Prupe.1G559300_v2.0.a1

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0017200
Physical Location & Seq
Forward (+)
45659721 .. 45662830
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G559300.1

Sequence Viewer

Length: 1707 bp
ATGGCTAATGGCATAGTGCATTTTGCTTTCTCTGTTCTTCAACTTTTTCTTATTTTTGATGCCGCAAATGGGAAAACCCATCGCCATAACTTTGTGGTGAAGTCATCTTCTTATACTCGACTATGTAGCACTAAAGACATCTTGACTGTGAATGGGCAATTTCCAGGGCCAAGTTTGAAAGCTCATAGAGGAGATAAAATGATCATTAAGGTCTATAACAAGGCAAACTACAATATCACTTTTCATTGGCATGGAATTAAGCAGCCAAGAAATCCCTGGTCAGATGGACCTGAGTATATTACACAGTGCCCGATTAGGCCAGGAAACAAGTACACTTACAAAATTGAGTTTACAACAGAAGAAGGAACAATGTGGTGGCATGCACACAGTGGATGGGCAAGAGCAACAGTTCATGGTGCCATTTTGGTCTATCCAAAGCCTGGTTCTCACTATCCCTTCTCCAAACCCTATGCAGAGGTTCCAATCATACTAGGTGAATGGTGGAAGAAAAATGTAATGGAGATACCAAGAAATGCAAATATAACAGGAGGAGAGCCTATATTGTCAGATGCATATACCATAAATGGGAAACCAGGCCATCTCTATCCATGCTCCAAATCAGGGATTTTCGAAATGACAGTGGAGCATGGAAAAACCTATCTGCTTCGGATAATTAGCGCAGTAATGGATGAAGAGCTTTTCTTTGGCATAGCAAACCACAAAATGATATTGGTAGGAAGAGACGGATCCTACACAAAACAAGTTGTGACCTCTTACATTATGATAGCTCCTGGCCAATCCATGGATGTTTTATTACAAGCAAACCAACCCCCAAGCTTCTATTTCATGGCTGCAAGAGCATATTCAAGTGCCATTGGAGCTGGTTTTGACAAAACCATGACAACGGCCATTCTCAAATACCAAGCTTCATACCATGAACCTCCCGAATTCCCTCATTATAAATTTCCACATCTGCCCCCTTATGATAGAACACAAGCATCAACTGACTTCACCAAGAGAATCAGGAGCCTTGCAACAAAAGATCACCCAACCAACGTCCCATTACATGTAAACACCCACCTCTTCTTCACCATTTCAGTAAACCTCCTTAATTGTAGTAACAAACCATGCACTGGTCCTTTTGGTAAAAGATTTGCTGCCAGTGTGAACAACATAAGCTTTGTCGCCCCATCCATCGACATACTTCGAGCTTACTACTATAAGATCCCGGGAGTTTTCGAGAATGATTTTCCCAAAAAACCCCCAATGGAATTTAATTATACCGGTGAAGATCTTCCTGAGAACCTGTTGACACCTTCCTACGGCACGAAGGTGGTGGTTTTGGAGTACAATGCGAGTGTTGAGCTGGTTTTGCAAGGAACTAATGTCCTAGCAAGTGATAACCATCCTGTGCATTTGCATGGATATAACTTTTATGTTGTTGGCTGGGGCTTTGGAAACTTCAATCCCAAGAAAGACCCTTTGAATTATAACCTTGTTGATCCCCCAGAGGAGAATACAGTTGGAGTCCATAAGAATGGTTGGGTTGCAATCAGATTCAGAGCTGATAATCCAGGTGTGTGGTTGATGCATTGTCACATAGAGCGCCACCAAACATGGGGAATGACTGTGGTATTCCTAGTCAAAAATGGTAATAGTCCTCAAAATAAAATTCTACCACCACCTCACGATTTGCCTACTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

569

Amino Acids

64.12

Weight (kDa)

