Prupe.3G190200_v2.0.a1

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0016078
Physical Location & Seq
Forward (+)
20300287 .. 20302117
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G190200.1

Sequence Viewer

Length: 1059 bp
ATGAAGACTGCAGCAAAGAGTTTGGTCATCGAGATCGAAGACGACGACTTCCTTTCTCAGGTGGCGGCGGCTGAGGCCCAGGCGCTCGCCAGCAAGCGTCGGCGTATCAGTATCGCACCCTCTCCAAACTCCGCCGTTTCTTTCCATTCCCGAGCGAATCACAAAGAAGGCTTGGACAGCGAAGATGGCGGTATTTACACCGCGGCTCTCAAAGGAAGCCAAAGAGCAGCCCCCGAGCCTCCACGCGGTGGATTTTCGACGGGGAGAGTCAATGTCGGCCGGACCGGAAACGACGGCATTTCTGCTGGCGGTGGTTCGTGCTTCAAGTGCGGAAAACCGGGACACTGGGCCCGCGATTGTGATGTCACCGGTGGAGGGTATTATGGTAATTCCGGCGGCGGGGACTCTTCGGCTTCGATTTCGGTTCCGGAGAAGAGCTGCCCCTGTGGATTTGGCGCCTGCATGGTGCTCACTGCAAATACTGAGAAAAATCGAGGTCGAAAGTTTTACAAATGCCCTCTTAGACAGGAAAATGGGGGATGCGGTTTCTTTGAGTGGTGTGACAATGCTTCTGGGCCTAATGTAATGGCTAGTACTGGTATGGGTAGTGGTAGCGCTACTAGGACCCATAGTTATGCTGCATCTGATTCTTCATTTCCTGCTCTTGAATGTCCCTGTGGCGGTGGTCTGTGCTTAATCTTAACAGCCAAAACTGGCAAAAACATCGGCAGCCAATTCTATCGTTGCCCTGCAAACGAGGGATGCGGTTTCTTCAAGTGGTGCAATGAGCATACTGGGGCAGCTGCTGGTGTTCTGGGTGGCAGCGCTGCCAAGGTGGAAAGGAAGGTTTTGAGTGAGGCAAGTAACAATGGCTTTGGTGTAAGAACTGGTTCTTCCTGCTTTAAGTGTGGAAAGGAAGGGCATTGGGCAAGAGATTGCCCAGCAGTTCCTTCCTATAATGCTCCAGTTGAGTTGGAAGCACGGTCTGCTGCTTCACCGGGCTCTTGCTATAAGTGTGGCAAGCCAGGGCACTGGGCGAGGGACTGCCCTTCTGGTTAA

Protein Analysis

353

Amino Acids

36.32

Weight (kDa)

