RLG00000020764

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016078
Physical Location & Seq
Forward (+)
68543038 .. 68544396
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020764

Sequence Viewer

Length: 1125 bp
ATGAAGACGGAAGCATCCGGATTTCTGATCCAAATCGACGACGACGTAGACGACGACTTCCTTTCTCAGGTTGCCGCCGCGGAGGCCCATGCTCTCGAGAACAAGCGCCGCCGTATCGCCCCCCCCACGCCCTCCCCAAACGCCGTCGCGTCTGCACATCCGCCACCACGCGCAGCGAATCCGAAGGAGAGATCCGACGTCGAAGCCGACGGCGGTCTCTACATCGCGGCGCTCAAAGGAAACCAGAGCGCGTTCTCCGAACCTCCGCGCGGCGGTGGATTTGGCAGAGGGCGAGTCGGGGGGGCCGTAGCGGCGGCGGGAAACGACGGCGTTTTGGGCGGTGATGCGTGCTTCAAGTGCGGGAAATCGGGACACTGGGCTCGCGATTGCGATGCCCCAGGCGGAGGAGGAGGAGGTGGCGGGGGGTATAGTGGTAATTACGGCGGCGGCGGAGGGTATAATGGTAATTACTGCGGCGACGCTGCGTTCGGGATTTCGTTTCCTGAGAAAAGCTGCCCCTGTGGATCTGGAACCTGCGTGGTGCTCACTGCAAATACTGAGAAGAATCGAGGTCGAAAATTCTACAAATGCCCACTTAGACAGGAAAATGGGGGTTGTGGTTTCTTTGAGTGGTGTGATAATGCTTCTGGGCCTAATGCCATGGCTGGTACTGGTAGTGGTAGCTTTAGTAGGACCCAAAACAATGCATCTGATTCTACATTTCCAGCTCTTGAATGTCCCTGTGGTAGTGGGCAATGCCGAATCTTAACAGCTAAAACGGGCAAAAATATCGGCGGCCAATTCTATCGCTGTCCTGGAAATGAGGGAAGCTCTTGCGGTTTCTTCAAGTGGTGCAATGAGAATACTGTGGCCGCTGGTAGTGTTCAAGGTTCACGAATCCCCAAGGTGGTTTATGGTACAAGCGAGACAGGCAGCCAAGGGAGCTATGGTGTTAGGACTGGTTCTTCCTGTTACAAGTGTGGAAAGGAAGGGCATTGGGCAAGAGATTGCTCCGTTCCTTCAAACAATGCTCCGGTTGAATTTGGAGGACGGTCTGCTTTAACAAGCAGCTGCTATAAGTGTGGAAAGACAGGGCACTGGGCCAGGGACTGTCCTTCTGGTTAA

Protein Analysis

375

Amino Acids

38.29

Weight (kDa)

