Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G42860 |
| fragaria_vesca | FvH4_6g39844 FvH4_6g39844 FvH4_6g39844 |
| malus_domestica | MD17G1125500.v1.1 |
| prunus_persica | Prupe.3G190200_v2.0.a1 |
| pyrus_communis | pycom17g11770 |
| rosa_chinensis | RchiOBHm_Chr2g0154211 |
| rosa_laevigata | RLG00000020764 |
| rosa_roxburghii | Rroxscaffold_2G00094460 |
| rosa_samantha | Rh2AG507900 Rh2BG518900 Rh2CG493200 Rh2DG529800 |
| rosa_wichuraiana | Rw2G041710 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 539 |
| AccB1I | GGYRCC | 1 cut(s) | 154 |
| AccI | GTMKAC | 1 cut(s) | 48 |
| AccII | CGCG | 7 cut(s) | 98, 149, 227, 251, 268, 270, 384 |
| AclWI | GGATC | 2 cut(s) | 22, 529 |
| AcsI | RAATTY | 1 cut(s) | 575 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 67, 269, 272, 404, 743 |
| AgsI | TTSAA | 2 cut(s) | 355, 731 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 481 |
| AjnI | CCWGG | 2 cut(s) | 397, 811 |
| AluBI | AGCT | 4 cut(s) | 510, 681, 725, 770 |
| AluI | AGCT | 4 cut(s) | 510, 681, 725, 770 |
| Alw21I | GWGCWC | 1 cut(s) | 543 |
| Alw26I | GTCTC | 1 cut(s) | 221 |
| AlwI | GGATC | 2 cut(s) | 22, 529 |
| Ama87I | CYCGRG | 1 cut(s) | 316 |
| AoxI | GGCC | 4 cut(s) | 84, 303, 312, 647 |
| ApeKI | GCWGC | 3 cut(s) | 479, 510, 792 |
| ApoI | RAATTY | 1 cut(s) | 575 |
| AspLEI | GCGC | 4 cut(s) | 108, 232, 251, 270 |
| AspS9I | GGNCC | 4 cut(s) | 85, 303, 647, 690 |
| AsuC2I | CCSGG | 1 cut(s) | 18 |
| AsuHPI | GGTGA | 1 cut(s) | 353 |
| AvaI | CYCGRG | 1 cut(s) | 316 |
| AvaII | GGWCC | 1 cut(s) | 690 |
| BanI | GGYRCC | 1 cut(s) | 154 |
| BanII | GRGCYC | 1 cut(s) | 382 |
| BbsI | GAAGAC | 1 cut(s) | 11 |
| Bbv12I | GWGCWC | 1 cut(s) | 543 |
| BbvI | GCAGC | 3 cut(s) | 466, 497, 804 |
| BceAI | ACGGC | 7 cut(s) | 96, 128, 226, 290, 343, 457, 484 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 71, 105, 374, 408, 769, 803 |
| BciT130I | CCWGG | 2 cut(s) | 399, 813 |
| BcnI | CCSGG | 1 cut(s) | 18 |
| BcoDI | GTCTC | 1 cut(s) | 221 |
| BfoI | RGCGCY | 2 cut(s) | 109, 233 |
| BfuAI | ACCTGC | 1 cut(s) | 539 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 83, 311 |
| Bme1390I | CCNGG | 3 cut(s) | 18, 399, 813 |
| Bme18I | GGWCC | 1 cut(s) | 690 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 481 |
| BmeT110I | CYCGRG | 1 cut(s) | 316 |
| BmgT120I | GGNCC | 4 cut(s) | 85, 303, 647, 690 |
| BmiI | GGNNCC | 4 cut(s) | 156, 304, 529, 692 |
| BmrFI | CCNGG | 3 cut(s) | 18, 399, 813 |
| BmrI | ACTGGG | 1 cut(s) | 385 |
| BmsI | GCATC | 3 cut(s) | 334, 382, 713 |
| BmuI | ACTGGG | 1 cut(s) | 385 |
| BoxI | GACNNNNGTC | 1 cut(s) | 213 |
| BpiI | GAAGAC | 1 cut(s) | 11 |
| BpuMI | CCSGG | 1 cut(s) | 18 |
| BsaBI | GATNNNNATC | 1 cut(s) | 32 |
| BsaI | GGTCTC | 1 cut(s) | 221 |
| BsaJI | CCNNGG | 4 cut(s) | 81, 315, 397, 657 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 67, 269, 272, 404, 743 |
