Prupe.3G237800_v2.0.a1

Cytokinin hydroxylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0014039
Physical Location & Seq
Reverse (-)
23290624 .. 23293642
3019 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G237800.1

Sequence Viewer

Length: 1557 bp
ATGGCCGCGATGCTCCTGACGACTCTGCTAGTCATATTGCTTAGCTTGTTAATAAGAGTTGCATATGACACTCTCTCATGCTACTTTTTAACCCCAAGACGCATCAAGAAAATAATGGAAAAGCAAGGGGTGCGTGGCCCTAAACCCCGCCCTCTCAACGGCAACATCTTGGACATGGCCTCCCTTGTCGCCAAATCAACTTCTCATGACATGCACACTATCAGCCATGACATCGTCGGCCGCCTCTTGCCCCATTACGTCCTCTGGTCGAAGCAATATGGAAAAAGGTTCATATATTGGACTGGAATTGAGCCCCGGATGTGCCTGGCCGAGACTGAGTTGATCAAGGAGCTTCTGTCCAAGTACAGCACCGTCTCTGGTAAATCCTGGCTTCAGCAGCAAGGCTCCAAGCATTTCATCGGCCGCGGCCTACTAATGGCCAACGGCGAAGACTGGTATCACCAACGCCACATCGTTGCACCGGCATTCATGGGAGATAGACTCAAGAGCTATGCGGGGTACATGGTGGAATGCACTAAGGAGATGCTCCAGTCCCTCCAAAATGAGATAGAGACTTCAGGAGGAAGGGAGTTTGAGATAGGAGAGTACATGACAAGGCTCACGGCAGACATAATTTCAAGAACAGAGTTCGATAGCAGCTACGAGAAGGGAAAACAGATTTTTCGACTGCTCACGGTTCTGCAGCATCTATGCTCCCAAGCAAGCAAGCATTTGTGCCTTCCCGGAAGCCGGTTTTTCCCAAGTAAATACAACAGAGAAATAAAATCCCTGAAAATGGAGGTGGAGAGGCTGCTAATGGAGATAATACAGAGTCGAAAGGACTGCGTGGAGATCGGCCGGAGCAGCTCTTATGGGAATGATTTGCTGGGGATGTTGCTTAATGAGATGCAGAAGAAGAGAGGGAATGGTTTTAGCATAAATTTGCAGCTGATCATGGATGAATGCAAGACGTTCTTCTTTGCAGGACATGAAACCACTGCCCTGCTTCTCACTTGGACTGTCATGTTACTAGCCAGCAACCCCTCTTGGCAAGAAAAGGTTAGGGCTGAGCTCAAGCAAGTCTGCAATGGTGGAACTCCCTCTGTTGATCATCTCCCCAAGCTCACTTTGTTAAATATGGTAATAAATGAATCACTGAGGCTGTATCCACCAGCAACTGTTCTGCCTCGAATGGCCTTTGAAGACATCAAACTTGGTGACCTCCATATTCCGAAGGGCCTATCTATATGGATACCCGTTCTGGCCATTCACCACAGTGAAGAATTATGGGGCAAAGATGCAAATGAATTTAACCCTGAAAGGTTTGCTTCCAAGTCATTCACCCCCGGGAGGTTTATCCCATTTGCTACTGGTCCTCGAAATTGTATTGGCCAATCTTTCGCCATGATGGAAGCTAAGATCATATTAGCCATGTTAATCTCCCGGTTTAGCTTCACGATTTCACCTAATTATCGTCATGCTCCCGTTATTGTCCTCACCATCAAACCTAAGTATGGGGTTCAAGTATGCATGACCCCTATAGACCCTTCGACTTAG

Protein Analysis

519

Amino Acids

58.91

Weight (kDa)