8.28

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 116 - 131 2.9e-07 Zinc knuckle
Zn_ribbon_GRF PF06839 171 - 214 8.3e-16 GRF zinc finger
Zn_ribbon_GRF PF06839 246 - 288 1.7e-14 GRF zinc finger
zf-CCHC PF00098 323 - 338 5.9e-08 Zinc knuckle
zf-CCHC PF00098 357 - 373 9.4e-09 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42860
fragaria_vesca FvH4_6g39844 FvH4_6g39844 FvH4_6g39844
malus_domestica MD17G1125500.v1.1
prunus_persica Prupe.3G190200_v2.0.a1
pyrus_communis pycom17g11770
rosa_chinensis RchiOBHm_Chr2g0154211
rosa_laevigata RLG00000020764
rosa_roxburghii Rroxscaffold_2G00094460
rosa_samantha Rh2AG507900 Rh2BG518900 Rh2CG493200 Rh2DG529800
rosa_wichuraiana Rw2G041710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 542
AccI GTMKAC 1 cut(s) 48
AccII CGCG 8 cut(s) 80, 149, 171, 227, 251, 268, 270, 384
AccIII TCCGGA 1 cut(s) 17
AclWI GGATC 3 cut(s) 22, 186, 532
AcoI YGGCCR 2 cut(s) 796, 870
AcsI RAATTY 2 cut(s) 578, 1040
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 2 cut(s) 670, 919
AfiI CCNNNNNNNGG 5 cut(s) 67, 269, 272, 404, 907
AgsI TTSAA 6 cut(s) 355, 734, 847, 887, 1023, 1040
AjnI CCWGG 3 cut(s) 397, 814, 1103
AluBI AGCT 7 cut(s) 513, 684, 728, 773, 831, 945, 1071
AluI AGCT 7 cut(s) 513, 684, 728, 773, 831, 945, 1071
Alw21I GWGCWC 1 cut(s) 546
Alw26I GTCTC 2 cut(s) 221, 920
AlwI GGATC 3 cut(s) 22, 186, 532
AlwNI CAGNNNCTG 1 cut(s) 1110
Ama87I CYCGRG 1 cut(s) 95
Aor13HI TCCGGA 1 cut(s) 17
AoxI GGCC 6 cut(s) 84, 303, 650, 796, 870, 1101
ApeKI GCWGC 6 cut(s) 173, 482, 513, 933, 1068, 1071
ApoI RAATTY 2 cut(s) 578, 1040
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AspLEI GCGC 5 cut(s) 108, 173, 232, 251, 270
AspS9I GGNCC 5 cut(s) 85, 303, 650, 693, 1101
AsuHPI GGTGA 1 cut(s) 353
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 1 cut(s) 693
BaeGI GKGCMC 1 cut(s) 1098
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 546
BbvI GCAGC 6 cut(s) 185, 469, 500, 945, 1058, 1080
BceAI ACGGC 6 cut(s) 96, 128, 226, 290, 343, 457
BcgI CGANNNNNNTGC 4 cut(s) 374, 408, 772, 806
BciT130I CCWGG 3 cut(s) 399, 816, 1105
BcoDI GTCTC 2 cut(s) 221, 920
BfoI RGCGCY 2 cut(s) 109, 233
BfuAI ACCTGC 1 cut(s) 542
BglI GCCNNNNNGGC 2 cut(s) 83, 311
Bme1390I CCNGG 3 cut(s) 399, 816, 1105
Bme18I GGWCC 1 cut(s) 693
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 5 cut(s) 85, 303, 650, 693, 1101
BmiI GGNNCC 3 cut(s) 304, 532, 695
BmrFI CCNGG 3 cut(s) 399, 816, 1105
BmrI ACTGGG 2 cut(s) 385, 1108
BmsI GCATC 4 cut(s) 23, 334, 382, 716
BmuI ACTGGG 2 cut(s) 385, 1108
BoxI GACNNNNGTC 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BsaBI GATNNNNATC 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 198