| Bse1I | ACTGG | 1 cut(s) | 380 |
| Bse3DI | GCAATG | 1 cut(s) | 758 |
| Bse8I | GATNNNNATC | 1 cut(s) | 32 |
| BseBI | CCWGG | 2 cut(s) | 399, 813 |
| BseDI | CCNNGG | 4 cut(s) | 81, 315, 397, 657 |
| BseJI | GATNNNNATC | 1 cut(s) | 32 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 67, 269, 272, 404, 743 |
| BseMI | GCAATG | 1 cut(s) | 758 |
| BseMII | CTCAG | 3 cut(s) | 80, 492, 546 |
| BseNI | ACTGG | 1 cut(s) | 380 |
| BseRI | GAGGAG | 2 cut(s) | 426, 429 |
| BseXI | GCAGC | 3 cut(s) | 466, 497, 804 |
| BseYI | CCCAGC | 1 cut(s) | 172 |
| Bsh1236I | CGCG | 7 cut(s) | 98, 149, 227, 251, 268, 270, 384 |
| BshFI | GGCC | 4 cut(s) | 86, 305, 314, 649 |
| BshNI | GGYRCC | 1 cut(s) | 154 |
| BsiHKAI | GWGCWC | 1 cut(s) | 543 |
| BsiHKCI | CYCGRG | 1 cut(s) | 316 |
| BsiSI | CCGG | 1 cut(s) | 18 |
| BslFI | GGGAC | 2 cut(s) | 384, 720 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 67, 269, 272, 404, 743 |
| BsmAI | GTCTC | 1 cut(s) | 221 |
| BsmFI | GGGAC | 2 cut(s) | 384, 720 |
| BsnI | GGCC | 4 cut(s) | 86, 305, 314, 649 |
| Bso31I | GGTCTC | 1 cut(s) | 221 |
| BsoBI | CYCGRG | 1 cut(s) | 316 |
| Bsp1286I | GDGCHC | 2 cut(s) | 382, 543 |
| Bsp143I | GATC | 3 cut(s) | 27, 191, 521 |
| Bsp19I | CCATGG | 1 cut(s) | 657 |
| Bsp68I | TCGCGA | 2 cut(s) | 98, 384 |
| BspANI | GGCC | 4 cut(s) | 86, 305, 314, 649 |
| BspCNI | CTCAG | 3 cut(s) | 79, 493, 547 |
| BspFNI | CGCG | 7 cut(s) | 98, 149, 227, 251, 268, 270, 384 |
| BspLI | GGNNCC | 4 cut(s) | 156, 304, 529, 692 |
| BspMI | ACCTGC | 1 cut(s) | 539 |
| BspPI | GGATC | 2 cut(s) | 22, 529 |
| BspT107I | GGYRCC | 1 cut(s) | 154 |
| BspTNI | GGTCTC | 1 cut(s) | 221 |
| BsrDI | GCAATG | 1 cut(s) | 758 |
| BsrI | ACTGG | 1 cut(s) | 380 |
| BssECI | CCNNGG | 4 cut(s) | 81, 315, 397, 657 |
| BssMI | GATC | 3 cut(s) | 27, 191, 521 |
| BssT1I | CCWWGG | 1 cut(s) | 657 |
| Bst2UI | CCWGG | 2 cut(s) | 399, 813 |
| Bst6I | CTCTTC | 1 cut(s) | 403 |
| BstC8I | GCNNGC | 2 cut(s) | 349, 382 |
| BstDEI | CTNAG | 4 cut(s) | 66, 501, 555, 593 |
| BstDSI | CCRYGG | 1 cut(s) | 657 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 65 |
| BstFNI | CGCG | 7 cut(s) | 98, 149, 227, 251, 268, 270, 384 |
| BstH2I | RGCGCY | 2 cut(s) | 109, 233 |
| BstHHI | GCGC | 4 cut(s) | 108, 232, 251, 270 |
| BstKTI | GATC | 3 cut(s) | 30, 194, 524 |
| BstMAI | GTCTC | 1 cut(s) | 221 |
| BstMBI | GATC | 3 cut(s) | 27, 191, 521 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 83, 311, 336, 357, 485 |
| BstNI | CCWGG | 2 cut(s) | 399, 813 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 213 |
| BstSCI | CCNGG | 3 cut(s) | 16, 397, 811 |
| BstUI | CGCG | 7 cut(s) | 98, 149, 227, 251, 268, 270, 384 |
| BstV1I | GCAGC | 3 cut(s) | 466, 497, 804 |
| BstV2I | GAAGAC | 1 cut(s) | 11 |
| BstX2I | RGATCY | 1 cut(s) | 521 |
| BstYI | RGATCY | 1 cut(s) | 521 |
| BsuRI | GGCC | 4 cut(s) | 86, 305, 314, 649 |
| BtgI | CCRYGG | 1 cut(s) | 657 |
