Rh2BG518900

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016078
Physical Location & Seq
Forward (+)
72794974 .. 72796329
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG518900.1

Sequence Viewer

Length: 1122 bp
ATGAAGACGGAAGCACCCGGATTTCTGATCCAAATCGACGACGACGTAGACGACGACTTCCTTTCTCAGGTTGCCGCCGCCGAGGCCCATGCTCTCGCGAACAAGCGCCGCCGTATCGCCACTCCCACGCCCTCCCCAAACGCCGTCGCGTCGGCACCTCCGCCACCAAGCCCAGCGAATCCGAAGGAGAGATCAGACTCCGAAGCCGACGGCGGTCTCTACATCGCGGCGCTCAAAGGAAACCAGAGCGCGTTCTCCGAGCCTCCGCGCGGCGGTGGATTTGGCAGAGGGCGAGTCGGCGGGGCCGTAGCGGCCTCGGGAAACGACGGCGTTTTGGGCGGTGATGCGTGCTTCAAGTGCGGGAAATCGGGACACTGGGCTCGCGATTGCGATGCCCCAGGCGGAGGAAGAGGAGGAGGCGGGGGGTATAGTGGTAATTACGGCGGCGGAGGGTATAATGGTAATTACGGCGGCGACGCTGCGTCCGCCATTTTGTTTCCTGAGAAAAGCTGCCCCTGTGGATCTGGAACCTGCGTGGTGCTCACTGCAAATACTGAGAAGAATCGAGGTCGAAAATTCTACAAATGCCCACTTAGACAGGAAAATGGGGGTTGTGGTTTCTTTGAGTGGTGTGATAATGCTTCTGGGCCTAATGCCATGGCTGGTGCTGGTAGTGGTAGCTTTAGTAGGACCCAAAACTATGCATCTGATTCTACATTTCCAGCTCTTGAATGTCCCTGTGGTGGTGGGCAATGCCGAATCTTAACAGCTAAAACGGGCAAAAATATCGGCAGCCAATTCTATCGCTGTCCTGGAAATGAGGGAAGCTCTTGCGGTTTCTTCAAGTGGTGCAGTGAGAATACTGTGGCAGCTGGTAGTGTTCAGGGTGCACGAATCCCCAAGGTGGTTTATGGTTCAAGCGAGACAGGCAGCCAAGGGAGCTATGGTGTTAGGACTGGTTCTTCCTGTTACAAGTGTGGAAAGGAAGGGCATTGGGCAAGAGATTGCTCCGTTCCTTCAAACAATGCTCCGGTTGAATTTGGAGGACGGTCTGCTTCAACAAGCAGCTGCTATAAGTGTGGAAAGACGGGGCACTGGGCCAGGGACTGTCCTTCTGGTTAA

Protein Analysis

373

Amino Acids

38.08

Weight (kDa)

