Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0014105

Physical Location & Seq

Genomic Coordinates Reverse (-)

23613026 .. 23616533

Gene Structure 3508 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0250500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2667 bp

ATGGCTACAACCTGCACATACCCATTGCTTCTCTCTCTCATCTCCACATTCTTCATTCTCACTTCATCATCATCATCACCACCATCTGAGGCAGACATTCTTCTCACCTTCAAAGCCTCCATTAGAGATTCTTCAAACTCTCTCTCAACTTGGTCCAACACCTCTGAGATCCATCATTGTAATTGGACTGGAATTACTTGCACCACCATATCTTCAGTACTCTCTGTAACTTCTCTAAACCTCCAAAGCTTCAACCTTTCTGGTGAAATCTCATCTTCAGTATGTGAACTTCCCAATCTGTCCCTGCTCAACCTTGCTAACAATTTCTTCAGCCAACCCATTCCTCTCCATCTCTCTCAGTGCACTTCTCTGGAGACTTTGAATCTCAGCAACAATCTCATCTGGGGTCCAATCCCAAGTCAGATTTCTCAGTTTGGTTCTTTGAGAGTGCTTGACTTGAGCAAAAACCATGTTGAGGGAAACATCCCACAAAGCTTTGCCTCACTCAACAAGCTCCAAGTTCTCAACTTGGGAAGCAACTTGATTTCAGGTAATGTGCCTTCTATATTTGGAAATTTGAGTGAGCTAGTTGTGCTTGATGTGTCTCAAAATTCATACTTGATGAGTGAGGTTCCCACTGATATTGGGAAGCTTGTTAAGCTTGAGAAGTTATTCTTGCAAAGCTCAAGTTTTCATGGTGAAATTCCTGATTCTTTGGTGGGTATGCAATATTTGACTGTTTTAGACCTTTCCCAGAACAACTTAACTGGTAGGGTTCCTCAGACACTAGGGACTTCTCTTAAGAACTTAGTGTCTTTTGATGTTTCAGTGAATAGGCTTTCTGGGTTATTCCCAAATGGTATATGTAGTGGAAAGGGCCTTATAAACCTCAGCCTTCATACAAATGTGTTCAATGGTTCAGTACCCAGTTCTATTAGTGAATGCTTAAATCTTGAGAGGTTTGAAATTCAGAACAATTTGTTTTCTGGTGATTTCCCAGTTGAGTTATGGTCACTACCCAAAATAAAGCTCTTAAGAGCTGAAAATAATGGATTTTCTGGAGAAATACCTGACTCAGTGTCGAAGGCTGGTCAATTGGAGCAAGTTCAAATAGATAACAATAGTTTCACTAGCAAAATTCCTCAGGGTCTTGGGTTAGTCAAGAGCTTATATAGATTCTCAGCTTCTTTAAATGGTTTATATGGTGAACTACCTCCAAATTTCTGTGACTCCCCTGTTCTGAGTATTGTAAACCTCTCCCACAATTCTCTTTCGGGTCAAATTCCGGAGCTTAGGAAATGCAGGAAGCTAGTGTCTTTGTCTTTGGCAGACAATAAGCTTAGTGGGAATATCGGGTCATCTCTTGGTGAATTACCAGTACTGACTTATCTTGATCTTTCGGACAATATGCTTAATGGTGAAATCCCTCAGGAGCTTCAGAACTTGAAGCTTGCGCTCTTCAATGTCTCCTTTAATCAGCTTTCTGGTAGAGTCCCCTATTCTCTTATTTCAGGTCTCCCAGCTTCGTTTCTCCAAGGAAATCCAGAACTTTGTGGGCCAGGATTACTCCATTCTTGTTCTGATGACCCGCCAAGGCACCGTTCTTCTGATCTTACAACCTTGACATGTGCCCTGATCTCTGTAGCCTTTGCTGTTGGAACTCTGACTATTGCTGGTGCCTTTATTGCTTATCGTCGATATTACAAGCAAAGACCTCAAACTGGCTTATGGCGTTCGGTTTTCTTTTATCCTCTGAGAGTCACCGAGCATGATTTGATAATGGGAATGGATGAGAAAAGTTCAGGAGGAGGTGTTGGAGTATTTGGTAGAGTCCACATTTTAAGTCTACCAAGCGGGGAACTTGTTGCTGTGAAGAAGCTTGTGAATTTCAGGGTCCATTCATCGAAAGCCTTGAAGGCTGAGATCAAGACCTTGGCCAAAATCAGGCATAAAAATATAGTAAAAGTTCTTGGGTATTGCCATTCAGATGATGCAATATTTCTGATATATGAGTTCTTACAGAGAGGGAGCTTGGGGGACTTGATCTGCAGACCTGATTTTGATTTGCAGTGGAGTGTTAGGTTGAGAATTGCCATTGGTGTTGCTCAAGGGCTGGCATACCTCCACAAGGATTATGTTCCTCATTTACTCCATAGAAATGTCAAATCGAAAAATATCCTTTTGGATGCTGATTTTGAACCAAAGCTCACAGATTTTGGTCTGAACAAAATTCTGGGAGATGCTGCCTTTCAGTCAACAATGGCTTCAGAATCTGCTTTCTCCTGTTACAATGCACCAGAAAACAAGTACAGTAAGAAAGCAACTGAACAAATGGATGTGTACAGCTTTGGTGTTGTAATATTGGAGCTAGTGACGGGCCGGCAAGCGGAGCAAGCTTTACCATCTGAGTCTCTCGATATAGTGAAGTGGGTACGACGAAAGGTCAACATTACAAATGGGGCGGTCCAAGTTCTCGATCCCAAGATCACAAGTTCATCCCAGCAAGAGATGCTAGTAGCTCTAGAAATTGCTTTGCATTGCACTTCTGTGATGCCGGAAAAGAGACCATCAATGTCTGAAGTTGTGAAATCACTTCAGTCCCTCGACTCGATGACTCACACTGCTGTTGTAGACTTTTCTGCCTTTGAGGAGCATTCGGTTGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 PPR_long

Protein Analysis

888

Amino Acids

97.04

Weight (kDa)

6.3

Isoelectric Point (pI)

44.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	28 - 68	3.6e-11	Leucine rich repeat N-terminal domain
LRR_8	PF13855	123 - 182	9.5e-08	Leucine rich repeat
LRR_14	PF23598	142 - 270	4.1e-11	Leucine-rich repeat region
LRR_8	PF13855	147 - 204	8.9e-08	Leucine rich repeat
LRR_14	PF23598	197 - 382	1.9e-06	Leucine-rich repeat region
PK_Tyr_Ser-Thr	PF07714	604 - 865	4.7e-37	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	604 - 863	1.9e-35	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014105)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09680
fragaria_vesca	FvH4_1g30171
malus_domestica	MD17G1268500.v1.1
prunus_persica	Prupe.3G166000_v2.0.a1
pyrus_communis	pycom17g26700
rosa_chinensis	RchiOBHm_Chr3g0491601
rosa_laevigata	RLG00000022834
rosa_roxburghii	Rroxscaffold_6G00392790
rosa_rugosa	Rorug03G0250400 Rorug03G0250500 Rorug03G0250600 Rorug03G0250600 Rorug03G0250600
rosa_samantha	Rh3AG299400 Rh3CG332200 Rh3DG333900
rosa_wichuraiana	Rw3G026450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	884
