Rw3G026450

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0014105
Physical Location & Seq
Forward (+)
36316091 .. 36319532
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G026450.1

Sequence Viewer

Length: 1782 bp
ATGGCAAGGAGCAATCAGTTTCTGCTTCCTCCCCACAATTACAAATTATGCTTCTCCACCACCCCTTGGTACAAGCCTCCTCCTCCTCGCCGCCGTGAACATGACAAAGAGGAACCGTTACTCTCCCTCCTCTCCGACGCCATCACCACCAGCAACACAAACCCTCTCCCTCTCAGGAAGCTCCTCCCTTCCCTCACCGCCCGTGACGTCATCAACCTCATCAACCTCAACCCCGACTCCCTCCCTCCCCACTCTCTCCTCTCCTTCTTCCACTGGCTCTCTTCCCACCCCACCTTTCGCCACACCCTCCAATCCTACCTCACCATGGCCCACTTCCTCTCCTCCCACCAAATGTTCCCCCAAGCCCTCTCCCTCCTACGACTCGTCGTTTCCCGCAAGGCCAAGGGCTCCGCCTCCTCCGTCTTCGCTTCCCTTCTCCAAACCAGAGGTACCCATCTCCACTCCGATTACGTCTTTCATGCTTTGATCAATTCCTACACGGATTCTGGGTTCCTGGGGGATGCCGTGCAGTGCTTTAGGTTGCTTAGGAAGCACGGTTTTCGGATACCCTTTCAGGCTTGTGGTCATTTGCTTGATAAAATGTTCATGTTCAACACCCCTGTTGTCGCTTGGGGCTTTCTTTTGGAGATTTTGGATTCTGGGTTTCCACCCAACGTGTATAATTTCAATGTTTTGATGCATAAAATGTGCAAGCAAGGTCATATCAGAGAGGCCCAGGCGGTTTTCGATGAAATTGGGAACAGGGGTTTGAACCCAACTGTAGTTAGTTTTAATACCTTGATTAATGGCTATTGTAAATCTGGCAATCTAGAGCAGGGTTTTAAGTTGAAGGCGGATATGGAGGGGAGAACAATGTGTCCTGATTTGTTTACTTACAGTGTTTTAATAAATGGGTTGTGTAAGGAAGGCAGGTTGGATGATGCATATGGGTTGTTTGATGAAATGTGTGTCAAGGGTTTGGTTCCGAATGACGTCATATTCACGACTTTGATTGATGGGCAGTGTAAGAATGGGAATCTTGATCTGGCGCTGGAAATGTATCAGAAAATGTTAGGAAGAGGTATCAAACCAGATATAGTAACATACAATACACTCATAAATGGCCTTTGCAAGGTTGGAGATATGAAAGAAGCTAGGAAGCTTGTCGAAGAGATGAATACGAGAGGTTTCAAGCCTGATAAGATTACTTATACTACACTTATTGATGGATGCTGCAAAGAAGGAGATTTAGAATCGGCCATAGAGATAAGGAAGGAGATGATTAAAGAAGAGATTGCACTCGACAATGTAGCTTTCACAGCCCTTATTTCAGGCTTTTGCAGAGAGGGAAGAATCATCGAGGCAGAAAAAATGTTGAGGGAGATGTTAAAGGCCGGCATGAAGCCTGATGATGCCACTTATACAATGCTCATTGATGGTTTTTGCAAAACGGGTGATGTTAATATTGGTTTTAAGTTGCTCAAGGAAATGCAGGGTGATGGTCATGTACCAAGTGTTGTGACCTATAATGTGCTTATGAATGGATTATGCAAGCAAGGACAGATGAAAAATGCAAATATGCTGTTGAATGCTATGATTAACGTGGGGGTGGTGCCCGATGATATTACATACAACACTCTATTAGAAGGACATTGCAAGCATGGAAACCCAGATGACTTTGAGAAGCTATGGAGTGAGAAAGGACTTGTTCTTGATTATGCTTCGTATAATTCTCTAGTCAGTGAATTACATAAATCTTTAAAAAAGCGTCCGAAGAGATGA

Protein Analysis

593

Amino Acids

66.78

Weight (kDa)

8.21

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 218 - 271 2.6e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 221 - 251 1.5e-06 PPR repeat
PPR_2 PF13041 224 - 264 7.3e-08 PPR repeat family