8.98

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09360
fragaria_vesca FvH4_5g30720
malus_domestica MD15G1412600.v1.1
prunus_persica Prupe.1G559300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0225621
rosa_laevigata RLG00000001800
rosa_multiflora Rmu_co8516127.1_g000001
rosa_roxburghii Rroxscaffold_3G00233630
rosa_rugosa Rorug07G0231900
rosa_samantha Rh7AG371900 Rh7BG365800 Rh7DG376600
rosa_wichuraiana Rw7G031780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 960, 1491
AccB1I GGYRCC 1 cut(s) 416
AccB7I CCANNNNNTGG 3 cut(s) 440, 802, 1133
AciI CCGC 1 cut(s) 63
AclWI GGATC 4 cut(s) 741, 754, 1219, 1496
AcoI YGGCCR 2 cut(s) 793, 906
AcsI RAATTY 4 cut(s) 947, 962, 1271, 1671
AdeI CACNNNGTG 1 cut(s) 389
AfaI GTAC 2 cut(s) 332, 1349
AfiI CCNNNNNNNGG 8 cut(s) 69, 440, 585, 621, 802, 1133, 1322, 1618
AflIII ACRYGT 1 cut(s) 1066
AgeI ACCGGT 1 cut(s) 1283
AgsI TTSAA 5 cut(s) 41, 178, 867, 1465, 1486
AjnI CCWGG 7 cut(s) 163, 275, 319, 439, 592, 790, 1573
AjuI GAANNNNNNNTTGG 2 cut(s) 427, 459
AleI CACNNNNGTG 1 cut(s) 1331
Alw26I GTCTC 1 cut(s) 735
AlwI GGATC 4 cut(s) 741, 754, 1219, 1496
Ama87I CYCGRG 1 cut(s) 1228
AoxI GGCC 5 cut(s) 167, 317, 595, 793, 906
ApeKI GCWGC 3 cut(s) 262, 851, 1157
ApoI RAATTY 4 cut(s) 947, 962, 1271, 1671
AsiGI ACCGGT 1 cut(s) 1283
Asp700I GAANNNNTTC 1 cut(s) 1293
AspLEI GCGC 2 cut(s) 680, 1608
AspS9I GGNCC 3 cut(s) 167, 287, 1136
AsuC2I CCSGG 2 cut(s) 1229, 1230
AsuHPI GGTGA 6 cut(s) 109, 506, 1003, 1037, 1081, 1298
AsuII TTCGAA 1 cut(s) 630
AvaI CYCGRG 1 cut(s) 1228
AvaII GGWCC 2 cut(s) 287, 1136
BaeGI GKGCMC 1 cut(s) 311
BalI TGGCCA 1 cut(s) 795
BamHI GGATCC 1 cut(s) 746
BanI GGYRCC 1 cut(s) 416
BbvI GCAGC 3 cut(s) 274, 838, 1144
BccI CCATC 7 cut(s) 87, 278, 387, 606, 1198, 1202, 1413
BceAI ACGGC 2 cut(s) 921, 1339
BciT130I CCWGG 7 cut(s) 165, 277, 321, 441, 594, 792, 1575
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 2 cut(s) 1229, 1230
BcoDI GTCTC 1 cut(s) 735
BfaI CTAG 3 cut(s) 491, 1391, 1640
BfoI RGCGCY 1 cut(s) 1609
BglII AGATCT 1 cut(s) 1291
BisI GCNGC 4 cut(s) 63, 263, 852, 1158
BlsI GCNGC 4 cut(s) 64, 264, 853, 1159
Bme1390I CCNGG 9 cut(s) 165, 277, 321, 441, 594, 792, 1229, 1230, 1575
Bme18I GGWCC 2 cut(s) 287, 1136
BmeT110I CYCGRG 1 cut(s) 1228
BmgT120I GGNCC 3 cut(s) 167, 287, 1136
BmiI GGNNCC 4 cut(s) 418, 480, 748, 1028
BmrFI CCNGG 9 cut(s) 165, 277, 321, 441, 594, 792, 1229, 1230, 1575
BmsI GCATC 4 cut(s) 49, 559, 1007, 1578
Bpu14I TTCGAA 1 cut(s) 630
BpuMI CCSGG 2 cut(s) 1229, 1230
BsaBI GATNNNNATC 1 cut(s) 1404
BsaJI CCNNGG 4 cut(s) 164, 275, 801, 1228
BsaWI WCCGGW 1 cut(s) 1283
Bsc4I CCNNNNNNNGG 8 cut(s) 69, 440, 585, 621, 802, 1133, 1322, 1618
Bse118I RCCGGY 1 cut(s) 1283
Bse1I ACTGG 2 cut(s) 1138, 1161
Bse8I GATNNNNATC 1 cut(s) 1404
