pycom13g08250

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015883
Physical Location & Seq
Reverse (-)
5475467 .. 5478511
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g08250.4

Sequence Viewer

Length: 1290 bp
ATGCCGAGGCCCTGGACCGGCAAAGCTCCGTTGAAGAGCGCCAAGGAGAAGAAGATGAAGAGGAAGGTTCCGCTCTGGGACTCGGTGGGTCCAGTGGTGGCTGGAACCGGTGGAGTGAAGGAAATGGCGAGGCCAATTGAGCTGGGAAAGTACCCCAAGCAGCAAAAATCGAGGGAGGAGATTTTGGGTGATCCATTGAAGAAGTGGGAGATCAAAATGCTTATCAAGCCCCAATTATCCGATAATCGCCAAGTTAATCTCGGAAGAGATGGGTTGACTCATAACATGTTGGACTTGATACATTCGCATTGGAAGCGGAGGCCGGTCTGTAAAGTGAAATGCAAGGGGGTTCCAACTGTGGATATGGATAATGTCTGCCATCATATTGAGGAAAGAACTGGCGGGAAGATCATACATCGAGTTGGCGGTGTGGTTTATCTTTTCCGTGGGAGAAACTATAGCTATACAAATCGCCCGCAATACCCACTGATGCTGTGGAAACCAGCTGCTCCAGTCTACCCAAAACTTATCCAAGAGGCTCCAGAAGGGTTGACAAAAGATGAAGCGGACGTGTTTCGGAAGAAAGGGAAAAGGCTTTTGCCAATATGTAAATTAGCCAAAAATGGAGTATACATTACCTTGGTGAGAGATGTTAGACATGCTTTTGAAGGAAGTCCTTTAGTAAAGATTGACTGTACAGGGATGCATGCAAGTGACTACAAAAAGCTAGGTGCTAAGCTTAAGGAATTGGTTCCGTGCGTGTTACTTTCTTTTGATGATGAACAGATATTGATGTGGAGGGGTTCAAATTGGATATCAATGCATCAATCAGCACCTTCGCCTTCAGAACCAGATGATTTTGATATTCTATTATCAACAGGAAGAGGCAGGGAAGATGATAAACCTTGTAAGCCTGATACCAAAACAGTGATAACAAGCCCAAAAATGATGTCTCTCTGGAAACGCTCGATCGACTCAAACAAAGCACTGCTTCTGGATGAGCTTGACCTCGATCCTGACGCTCTCTTGAAGAAGGTCGAGGAATTCGACAGTGTTTCGCAGGCGACAGAGCACTCCTATCCGGCGCTGATCGTGTCGAGCGAAGACGGTTCCGGCAGCTCGGTTCAAGTGTTTGACGAAGACCAAAGGGACCCTTATAGCGAATACGACGACTATATTGACAATGATTACGATGATGAGAGTGATGATGATGAATTTTATGGGAGTGATTCAATCCCCATGGGATCATTACCTGTCGATGTAATAGCAGAGAAACTGGACCATGATTGA

Protein Analysis

430

Amino Acids

48.55

Weight (kDa)

6.17

Isoelectric Point (pI)