8.41

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 116 - 131 2.9e-07 Zinc knuckle
Zn_ribbon_GRF PF06839 170 - 213 8.2e-16 GRF zinc finger
Zn_ribbon_GRF PF06839 245 - 286 1.9e-14 GRF zinc finger
zf-CCHC PF00098 322 - 337 5.9e-08 Zinc knuckle
zf-CCHC PF00098 356 - 372 9.3e-09 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42860
fragaria_vesca FvH4_6g39844 FvH4_6g39844 FvH4_6g39844
malus_domestica MD17G1125500.v1.1
prunus_persica Prupe.3G190200_v2.0.a1
pyrus_communis pycom17g11770
rosa_chinensis RchiOBHm_Chr2g0154211
rosa_laevigata RLG00000020764
rosa_roxburghii Rroxscaffold_2G00094460
rosa_samantha Rh2AG507900 Rh2BG518900 Rh2CG493200 Rh2DG529800
rosa_wichuraiana Rw2G041710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 539
AccB1I GGYRCC 1 cut(s) 154
AccI GTMKAC 1 cut(s) 48
AccII CGCG 7 cut(s) 98, 149, 227, 251, 268, 270, 384
AclWI GGATC 2 cut(s) 22, 529
AcsI RAATTY 2 cut(s) 575, 1037
AfiI CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 743, 904
AgsI TTSAA 7 cut(s) 355, 731, 844, 918, 1020, 1037, 1059
AhdI GACNNNNNGTC 1 cut(s) 481
AjnI CCWGG 3 cut(s) 397, 811, 1100
AluBI AGCT 8 cut(s) 510, 681, 725, 770, 828, 872, 942, 1068
AluI AGCT 8 cut(s) 510, 681, 725, 770, 828, 872, 942, 1068
Alw21I GWGCWC 2 cut(s) 543, 892
Alw26I GTCTC 2 cut(s) 221, 917
Alw44I GTGCAC 1 cut(s) 888
AlwI GGATC 2 cut(s) 22, 529
AlwNI CAGNNNCTG 1 cut(s) 1107
Ama87I CYCGRG 1 cut(s) 316
AoxI GGCC 5 cut(s) 84, 303, 312, 647, 1098
ApaLI GTGCAC 1 cut(s) 888
ApeKI GCWGC 7 cut(s) 479, 510, 792, 869, 930, 1065, 1068
ApoI RAATTY 2 cut(s) 575, 1037
AspLEI GCGC 4 cut(s) 108, 232, 251, 270
AspS9I GGNCC 5 cut(s) 85, 303, 647, 690, 1098
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 353
AvaI CYCGRG 1 cut(s) 316
AvaII GGWCC 1 cut(s) 690
BaeGI GKGCMC 2 cut(s) 892, 1095
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 543, 892
BbvI GCAGC 7 cut(s) 466, 497, 804, 881, 942, 1055, 1077
BceAI ACGGC 7 cut(s) 96, 128, 226, 290, 343, 457, 484
BcgI CGANNNNNNTGC 6 cut(s) 71, 105, 374, 408, 769, 803
BciT130I CCWGG 3 cut(s) 399, 813, 1102
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 2 cut(s) 221, 917
BfoI RGCGCY 2 cut(s) 109, 233
BfuAI ACCTGC 1 cut(s) 539
BglI GCCNNNNNGGC 2 cut(s) 83, 311
Bme1390I CCNGG 4 cut(s) 18, 399, 813, 1102
Bme18I GGWCC 1 cut(s) 690
BmeRI GACNNNNNGTC 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 316
BmgT120I GGNCC 5 cut(s) 85, 303, 647, 690, 1098
BmiI GGNNCC 4 cut(s) 156, 304, 529, 692
BmrFI CCNGG 4 cut(s) 18, 399, 813, 1102
BmrI ACTGGG 2 cut(s) 385, 1105
BmsI GCATC 3 cut(s) 334, 382, 713
BmuI ACTGGG 2 cut(s) 385, 1105
