Belongs to the STXBP unc-18 SEC1 family
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G42700 AT2G42700 AT2G42700 |
| fragaria_vesca | FvH4_6g27600 FvH4_6g27600 |
| malus_domestica | MD11G1004500.v1.1 |
| prunus_persica | Prupe.3G115700_v2.0.a1 |
| pyrus_communis | pycom09g15450 |
| rosa_chinensis | RchiOBHm_Chr2g0130091 |
| rosa_laevigata | RLG00000019126 |
| rosa_multiflora | Rmu_co7958197.1_g000001 Rmu_co8394299.1_g000001 Rmu_sc0010460.1_g000011 |
| rosa_roxburghii | Rroxscaffold_2G00114090 |
| rosa_rugosa | Rorug02G0291000 |
| rosa_samantha | Rh2AG342800 Rh2BG350500 Rh2CG329900 Rh2DG369000 |
| rosa_wichuraiana | Rw2G027670 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2301 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 922 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 115, 1862 |
| AccI | GTMKAC | 2 cut(s) | 95, 253 |
| AccII | CGCG | 1 cut(s) | 1461 |
| AciI | CCGC | 2 cut(s) | 1459, 2514 |
| AclWI | GGATC | 3 cut(s) | 212, 709, 2267 |
| AcoI | YGGCCR | 1 cut(s) | 1626 |
| AcsI | RAATTY | 8 cut(s) | 211, 451, 826, 1167, 1296, 1363, 1849, 2186 |
| AcuI | CTGAAG | 5 cut(s) | 678, 1301, 1976, 2019, 2168 |
| AcyI | GRCGYC | 1 cut(s) | 1778 |
| AdeI | CACNNNGTG | 1 cut(s) | 884 |
| AfaI | GTAC | 4 cut(s) | 319, 772, 985, 2337 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 52, 115, 698, 788, 1132, 1862, 2410 |
| AflII | CTTAAG | 2 cut(s) | 766, 1996 |
| AflIII | ACRYGT | 2 cut(s) | 174, 921 |
| AjiI | CACGTC | 1 cut(s) | 1490 |
| AjnI | CCWGG | 5 cut(s) | 220, 805, 1492, 2338, 2445 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 1754, 1786, 1849, 1881, 2523, 2555 |
| Alw21I | GWGCWC | 2 cut(s) | 60, 296 |
| Alw26I | GTCTC | 3 cut(s) | 482, 893, 1982 |
| AlwI | GGATC | 3 cut(s) | 212, 709, 2267 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1595 |
| AoxI | GGCC | 4 cut(s) | 548, 1626, 1873, 2401 |
| ApaI | GGGCCC | 1 cut(s) | 1877 |
| ApeKI | GCWGC | 7 cut(s) | 845, 1589, 1592, 1595, 1703, 2158, 2371 |
| ApoI | RAATTY | 8 cut(s) | 211, 451, 826, 1167, 1296, 1363, 1849, 2186 |
| AseI | ATTAAT | 1 cut(s) | 1281 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 1227, 1365, 1452, 2535 |
| AspS9I | GGNCC | 5 cut(s) | 538, 549, 1419, 1873, 1874 |
| AsuC2I | CCSGG | 1 cut(s) | 753 |
| AsuII | TTCGAA | 1 cut(s) | 1323 |
| AvaII | GGWCC | 2 cut(s) | 538, 1419 |