| BtgZI | GCGATG | 2 cut(s) | 208, 405 |
| BtsI | GCAGTG | 1 cut(s) | 543 |
| BtsIMutI | CAGTG | 2 cut(s) | 373, 543 |
| BtuMI | TCGCGA | 2 cut(s) | 98, 384 |
| BveI | ACCTGC | 1 cut(s) | 539 |
| Cac8I | GCNNGC | 2 cut(s) | 349, 382 |
| CfoI | GCGC | 4 cut(s) | 108, 232, 251, 270 |
| Cfr13I | GGNCC | 4 cut(s) | 85, 303, 647, 690 |
| CseI | GACGC | 3 cut(s) | 138, 471, 485 |
| CviAII | CATG | 2 cut(s) | 89, 658 |
| DdeI | CTNAG | 4 cut(s) | 66, 501, 555, 593 |
| DpnI | GATC | 3 cut(s) | 29, 193, 523 |
| DpnII | GATC | 3 cut(s) | 27, 191, 521 |
| DriI | GACNNNNNGTC | 1 cut(s) | 481 |
| Eam1104I | CTCTTC | 1 cut(s) | 403 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 481 |
| EarI | CTCTTC | 1 cut(s) | 403 |
| EciI | GGCGGA | 4 cut(s) | 150, 417, 462, 475 |
| Eco130I | CCWWGG | 1 cut(s) | 657 |
| Eco24I | GRGCYC | 1 cut(s) | 382 |
| Eco31I | GGTCTC | 1 cut(s) | 221 |
| Eco47I | GGWCC | 1 cut(s) | 690 |
| Eco88I | CYCGRG | 1 cut(s) | 316 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 65 |
| EcoO109I | RGGNCCY | 1 cut(s) | 690 |
| EcoRII | CCWGG | 2 cut(s) | 397, 811 |
| EcoT14I | CCWWGG | 1 cut(s) | 657 |
| EcoT22I | ATGCAT | 1 cut(s) | 706 |
| EcoT38I | GRGCYC | 1 cut(s) | 382 |
| ErhI | CCWWGG | 1 cut(s) | 657 |
| FaeI | CATG | 2 cut(s) | 92, 661 |
| FaiI | YATR | 5 cut(s) | 90, 429, 456, 659, 702 |
| FaqI | GGGAC | 2 cut(s) | 384, 720 |
| FatI | CATG | 2 cut(s) | 88, 657 |
| FauI | CCCGC | 3 cut(s) | 293, 353, 413 |
| FblI | GTMKAC | 1 cut(s) | 48 |
| FriOI | GRGCYC | 1 cut(s) | 382 |
| GlaI | GCGC | 4 cut(s) | 107, 231, 250, 269 |
| GsaI | CCCAGC | 1 cut(s) | 176 |
| HaeII | RGCGCY | 2 cut(s) | 109, 233 |
| HaeIII | GGCC | 4 cut(s) | 86, 305, 314, 649 |
| HapII | CCGG | 1 cut(s) | 18 |
| HgaI | GACGC | 3 cut(s) | 138, 471, 485 |
| HhaI | GCGC | 4 cut(s) | 108, 232, 251, 270 |
| Hin1II | CATG | 2 cut(s) | 92, 661 |
| Hin6I | GCGC | 4 cut(s) | 106, 230, 249, 268 |
| HinP1I | GCGC | 4 cut(s) | 106, 230, 249, 268 |
| HinfI | GANTC | 6 cut(s) | 178, 197, 294, 562, 710, 759 |
| HpaII | CCGG | 1 cut(s) | 18 |
| HphI | GGTGA | 1 cut(s) | 353 |
| Hpy166II | GTNNAC | 1 cut(s) | 49 |
| Hpy188I | TCNGA | 6 cut(s) | 27, 183, 196, 202, 259, 709 |
| Hpy188III | TCNNGA | 7 cut(s) | 97, 318, 369, 383, 500, 525, 728 |
| Hpy8I | GTNNAC | 1 cut(s) | 49 |
| Hpy99I | CGWCG | 9 cut(s) | 41, 44, 47, 56, 149, 154, 212, 329, 479 |
| HpyAV | CCTTC | 1 cut(s) | 178 |
| HpyCH4IV | ACGT | 1 cut(s) | 45 |
| HpyCH4V | TGCA | 2 cut(s) | 548, 704 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 83, 311, 336, 357, 485 |
| HpyF3I | CTNAG | 4 cut(s) | 66, 501, 555, 593 |
| HpySE526I | ACGT | 1 cut(s) | 45 |
| Hsp92II | CATG | 2 cut(s) | 92, 661 |
| HspAI | GCGC | 4 cut(s) | 106, 230, 249, 268 |
| Kzo9I | GATC | 3 cut(s) | 27, 191, 521 |
| Lsp1109I | GCAGC | 3 cut(s) | 466, 497, 804 |
| LweI | GCATC | 3 cut(s) | 334, 382, 713 |
| MaeII | ACGT | 1 cut(s) | 45 |