51.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23400
fragaria_vesca FvH4_4g32880
malus_domestica MD13G1094700.v1.1
prunus_persica Prupe.1G250700_v2.0.a1
pyrus_communis pycom13g08250
rosa_chinensis RchiOBHm_Chr4g0441911
rosa_laevigata RLG00000006057
rosa_multiflora Rmu_co8453927.1_g000001
rosa_roxburghii Rroxscaffold_5G00382720
rosa_rugosa Rorug04G0336900
rosa_samantha Rh4AG388600 Rh4BG402300 Rh4CG417400 Rh4DG395600
rosa_wichuraiana Rw4G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 2 cut(s) 516, 630
AciI CCGC 6 cut(s) 71, 316, 402, 426, 476, 566
AclWI GGATC 3 cut(s) 185, 1007, 1252
AcsI RAATTY 2 cut(s) 1043, 1214
AcuI CTGAAG 1 cut(s) 828
AfaI GTAC 2 cut(s) 152, 697
AfiI CCNNNNNNNGG 1 cut(s) 17
AflII CTTAAG 1 cut(s) 740
AflIII ACRYGT 2 cut(s) 285, 570
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 7 cut(s) 34, 199, 668, 807, 1030, 1127, 1233
AjiI CACGTC 1 cut(s) 571
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 8 cut(s) 26, 142, 462, 506, 727, 739, 1003, 1119
AluI AGCT 8 cut(s) 26, 142, 462, 506, 727, 739, 1003, 1119
Alw21I GWGCWC 1 cut(s) 1074
Alw26I GTCTC 1 cut(s) 957
AlwI GGATC 3 cut(s) 185, 1007, 1252
AoxI GGCC 3 cut(s) 8, 131, 320
ApeKI GCWGC 3 cut(s) 160, 506, 1116
ApoI RAATTY 2 cut(s) 1043, 1214
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
AsiGI ACCGGT 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 750
AspLEI GCGC 2 cut(s) 41, 1087
AspS9I GGNCC 5 cut(s) 9, 15, 89, 1150, 1279
AsuHPI GGTGA 2 cut(s) 200, 655
AvaII GGWCC 4 cut(s) 15, 89, 1150, 1279
BbsI GAAGAC 2 cut(s) 1110, 1146
Bbv12I GWGCWC 1 cut(s) 1074
BbvI GCAGC 3 cut(s) 172, 493, 1128
BccI CCATC 2 cut(s) 263, 387
BciT130I CCWGG 1 cut(s) 13
BcoDI GTCTC 1 cut(s) 957
BfaI CTAG 1 cut(s) 728
BfmI CTRYAG 1 cut(s) 457
BfoI RGCGCY 2 cut(s) 42, 1088
BfrI CTTAAG 1 cut(s) 740
BisI GCNGC 3 cut(s) 161, 507, 1117
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 3 cut(s) 162, 508, 1118
Bme1390I CCNGG 1 cut(s) 13
Bme18I GGWCC 4 cut(s) 15, 89, 1150, 1279
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 5 cut(s) 9, 15, 89, 1150, 1279
BmiI GGNNCC 9 cut(s) 69, 90, 106, 351, 540, 753, 1111, 1151, 1152
BmrFI CCNGG 1 cut(s) 13
BmsI GCATC 3 cut(s) 480, 693, 832
BpiI GAAGAC 2 cut(s) 1110, 1146
BpmI CTGGAG 2 cut(s) 495, 525
Bpu1102I GCTNAGC 1 cut(s) 735
BsaJI CCNNGG 6 cut(s) 5, 11, 42, 445, 639, 1239
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse118I RCCGGY 3 cut(s) 17, 107, 322
Bse1I ACTGG 4 cut(s) 92, 403, 512, 1281
BseBI CCWGG 1 cut(s) 13
BseDI CCNNGG 6 cut(s) 5, 11, 42, 445, 639, 1239
BseGI GGATG 2 cut(s) 708, 1003
BseLI CCNNNNNNNGG 1 cut(s) 17
BseNI ACTGG 4 cut(s) 92, 403, 512, 1281
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 3 cut(s) 172, 493, 1128
BseYI CCCAGC 1 cut(s) 142
