Rh2CG493200

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016078
Physical Location & Seq
Forward (+)
66194369 .. 66195718
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG493200.1

Sequence Viewer

Length: 1116 bp
ATGAAGACGGAAGCACCCGGATTTCTGATCCAAATAGACGACGACGTAGACGACGACTTCCTTTCTCAGGTTGCCGCCGTCGAGGCCCATGCTCTCGCGAACAAGCGCCGCCGTATCGCCACCCCCACGCCCTCCCCAAAAGCCGTCGCGTCGGCACCTCCGCCACCACGCGCAGCGAATTTGAAGGAGAGATCCGACTCCGAAGCCGACGGCGGTCTCTACATCGCGGCGCTCAAAGGAAACCAGAGCGCGTTCTCCGAGCCTCCGCGCGGCGGTGGATTTGGCAGAGGGCGAGTCGGCGGGGCCGTAGCGGCGGCGGGAAACGACGGCGTTTTGGGCGGTGATGCGTGCTTCAAGTGCGGGAAACCGGGACACTGGGCTCGCGATTGCGATGCCCCAAGCGGAGGAGGCGGCGGAGGGTATAGTGGTAATTACGGCGGCGGAGGGTATAATGGTAATTACGGCGGCGACGCTGCGTCCGCGATTTCGTTTCCTGAGAAAAGCTGCCCCTGTGGATCTGGAACCTGCGTGGTGCTCACCGCAAATACTGAGAAGAATCGAGGTCGAAAATTCTACAAATGCCCACTTAGACAGGAAAATGGGGGTTGTGGTTTCTTTGAGTGGTGTGATAATGCTTCTGGGCCTAATGCCATGGCTGGTGCTGGTAGTGGTAGCTTTAGTAGGACCCAAAACTATGCATCTGATTCTACATTTCCAGCTCTTGAATGCCCCTGTGGTGGTGGGCAATGCCGAATCTTAACAGCTAAAACGGGCAAAAATATCGGCAGCCAATTCTATCGCTGTCCTGGAAATGAGGGAAGCTCTTGCGGTTTCTTCAAGTGGTGCAGTGAGAATACTGTGGCAGCTGGTAGTGTTCAAGGTGCACGAATCCCCAAGGTGGTTTATGGTTCAAGCGAGACAGGCAGCCAAGGGAGCTATGGTGTTAGGACTGGTTCTTCCTGTTACAAGTGCGGAAAGGAAGGGCATTGGGCAAGAGATTGCTCCGTTCCTTCAAACAATGTTCCGGTTGAATTTGGAGGACGGTCTGCTTCAACAAGCAGCTGCTATAAATGTGGAAAGACGGGGCACTGGGCCAGGGACTGTCCTTCTGGTTAA

Protein Analysis

371

Amino Acids

38.0

Weight (kDa)