9.21

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67110 AT1G67110 AT5G38450
fragaria_vesca FvH4_6g45180
malus_domestica MD09G1087700.v1.1 MD17G1076700.v1.1
prunus_persica Prupe.3G237800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0162891
rosa_laevigata RLG00000021359
rosa_multiflora Rmu_sc0006129.1_g000009
rosa_roxburghii Rroxscaffold_2G00087740
rosa_rugosa Rorug02G0499100
rosa_samantha Rh2AG565000 Rh2BG577800 Rh2CG547500 Rh2DG587600
rosa_wichuraiana Rw2G046760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 8, 426
AciI CCGC 6 cut(s) 6, 148, 241, 424, 426, 515
AcoI YGGCCR 8 cut(s) 3, 238, 327, 421, 438, 856, 1263, 1390
AcsI RAATTY 2 cut(s) 940, 1307
AcuI CTGAAG 2 cut(s) 377, 561
AfaI GTAC 3 cut(s) 365, 521, 608
AfiI CCNNNNNNNGG 6 cut(s) 158, 436, 750, 796, 1350, 1514
AgsI TTSAA 3 cut(s) 639, 1202, 1523
AjnI CCWGG 2 cut(s) 324, 386
AleI CACNNNNGTG 1 cut(s) 1275
Alw21I GWGCWC 1 cut(s) 1074
Alw26I GTCTC 3 cut(s) 326, 379, 566
AlwNI CAGNNNCTG 1 cut(s) 1178
Ama87I CYCGRG 1 cut(s) 1346
ApeKI GCWGC 6 cut(s) 397, 657, 703, 811, 864, 946
ApoI RAATTY 2 cut(s) 940, 1307
AspS9I GGNCC 3 cut(s) 137, 1237, 1373
AsuC2I CCSGG 5 cut(s) 316, 744, 1347, 1348, 1444
AsuHPI GGTGA 6 cut(s) 452, 1229, 1262, 1333, 1455, 1489
AvaI CYCGRG 1 cut(s) 1346
AvaII GGWCC 1 cut(s) 1373
BalI TGGCCA 3 cut(s) 440, 1265, 1392
BanII GRGCYC 2 cut(s) 315, 1074
BbsI GAAGAC 2 cut(s) 456, 1209
Bbv12I GWGCWC 1 cut(s) 1074
BbvI GCAGC 6 cut(s) 409, 669, 715, 798, 876, 958
BccI CCATC 2 cut(s) 1402, 1508
BceAI ACGGC 3 cut(s) 175, 460, 639
BcgI CGANNNNNNTGC 2 cut(s) 825, 859
BciT130I CCWGG 2 cut(s) 326, 388
BciVI GTATCC 2 cut(s) 1176, 1245
BclI TGATCA 3 cut(s) 342, 951, 1108
BcnI CCSGG 5 cut(s) 316, 744, 1347, 1348, 1444
BcoDI GTCTC 3 cut(s) 326, 379, 566
BfaI CTAG 2 cut(s) 29, 1031
BfmI CTRYAG 2 cut(s) 701, 1539
BfuI GTATCC 2 cut(s) 1176, 1245
BlpI GCTNAGC 2 cut(s) 41, 1068
Bme1390I CCNGG 7 cut(s) 316, 326, 388, 744, 1347, 1348, 1444
Bme18I GGWCC 1 cut(s) 1373
BmeT110I CYCGRG 1 cut(s) 1346
BmgT120I GGNCC 3 cut(s) 137, 1237, 1373
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 7 cut(s) 316, 326, 388, 744, 1347, 1348, 1444
BmsI GCATC 5 cut(s) 111, 534, 715, 897, 1288
BpiI GAAGAC 2 cut(s) 456, 1209
BplI GAGNNNNNCTC 2 cut(s) 486, 518
BpmI CTGGAG 1 cut(s) 533
Bpu1102I GCTNAGC 2 cut(s) 41, 1068
BpuEI CTTGAG 2 cut(s) 488, 1058
BpuMI CCSGG 5 cut(s) 316, 744, 1347, 1348, 1444
BsaJI CCNNGG 4 cut(s) 314, 424, 1345, 1346
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 341, 371
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 436, 750, 796, 1350, 1514
