Rroxscaffold_5G00382720

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015883
Physical Location & Seq
Reverse (-)
62545163 .. 62548445
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00382720.1

Sequence Viewer

Length: 792 bp
ATGCTGTGGAAACCAGCCGCTCCGGTTTATCCCAAACTGATCCAAGAGGCTCCTGAAGGATTGACAAAAGATGAAGCTGAAGAGTTGAGAAAGAAAGGGAAAAGGCTCTTGCCTATATGTACATTAGCCAAAAATGGAGTATACATCTCCTTGGTTAGAGATGTTAGGCATGCTTTTGAAGGAAGTTCTTTAGTAAAGGTTGACTGCAAAGGGATGCATGCAAGTGATTACAAAAAGTTGGGTGCTAAGCTTAAGGAATTGGTTCCTTGTGTACTGCTTTCTTTTGATGATGAACAGATATTGATGTGGAGGGGACCAGATTGGGTTTCAATGTCCCAAGAGGTGCCTCCGCATTCAGAGTATTCTGATGTTGATAGTACAACATCAAAAGCGAGAGTTAAGGATGACGATGATTCTAGTAAGCCAGACACGAAAACAGTGATATCAAGCCCTAAAATGATGTCGCTCTGGAAACACTCAATTGAAACAAACAAAGCATTGTTGCTTGATGAACTTGACCTTGATCCCGATGCTCTCTTGAAGAAGGTTGAGGAATTCGAAGGCATTTCACAGGCCACAGAGCACTCCTATCCAGCATTAATCCTCTCAAGCGAAGATGGCACCAGCAGCGCAATCCAAGTGTATGAAGATGTTCCTCAGAATGGCCCTTATAGCGAATACGAAGACTCTTACAGTGATAACAATGACGATTATGTATACGATGATGATACCGATGACGATGCGTTATACGATAGTGATTCACCCCCTTGGGGTCATTACCCGTTGATGTAA

Protein Analysis

263

Amino Acids

29.57

Weight (kDa)