TPR_24 PF23276 234 - 357 8.2e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 255 - 288 4.9e-11 PPR repeat
PPR_2 PF13041 259 - 308 1.1e-17 PPR repeat family
PPR PF01535 262 - 289 1e-05 PPR repeat
PPR_1 PF12854 292 - 322 1.3e-14 PPR repeat
PPR PF01535 297 - 327 5.9e-09 PPR repeat
PPR_2 PF13041 302 - 332 1.5e-07 PPR repeat family
PPR_long PF17177 311 - 408 2.1e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 318 - 374 3.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 325 - 357 8.1e-10 PPR repeat
PPR_2 PF13041 329 - 378 1.7e-18 PPR repeat family
PPR PF01535 332 - 362 3.9e-07 PPR repeat
PPR_1 PF12854 360 - 392 8e-16 PPR repeat
PPR_2 PF13041 364 - 413 1.1e-22 PPR repeat family
PPR PF01535 367 - 397 1.7e-09 PPR repeat
PPR_3 PF13812 387 - 444 4.4e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 395 - 427 8e-13 PPR repeat
PPR PF01535 402 - 431 8.9e-09 PPR repeat
PPR_2 PF13041 402 - 448 4.6e-13 PPR repeat family
PPR_long PF17177 404 - 560 3.1e-14 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 433 - 462 5.1e-09 PPR repeat
PPR_2 PF13041 435 - 483 1.2e-16 PPR repeat family
PPR PF01535 437 - 466 5.1e-08 PPR repeat
PPR_3 PF13812 458 - 514 1.5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 466 - 498 1.5e-10 PPR repeat
PPR PF01535 473 - 501 2.3e-06 PPR repeat
PPR_2 PF13041 473 - 518 5.4e-15 PPR repeat family
PPR_3 PF13812 492 - 549 3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 500 - 532 3.3e-09 PPR repeat
PPR_2 PF13041 504 - 553 1.2e-17 PPR repeat family
PPR PF01535 507 - 537 5.9e-06 PPR repeat
PPR_1 PF12854 536 - 564 2e-07 PPR repeat
PPR_2 PF13041 539 - 584 8.9e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014105)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09680
fragaria_vesca FvH4_1g30171
malus_domestica MD17G1268500.v1.1
prunus_persica Prupe.3G166000_v2.0.a1
pyrus_communis pycom17g26700
rosa_chinensis RchiOBHm_Chr3g0491601
rosa_laevigata RLG00000022834
rosa_roxburghii Rroxscaffold_6G00392790
rosa_rugosa Rorug03G0250400 Rorug03G0250500 Rorug03G0250600 Rorug03G0250600 Rorug03G0250600
rosa_samantha Rh3AG299400 Rh3CG332200 Rh3DG333900
rosa_wichuraiana Rw3G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 210, 996
Acc36I ACCTGC 1 cut(s) 921
Acc65I GGTACC 1 cut(s) 449
AccB1I GGYRCC 2 cut(s) 449, 1612
AccB7I CCANNNNNTGG 1 cut(s) 66
AciI CCGC 6 cut(s) 91, 198, 394, 411, 740, 854