Acc36I	ACCTGC	1 cut(s)	20
AccB1I	GGYRCC	2 cut(s)	1596, 1676
AccI	GTMKAC	2 cut(s)	1846, 2631
AccIII	TCCGGA	1 cut(s)	1285
AciI	CCGC	4 cut(s)	1589, 1854, 2387, 2462
AclWI	GGATC	2 cut(s)	163, 2471
AcoI	YGGCCR	1 cut(s)	1935
AcsI	RAATTY	9 cut(s)	574, 610, 702, 966, 1137, 1219, 1281, 1885, 2229
AcuI	CTGAAG	7 cut(s)	198, 261, 313, 1421, 2250, 2579, 2598
AfaI	GTAC	6 cut(s)	219, 924, 1380, 2309, 2342, 2433
AfiI	CCNNNNNNNGG	5 cut(s)	475, 1721, 1944, 2128, 2386
AflII	CTTAAG	2 cut(s)	800, 1033
AflIII	ACRYGT	1 cut(s)	1625
AhdI	GACNNNNNGTC	2 cut(s)	1078, 2441
AjnI	CCWGG	1 cut(s)	1558
AjuI	GAANNNNNNNTTGG	4 cut(s)	409, 441, 1585, 1617
AleI	CACNNNNGTG	1 cut(s)	2546
Alw21I	GWGCWC	1 cut(s)	365
Alw26I	GTCTC	6 cut(s)	368, 609, 1471, 1520, 2415, 2557
Alw44I	GTGCAC	1 cut(s)	361
AlwI	GGATC	2 cut(s)	163, 2471
AlwNI	CAGNNNCTG	1 cut(s)	2273
Aor13HI	TCCGGA	1 cut(s)	1285
AoxI	GGCC	4 cut(s)	877, 1556, 1935, 2377
ApaLI	GTGCAC	1 cut(s)	361
ApeKI	GCWGC	1 cut(s)	2243
ApoI	RAATTY	9 cut(s)	574, 610, 702, 966, 1137, 1219, 1281, 1885, 2229
Asp700I	GAANNNNTTC	1 cut(s)	671
AspLEI	GCGC	1 cut(s)	1456
AspS9I	GGNCC	7 cut(s)	153, 407, 877, 1556, 1894, 2377, 2464
AsuHPI	GGTGA	9 cut(s)	69, 97, 275, 710, 1001, 1217, 1379, 1430, 1753
AvaII	GGWCC	4 cut(s)	153, 407, 1894, 2464
AxyI	CCTNAGG	2 cut(s)	1143, 1428
BaeGI	GKGCMC	2 cut(s)	365, 1633
BalI	TGGCCA	1 cut(s)	1937
BanI	GGYRCC	2 cut(s)	1596, 1676
BarI	GAAGNNNNNNTAC	4 cut(s)	273, 305, 544, 576
Bbv12I	GWGCWC	1 cut(s)	365
BbvCI	CCTCAGC	1 cut(s)	890
BbvI	GCAGC	1 cut(s)	2230
BccI	CCATC	5 cut(s)	91, 180, 357, 2410, 2575
BciT130I	CCWGG	1 cut(s)	1560
BcoDI	GTCTC	6 cut(s)	368, 609, 1471, 1520, 2415, 2557
BfaI	CTAG	7 cut(s)	587, 788, 1131, 1310, 2369, 2513, 2522
BfmI	CTRYAG	2 cut(s)	1641, 2047
BfrI	CTTAAG	2 cut(s)	800, 1033
BfuAI	ACCTGC	1 cut(s)	20
BglI	GCCNNNNNGGC	1 cut(s)	1916
BisI	GCNGC	1 cut(s)	2244
BlsI	GCNGC	1 cut(s)	2245
BmcAI	AGTACT	2 cut(s)	219, 1380
Bme1390I	CCNGG	1 cut(s)	1560
Bme18I	GGWCC	4 cut(s)	153, 407, 1894, 2464
BmeRI	GACNNNNNGTC	2 cut(s)	1078, 2441
