Rmu_co8453927.1_g000001

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8453927.1
OG0015883
Physical Location & Seq
Forward (+)
1 .. 1732
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8453927.1_g000001.1.cds

Sequence Viewer

Length: 1119 bp
accaaatactacaagcccgtccgcgacggcgtcatcaccaccgacggcgaccgcgccgtcgtcatcggcgaaaacggcctgtcgtaccagctccccggcgcgccgttcgagttccagtacagctactcggagacgccaaaggtcaagcctttggcgattcgcgagccggcgttcctgccgttcgcgccgccgacaatgccgcggccgtggaccggaaaagcgccgttgaagagcgccaaggagaagaagatgaagcggaaagtcccgctctttgactcgtttaacccgccggcgccgggaaccaaaggagtgaagcgggtcgaaatgcccggcccgtttgatttggggaagcgcccgaaacccggaaaaacgcgggaggagattctcggcgagccgttgaagcggtgggaggtgaaaatgctcgtaaaggagcagttgtccgatagccggcaagtcaatctcggaagagatggattgacgcataatatgttggatttgatacattcgcattggaagagacgcagggtgtgtagagtgaaatgtaaaggtgttccaactgtggatatggataacatttgccgccatattgaggaaagaactggggggaagatcattcatcgagttggtggtgtagtatatctttttcggggaagaaactataactataatacccgtccgcagtatccactgatgctgtggaaaccagctgctccagtttatcccaaactgatccaagaggctcctgaaggattgacaaaagatgaagctgaagagttgagaaagaaagggaaaagactcttgcctatatgtacattagccaaaaatggagtatacatctccttggttagagatgttaggcatgcttttgaaggaagttctttagtaaaggttgactgcaaagggatgcatgcaagtgattacaaaaagttgggtgctaagcttaaggaattggttccttgtgtactgctttcttttgatgatgaacagatattgatgtggaggggaccagattgggtttcaatgtcccaagaggtgcctccacattcagagtattctgatgttgatattacaacatcaaaagcgagaggtatgatcacaacttacattgcatatgcatga

Protein Analysis

372

Amino Acids

42.23

Weight (kDa)