8.5

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 116 - 131 6.2e-08 Zinc knuckle
Zn_ribbon_GRF PF06839 168 - 211 8.2e-16 GRF zinc finger
Zn_ribbon_GRF PF06839 243 - 284 1.9e-14 GRF zinc finger
zf-CCHC PF00098 320 - 335 5.9e-08 Zinc knuckle
zf-CCHC PF00098 354 - 370 9.3e-09 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016078)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G42860
fragaria_vesca FvH4_6g39844 FvH4_6g39844 FvH4_6g39844
malus_domestica MD17G1125500.v1.1
prunus_persica Prupe.3G190200_v2.0.a1
pyrus_communis pycom17g11770
rosa_chinensis RchiOBHm_Chr2g0154211
rosa_laevigata RLG00000020764
rosa_roxburghii Rroxscaffold_2G00094460
rosa_samantha Rh2AG507900 Rh2BG518900 Rh2CG493200 Rh2DG529800
rosa_wichuraiana Rw2G041710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 533
AccB1I GGYRCC 1 cut(s) 154
AccI GTMKAC 1 cut(s) 48
AccII CGCG 9 cut(s) 98, 149, 171, 227, 251, 268, 270, 384, 482
AclWI GGATC 3 cut(s) 22, 186, 523
AcsI RAATTY 3 cut(s) 178, 569, 1031
AfiI CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 737, 898
AgsI TTSAA 9 cut(s) 184, 355, 725, 838, 878, 912, 1014, 1031, 1053
AhdI GACNNNNNGTC 1 cut(s) 475
AjnI CCWGG 2 cut(s) 805, 1094
AluBI AGCT 8 cut(s) 504, 675, 719, 764, 822, 866, 936, 1062
AluI AGCT 8 cut(s) 504, 675, 719, 764, 822, 866, 936, 1062
Alw21I GWGCWC 2 cut(s) 537, 886
Alw26I GTCTC 2 cut(s) 221, 911
Alw44I GTGCAC 1 cut(s) 882
AlwI GGATC 3 cut(s) 22, 186, 523
AlwNI CAGNNNCTG 1 cut(s) 1101
AoxI GGCC 4 cut(s) 84, 303, 641, 1092
ApaLI GTGCAC 1 cut(s) 882
ApeKI GCWGC 8 cut(s) 173, 473, 504, 786, 863, 924, 1059, 1062
ApoI RAATTY 3 cut(s) 178, 569, 1031
AspLEI GCGC 5 cut(s) 108, 173, 232, 251, 270
AspS9I GGNCC 5 cut(s) 85, 303, 641, 684, 1092
AsuC2I CCSGG 2 cut(s) 18, 369
AsuHPI GGTGA 2 cut(s) 353, 529
AvaII GGWCC 1 cut(s) 684
BaeGI GKGCMC 2 cut(s) 886, 1089
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 537, 886
BbvI GCAGC 8 cut(s) 185, 460, 491, 798, 875, 936, 1049, 1071
BceAI ACGGC 8 cut(s) 62, 96, 128, 226, 290, 343, 451, 478
BcgI CGANNNNNNTGC 6 cut(s) 71, 105, 374, 408, 763, 797
BciT130I CCWGG 2 cut(s) 807, 1096
BcnI CCSGG 2 cut(s) 18, 369
BcoDI GTCTC 2 cut(s) 221, 911
BfoI RGCGCY 2 cut(s) 109, 233
BfuAI ACCTGC 1 cut(s) 533
BglI GCCNNNNNGGC 2 cut(s) 83, 311
Bme1390I CCNGG 4 cut(s) 18, 369, 807, 1096
Bme18I GGWCC 1 cut(s) 684
BmeRI GACNNNNNGTC 1 cut(s) 475
BmgT120I GGNCC 5 cut(s) 85, 303, 641, 684, 1092
BmiI GGNNCC 4 cut(s) 156, 304, 523, 686
BmrFI CCNGG 4 cut(s) 18, 369, 807, 1096
BmrI ACTGGG 2 cut(s) 385, 1099
BmsI GCATC 3 cut(s) 334, 382, 707
BmuI ACTGGG 2 cut(s) 385, 1099
BoxI GACNNNNGTC 1 cut(s) 213