BmgT120I	GGNCC	7 cut(s)	153, 407, 877, 1556, 1894, 2377, 2464
BmiI	GGNNCC	6 cut(s)	408, 633, 777, 1598, 1678, 1895
BmrFI	CCNGG	1 cut(s)	1560
BmrI	ACTGGG	2 cut(s)	921, 992
BmsI	GCATC	5 cut(s)	1981, 2176, 2230, 2499, 2541
BmuI	ACTGGG	2 cut(s)	921, 992
BpmI	CTGGAG	2 cut(s)	392, 1080
Bpu10I	CCTNAGC	2 cut(s)	890, 1292
BpuEI	CTTGAG	5 cut(s)	478, 670, 683, 974, 2091
BsaI	GGTCTC	2 cut(s)	1520, 2557
BsaJI	CCNNGG	3 cut(s)	1534, 1592, 1932
BsaWI	WCCGGW	1 cut(s)	1285
BsaXI	ACNNNNNCTCC	2 cut(s)	1797, 1827
Bsc4I	CCNNNNNNNGG	5 cut(s)	475, 1721, 1944, 2128, 2386
Bse118I	RCCGGY	1 cut(s)	2379
Bse1I	ACTGG	6 cut(s)	193, 772, 927, 998, 1376, 1726
Bse21I	CCTNAGG	2 cut(s)	1143, 1428
Bse3DI	GCAATG	2 cut(s)	23, 2536
BseAI	TCCGGA	1 cut(s)	1285
BseBI	CCWGG	1 cut(s)	1560
BseDI	CCNNGG	3 cut(s)	1534, 1592, 1932
BseGI	GGATG	5 cut(s)	483, 1795, 2191, 2341, 2495
BseLI	CCNNNNNNNGG	5 cut(s)	475, 1721, 1944, 2128, 2386
BseMI	GCAATG	2 cut(s)	23, 2536
BseNI	ACTGG	6 cut(s)	193, 772, 927, 998, 1376, 1726
BseRI	GAGGAG	2 cut(s)	1821, 2663
BseSI	GKGCMC	2 cut(s)	365, 1633
BseXI	GCAGC	1 cut(s)	2230
BseYI	CCCAGC	2 cut(s)	1519, 2499
BshFI	GGCC	4 cut(s)	879, 1558, 1937, 2379
BshNI	GGYRCC	2 cut(s)	1596, 1676
BsiHKAI	GWGCWC	1 cut(s)	365
BsiSI	CCGG	3 cut(s)	1286, 2380, 2555
BslFI	GGGAC	5 cut(s)	286, 805, 1478, 2051, 2584
BslI	CCNNNNNNNGG	5 cut(s)	475, 1721, 1944, 2128, 2386
BsmAI	GTCTC	6 cut(s)	368, 609, 1471, 1520, 2415, 2557
BsmFI	GGGAC	5 cut(s)	286, 805, 1478, 2051, 2584
BsmI	GAATGC	2 cut(s)	947, 2653
BsnI	GGCC	4 cut(s)	879, 1558, 1937, 2379
Bso31I	GGTCTC	2 cut(s)	1520, 2557
Bsp1286I	GDGCHC	2 cut(s)	365, 1633
Bsp13I	TCCGGA	1 cut(s)	1285
Bsp1407I	TGTACA	1 cut(s)	2340
Bsp143I	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
BspACI	CCGC	4 cut(s)	1589, 1854, 2387, 2462
BspANI	GGCC	4 cut(s)	879, 1558, 1937, 2379
BspEI	TCCGGA	1 cut(s)	1285
BspLI	GGNNCC	6 cut(s)	408, 633, 777, 1598, 1678, 1895
BspMAI	CTGCAG	1 cut(s)	2051
BspMI	ACCTGC	1 cut(s)	20
BspPI	GGATC	2 cut(s)	163, 2471
BspQI	GCTCTTC	1 cut(s)	1463
BspT107I	GGYRCC	2 cut(s)	1596, 1676
BspTI	CTTAAG	2 cut(s)	800, 1033
