Prupe.1G250700_v2.0.a1

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015883
Physical Location & Seq
Forward (+)
26253437 .. 26257067
3631 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G250700.1

Sequence Viewer

Length: 1689 bp
ATGGCCATAGTTGCATCACTACCAGGCTTCAATCTCTTCTCTTCGCTCCCTTCAAACCCACCCCCAAACGACCCAAATTCCAGCACCACCTCTGCTTCTCCTTCCCCACCAATCCCAATTCCTAAATACCCTCCTCCCCTCAAAAAGTCCCAGAATGCCCCTCCCAACCCGGCCCTCAAAACCGTCCACCGCCGCACCAAGTACTACAAGCCCGTACGAGACGGCGTCATCTCCTCCGACGGCGACCGAGCCGTCGTCATCGGCGAAAACGGCGTGTCATATCTCCTCCCCGGCGCACCCTTCGAGTTCCAGTACAGCTACTCCGAGACCCCAAAGGTCAAGCCTTTGGCCATTCGTGAACCGGCCTTCTTGCCCTTCGCGCCGCCCACAATGCCGAGACCGTGGACCGGCAAAGCTCCATTGAAGAGCGCCAAGGAGAAGAAGCTGAAGCGCAAAGTTCCGCTCTTGGACTCGTTCGGTCCAATGGCGCCTGGAACGAGTGGAGTGAAGGAGGTGGTAATGGCGAGGCCCGTTGAGCTGGGCAAGTACCCAAAGCAAGAAAAGACAAGGGAGGAGATTTTAGGAGAGCCCTTGAAGAAATGGGAGATCAAAATGCTAATCCAGCCCCAATTATCAGATAATCGCCAAGTCAATCTCGGAAGAGATGGGTTGACTCATAACATGTTGGATTTGATACATTCGCACTGGAAGCGGCGACCAGTCTGTAAAGTAAAATGCAAAGGTGTTCCAACTGTGGATATGGATAATGTTTGCCGTCATATTGAGGAAAGAACTAAGGGGAAGATCATTCATCGAGTTGGTGGTGTAGTTTATCTTTTCCGCGGAAGAAACTATAGCTATAAAGATCGTCCTCAGTACCCATTGATGCTGTGGAAACCAGCTGCTCCAGTCTACCCCAAACTTATCCAAGAGGCTCCAGAAGGGTTGACAAAGGATCAAGCTGAAGAGTTTCGGAAGAAAGGGAAAAGACTTTTGCCAATATGTAAATTAGCCAAAAATGGAGTATACATCACCTTGGTGAGAGATGTTAGGCATGCTTTTGAAGGAAGTCCTTTAGTAAAAATTGACTGCAGAGGGATGCATGCAAGTGATTACAAAAAATTGGGTGCTAAGCTTAAGGAATTGGTTCCTTGTGTTCTACTTTCTTTTGATGATGAACATATATTGATGTGGAGGGGTCCAAATTGGATATCAATGCACCAAGCAGCACCTTCACCTTCAGATACTGCAGATTTTGATATTCTATCATCAACAGGGAGGGGCAAGGATTATGATAAACCTTGTAAGCCTGATACCAAAACAGTGATGACAAGCCCCAAAATGATGTCACTTTGGAAACGCTCAATTGATTCAAACAAAGCCCTGTTGCTGGATGAACTTGACCTTGATCCTGATGCGCTTTTGAAGAAAGTCGAGGAATTTGAGGGTGTTTCACAGGCGACAGAGCACTCGTATCCAGCATTGATCGTGTCAAGTGAAGATGGCACGAGCAGCTCAGTTCAAGTGTTTGAAGAGGTTCCTCAAAGTGACCCTTACAGTGAATATGACGATTACAGCGACAATTATGATTATGAGAGTGATGATGATGATGCATTTTATGATAGTGATTCTATCCCCTTGGGGTCATTACCTGTTGATGTAATAGTAGAGGAACTGGATCATGATTAA

Protein Analysis

563

Amino Acids

62.98

Weight (kDa)

7.96

Isoelectric Point (pI)