9.65

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23400
fragaria_vesca FvH4_4g32880
malus_domestica MD13G1094700.v1.1
prunus_persica Prupe.1G250700_v2.0.a1
pyrus_communis pycom13g08250
rosa_chinensis RchiOBHm_Chr4g0441911
rosa_laevigata RLG00000006057
rosa_multiflora Rmu_co8453927.1_g000001
rosa_roxburghii Rroxscaffold_5G00382720
rosa_rugosa Rorug04G0336900
rosa_samantha Rh4AG388600 Rh4BG402300 Rh4CG417400 Rh4DG395600
rosa_wichuraiana Rw4G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 56
AccB1I GGYRCC 2 cut(s) 292, 1033
AccBSI CCGCTC 1 cut(s) 268
AccI GTMKAC 1 cut(s) 831
AccII CGCG 7 cut(s) 24, 54, 101, 162, 185, 202, 373
AclWI GGATC 1 cut(s) 724
AcoI YGGCCR 1 cut(s) 203
AcuI CTGAAG 2 cut(s) 765, 789
AcyI GRCGYC 3 cut(s) 30, 134, 293
AfaI GTAC 4 cut(s) 86, 119, 811, 963
AfiI CCNNNNNNNGG 5 cut(s) 212, 296, 362, 447, 589
AflII CTTAAG 1 cut(s) 941
AgsI TTSAA 4 cut(s) 229, 400, 869, 1020
AluBI AGCT 5 cut(s) 91, 123, 707, 767, 940
AluI AGCT 5 cut(s) 91, 123, 707, 767, 940
Alw26I GTCTC 2 cut(s) 125, 511
AlwI GGATC 1 cut(s) 724
AoxI GGCC 3 cut(s) 76, 203, 331
ApeKI GCWGC 1 cut(s) 707
AscI GGCGCGCC 1 cut(s) 99
Asp700I GAANNNNTTC 1 cut(s) 951
AspLEI GCGC 8 cut(s) 56, 101, 103, 187, 223, 236, 295, 354
AspS9I GGNCC 3 cut(s) 210, 332, 1004
AsuC2I CCSGG 4 cut(s) 96, 297, 330, 363
AsuHPI GGTGA 2 cut(s) 28, 424
AvaII GGWCC 2 cut(s) 210, 1004
BanI GGYRCC 2 cut(s) 292, 1033
BbvI GCAGC 1 cut(s) 694
BccI CCATC 1 cut(s) 464
BceAI ACGGC 9 cut(s) 41, 43, 61, 88, 91, 163, 190, 208, 379
BciVI GTATCC 1 cut(s) 693
BclI TGATCA 1 cut(s) 1092
BcnI CCSGG 4 cut(s) 96, 297, 330, 363
BcoDI GTCTC 2 cut(s) 125, 511
BfoI RGCGCY 4 cut(s) 224, 237, 296, 355
BfrI CTTAAG 1 cut(s) 941
BfuI GTATCC 1 cut(s) 693
BisI GCNGC 5 cut(s) 188, 200, 203, 580, 708
BlpI GCTNAGC 1 cut(s) 936
BlsI GCNGC 5 cut(s) 189, 201, 204, 581, 709
Bme1390I CCNGG 4 cut(s) 96, 297, 330, 363
Bme18I GGWCC 2 cut(s) 210, 1004
BmgT120I GGNCC 3 cut(s) 210, 332, 1004
BmiI GGNNCC 6 cut(s) 294, 301, 741, 954, 1005, 1035
BmrFI CCNGG 4 cut(s) 96, 297, 330, 363
BmrI ACTGGG 1 cut(s) 609
BmsI GCATC 2 cut(s) 681, 894
BmuI ACTGGG 1 cut(s) 609
BpmI CTGGAG 1 cut(s) 696
Bpu1102I GCTNAGC 1 cut(s) 936
BpuMI CCSGG 4 cut(s) 96, 297, 330, 363
BsaHI GRCGYC 3 cut(s) 30, 134, 293
BsaJI CCNNGG 5 cut(s) 94, 200, 206, 237, 840
BsaWI WCCGGW 1 cut(s) 212
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 5 cut(s) 212, 296, 362, 447, 589
Bse118I RCCGGY 3 cut(s) 166, 289, 447
Bse1I ACTGG 3 cut(s) 115, 604, 713
Bse3DI GCAATG 1 cut(s) 1104
BseDI CCNNGG 5 cut(s) 94, 200, 206, 237, 840
BseGI GGATG 1 cut(s) 909
BseLI CCNNNNNNNGG 5 cut(s) 212, 296, 362, 447, 589
BseMI GCAATG 1 cut(s) 1104
BseNI ACTGG 3 cut(s) 115, 604, 713
BsePI GCGCGC 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 392
BseX3I CGGCCG 1 cut(s) 203
BseXI GCAGC 1 cut(s) 694