Bsh1285I CGRYCG 1 cut(s) 972
BshFI GGCC 3 cut(s) 10, 133, 322
BshTI ACCGGT 1 cut(s) 107
BsiEI CGRYCG 1 cut(s) 972
BsiHKAI GWGCWC 1 cut(s) 1074
BsiSI CCGG 5 cut(s) 18, 108, 323, 1082, 1113
BslFI GGGAC 2 cut(s) 92, 1163
BslI CCNNNNNNNGG 1 cut(s) 17
BsmAI GTCTC 1 cut(s) 957
BsmFI GGGAC 2 cut(s) 92, 1163
BsnI GGCC 3 cut(s) 10, 133, 322
Bsp1286I GDGCHC 1 cut(s) 1074
Bsp1407I TGTACA 1 cut(s) 695
Bsp143I GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
Bsp1720I GCTNAGC 1 cut(s) 735
Bsp19I CCATGG 1 cut(s) 1239
BspACI CCGC 6 cut(s) 71, 316, 402, 426, 476, 566
BspANI GGCC 3 cut(s) 10, 133, 322
BspLI GGNNCC 9 cut(s) 69, 90, 106, 351, 540, 753, 1111, 1151, 1152
BspPI GGATC 3 cut(s) 185, 1007, 1252
BspQI GCTCTTC 1 cut(s) 29
BspTI CTTAAG 1 cut(s) 740
BsrBI CCGCTC 1 cut(s) 73
BsrFI RCCGGY 3 cut(s) 17, 107, 322
BsrGI TGTACA 1 cut(s) 695
BsrI ACTGG 4 cut(s) 92, 403, 512, 1281
BssAI RCCGGY 3 cut(s) 17, 107, 322
BssECI CCNNGG 6 cut(s) 5, 11, 42, 445, 639, 1239
BssMI GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
BssNAI GTATAC 1 cut(s) 631
BssT1I CCWWGG 3 cut(s) 42, 639, 1239
Bst1107I GTATAC 1 cut(s) 631
Bst2UI CCWGG 1 cut(s) 13
Bst4CI ACNGT 5 cut(s) 358, 695, 928, 1052, 1109
Bst6I CTCTTC 4 cut(s) 29, 53, 259, 877
BstAFI CTTAAG 1 cut(s) 740
BstAUI TGTACA 1 cut(s) 695
BstC8I GCNNGC 3 cut(s) 476, 708, 1062
BstDEI CTNAG 1 cut(s) 735
BstDSI CCRYGG 2 cut(s) 445, 1239
BstF5I GGATG 2 cut(s) 708, 1003
BstH2I RGCGCY 2 cut(s) 42, 1088
BstHHI GCGC 2 cut(s) 41, 1087
BstKTI GATC 7 cut(s) 193, 213, 411, 972, 1015, 1092, 1247
BstMAI GTCTC 1 cut(s) 957
BstMBI GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
BstMCI CGRYCG 1 cut(s) 972
BstMWI GCNNNNNNNGC 3 cut(s) 139, 226, 313
BstNI CCWGG 1 cut(s) 13
BstNSI RCATGY 3 cut(s) 289, 662, 710
BstSCI CCNGG 1 cut(s) 11
BstSFI CTRYAG 1 cut(s) 457
BstV1I GCAGC 3 cut(s) 172, 493, 1128
BstV2I GAAGAC 2 cut(s) 1110, 1146
BstZ17I GTATAC 1 cut(s) 631
BsuRI GGCC 3 cut(s) 10, 133, 322
BtgI CCRYGG 2 cut(s) 445, 1239
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 2 cut(s) 708, 1003
BtsI GCAGTG 1 cut(s) 986
BtsIMutI CAGTG 5 cut(s) 99, 485, 933, 986, 1057
Cac8I GCNNGC 3 cut(s) 476, 708, 1062
CfoI GCGC 2 cut(s) 41, 1087
Cfr10I RCCGGY 3 cut(s) 17, 107, 322
Cfr13I GGNCC 5 cut(s) 9, 15, 89, 1150, 1279
CseI GACGC 1 cut(s) 1028
Csp6I GTAC 2 cut(s) 151, 696
CspAI ACCGGT 1 cut(s) 107
CviAII CATG 5 cut(s) 286, 659, 707, 1240, 1283
CviQI GTAC 2 cut(s) 151, 696
DdeI CTNAG 1 cut(s) 735
DpnI GATC 7 cut(s) 192, 212, 410, 971, 1014, 1091, 1246
DpnII GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
Eam1104I CTCTTC 4 cut(s) 29, 53, 259, 877
EarI CTCTTC 4 cut(s) 29, 53, 259, 877
Eco130I CCWWGG 3 cut(s) 42, 639, 1239
Eco32I GATATC 1 cut(s) 816
Eco47I GGWCC 4 cut(s) 15, 89, 1150, 1279