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 6 cut(s) 78, 397, 660, 903, 937, 1104
BsaWI WCCGGW 2 cut(s) 17, 1033
BsaXI ACNNNNNCTCC 2 cut(s) 934, 964
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 269, 272, 404, 907
Bse1I ACTGG 4 cut(s) 380, 676, 964, 1103
Bse3DI GCAATG 2 cut(s) 761, 862
Bse8I GATNNNNATC 1 cut(s) 32
BseAI TCCGGA 1 cut(s) 17
BseBI CCWGG 3 cut(s) 399, 816, 1105
BseDI CCNNGG 6 cut(s) 78, 397, 660, 903, 937, 1104
BseGI GGATG 2 cut(s) 14, 157
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 5 cut(s) 67, 269, 272, 404, 907
BseMI GCAATG 2 cut(s) 761, 862
BseMII CTCAG 3 cut(s) 80, 495, 549
BseNI ACTGG 4 cut(s) 380, 676, 964, 1103
BseRI GAGGAG 3 cut(s) 420, 423, 426
BseSI GKGCMC 1 cut(s) 1098
BseXI GCAGC 6 cut(s) 185, 469, 500, 945, 1058, 1080
BsgI GTGCAG 1 cut(s) 138
Bsh1236I CGCG 8 cut(s) 80, 149, 171, 227, 251, 268, 270, 384
BshFI GGCC 6 cut(s) 86, 305, 652, 798, 872, 1103
BsiHKAI GWGCWC 1 cut(s) 546
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 18, 1034
BslFI GGGAC 3 cut(s) 384, 723, 1121
BslI CCNNNNNNNGG 5 cut(s) 67, 269, 272, 404, 907
BsmAI GTCTC 2 cut(s) 221, 920
BsmFI GGGAC 3 cut(s) 384, 723, 1121
BsnI GGCC 6 cut(s) 86, 305, 652, 798, 872, 1103
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 3 cut(s) 382, 546, 1098
Bsp13I TCCGGA 1 cut(s) 17
Bsp143I GATC 3 cut(s) 27, 191, 524
Bsp19I CCATGG 1 cut(s) 660
Bsp68I TCGCGA 1 cut(s) 384
BspANI GGCC 6 cut(s) 86, 305, 652, 798, 872, 1103
BspCNI CTCAG 3 cut(s) 79, 496, 550
BspEI TCCGGA 1 cut(s) 17
BspFNI CGCG 8 cut(s) 80, 149, 171, 227, 251, 268, 270, 384
BspLI GGNNCC 3 cut(s) 304, 532, 695
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 3 cut(s) 22, 186, 532
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 2 cut(s) 761, 862
BsrI ACTGG 4 cut(s) 380, 676, 964, 1103
BssECI CCNNGG 6 cut(s) 78, 397, 660, 903, 937, 1104
BssMI GATC 3 cut(s) 27, 191, 524
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 3 cut(s) 660, 903, 937
Bst2UI CCWGG 3 cut(s) 399, 816, 1105
Bst4CI ACNGT 3 cut(s) 868, 1053, 1112
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 349, 382
BstDEI CTNAG 4 cut(s) 66, 504, 558, 596
BstDSI CCRYGG 2 cut(s) 78, 660
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 14, 157
BstFNI CGCG 8 cut(s) 80, 149, 171, 227, 251, 268, 270, 384
BstH2I RGCGCY 2 cut(s) 109, 233
BstHHI GCGC 5 cut(s) 108, 173, 232, 251, 270
BstKTI GATC 3 cut(s) 30, 194, 527
BstMAI GTCTC 2 cut(s) 221, 920
BstMBI GATC 3 cut(s) 27, 191, 524
BstMWI GCNNNNNNNGC 6 cut(s) 83, 311, 336, 357, 930, 942
BstNI CCWGG 3 cut(s) 399, 816, 1105
BstPAI GACNNNNGTC 1 cut(s) 213
BstSCI CCNGG 3 cut(s) 397, 814, 1103
BstSLI GKGCMC 1 cut(s) 1098
BstUI CGCG 8 cut(s) 80, 149, 171, 227, 251, 268, 270, 384