| BaeGI | GKGCMC | 1 cut(s) | 1877 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 180, 213 |
| BanII | GRGCYC | 1 cut(s) | 1877 |
| BbsI | GAAGAC | 2 cut(s) | 2025, 2524 |
| Bbv12I | GWGCWC | 2 cut(s) | 60, 296 |
| BbvI | GCAGC | 7 cut(s) | 832, 1582, 1601, 1604, 1690, 2145, 2383 |
| BccI | CCATC | 9 cut(s) | 504, 782, 846, 847, 859, 1409, 1626, 1948, 2231 |
| BceAI | ACGGC | 1 cut(s) | 933 |
| BciT130I | CCWGG | 5 cut(s) | 222, 807, 1494, 2340, 2447 |
| BclI | TGATCA | 1 cut(s) | 240 |
| BcnI | CCSGG | 1 cut(s) | 753 |
| BcoDI | GTCTC | 3 cut(s) | 482, 893, 1982 |
| BfaI | CTAG | 3 cut(s) | 558, 1397, 2598 |
| BfmI | CTRYAG | 1 cut(s) | 2250 |
| BfrI | CTTAAG | 2 cut(s) | 766, 1996 |
| BisI | GCNGC | 8 cut(s) | 846, 1459, 1590, 1593, 1596, 1704, 2159, 2372 |
| BlsI | GCNGC | 8 cut(s) | 847, 1460, 1591, 1594, 1597, 1705, 2160, 2373 |
| BmcAI | AGTACT | 1 cut(s) | 772 |
| Bme1390I | CCNGG | 6 cut(s) | 222, 753, 807, 1494, 2340, 2447 |
| Bme18I | GGWCC | 2 cut(s) | 538, 1419 |
| BmgBI | CACGTC | 1 cut(s) | 1490 |
| BmgT120I | GGNCC | 5 cut(s) | 538, 549, 1419, 1873, 1874 |
| BmiI | GGNNCC | 3 cut(s) | 1420, 1867, 1875 |
| BmrFI | CCNGG | 6 cut(s) | 222, 753, 807, 1494, 2340, 2447 |
| BmrI | ACTGGG | 1 cut(s) | 1543 |
| BmsI | GCATC | 7 cut(s) | 76, 253, 373, 533, 649, 730, 1795 |
| BmuI | ACTGGG | 1 cut(s) | 1543 |
| BpiI | GAAGAC | 2 cut(s) | 2025, 2524 |
| BpmI | CTGGAG | 1 cut(s) | 1701 |
| Bpu14I | TTCGAA | 1 cut(s) | 1323 |
| BpuEI | CTTGAG | 4 cut(s) | 1091, 1248, 2006, 2478 |
| BpuMI | CCSGG | 1 cut(s) | 753 |
| Bsa29I | ATCGAT | 1 cut(s) | 271 |
| BsaBI | GATNNNNATC | 1 cut(s) | 267 |
| BsaHI | GRCGYC | 1 cut(s) | 1778 |
| BsaI | GGTCTC | 2 cut(s) | 893, 1982 |
| BsaJI | CCNNGG | 5 cut(s) | 142, 806, 1524, 1869, 2404 |
| BsaWI | WCCGGW | 1 cut(s) | 2521 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1886, 1916 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 52, 115, 698, 788, 1132, 1862, 2410 |
| Bse1I | ACTGG | 6 cut(s) | 528, 778, 1542, 1549, 1605, 2205 |
| Bse8I | GATNNNNATC | 1 cut(s) | 267 |
| BseBI | CCWGG | 5 cut(s) | 222, 807, 1494, 2340, 2447 |
| BseCI | ATCGAT | 1 cut(s) | 271 |
| BseDI | CCNNGG | 5 cut(s) | 142, 806, 1524, 1869, 2404 |
| BseJI | GATNNNNATC | 1 cut(s) | 267 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 52, 115, 698, 788, 1132, 1862, 2410 |
| BseMII | CTCAG | 2 cut(s) | 281, 1553 |