BseBI CCWGG 7 cut(s) 165, 277, 321, 441, 594, 792, 1575
BseDI CCNNGG 4 cut(s) 164, 275, 801, 1228
BseGI GGATG 5 cut(s) 398, 694, 811, 1190, 1405
BseJI GATNNNNATC 1 cut(s) 1404
BseLI CCNNNNNNNGG 8 cut(s) 69, 440, 585, 621, 802, 1133, 1322, 1618
BseMII CTCAG 2 cut(s) 282, 1290
BseNI ACTGG 2 cut(s) 1138, 1161
BseRI GAGGAG 3 cut(s) 204, 564, 1526
BseSI GKGCMC 1 cut(s) 311
BseXI GCAGC 3 cut(s) 274, 838, 1144
BseYI CCCAGC 1 cut(s) 1446
BshFI GGCC 5 cut(s) 169, 319, 597, 795, 908
BshNI GGYRCC 1 cut(s) 416
BshTI ACCGGT 1 cut(s) 1283
BsiHKCI CYCGRG 1 cut(s) 1228
BsiSI CCGG 2 cut(s) 1229, 1284
BslFI GGGAC 1 cut(s) 1043
BslI CCNNNNNNNGG 8 cut(s) 69, 440, 585, 621, 802, 1133, 1322, 1618
BsmAI GTCTC 1 cut(s) 735
BsmBI CGTCTC 1 cut(s) 735
BsmFI GGGAC 1 cut(s) 1043
BsnI GGCC 5 cut(s) 169, 319, 597, 795, 908
BsoBI CYCGRG 1 cut(s) 1228
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 1 cut(s) 311
Bsp143I GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
Bsp19I CCATGG 1 cut(s) 801
BspACI CCGC 1 cut(s) 63
BspANI GGCC 5 cut(s) 169, 319, 597, 795, 908
BspCNI CTCAG 2 cut(s) 283, 1291
BspLI GGNNCC 4 cut(s) 418, 480, 748, 1028
BspPI GGATC 4 cut(s) 741, 754, 1219, 1496
BspQI GCTCTTC 1 cut(s) 687
BspT104I TTCGAA 1 cut(s) 630
BspT107I GGYRCC 1 cut(s) 416
BsrFI RCCGGY 1 cut(s) 1283
BsrI ACTGG 2 cut(s) 1138, 1161
BssAI RCCGGY 1 cut(s) 1283
BssECI CCNNGG 4 cut(s) 164, 275, 801, 1228
BssMI GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
BssT1I CCWWGG 1 cut(s) 801
Bst2UI CCWGG 7 cut(s) 165, 277, 321, 441, 594, 792, 1575
Bst4CI ACNGT 7 cut(s) 148, 306, 389, 409, 640, 1522, 1630
Bst6I CTCTTC 3 cut(s) 687, 733, 1088
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 2 cut(s) 291, 1299
BstDSI CCRYGG 1 cut(s) 801
BstF5I GGATG 5 cut(s) 398, 694, 811, 1190, 1405
BstH2I RGCGCY 1 cut(s) 1609
BstHHI GCGC 2 cut(s) 680, 1608
BstKTI GATC 6 cut(s) 204, 749, 1045, 1227, 1294, 1504
BstMAI GTCTC 1 cut(s) 735
BstMBI GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
BstMWI GCNNNNNNNGC 3 cut(s) 857, 878, 1372
BstNI CCWGG 7 cut(s) 165, 277, 321, 441, 594, 792, 1575
BstNSI RCATGY 2 cut(s) 383, 1070
BstSCI CCNGG 9 cut(s) 163, 275, 319, 439, 592, 790, 1227, 1228, 1573
BstSLI GKGCMC 1 cut(s) 311
BstV1I GCAGC 3 cut(s) 274, 838, 1144
BstX2I RGATCY 3 cut(s) 746, 1224, 1291
BstXI CCANNNNNNTGG 2 cut(s) 1538, 1581
BstYI RGATCY 3 cut(s) 746, 1224, 1291
BsuRI GGCC 5 cut(s) 169, 319, 597, 795, 908
BtgI CCRYGG 1 cut(s) 801
BtgZI GCGATG 1 cut(s) 65
BtsCI GGATG 5 cut(s) 398, 694, 811, 1190, 1405
BtsIMutI CAGTG 5 cut(s) 311, 394, 645, 1131, 1168
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 2 cut(s) 680, 1608
Cfr10I RCCGGY 1 cut(s) 1283
Cfr13I GGNCC 3 cut(s) 167, 287, 1136
Cfr9I CCCGGG 1 cut(s) 1228
Csp6I GTAC 2 cut(s) 331, 1348
CspAI ACCGGT 1 cut(s) 1283