52.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23400
fragaria_vesca FvH4_4g32880
malus_domestica MD13G1094700.v1.1
prunus_persica Prupe.1G250700_v2.0.a1
pyrus_communis pycom13g08250
rosa_chinensis RchiOBHm_Chr4g0441911
rosa_laevigata RLG00000006057
rosa_multiflora Rmu_co8453927.1_g000001
rosa_roxburghii Rroxscaffold_5G00382720
rosa_rugosa Rorug04G0336900
rosa_samantha Rh4AG388600 Rh4BG402300 Rh4CG417400 Rh4DG395600
rosa_wichuraiana Rw4G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AccB1I GGYRCC 1 cut(s) 487
AccBSI CCGCTC 1 cut(s) 463
AccI GTMKAC 2 cut(s) 912, 1026
AccII CGCG 2 cut(s) 380, 843
AciI CCGC 7 cut(s) 190, 193, 383, 461, 712, 841, 843
AclWI GGATC 3 cut(s) 963, 1403, 1686
AcoI YGGCCR 2 cut(s) 3, 348
AcsI RAATTY 2 cut(s) 76, 1439
AcuI CTGAAG 3 cut(s) 467, 984, 1224
AcyI GRCGYC 2 cut(s) 225, 488
AfaI GTAC 5 cut(s) 203, 216, 314, 548, 878
AfiI CCNNNNNNNGG 2 cut(s) 407, 1390
AflII CTTAAG 1 cut(s) 1136
AflIII ACRYGT 1 cut(s) 681
AgsI TTSAA 9 cut(s) 31, 54, 424, 595, 1064, 1374, 1426, 1523, 1532
AjnI CCWGG 2 cut(s) 22, 490
AleI CACNNNNGTG 1 cut(s) 1037
AluBI AGCT 9 cut(s) 318, 416, 445, 538, 858, 902, 962, 1135, 1515
AluI AGCT 9 cut(s) 318, 416, 445, 538, 858, 902, 962, 1135, 1515
Alw21I GWGCWC 1 cut(s) 1470
Alw26I GTCTC 3 cut(s) 213, 320, 391
AlwI GGATC 3 cut(s) 963, 1403, 1686
AlwNI CAGNNNCTG 1 cut(s) 1247
AoxI GGCC 5 cut(s) 3, 171, 348, 363, 527
ApeKI GCWGC 3 cut(s) 902, 1226, 1512
ApoI RAATTY 2 cut(s) 76, 1439
Asp700I GAANNNNTTC 3 cut(s) 969, 1146, 1536
AspLEI GCGC 6 cut(s) 296, 382, 431, 453, 490, 1420
AspS9I GGNCC 5 cut(s) 172, 405, 479, 528, 1199
AsuC2I CCSGG 2 cut(s) 170, 291
AsuHPI GGTGA 3 cut(s) 1024, 1051, 1227
AvaII GGWCC 3 cut(s) 405, 479, 1199
BalI TGGCCA 2 cut(s) 5, 350
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 1 cut(s) 591
BauI CACGAG 1 cut(s) 1507
Bbv12I GWGCWC 1 cut(s) 1470
BbvI GCAGC 3 cut(s) 889, 1238, 1524
BccI CCATC 2 cut(s) 659, 1496
BceAI ACGGC 5 cut(s) 236, 238, 256, 286, 759
BciT130I CCWGG 2 cut(s) 24, 492
BciVI GTATCC 1 cut(s) 1485
BcnI CCSGG 2 cut(s) 170, 291
BcoDI GTCTC 3 cut(s) 213, 320, 391
BfmI CTRYAG 3 cut(s) 853, 1090, 1248
BfoI RGCGCY 2 cut(s) 432, 491
BfrI CTTAAG 1 cut(s) 1136
BfuI GTATCC 1 cut(s) 1485
BisI GCNGC 6 cut(s) 193, 383, 713, 903, 1227, 1513
BlpI GCTNAGC 1 cut(s) 1131
BlsI GCNGC 6 cut(s) 194, 384, 714, 904, 1228, 1514
BmcAI AGTACT 1 cut(s) 203
Bme1390I CCNGG 4 cut(s) 24, 170, 291, 492
Bme18I GGWCC 3 cut(s) 405, 479, 1199
BmgT120I GGNCC 5 cut(s) 172, 405, 479, 528, 1199
BmiI GGNNCC 5 cut(s) 489, 936, 1149, 1200, 1539
BmrFI CCNGG 4 cut(s) 24, 170, 291, 492
BmsI GCATC 5 cut(s) 23, 876, 1089, 1405, 1600