BstV1I GCAGC 6 cut(s) 185, 469, 500, 945, 1058, 1080
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 191, 524
BstYI RGATCY 2 cut(s) 191, 524
BsuRI GGCC 6 cut(s) 86, 305, 652, 798, 872, 1103
BtgI CCRYGG 2 cut(s) 78, 660
BtgZI GCGATG 2 cut(s) 208, 405
BtsCI GGATG 2 cut(s) 14, 157
BtsI GCAGTG 1 cut(s) 546
BtsIMutI CAGTG 3 cut(s) 373, 546, 1096
BtuMI TCGCGA 1 cut(s) 384
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 2 cut(s) 349, 382
CaiI CAGNNNCTG 1 cut(s) 1110
CfoI GCGC 5 cut(s) 108, 173, 232, 251, 270
Cfr13I GGNCC 5 cut(s) 85, 303, 650, 693, 1101
Cfr42I CCGCGG 1 cut(s) 81
CseI GACGC 2 cut(s) 138, 488
Csp6I GTAC 2 cut(s) 669, 918
CviAII CATG 2 cut(s) 89, 661
CviQI GTAC 2 cut(s) 669, 918
DdeI CTNAG 4 cut(s) 66, 504, 558, 596
DpnI GATC 3 cut(s) 29, 193, 526
DpnII GATC 3 cut(s) 27, 191, 524
EaeI YGGCCR 2 cut(s) 796, 870
EciI GGCGGA 3 cut(s) 150, 417, 465
Eco130I CCWWGG 3 cut(s) 660, 903, 937
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 693
Eco88I CYCGRG 1 cut(s) 95
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 693
EcoRII CCWGG 3 cut(s) 397, 814, 1103
EcoT14I CCWWGG 3 cut(s) 660, 903, 937
EcoT22I ATGCAT 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 3 cut(s) 660, 903, 937
FaeI CATG 2 cut(s) 92, 664
FaiI YATR 7 cut(s) 90, 429, 459, 662, 915, 948, 1077
FaqI GGGAC 3 cut(s) 384, 723, 1121
FatI CATG 2 cut(s) 88, 660
FauI CCCGC 3 cut(s) 310, 353, 413
FblI GTMKAC 1 cut(s) 48
FokI GGATG 1 cut(s) 144
FriOI GRGCYC 1 cut(s) 382
GlaI GCGC 5 cut(s) 107, 172, 231, 250, 269
HaeII RGCGCY 2 cut(s) 109, 233
HaeIII GGCC 6 cut(s) 86, 305, 652, 798, 872, 1103
HapII CCGG 2 cut(s) 18, 1034
HgaI GACGC 2 cut(s) 138, 488
HhaI GCGC 5 cut(s) 108, 173, 232, 251, 270
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 92, 664
Hin6I GCGC 5 cut(s) 106, 171, 230, 249, 268
HinP1I GCGC 5 cut(s) 106, 171, 230, 249, 268
HinfI GANTC 6 cut(s) 178, 294, 565, 713, 762, 897
HpaII CCGG 2 cut(s) 18, 1034
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 49, 893
Hpy188I TCNGA 5 cut(s) 27, 183, 196, 259, 712
Hpy8I GTNNAC 2 cut(s) 49, 893
HpyAV CCTTC 4 cut(s) 178, 983, 1029, 1125
HpyCH4III ACNGT 3 cut(s) 868, 1053, 1112
HpyCH4IV ACGT 2 cut(s) 45, 198
HpyCH4V TGCA 4 cut(s) 155, 551, 707, 855
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 311, 336, 357, 930, 942
HpyF3I CTNAG 4 cut(s) 66, 504, 558, 596
HpySE526I ACGT 2 cut(s) 45, 198
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 92, 664
HspAI GCGC 5 cut(s) 106, 171, 230, 249, 268
Kpn2I TCCGGA 1 cut(s) 17
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 3 cut(s) 27, 191, 524
LmnI GCTCC 3 cut(s) 942, 1016, 1036
Lsp1109I GCAGC 6 cut(s) 185, 469, 500, 945, 1058, 1080
LweI GCATC 4 cut(s) 23, 334, 382, 716