BspTNI	GGTCTC	2 cut(s)	1520, 2557
BsrDI	GCAATG	2 cut(s)	23, 2536
BsrFI	RCCGGY	1 cut(s)	2379
BsrGI	TGTACA	1 cut(s)	2340
BsrI	ACTGG	6 cut(s)	193, 772, 927, 998, 1376, 1726
BssAI	RCCGGY	1 cut(s)	2379
BssECI	CCNNGG	3 cut(s)	1534, 1592, 1932
BssMI	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
BssT1I	CCWWGG	3 cut(s)	1534, 1592, 1932
Bst2UI	CCWGG	1 cut(s)	1560
Bst4CI	ACNGT	3 cut(s)	739, 1601, 2312
Bst6I	CTCTTC	1 cut(s)	1463
BstAFI	CTTAAG	2 cut(s)	800, 1033
BstAPI	GCANNNNNTGC	1 cut(s)	2509
BstAUI	TGTACA	1 cut(s)	2340
BstC8I	GCNNGC	5 cut(s)	1452, 2115, 2381, 2385, 2394
BstENI	CCTNNNNNAGG	1 cut(s)	2126
BstF5I	GGATG	5 cut(s)	483, 1795, 2191, 2341, 2495
BstHHI	GCGC	1 cut(s)	1456
BstKTI	GATC	8 cut(s)	171, 1396, 1612, 1638, 1926, 2046, 2479, 2487
BstMAI	GTCTC	6 cut(s)	368, 609, 1471, 1520, 2415, 2557
BstMBI	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
BstMWI	GCNNNNNNNGC	7 cut(s)	592, 658, 1685, 1916, 2389, 2393, 2509
BstNI	CCWGG	1 cut(s)	1560
BstNSI	RCATGY	1 cut(s)	1629
BstSCI	CCNGG	1 cut(s)	1558
BstSFI	CTRYAG	2 cut(s)	1641, 2047
BstSLI	GKGCMC	2 cut(s)	365, 1633
BstV1I	GCAGC	1 cut(s)	2230
BstX2I	RGATCY	1 cut(s)	168
BstYI	RGATCY	1 cut(s)	168
Bsu36I	CCTNAGG	2 cut(s)	1143, 1428
BsuRI	GGCC	4 cut(s)	879, 1558, 1937, 2379
BtsCI	GGATG	5 cut(s)	483, 1795, 2191, 2341, 2495
BtsI	GCAGTG	2 cut(s)	2075, 2619
BtsIMutI	CAGTG	6 cut(s)	365, 636, 834, 1083, 2075, 2619
BveI	ACCTGC	1 cut(s)	20
Cac8I	GCNNGC	5 cut(s)	1452, 2115, 2381, 2385, 2394
CaiI	CAGNNNCTG	1 cut(s)	2273
CfoI	GCGC	1 cut(s)	1456
Cfr10I	RCCGGY	1 cut(s)	2379
Cfr13I	GGNCC	7 cut(s)	153, 407, 877, 1556, 1894, 2377, 2464
Csp6I	GTAC	6 cut(s)	218, 923, 1379, 2308, 2341, 2432
CspCI	CAANNNNNGTGG	2 cut(s)	625, 660
CviAII	CATG	4 cut(s)	470, 695, 1626, 1769
CviQI	GTAC	6 cut(s)	218, 923, 1379, 2308, 2341, 2432
DpnI	GATC	8 cut(s)	170, 1395, 1611, 1637, 1925, 2045, 2478, 2486
DpnII	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
DraI	TTTAAA	1 cut(s)	1191
DriI	GACNNNNNGTC	2 cut(s)	1078, 2441
EaeI	YGGCCR	1 cut(s)	1935
Eam1104I	CTCTTC	1 cut(s)	1463
Eam1105I	GACNNNNNGTC	2 cut(s)	1078, 2441
