagI	CCTNNNNNAGG	1 cut(s)	1178
XapI	RAATTY	3 cut(s)	1036, 1104, 1793
XceI	RCATGY	1 cut(s)	1640
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1471
XmiI	GTMKAC	1 cut(s)	1469
XspI	CTAG	5 cut(s)	495, 1203, 1310, 1604, 1784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.