BoxI GACNNNNGTC 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpuMI CCSGG 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 7 cut(s) 81, 315, 397, 657, 900, 934, 1101
BsaWI WCCGGW 1 cut(s) 1030
BsaXI ACNNNNNCTCC 2 cut(s) 931, 961
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 743, 904
Bse1I ACTGG 3 cut(s) 380, 961, 1100
Bse3DI GCAATG 1 cut(s) 758
Bse8I GATNNNNATC 1 cut(s) 32
BseBI CCWGG 3 cut(s) 399, 813, 1102
BseDI CCNNGG 7 cut(s) 81, 315, 397, 657, 900, 934, 1101
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 743, 904
BseMI GCAATG 1 cut(s) 758
BseMII CTCAG 3 cut(s) 80, 492, 546
BseNI ACTGG 3 cut(s) 380, 961, 1100
BseRI GAGGAG 2 cut(s) 426, 429
BseSI GKGCMC 2 cut(s) 892, 1095
BseXI GCAGC 7 cut(s) 466, 497, 804, 881, 942, 1055, 1077
BseYI CCCAGC 1 cut(s) 172
BsgI GTGCAG 1 cut(s) 871
Bsh1236I CGCG 7 cut(s) 98, 149, 227, 251, 268, 270, 384
BshFI GGCC 5 cut(s) 86, 305, 314, 649, 1100
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 543, 892
BsiHKCI CYCGRG 1 cut(s) 316
BsiSI CCGG 2 cut(s) 18, 1031
BslFI GGGAC 3 cut(s) 384, 720, 1118
BslI CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 743, 904
BsmAI GTCTC 2 cut(s) 221, 917
BsmFI GGGAC 3 cut(s) 384, 720, 1118
BsnI GGCC 5 cut(s) 86, 305, 314, 649, 1100
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 316
Bsp1286I GDGCHC 4 cut(s) 382, 543, 892, 1095
Bsp143I GATC 3 cut(s) 27, 191, 521
Bsp19I CCATGG 1 cut(s) 657
Bsp68I TCGCGA 2 cut(s) 98, 384
BspANI GGCC 5 cut(s) 86, 305, 314, 649, 1100
BspCNI CTCAG 3 cut(s) 79, 493, 547
BspFNI CGCG 7 cut(s) 98, 149, 227, 251, 268, 270, 384
BspLI GGNNCC 4 cut(s) 156, 304, 529, 692
BspMI ACCTGC 1 cut(s) 539
BspPI GGATC 2 cut(s) 22, 529
BspT107I GGYRCC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 758
BsrI ACTGG 3 cut(s) 380, 961, 1100
BssECI CCNNGG 7 cut(s) 81, 315, 397, 657, 900, 934, 1101
BssMI GATC 3 cut(s) 27, 191, 521
BssT1I CCWWGG 3 cut(s) 657, 900, 934
Bst2UI CCWGG 3 cut(s) 399, 813, 1102
Bst4CI ACNGT 3 cut(s) 865, 1050, 1109
Bst6I CTCTTC 1 cut(s) 403
BstC8I GCNNGC 2 cut(s) 349, 382
BstDEI CTNAG 4 cut(s) 66, 501, 555, 593
BstDSI CCRYGG 1 cut(s) 657
BstENI CCTNNNNNAGG 1 cut(s) 65
BstFNI CGCG 7 cut(s) 98, 149, 227, 251, 268, 270, 384
BstH2I RGCGCY 2 cut(s) 109, 233
BstHHI GCGC 4 cut(s) 108, 232, 251, 270
BstKTI GATC 3 cut(s) 30, 194, 524
BstMAI GTCTC 2 cut(s) 221, 917
BstMBI GATC 3 cut(s) 27, 191, 521
BstMWI GCNNNNNNNGC 7 cut(s) 83, 311, 336, 357, 485, 927, 939
BstNI CCWGG 3 cut(s) 399, 813, 1102
BstPAI GACNNNNGTC 1 cut(s) 213
BstSCI CCNGG 4 cut(s) 16, 397, 811, 1100
BstSLI GKGCMC 2 cut(s) 892, 1095
BstUI CGCG 7 cut(s) 98, 149, 227, 251, 268, 270, 384
BstV1I GCAGC 7 cut(s) 466, 497, 804, 881, 942, 1055, 1077