BpmI CTGGAG 2 cut(s) 891, 921
Bpu1102I GCTNAGC 1 cut(s) 1131
BpuMI CCSGG 2 cut(s) 170, 291
BsaHI GRCGYC 2 cut(s) 225, 488
BsaI GGTCTC 2 cut(s) 320, 391
BsaJI CCNNGG 6 cut(s) 289, 401, 432, 841, 1035, 1638
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 1390
Bse118I RCCGGY 2 cut(s) 361, 407
Bse1I ACTGG 5 cut(s) 310, 710, 719, 908, 1680
BseBI CCWGG 2 cut(s) 24, 492
BseDI CCNNGG 6 cut(s) 289, 401, 432, 841, 1035, 1638
BseGI GGATG 2 cut(s) 1104, 1399
BseLI CCNNNNNNNGG 2 cut(s) 407, 1390
BseMII CTCAG 2 cut(s) 887, 1530
BseNI ACTGG 5 cut(s) 310, 710, 719, 908, 1680
BseRI GAGGAG 4 cut(s) 123, 223, 275, 587
BseXI GCAGC 3 cut(s) 889, 1238, 1524
BseYI CCCAGC 1 cut(s) 538
Bsh1236I CGCG 2 cut(s) 380, 843
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 5 cut(s) 5, 173, 350, 365, 529
BshNI GGYRCC 1 cut(s) 487
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 1470
BsiSI CCGG 4 cut(s) 170, 291, 362, 408
BsiWI CGTACG 1 cut(s) 214
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 2 cut(s) 407, 1390
BsmAI GTCTC 3 cut(s) 213, 320, 391
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 5 cut(s) 5, 173, 350, 365, 529
Bso31I GGTCTC 2 cut(s) 320, 391
Bsp1286I GDGCHC 2 cut(s) 591, 1470
Bsp143I GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
Bsp1720I GCTNAGC 1 cut(s) 1131
BspACI CCGC 7 cut(s) 190, 193, 383, 461, 712, 841, 843
BspANI GGCC 5 cut(s) 5, 173, 350, 365, 529
BspCNI CTCAG 2 cut(s) 886, 1529
BspFNI CGCG 2 cut(s) 380, 843
BspHI TCATGA 1 cut(s) 1681
BspLI GGNNCC 5 cut(s) 489, 936, 1149, 1200, 1539
BspMAI CTGCAG 2 cut(s) 1094, 1252
BspPI GGATC 3 cut(s) 963, 1403, 1686
BspQI GCTCTTC 1 cut(s) 419
BspT107I GGYRCC 1 cut(s) 487
BspTI CTTAAG 1 cut(s) 1136
BspTNI GGTCTC 2 cut(s) 320, 391
BsrBI CCGCTC 1 cut(s) 463
BsrFI RCCGGY 2 cut(s) 361, 407
BsrI ACTGG 5 cut(s) 310, 710, 719, 908, 1680
BssAI RCCGGY 2 cut(s) 361, 407
BssECI CCNNGG 6 cut(s) 289, 401, 432, 841, 1035, 1638
BssMI GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
BssNAI GTATAC 1 cut(s) 1027
BssNI GRCGYC 2 cut(s) 225, 488
BssSI CACGAG 1 cut(s) 1507
BssT1I CCWWGG 3 cut(s) 432, 1035, 1638
Bst1107I GTATAC 1 cut(s) 1027
Bst2BI CACGAG 1 cut(s) 1507
Bst2UI CCWGG 2 cut(s) 24, 492
Bst4CI ACNGT 5 cut(s) 184, 402, 754, 1324, 1559
Bst6I CTCTTC 6 cut(s) 41, 46, 419, 655, 960, 1527
BstACI GRCGYC 2 cut(s) 225, 488
BstAFI CTTAAG 1 cut(s) 1136
BstC8I GCNNGC 2 cut(s) 1056, 1104
BstDEI CTNAG 4 cut(s) 795, 873, 1131, 1516
BstDSI CCRYGG 2 cut(s) 401, 841
BstF5I GGATG 2 cut(s) 1104, 1399
BstFNI CGCG 2 cut(s) 380, 843
BstH2I RGCGCY 2 cut(s) 432, 491
BstHHI GCGC 6 cut(s) 296, 382, 431, 453, 490, 1420