EarI	CTCTTC	1 cut(s)	1463
Eco130I	CCWWGG	3 cut(s)	1534, 1592, 1932
Eco31I	GGTCTC	2 cut(s)	1520, 2557
Eco47I	GGWCC	4 cut(s)	153, 407, 1894, 2464
Eco57I	CTGAAG	7 cut(s)	198, 261, 313, 1421, 2250, 2579, 2598
Eco81I	CCTNAGG	2 cut(s)	1143, 1428
EcoNI	CCTNNNNNAGG	1 cut(s)	2126
EcoO109I	RGGNCCY	1 cut(s)	877
EcoRII	CCWGG	1 cut(s)	1558
EcoT14I	CCWWGG	3 cut(s)	1534, 1592, 1932
ErhI	CCWWGG	3 cut(s)	1534, 1592, 1932
FaeI	CATG	4 cut(s)	473, 698, 1629, 1772
FalI	AAGNNNNNCTT	2 cut(s)	659, 691
FaqI	GGGAC	5 cut(s)	286, 805, 1478, 2051, 2584
FatI	CATG	4 cut(s)	469, 694, 1625, 1768
FauI	CCCGC	2 cut(s)	1596, 1847
FblI	GTMKAC	2 cut(s)	1846, 2631
Fnu4HI	GCNGC	1 cut(s)	2244
FokI	GGATG	5 cut(s)	470, 1802, 2198, 2348, 2482
Fsp4HI	GCNGC	1 cut(s)	2244
FspBI	CTAG	7 cut(s)	587, 788, 1131, 1310, 2369, 2513, 2522
GlaI	GCGC	1 cut(s)	1455
GluI	GCNGC	1 cut(s)	2244
GsaI	CCCAGC	2 cut(s)	1523, 2503
GsuI	CTGGAG	2 cut(s)	392, 1080
HaeIII	GGCC	4 cut(s)	879, 1558, 1937, 2379
HapII	CCGG	3 cut(s)	1286, 2380, 2555
HhaI	GCGC	1 cut(s)	1456
Hin1II	CATG	4 cut(s)	473, 698, 1629, 1772
Hin6I	GCGC	1 cut(s)	1454
HinP1I	GCGC	1 cut(s)	1454
HincII	GTYRAC	2 cut(s)	2256, 2446
HindII	GTYRAC	2 cut(s)	2256, 2446
HindIII	AAGCTT	8 cut(s)	247, 493, 650, 659, 1337, 1448, 1877, 2394
HpaII	CCGG	3 cut(s)	1286, 2380, 2555
HphI	GGTGA	9 cut(s)	69, 97, 275, 710, 1001, 1217, 1379, 1430, 1753
Hpy99I	CGWCG	2 cut(s)	1698, 2439
HpyAV	CCTTC	5 cut(s)	118, 570, 905, 1078, 1909
HpyCH4III	ACNGT	3 cut(s)	739, 1601, 2312
HpyF10VI	GCNNNNNNNGC	7 cut(s)	592, 658, 1685, 1916, 2389, 2393, 2509
Hsp92II	CATG	4 cut(s)	473, 698, 1629, 1772
HspAI	GCGC	1 cut(s)	1454
Kpn2I	TCCGGA	1 cut(s)	1285
KroI	GCCGGC	1 cut(s)	2379
KroNI	GCCGGC	1 cut(s)	2381
Kzo9I	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
LguI	GCTCTTC	1 cut(s)	1463
LmnI	GCTCC	8 cut(s)	519, 1099, 1288, 1432, 2028, 2365, 2389, 2650
Lsp1109I	GCAGC	1 cut(s)	2230
LweI	GCATC	5 cut(s)	1981, 2176, 2230, 2499, 2541
MaeI	CTAG	7 cut(s)	587, 788, 1131, 1310, 2369, 2513, 2522
MaeIII	GTNAC	6 cut(s)	226, 1011, 1226, 1759, 2285, 2371