| BseNI | ACTGG | 6 cut(s) | 528, 778, 1542, 1549, 1605, 2205 |
| BseRI | GAGGAG | 2 cut(s) | 440, 1907 |
| BseSI | GKGCMC | 1 cut(s) | 1877 |
| BseXI | GCAGC | 7 cut(s) | 832, 1582, 1601, 1604, 1690, 2145, 2383 |
| BseYI | CCCAGC | 2 cut(s) | 89, 2587 |
| BsgI | GTGCAG | 2 cut(s) | 1581, 1671 |
| Bsh1236I | CGCG | 1 cut(s) | 1461 |
| BshFI | GGCC | 4 cut(s) | 550, 1628, 1875, 2403 |
| BshVI | ATCGAT | 1 cut(s) | 271 |
| BsiHKAI | GWGCWC | 2 cut(s) | 60, 296 |
| BsiSI | CCGG | 2 cut(s) | 753, 2522 |
| BslFI | GGGAC | 3 cut(s) | 391, 735, 1432 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 52, 115, 698, 788, 1132, 1862, 2410 |
| BsmAI | GTCTC | 3 cut(s) | 482, 893, 1982 |
| BsmFI | GGGAC | 3 cut(s) | 391, 735, 1432 |
| BsmI | GAATGC | 2 cut(s) | 211, 2243 |
| BsnI | GGCC | 4 cut(s) | 550, 1628, 1875, 2403 |
| Bso31I | GGTCTC | 2 cut(s) | 893, 1982 |
| Bsp119I | TTCGAA | 1 cut(s) | 1323 |
| Bsp120I | GGGCCC | 1 cut(s) | 1873 |
| Bsp1286I | GDGCHC | 3 cut(s) | 60, 296, 1877 |
| Bsp19I | CCATGG | 2 cut(s) | 142, 1524 |
| BspACI | CCGC | 2 cut(s) | 1459, 2514 |
| BspANI | GGCC | 4 cut(s) | 550, 1628, 1875, 2403 |
| BspCNI | CTCAG | 2 cut(s) | 282, 1554 |
| BspDI | ATCGAT | 1 cut(s) | 271 |
| BspFNI | CGCG | 1 cut(s) | 1461 |
| BspHI | TCATGA | 1 cut(s) | 391 |
| BspLI | GGNNCC | 3 cut(s) | 1420, 1867, 1875 |
| BspMAI | CTGCAG | 1 cut(s) | 2254 |
| BspPI | GGATC | 3 cut(s) | 212, 709, 2267 |
| BspQI | GCTCTTC | 1 cut(s) | 1458 |
| BspT104I | TTCGAA | 1 cut(s) | 1323 |
| BspTI | CTTAAG | 2 cut(s) | 766, 1996 |
| BspTNI | GGTCTC | 2 cut(s) | 893, 1982 |
| BsrI | ACTGG | 6 cut(s) | 528, 778, 1542, 1549, 1605, 2205 |
| BssECI | CCNNGG | 5 cut(s) | 142, 806, 1524, 1869, 2404 |
| BssNI | GRCGYC | 1 cut(s) | 1778 |
| BssT1I | CCWWGG | 3 cut(s) | 142, 1524, 2404 |
| Bst2UI | CCWGG | 5 cut(s) | 222, 807, 1494, 2340, 2447 |
| Bst4CI | ACNGT | 7 cut(s) | 193, 322, 901, 983, 1474, 2117, 2365 |
| Bst6I | CTCTTC | 5 cut(s) | 528, 579, 1458, 2207, 2362 |
| BstACI | GRCGYC | 1 cut(s) | 1778 |
| BstAFI | CTTAAG | 2 cut(s) | 766, 1996 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1595, 1604, 2249 |
| BstBI | TTCGAA | 1 cut(s) | 1323 |
| BstC8I | GCNNGC | 1 cut(s) | 2412 |
| BstDEI | CTNAG | 6 cut(s) | 82, 257, 290, 746, 1172, 1562 |
| BstDSI | CCRYGG | 2 cut(s) | 142, 1524 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2408 |
