Rw4G033580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002061
Physical Location & Seq
Forward (+)
60702232 .. 60704365
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033580.1

Sequence Viewer

Length: 996 bp
ATGACTACGGATACTACGGTTCTAAGGTATTGGTGGAACTGGAGGTTCTTGATCAGTGCAATATTTATATTGTCTAGTATGGTTCTAGCCGCAGTTCTTATATGGAAATATGAAGGCTGCAAAAAATCCAGAAGGGGAAGAAGAGAAAATCAGCGAGAAACAGTAGGATCTCTATTTGAGGATGAAGCCTGGAGGACATCTTTCAAATTAATTCATCCCACTTGGCTTCTTGCTTATAGAGTTGTTGCTTTTGTTTTGCTTCTGTCCTTGATAGTTACCAATTTTGTTCTTGATGGAGCTGGCATACTTTACTTCTATACTCAGTGGACTTTTTGTTTGGTCACAATCTATTTTGGGCTTGGATCTTCACTCTCCATTTATGGATGCTACAAATACCGCAATAGAGTTGGTGAGGATGCAGCTGATTTTGTGAGACATGAAGAACGAGGTAGTACTTATGTTGCTCCAACACTTGGCAAAAATGCAGATACATCCAACTCATCTAAAAGTTTCACTGACGAAGAATGTCCCTCCCACAAAGGCGCTGGTCTGATTGTTTATATCTTTCAAGTCATTTATCAGATGTGTGGGGGTGCTGTGGTGCTCACTGATGTGGTATTTTGGCTCATTCTGTATCCATTTCTAACAAGCAAAGATTACAAACTGGACTTTATGATGGCCAGTATGCACTCATTCAATCTTGTTTTCCTCCTTGGTGAGACAACTTTGAATAGCTTGCAGTTTCCATTGTTTCGAATCTCATATTTTGTGCTATGGACGGGAGTATTCGTGATTTTCCAATGGACAATCCACGCTTGTAAAAACATGTGGTGGCCTTATCCATTTCTCGACTTGTCATCCTCATATGCACCCTTATGGTATCTTGGGGTTGGAGTGATGCATATCCCATGCTACGGTATCTTTGCTTTAATTATTTGGATGAAGCAGTTGTGGTTCTCAAGATCATTACCGGAGTCTTTTCAGGGTGAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

38.5

Weight (kDa)