Bse118I RCCGGY 2 cut(s) 481, 750
Bse1I ACTGG 4 cut(s) 307, 458, 550, 1375
Bse3DI GCAATG 1 cut(s) 1093
BseBI CCWGG 2 cut(s) 326, 388
BseDI CCNNGG 4 cut(s) 314, 424, 1345, 1346
BseGI GGATG 3 cut(s) 324, 897, 964
BseLI CCNNNNNNNGG 6 cut(s) 158, 436, 750, 796, 1350, 1514
BseMI GCAATG 1 cut(s) 1093
BseMII CTCAG 3 cut(s) 327, 1059, 1148
BseNI ACTGG 4 cut(s) 307, 458, 550, 1375
BseX3I CGGCCG 3 cut(s) 238, 421, 856
BseXI GCAGC 6 cut(s) 409, 669, 715, 798, 876, 958
BseYI CCCAGC 1 cut(s) 886
Bsh1236I CGCG 2 cut(s) 8, 426
Bsh1285I CGRYCG 3 cut(s) 241, 424, 859
BsiEI CGRYCG 3 cut(s) 241, 424, 859
BsiHKAI GWGCWC 1 cut(s) 1074
BsiHKCI CYCGRG 1 cut(s) 1346
BsiSI CCGG 7 cut(s) 316, 482, 744, 751, 859, 1347, 1444
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 6 cut(s) 158, 436, 750, 796, 1350, 1514
BsmAI GTCTC 3 cut(s) 326, 379, 566
BsmBI CGTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 538
BsmI GAATGC 3 cut(s) 485, 536, 968
BsoBI CYCGRG 1 cut(s) 1346
Bsp1286I GDGCHC 2 cut(s) 315, 1074
Bsp143I GATC 5 cut(s) 342, 852, 951, 1108, 1419
Bsp1720I GCTNAGC 2 cut(s) 41, 1068
BspACI CCGC 6 cut(s) 6, 148, 241, 424, 426, 515
BspCNI CTCAG 3 cut(s) 328, 1060, 1149
BspFNI CGCG 2 cut(s) 8, 426
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 406
BspMAI CTGCAG 1 cut(s) 705
BsrDI GCAATG 1 cut(s) 1093
BsrFI RCCGGY 2 cut(s) 481, 750
BsrI ACTGG 4 cut(s) 307, 458, 550, 1375
BssAI RCCGGY 2 cut(s) 481, 750
BssECI CCNNGG 4 cut(s) 314, 424, 1345, 1346
BssMI GATC 5 cut(s) 342, 852, 951, 1108, 1419
Bst2UI CCWGG 2 cut(s) 326, 388
Bst4CI ACNGT 5 cut(s) 373, 697, 1021, 1180, 1277
Bst6I CTCTTC 1 cut(s) 911
BstAPI GCANNNNNTGC 1 cut(s) 130
BstC8I GCNNGC 3 cut(s) 724, 728, 1036
BstDEI CTNAG 8 cut(s) 41, 336, 537, 1068, 1157, 1416, 1509, 1554
BstDSI CCRYGG 1 cut(s) 424
BstEII GGTNACC 1 cut(s) 1217
BstF5I GGATG 3 cut(s) 324, 897, 964
BstFNI CGCG 2 cut(s) 8, 426
BstKTI GATC 5 cut(s) 345, 855, 954, 1111, 1422
BstMAI GTCTC 3 cut(s) 326, 379, 566
BstMBI GATC 5 cut(s) 342, 852, 951, 1108, 1419
BstMCI CGRYCG 3 cut(s) 241, 424, 859
BstMWI GCNNNNNNNGC 3 cut(s) 130, 397, 864
BstNI CCWGG 2 cut(s) 326, 388
BstNSI RCATGY 1 cut(s) 214
BstPI GGTNACC 1 cut(s) 1217
BstSCI CCNGG 7 cut(s) 314, 324, 386, 742, 1345, 1346, 1442
BstSFI CTRYAG 2 cut(s) 701, 1539
BstUI CGCG 2 cut(s) 8, 426
BstV1I GCAGC 6 cut(s) 409, 669, 715, 798, 876, 958
BstV2I GAAGAC 2 cut(s) 456, 1209
BstZI CGGCCG 3 cut(s) 238, 421, 856
BsuI GTATCC 2 cut(s) 1176, 1245
BtgI CCRYGG 1 cut(s) 424
BtgZI GCGATG 1 cut(s) 23
BtsCI GGATG 3 cut(s) 324, 897, 964
BtsI GCAGTG 1 cut(s) 996
BtsIMutI CAGTG 3 cut(s) 996, 1154, 1282
Cac8I GCNNGC 3 cut(s) 724, 728, 1036