CviQI GTAC 2 cut(s) 331, 1348
DdeI CTNAG 2 cut(s) 291, 1299
DpnI GATC 6 cut(s) 203, 748, 1044, 1226, 1293, 1503
DpnII GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
DraIII CACNNNGTG 1 cut(s) 389
EaeI YGGCCR 2 cut(s) 793, 906
Eam1104I CTCTTC 3 cut(s) 687, 733, 1088
EarI CTCTTC 3 cut(s) 687, 733, 1088
Eco130I CCWWGG 1 cut(s) 801
Eco47I GGWCC 2 cut(s) 287, 1136
Eco88I CYCGRG 1 cut(s) 1228
EcoRI GAATTC 1 cut(s) 947
EcoRII CCWGG 7 cut(s) 163, 275, 319, 439, 592, 790, 1573
EcoT14I CCWWGG 1 cut(s) 801
EcoT22I ATGCAT 2 cut(s) 574, 1593
ErhI CCWWGG 1 cut(s) 801
Esp3I CGTCTC 1 cut(s) 735
FaqI GGGAC 1 cut(s) 1043
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 4 cut(s) 63, 263, 852, 1158
FokI GGATG 5 cut(s) 405, 701, 818, 1177, 1392
Fsp4HI GCNGC 4 cut(s) 63, 263, 852, 1158
FspBI CTAG 3 cut(s) 491, 1391, 1640
GlaI GCGC 2 cut(s) 679, 1607
GluI GCNGC 4 cut(s) 63, 263, 852, 1158
GsaI CCCAGC 1 cut(s) 1450
HaeII RGCGCY 1 cut(s) 1609
HaeIII GGCC 5 cut(s) 169, 319, 597, 795, 908
HapII CCGG 2 cut(s) 1229, 1284
HhaI GCGC 2 cut(s) 680, 1608
Hin6I GCGC 2 cut(s) 678, 1606
HinP1I GCGC 2 cut(s) 678, 1606
HincII GTYRAC 1 cut(s) 1311
HindII GTYRAC 1 cut(s) 1311
HindIII AAGCTT 3 cut(s) 835, 924, 1177
HinfI GANTC 3 cut(s) 1020, 1527, 1557
HpaII CCGG 2 cut(s) 1229, 1284
HphI GGTGA 6 cut(s) 109, 506, 1003, 1037, 1081, 1298
Hpy166II GTNNAC 6 cut(s) 333, 351, 1072, 1102, 1168, 1311
Hpy188I TCNGA 5 cut(s) 283, 568, 669, 1556, 1562
Hpy188III TCNNGA 6 cut(s) 142, 944, 1024, 1240, 1298, 1688
Hpy8I GTNNAC 6 cut(s) 333, 351, 1072, 1102, 1168, 1311
HpyAV CCTTC 4 cut(s) 356, 466, 1324, 1326
HpyCH4III ACNGT 7 cut(s) 148, 306, 389, 409, 640, 1522, 1630
HpyCH4IV ACGT 1 cut(s) 1056
HpyF10VI GCNNNNNNNGC 3 cut(s) 857, 878, 1372
HpyF3I CTNAG 2 cut(s) 291, 1299
HpySE526I ACGT 1 cut(s) 1056
HspAI GCGC 2 cut(s) 678, 1606
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
LguI GCTCTTC 1 cut(s) 687
LmnI GCTCC 5 cut(s) 617, 643, 793, 878, 1026
Lsp1109I GCAGC 3 cut(s) 274, 838, 1144
LweI GCATC 4 cut(s) 49, 559, 1007, 1578
MaeI CTAG 3 cut(s) 491, 1391, 1640
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 3 cut(s) 766, 1118, 1595
MalI GATC 6 cut(s) 203, 748, 1044, 1226, 1293, 1503
MboI GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
MflI RGATCY 3 cut(s) 746, 1224, 1291
MhlI GDGCHC 1 cut(s) 311
MlsI TGGCCA 1 cut(s) 795
MluNI TGGCCA 1 cut(s) 795
MlyI GAGTC 1 cut(s) 1536
MmeI TCCRAC 1 cut(s) 1504
Mox20I TGGCCA 1 cut(s) 795
Mph1103I ATGCAT 2 cut(s) 574, 1593
MroXI GAANNNNTTC 1 cut(s) 1293
MscI TGGCCA 1 cut(s) 795
MseI TTAA 4 cut(s) 207, 258, 1110, 1275
MslI CAYNNNNRTG 4 cut(s) 249, 1331, 1419, 1536
Msp20I TGGCCA 1 cut(s) 795
MspI CCGG 2 cut(s) 1229, 1284
MspR9I CCNGG 9 cut(s) 165, 277, 321, 441, 594, 792, 1229, 1230, 1575
MvaI CCWGG 7 cut(s) 165, 277, 321, 441, 594, 792, 1575