| BstFNI | CGCG | 1 cut(s) | 1461 |
| BstMAI | GTCTC | 3 cut(s) | 482, 893, 1982 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 353, 1595, 1604, 1894, 2249, 2498 |
| BstNI | CCWGG | 5 cut(s) | 222, 807, 1494, 2340, 2447 |
| BstNSI | RCATGY | 4 cut(s) | 178, 925, 2143, 2575 |
| BstSCI | CCNGG | 6 cut(s) | 220, 751, 805, 1492, 2338, 2445 |
| BstSFI | CTRYAG | 1 cut(s) | 2250 |
| BstSLI | GKGCMC | 1 cut(s) | 1877 |
| BstUI | CGCG | 1 cut(s) | 1461 |
| BstV1I | GCAGC | 7 cut(s) | 832, 1582, 1601, 1604, 1690, 2145, 2383 |
| BstV2I | GAAGAC | 2 cut(s) | 2025, 2524 |
| BstX2I | RGATCY | 1 cut(s) | 2272 |
| BstYI | RGATCY | 1 cut(s) | 2272 |
| Bsu15I | ATCGAT | 1 cut(s) | 271 |
| BsuRI | GGCC | 4 cut(s) | 550, 1628, 1875, 2403 |
| BsuTUI | ATCGAT | 1 cut(s) | 271 |
| BtgI | CCRYGG | 2 cut(s) | 142, 1524 |
| BtrI | CACGTC | 1 cut(s) | 1490 |
| BtsIMutI | CAGTG | 5 cut(s) | 535, 1535, 1598, 1654, 2272 |
| Cac8I | GCNNGC | 1 cut(s) | 2412 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1595 |
| CciI | TCATGA | 1 cut(s) | 391 |
| Cfr13I | GGNCC | 5 cut(s) | 538, 549, 1419, 1873, 1874 |
| ClaI | ATCGAT | 1 cut(s) | 271 |
| CseI | GACGC | 1 cut(s) | 1767 |
| CsiI | ACCWGGT | 1 cut(s) | 2338 |
| Csp6I | GTAC | 4 cut(s) | 318, 771, 984, 2336 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 882, 917 |
| CviQI | GTAC | 4 cut(s) | 318, 771, 984, 2336 |
| DdeI | CTNAG | 6 cut(s) | 82, 257, 290, 746, 1172, 1562 |
| DraI | TTTAAA | 2 cut(s) | 480, 2380 |
| DraIII | CACNNNGTG | 1 cut(s) | 884 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 922 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 922 |
| EaeI | YGGCCR | 1 cut(s) | 1626 |
| Eam1104I | CTCTTC | 5 cut(s) | 528, 579, 1458, 2207, 2362 |
| EarI | CTCTTC | 5 cut(s) | 528, 579, 1458, 2207, 2362 |
| Eco130I | CCWWGG | 3 cut(s) | 142, 1524, 2404 |
| Eco24I | GRGCYC | 1 cut(s) | 1877 |
| Eco31I | GGTCTC | 2 cut(s) | 893, 1982 |
| Eco32I | GATATC | 2 cut(s) | 51, 2527 |
| Eco47I | GGWCC | 2 cut(s) | 538, 1419 |
| Eco57I | CTGAAG | 5 cut(s) | 678, 1301, 1976, 2019, 2168 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2408 |
| EcoO109I | RGGNCCY | 3 cut(s) | 538, 1873, 1874 |
| EcoRII | CCWGG | 5 cut(s) | 220, 805, 1492, 2338, 2445 |
| EcoRV | GATATC | 2 cut(s) | 51, 2527 |
| EcoT14I | CCWWGG | 3 cut(s) | 142, 1524, 2404 |
| EcoT22I | ATGCAT | 1 cut(s) | 388 |