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 806, 838
BpuMI CCSGG 2 cut(s) 18, 369
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 4 cut(s) 651, 894, 928, 1095
BsaWI WCCGGW 1 cut(s) 1024
BsaXI ACNNNNNCTCC 2 cut(s) 925, 955
Bsc4I CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 737, 898
Bse1I ACTGG 3 cut(s) 380, 955, 1094
Bse3DI GCAATG 1 cut(s) 752
BseBI CCWGG 2 cut(s) 807, 1096
BseDI CCNNGG 4 cut(s) 651, 894, 928, 1095
BseLI CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 737, 898
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 3 cut(s) 80, 486, 540
BseNI ACTGG 3 cut(s) 380, 955, 1094
BseRI GAGGAG 1 cut(s) 420
BseSI GKGCMC 2 cut(s) 886, 1089
BseXI GCAGC 8 cut(s) 185, 460, 491, 798, 875, 936, 1049, 1071
BsgI GTGCAG 1 cut(s) 865
Bsh1236I CGCG 9 cut(s) 98, 149, 171, 227, 251, 268, 270, 384, 482
BshFI GGCC 4 cut(s) 86, 305, 643, 1094
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 537, 886
BsiSI CCGG 3 cut(s) 18, 368, 1025
BslFI GGGAC 2 cut(s) 384, 1112
BslI CCNNNNNNNGG 6 cut(s) 67, 269, 272, 404, 737, 898
BsmAI GTCTC 2 cut(s) 221, 911
BsmFI GGGAC 2 cut(s) 384, 1112
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 4 cut(s) 86, 305, 643, 1094
Bso31I GGTCTC 1 cut(s) 221
Bsp1286I GDGCHC 4 cut(s) 382, 537, 886, 1089
Bsp143I GATC 3 cut(s) 27, 191, 515
Bsp19I CCATGG 1 cut(s) 651
Bsp68I TCGCGA 2 cut(s) 98, 384
BspANI GGCC 4 cut(s) 86, 305, 643, 1094
BspCNI CTCAG 3 cut(s) 79, 487, 541
BspFNI CGCG 9 cut(s) 98, 149, 171, 227, 251, 268, 270, 384, 482
BspLI GGNNCC 4 cut(s) 156, 304, 523, 686
BspMI ACCTGC 1 cut(s) 533
BspPI GGATC 3 cut(s) 22, 186, 523
BspT107I GGYRCC 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 752
BsrI ACTGG 3 cut(s) 380, 955, 1094
BssECI CCNNGG 4 cut(s) 651, 894, 928, 1095
BssMI GATC 3 cut(s) 27, 191, 515
BssT1I CCWWGG 3 cut(s) 651, 894, 928
Bst2UI CCWGG 2 cut(s) 807, 1096
Bst4CI ACNGT 3 cut(s) 859, 1044, 1103
BstC8I GCNNGC 2 cut(s) 349, 382
BstDEI CTNAG 4 cut(s) 66, 495, 549, 587
BstDSI CCRYGG 1 cut(s) 651
BstENI CCTNNNNNAGG 1 cut(s) 65
BstFNI CGCG 9 cut(s) 98, 149, 171, 227, 251, 268, 270, 384, 482
BstH2I RGCGCY 2 cut(s) 109, 233
BstHHI GCGC 5 cut(s) 108, 173, 232, 251, 270
BstKTI GATC 3 cut(s) 30, 194, 518
BstMAI GTCTC 2 cut(s) 221, 911
BstMBI GATC 3 cut(s) 27, 191, 515
BstMWI GCNNNNNNNGC 8 cut(s) 83, 311, 336, 357, 408, 479, 921, 933
BstNI CCWGG 2 cut(s) 807, 1096
BstPAI GACNNNNGTC 1 cut(s) 213
BstSCI CCNGG 4 cut(s) 16, 367, 805, 1094
BstSLI GKGCMC 2 cut(s) 886, 1089
BstUI CGCG 9 cut(s) 98, 149, 171, 227, 251, 268, 270, 384, 482
BstV1I GCAGC 8 cut(s) 185, 460, 491, 798, 875, 936, 1049, 1071