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 521
BstYI RGATCY 1 cut(s) 521
BsuRI GGCC 5 cut(s) 86, 305, 314, 649, 1100
BtgI CCRYGG 1 cut(s) 657
BtgZI GCGATG 2 cut(s) 208, 405
BtsI GCAGTG 2 cut(s) 543, 859
BtsIMutI CAGTG 4 cut(s) 373, 543, 859, 1093
BtuMI TCGCGA 2 cut(s) 98, 384
BveI ACCTGC 1 cut(s) 539
Cac8I GCNNGC 2 cut(s) 349, 382
CaiI CAGNNNCTG 1 cut(s) 1107
CfoI GCGC 4 cut(s) 108, 232, 251, 270
Cfr13I GGNCC 5 cut(s) 85, 303, 647, 690, 1098
CseI GACGC 3 cut(s) 138, 471, 485
CviAII CATG 2 cut(s) 89, 658
DdeI CTNAG 4 cut(s) 66, 501, 555, 593
DpnI GATC 3 cut(s) 29, 193, 523
DpnII GATC 3 cut(s) 27, 191, 521
DriI GACNNNNNGTC 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 403
Eam1105I GACNNNNNGTC 1 cut(s) 481
EarI CTCTTC 1 cut(s) 403
EciI GGCGGA 4 cut(s) 150, 417, 462, 475
Eco130I CCWWGG 3 cut(s) 657, 900, 934
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 690
EcoRII CCWGG 3 cut(s) 397, 811, 1100
EcoT14I CCWWGG 3 cut(s) 657, 900, 934
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 3 cut(s) 657, 900, 934
FaeI CATG 2 cut(s) 92, 661
FaiI YATR 8 cut(s) 90, 429, 456, 659, 702, 912, 945, 1074
FaqI GGGAC 3 cut(s) 384, 720, 1118
FatI CATG 2 cut(s) 88, 657
FauI CCCGC 3 cut(s) 293, 353, 413
FblI GTMKAC 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 382
GlaI GCGC 4 cut(s) 107, 231, 250, 269
GsaI CCCAGC 1 cut(s) 176
HaeII RGCGCY 2 cut(s) 109, 233
HaeIII GGCC 5 cut(s) 86, 305, 314, 649, 1100
HapII CCGG 2 cut(s) 18, 1031
HgaI GACGC 3 cut(s) 138, 471, 485
HhaI GCGC 4 cut(s) 108, 232, 251, 270
Hin1II CATG 2 cut(s) 92, 661
Hin6I GCGC 4 cut(s) 106, 230, 249, 268
HinP1I GCGC 4 cut(s) 106, 230, 249, 268
HinfI GANTC 7 cut(s) 178, 197, 294, 562, 710, 759, 894
HpaII CCGG 2 cut(s) 18, 1031
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 2 cut(s) 49, 890
Hpy188I TCNGA 6 cut(s) 27, 183, 196, 202, 259, 709
Hpy188III TCNNGA 7 cut(s) 97, 318, 369, 383, 500, 525, 728
Hpy8I GTNNAC 2 cut(s) 49, 890
Hpy99I CGWCG 9 cut(s) 41, 44, 47, 56, 149, 154, 212, 329, 479
HpyAV CCTTC 4 cut(s) 178, 980, 1026, 1122
HpyCH4III ACNGT 3 cut(s) 865, 1050, 1109
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 4 cut(s) 548, 704, 852, 890
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 311, 336, 357, 485, 927, 939
HpyF3I CTNAG 4 cut(s) 66, 501, 555, 593
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 2 cut(s) 92, 661
HspAI GCGC 4 cut(s) 106, 230, 249, 268
Kzo9I GATC 3 cut(s) 27, 191, 521
LmnI GCTCC 3 cut(s) 939, 1013, 1033
Lsp1109I GCAGC 7 cut(s) 466, 497, 804, 881, 942, 1055, 1077
LweI GCATC 3 cut(s) 334, 382, 713
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 968