MwoI GCNNNNNNNGC 3 cut(s) 857, 878, 1372
NciI CCSGG 2 cut(s) 1229, 1230
NcoI CCATGG 1 cut(s) 801
NdeII GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
NlaIV GGNNCC 4 cut(s) 418, 480, 748, 1028
NmuCI GTSAC 2 cut(s) 766, 1595
NsiI ATGCAT 2 cut(s) 574, 1593
NspI RCATGY 2 cut(s) 383, 1070
NspV TTCGAA 1 cut(s) 630
OliI CACNNNNGTG 1 cut(s) 1331
PaeI GCATGC 1 cut(s) 383
PciI ACATGT 1 cut(s) 1066
PciSI GCTCTTC 1 cut(s) 687
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 2 cut(s) 1020, 1557
PflMI CCANNNNNTGG 3 cut(s) 440, 802, 1133
PinAI ACCGGT 1 cut(s) 1283
PkrI GCNGC 4 cut(s) 64, 264, 853, 1159
PleI GAGTC 1 cut(s) 1535
PpsI GAGTC 1 cut(s) 1535
PscI ACATGT 1 cut(s) 1066
PsiI TTATAA 2 cut(s) 960, 1491
Psp6I CCWGG 7 cut(s) 163, 275, 319, 439, 592, 790, 1573
PspFI CCCAGC 1 cut(s) 1446
PspGI CCWGG 7 cut(s) 163, 275, 319, 439, 592, 790, 1573
PspN4I GGNNCC 4 cut(s) 418, 480, 748, 1028
PspPI GGNCC 3 cut(s) 167, 287, 1136
PsuI RGATCY 3 cut(s) 746, 1224, 1291
RsaI GTAC 2 cut(s) 332, 1349
RsaNI GTAC 2 cut(s) 331, 1348
RseI CAYNNNNRTG 4 cut(s) 249, 1331, 1419, 1536
SapI GCTCTTC 1 cut(s) 687
SaqAI TTAA 4 cut(s) 207, 258, 1110, 1275
SatI GCNGC 4 cut(s) 63, 263, 852, 1158
Sau3AI GATC 6 cut(s) 201, 746, 1042, 1224, 1291, 1501
Sau96I GGNCC 3 cut(s) 167, 287, 1136
SchI GAGTC 1 cut(s) 1536
ScrFI CCNGG 9 cut(s) 165, 277, 321, 441, 594, 792, 1229, 1230, 1575
SduI GDGCHC 1 cut(s) 311
SfaNI GCATC 4 cut(s) 49, 559, 1007, 1578
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 2 cut(s) 287, 1136
SmaI CCCGGG 1 cut(s) 1230
SmiMI CAYNNNNRTG 4 cut(s) 249, 1331, 1419, 1536
SphI GCATGC 1 cut(s) 383
SsiI CCGC 1 cut(s) 63
SspMI CTAG 3 cut(s) 491, 1391, 1640
StyD4I CCNGG 9 cut(s) 163, 275, 319, 439, 592, 790, 1227, 1228, 1573
StyI CCWWGG 1 cut(s) 801
TaaI ACNGT 7 cut(s) 148, 306, 389, 409, 640, 1522, 1630
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 5 cut(s) 118, 630, 1197, 1207, 1239
TatI WGTACW 2 cut(s) 330, 1347
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 1020, 1557
Tru1I TTAA 4 cut(s) 207, 258, 1110, 1275
Tru9I TTAA 4 cut(s) 207, 258, 1110, 1275
TscAI CASTG 5 cut(s) 311, 394, 645, 1138, 1168
TseFI GTSAC 2 cut(s) 766, 1595
TseI GCWGC 3 cut(s) 262, 851, 1157
Tsp45I GTSAC 2 cut(s) 766, 1595
TspDTI ATGAA 6 cut(s) 233, 401, 705, 835, 918, 951
TspGWI ACGGA 1 cut(s) 759
TspMI CCCGGG 1 cut(s) 1228
TspRI CASTG 5 cut(s) 311, 394, 645, 1138, 1168
Van91I CCANNNNNTGG 3 cut(s) 440, 802, 1133
VpaK11BI GGWCC 2 cut(s) 287, 1136
XapI RAATTY 4 cut(s) 947, 962, 1271, 1671
XceI RCATGY 2 cut(s) 383, 1070
XcmI CCANNNNNNNNNTGG 1 cut(s) 273
XmaI CCCGGG 1 cut(s) 1228
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 3 cut(s) 491, 1391, 1640
Zsp2I ATGCAT 2 cut(s) 574, 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.