8.58

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 473
AciI CCGC 2 cut(s) 90, 397
AclWI GGATC 2 cut(s) 175, 370
AcoI YGGCCR 1 cut(s) 678
AfaI GTAC 1 cut(s) 454
AfiI CCNNNNNNNGG 2 cut(s) 473, 914
AflIII ACRYGT 1 cut(s) 825
AgsI TTSAA 4 cut(s) 205, 569, 697, 730
AjnI CCWGG 1 cut(s) 188
AleI CACNNNNGTG 1 cut(s) 611
AluBI AGCT 3 cut(s) 299, 422, 735
AluI AGCT 3 cut(s) 299, 422, 735
Alw21I GWGCWC 1 cut(s) 606
Alw26I GTCTC 2 cut(s) 427, 713
AlwI GGATC 2 cut(s) 175, 370
AoxI GGCC 2 cut(s) 678, 833
ApeKI GCWGC 2 cut(s) 117, 419
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AseI ATTAAT 1 cut(s) 209
AspLEI GCGC 1 cut(s) 545
AsuHPI GGTGA 2 cut(s) 422, 728
AsuII TTCGAA 1 cut(s) 754
BaeI ACNNNNGTAYC 1 cut(s) 36
BalI TGGCCA 1 cut(s) 680
Bbv12I GWGCWC 1 cut(s) 606
BbvI GCAGC 2 cut(s) 104, 431
BccI CCATC 2 cut(s) 287, 670
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 2 cut(s) 4, 645
BclI TGATCA 1 cut(s) 51
BcoDI GTCTC 2 cut(s) 427, 713
BfaI CTAG 2 cut(s) 75, 86
BfoI RGCGCY 1 cut(s) 546
BfuI GTATCC 2 cut(s) 4, 645
BisI GCNGC 3 cut(s) 90, 118, 420
BlsI GCNGC 3 cut(s) 91, 119, 421
BmcAI AGTACT 1 cut(s) 454
Bme1390I CCNGG 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 190
BmsI GCATC 3 cut(s) 374, 406, 888
BpmI CTGGAG 2 cut(s) 61, 211
Bpu14I TTCGAA 1 cut(s) 754
BpuEI CTTGAG 1 cut(s) 943
BsaBI GATNNNNATC 1 cut(s) 902
BsaJI CCNNGG 1 cut(s) 712
BsaWI WCCGGW 1 cut(s) 970
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bsc4I CCNNNNNNNGG 2 cut(s) 473, 914
Bse1I ACTGG 3 cut(s) 44, 669, 681
Bse8I GATNNNNATC 1 cut(s) 902
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 712
BseGI GGATG 7 cut(s) 187, 214, 389, 421, 491, 857, 945
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 2 cut(s) 473, 914
BseMII CTCAG 1 cut(s) 335
BseNI ACTGG 3 cut(s) 44, 669, 681
BseXI GCAGC 2 cut(s) 104, 431
BshFI GGCC 2 cut(s) 680, 835
BsiHKAI GWGCWC 1 cut(s) 606
BsiSI CCGG 1 cut(s) 971
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 2 cut(s) 473, 914
BsmAI GTCTC 2 cut(s) 427, 713
BsmFI GGGAC 1 cut(s) 513
BsnI GGCC 2 cut(s) 680, 835
Bsp119I TTCGAA 1 cut(s) 754
Bsp1286I GDGCHC 1 cut(s) 606
Bsp143I GATC 4 cut(s) 51, 167, 362, 962
BspACI CCGC 2 cut(s) 90, 397
BspANI GGCC 2 cut(s) 680, 835
BspCNI CTCAG 1 cut(s) 334
BspPI GGATC 2 cut(s) 175, 370
BspT104I TTCGAA 1 cut(s) 754
BsrI ACTGG 3 cut(s) 44, 669, 681
BssECI CCNNGG 1 cut(s) 712
BssMI GATC 4 cut(s) 51, 167, 362, 962
BssT1I CCWWGG 1 cut(s) 712
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 3 cut(s) 19, 163, 917
Bst6I CTCTTC 1 cut(s) 136
BstBI TTCGAA 1 cut(s) 754
BstC8I GCNNGC 2 cut(s) 301, 737
BstDEI CTNAG 2 cut(s) 23, 321
BstF5I GGATG 7 cut(s) 187, 214, 389, 421, 491, 857, 945
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 4 cut(s) 54, 170, 365, 965
BstMAI GTCTC 2 cut(s) 427, 713
BstMBI GATC 4 cut(s) 51, 167, 362, 962
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 1 cut(s) 188
BstV1I GCAGC 2 cut(s) 104, 431
BstX2I RGATCY 2 cut(s) 167, 362
BstYI RGATCY 2 cut(s) 167, 362
BsuI GTATCC 2 cut(s) 4, 645
BsuRI GGCC 2 cut(s) 680, 835
BtsCI GGATG 7 cut(s) 187, 214, 389, 421, 491, 857, 945
BtsIMutI CAGTG 4 cut(s) 61, 329, 513, 606
Cac8I GCNNGC 2 cut(s) 301, 737
CfoI GCGC 1 cut(s) 545
Csp6I GTAC 1 cut(s) 453
CviAII CATG 3 cut(s) 437, 826, 909
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 2 cut(s) 23, 321
DpnI GATC 4 cut(s) 53, 169, 364, 964
DpnII GATC 4 cut(s) 51, 167, 362, 962
EaeI YGGCCR 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 712
EcoRII CCWGG 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 712
EcoT22I ATGCAT 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 712
FaeI CATG 3 cut(s) 440, 829, 912
FaqI GGGAC 1 cut(s) 513
FatI CATG 3 cut(s) 436, 825, 908
FauNDI CATATG 1 cut(s) 865
FbaI TGATCA 1 cut(s) 51