Eco57I CTGAAG 1 cut(s) 828
EcoO109I RGGNCCY 2 cut(s) 9, 1150
EcoRI GAATTC 1 cut(s) 1043
EcoRII CCWGG 1 cut(s) 11
EcoRV GATATC 1 cut(s) 816
EcoT14I CCWWGG 3 cut(s) 42, 639, 1239
EcoT22I ATGCAT 2 cut(s) 708, 825
ErhI CCWWGG 3 cut(s) 42, 639, 1239
FaeI CATG 5 cut(s) 289, 662, 710, 1243, 1286
FalI AAGNNNNNCTT 4 cut(s) 975, 1007, 1138, 1170
FaqI GGGAC 2 cut(s) 92, 1163
FatI CATG 5 cut(s) 285, 658, 706, 1239, 1282
FauI CCCGC 2 cut(s) 395, 483
FblI GTMKAC 2 cut(s) 516, 630
Fnu4HI GCNGC 3 cut(s) 161, 507, 1117
FokI GGATG 2 cut(s) 715, 1010
Fsp4HI GCNGC 3 cut(s) 161, 507, 1117
FspBI CTAG 1 cut(s) 728
GlaI GCGC 2 cut(s) 40, 1086
GluI GCNGC 3 cut(s) 161, 507, 1117
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 2 cut(s) 495, 525
HaeII RGCGCY 2 cut(s) 42, 1088
HaeIII GGCC 3 cut(s) 10, 133, 322
HapII CCGG 5 cut(s) 18, 108, 323, 1082, 1113
HgaI GACGC 1 cut(s) 1028
HhaI GCGC 2 cut(s) 41, 1087
Hin1II CATG 5 cut(s) 289, 662, 710, 1243, 1286
Hin6I GCGC 2 cut(s) 39, 1085
HinP1I GCGC 2 cut(s) 39, 1085
HincII GTYRAC 2 cut(s) 276, 552
HindII GTYRAC 2 cut(s) 276, 552
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 4 cut(s) 80, 277, 974, 1229
HpaII CCGG 5 cut(s) 18, 108, 323, 1082, 1113
HphI GGTGA 2 cut(s) 200, 655
Hpy166II GTNNAC 4 cut(s) 276, 517, 552, 631
Hpy188I TCNGA 4 cut(s) 241, 263, 579, 847
Hpy188III TCNNGA 5 cut(s) 542, 958, 995, 1016, 1027
Hpy8I GTNNAC 4 cut(s) 276, 517, 552, 631
Hpy99I CGWCG 1 cut(s) 1172
HpyAV CCTTC 7 cut(s) 58, 112, 539, 662, 846, 852, 1027
HpyCH4III ACNGT 5 cut(s) 358, 695, 928, 1052, 1109
HpyCH4IV ACGT 1 cut(s) 570
HpyCH4V TGCA 4 cut(s) 342, 706, 710, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 226, 313
HpyF3I CTNAG 1 cut(s) 735
HpySE526I ACGT 1 cut(s) 570
Hsp92II CATG 5 cut(s) 289, 662, 710, 1243, 1286
HspAI GCGC 2 cut(s) 39, 1085
KflI GGGWCCC 1 cut(s) 1150
Kzo9I GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
LguI GCTCTTC 1 cut(s) 29
LmnI GCTCC 3 cut(s) 31, 514, 544
Lsp1109I GCAGC 3 cut(s) 172, 493, 1128
LweI GCATC 3 cut(s) 480, 693, 832
MaeI CTAG 1 cut(s) 728
MaeII ACGT 1 cut(s) 570
MaeIII GTNAC 2 cut(s) 713, 762
MalI GATC 7 cut(s) 192, 212, 410, 971, 1014, 1091, 1246
MbiI CCGCTC 1 cut(s) 73
MboI GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
MfeI CAATTG 1 cut(s) 135
MhlI GDGCHC 1 cut(s) 1074
MluCI AATT 7 cut(s) 135, 233, 611, 746, 808, 1043, 1214
MlyI GAGTC 3 cut(s) 74, 271, 968
MmeI TCCRAC 2 cut(s) 270, 377
Mph1103I ATGCAT 2 cut(s) 708, 825
MroXI GAANNNNTTC 1 cut(s) 750
MseI TTAA 2 cut(s) 255, 741
MslI CAYNNNNRTG 1 cut(s) 711
MspA1I CMGCKG 1 cut(s) 506
MspCI CTTAAG 1 cut(s) 740
MspI CCGG 5 cut(s) 18, 108, 323, 1082, 1113
MspR9I CCNGG 1 cut(s) 13
MunI CAATTG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 13
