Rh5BG501200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002061
Physical Location & Seq
Forward (+)
79765923 .. 79769677
3755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG501200.1

Sequence Viewer

Length: 699 bp
ATGACCACGGATACTAATGTTCTAAGCTATTGGAACAACTGGAGGTTCTTGATCTGTGCAATATTTATATTGAGTAGTATCGTGCTAGCCGCAGTTCTTATATGGAAATATGAAGGCTGCAAAAAATCCAGAAGGGAAAGAAGAGAAAATCAGCGCGAAACAGTAGGATCTCTATATGAGGATGAAGCCTGGAGGACATCTCTCAAAGTAATTCATCCCGCTTGGCTTCTTGCTTACAGATTAATTGCTTTCGTTGTGCTTCTATCCTTGATAGTTACCAATATTGTTCTTGATGGAGTTGGCATACTTTACTTCTACACTCAGTGGACATTTTGTTTGGTTACAATCTATTTTGGGCTTGGATCTTCACTCTCCATTTATGGATGCTACAAATACCGCAGTAGAGTTGGTGGGGATGCAGCTGATCATGTGAGAGATGAAGAGCGAGGTACTTATGTTGCTCCAACACTTGGGGAAAATGCAGATACATCCAACACATATAAAAGTTTTACTGATGAAGAATGTCCCTCTCGCAAAGGCGCTGGTGTATATGTTTATATCTTTCAAGTCATTTATCAGATGTGTGGGGGTGCTGTGGTGCTCACAGATGTTATATTTTGGCTCATTTTGTATCCATTTCTAACAAGCAAAGATTACAAACTGAACTTTGTAAGTATACTGCCGTTTCAATGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

232

Amino Acids

26.72

Weight (kDa)

7.54

Isoelectric Point (pI)