8.6

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42860
fragaria_vesca FvH4_6g39844 FvH4_6g39844 FvH4_6g39844
malus_domestica MD17G1125500.v1.1
prunus_persica Prupe.3G190200_v2.0.a1
pyrus_communis pycom17g11770
rosa_chinensis RchiOBHm_Chr2g0154211
rosa_laevigata RLG00000020764
rosa_roxburghii Rroxscaffold_2G00094460
rosa_samantha Rh2AG507900 Rh2BG518900 Rh2CG493200 Rh2DG529800
rosa_wichuraiana Rw2G041710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 455
AccB7I CCANNNNNTGG 1 cut(s) 248
AccII CGCG 3 cut(s) 203, 246, 354
AccIII TCCGGA 1 cut(s) 427
AcoI YGGCCR 1 cut(s) 277
AcyI GRCGYC 1 cut(s) 456
AdeI CACNNNGTG 1 cut(s) 248
AfaI GTAC 1 cut(s) 595
AfeI AGCGCT 2 cut(s) 616, 826
AfiI CCNNNNNNNGG 6 cut(s) 58, 245, 248, 375, 399, 680
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 3 cut(s) 325, 668, 775
AjnI CCWGG 2 cut(s) 78, 1024
AluBI AGCT 2 cut(s) 438, 803
AluI AGCT 2 cut(s) 438, 803
Alw21I GWGCWC 1 cut(s) 471
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 2 cut(s) 150, 233
Aor13HI TCCGGA 1 cut(s) 427
Aor51HI AGCGCT 2 cut(s) 616, 826
AoxI GGCC 4 cut(s) 75, 277, 348, 575
ApaI GGGCCC 1 cut(s) 352
AsiGI ACCGGT 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 889
AspLEI GCGC 4 cut(s) 85, 458, 617, 827
AspS9I GGNCC 6 cut(s) 76, 282, 348, 349, 575, 624
AsuC2I CCSGG 2 cut(s) 339, 999
AsuHPI GGTGA 2 cut(s) 358, 987
AvaI CYCGRG 2 cut(s) 150, 233
AvaII GGWCC 2 cut(s) 282, 624
BaeGI GKGCMC 2 cut(s) 352, 1032
BanI GGYRCC 1 cut(s) 455
BanII GRGCYC 2 cut(s) 352, 1004
BbsI GAAGAC 2 cut(s) 11, 45
Bbv12I GWGCWC 1 cut(s) 471
BbvCI CCTCAGC 1 cut(s) 72
BccI CCATC 1 cut(s) 179
BceAI ACGGC 2 cut(s) 119, 310
BcgI CGANNNNNNTGC 2 cut(s) 706, 740
BciT130I CCWGG 2 cut(s) 80, 1026
BcnI CCSGG 2 cut(s) 339, 999
BfaI CTAG 2 cut(s) 591, 621
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 4 cut(s) 86, 459, 618, 828
BmcAI AGTACT 1 cut(s) 595
Bme1390I CCNGG 4 cut(s) 80, 339, 999, 1026
Bme18I GGWCC 2 cut(s) 282, 624
BmeT110I CYCGRG 2 cut(s) 150, 233
BmgT120I GGNCC 6 cut(s) 76, 282, 348, 349, 575, 624
BmiI GGNNCC 4 cut(s) 350, 426, 457, 626
BmrFI CCNGG 4 cut(s) 80, 339, 999, 1026
BmrI ACTGGG 3 cut(s) 355, 804, 1042
BmsI GCATC 3 cut(s) 530, 650, 752
BmuI ACTGGG 3 cut(s) 355, 804, 1042
BpiI GAAGAC 2 cut(s) 11, 45
BpmI CTGGAG 1 cut(s) 948
Bpu10I CCTNAGC 1 cut(s) 72
BpuMI CCSGG 2 cut(s) 339, 999
BsaHI GRCGYC 1 cut(s) 456
BsaJI CCNNGG 4 cut(s) 78, 201, 831, 1025
BsaWI WCCGGW 3 cut(s) 284, 368, 427
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 245, 248, 375, 399, 680
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 7 cut(s) 350, 601, 718, 799, 892, 965, 1037
Bse3DI GCAATG 1 cut(s) 790
BseAI TCCGGA 1 cut(s) 427
BseBI CCWGG 2 cut(s) 80, 1026
BseDI CCNNGG 4 cut(s) 78, 201, 831, 1025
BseGI GGATG 2 cut(s) 545, 767