BstKTI GATC 7 cut(s) 609, 807, 868, 958, 1411, 1488, 1681
BstMAI GTCTC 3 cut(s) 213, 320, 391
BstMBI GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 8 cut(s) 11, 270, 379, 391, 535, 622, 709, 1512
BstNI CCWGG 2 cut(s) 24, 492
BstNSI RCATGY 3 cut(s) 685, 1058, 1106
BstSCI CCNGG 4 cut(s) 22, 168, 289, 490
BstSFI CTRYAG 3 cut(s) 853, 1090, 1248
BstUI CGCG 2 cut(s) 380, 843
BstV1I GCAGC 3 cut(s) 889, 1238, 1524
BstZ17I GTATAC 1 cut(s) 1027
BsuI GTATCC 1 cut(s) 1485
BsuRI GGCC 5 cut(s) 5, 173, 350, 365, 529
BtgI CCRYGG 2 cut(s) 401, 841
BtsCI GGATG 2 cut(s) 1104, 1399
BtsIMutI CAGTG 3 cut(s) 703, 1329, 1564
Cac8I GCNNGC 2 cut(s) 1056, 1104
CaiI CAGNNNCTG 1 cut(s) 1247
CciI TCATGA 1 cut(s) 1681
CfoI GCGC 6 cut(s) 296, 382, 431, 453, 490, 1420
Cfr10I RCCGGY 2 cut(s) 361, 407
Cfr13I GGNCC 5 cut(s) 172, 405, 479, 528, 1199
Cfr42I CCGCGG 1 cut(s) 844
CseI GACGC 1 cut(s) 214
Csp6I GTAC 5 cut(s) 202, 215, 313, 547, 877
CviAII CATG 4 cut(s) 682, 1055, 1103, 1682
CviQI GTAC 5 cut(s) 202, 215, 313, 547, 877
DdeI CTNAG 4 cut(s) 795, 873, 1131, 1516
DinI GGCGCC 1 cut(s) 489
DpnI GATC 7 cut(s) 608, 806, 867, 957, 1410, 1487, 1680
DpnII GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 2 cut(s) 3, 348
Eam1104I CTCTTC 6 cut(s) 41, 46, 419, 655, 960, 1527
EarI CTCTTC 6 cut(s) 41, 46, 419, 655, 960, 1527
Eco130I CCWWGG 3 cut(s) 432, 1035, 1638
Eco24I GRGCYC 1 cut(s) 591
Eco31I GGTCTC 2 cut(s) 320, 391
Eco32I GATATC 1 cut(s) 1212
Eco47I GGWCC 3 cut(s) 405, 479, 1199
Eco57I CTGAAG 3 cut(s) 467, 984, 1224
EcoRII CCWGG 2 cut(s) 22, 490
EcoRV GATATC 1 cut(s) 1212
EcoT14I CCWWGG 3 cut(s) 432, 1035, 1638
EcoT22I ATGCAT 2 cut(s) 1104, 1615
EcoT38I GRGCYC 1 cut(s) 591
EgeI GGCGCC 1 cut(s) 489
EheI GGCGCC 1 cut(s) 489
ErhI CCWWGG 3 cut(s) 432, 1035, 1638
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 4 cut(s) 685, 1058, 1106, 1685
FalI AAGNNNNNCTT 2 cut(s) 1537, 1569
FaqI GGGAC 1 cut(s) 133
FatI CATG 4 cut(s) 681, 1054, 1102, 1681
FblI GTMKAC 2 cut(s) 912, 1026
Fnu4HI GCNGC 6 cut(s) 193, 383, 713, 903, 1227, 1513
FokI GGATG 2 cut(s) 1111, 1406
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 6 cut(s) 193, 383, 713, 903, 1227, 1513
GlaI GCGC 6 cut(s) 295, 381, 430, 452, 489, 1419
GluI GCNGC 6 cut(s) 193, 383, 713, 903, 1227, 1513
GsaI CCCAGC 1 cut(s) 542
GsuI CTGGAG 2 cut(s) 891, 921
HaeII RGCGCY 2 cut(s) 432, 491
HaeIII GGCC 5 cut(s) 5, 173, 350, 365, 529
HapII CCGG 4 cut(s) 170, 291, 362, 408
HgaI GACGC 1 cut(s) 214
HhaI GCGC 6 cut(s) 296, 382, 431, 453, 490, 1420
Hin1I GRCGYC 2 cut(s) 225, 488
Hin1II CATG 4 cut(s) 685, 1058, 1106, 1685