4.49

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 21 - 104 4.5e-15 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015883)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23400
fragaria_vesca FvH4_4g32880
malus_domestica MD13G1094700.v1.1
prunus_persica Prupe.1G250700_v2.0.a1
pyrus_communis pycom13g08250
rosa_chinensis RchiOBHm_Chr4g0441911
rosa_laevigata RLG00000006057
rosa_multiflora Rmu_co8453927.1_g000001
rosa_roxburghii Rroxscaffold_5G00382720
rosa_rugosa Rorug04G0336900
rosa_samantha Rh4AG388600 Rh4BG402300 Rh4CG417400 Rh4DG395600
rosa_wichuraiana Rw4G033560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 343, 620
AccBSI CCGCTC 1 cut(s) 20
AccI GTMKAC 2 cut(s) 141, 717
AciI CCGC 2 cut(s) 18, 350
AclWI GGATC 2 cut(s) 34, 518
AcsI RAATTY 1 cut(s) 554
AcuI CTGAAG 2 cut(s) 75, 99
AfaI GTAC 3 cut(s) 121, 273, 379
AfiI CCNNNNNNNGG 2 cut(s) 662, 770
AflII CTTAAG 1 cut(s) 251
AgsI TTSAA 4 cut(s) 179, 330, 485, 541
AluBI AGCT 2 cut(s) 77, 250
AluI AGCT 2 cut(s) 77, 250
Alw21I GWGCWC 1 cut(s) 585
AlwI GGATC 2 cut(s) 34, 518
AoxI GGCC 2 cut(s) 573, 664
ApeKI GCWGC 1 cut(s) 627
ApoI RAATTY 1 cut(s) 554
AseI ATTAAT 1 cut(s) 599
Asp700I GAANNNNTTC 2 cut(s) 261, 651
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 2 cut(s) 314, 665
AsuHPI GGTGA 1 cut(s) 753
AsuII TTCGAA 1 cut(s) 558
AvaII GGWCC 1 cut(s) 314
BanI GGYRCC 2 cut(s) 343, 620
BbsI GAAGAC 1 cut(s) 690
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 639
BccI CCATC 1 cut(s) 611
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BfaI CTAG 1 cut(s) 417
BfrI CTTAAG 1 cut(s) 251
BisI GCNGC 2 cut(s) 18, 628
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 2 cut(s) 19, 629
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 314, 665
BmiI GGNNCC 5 cut(s) 51, 264, 315, 345, 622
BmsI GCATC 3 cut(s) 204, 520, 730
BpiI GAAGAC 1 cut(s) 690
Bpu1102I GCTNAGC 1 cut(s) 246
Bpu14I TTCGAA 1 cut(s) 558
BpuEI CTTGAG 1 cut(s) 592
BsaJI CCNNGG 2 cut(s) 150, 767
BsaWI WCCGGW 1 cut(s) 22
Bsc4I CCNNNNNNNGG 2 cut(s) 662, 770
BseDI CCNNGG 2 cut(s) 150, 767
BseGI GGATG 2 cut(s) 219, 409
BseLI CCNNNNNNNGG 2 cut(s) 662, 770
BseMII CTCAG 1 cut(s) 671
BseXI GCAGC 1 cut(s) 639
BshFI GGCC 2 cut(s) 575, 666
BshNI GGYRCC 2 cut(s) 343, 620
BsiHKAI GWGCWC 1 cut(s) 585
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 2 cut(s) 319, 327
BslI CCNNNNNNNGG 2 cut(s) 662, 770
BsmFI GGGAC 2 cut(s) 319, 327
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 2 cut(s) 575, 666
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 585
Bsp1407I TGTACA 1 cut(s) 119
Bsp143I GATC 2 cut(s) 39, 523
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 2 cut(s) 18, 350
BspANI GGCC 2 cut(s) 575, 666
BspCNI CTCAG 1 cut(s) 670
BspLI GGNNCC 5 cut(s) 51, 264, 315, 345, 622
BspPI GGATC 2 cut(s) 34, 518
BspT104I TTCGAA 1 cut(s) 558
BspT107I GGYRCC 2 cut(s) 343, 620
BspTI CTTAAG 1 cut(s) 251
BsrBI CCGCTC 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 150, 767
BssMI GATC 2 cut(s) 39, 523
BssNAI GTATAC 2 cut(s) 142, 718
BssT1I CCWWGG 2 cut(s) 150, 767
Bst1107I GTATAC 2 cut(s) 142, 718
Bst4CI ACNGT 2 cut(s) 439, 695
Bst6I CTCTTC 1 cut(s) 75
BstAFI CTTAAG 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 119
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 2 cut(s) 171, 219
BstDEI CTNAG 2 cut(s) 246, 657
BstF5I GGATG 2 cut(s) 219, 409
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 2 cut(s) 42, 526
BstMBI GATC 2 cut(s) 39, 523
BstMWI GCNNNNNNNGC 3 cut(s) 618, 627, 672
BstNSI RCATGY 2 cut(s) 173, 221
BstV1I GCAGC 1 cut(s) 639
BstV2I GAAGAC 1 cut(s) 690
BstZ17I GTATAC 2 cut(s) 142, 718
BsuRI GGCC 2 cut(s) 575, 666
BtsCI GGATG 2 cut(s) 219, 409
BtsIMutI CAGTG 2 cut(s) 444, 700
Cac8I GCNNGC 2 cut(s) 171, 219
CfoI GCGC 1 cut(s) 632
Cfr13I GGNCC 2 cut(s) 314, 665
Csp6I GTAC 3 cut(s) 120, 272, 378
CviAII CATG 2 cut(s) 170, 218
CviQI GTAC 3 cut(s) 120, 272, 378