MalI GATC 3 cut(s) 29, 193, 523
MboI GATC 3 cut(s) 27, 191, 521
MboII GAAGA 5 cut(s) 16, 420, 571, 832, 954
MflI RGATCY 1 cut(s) 521
MhlI GDGCHC 4 cut(s) 382, 543, 892, 1095
MluCI AATT 5 cut(s) 436, 463, 575, 797, 1037
MlyI GAGTC 2 cut(s) 191, 303
Mph1103I ATGCAT 1 cut(s) 706
MseI TTAA 2 cut(s) 764, 1120
MspA1I CMGCKG 2 cut(s) 872, 1068
MspI CCGG 2 cut(s) 18, 1031
MspR9I CCNGG 4 cut(s) 18, 399, 813, 1102
MvaI CCWGG 3 cut(s) 399, 813, 1102
MvnI CGCG 7 cut(s) 98, 149, 227, 251, 268, 270, 384
MwoI GCNNNNNNNGC 7 cut(s) 83, 311, 336, 357, 485, 927, 939
NciI CCSGG 1 cut(s) 18
NcoI CCATGG 1 cut(s) 657
NdeII GATC 3 cut(s) 27, 191, 521
NlaIII CATG 2 cut(s) 92, 661
NlaIV GGNNCC 4 cut(s) 156, 304, 529, 692
NmeAIII GCCGAG 1 cut(s) 106
NruI TCGCGA 2 cut(s) 98, 384
NsiI ATGCAT 1 cut(s) 706
PcsI WCGNNNNNNNCGW 4 cut(s) 42, 48, 51, 303
PfeI GAWTC 5 cut(s) 178, 562, 710, 759, 894
PfoI TCCNGGA 1 cut(s) 811
PleI GAGTC 2 cut(s) 191, 302
PpsI GAGTC 2 cut(s) 191, 302
PpuMI RGGWCCY 1 cut(s) 690
PshAI GACNNNNGTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 690
Psp6I CCWGG 3 cut(s) 397, 811, 1100
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 3 cut(s) 397, 811, 1100
PspN4I GGNNCC 4 cut(s) 156, 304, 529, 692
PspPI GGNCC 5 cut(s) 85, 303, 647, 690, 1098
PspPPI RGGWCCY 1 cut(s) 690
PstNI CAGNNNCTG 1 cut(s) 1107
PsuI RGATCY 1 cut(s) 521
PvuII CAGCTG 2 cut(s) 872, 1068
RruI TCGCGA 2 cut(s) 98, 384
SaqAI TTAA 2 cut(s) 764, 1120
Sau3AI GATC 3 cut(s) 27, 191, 521
Sau96I GGNCC 5 cut(s) 85, 303, 647, 690, 1098
SchI GAGTC 2 cut(s) 191, 303
ScrFI CCNGG 4 cut(s) 18, 399, 813, 1102
SduI GDGCHC 4 cut(s) 382, 543, 892, 1095
SfaNI GCATC 3 cut(s) 334, 382, 713
SfiI GGCCNNNNNGGCC 1 cut(s) 311
SinI GGWCC 1 cut(s) 690
Sse9I AATT 5 cut(s) 436, 463, 575, 797, 1037
StyD4I CCNGG 4 cut(s) 16, 397, 811, 1100
StyI CCWWGG 3 cut(s) 657, 900, 934
TaaI ACNGT 3 cut(s) 865, 1050, 1109
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 36, 565, 571
TasI AATT 5 cut(s) 436, 463, 575, 797, 1037
TauI GCSGC 8 cut(s) 77, 80, 111, 230, 273, 314, 447, 474
TfiI GAWTC 5 cut(s) 178, 562, 710, 759, 894
Tru1I TTAA 2 cut(s) 764, 1120
Tru9I TTAA 2 cut(s) 764, 1120
TscAI CASTG 4 cut(s) 380, 550, 859, 1100
TseI GCWGC 7 cut(s) 479, 510, 792, 869, 930, 1065, 1068
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 23, 1000
TspRI CASTG 4 cut(s) 380, 550, 859, 1100
VneI GTGCAC 1 cut(s) 888
VpaK11BI GGWCC 1 cut(s) 690
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 2 cut(s) 575, 1037
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XmiI GTMKAC 1 cut(s) 48
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.