RLG00000019126

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011362
Physical Location & Seq
Forward (+)
46957509 .. 46974420
16912 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019126

Sequence Viewer

Length: 2907 bp
ATGGAACGGGATCCTGCTAGTACTGCTGATTCTCAGGCAAACAAGGAAGCAGCTGATGAACGTTCTGTTTTCGTTGGCAATGTGGGAGAAAGGAACAAAAAGAAGGATAAGTTACATTTTGTTGTACTTGAGCTGGATGTTGGTGTCAGCTTGTGTAACAAAGGGAATTCACACTATAGCAATGGTGGCCTGGAAAGAGAGTCCAACAATCCATACCATCGGCAAGCAGTAACTGTGTGGGATGCAACTGCAGCTGATCCTAAATTGCTGGGTAAACACGGTATCGAGAAACAACCGTTTCAGCCCCCTGATTTCATTGCTGCAACTGGTACTGAGAAAATTAGACAGATATCGGAGCACATTGAAGGTTCTGTTCTTTACCTAGATGCTGGATCTACAGAGAGTTTCCAGTACATTGGAGCTTTTCCTCTCTTACTCAACCATGGAGTTCGTGCCATTTGCAGTTTGGAAAATATGTCTTCTCTTGATGCGGTAGTTGATTGGAATGCAAATTCTGATCCTGATAGGAAAGTCGTGGTGGTAACAACCCGTTTGCTAAGTGATGCACATCGATATATATTACGTTGTCTTAGCACTCATCCAGCTGTCCATTGTTGTACTATATTCACATCTATCTCAGAGATAGCCCATTCAGCATATCCTGATTCACCTCTGGGACCAGATGCATTTCATGAATATGAATCCTTGCTTGTCCAAGATTATGAGGAACTTGTCAAGAAAGGTGTAAAGAAACCCTTACAGCCTGGTGTTGGCAACTTGAAGGATAATATAGATTTAGGAGATGAAGGATGGTCACAGCGTGCTCCGAGTGAAGAGGACAAATCTCAGCCCCAAGCTAGTTCAACTGCAAGAGATTCCTATGGAGAGAATCTGATTTCTGATACAGAAAAAGTAGGGCAAAATTTGGTTGTTTCTGTGCGTCACTTCCCAATGATTATCTGTCCTTTTTCACCAAGAGTCTTTGTTTTACCTCCTGAGGGATCAGTTGCTGAAGCATACTTATCAGCTAAACATGAGGATGCCCTTAGTCCTGGATTACCCTCCTTAAGTAATGGGTTGCTTTCTGATGGTGATGATATTCCTCCTGGGGCAACTCTTACAGCAAATTTTCTTTACCATTTTGCGGCCAAGTTGGACCTGAAGATGGAGATATTTTCACTTGGTGATCTCTCAAAAACTGTGGGGAAGATGTTGACAGACATGTCAAGTCTTTATGATGTTGGTCGCCGTAAACGATCAGCAGGGCTATTACTCATTGACCGTACACTAGATCTTCTTACTCCATGCTGTCATGGAGATTCACTTGTTGACCGTGTGTTTTCATCCTTGCCTCGTAGGGAAAATACAGCATCCTATACTCAAATAAAAACCTCAAAAAGCCAACTAAAACAGGGCCCGTCAAACCTGGAACGTGCTTCTCTTGATGTTCAGATACCACTTGCTAAAATTCTAAGTGAAGAAGATTGCAAGATAGAGAACTTTCGGCTATTAGAAAGCATTGAAGCTTTTCTATGTGGGTGGGATTCTGGAAACTCTGCTTCTCAAGTTTTAGATCTGAGCAATCTCAAGAACAAAATTCATGATGAGAAGCTTCCCCAGTCAGGGAATGAGCTTCTAAGAGGGTCTCTTGTGTCCACTGAAAGTTTTCGAGGAACACCATATTTGGAAACCCTACTAGATAGGAAAACCAAAGATGGGACCATACTAGTAAAGAAGTGGCTTCAAGAAGCCCTTCGTCGGGAAAATATCTCCGTGAATGTGAAATCCCGTCCAGGTTTTGCTACAAAGTCAGAGTTGCAAGCTATGACAAAAGCACTGGCCAAATCCCAGTCATCTGTACTGAAAAACAAAGGAATTATTCAGTTGGCAGCGGCTGTACTGACTGCACTTGATGAATCTCATTCATCCAGATGGGAAGCATTTAGCAGTGCAGAGAAAATATTAAGTGTAAGTGCTGAAGACACAAGCCAGAGTCTTGCTGTGCAAATTGGTGATTTTATTAATAAGAGTGCTTTGTTGGGATTGCATGGACAGAAGAATGGAAAATTTGGGGTCTCCCAAGGGGTACTTTCTTTTCAGGATACTTTGTATCTTATGATTTCTGGATATATATTAGCTGGTGAGAATTTCCCAACAGCTGGGAATGATGGCCCCTTTTCTTGGCAAGAGGAGCAACTATTGAAAGATTCTATTGTTGAAGCAATTCTTGAAAACCCTTCAATAGCAAGACTGAAGTTTCTTCATGGCCTAATGGAAGAGCTTGAGACCAACTTAAGCAGGATCAAATCAGAGGAAAGCAAGGGGACATCCTCAGATCAAATAGATATCGATGATCTTGATGACGATCAGTGGGGTAAATGGGGTGATGAAGATGTTGATGATACAAATAGCAGTAAAGAGAAAGTGTATGATGACATGCAGCTGAAGTTGGAGTTGCGTGATAGGGTGGATAACCTTTTCAAATTCCTTCACAAGTTATCCAGTTTGAAGAGTCGAAACATACCATTGAAGGATGGGGCTTTAGATTCGGACAATAATTTCAGTGGAGATCCATATGCTAGTAGAGGATTACTTTATAAGCTTCTAACACGGGTATTGAGCAAGAATGATGTTCCTGGTTTGGAGTATCACTCTTCTACTGTGGGGCAACTATTTAAAAGTGGCTTCAGAAGATTTGGCCTTGGGCAGGCAAAACCAAGTTTAGCCGATCAAAATGTCATCCTTGTATTTGTTATTGGGGGCATCAATGGTGTGGAGGTTCGCGAAGCTCAAGAGGCATTATCTGAAAGTGGAAGACCGGATATAGAAATGATTCTTGGTGGAACAACTCTCCTAACTCCTGACGACATGCTTGACTTGCTACTGGGGAAATCTAGTTATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006508 GO:0006624 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016485 GO:0019538 GO:0043170 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0051604 GO:0071704 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec1

Protein Analysis

969

Amino Acids

106.39

Weight (kDa)

5.21

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 778 - 959 9.3e-08 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42700 AT2G42700 AT2G42700
fragaria_vesca FvH4_6g27600 FvH4_6g27600
malus_domestica MD11G1004500.v1.1
prunus_persica Prupe.3G115700_v2.0.a1
pyrus_communis pycom09g15450
rosa_chinensis RchiOBHm_Chr2g0130091
rosa_laevigata RLG00000019126
rosa_multiflora Rmu_co7958197.1_g000001 Rmu_co8394299.1_g000001 Rmu_sc0010460.1_g000011
rosa_roxburghii Rroxscaffold_2G00114090
rosa_rugosa Rorug02G0291000
rosa_samantha Rh2AG342800 Rh2BG350500 Rh2CG329900 Rh2DG369000
rosa_wichuraiana Rw2G027670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2598
AasI GACNNNNNNGTC 1 cut(s) 1222
AccB7I CCANNNNNTGG 1 cut(s) 2159
AccII CGCG 1 cut(s) 2784
AciI CCGC 3 cut(s) 491, 1145, 1892
AclI AACGTT 1 cut(s) 61
AclWI GGATC 8 cut(s) 5, 18, 251, 400, 512, 1009, 2309, 2564
AcoI YGGCCR 2 cut(s) 1146, 1839
AcsI RAATTY 9 cut(s) 166, 511, 922, 1126, 1467, 1596, 2066, 2146, 2483
AcuI CTGAAG 6 cut(s) 1032, 1181, 1998, 2273, 2465, 2671
AdeI CACNNNGTG 2 cut(s) 821, 1184
AfaI GTAC 9 cut(s) 22, 126, 331, 413, 619, 1285, 1860, 1899, 2088
AflII CTTAAG 2 cut(s) 1066, 2293
AflIII ACRYGT 1 cut(s) 1221
AhlI ACTAGT 1 cut(s) 1726
AjnI CCWGG 7 cut(s) 189, 763, 1051, 1105, 1425, 1792, 2635
AjuI GAANNNNNNNTTGG 2 cut(s) 2820, 2852