MwoI GCNNNNNNNGC 3 cut(s) 139, 226, 313
NcoI CCATGG 1 cut(s) 1239
NdeII GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
NlaIII CATG 5 cut(s) 289, 662, 710, 1243, 1286
NlaIV GGNNCC 9 cut(s) 69, 90, 106, 351, 540, 753, 1111, 1151, 1152
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 713
NsiI ATGCAT 2 cut(s) 708, 825
NspI RCATGY 3 cut(s) 289, 662, 710
PaeI GCATGC 1 cut(s) 710
PciI ACATGT 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 29
PcsI WCGNNNNNNNCGW 2 cut(s) 1044, 1098
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 1229
PinAI ACCGGT 1 cut(s) 107
PkrI GCNGC 3 cut(s) 162, 508, 1118
Ple19I CGATCG 1 cut(s) 972
PleI GAGTC 3 cut(s) 74, 271, 968
PpsI GAGTC 3 cut(s) 74, 271, 968
PpuMI RGGWCCY 1 cut(s) 1150
PscI ACATGT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 1150
Psp6I CCWGG 1 cut(s) 11
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 11
PspN4I GGNNCC 9 cut(s) 69, 90, 106, 351, 540, 753, 1111, 1151, 1152
PspPI GGNCC 5 cut(s) 9, 15, 89, 1150, 1279
PspPPI RGGWCCY 1 cut(s) 1150
PvuI CGATCG 1 cut(s) 972
PvuII CAGCTG 1 cut(s) 506
RsaI GTAC 2 cut(s) 152, 697
RsaNI GTAC 2 cut(s) 151, 696
RseI CAYNNNNRTG 1 cut(s) 711
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 255, 741
SatI GCNGC 3 cut(s) 161, 507, 1117
Sau3AI GATC 7 cut(s) 190, 210, 408, 969, 1012, 1089, 1244
Sau96I GGNCC 5 cut(s) 9, 15, 89, 1150, 1279
SchI GAGTC 3 cut(s) 74, 271, 968
ScrFI CCNGG 1 cut(s) 13
SduI GDGCHC 1 cut(s) 1074
SfaNI GCATC 3 cut(s) 480, 693, 832
SfcI CTRYAG 1 cut(s) 457
SinI GGWCC 4 cut(s) 15, 89, 1150, 1279
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
SphI GCATGC 1 cut(s) 710
Sse9I AATT 7 cut(s) 135, 233, 611, 746, 808, 1043, 1214
SsiI CCGC 6 cut(s) 71, 316, 402, 426, 476, 566
SspMI CTAG 1 cut(s) 728
StyD4I CCNGG 1 cut(s) 11
StyI CCWWGG 3 cut(s) 42, 639, 1239
TaaI ACNGT 5 cut(s) 358, 695, 928, 1052, 1109
TaiI ACGT 1 cut(s) 573
TaqI TCGA 9 cut(s) 170, 418, 968, 972, 1011, 1038, 1047, 1097, 1257
TasI AATT 7 cut(s) 135, 233, 611, 746, 808, 1043, 1214
TatI WGTACW 1 cut(s) 695
TfiI GAWTC 1 cut(s) 1229
Tru1I TTAA 2 cut(s) 255, 741
Tru9I TTAA 2 cut(s) 255, 741
TscAI CASTG 5 cut(s) 99, 492, 933, 993, 1057
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 3 cut(s) 160, 506, 1116
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 4 cut(s) 71, 576, 795, 1227
TspGWI ACGGA 3 cut(s) 18, 434, 744
TspRI CASTG 5 cut(s) 99, 492, 933, 993, 1057
Vha464I CTTAAG 1 cut(s) 740
VpaK11BI GGWCC 4 cut(s) 15, 89, 1150, 1279
XapI RAATTY 2 cut(s) 1043, 1214
XceI RCATGY 3 cut(s) 289, 662, 710
XcmI CCANNNNNNNNNTGG 2 cut(s) 201, 492
XmiI GTMKAC 2 cut(s) 516, 630
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 1 cut(s) 728
Zsp2I ATGCAT 2 cut(s) 708, 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.