BseLI CCNNNNNNNGG 6 cut(s) 58, 245, 248, 375, 399, 680
BseMI GCAATG 1 cut(s) 790
BseMII CTCAG 3 cut(s) 63, 71, 474
BseNI ACTGG 7 cut(s) 350, 601, 718, 799, 892, 965, 1037
BseSI GKGCMC 2 cut(s) 352, 1032
BseX3I CGGCCG 1 cut(s) 277
BseYI CCCAGC 1 cut(s) 940
Bsh1236I CGCG 3 cut(s) 203, 246, 354
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 4 cut(s) 77, 279, 350, 577
BshNI GGYRCC 1 cut(s) 455
BshTI ACCGGT 1 cut(s) 368
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 2 cut(s) 150, 233
BsiSI CCGG 7 cut(s) 280, 285, 338, 369, 393, 428, 998
BslFI GGGAC 4 cut(s) 354, 416, 657, 1055
BslI CCNNNNNNNGG 6 cut(s) 58, 245, 248, 375, 399, 680
BsmFI GGGAC 4 cut(s) 354, 416, 657, 1055
BsnI GGCC 4 cut(s) 77, 279, 350, 577
BsoBI CYCGRG 2 cut(s) 150, 233
Bsp120I GGGCCC 1 cut(s) 348
Bsp1286I GDGCHC 4 cut(s) 352, 471, 1004, 1032
Bsp13I TCCGGA 1 cut(s) 427
Bsp143I GATC 1 cut(s) 33
BspANI GGCC 4 cut(s) 77, 279, 350, 577
BspCNI CTCAG 3 cut(s) 64, 70, 475
BspEI TCCGGA 1 cut(s) 427
BspFNI CGCG 3 cut(s) 203, 246, 354
BspLI GGNNCC 4 cut(s) 350, 426, 457, 626
BspMAI CTGCAG 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 428
BspT107I GGYRCC 1 cut(s) 455
BsrDI GCAATG 1 cut(s) 790
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 7 cut(s) 350, 601, 718, 799, 892, 965, 1037
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 4 cut(s) 78, 201, 831, 1025
BssMI GATC 1 cut(s) 33
BssNI GRCGYC 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 831
Bst2UI CCWGG 2 cut(s) 80, 1026
Bst4CI ACNGT 1 cut(s) 984
Bst6I CTCTTC 2 cut(s) 412, 428
BstACI GRCGYC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 986
BstC8I GCNNGC 7 cut(s) 87, 91, 95, 307, 352, 460, 1022
BstDEI CTNAG 4 cut(s) 57, 72, 483, 521
BstDSI CCRYGG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 56
BstF5I GGATG 2 cut(s) 545, 767
BstFNI CGCG 3 cut(s) 203, 246, 354
BstH2I RGCGCY 4 cut(s) 86, 459, 618, 828
BstHHI GCGC 4 cut(s) 85, 458, 617, 827
BstKTI GATC 1 cut(s) 36
BstMBI GATC 1 cut(s) 33
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 5 cut(s) 74, 177, 186, 327, 986
BstNI CCWGG 2 cut(s) 80, 1026
BstSCI CCNGG 4 cut(s) 78, 337, 997, 1024
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 2 cut(s) 352, 1032
BstUI CGCG 3 cut(s) 203, 246, 354
BstV2I GAAGAC 2 cut(s) 11, 45
BstXI CCANNNNNNTGG 1 cut(s) 1032
BstZI CGGCCG 1 cut(s) 277
BsuRI GGCC 4 cut(s) 77, 279, 350, 577
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 2 cut(s) 545, 767
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 343, 471, 1030
Cac8I GCNNGC 7 cut(s) 87, 91, 95, 307, 352, 460, 1022
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 4 cut(s) 85, 458, 617, 827
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 6 cut(s) 76, 282, 348, 349, 575, 624
Cfr42I CCGCGG 1 cut(s) 204
CpoI CGGWCCG 1 cut(s) 282