Alw21I GWGCWC 2 cut(s) 360, 826
Alw26I GTCTC 3 cut(s) 1650, 2080, 2279
AlwI GGATC 8 cut(s) 5, 18, 251, 400, 512, 1009, 2309, 2564
AlwNI CAGNNNCTG 3 cut(s) 233, 1010, 1895
AoxI GGCC 7 cut(s) 187, 1146, 1414, 1839, 2170, 2266, 2698
ApaI GGGCCC 1 cut(s) 1418
ApeKI GCWGC 5 cut(s) 50, 251, 320, 1889, 2440
ApoI RAATTY 9 cut(s) 166, 511, 922, 1126, 1467, 1596, 2066, 2146, 2483
AseI ATTAAT 1 cut(s) 2022
Asp700I GAANNNNTTC 5 cut(s) 1527, 1665, 1752, 2223, 2832
AspS9I GGNCC 6 cut(s) 677, 1156, 1414, 1415, 1719, 2171
AsuHPI GGTGA 7 cut(s) 660, 963, 1103, 1196, 2024, 2153, 2396
AvaII GGWCC 3 cut(s) 677, 1156, 1719
AxyI CCTNAGG 1 cut(s) 996
BaeGI GKGCMC 1 cut(s) 1418
BalI TGGCCA 1 cut(s) 1841
BamHI GGATCC 1 cut(s) 10
BanII GRGCYC 1 cut(s) 1418
BbsI GAAGAC 3 cut(s) 471, 1986, 2821
Bbv12I GWGCWC 2 cut(s) 360, 826
BbvI GCAGC 5 cut(s) 62, 263, 307, 1901, 2452
BccI CCATC 8 cut(s) 225, 804, 1082, 1159, 1709, 1926, 2162, 2528
BceAI ACGGC 1 cut(s) 1233
BciT130I CCWGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
BciVI GTATCC 1 cut(s) 2095
BcoDI GTCTC 3 cut(s) 1650, 2080, 2279
BcuI ACTAGT 1 cut(s) 1726
BfaI CTAG 8 cut(s) 18, 383, 858, 1289, 1697, 1727, 2580, 2895
BfmI CTRYAG 3 cut(s) 175, 249, 396
BfrI CTTAAG 2 cut(s) 1066, 2293
BfuI GTATCC 1 cut(s) 2095
BglII AGATCT 2 cut(s) 1291, 1573
BisI GCNGC 7 cut(s) 51, 252, 321, 1146, 1890, 1893, 2441
BlsI GCNGC 7 cut(s) 52, 253, 322, 1147, 1891, 1894, 2442
BmcAI AGTACT 1 cut(s) 22
Bme1390I CCNGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
Bme18I GGWCC 3 cut(s) 677, 1156, 1719
BmgT120I GGNCC 6 cut(s) 677, 1156, 1414, 1415, 1719, 2171
BmiI GGNNCC 5 cut(s) 12, 678, 1416, 1720, 2173
BmrFI CCNGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
BmrI ACTGGG 3 cut(s) 1612, 1843, 2894
BmsI GCATC 8 cut(s) 232, 376, 478, 553, 673, 1030, 1379, 2772
BmuI ACTGGG 3 cut(s) 1612, 1843, 2894
BpiI GAAGAC 3 cut(s) 471, 1986, 2821
BplI GAGNNNNNCTC 2 cut(s) 2636, 2668
BpuEI CTTGAG 5 cut(s) 149, 1548, 1571, 2303, 2775
Bsa29I ATCGAT 2 cut(s) 571, 2349
BsaBI GATNNNNATC 2 cut(s) 567, 2364
BsaI GGTCTC 3 cut(s) 1650, 2080, 2279
BsaJI CCNNGG 4 cut(s) 442, 1106, 2080, 2701
BsaWI WCCGGW 1 cut(s) 2818
Bse1I ACTGG 7 cut(s) 331, 409, 1618, 1842, 1849, 2502, 2889
Bse21I CCTNAGG 1 cut(s) 996
Bse3DI GCAATG 3 cut(s) 85, 187, 315
Bse8I GATNNNNATC 2 cut(s) 567, 2364
BseBI CCWGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
BseCI ATCGAT 2 cut(s) 571, 2349