AcoI YGGCCR 1 cut(s) 1257
AcyI GRCGYC 3 cut(s) 138, 207, 993
AfaI GTAC 3 cut(s) 71, 451, 1509
AfiI CCNNNNNNNGG 3 cut(s) 66, 325, 1132
AflIII ACRYGT 1 cut(s) 675
AgsI TTSAA 6 cut(s) 613, 688, 772, 850, 1192, 1588
AjnI CCWGG 2 cut(s) 513, 735
AloI GAACNNNNNNTCC 4 cut(s) 494, 526, 862, 894
AluBI AGCT 5 cut(s) 181, 1154, 1162, 1313, 1687
AluI AGCT 5 cut(s) 181, 1154, 1162, 1313, 1687
AlwNI CAGNNNCTG 1 cut(s) 22
AoxI GGCC 6 cut(s) 327, 399, 732, 1123, 1257, 1392
ApeKI GCWGC 1 cut(s) 1233
AseI ATTAAT 1 cut(s) 804
Asp718I GGTACC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 1051
AspS9I GGNCC 2 cut(s) 328, 733
AsuHPI GGTGA 5 cut(s) 136, 187, 313, 1466, 1508
BaeGI GKGCMC 1 cut(s) 1617
BanI GGYRCC 2 cut(s) 449, 1612
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 415
BbvI GCAGC 1 cut(s) 1220
BccI CCATC 6 cut(s) 149, 462, 1010, 1220, 1430, 1493
BceAI ACGGC 2 cut(s) 78, 509
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BciT130I CCWGG 2 cut(s) 515, 737
BciVI GTATCC 1 cut(s) 558
BclI TGATCA 1 cut(s) 486
BfaI CTAG 3 cut(s) 830, 1155, 1736
BfmI CTRYAG 1 cut(s) 780
BfoI RGCGCY 1 cut(s) 1052
BfuAI ACCTGC 1 cut(s) 921
BfuI GTATCC 1 cut(s) 558
BisI GCNGC 2 cut(s) 91, 1234
BlsI GCNGC 2 cut(s) 92, 1235
Bme1390I CCNGG 2 cut(s) 515, 737
BmgT120I GGNCC 2 cut(s) 328, 733
BmiI GGNNCC 6 cut(s) 114, 409, 451, 512, 984, 1614
BmrFI CCNGG 2 cut(s) 515, 737
BmsI GCATC 5 cut(s) 511, 687, 931, 1220, 1402
BpiI GAAGAC 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 1466
BsaHI GRCGYC 3 cut(s) 138, 207, 993
BsaJI CCNNGG 5 cut(s) 65, 324, 402, 514, 735
BsaXI ACNNNNNCTCC 6 cut(s) 111, 141, 221, 251, 323, 353
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 325, 1132
Bse118I RCCGGY 1 cut(s) 1394
Bse1I ACTGG 1 cut(s) 278
Bse3DI GCAATG 1 cut(s) 1651
BseBI CCWGG 2 cut(s) 515, 737
BseDI CCNNGG 5 cut(s) 65, 324, 402, 514, 735
BseGI GGATG 3 cut(s) 526, 943, 1235
BseLI CCNNNNNNNGG 3 cut(s) 66, 325, 1132
BseMI GCAATG 1 cut(s) 1651
BseMII CTCAG 1 cut(s) 187
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 8 cut(s) 69, 72, 75, 119, 173, 248, 331, 406
BseSI GKGCMC 1 cut(s) 1617
BseXI GCAGC 1 cut(s) 1220
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 6 cut(s) 329, 401, 734, 1125, 1259, 1394
BshNI GGYRCC 2 cut(s) 449, 1612
BsiSI CCGG 1 cut(s) 1395
BslI CCNNNNNNNGG 3 cut(s) 66, 325, 1132