MalI	GATC	8 cut(s)	170, 1395, 1611, 1637, 1925, 2045, 2478, 2486
MboI	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
MboII	GAAGA	9 cut(s)	43, 92, 123, 204, 267, 319, 1450, 1596, 1885
MfeI	CAATTG	1 cut(s)	1094
MflI	RGATCY	1 cut(s)	168
MhlI	GDGCHC	2 cut(s)	365, 1633
MlsI	TGGCCA	1 cut(s)	1937
MluNI	TGGCCA	1 cut(s)	1937
MlyI	GAGTC	8 cut(s)	1067, 1223, 1500, 1767, 1839, 2417, 2600, 2608
MmeI	TCCRAC	3 cut(s)	180, 1636, 1795
Mox20I	TGGCCA	1 cut(s)	1937
MroI	TCCGGA	1 cut(s)	1285
MroNI	GCCGGC	1 cut(s)	2379
MroXI	GAANNNNTTC	1 cut(s)	671
MscI	TGGCCA	1 cut(s)	1937
MseI	TTAA	9 cut(s)	657, 764, 801, 947, 1034, 1190, 1413, 1473, 1841
MslI	CAYNNNNRTG	4 cut(s)	903, 1986, 2157, 2546
Msp20I	TGGCCA	1 cut(s)	1937
MspCI	CTTAAG	2 cut(s)	800, 1033
MspI	CCGG	3 cut(s)	1286, 2380, 2555
MspR9I	CCNGG	1 cut(s)	1560
MunI	CAATTG	1 cut(s)	1094
Mva1269I	GAATGC	2 cut(s)	947, 2653
MvaI	CCWGG	1 cut(s)	1560
MwoI	GCNNNNNNNGC	7 cut(s)	592, 658, 1685, 1916, 2389, 2393, 2509
NaeI	GCCGGC	1 cut(s)	2381
NdeII	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
NgoMIV	GCCGGC	1 cut(s)	2379
NlaIII	CATG	4 cut(s)	473, 698, 1629, 1772
NlaIV	GGNNCC	6 cut(s)	408, 633, 777, 1598, 1678, 1895
NmuCI	GTSAC	4 cut(s)	1011, 1226, 1759, 2371
NspI	RCATGY	1 cut(s)	1629
OliI	CACNNNNGTG	1 cut(s)	2546
PciI	ACATGT	1 cut(s)	1625
PciSI	GCTCTTC	1 cut(s)	1463
PctI	GAATGC	2 cut(s)	947, 2653
PdiI	GCCGGC	1 cut(s)	2381
PdmI	GAANNNNTTC	1 cut(s)	671
PfeI	GAWTC	5 cut(s)	128, 382, 710, 1176, 2270
PkrI	GCNGC	1 cut(s)	2245
PleI	GAGTC	8 cut(s)	1067, 1223, 1499, 1766, 1838, 2416, 2600, 2608
PpsI	GAGTC	8 cut(s)	1067, 1223, 1499, 1766, 1838, 2416, 2600, 2608
PscI	ACATGT	1 cut(s)	1625
PsiI	TTATAA	1 cut(s)	884
Psp6I	CCWGG	1 cut(s)	1558
PspFI	CCCAGC	2 cut(s)	1519, 2499
PspGI	CCWGG	1 cut(s)	1558
PspN4I	GGNNCC	6 cut(s)	408, 633, 777, 1598, 1678, 1895
PspPI	GGNCC	7 cut(s)	153, 407, 877, 1556, 1894, 2377, 2464
PstI	CTGCAG	1 cut(s)	2051
PstNI	CAGNNNCTG	1 cut(s)	2273
PsuI	RGATCY	1 cut(s)	168
RsaI	GTAC	6 cut(s)	219, 924, 1380, 2309, 2342, 2433
RsaNI	GTAC	6 cut(s)	218, 923, 1379, 2308, 2341, 2432