BseDI CCNNGG 4 cut(s) 442, 1106, 2080, 2701
BseJI GATNNNNATC 2 cut(s) 567, 2364
BseMI GCAATG 3 cut(s) 85, 187, 315
BseMII CTCAG 7 cut(s) 47, 324, 651, 860, 987, 1568, 2346
BseNI ACTGG 7 cut(s) 331, 409, 1618, 1842, 1849, 2502, 2889
BseRI GAGGAG 1 cut(s) 2204
BseSI GKGCMC 1 cut(s) 1418
BseXI GCAGC 5 cut(s) 62, 263, 307, 1901, 2452
BseYI CCCAGC 2 cut(s) 268, 2159
BsgI GTGCAG 2 cut(s) 1890, 1971
Bsh1236I CGCG 1 cut(s) 2784
BshFI GGCC 7 cut(s) 189, 1148, 1416, 1841, 2172, 2268, 2700
BshVI ATCGAT 2 cut(s) 571, 2349
BsiHKAI GWGCWC 2 cut(s) 360, 826
BsiSI CCGG 1 cut(s) 2819
BslFI GGGAC 3 cut(s) 690, 1732, 2338
BsmAI GTCTC 3 cut(s) 1650, 2080, 2279
BsmFI GGGAC 3 cut(s) 690, 1732, 2338
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 7 cut(s) 189, 1148, 1416, 1841, 2172, 2268, 2700
Bso31I GGTCTC 3 cut(s) 1650, 2080, 2279
Bsp120I GGGCCC 1 cut(s) 1414
Bsp1286I GDGCHC 3 cut(s) 360, 826, 1418
Bsp19I CCATGG 1 cut(s) 442
Bsp68I TCGCGA 1 cut(s) 2784
BspACI CCGC 3 cut(s) 491, 1145, 1892
BspANI GGCC 7 cut(s) 189, 1148, 1416, 1841, 2172, 2268, 2700
BspCNI CTCAG 7 cut(s) 46, 325, 650, 859, 988, 1569, 2345
BspDI ATCGAT 2 cut(s) 571, 2349
BspFNI CGCG 1 cut(s) 2784
BspHI TCATGA 2 cut(s) 691, 1600
BspLI GGNNCC 5 cut(s) 12, 678, 1416, 1720, 2173
BspMAI CTGCAG 1 cut(s) 253
BspPI GGATC 8 cut(s) 5, 18, 251, 400, 512, 1009, 2309, 2564
BspQI GCTCTTC 1 cut(s) 2271
BspTI CTTAAG 2 cut(s) 1066, 2293
BspTNI GGTCTC 3 cut(s) 1650, 2080, 2279
BsrDI GCAATG 3 cut(s) 85, 187, 315
BsrI ACTGG 7 cut(s) 331, 409, 1618, 1842, 1849, 2502, 2889
BssECI CCNNGG 4 cut(s) 442, 1106, 2080, 2701
BssT1I CCWWGG 3 cut(s) 442, 2080, 2701
Bst2UI CCWGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
Bst4CI ACNGT 7 cut(s) 235, 281, 297, 1201, 1283, 1334, 2662
Bst6I CTCTTC 4 cut(s) 828, 2271, 2504, 2659
BstAFI CTTAAG 2 cut(s) 1066, 2293
BstC8I GCNNGC 4 cut(s) 225, 822, 1821, 2709
BstDSI CCRYGG 1 cut(s) 442
BstENI CCTNNNNNAGG 1 cut(s) 2705
BstFNI CGCG 1 cut(s) 2784
BstMAI GTCTC 3 cut(s) 1650, 2080, 2279
BstMWI GCNNNNNNNGC 7 cut(s) 23, 186, 251, 653, 2191, 2795, 2878
BstNI CCWGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
BstNSI RCATGY 3 cut(s) 1225, 2440, 2872
BstSCI CCNGG 7 cut(s) 189, 763, 1051, 1105, 1425, 1792, 2635
BstSFI CTRYAG 3 cut(s) 175, 249, 396
BstSLI GKGCMC 1 cut(s) 1418
BstUI CGCG 1 cut(s) 2784
BstV1I GCAGC 5 cut(s) 62, 263, 307, 1901, 2452
BstV2I GAAGAC 3 cut(s) 471, 1986, 2821
BstX2I RGATCY 5 cut(s) 10, 392, 1291, 1573, 2569