BsmI GAATGC 1 cut(s) 1594
BsnI GGCC 6 cut(s) 329, 401, 734, 1125, 1259, 1394
Bsp1286I GDGCHC 2 cut(s) 410, 1617
Bsp143I GATC 2 cut(s) 486, 1042
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 6 cut(s) 91, 198, 394, 411, 740, 854
BspANI GGCC 6 cut(s) 329, 401, 734, 1125, 1259, 1394
BspCNI CTCAG 1 cut(s) 186
BspLI GGNNCC 6 cut(s) 114, 409, 451, 512, 984, 1614
BspMI ACCTGC 1 cut(s) 921
BspT107I GGYRCC 2 cut(s) 449, 1612
BsrDI GCAATG 1 cut(s) 1651
BsrFI RCCGGY 1 cut(s) 1394
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 1394
BssECI CCNNGG 5 cut(s) 65, 324, 402, 514, 735
BssMI GATC 2 cut(s) 486, 1042
BssNI GRCGYC 3 cut(s) 138, 207, 993
BssT1I CCWWGG 3 cut(s) 65, 324, 402
Bst2UI CCWGG 2 cut(s) 515, 737
Bst4CI ACNGT 4 cut(s) 117, 557, 781, 899
Bst6I CTCTTC 5 cut(s) 286, 1072, 1164, 1284, 1769
BstACI GRCGYC 3 cut(s) 138, 207, 993
BstC8I GCNNGC 4 cut(s) 713, 1396, 1553, 1658
BstDEI CTNAG 2 cut(s) 173, 545
BstDSI CCRYGG 1 cut(s) 324
BstENI CCTNNNNNAGG 1 cut(s) 1130
BstF5I GGATG 3 cut(s) 526, 943, 1235
BstH2I RGCGCY 1 cut(s) 1052
BstHHI GCGC 1 cut(s) 1051
BstKTI GATC 2 cut(s) 489, 1045
BstMBI GATC 2 cut(s) 486, 1042
BstMWI GCNNNNNNNGC 2 cut(s) 550, 1319
BstNI CCWGG 2 cut(s) 515, 737
BstSCI CCNGG 2 cut(s) 513, 735
BstSFI CTRYAG 1 cut(s) 780
BstSLI GKGCMC 1 cut(s) 1617
BstV1I GCAGC 1 cut(s) 1220
BstV2I GAAGAC 1 cut(s) 415
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 6 cut(s) 329, 401, 734, 1125, 1259, 1394
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 3 cut(s) 526, 943, 1235
BtsI GCAGTG 2 cut(s) 536, 1028
BtsIMutI CAGTG 5 cut(s) 271, 536, 904, 1028, 1747
BveI ACCTGC 1 cut(s) 921
Cac8I GCNNGC 4 cut(s) 713, 1396, 1553, 1658
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 1 cut(s) 1051
Cfr10I RCCGGY 1 cut(s) 1394
Cfr13I GGNCC 2 cut(s) 328, 733
CseI GACGC 2 cut(s) 146, 1757
Csp6I GTAC 3 cut(s) 70, 450, 1508
CviAII CATG 7 cut(s) 101, 325, 479, 607, 1399, 1505, 1661
CviQI GTAC 3 cut(s) 70, 450, 1508
DdeI CTNAG 2 cut(s) 173, 545
DpnI GATC 2 cut(s) 488, 1044
DpnII GATC 2 cut(s) 486, 1042
DraI TTTAAA 1 cut(s) 1761
EaeI YGGCCR 1 cut(s) 1257
Eam1104I CTCTTC 5 cut(s) 286, 1072, 1164, 1284, 1769
EarI CTCTTC 5 cut(s) 286, 1072, 1164, 1284, 1769
EciI GGCGGA 2 cut(s) 400, 869
Eco130I CCWWGG 3 cut(s) 65, 324, 402
Eco24I GRGCYC 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 1130