Fnu4HI GCNGC 3 cut(s) 90, 118, 420
FokI GGATG 7 cut(s) 194, 201, 396, 428, 478, 844, 952
Fsp4HI GCNGC 3 cut(s) 90, 118, 420
FspBI CTAG 2 cut(s) 75, 86
GlaI GCGC 1 cut(s) 544
GluI GCNGC 3 cut(s) 90, 118, 420
GsuI CTGGAG 2 cut(s) 61, 211
HaeII RGCGCY 1 cut(s) 546
HaeIII GGCC 2 cut(s) 680, 835
HapII CCGG 1 cut(s) 971
HhaI GCGC 1 cut(s) 545
Hin1II CATG 3 cut(s) 440, 829, 912
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HinfI GANTC 2 cut(s) 756, 974
HpaII CCGG 1 cut(s) 971
HphI GGTGA 2 cut(s) 422, 728
Hpy166II GTNNAC 1 cut(s) 327
Hpy188I TCNGA 2 cut(s) 552, 582
Hpy188III TCNNGA 6 cut(s) 49, 129, 290, 790, 848, 960
Hpy8I GTNNAC 1 cut(s) 327
HpyAV CCTTC 2 cut(s) 107, 126
HpyCH4III ACNGT 3 cut(s) 19, 163, 917
HpyCH4V TGCA 8 cut(s) 59, 120, 419, 485, 688, 739, 869, 901
HpyF3I CTNAG 2 cut(s) 23, 321
Hsp92II CATG 3 cut(s) 440, 829, 912
HspAI GCGC 1 cut(s) 543
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 4 cut(s) 51, 167, 362, 962
LmnI GCTCC 2 cut(s) 296, 469
Lsp1109I GCAGC 2 cut(s) 104, 431
LweI GCATC 3 cut(s) 374, 406, 888
MaeI CTAG 2 cut(s) 75, 86
MaeIII GTNAC 2 cut(s) 274, 340
MalI GATC 4 cut(s) 53, 169, 364, 964
MboI GATC 4 cut(s) 51, 167, 362, 962
MboII GAAGA 5 cut(s) 150, 153, 357, 452, 533
MflI RGATCY 2 cut(s) 167, 362
MhlI GDGCHC 1 cut(s) 606
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 4 cut(s) 206, 210, 280, 930
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 1 cut(s) 983
MmeI TCCRAC 3 cut(s) 491, 519, 871
MnlI CCTC 9 cut(s) 36, 172, 186, 406, 440, 541, 719, 871, 984
Mox20I TGGCCA 1 cut(s) 680
Mph1103I ATGCAT 1 cut(s) 903
MscI TGGCCA 1 cut(s) 680
MseI TTAA 2 cut(s) 209, 929
MslI CAYNNNNRTG 2 cut(s) 611, 874
Msp20I TGGCCA 1 cut(s) 680
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 1 cut(s) 971
MspR9I CCNGG 1 cut(s) 190
MvaI CCWGG 1 cut(s) 190
NdeI CATATG 1 cut(s) 865
NdeII GATC 4 cut(s) 51, 167, 362, 962
NlaIII CATG 3 cut(s) 440, 829, 912
NmuCI GTSAC 1 cut(s) 340
NsiI ATGCAT 1 cut(s) 903
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 754
OliI CACNNNNGTG 1 cut(s) 611
PciI ACATGT 1 cut(s) 825
PfeI GAWTC 1 cut(s) 756
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 3 cut(s) 91, 119, 421
PleI GAGTC 1 cut(s) 982
PpsI GAGTC 1 cut(s) 982
PscI ACATGT 1 cut(s) 825
PshBI ATTAAT 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PsuI RGATCY 2 cut(s) 167, 362
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
RseI CAYNNNNRTG 2 cut(s) 611, 874
SaqAI TTAA 2 cut(s) 209, 929
SatI GCNGC 3 cut(s) 90, 118, 420
Sau3AI GATC 4 cut(s) 51, 167, 362, 962
ScaI AGTACT 1 cut(s) 454
SchI GAGTC 1 cut(s) 983
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 1 cut(s) 606
SetI ASST 7 cut(s) 29, 47, 301, 424, 451, 737, 995
SfaNI GCATC 3 cut(s) 374, 406, 888
SfuI TTCGAA 1 cut(s) 754
SmiMI CAYNNNNRTG 2 cut(s) 611, 874
SmlI CTYRAG 1 cut(s) 958
SmoI CTYRAG 1 cut(s) 958
Sse9I AATT 4 cut(s) 206, 210, 280, 930
SsiI CCGC 2 cut(s) 90, 397
SspI AATATT 1 cut(s) 63
SspMI CTAG 2 cut(s) 75, 86
StyD4I CCNGG 1 cut(s) 188
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 19, 163, 917
TaqI TCGA 2 cut(s) 754, 849
TasI AATT 4 cut(s) 206, 210, 280, 930
TatI WGTACW 1 cut(s) 452
TauI GCSGC 1 cut(s) 92
TfiI GAWTC 1 cut(s) 756
Tru1I TTAA 2 cut(s) 209, 929
Tru9I TTAA 2 cut(s) 209, 929
TscAI CASTG 4 cut(s) 61, 329, 520, 613
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 117, 419
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 5 cut(s) 126, 198, 203, 453, 956
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 4 cut(s) 61, 329, 520, 613
Van91I CCANNNNNTGG 1 cut(s) 473
VspI ATTAAT 1 cut(s) 209
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 542
XspI CTAG 2 cut(s) 75, 86
ZrmI AGTACT 1 cut(s) 454
Zsp2I ATGCAT 1 cut(s) 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.