DdeI CTNAG 2 cut(s) 246, 657
DpnI GATC 2 cut(s) 41, 525
DpnII GATC 2 cut(s) 39, 523
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Eco130I CCWWGG 2 cut(s) 150, 767
Eco32I GATATC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 2 cut(s) 75, 99
EcoRI GAATTC 1 cut(s) 554
EcoRV GATATC 1 cut(s) 444
EcoT14I CCWWGG 2 cut(s) 150, 767
EcoT22I ATGCAT 1 cut(s) 219
ErhI CCWWGG 2 cut(s) 150, 767
FaeI CATG 2 cut(s) 173, 221
FaqI GGGAC 2 cut(s) 319, 327
FatI CATG 2 cut(s) 169, 217
FblI GTMKAC 2 cut(s) 141, 717
Fnu4HI GCNGC 2 cut(s) 18, 628
FokI GGATG 2 cut(s) 226, 416
Fsp4HI GCNGC 2 cut(s) 18, 628
FspBI CTAG 1 cut(s) 417
GlaI GCGC 1 cut(s) 631
GluI GCNGC 2 cut(s) 18, 628
HaeIII GGCC 2 cut(s) 575, 666
HapII CCGG 1 cut(s) 23
HhaI GCGC 1 cut(s) 632
Hin1II CATG 2 cut(s) 173, 221
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 248
HinfI GANTC 3 cut(s) 413, 686, 758
HpaII CCGG 1 cut(s) 23
HphI GGTGA 1 cut(s) 753
Hpy166II GTNNAC 4 cut(s) 142, 202, 272, 718
Hpy188I TCNGA 3 cut(s) 358, 367, 660
Hpy188III TCNNGA 4 cut(s) 53, 469, 527, 538
Hpy8I GTNNAC 4 cut(s) 142, 202, 272, 718
HpyAV CCTTC 4 cut(s) 50, 173, 538, 554
HpyCH4III ACNGT 2 cut(s) 439, 695
HpyCH4V TGCA 3 cut(s) 207, 217, 221
HpyF10VI GCNNNNNNNGC 3 cut(s) 618, 627, 672
HpyF3I CTNAG 2 cut(s) 246, 657
Hsp92II CATG 2 cut(s) 173, 221
HspAI GCGC 1 cut(s) 630
Kzo9I GATC 2 cut(s) 39, 523
LmnI GCTCC 2 cut(s) 25, 55
LpnPI CCDG 9 cut(s) 27, 36, 66, 330, 438, 454, 557, 606, 637
Lsp1109I GCAGC 1 cut(s) 639
LweI GCATC 3 cut(s) 204, 520, 730
MaeI CTAG 1 cut(s) 417
MalI GATC 2 cut(s) 41, 525
MbiI CCGCTC 1 cut(s) 20
MboI GATC 2 cut(s) 39, 523
MboII GAAGA 5 cut(s) 92, 553, 626, 659, 695
MfeI CAATTG 1 cut(s) 480
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 3 cut(s) 257, 480, 554
MlyI GAGTC 1 cut(s) 680
MnlI CCTC 7 cut(s) 40, 303, 334, 357, 544, 614, 666
Mph1103I ATGCAT 1 cut(s) 219
MroXI GAANNNNTTC 2 cut(s) 261, 651
MseI TTAA 3 cut(s) 252, 399, 599
MslI CAYNNNNRTG 1 cut(s) 222
MspCI CTTAAG 1 cut(s) 251
MspI CCGG 1 cut(s) 23
MunI CAATTG 1 cut(s) 480
Mva1269I GAATGC 1 cut(s) 352
MwoI GCNNNNNNNGC 3 cut(s) 618, 627, 672
NdeII GATC 2 cut(s) 39, 523
NlaIII CATG 2 cut(s) 173, 221
NlaIV GGNNCC 5 cut(s) 51, 264, 315, 345, 622
NsiI ATGCAT 1 cut(s) 219
NspI RCATGY 2 cut(s) 173, 221
NspV TTCGAA 1 cut(s) 558
PaeI GCATGC 2 cut(s) 173, 221
PctI GAATGC 1 cut(s) 352
PdmI GAANNNNTTC 2 cut(s) 261, 651
PfeI GAWTC 2 cut(s) 413, 758
PkrI GCNGC 2 cut(s) 19, 629
PleI GAGTC 1 cut(s) 680
PpsI GAGTC 1 cut(s) 680
PshBI ATTAAT 1 cut(s) 599
PspN4I GGNNCC 5 cut(s) 51, 264, 315, 345, 622
PspPI GGNCC 2 cut(s) 314, 665
RsaI GTAC 3 cut(s) 121, 273, 379
RsaNI GTAC 3 cut(s) 120, 272, 378
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 3 cut(s) 252, 399, 599
SatI GCNGC 2 cut(s) 18, 628
Sau3AI GATC 2 cut(s) 39, 523
Sau96I GGNCC 2 cut(s) 314, 665
SchI GAGTC 1 cut(s) 680
SduI GDGCHC 1 cut(s) 585
SetI ASST 6 cut(s) 79, 201, 252, 345, 522, 549
SfaNI GCATC 3 cut(s) 204, 520, 730
SfuI TTCGAA 1 cut(s) 558
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 2 cut(s) 251, 607
SmoI CTYRAG 2 cut(s) 251, 607
SphI GCATGC 2 cut(s) 173, 221
Sse9I AATT 3 cut(s) 257, 480, 554
SsiI CCGC 2 cut(s) 18, 350
SspMI CTAG 1 cut(s) 417
StyI CCWWGG 2 cut(s) 150, 767
TaaI ACNGT 2 cut(s) 439, 695
TaqI TCGA 1 cut(s) 558
TasI AATT 3 cut(s) 257, 480, 554
TatI WGTACW 3 cut(s) 119, 271, 377
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 2 cut(s) 413, 758
Tru1I TTAA 3 cut(s) 252, 399, 599
Tru9I TTAA 3 cut(s) 252, 399, 599
TscAI CASTG 2 cut(s) 444, 700
TseI GCWGC 1 cut(s) 627
TspDTI ATGAA 4 cut(s) 87, 306, 525, 660
TspRI CASTG 2 cut(s) 444, 700
Vha464I CTTAAG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 314
VspI ATTAAT 1 cut(s) 599
XapI RAATTY 1 cut(s) 554
XceI RCATGY 2 cut(s) 173, 221
XmiI GTMKAC 2 cut(s) 141, 717
XmnI GAANNNNTTC 2 cut(s) 261, 651
XspI CTAG 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.