RseI	CAYNNNNRTG	4 cut(s)	903, 1986, 2157, 2546
SapI	GCTCTTC	1 cut(s)	1463
SaqAI	TTAA	9 cut(s)	657, 764, 801, 947, 1034, 1190, 1413, 1473, 1841
SatI	GCNGC	1 cut(s)	2244
Sau3AI	GATC	8 cut(s)	168, 1393, 1609, 1635, 1923, 2043, 2476, 2484
Sau96I	GGNCC	7 cut(s)	153, 407, 877, 1556, 1894, 2377, 2464
ScaI	AGTACT	2 cut(s)	219, 1380
SchI	GAGTC	8 cut(s)	1067, 1223, 1500, 1767, 1839, 2417, 2600, 2608
ScrFI	CCNGG	1 cut(s)	1560
SduI	GDGCHC	2 cut(s)	365, 1633
SfaNI	GCATC	5 cut(s)	1981, 2176, 2230, 2499, 2541
SfcI	CTRYAG	2 cut(s)	1641, 2047
SinI	GGWCC	4 cut(s)	153, 407, 1894, 2464
SmiMI	CAYNNNNRTG	4 cut(s)	903, 1986, 2157, 2546
SmlI	CTYRAG	7 cut(s)	457, 662, 685, 800, 953, 1033, 2106
SmoI	CTYRAG	7 cut(s)	457, 662, 685, 800, 953, 1033, 2106
SsiI	CCGC	4 cut(s)	1589, 1854, 2387, 2462
SspI	AATATT	3 cut(s)	731, 1998, 2361
SspMI	CTAG	7 cut(s)	587, 788, 1131, 1310, 2369, 2513, 2522
StyD4I	CCNGG	1 cut(s)	1558
StyI	CCWWGG	3 cut(s)	1534, 1592, 1932
TaaI	ACNGT	3 cut(s)	739, 1601, 2312
TaqI	TCGA	8 cut(s)	1082, 1696, 1904, 2168, 2415, 2475, 2604, 2609
TatI	WGTACW	4 cut(s)	217, 1378, 2307, 2340
TfiI	GAWTC	5 cut(s)	128, 382, 710, 1176, 2270
Tru1I	TTAA	9 cut(s)	657, 764, 801, 947, 1034, 1190, 1413, 1473, 1841
Tru9I	TTAA	9 cut(s)	657, 764, 801, 947, 1034, 1190, 1413, 1473, 1841
TscAI	CASTG	6 cut(s)	365, 643, 834, 1083, 2075, 2626
TseFI	GTSAC	4 cut(s)	1011, 1226, 1759, 2371
TseI	GCWGC	1 cut(s)	2243
Tsp45I	GTSAC	4 cut(s)	1011, 1226, 1759, 2371
TspDTI	ATGAA	7 cut(s)	43, 54, 603, 683, 887, 1890, 2484
TspRI	CASTG	6 cut(s)	365, 643, 834, 1083, 2075, 2626
Vha464I	CTTAAG	2 cut(s)	800, 1033
VneI	GTGCAC	1 cut(s)	361
VpaK11BI	GGWCC	4 cut(s)	153, 407, 1894, 2464
XagI	CCTNNNNNAGG	1 cut(s)	2126
XapI	RAATTY	9 cut(s)	574, 610, 702, 966, 1137, 1219, 1281, 1885, 2229
XbaI	TCTAGA	1 cut(s)	2521
XceI	RCATGY	1 cut(s)	1629
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1005
XmiI	GTMKAC	2 cut(s)	1846, 2631
XmnI	GAANNNNTTC	1 cut(s)	671
XspI	CTAG	7 cut(s)	587, 788, 1131, 1310, 2369, 2513, 2522
ZrmI	AGTACT	2 cut(s)	219, 1380

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.