Hin6I GCGC 6 cut(s) 294, 380, 429, 451, 488, 1418
HinP1I GCGC 6 cut(s) 294, 380, 429, 451, 488, 1418
HincII GTYRAC 2 cut(s) 672, 948
HindII GTYRAC 2 cut(s) 672, 948
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 4 cut(s) 470, 673, 1370, 1628
HpaII CCGG 4 cut(s) 170, 291, 362, 408
HphI GGTGA 3 cut(s) 1024, 1051, 1227
Hpy166II GTNNAC 7 cut(s) 187, 359, 405, 672, 913, 948, 1027
Hpy188I TCNGA 6 cut(s) 238, 325, 637, 659, 975, 1243
Hpy188III TCNNGA 4 cut(s) 356, 938, 1412, 1682
Hpy8I GTNNAC 7 cut(s) 187, 359, 405, 672, 913, 948, 1027
Hpy99I CGWCG 2 cut(s) 242, 257
HpyCH4III ACNGT 5 cut(s) 184, 402, 754, 1324, 1559
HpyCH4V TGCA 8 cut(s) 14, 738, 1092, 1102, 1106, 1219, 1250, 1613
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 270, 379, 391, 535, 622, 709, 1512
HpyF3I CTNAG 4 cut(s) 795, 873, 1131, 1516
Hsp92I GRCGYC 2 cut(s) 225, 488
Hsp92II CATG 4 cut(s) 685, 1058, 1106, 1685
HspAI GCGC 6 cut(s) 294, 380, 429, 451, 488, 1418
KasI GGCGCC 1 cut(s) 487
KspI CCGCGG 1 cut(s) 844
Kzo9I GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
LguI GCTCTTC 1 cut(s) 419
LmnI GCTCC 4 cut(s) 51, 421, 910, 940
Lsp1109I GCAGC 3 cut(s) 889, 1238, 1524
LweI GCATC 5 cut(s) 23, 876, 1089, 1405, 1600
MaeIII GTNAC 2 cut(s) 1347, 1547
MalI GATC 7 cut(s) 608, 806, 867, 957, 1410, 1487, 1680
MbiI CCGCTC 1 cut(s) 463
MboI GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
MfeI CAATTG 1 cut(s) 1365
MhlI GDGCHC 2 cut(s) 591, 1470
MlsI TGGCCA 2 cut(s) 5, 350
MluNI TGGCCA 2 cut(s) 5, 350
Mly113I GGCGCC 1 cut(s) 488
MlyI GAGTC 2 cut(s) 464, 667
MmeI TCCRAC 3 cut(s) 261, 666, 773
Mox20I TGGCCA 2 cut(s) 5, 350
Mph1103I ATGCAT 2 cut(s) 1104, 1615
MroXI GAANNNNTTC 3 cut(s) 969, 1146, 1536
MscI TGGCCA 2 cut(s) 5, 350
MseI TTAA 2 cut(s) 1137, 1687
MslI CAYNNNNRTG 2 cut(s) 1037, 1107
Msp20I TGGCCA 2 cut(s) 5, 350
MspA1I CMGCKG 2 cut(s) 843, 902
MspCI CTTAAG 1 cut(s) 1136
MspI CCGG 4 cut(s) 170, 291, 362, 408
MspR9I CCNGG 4 cut(s) 24, 170, 291, 492
MunI CAATTG 1 cut(s) 1365
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 2 cut(s) 24, 492
MvnI CGCG 2 cut(s) 380, 843
MwoI GCNNNNNNNGC 8 cut(s) 11, 270, 379, 391, 535, 622, 709, 1512
NarI GGCGCC 1 cut(s) 488
NciI CCSGG 2 cut(s) 170, 291
NdeII GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
NlaIII CATG 4 cut(s) 685, 1058, 1106, 1685
NlaIV GGNNCC 5 cut(s) 489, 936, 1149, 1200, 1539
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 2 cut(s) 1347, 1547
NsiI ATGCAT 2 cut(s) 1104, 1615
NspI RCATGY 3 cut(s) 685, 1058, 1106
OliI CACNNNNGTG 1 cut(s) 1037
PaeI GCATGC 2 cut(s) 1058, 1106
PagI TCATGA 1 cut(s) 1681
PciI ACATGT 1 cut(s) 681