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 191, 515
BstYI RGATCY 2 cut(s) 191, 515
BsuRI GGCC 4 cut(s) 86, 305, 643, 1094
BtgI CCRYGG 1 cut(s) 651
BtgZI GCGATG 2 cut(s) 208, 405
BtsI GCAGTG 1 cut(s) 853
BtsIMutI CAGTG 3 cut(s) 373, 853, 1087
BtuMI TCGCGA 2 cut(s) 98, 384
BveI ACCTGC 1 cut(s) 533
Cac8I GCNNGC 2 cut(s) 349, 382
CaiI CAGNNNCTG 1 cut(s) 1101
CfoI GCGC 5 cut(s) 108, 173, 232, 251, 270
Cfr13I GGNCC 5 cut(s) 85, 303, 641, 684, 1092
CseI GACGC 3 cut(s) 138, 465, 479
CviAII CATG 2 cut(s) 89, 652
DdeI CTNAG 4 cut(s) 66, 495, 549, 587
DpnI GATC 3 cut(s) 29, 193, 517
DpnII GATC 3 cut(s) 27, 191, 515
DriI GACNNNNNGTC 1 cut(s) 475
Eam1105I GACNNNNNGTC 1 cut(s) 475
EciI GGCGGA 3 cut(s) 150, 429, 456
Eco130I CCWWGG 3 cut(s) 651, 894, 928
Eco24I GRGCYC 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 684
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 684
EcoRII CCWGG 2 cut(s) 805, 1094
EcoT14I CCWWGG 3 cut(s) 651, 894, 928
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 3 cut(s) 651, 894, 928
FaeI CATG 2 cut(s) 92, 655
FaiI YATR 8 cut(s) 90, 423, 450, 653, 696, 906, 939, 1068
FaqI GGGAC 2 cut(s) 384, 1112
FatI CATG 2 cut(s) 88, 651
FauI CCCGC 3 cut(s) 293, 310, 353
FblI GTMKAC 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 382
GlaI GCGC 5 cut(s) 107, 172, 231, 250, 269
HaeII RGCGCY 2 cut(s) 109, 233
HaeIII GGCC 4 cut(s) 86, 305, 643, 1094
HapII CCGG 3 cut(s) 18, 368, 1025
HgaI GACGC 3 cut(s) 138, 465, 479
HhaI GCGC 5 cut(s) 108, 173, 232, 251, 270
Hin1II CATG 2 cut(s) 92, 655
Hin6I GCGC 5 cut(s) 106, 171, 230, 249, 268
HinP1I GCGC 5 cut(s) 106, 171, 230, 249, 268
HinfI GANTC 6 cut(s) 197, 294, 556, 704, 753, 888
HpaII CCGG 3 cut(s) 18, 368, 1025
HphI GGTGA 2 cut(s) 353, 529
Hpy166II GTNNAC 2 cut(s) 49, 884
Hpy188I TCNGA 5 cut(s) 27, 196, 202, 259, 703
Hpy188III TCNNGA 5 cut(s) 97, 383, 494, 519, 722
Hpy8I GTNNAC 2 cut(s) 49, 884
Hpy99I CGWCG 9 cut(s) 44, 47, 56, 83, 149, 154, 212, 329, 473
HpyAV CCTTC 4 cut(s) 178, 974, 1020, 1116
HpyCH4III ACNGT 3 cut(s) 859, 1044, 1103
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 3 cut(s) 698, 846, 884
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 311, 336, 357, 408, 479, 921, 933
HpyF3I CTNAG 4 cut(s) 66, 495, 549, 587
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 2 cut(s) 92, 655
HspAI GCGC 5 cut(s) 106, 171, 230, 249, 268
Kzo9I GATC 3 cut(s) 27, 191, 515
LmnI GCTCC 2 cut(s) 933, 1007
Lsp1109I GCAGC 8 cut(s) 185, 460, 491, 798, 875, 936, 1049, 1071
LweI GCATC 3 cut(s) 334, 382, 707
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 962