CseI GACGC 1 cut(s) 86
Csp6I GTAC 1 cut(s) 594
CspAI ACCGGT 1 cut(s) 368
CspI CGGWCCG 1 cut(s) 282
CviAII CATG 1 cut(s) 463
CviQI GTAC 1 cut(s) 594
DdeI CTNAG 4 cut(s) 57, 72, 483, 521
DinI GGCGCC 1 cut(s) 457
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
DraIII CACNNNGTG 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 277
EagI CGGCCG 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 412, 428
EarI CTCTTC 2 cut(s) 412, 428
EciI GGCGGA 1 cut(s) 121
EclXI CGGCCG 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 831
Eco24I GRGCYC 2 cut(s) 352, 1004
Eco47I GGWCC 2 cut(s) 282, 624
Eco47III AGCGCT 2 cut(s) 616, 826
Eco52I CGGCCG 1 cut(s) 277
Eco88I CYCGRG 2 cut(s) 150, 233
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoO109I RGGNCCY 1 cut(s) 624
EcoRII CCWGG 2 cut(s) 78, 1024
EcoT14I CCWWGG 1 cut(s) 831
EcoT38I GRGCYC 2 cut(s) 352, 1004
EgeI GGCGCC 1 cut(s) 457
EheI GGCGCC 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 831
FaeI CATG 1 cut(s) 466
FaiI YATR 8 cut(s) 384, 464, 602, 630, 636, 792, 957, 1011
FaqI GGGAC 4 cut(s) 354, 416, 657, 1055
FatI CATG 1 cut(s) 462
FauI CCCGC 2 cut(s) 359, 392
FokI GGATG 2 cut(s) 552, 774
FriOI GRGCYC 2 cut(s) 352, 1004
FspBI CTAG 2 cut(s) 591, 621
GlaI GCGC 4 cut(s) 84, 457, 616, 826
GsaI CCCAGC 1 cut(s) 944
GsuI CTGGAG 1 cut(s) 948
HaeII RGCGCY 4 cut(s) 86, 459, 618, 828
HaeIII GGCC 4 cut(s) 77, 279, 350, 577
HapII CCGG 7 cut(s) 280, 285, 338, 369, 393, 428, 998
HgaI GACGC 1 cut(s) 86
HhaI GCGC 4 cut(s) 85, 458, 617, 827
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 1 cut(s) 466
Hin6I GCGC 4 cut(s) 83, 456, 615, 825
HinP1I GCGC 4 cut(s) 83, 456, 615, 825
HinfI GANTC 4 cut(s) 157, 267, 404, 647
HpaII CCGG 7 cut(s) 280, 285, 338, 369, 393, 428, 998
HphI GGTGA 2 cut(s) 358, 987
Hpy188I TCNGA 1 cut(s) 646
Hpy188III TCNNGA 4 cut(s) 31, 150, 428, 665
Hpy99I CGWCG 4 cut(s) 47, 102, 262, 296
HpyAV CCTTC 5 cut(s) 161, 838, 911, 960, 1059
HpyCH4III ACNGT 1 cut(s) 984
HpyCH4V TGCA 6 cut(s) 11, 462, 476, 641, 752, 783
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 177, 186, 327, 986
HpyF3I CTNAG 4 cut(s) 57, 72, 483, 521
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 1 cut(s) 466
HspAI GCGC 4 cut(s) 83, 456, 615, 825
KasI GGCGCC 1 cut(s) 455
Kpn2I TCCGGA 1 cut(s) 427
KspI CCGCGG 1 cut(s) 204
Kzo9I GATC 1 cut(s) 33
LguI GCTCTTC 1 cut(s) 428
LmnI GCTCC 1 cut(s) 967
LweI GCATC 3 cut(s) 530, 650, 752
MaeI CTAG 2 cut(s) 591, 621
MaeIII GTNAC 3 cut(s) 364, 560, 863
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 8 cut(s) 16, 50, 194, 399, 445, 642, 763, 885
MhlI GDGCHC 4 cut(s) 352, 471, 1004, 1032
MluCI AATT 2 cut(s) 388, 734
Mly113I GGCGCC 1 cut(s) 456
MlyI GAGTC 2 cut(s) 276, 398
MmeI TCCRAC 1 cut(s) 954
MnlI CCTC 9 cut(s) 67, 130, 249, 368, 488, 528, 751, 850, 1032