BstXI CCANNNNNNTGG 1 cut(s) 416
BstYI RGATCY 5 cut(s) 10, 392, 1291, 1573, 2569
Bsu15I ATCGAT 2 cut(s) 571, 2349
Bsu36I CCTNAGG 1 cut(s) 996
BsuI GTATCC 1 cut(s) 2095
BsuRI GGCC 7 cut(s) 189, 1148, 1416, 1841, 2172, 2268, 2700
BsuTUI ATCGAT 2 cut(s) 571, 2349
BtgI CCRYGG 1 cut(s) 442
BtsI GCAGTG 1 cut(s) 1954
BtsIMutI CAGTG 5 cut(s) 1656, 1835, 1954, 2375, 2569
BtuMI TCGCGA 1 cut(s) 2784
Cac8I GCNNGC 4 cut(s) 225, 822, 1821, 2709
CaiI CAGNNNCTG 3 cut(s) 233, 1010, 1895
CciI TCATGA 2 cut(s) 691, 1600
Cfr13I GGNCC 6 cut(s) 677, 1156, 1414, 1415, 1719, 2171
ClaI ATCGAT 2 cut(s) 571, 2349
CseI GACGC 1 cut(s) 929
Csp6I GTAC 9 cut(s) 21, 125, 330, 412, 618, 1284, 1859, 1898, 2087
CspCI CAANNNNNGTGG 2 cut(s) 1182, 1217
CviQI GTAC 9 cut(s) 21, 125, 330, 412, 618, 1284, 1859, 1898, 2087
DraI TTTAAA 1 cut(s) 2677
DraIII CACNNNGTG 2 cut(s) 821, 1184
DrdI GACNNNNNNGTC 1 cut(s) 1222
DseDI GACNNNNNNGTC 1 cut(s) 1222
EaeI YGGCCR 2 cut(s) 1146, 1839
Eam1104I CTCTTC 4 cut(s) 828, 2271, 2504, 2659
EarI CTCTTC 4 cut(s) 828, 2271, 2504, 2659
Eco130I CCWWGG 3 cut(s) 442, 2080, 2701
Eco24I GRGCYC 1 cut(s) 1418
Eco31I GGTCTC 3 cut(s) 1650, 2080, 2279
Eco32I GATATC 2 cut(s) 351, 2347
Eco47I GGWCC 3 cut(s) 677, 1156, 1719
Eco57I CTGAAG 6 cut(s) 1032, 1181, 1998, 2273, 2465, 2671
Eco81I CCTNAGG 1 cut(s) 996
EcoNI CCTNNNNNAGG 1 cut(s) 2705
EcoO109I RGGNCCY 1 cut(s) 1414
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 7 cut(s) 189, 763, 1051, 1105, 1425, 1792, 2635
EcoRV GATATC 2 cut(s) 351, 2347
EcoT14I CCWWGG 3 cut(s) 442, 2080, 2701
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 1418
ErhI CCWWGG 3 cut(s) 442, 2080, 2701
FalI AAGNNNNNCTT 8 cut(s) 740, 772, 1737, 1769, 2073, 2105, 2211, 2243
FaqI GGGAC 3 cut(s) 690, 1732, 2338
FauNDI CATATG 1 cut(s) 2575
Fnu4HI GCNGC 7 cut(s) 51, 252, 321, 1146, 1890, 1893, 2441
FriOI GRGCYC 1 cut(s) 1418
Fsp4HI GCNGC 7 cut(s) 51, 252, 321, 1146, 1890, 1893, 2441
FspBI CTAG 8 cut(s) 18, 383, 858, 1289, 1697, 1727, 2580, 2895
GluI GCNGC 7 cut(s) 51, 252, 321, 1146, 1890, 1893, 2441
GsaI CCCAGC 2 cut(s) 272, 2163
HaeIII GGCC 7 cut(s) 189, 1148, 1416, 1841, 2172, 2268, 2700
HapII CCGG 1 cut(s) 2819
HgaI GACGC 1 cut(s) 929
HincII GTYRAC 2 cut(s) 1215, 1330
HindII GTYRAC 2 cut(s) 1215, 1330
HindIII AAGCTT 3 cut(s) 1524, 1610, 2600
HpaII CCGG 1 cut(s) 2819
HphI GGTGA 7 cut(s) 660, 963, 1103, 1196, 2024, 2153, 2396
