Rorug04G0336900

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002061
Physical Location & Seq
Reverse (-)
51067793 .. 51070163
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0336900.1

Sequence Viewer

Length: 735 bp
ATGTGGCAAGATCAGCCCAGAAAGTCTTGTTTCGGAGAATCTATCAAGGCTCTTGAAGCTGATATACAACATGCCAATAGCTTGGCAGCTTCTCTTCCCAGAGATTATGGTGGGAATTCTATCCAAATGAGATTGTCTTACAGCCCCTTTGCCCCTCTTTTCCTTTATTTCATTGAATGGATGGATTATAGTTGCACTGATATACTTCCCAATTACTTGGGCCTCCTCCACATACTTGTATACAAGGTATATGTTGATGGGGTTCCTTCAATATCCTCCAAAGAACAAAAGGTCACTATAAGGGAATTTTATGCTGTAATATACCCTTATCTTAGACAACTTGAAGGCGAGTTTAACGAACTGGAAGATAATAAGAAGAGAAGCCAAAAGAGGATGGAAGACAGGAAAAACCTCTCTGATCAGGATCTAGAAAGAGATGATGAATGTGGTATATGTATGGAAAACTATGCAAAGATGGTGTTGCCGAATTGTGGGCATTCTATGTGCATCACCTGCTTCCATGATTGGAATGTGCGATCACAGTCCTGCCCTTTTTGCCGTGGCAACCTCAAGAGAGTTAGCTCTACAGATTTGTGGGTACTCACAAGAAACAGCGACGTGATTGATGCTGTAACCCTTGCAAAAGAGAACCTTAGACGCTTCTATCTTTATGTTGAAAATATTCCTGTTGTTATGCCAGCAACACATGTTGTGGTATATGATTACATGCTCTGA

Protein Analysis

244

Amino Acids

28.44

Weight (kDa)

5.59

Isoelectric Point (pI)