Bsh1236I CGCG 7 cut(s) 24, 54, 101, 162, 185, 202, 373
Bsh1285I CGRYCG 2 cut(s) 52, 206
BshFI GGCC 3 cut(s) 78, 205, 333
BshNI GGYRCC 2 cut(s) 292, 1033
BsiEI CGRYCG 2 cut(s) 52, 206
BsiSI CCGG 8 cut(s) 96, 167, 213, 290, 296, 330, 363, 448
BslFI GGGAC 3 cut(s) 248, 1009, 1017
BslI CCNNNNNNNGG 5 cut(s) 212, 296, 362, 447, 589
BsmAI GTCTC 2 cut(s) 125, 511
BsmBI CGTCTC 2 cut(s) 125, 511
BsmFI GGGAC 3 cut(s) 248, 1009, 1017
BsnI GGCC 3 cut(s) 78, 205, 333
Bsp1407I TGTACA 1 cut(s) 809
Bsp143I GATC 3 cut(s) 609, 729, 1092
Bsp1720I GCTNAGC 1 cut(s) 936
Bsp68I TCGCGA 1 cut(s) 162
BspANI GGCC 3 cut(s) 78, 205, 333
BspFNI CGCG 7 cut(s) 24, 54, 101, 162, 185, 202, 373
BspLI GGNNCC 6 cut(s) 294, 301, 741, 954, 1005, 1035
BspPI GGATC 1 cut(s) 724
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 292, 1033
BspTI CTTAAG 1 cut(s) 941
BsrBI CCGCTC 1 cut(s) 268
BsrDI GCAATG 1 cut(s) 1104
BsrFI RCCGGY 3 cut(s) 166, 289, 447
BsrGI TGTACA 1 cut(s) 809
BsrI ACTGG 3 cut(s) 115, 604, 713
BssAI RCCGGY 3 cut(s) 166, 289, 447
BssECI CCNNGG 5 cut(s) 94, 200, 206, 237, 840
BssHII GCGCGC 1 cut(s) 99
BssMI GATC 3 cut(s) 609, 729, 1092
BssNAI GTATAC 1 cut(s) 832
BssNI GRCGYC 3 cut(s) 30, 134, 293
BssT1I CCWWGG 2 cut(s) 237, 840
Bst1107I GTATAC 1 cut(s) 832
Bst4CI ACNGT 1 cut(s) 559
Bst6I CTCTTC 4 cut(s) 224, 460, 509, 765
BstACI GRCGYC 3 cut(s) 30, 134, 293
BstAFI CTTAAG 1 cut(s) 941
BstAUI TGTACA 1 cut(s) 809
BstC8I GCNNGC 8 cut(s) 101, 164, 168, 291, 392, 449, 861, 909
BstDEI CTNAG 1 cut(s) 936
BstDSI CCRYGG 2 cut(s) 200, 206
BstF5I GGATG 1 cut(s) 909
BstFNI CGCG 7 cut(s) 24, 54, 101, 162, 185, 202, 373
BstH2I RGCGCY 4 cut(s) 224, 237, 296, 355
BstHHI GCGC 8 cut(s) 56, 101, 103, 187, 223, 236, 295, 354
BstKTI GATC 3 cut(s) 612, 732, 1095
BstMAI GTCTC 2 cut(s) 125, 511
BstMBI GATC 3 cut(s) 609, 729, 1092
BstMCI CGRYCG 2 cut(s) 52, 206
BstMWI GCNNNNNNNGC 4 cut(s) 75, 184, 196, 400
BstNSI RCATGY 2 cut(s) 863, 911
BstSCI CCNGG 4 cut(s) 94, 295, 328, 361
BstUI CGCG 7 cut(s) 24, 54, 101, 162, 185, 202, 373
BstV1I GCAGC 1 cut(s) 694
BstZ17I GTATAC 1 cut(s) 832
BstZI CGGCCG 1 cut(s) 203
BsuI GTATCC 1 cut(s) 693
BsuRI GGCC 3 cut(s) 78, 205, 333
BtgI CCRYGG 2 cut(s) 200, 206
BtsCI GGATG 1 cut(s) 909
BtsIMutI CAGTG 1 cut(s) 686
BtuMI TCGCGA 1 cut(s) 162
Cac8I GCNNGC 8 cut(s) 101, 164, 168, 291, 392, 449, 861, 909
CfoI GCGC 8 cut(s) 56, 101, 103, 187, 223, 236, 295, 354
Cfr10I RCCGGY 3 cut(s) 166, 289, 447
Cfr13I GGNCC 3 cut(s) 210, 332, 1004
Cfr42I CCGCGG 1 cut(s) 203
CseI GACGC 4 cut(s) 19, 142, 487, 528
Csp6I GTAC 4 cut(s) 85, 118, 810, 962
CviAII CATG 3 cut(s) 860, 908, 1116
CviQI GTAC 4 cut(s) 85, 118, 810, 962
DdeI CTNAG 1 cut(s) 936
DinI GGCGCC 1 cut(s) 294
DpnI GATC 3 cut(s) 611, 731, 1094
DpnII GATC 3 cut(s) 609, 729, 1092
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
EaeI YGGCCR 1 cut(s) 203
EagI CGGCCG 1 cut(s) 203