| EcoT38I | GRGCYC | 1 cut(s) | 1877 |
| ErhI | CCWWGG | 3 cut(s) | 142, 1524, 2404 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 842, 874, 1437, 1469, 1776, 1808 |
| FaqI | GGGAC | 3 cut(s) | 391, 735, 1432 |
| FauNDI | CATATG | 2 cut(s) | 358, 2278 |
| FbaI | TGATCA | 1 cut(s) | 240 |
| FblI | GTMKAC | 2 cut(s) | 95, 253 |
| Fnu4HI | GCNGC | 8 cut(s) | 846, 1459, 1590, 1593, 1596, 1704, 2159, 2372 |
| FriOI | GRGCYC | 1 cut(s) | 1877 |
| Fsp4HI | GCNGC | 8 cut(s) | 846, 1459, 1590, 1593, 1596, 1704, 2159, 2372 |
| FspBI | CTAG | 3 cut(s) | 558, 1397, 2598 |
| GluI | GCNGC | 8 cut(s) | 846, 1459, 1590, 1593, 1596, 1704, 2159, 2372 |
| GsaI | CCCAGC | 2 cut(s) | 93, 2591 |
| GsuI | CTGGAG | 1 cut(s) | 1701 |
| HaeIII | GGCC | 4 cut(s) | 550, 1628, 1875, 2403 |
| HapII | CCGG | 2 cut(s) | 753, 2522 |
| HgaI | GACGC | 1 cut(s) | 1767 |
| Hin1I | GRCGYC | 1 cut(s) | 1778 |
| HincII | GTYRAC | 2 cut(s) | 979, 2101 |
| HindII | GTYRAC | 2 cut(s) | 979, 2101 |
| HindIII | AAGCTT | 2 cut(s) | 1224, 2303 |
| HpaII | CCGG | 2 cut(s) | 753, 2522 |
| Hpy166II | GTNNAC | 6 cut(s) | 96, 254, 953, 979, 986, 2101 |
| Hpy8I | GTNNAC | 6 cut(s) | 96, 254, 953, 979, 986, 2101 |
| HpyCH4III | ACNGT | 7 cut(s) | 193, 322, 901, 983, 1474, 2117, 2365 |
| HpyCH4IV | ACGT | 4 cut(s) | 283, 1489, 2030, 2334 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 353, 1595, 1604, 1894, 2249, 2498 |
| HpyF3I | CTNAG | 6 cut(s) | 82, 257, 290, 746, 1172, 1562 |
| HpySE526I | ACGT | 4 cut(s) | 283, 1489, 2030, 2334 |
| Hsp92I | GRCGYC | 1 cut(s) | 1778 |
| Ksp22I | TGATCA | 1 cut(s) | 240 |
| LguI | GCTCTTC | 1 cut(s) | 1458 |
| LmnI | GCTCC | 6 cut(s) | 55, 119, 427, 1818, 1894, 2155 |
| Lsp1109I | GCAGC | 7 cut(s) | 832, 1582, 1601, 1604, 1690, 2145, 2383 |
| LweI | GCATC | 7 cut(s) | 76, 253, 373, 533, 649, 730, 1795 |
| MabI | ACCWGGT | 1 cut(s) | 2338 |
| MaeI | CTAG | 3 cut(s) | 558, 1397, 2598 |
| MaeII | ACGT | 4 cut(s) | 283, 1489, 2030, 2334 |
| MaeIII | GTNAC | 6 cut(s) | 16, 132, 513, 1779, 2135, 2162 |
| MfeI | CAATTG | 1 cut(s) | 1898 |
| MflI | RGATCY | 1 cut(s) | 2272 |
| MhlI | GDGCHC | 3 cut(s) | 60, 296, 1877 |
| MlyI | GAGTC | 4 cut(s) | 156, 687, 1705, 2224 |
| MmeI | TCCRAC | 4 cut(s) | 477, 1221, 2133, 2198 |
| Mph1103I | ATGCAT | 1 cut(s) | 388 |
| MroXI | GAANNNNTTC | 4 cut(s) | 1227, 1365, 1452, 2535 |