65.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 146 - 192 1.1e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 147 - 187 7.3e-09 Ring finger domain
zf-RING_5 PF14634 148 - 187 1.4e-06 zinc-RING finger domain
zf-C3HC4_2 PF13923 148 - 186 4.7e-06 Zinc finger, C3HC4 type (RING finger)
Prok-RING_4 PF14447 149 - 191 1.1e-06 Prokaryotic RING finger family 4
zf-C3HC4 PF00097 149 - 186 2.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 521
AccI GTMKAC 1 cut(s) 240
AclWI GGATC 1 cut(s) 432
AcsI RAATTY 2 cut(s) 115, 305
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 706
AgsI TTSAA 5 cut(s) 56, 176, 270, 344, 677
AjiI CACGTC 1 cut(s) 619
AluBI AGCT 4 cut(s) 59, 81, 89, 582
AluI AGCT 4 cut(s) 59, 81, 89, 582
AlwI GGATC 1 cut(s) 432
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 2 cut(s) 115, 305
Asp700I GAANNNNTTC 1 cut(s) 681
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 502
BarI GAAGNNNNNNTAC 2 cut(s) 48, 80
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 98
BccI CCATC 4 cut(s) 175, 251, 388, 469
BceAI ACGGC 1 cut(s) 543
BclI TGATCA 1 cut(s) 418
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 521
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 264
BmsI GCATC 2 cut(s) 516, 616
BpiI GAAGAC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 554
BsaBI GATNNNNATC 1 cut(s) 423
BsaJI CCNNGG 1 cut(s) 559
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse1I ACTGG 1 cut(s) 366
Bse8I GATNNNNATC 1 cut(s) 423
BseDI CCNNGG 1 cut(s) 559
BseGI GGATG 2 cut(s) 186, 399
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 491
BseNI ACTGG 1 cut(s) 366
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 1 cut(s) 98
BshFI GGCC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 491
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 1 cut(s) 222
Bsp143I GATC 4 cut(s) 10, 418, 424, 536
BspANI GGCC 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 521
BspPI GGATC 1 cut(s) 432
BsrI ACTGG 1 cut(s) 366
BssECI CCNNGG 1 cut(s) 559
BssMI GATC 4 cut(s) 10, 418, 424, 536
BssNAI GTATAC 1 cut(s) 241
Bst1107I GTATAC 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 543
Bst6I CTCTTC 2 cut(s) 99, 371
BstAPI GCANNNNNTGC 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 2 cut(s) 332, 653
BstDSI CCRYGG 1 cut(s) 559
BstF5I GGATG 2 cut(s) 186, 399
BstKTI GATC 4 cut(s) 13, 421, 427, 539
BstMBI GATC 4 cut(s) 10, 418, 424, 536
BstMWI GCNNNNNNNGC 4 cut(s) 13, 56, 513, 555
BstNSI RCATGY 3 cut(s) 74, 710, 730
BstSFI CTRYAG 1 cut(s) 585
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 424
BstXI CCANNNNNNTGG 2 cut(s) 82, 217
BstYI RGATCY 1 cut(s) 424
BstZ17I GTATAC 1 cut(s) 241
BsuRI GGCC 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 559
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 2 cut(s) 186, 399
BtsIMutI CAGTG 1 cut(s) 195
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 699
Cfr13I GGNCC 1 cut(s) 220
CseI GACGC 1 cut(s) 666
Csp6I GTAC 1 cut(s) 599
CviAII CATG 4 cut(s) 71, 521, 707, 727
CviJI RGCY 9 cut(s) 16, 50, 59, 81, 89, 144, 222, 384, 582
CviKI_1 RGCY 9 cut(s) 16, 50, 59, 81, 89, 144, 222, 384, 582
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 2 cut(s) 332, 653
DpnI GATC 4 cut(s) 12, 420, 426, 538
DpnII GATC 4 cut(s) 10, 418, 424, 536
Eam1104I CTCTTC 2 cut(s) 99, 371
EarI CTCTTC 2 cut(s) 99, 371
EcoRI GAATTC 1 cut(s) 115
FaeI CATG 4 cut(s) 74, 524, 710, 730
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FatI CATG 4 cut(s) 70, 520, 706, 726
FbaI TGATCA 1 cut(s) 418
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 2 cut(s) 193, 406
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 1 cut(s) 428
GluI GCNGC 1 cut(s) 87
HaeIII GGCC 1 cut(s) 222
HgaI GACGC 1 cut(s) 666
Hin1II CATG 4 cut(s) 74, 524, 710, 730
HinfI GANTC 1 cut(s) 38
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 3 cut(s) 35, 418, 734
Hpy188III TCNNGA 4 cut(s) 53, 422, 428, 571
Hpy8I GTNNAC 1 cut(s) 241
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 2 cut(s) 276, 338
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 4 cut(s) 195, 470, 507, 641
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 56, 513, 555
HpyF3I CTNAG 2 cut(s) 332, 653
HpySE526I ACGT 1 cut(s) 618
Hsp92II CATG 4 cut(s) 74, 524, 710, 730
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 4 cut(s) 10, 418, 424, 536
LpnPI CCDG 9 cut(s) 31, 112, 347, 388, 407, 526, 559, 699, 711
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 2 cut(s) 516, 616
MaeI CTAG 1 cut(s) 428
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 2 cut(s) 292, 631
MalI GATC 4 cut(s) 12, 420, 426, 538
MboI GATC 4 cut(s) 10, 418, 424, 536
MboII GAAGA 4 cut(s) 86, 377, 388, 410
MflI RGATCY 1 cut(s) 424
MluCI AATT 4 cut(s) 115, 211, 305, 487
MnlI CCTC 7 cut(s) 165, 233, 236, 286, 384, 422, 578
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 496
MwoI GCNNNNNNNGC 4 cut(s) 13, 56, 513, 555
NdeII GATC 4 cut(s) 10, 418, 424, 536
NlaIII CATG 4 cut(s) 74, 524, 710, 730
NlaIV GGNNCC 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 292
NspI RCATGY 3 cut(s) 74, 710, 730
PaqCI CACCTGC 1 cut(s) 521
PciI ACATGT 1 cut(s) 706
PctI GAATGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 1 cut(s) 38
PkrI GCNGC 1 cut(s) 88
PscI ACATGT 1 cut(s) 706
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 220
PsuI RGATCY 1 cut(s) 424
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 4 cut(s) 10, 418, 424, 536
Sau96I GGNCC 1 cut(s) 220
SfaNI GCATC 2 cut(s) 516, 616
SfcI CTRYAG 1 cut(s) 585
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 4 cut(s) 115, 211, 305, 487
SspI AATATT 1 cut(s) 682
SspMI CTAG 1 cut(s) 428
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 1 cut(s) 621
TasI AATT 4 cut(s) 115, 211, 305, 487
TfiI GAWTC 1 cut(s) 38
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 160, 456
TspRI CASTG 1 cut(s) 202
XapI RAATTY 2 cut(s) 115, 305
XbaI TCTAGA 1 cut(s) 427
XceI RCATGY 3 cut(s) 74, 710, 730
XmiI GTMKAC 1 cut(s) 240
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.