MalI GATC 3 cut(s) 29, 193, 517
MboI GATC 3 cut(s) 27, 191, 515
MboII GAAGA 4 cut(s) 16, 565, 826, 948
MflI RGATCY 2 cut(s) 191, 515
MhlI GDGCHC 4 cut(s) 382, 537, 886, 1089
MluCI AATT 6 cut(s) 178, 430, 457, 569, 791, 1031
MlyI GAGTC 2 cut(s) 191, 303
MmeI TCCRAC 1 cut(s) 219
Mph1103I ATGCAT 1 cut(s) 700
MseI TTAA 2 cut(s) 758, 1114
MspA1I CMGCKG 2 cut(s) 866, 1062
MspI CCGG 3 cut(s) 18, 368, 1025
MspR9I CCNGG 4 cut(s) 18, 369, 807, 1096
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 2 cut(s) 807, 1096
MvnI CGCG 9 cut(s) 98, 149, 171, 227, 251, 268, 270, 384, 482
MwoI GCNNNNNNNGC 8 cut(s) 83, 311, 336, 357, 408, 479, 921, 933
NciI CCSGG 2 cut(s) 18, 369
NcoI CCATGG 1 cut(s) 651
NdeII GATC 3 cut(s) 27, 191, 515
NlaIII CATG 2 cut(s) 92, 655
NlaIV GGNNCC 4 cut(s) 156, 304, 523, 686
NruI TCGCGA 2 cut(s) 98, 384
NsiI ATGCAT 1 cut(s) 700
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 51, 303
PctI GAATGC 1 cut(s) 731
PfeI GAWTC 4 cut(s) 556, 704, 753, 888
PfoI TCCNGGA 1 cut(s) 805
PleI GAGTC 2 cut(s) 191, 302
PpsI GAGTC 2 cut(s) 191, 302
PpuMI RGGWCCY 1 cut(s) 684
PshAI GACNNNNGTC 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 805, 1094
PspGI CCWGG 2 cut(s) 805, 1094
PspN4I GGNNCC 4 cut(s) 156, 304, 523, 686
PspPI GGNCC 5 cut(s) 85, 303, 641, 684, 1092
PspPPI RGGWCCY 1 cut(s) 684
PstNI CAGNNNCTG 1 cut(s) 1101
PsuI RGATCY 2 cut(s) 191, 515
PvuII CAGCTG 2 cut(s) 866, 1062
RruI TCGCGA 2 cut(s) 98, 384
SaqAI TTAA 2 cut(s) 758, 1114
Sau3AI GATC 3 cut(s) 27, 191, 515
Sau96I GGNCC 5 cut(s) 85, 303, 641, 684, 1092
SchI GAGTC 2 cut(s) 191, 303
ScrFI CCNGG 4 cut(s) 18, 369, 807, 1096
SduI GDGCHC 4 cut(s) 382, 537, 886, 1089
SfaNI GCATC 3 cut(s) 334, 382, 707
SinI GGWCC 1 cut(s) 684
Sse9I AATT 6 cut(s) 178, 430, 457, 569, 791, 1031
StyD4I CCNGG 4 cut(s) 16, 367, 805, 1094
StyI CCWWGG 3 cut(s) 651, 894, 928
TaaI ACNGT 3 cut(s) 859, 1044, 1103
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 81, 559, 565
TasI AATT 6 cut(s) 178, 430, 457, 569, 791, 1031
TauI GCSGC 9 cut(s) 77, 111, 230, 273, 314, 317, 414, 441, 468
TfiI GAWTC 4 cut(s) 556, 704, 753, 888
Tru1I TTAA 2 cut(s) 758, 1114
Tru9I TTAA 2 cut(s) 758, 1114
TscAI CASTG 3 cut(s) 380, 853, 1094
TseI GCWGC 8 cut(s) 173, 473, 504, 786, 863, 924, 1059, 1062
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 23, 994
TspRI CASTG 3 cut(s) 380, 853, 1094
VneI GTGCAC 1 cut(s) 882
VpaK11BI GGWCC 1 cut(s) 684
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 3 cut(s) 178, 569, 1031
XcmI CCANNNNNNNNNTGG 1 cut(s) 935
XmiI GTMKAC 1 cut(s) 48
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.