| MseI | TTAA | 6 cut(s) | 479, 767, 1281, 1664, 1997, 2379 |
| MslI | CAYNNNNRTG | 3 cut(s) | 396, 738, 1427 |
| MspA1I | CMGCKG | 1 cut(s) | 1595 |
| MspCI | CTTAAG | 2 cut(s) | 766, 1996 |
| MspI | CCGG | 2 cut(s) | 753, 2522 |
| MspR9I | CCNGG | 6 cut(s) | 222, 753, 807, 1494, 2340, 2447 |
| MunI | CAATTG | 1 cut(s) | 1898 |
| Mva1269I | GAATGC | 2 cut(s) | 211, 2243 |
| MvaI | CCWGG | 5 cut(s) | 222, 807, 1494, 2340, 2447 |
| MvnI | CGCG | 1 cut(s) | 1461 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 353, 1595, 1604, 1894, 2249, 2498 |
| NciI | CCSGG | 1 cut(s) | 753 |
| NcoI | CCATGG | 2 cut(s) | 142, 1524 |
| NdeI | CATATG | 2 cut(s) | 358, 2278 |
| NlaIV | GGNNCC | 3 cut(s) | 1420, 1867, 1875 |
| NmeAIII | GCCGAG | 1 cut(s) | 526 |
| NmuCI | GTSAC | 5 cut(s) | 16, 513, 1779, 2135, 2162 |
| NsiI | ATGCAT | 1 cut(s) | 388 |
| NspI | RCATGY | 4 cut(s) | 178, 925, 2143, 2575 |
| NspV | TTCGAA | 1 cut(s) | 1323 |
| PagI | TCATGA | 1 cut(s) | 391 |
| PciI | ACATGT | 2 cut(s) | 174, 921 |
| PciSI | GCTCTTC | 1 cut(s) | 1458 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 952 |
| PctI | GAATGC | 2 cut(s) | 211, 2243 |
| PdmI | GAANNNNTTC | 4 cut(s) | 1227, 1365, 1452, 2535 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 115, 1862 |
| PfoI | TCCNGGA | 2 cut(s) | 220, 751 |
| PkrI | GCNGC | 8 cut(s) | 847, 1460, 1591, 1594, 1597, 1705, 2160, 2373 |
| PleI | GAGTC | 4 cut(s) | 155, 686, 1704, 2223 |
| PpsI | GAGTC | 4 cut(s) | 155, 686, 1704, 2223 |
| PpuMI | RGGWCCY | 1 cut(s) | 538 |
| PscI | ACATGT | 2 cut(s) | 174, 921 |
| PshBI | ATTAAT | 1 cut(s) | 1281 |
| PsiI | TTATAA | 1 cut(s) | 2301 |
| Psp5II | RGGWCCY | 1 cut(s) | 538 |
| Psp6I | CCWGG | 5 cut(s) | 220, 805, 1492, 2338, 2445 |
| PspFI | CCCAGC | 2 cut(s) | 89, 2587 |
| PspGI | CCWGG | 5 cut(s) | 220, 805, 1492, 2338, 2445 |
| PspN4I | GGNNCC | 3 cut(s) | 1420, 1867, 1875 |
| PspOMI | GGGCCC | 1 cut(s) | 1873 |
| PspPI | GGNCC | 5 cut(s) | 538, 549, 1419, 1873, 1874 |
| PspPPI | RGGWCCY | 1 cut(s) | 538 |
| PstI | CTGCAG | 1 cut(s) | 2254 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1595 |
| PsuI | RGATCY | 1 cut(s) | 2272 |
| PvuII | CAGCTG | 1 cut(s) | 1595 |
| RsaI | GTAC | 4 cut(s) | 319, 772, 985, 2337 |
| RsaNI | GTAC | 4 cut(s) | 318, 771, 984, 2336 |
| RseI | CAYNNNNRTG | 3 cut(s) | 396, 738, 1427 |
| SapI | GCTCTTC | 1 cut(s) | 1458 |