PciSI GCTCTTC 1 cut(s) 419
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 1575
PctI GAATGC 1 cut(s) 160
PdmI GAANNNNTTC 3 cut(s) 969, 1146, 1536
PfeI GAWTC 2 cut(s) 1370, 1628
Pfl23II CGTACG 1 cut(s) 214
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 6 cut(s) 194, 384, 714, 904, 1228, 1514
PleI GAGTC 2 cut(s) 464, 667
PluTI GGCGCC 1 cut(s) 491
PpsI GAGTC 2 cut(s) 464, 667
PscI ACATGT 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 22, 490
PspFI CCCAGC 1 cut(s) 538
PspGI CCWGG 2 cut(s) 22, 490
PspLI CGTACG 1 cut(s) 214
PspN4I GGNNCC 5 cut(s) 489, 936, 1149, 1200, 1539
PspPI GGNCC 5 cut(s) 172, 405, 479, 528, 1199
PstI CTGCAG 2 cut(s) 1094, 1252
PstNI CAGNNNCTG 1 cut(s) 1247
PsyI GACNNNGTC 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 5 cut(s) 203, 216, 314, 548, 878
RsaNI GTAC 5 cut(s) 202, 215, 313, 547, 877
RseI CAYNNNNRTG 2 cut(s) 1037, 1107
SacII CCGCGG 1 cut(s) 844
SapI GCTCTTC 1 cut(s) 419
SaqAI TTAA 2 cut(s) 1137, 1687
SatI GCNGC 6 cut(s) 193, 383, 713, 903, 1227, 1513
Sau3AI GATC 7 cut(s) 606, 804, 865, 955, 1408, 1485, 1678
Sau96I GGNCC 5 cut(s) 172, 405, 479, 528, 1199
ScaI AGTACT 1 cut(s) 203
SchI GAGTC 2 cut(s) 464, 667
ScrFI CCNGG 4 cut(s) 24, 170, 291, 492
SduI GDGCHC 2 cut(s) 591, 1470
SfaNI GCATC 5 cut(s) 23, 876, 1089, 1405, 1600
SfcI CTRYAG 3 cut(s) 853, 1090, 1248
SfoI GGCGCC 1 cut(s) 489
Sfr303I CCGCGG 1 cut(s) 844
SgrBI CCGCGG 1 cut(s) 844
SinI GGWCC 3 cut(s) 405, 479, 1199
SmiMI CAYNNNNRTG 2 cut(s) 1037, 1107
SmlI CTYRAG 1 cut(s) 1136
SmoI CTYRAG 1 cut(s) 1136
SphI GCATGC 2 cut(s) 1058, 1106
SsiI CCGC 7 cut(s) 190, 193, 383, 461, 712, 841, 843
SspDI GGCGCC 1 cut(s) 487
StyD4I CCNGG 4 cut(s) 22, 168, 289, 490
StyI CCWWGG 3 cut(s) 432, 1035, 1638
TaaI ACNGT 5 cut(s) 184, 402, 754, 1324, 1559
TaqI TCGA 3 cut(s) 303, 814, 1434
TaqII GACCGA 2 cut(s) 261, 467
TatI WGTACW 2 cut(s) 201, 312
TauI GCSGC 3 cut(s) 195, 385, 715
TfiI GAWTC 2 cut(s) 1370, 1628
Tru1I TTAA 2 cut(s) 1137, 1687
Tru9I TTAA 2 cut(s) 1137, 1687
TscAI CASTG 3 cut(s) 710, 1329, 1564
TseFI GTSAC 2 cut(s) 1347, 1547
TseI GCWGC 3 cut(s) 902, 1226, 1512
Tsp45I GTSAC 2 cut(s) 1347, 1547
TspDTI ATGAA 3 cut(s) 800, 1191, 1410
TspRI CASTG 3 cut(s) 710, 1329, 1564
Tth111I GACNNNGTC 1 cut(s) 224
Vha464I CTTAAG 1 cut(s) 1136
VpaK11BI GGWCC 3 cut(s) 405, 479, 1199
XapI RAATTY 2 cut(s) 76, 1439
XceI RCATGY 3 cut(s) 685, 1058, 1106
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
XmiI GTMKAC 2 cut(s) 912, 1026
XmnI GAANNNNTTC 3 cut(s) 969, 1146, 1536
ZrmI AGTACT 1 cut(s) 203
Zsp2I ATGCAT 2 cut(s) 1104, 1615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.