Eam1104I CTCTTC 4 cut(s) 224, 460, 509, 765
EarI CTCTTC 4 cut(s) 224, 460, 509, 765
EclXI CGGCCG 1 cut(s) 203
Eco130I CCWWGG 2 cut(s) 237, 840
Eco47I GGWCC 2 cut(s) 210, 1004
Eco52I CGGCCG 1 cut(s) 203
Eco57I CTGAAG 2 cut(s) 765, 789
EcoT14I CCWWGG 2 cut(s) 237, 840
EcoT22I ATGCAT 2 cut(s) 909, 1117
EgeI GGCGCC 1 cut(s) 294
EheI GGCGCC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 237, 840
Esp3I CGTCTC 2 cut(s) 125, 511
FaeI CATG 3 cut(s) 863, 911, 1119
FaqI GGGAC 3 cut(s) 248, 1009, 1017
FatI CATG 3 cut(s) 859, 907, 1115
FauI CCCGC 4 cut(s) 273, 294, 309, 366
FauNDI CATATG 1 cut(s) 1111
FbaI TGATCA 1 cut(s) 1092
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 5 cut(s) 188, 200, 203, 580, 708
FokI GGATG 1 cut(s) 916
Fsp4HI GCNGC 5 cut(s) 188, 200, 203, 580, 708
GlaI GCGC 8 cut(s) 55, 100, 102, 186, 222, 235, 294, 353
GluI GCNGC 5 cut(s) 188, 200, 203, 580, 708
GsuI CTGGAG 1 cut(s) 696
HaeII RGCGCY 4 cut(s) 224, 237, 296, 355
HaeIII GGCC 3 cut(s) 78, 205, 333
HapII CCGG 8 cut(s) 96, 167, 213, 290, 296, 330, 363, 448
HgaI GACGC 4 cut(s) 19, 142, 487, 528
HhaI GCGC 8 cut(s) 56, 101, 103, 187, 223, 236, 295, 354
Hin1I GRCGYC 3 cut(s) 30, 134, 293
Hin1II CATG 3 cut(s) 863, 911, 1119
Hin6I GCGC 8 cut(s) 54, 99, 101, 185, 221, 234, 293, 352
HinP1I GCGC 8 cut(s) 54, 99, 101, 185, 221, 234, 293, 352
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 938
HinfI GANTC 4 cut(s) 157, 275, 382, 795
HpaII CCGG 8 cut(s) 96, 167, 213, 290, 296, 330, 363, 448
HphI GGTGA 2 cut(s) 28, 424
Hpy166II GTNNAC 4 cut(s) 210, 832, 892, 962
Hpy188I TCNGA 5 cut(s) 130, 442, 464, 1048, 1057
Hpy188III TCNNGA 2 cut(s) 161, 743
Hpy8I GTNNAC 4 cut(s) 210, 832, 892, 962
Hpy99I CGWCG 3 cut(s) 29, 47, 62
HpyAV CCTTC 2 cut(s) 740, 863
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4V TGCA 5 cut(s) 897, 907, 911, 1109, 1115
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 184, 196, 400
HpyF3I CTNAG 1 cut(s) 936
Hsp92I GRCGYC 3 cut(s) 30, 134, 293
Hsp92II CATG 3 cut(s) 863, 911, 1119
HspAI GCGC 8 cut(s) 54, 99, 101, 185, 221, 234, 293, 352
KasI GGCGCC 1 cut(s) 292
KroI GCCGGC 3 cut(s) 166, 289, 447
KroNI GCCGGC 3 cut(s) 168, 291, 449
Ksp22I TGATCA 1 cut(s) 1092
KspI CCGCGG 1 cut(s) 203
Kzo9I GATC 3 cut(s) 609, 729, 1092
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 4 cut(s) 96, 430, 715, 745
Lsp1109I GCAGC 1 cut(s) 694
LweI GCATC 2 cut(s) 681, 894
MalI GATC 3 cut(s) 611, 731, 1094
MbiI CCGCTC 1 cut(s) 268
MboI GATC 3 cut(s) 609, 729, 1092
MboII GAAGA 8 cut(s) 241, 256, 259, 477, 526, 619, 663, 782
MluCI AATT 1 cut(s) 947
Mly113I GGCGCC 1 cut(s) 293
MlyI GAGTC 2 cut(s) 269, 789
MmeI TCCRAC 2 cut(s) 471, 578
MnlI CCTC 8 cut(s) 370, 403, 583, 730, 993, 1024, 1047, 1079
Mph1103I ATGCAT 2 cut(s) 909, 1117
MreI CGCCGGCG 1 cut(s) 289
MroNI GCCGGC 3 cut(s) 166, 289, 447
MroXI GAANNNNTTC 1 cut(s) 951