Hpy166II GTNNAC 6 cut(s) 275, 1215, 1253, 1286, 1330, 1656
Hpy8I GTNNAC 6 cut(s) 275, 1215, 1253, 1286, 1330, 1656
Hpy99I CGWCG 1 cut(s) 1761
HpyAV CCTTC 8 cut(s) 97, 359, 775, 800, 1763, 2247, 2498, 2524
HpyCH4III ACNGT 7 cut(s) 235, 281, 297, 1201, 1283, 1334, 2662
HpyCH4IV ACGT 3 cut(s) 61, 583, 1432
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 186, 251, 653, 2191, 2795, 2878
HpySE526I ACGT 3 cut(s) 61, 583, 1432
LguI GCTCTTC 1 cut(s) 2271
LmnI GCTCC 4 cut(s) 355, 419, 829, 2191
Lsp1109I GCAGC 5 cut(s) 62, 263, 307, 1901, 2452
LweI GCATC 8 cut(s) 232, 376, 478, 553, 673, 1030, 1379, 2772
MaeI CTAG 8 cut(s) 18, 383, 858, 1289, 1697, 1727, 2580, 2895
MaeII ACGT 3 cut(s) 61, 583, 1432
MaeIII GTNAC 6 cut(s) 111, 155, 229, 541, 813, 941
MflI RGATCY 5 cut(s) 10, 392, 1291, 1573, 2569
MhlI GDGCHC 3 cut(s) 360, 826, 1418
MlsI TGGCCA 1 cut(s) 1841
MluNI TGGCCA 1 cut(s) 1841
MlyI GAGTC 4 cut(s) 209, 987, 2002, 2521
MmeI TCCRAC 3 cut(s) 228, 1134, 2430
Mox20I TGGCCA 1 cut(s) 1841
Mph1103I ATGCAT 1 cut(s) 688
MroXI GAANNNNTTC 5 cut(s) 1527, 1665, 1752, 2223, 2832
MscI TGGCCA 1 cut(s) 1841
MseI TTAA 5 cut(s) 1067, 1964, 2022, 2294, 2676
MslI CAYNNNNRTG 3 cut(s) 696, 1038, 1930
Msp20I TGGCCA 1 cut(s) 1841
MspA1I CMGCKG 6 cut(s) 53, 254, 605, 1892, 2159, 2443
MspCI CTTAAG 2 cut(s) 1066, 2293
MspI CCGG 1 cut(s) 2819
MspR9I CCNGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
MvnI CGCG 1 cut(s) 2784
MwoI GCNNNNNNNGC 7 cut(s) 23, 186, 251, 653, 2191, 2795, 2878
NcoI CCATGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 2575
NlaIV GGNNCC 5 cut(s) 12, 678, 1416, 1720, 2173
NmuCI GTSAC 2 cut(s) 813, 941
NruI TCGCGA 1 cut(s) 2784
NsiI ATGCAT 1 cut(s) 688
NspI RCATGY 3 cut(s) 1225, 2440, 2872
PagI TCATGA 2 cut(s) 691, 1600
PciI ACATGT 1 cut(s) 1221
PciSI GCTCTTC 1 cut(s) 2271
PcsI WCGNNNNNNNCGW 1 cut(s) 1252
PctI GAATGC 1 cut(s) 511
PdmI GAANNNNTTC 5 cut(s) 1527, 1665, 1752, 2223, 2832
PflMI CCANNNNNTGG 1 cut(s) 2159
PfoI TCCNGGA 1 cut(s) 1051
PkrI GCNGC 7 cut(s) 52, 253, 322, 1147, 1891, 1894, 2442
PleI GAGTC 4 cut(s) 208, 986, 2001, 2520
PpsI GAGTC 4 cut(s) 208, 986, 2001, 2520
PscI ACATGT 1 cut(s) 1221
PshBI ATTAAT 1 cut(s) 2022
PsiI TTATAA 1 cut(s) 2598
Psp1406I AACGTT 1 cut(s) 61
Psp6I CCWGG 7 cut(s) 189, 763, 1051, 1105, 1425, 1792, 2635
PspFI CCCAGC 2 cut(s) 268, 2159
PspGI CCWGG 7 cut(s) 189, 763, 1051, 1105, 1425, 1792, 2635
PspN4I GGNNCC 5 cut(s) 12, 678, 1416, 1720, 2173