MaeII ACGT 2 cut(s) 45, 198
MaeIII GTNAC 1 cut(s) 971
MalI GATC 3 cut(s) 29, 193, 526
MboI GATC 3 cut(s) 27, 191, 524
MboII GAAGA 4 cut(s) 16, 574, 835, 957
MflI RGATCY 2 cut(s) 191, 524
MhlI GDGCHC 3 cut(s) 382, 546, 1098
MluCI AATT 5 cut(s) 436, 466, 578, 800, 1040
MlyI GAGTC 1 cut(s) 303
MmeI TCCRAC 1 cut(s) 219
Mph1103I ATGCAT 1 cut(s) 709
MroI TCCGGA 1 cut(s) 17
MseI TTAA 3 cut(s) 767, 1061, 1123
MspA1I CMGCKG 3 cut(s) 80, 875, 1071
MspI CCGG 2 cut(s) 18, 1034
MspR9I CCNGG 3 cut(s) 399, 816, 1105
MvaI CCWGG 3 cut(s) 399, 816, 1105
MvnI CGCG 8 cut(s) 80, 149, 171, 227, 251, 268, 270, 384
MwoI GCNNNNNNNGC 6 cut(s) 83, 311, 336, 357, 930, 942
NcoI CCATGG 1 cut(s) 660
NdeII GATC 3 cut(s) 27, 191, 524
NlaIII CATG 2 cut(s) 92, 664
NlaIV GGNNCC 3 cut(s) 304, 532, 695
NruI TCGCGA 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 709
PaeR7I CTCGAG 1 cut(s) 95
PcsI WCGNNNNNNNCGW 5 cut(s) 42, 48, 51, 204, 303
PfeI GAWTC 5 cut(s) 178, 565, 713, 762, 897
PfoI TCCNGGA 1 cut(s) 814
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 693
PshAI GACNNNNGTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 693
Psp6I CCWGG 3 cut(s) 397, 814, 1103
PspGI CCWGG 3 cut(s) 397, 814, 1103
PspN4I GGNNCC 3 cut(s) 304, 532, 695
PspPI GGNCC 5 cut(s) 85, 303, 650, 693, 1101
PspPPI RGGWCCY 1 cut(s) 693
PstNI CAGNNNCTG 1 cut(s) 1110
PsuI RGATCY 2 cut(s) 191, 524
PvuII CAGCTG 1 cut(s) 1071
RruI TCGCGA 1 cut(s) 384
RsaI GTAC 2 cut(s) 670, 919
RsaNI GTAC 2 cut(s) 669, 918
SacII CCGCGG 1 cut(s) 81
SaqAI TTAA 3 cut(s) 767, 1061, 1123
Sau3AI GATC 3 cut(s) 27, 191, 524
Sau96I GGNCC 5 cut(s) 85, 303, 650, 693, 1101
SchI GAGTC 1 cut(s) 303
ScrFI CCNGG 3 cut(s) 399, 816, 1105
SduI GDGCHC 3 cut(s) 382, 546, 1098
SfaNI GCATC 4 cut(s) 23, 334, 382, 716
Sfr274I CTCGAG 1 cut(s) 95
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SinI GGWCC 1 cut(s) 693
SlaI CTCGAG 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 5 cut(s) 436, 466, 578, 800, 1040
StyD4I CCNGG 3 cut(s) 397, 814, 1103
StyI CCWWGG 3 cut(s) 660, 903, 937
TaaI ACNGT 3 cut(s) 868, 1053, 1112
TaiI ACGT 2 cut(s) 48, 201
TaqI TCGA 5 cut(s) 36, 96, 201, 568, 574
TasI AATT 5 cut(s) 436, 466, 578, 800, 1040
TfiI GAWTC 5 cut(s) 178, 565, 713, 762, 897
Tru1I TTAA 3 cut(s) 767, 1061, 1123
Tru9I TTAA 3 cut(s) 767, 1061, 1123
TscAI CASTG 3 cut(s) 380, 553, 1103
TseI GCWGC 6 cut(s) 173, 482, 513, 933, 1068, 1071
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 23, 1003
TspRI CASTG 3 cut(s) 380, 553, 1103
VpaK11BI GGWCC 1 cut(s) 693
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 2 cut(s) 578, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 48
ZraI GACGTC 1 cut(s) 199
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.