MseI TTAA 2 cut(s) 282, 942
MslI CAYNNNNRTG 2 cut(s) 912, 1114
MspA1I CMGCKG 2 cut(s) 202, 707
MspCI CTTAAG 1 cut(s) 941
MspI CCGG 8 cut(s) 96, 167, 213, 290, 296, 330, 363, 448
MspR9I CCNGG 4 cut(s) 96, 297, 330, 363
MvnI CGCG 7 cut(s) 24, 54, 101, 162, 185, 202, 373
MwoI GCNNNNNNNGC 4 cut(s) 75, 184, 196, 400
NaeI GCCGGC 3 cut(s) 168, 291, 449
NarI GGCGCC 1 cut(s) 293
NciI CCSGG 4 cut(s) 96, 297, 330, 363
NdeI CATATG 1 cut(s) 1111
NdeII GATC 3 cut(s) 609, 729, 1092
NgoMIV GCCGGC 3 cut(s) 166, 289, 447
NlaIII CATG 3 cut(s) 863, 911, 1119
NlaIV GGNNCC 6 cut(s) 294, 301, 741, 954, 1005, 1035
NmeAIII GCCGAG 1 cut(s) 366
NruI TCGCGA 1 cut(s) 162
NsiI ATGCAT 2 cut(s) 909, 1117
NspI RCATGY 2 cut(s) 863, 911
PaeI GCATGC 2 cut(s) 863, 911
PalAI GGCGCGCC 1 cut(s) 99
PauI GCGCGC 1 cut(s) 99
PciSI GCTCTTC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PdiI GCCGGC 3 cut(s) 168, 291, 449
PdmI GAANNNNTTC 1 cut(s) 951
PfeI GAWTC 2 cut(s) 157, 382
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 5 cut(s) 189, 201, 204, 581, 709
PleI GAGTC 2 cut(s) 269, 789
PluTI GGCGCC 1 cut(s) 296
PpsI GAGTC 2 cut(s) 269, 789
PspN4I GGNNCC 6 cut(s) 294, 301, 741, 954, 1005, 1035
PspPI GGNCC 3 cut(s) 210, 332, 1004
PsyI GACNNNGTC 1 cut(s) 29
PteI GCGCGC 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 707
RruI TCGCGA 1 cut(s) 162
RsaI GTAC 4 cut(s) 86, 119, 811, 963
RsaNI GTAC 4 cut(s) 85, 118, 810, 962
RseI CAYNNNNRTG 2 cut(s) 912, 1114
SacII CCGCGG 1 cut(s) 203
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 282, 942
SatI GCNGC 5 cut(s) 188, 200, 203, 580, 708
Sau3AI GATC 3 cut(s) 609, 729, 1092
Sau96I GGNCC 3 cut(s) 210, 332, 1004
SchI GAGTC 2 cut(s) 269, 789
ScrFI CCNGG 4 cut(s) 96, 297, 330, 363
SfaNI GCATC 2 cut(s) 681, 894
SfoI GGCGCC 1 cut(s) 294
Sfr303I CCGCGG 1 cut(s) 203
SgrAI CRCCGGYG 1 cut(s) 289
SgrBI CCGCGG 1 cut(s) 203
SgsI GGCGCGCC 1 cut(s) 99
SinI GGWCC 2 cut(s) 210, 1004
SmiMI CAYNNNNRTG 2 cut(s) 912, 1114
SmlI CTYRAG 1 cut(s) 941
SmoI CTYRAG 1 cut(s) 941
SphI GCATGC 2 cut(s) 863, 911
Sse9I AATT 1 cut(s) 947
SspDI GGCGCC 1 cut(s) 292
StyD4I CCNGG 4 cut(s) 94, 295, 328, 361
StyI CCWWGG 2 cut(s) 237, 840
TaaI ACNGT 1 cut(s) 559
TaqI TCGA 3 cut(s) 108, 321, 619
TasI AATT 1 cut(s) 947
TatI WGTACW 3 cut(s) 117, 809, 961
TauI GCSGC 4 cut(s) 190, 202, 205, 582
TfiI GAWTC 2 cut(s) 157, 382
Tru1I TTAA 2 cut(s) 282, 942
Tru9I TTAA 2 cut(s) 282, 942
TscAI CASTG 1 cut(s) 693
TseI GCWGC 1 cut(s) 707
TspDTI ATGAA 4 cut(s) 266, 605, 777, 996
TspRI CASTG 1 cut(s) 693
Tth111I GACNNNGTC 1 cut(s) 29
Vha464I CTTAAG 1 cut(s) 941
VpaK11BI GGWCC 2 cut(s) 210, 1004
XceI RCATGY 2 cut(s) 863, 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 693
XmiI GTMKAC 1 cut(s) 831
XmnI GAANNNNTTC 1 cut(s) 951
Zsp2I ATGCAT 2 cut(s) 909, 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.