EcoRII CCWGG 2 cut(s) 513, 735
EcoT14I CCWWGG 3 cut(s) 65, 324, 402
EcoT22I ATGCAT 2 cut(s) 702, 946
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 3 cut(s) 65, 324, 402
FaeI CATG 7 cut(s) 104, 328, 482, 610, 1402, 1508, 1664
FatI CATG 7 cut(s) 100, 324, 478, 606, 1398, 1504, 1660
FauI CCCGC 1 cut(s) 401
FauNDI CATATG 1 cut(s) 946
FbaI TGATCA 1 cut(s) 486
Fnu4HI GCNGC 2 cut(s) 91, 1234
FokI GGATG 3 cut(s) 533, 950, 1242
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 2 cut(s) 91, 1234
FspBI CTAG 3 cut(s) 830, 1155, 1736
GlaI GCGC 1 cut(s) 1050
GluI GCNGC 2 cut(s) 91, 1234
HaeII RGCGCY 1 cut(s) 1052
HaeIII GGCC 6 cut(s) 329, 401, 734, 1125, 1259, 1394
HapII CCGG 1 cut(s) 1395
HgaI GACGC 2 cut(s) 146, 1757
HhaI GCGC 1 cut(s) 1051
Hin1I GRCGYC 3 cut(s) 138, 207, 993
Hin1II CATG 7 cut(s) 104, 328, 482, 610, 1402, 1508, 1664
Hin6I GCGC 1 cut(s) 1049
HinP1I GCGC 1 cut(s) 1049
HindIII AAGCTT 1 cut(s) 1160
HinfI GANTC 7 cut(s) 236, 381, 503, 656, 1036, 1253, 1353
HpaII CCGG 1 cut(s) 1395
HphI GGTGA 5 cut(s) 136, 187, 313, 1466, 1508
Hpy166II GTNNAC 2 cut(s) 98, 891
Hpy188I TCNGA 7 cut(s) 136, 466, 564, 728, 987, 1065, 1773
Hpy188III TCNNGA 6 cut(s) 175, 830, 881, 1003, 1040, 1712
Hpy8I GTNNAC 2 cut(s) 98, 891
Hpy99I CGWCG 2 cut(s) 140, 389
HpyAV CCTTC 8 cut(s) 198, 274, 443, 844, 920, 1235, 1267, 1640
HpyCH4III ACNGT 4 cut(s) 117, 557, 781, 899
HpyCH4IV ACGT 5 cut(s) 207, 471, 675, 993, 1602
HpyF10VI GCNNNNNNNGC 2 cut(s) 550, 1319
HpyF3I CTNAG 2 cut(s) 173, 545
HpySE526I ACGT 5 cut(s) 207, 471, 675, 993, 1602
Hsp92I GRCGYC 3 cut(s) 138, 207, 993
Hsp92II CATG 7 cut(s) 104, 328, 482, 610, 1402, 1508, 1664
HspAI GCGC 1 cut(s) 1049
KpnI GGTACC 1 cut(s) 453
KroI GCCGGC 1 cut(s) 1394
KroNI GCCGGC 1 cut(s) 1396
Ksp22I TGATCA 1 cut(s) 486
Kzo9I GATC 2 cut(s) 486, 1042
LmnI GCTCC 3 cut(s) 9, 186, 413
Lsp1109I GCAGC 1 cut(s) 1220
LweI GCATC 5 cut(s) 511, 687, 931, 1220, 1402
MaeI CTAG 3 cut(s) 830, 1155, 1736
MaeII ACGT 5 cut(s) 207, 471, 675, 993, 1602
MaeIII GTNAC 4 cut(s) 117, 203, 1099, 1519
MalI GATC 2 cut(s) 488, 1044
MboI GATC 2 cut(s) 486, 1042
MboII GAAGA 7 cut(s) 259, 273, 415, 1089, 1181, 1301, 1362
MhlI GDGCHC 2 cut(s) 410, 1617
MluCI AATT 7 cut(s) 37, 44, 490, 682, 753, 1729, 1745
MlyI GAGTC 2 cut(s) 230, 375
MmeI TCCRAC 3 cut(s) 159, 915, 1117
Mph1103I ATGCAT 2 cut(s) 702, 946