PspOMI GGGCCC 1 cut(s) 1414
PspPI GGNCC 6 cut(s) 677, 1156, 1414, 1415, 1719, 2171
PstI CTGCAG 1 cut(s) 253
PstNI CAGNNNCTG 3 cut(s) 233, 1010, 1895
PsuI RGATCY 5 cut(s) 10, 392, 1291, 1573, 2569
PvuII CAGCTG 5 cut(s) 53, 254, 605, 2159, 2443
RruI TCGCGA 1 cut(s) 2784
RsaI GTAC 9 cut(s) 22, 126, 331, 413, 619, 1285, 1860, 1899, 2088
RsaNI GTAC 9 cut(s) 21, 125, 330, 412, 618, 1284, 1859, 1898, 2087
RseI CAYNNNNRTG 3 cut(s) 696, 1038, 1930
SapI GCTCTTC 1 cut(s) 2271
SaqAI TTAA 5 cut(s) 1067, 1964, 2022, 2294, 2676
SatI GCNGC 7 cut(s) 51, 252, 321, 1146, 1890, 1893, 2441
Sau96I GGNCC 6 cut(s) 677, 1156, 1414, 1415, 1719, 2171
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 4 cut(s) 209, 987, 2002, 2521
ScrFI CCNGG 7 cut(s) 191, 765, 1053, 1107, 1427, 1794, 2637
SduI GDGCHC 3 cut(s) 360, 826, 1418
SfaNI GCATC 8 cut(s) 232, 376, 478, 553, 673, 1030, 1379, 2772
SfcI CTRYAG 3 cut(s) 175, 249, 396
SinI GGWCC 3 cut(s) 677, 1156, 1719
SmiMI CAYNNNNRTG 3 cut(s) 696, 1038, 1930
SmlI CTYRAG 7 cut(s) 128, 1066, 1563, 1586, 2282, 2293, 2790
SmoI CTYRAG 7 cut(s) 128, 1066, 1563, 1586, 2282, 2293, 2790
SpeI ACTAGT 1 cut(s) 1726
SsiI CCGC 3 cut(s) 491, 1145, 1892
SspI AATATT 1 cut(s) 1962
SspMI CTAG 8 cut(s) 18, 383, 858, 1289, 1697, 1727, 2580, 2895
StyD4I CCNGG 7 cut(s) 189, 763, 1051, 1105, 1425, 1792, 2635
StyI CCWWGG 3 cut(s) 442, 2080, 2701
TaaI ACNGT 7 cut(s) 235, 281, 297, 1201, 1283, 1334, 2662
TaiI ACGT 3 cut(s) 64, 586, 1435
TaqI TCGA 5 cut(s) 285, 571, 1669, 2349, 2515
TatI WGTACW 6 cut(s) 20, 124, 411, 617, 1858, 1897
TauI GCSGC 2 cut(s) 1148, 1895
Tru1I TTAA 5 cut(s) 1067, 1964, 2022, 2294, 2676
Tru9I TTAA 5 cut(s) 1067, 1964, 2022, 2294, 2676
TscAI CASTG 5 cut(s) 1663, 1842, 1954, 2375, 2569
TseFI GTSAC 2 cut(s) 813, 941
TseI GCWGC 5 cut(s) 50, 251, 320, 1889, 2440
Tsp45I GTSAC 2 cut(s) 813, 941
TspGWI ACGGA 1 cut(s) 1762
TspRI CASTG 5 cut(s) 1663, 1842, 1954, 2375, 2569
Van91I CCANNNNNTGG 1 cut(s) 2159
Vha464I CTTAAG 2 cut(s) 1066, 2293
VpaK11BI GGWCC 3 cut(s) 677, 1156, 1719
VspI ATTAAT 1 cut(s) 2022
XagI CCTNNNNNAGG 1 cut(s) 2705
XapI RAATTY 9 cut(s) 166, 511, 922, 1126, 1467, 1596, 2066, 2146, 2483
XceI RCATGY 3 cut(s) 1225, 2440, 2872
XcmI CCANNNNNNNNNTGG 1 cut(s) 463
XmnI GAANNNNTTC 5 cut(s) 1527, 1665, 1752, 2223, 2832
XspI CTAG 8 cut(s) 18, 383, 858, 1289, 1697, 1727, 2580, 2895
ZrmI AGTACT 1 cut(s) 22
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.