MroI TCCGGA 1 cut(s) 427
MroXI GAANNNNTTC 1 cut(s) 889
MseI TTAA 4 cut(s) 695, 701, 903, 1057
MslI CAYNNNNRTG 1 cut(s) 633
MspA1I CMGCKG 2 cut(s) 203, 803
MspI CCGG 7 cut(s) 280, 285, 338, 369, 393, 428, 998
MspR9I CCNGG 4 cut(s) 80, 339, 999, 1026
MvaI CCWGG 2 cut(s) 80, 1026
MvnI CGCG 3 cut(s) 203, 246, 354
MwoI GCNNNNNNNGC 5 cut(s) 74, 177, 186, 327, 986
NarI GGCGCC 1 cut(s) 456
NciI CCSGG 2 cut(s) 339, 999
NdeII GATC 1 cut(s) 33
NlaIII CATG 1 cut(s) 466
NlaIV GGNNCC 4 cut(s) 350, 426, 457, 626
NmuCI GTSAC 2 cut(s) 364, 560
PciSI GCTCTTC 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 889
PfeI GAWTC 2 cut(s) 157, 647
PflMI CCANNNNNTGG 1 cut(s) 248
PinAI ACCGGT 1 cut(s) 368
PleI GAGTC 2 cut(s) 275, 398
PluTI GGCGCC 1 cut(s) 459
PpsI GAGTC 2 cut(s) 275, 398
PpuMI RGGWCCY 1 cut(s) 624
Psp5II RGGWCCY 1 cut(s) 624
Psp6I CCWGG 2 cut(s) 78, 1024
PspFI CCCAGC 1 cut(s) 940
PspGI CCWGG 2 cut(s) 78, 1024
PspN4I GGNNCC 4 cut(s) 350, 426, 457, 626
PspOMI GGGCCC 1 cut(s) 348
PspPI GGNCC 6 cut(s) 76, 282, 348, 349, 575, 624
PspPPI RGGWCCY 1 cut(s) 624
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 806
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 1 cut(s) 595
RsaNI GTAC 1 cut(s) 594
RseI CAYNNNNRTG 1 cut(s) 633
Rsr2I CGGWCCG 1 cut(s) 282
RsrII CGGWCCG 1 cut(s) 282
SacII CCGCGG 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 428
SaqAI TTAA 4 cut(s) 695, 701, 903, 1057
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 6 cut(s) 76, 282, 348, 349, 575, 624
ScaI AGTACT 1 cut(s) 595
SchI GAGTC 2 cut(s) 276, 398
ScrFI CCNGG 4 cut(s) 80, 339, 999, 1026
SduI GDGCHC 4 cut(s) 352, 471, 1004, 1032
SetI ASST 6 cut(s) 63, 440, 499, 805, 837, 849
SfaNI GCATC 3 cut(s) 530, 650, 752
SfcI CTRYAG 1 cut(s) 9
SfoI GGCGCC 1 cut(s) 457
Sfr303I CCGCGG 1 cut(s) 204
SgrAI CRCCGGYG 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 204
SinI GGWCC 2 cut(s) 282, 624
SmiMI CAYNNNNRTG 1 cut(s) 633
Sse9I AATT 2 cut(s) 388, 734
SspDI GGCGCC 1 cut(s) 455
SspMI CTAG 2 cut(s) 591, 621
StyD4I CCNGG 4 cut(s) 78, 337, 997, 1024
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 1 cut(s) 984
TaqI TCGA 6 cut(s) 30, 36, 257, 416, 493, 499
TasI AATT 2 cut(s) 388, 734
TatI WGTACW 1 cut(s) 593
TauI GCSGC 4 cut(s) 68, 71, 206, 399
TfiI GAWTC 2 cut(s) 157, 647
Tru1I TTAA 4 cut(s) 695, 701, 903, 1057
Tru9I TTAA 4 cut(s) 695, 701, 903, 1057
TscAI CASTG 3 cut(s) 350, 478, 1037
TseFI GTSAC 2 cut(s) 364, 560
Tsp45I GTSAC 2 cut(s) 364, 560
TspDTI ATGAA 2 cut(s) 17, 642
TspRI CASTG 3 cut(s) 350, 478, 1037
Van91I CCANNNNNTGG 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 282, 624
XagI CCTNNNNNAGG 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 889
XspI CTAG 2 cut(s) 591, 621
ZrmI AGTACT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.