26.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 470
AccI GTMKAC 1 cut(s) 676
AccII CGCG 1 cut(s) 156
AciI CCGC 3 cut(s) 90, 219, 397
AclWI GGATC 2 cut(s) 175, 370
AdeI CACNNNGTG 1 cut(s) 324
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 2 cut(s) 566, 689
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 2 cut(s) 27, 422
AluI AGCT 2 cut(s) 27, 422
Alw21I GWGCWC 1 cut(s) 603
AlwI GGATC 2 cut(s) 175, 370
ApeKI GCWGC 2 cut(s) 117, 419
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AseI ATTAAT 1 cut(s) 242
AspLEI GCGC 2 cut(s) 156, 542
AspS9I GGNCC 1 cut(s) 693
AsuNHI GCTAGC 1 cut(s) 85
AvaII GGWCC 1 cut(s) 693
BaeI ACNNNNGTAYC 3 cut(s) 36, 441, 474
Bbv12I GWGCWC 1 cut(s) 603
BbvI GCAGC 2 cut(s) 104, 431
BccI CCATC 1 cut(s) 287
BceAI ACGGC 1 cut(s) 667
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 2 cut(s) 4, 642
BclI TGATCA 1 cut(s) 424
BfaI CTAG 1 cut(s) 86
BfoI RGCGCY 1 cut(s) 543
BfuI GTATCC 2 cut(s) 4, 642
BisI GCNGC 3 cut(s) 90, 118, 420
BlsI GCNGC 3 cut(s) 91, 119, 421
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 693
BmgT120I GGNCC 1 cut(s) 693
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 2 cut(s) 374, 406
BmtI GCTAGC 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpmI CTGGAG 2 cut(s) 61, 211
BsaJI CCNNGG 1 cut(s) 6
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 44
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 5 cut(s) 187, 214, 389, 421, 488
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 104, 431
Bsh1236I CGCG 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 603
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 603
Bsp143I GATC 4 cut(s) 51, 167, 362, 424
BspACI CCGC 3 cut(s) 90, 219, 397
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 1 cut(s) 156
BspOI GCTAGC 1 cut(s) 89
BspPI GGATC 2 cut(s) 175, 370
BspQI GCTCTTC 1 cut(s) 435
BsrI ACTGG 1 cut(s) 44
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 4 cut(s) 51, 167, 362, 424
BssNAI GTATAC 1 cut(s) 677
Bst1107I GTATAC 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 2 cut(s) 136, 435
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 2 cut(s) 23, 321
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 5 cut(s) 187, 214, 389, 421, 488
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 2 cut(s) 156, 542
BstKTI GATC 4 cut(s) 54, 170, 365, 427
BstMBI GATC 4 cut(s) 51, 167, 362, 424
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 188
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 2 cut(s) 104, 431
BstX2I RGATCY 2 cut(s) 167, 362
BstYI RGATCY 2 cut(s) 167, 362
BstZ17I GTATAC 1 cut(s) 677
BsuI GTATCC 2 cut(s) 4, 642
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 5 cut(s) 187, 214, 389, 421, 488
BtsIMutI CAGTG 1 cut(s) 329
Cac8I GCNNGC 1 cut(s) 87
CfoI GCGC 2 cut(s) 156, 542
Cfr13I GGNCC 1 cut(s) 693
Csp6I GTAC 1 cut(s) 450
CviAII CATG 1 cut(s) 428
CviJI RGCY 8 cut(s) 27, 89, 117, 188, 226, 358, 422, 622
CviKI_1 RGCY 8 cut(s) 27, 89, 117, 188, 226, 358, 422, 622
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 2 cut(s) 23, 321
DpnI GATC 4 cut(s) 53, 169, 364, 426
DpnII GATC 4 cut(s) 51, 167, 362, 424
DraIII CACNNNGTG 1 cut(s) 324
Eam1104I CTCTTC 2 cut(s) 136, 435
EarI CTCTTC 2 cut(s) 136, 435
Eco47I GGWCC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 188
FaeI CATG 1 cut(s) 431
FaqI GGGAC 1 cut(s) 510
FatI CATG 1 cut(s) 427
FauI CCCGC 1 cut(s) 226
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 1 cut(s) 676
Fnu4HI GCNGC 3 cut(s) 90, 118, 420
FokI GGATG 5 cut(s) 194, 201, 396, 428, 475
Fsp4HI GCNGC 3 cut(s) 90, 118, 420
FspBI CTAG 1 cut(s) 86
GlaI GCGC 2 cut(s) 155, 541
GluI GCNGC 3 cut(s) 90, 118, 420
GsuI CTGGAG 2 cut(s) 61, 211
HaeII RGCGCY 1 cut(s) 543
HhaI GCGC 2 cut(s) 156, 542
Hin1II CATG 1 cut(s) 431
Hin6I GCGC 2 cut(s) 154, 540
HinP1I GCGC 2 cut(s) 154, 540
Hpy166II GTNNAC 2 cut(s) 327, 677
Hpy188I TCNGA 1 cut(s) 579
Hpy188III TCNNGA 3 cut(s) 49, 129, 290
Hpy8I GTNNAC 2 cut(s) 327, 677
HpyAV CCTTC 2 cut(s) 107, 126
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4V TGCA 4 cut(s) 59, 120, 419, 482
HpyF3I CTNAG 2 cut(s) 23, 321
Hsp92II CATG 1 cut(s) 431
HspAI GCGC 2 cut(s) 154, 540
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 4 cut(s) 51, 167, 362, 424
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 1 cut(s) 466
LpnPI CCDG 5 cut(s) 25, 142, 175, 202, 528
Lsp1109I GCAGC 2 cut(s) 104, 431
LweI GCATC 2 cut(s) 374, 406
MaeI CTAG 1 cut(s) 86
MaeIII GTNAC 2 cut(s) 274, 340
MalI GATC 4 cut(s) 53, 169, 364, 426
MboI GATC 4 cut(s) 51, 167, 362, 424
MboII GAAGA 4 cut(s) 153, 357, 452, 530
MflI RGATCY 2 cut(s) 167, 362
MhlI GDGCHC 1 cut(s) 603
MluCI AATT 2 cut(s) 210, 243
MmeI TCCRAC 2 cut(s) 488, 516
MnlI CCTC 5 cut(s) 36, 172, 186, 440, 538
MseI TTAA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 156
NdeII GATC 4 cut(s) 51, 167, 362, 424
NheI GCTAGC 1 cut(s) 85
NlaIII CATG 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 435
PflMI CCANNNNNTGG 1 cut(s) 470
PkrI GCNGC 3 cut(s) 91, 119, 421
PshBI ATTAAT 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspPI GGNCC 1 cut(s) 693
PsuI RGATCY 2 cut(s) 167, 362
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 3 cut(s) 90, 118, 420
Sau3AI GATC 4 cut(s) 51, 167, 362, 424
Sau96I GGNCC 1 cut(s) 693
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 603
SetI ASST 5 cut(s) 29, 47, 424, 451, 698
SfaNI GCATC 2 cut(s) 374, 406
SinI GGWCC 1 cut(s) 693
Sse9I AATT 2 cut(s) 210, 243
SsiI CCGC 3 cut(s) 90, 219, 397
SspI AATATT 2 cut(s) 63, 283
SspMI CTAG 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 163
TasI AATT 2 cut(s) 210, 243
TauI GCSGC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 1 cut(s) 329
TseI GCWGC 2 cut(s) 117, 419
TspDTI ATGAA 5 cut(s) 126, 198, 203, 453, 531
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 693
VspI ATTAAT 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 676
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.