| SaqAI | TTAA | 6 cut(s) | 479, 767, 1281, 1664, 1997, 2379 |
| SatI | GCNGC | 8 cut(s) | 846, 1459, 1590, 1593, 1596, 1704, 2159, 2372 |
| Sau96I | GGNCC | 5 cut(s) | 538, 549, 1419, 1873, 1874 |
| ScaI | AGTACT | 1 cut(s) | 772 |
| SchI | GAGTC | 4 cut(s) | 156, 687, 1705, 2224 |
| ScrFI | CCNGG | 6 cut(s) | 222, 753, 807, 1494, 2340, 2447 |
| SduI | GDGCHC | 3 cut(s) | 60, 296, 1877 |
| SexAI | ACCWGGT | 1 cut(s) | 2338 |
| SfaNI | GCATC | 7 cut(s) | 76, 253, 373, 533, 649, 730, 1795 |
| SfcI | CTRYAG | 1 cut(s) | 2250 |
| SfuI | TTCGAA | 1 cut(s) | 1323 |
| SinI | GGWCC | 2 cut(s) | 538, 1419 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 396, 738, 1427 |
| SmlI | CTYRAG | 6 cut(s) | 766, 1106, 1263, 1985, 1996, 2493 |
| SmoI | CTYRAG | 6 cut(s) | 766, 1106, 1263, 1985, 1996, 2493 |
| SsiI | CCGC | 2 cut(s) | 1459, 2514 |
| SspI | AATATT | 2 cut(s) | 873, 1662 |
| SspMI | CTAG | 3 cut(s) | 558, 1397, 2598 |
| StyD4I | CCNGG | 6 cut(s) | 220, 751, 805, 1492, 2338, 2445 |
| StyI | CCWWGG | 3 cut(s) | 142, 1524, 2404 |
| TaaI | ACNGT | 7 cut(s) | 193, 322, 901, 983, 1474, 2117, 2365 |
| TaiI | ACGT | 4 cut(s) | 286, 1492, 2033, 2337 |
| TaqI | TCGA | 6 cut(s) | 33, 271, 1128, 1323, 2478, 2529 |
| TatI | WGTACW | 2 cut(s) | 317, 770 |
| TauI | GCSGC | 1 cut(s) | 1461 |
| Tru1I | TTAA | 6 cut(s) | 479, 767, 1281, 1664, 1997, 2379 |
| Tru9I | TTAA | 6 cut(s) | 479, 767, 1281, 1664, 1997, 2379 |
| TscAI | CASTG | 5 cut(s) | 535, 1542, 1605, 1654, 2272 |
| TseFI | GTSAC | 5 cut(s) | 16, 513, 1779, 2135, 2162 |
| TseI | GCWGC | 7 cut(s) | 845, 1589, 1592, 1595, 1703, 2158, 2371 |
| Tsp45I | GTSAC | 5 cut(s) | 16, 513, 1779, 2135, 2162 |
| TspGWI | ACGGA | 2 cut(s) | 140, 299 |
| TspRI | CASTG | 5 cut(s) | 535, 1542, 1605, 1654, 2272 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 115, 1862 |
| Vha464I | CTTAAG | 2 cut(s) | 766, 1996 |
| VpaK11BI | GGWCC | 2 cut(s) | 538, 1419 |
| VspI | ATTAAT | 1 cut(s) | 1281 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2408 |
| XapI | RAATTY | 8 cut(s) | 211, 451, 826, 1167, 1296, 1363, 1849, 2186 |
| XceI | RCATGY | 4 cut(s) | 178, 925, 2143, 2575 |
| XmiI | GTMKAC | 2 cut(s) | 95, 253 |
| XmnI | GAANNNNTTC | 4 cut(s) | 1227, 1365, 1452, 2535 |
| XspI | CTAG | 3 cut(s) | 558, 1397, 2598 |
| ZrmI | AGTACT | 1 cut(s) | 772 |
| Zsp2I | ATGCAT | 1 cut(s) | 388 |