| MalI | GATC | 3 cut(s) | 29, 193, 523 |
| MboI | GATC | 3 cut(s) | 27, 191, 521 |
| MboII | GAAGA | 3 cut(s) | 16, 420, 571 |
| MflI | RGATCY | 1 cut(s) | 521 |
| MhlI | GDGCHC | 2 cut(s) | 382, 543 |
| MluCI | AATT | 4 cut(s) | 436, 463, 575, 797 |
| MlyI | GAGTC | 2 cut(s) | 191, 303 |
| Mph1103I | ATGCAT | 1 cut(s) | 706 |
| MseI | TTAA | 1 cut(s) | 764 |
| MspI | CCGG | 1 cut(s) | 18 |
| MspR9I | CCNGG | 3 cut(s) | 18, 399, 813 |
| MvaI | CCWGG | 2 cut(s) | 399, 813 |
| MvnI | CGCG | 7 cut(s) | 98, 149, 227, 251, 268, 270, 384 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 83, 311, 336, 357, 485 |
| NciI | CCSGG | 1 cut(s) | 18 |
| NcoI | CCATGG | 1 cut(s) | 657 |
| NdeII | GATC | 3 cut(s) | 27, 191, 521 |
| NlaIII | CATG | 2 cut(s) | 92, 661 |
| NlaIV | GGNNCC | 4 cut(s) | 156, 304, 529, 692 |
| NmeAIII | GCCGAG | 1 cut(s) | 106 |
| NruI | TCGCGA | 2 cut(s) | 98, 384 |
| NsiI | ATGCAT | 1 cut(s) | 706 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 42, 48, 51, 303 |
| PfeI | GAWTC | 4 cut(s) | 178, 562, 710, 759 |
| PfoI | TCCNGGA | 1 cut(s) | 811 |
| PleI | GAGTC | 2 cut(s) | 191, 302 |
| PpsI | GAGTC | 2 cut(s) | 191, 302 |
| PpuMI | RGGWCCY | 1 cut(s) | 690 |
| PshAI | GACNNNNGTC | 1 cut(s) | 213 |
| Psp5II | RGGWCCY | 1 cut(s) | 690 |
| Psp6I | CCWGG | 2 cut(s) | 397, 811 |
| PspFI | CCCAGC | 1 cut(s) | 172 |
| PspGI | CCWGG | 2 cut(s) | 397, 811 |
| PspN4I | GGNNCC | 4 cut(s) | 156, 304, 529, 692 |
| PspPI | GGNCC | 4 cut(s) | 85, 303, 647, 690 |
| PspPPI | RGGWCCY | 1 cut(s) | 690 |
| PsuI | RGATCY | 1 cut(s) | 521 |
| RruI | TCGCGA | 2 cut(s) | 98, 384 |
| SaqAI | TTAA | 1 cut(s) | 764 |
| Sau3AI | GATC | 3 cut(s) | 27, 191, 521 |
| Sau96I | GGNCC | 4 cut(s) | 85, 303, 647, 690 |
| SchI | GAGTC | 2 cut(s) | 191, 303 |
| ScrFI | CCNGG | 3 cut(s) | 18, 399, 813 |
| SduI | GDGCHC | 2 cut(s) | 382, 543 |
| SetI | ASST | 9 cut(s) | 48, 72, 160, 512, 533, 571, 683, 727, 772 |
| SfaNI | GCATC | 3 cut(s) | 334, 382, 713 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 311 |
| SinI | GGWCC | 1 cut(s) | 690 |
| Sse9I | AATT | 4 cut(s) | 436, 463, 575, 797 |
| StyD4I | CCNGG | 3 cut(s) | 16, 397, 811 |
| StyI | CCWWGG | 1 cut(s) | 657 |
| TaiI | ACGT | 1 cut(s) | 48 |
| TaqI | TCGA | 3 cut(s) | 36, 565, 571 |
| TasI | AATT | 4 cut(s) | 436, 463, 575, 797 |
| TauI | GCSGC | 8 cut(s) | 77, 80, 111, 230, 273, 314, 447, 474 |
| TfiI | GAWTC | 4 cut(s) | 178, 562, 710, 759 |
| Tru1I | TTAA | 1 cut(s) | 764 |
| Tru9I | TTAA | 1 cut(s) | 764 |
| TscAI | CASTG | 2 cut(s) | 380, 550 |
| TseI | GCWGC | 3 cut(s) | 479, 510, 792 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 23 |
| TspRI | CASTG | 2 cut(s) | 380, 550 |
| VpaK11BI | GGWCC | 1 cut(s) | 690 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 65 |
| XapI | RAATTY | 1 cut(s) | 575 |
| XmiI | GTMKAC | 1 cut(s) | 48 |
| Zsp2I | ATGCAT | 1 cut(s) | 706 |