MroNI GCCGGC 1 cut(s) 1394
MspI CCGG 1 cut(s) 1395
MspR9I CCNGG 2 cut(s) 515, 737
Mva1269I GAATGC 1 cut(s) 1594
MvaI CCWGG 2 cut(s) 515, 737
MwoI GCNNNNNNNGC 2 cut(s) 550, 1319
NaeI GCCGGC 1 cut(s) 1396
NcoI CCATGG 1 cut(s) 324
NdeI CATATG 1 cut(s) 946
NdeII GATC 2 cut(s) 486, 1042
NgoMIV GCCGGC 1 cut(s) 1394
NlaIII CATG 7 cut(s) 104, 328, 482, 610, 1402, 1508, 1664
NlaIV GGNNCC 6 cut(s) 114, 409, 451, 512, 984, 1614
NmuCI GTSAC 2 cut(s) 203, 1519
NsiI ATGCAT 2 cut(s) 702, 946
PctI GAATGC 1 cut(s) 1594
PdiI GCCGGC 1 cut(s) 1396
PfeI GAWTC 5 cut(s) 503, 656, 1036, 1253, 1353
PflMI CCANNNNNTGG 1 cut(s) 66
PkrI GCNGC 2 cut(s) 92, 1235
PleI GAGTC 2 cut(s) 230, 375
PpsI GAGTC 2 cut(s) 230, 375
PshBI ATTAAT 1 cut(s) 804
Psp6I CCWGG 2 cut(s) 513, 735
PspGI CCWGG 2 cut(s) 513, 735
PspN4I GGNNCC 6 cut(s) 114, 409, 451, 512, 984, 1614
PspPI GGNCC 2 cut(s) 328, 733
PstNI CAGNNNCTG 1 cut(s) 22
RsaI GTAC 3 cut(s) 71, 451, 1509
RsaNI GTAC 3 cut(s) 70, 450, 1508
SatI GCNGC 2 cut(s) 91, 1234
Sau3AI GATC 2 cut(s) 486, 1042
Sau96I GGNCC 2 cut(s) 328, 733
SchI GAGTC 2 cut(s) 230, 375
ScrFI CCNGG 2 cut(s) 515, 737
SduI GDGCHC 2 cut(s) 410, 1617
SfaNI GCATC 5 cut(s) 511, 687, 931, 1220, 1402
SfcI CTRYAG 1 cut(s) 780
SmlI CTYRAG 1 cut(s) 1481
SmoI CTYRAG 1 cut(s) 1481
Sse9I AATT 7 cut(s) 37, 44, 490, 682, 753, 1729, 1745
SsiI CCGC 6 cut(s) 91, 198, 394, 411, 740, 854
SspI AATATT 1 cut(s) 1465
SspMI CTAG 3 cut(s) 830, 1155, 1736
StyD4I CCNGG 2 cut(s) 513, 735
StyI CCWWGG 3 cut(s) 65, 324, 402
TaaI ACNGT 4 cut(s) 117, 557, 781, 899
TaiI ACGT 5 cut(s) 210, 474, 678, 996, 1605
TaqI TCGA 4 cut(s) 747, 1167, 1302, 1359
TasI AATT 7 cut(s) 37, 44, 490, 682, 753, 1729, 1745
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 5 cut(s) 503, 656, 1036, 1253, 1353
TscAI CASTG 5 cut(s) 278, 536, 904, 1028, 1747
TseFI GTSAC 2 cut(s) 203, 1519
TseI GCWGC 1 cut(s) 1233
Tsp45I GTSAC 2 cut(s) 203, 1519
TspDTI ATGAA 9 cut(s) 467, 595, 765, 975, 1160, 1190, 1415, 1553, 1580
TspGWI ACGGA 2 cut(s) 409, 515
TspRI CASTG 5 cut(s) 278, 536, 904, 1028, 1747
Van91I CCANNNNNTGG 1 cut(s) 66
VspI ATTAAT 1 cut(s) 804
XagI CCTNNNNNAGG 1 cut(s) 1130
XbaI TCTAGA 1 cut(s) 829
XspI CTAG 3 cut(s) 830, 1155, 1736
ZraI GACGTC 2 cut(s) 208, 994
Zsp2I ATGCAT 2 cut(s) 702, 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.