CaiI CAGNNNCTG 1 cut(s) 1178
CciI TCATGA 1 cut(s) 205
Cfr10I RCCGGY 2 cut(s) 481, 750
Cfr13I GGNCC 3 cut(s) 137, 1237, 1373
Cfr42I CCGCGG 1 cut(s) 427
Cfr9I CCCGGG 1 cut(s) 1346
CseI GACGC 1 cut(s) 108
Csp6I GTAC 3 cut(s) 364, 520, 607
CviQI GTAC 3 cut(s) 364, 520, 607
DdeI CTNAG 8 cut(s) 41, 336, 537, 1068, 1157, 1416, 1509, 1554
DpnI GATC 5 cut(s) 344, 854, 953, 1110, 1421
DpnII GATC 5 cut(s) 342, 852, 951, 1108, 1419
EaeI YGGCCR 8 cut(s) 3, 238, 327, 421, 438, 856, 1263, 1390
EagI CGGCCG 3 cut(s) 238, 421, 856
Eam1104I CTCTTC 1 cut(s) 911
EarI CTCTTC 1 cut(s) 911
Ecl136II GAGCTC 1 cut(s) 1072
EclXI CGGCCG 3 cut(s) 238, 421, 856
Eco24I GRGCYC 2 cut(s) 315, 1074
Eco47I GGWCC 1 cut(s) 1373
Eco52I CGGCCG 3 cut(s) 238, 421, 856
Eco53kI GAGCTC 1 cut(s) 1072
Eco57I CTGAAG 2 cut(s) 377, 561
Eco88I CYCGRG 1 cut(s) 1346
Eco91I GGTNACC 1 cut(s) 1217
EcoICRI GAGCTC 1 cut(s) 1072
EcoO109I RGGNCCY 1 cut(s) 1237
EcoO65I GGTNACC 1 cut(s) 1217
EcoRII CCWGG 2 cut(s) 324, 386
EcoT22I ATGCAT 1 cut(s) 1532
EcoT38I GRGCYC 2 cut(s) 315, 1074
Esp3I CGTCTC 1 cut(s) 379
FalI AAGNNNNNCTT 4 cut(s) 959, 991, 1312, 1344
FaqI GGGAC 1 cut(s) 538
FauI CCCGC 2 cut(s) 155, 508
FauNDI CATATG 1 cut(s) 64
FbaI TGATCA 3 cut(s) 342, 951, 1108
FokI GGATG 3 cut(s) 331, 904, 971
FriOI GRGCYC 2 cut(s) 315, 1074
FspBI CTAG 2 cut(s) 29, 1031
GsaI CCCAGC 1 cut(s) 890
GsuI CTGGAG 1 cut(s) 533
HapII CCGG 7 cut(s) 316, 482, 744, 751, 859, 1347, 1444
HgaI GACGC 1 cut(s) 108
HinfI GANTC 4 cut(s) 22, 501, 832, 1151
HpaII CCGG 7 cut(s) 316, 482, 744, 751, 859, 1347, 1444
HphI GGTGA 6 cut(s) 452, 1229, 1262, 1333, 1455, 1489
Hpy188I TCNGA 1 cut(s) 1233
Hpy188III TCNNGA 7 cut(s) 16, 106, 206, 505, 579, 639, 1456
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 5 cut(s) 579, 661, 749, 1228, 1557
HpyCH4III ACNGT 5 cut(s) 373, 697, 1021, 1180, 1277
HpyCH4IV ACGT 2 cut(s) 258, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 397, 864
HpyF3I CTNAG 8 cut(s) 41, 336, 537, 1068, 1157, 1416, 1509, 1554
HpySE526I ACGT 2 cut(s) 258, 971
Ksp22I TGATCA 3 cut(s) 342, 951, 1108
KspI CCGCGG 1 cut(s) 427
Kzo9I GATC 5 cut(s) 342, 852, 951, 1108, 1419
LmnI GCTCC 7 cut(s) 18, 349, 410, 552, 719, 861, 1486
Lsp1109I GCAGC 6 cut(s) 409, 669, 715, 798, 876, 958
LweI GCATC 5 cut(s) 111, 534, 715, 897, 1288
MaeI CTAG 2 cut(s) 29, 1031
MaeII ACGT 2 cut(s) 258, 971
MaeIII GTNAC 2 cut(s) 1026, 1217
MalI GATC 5 cut(s) 344, 854, 953, 1110, 1421
MboI GATC 5 cut(s) 342, 852, 951, 1108, 1419
MboII GAAGA 6 cut(s) 461, 925, 928, 967, 1214, 1292
MhlI GDGCHC 2 cut(s) 315, 1074
MlsI TGGCCA 3 cut(s) 440, 1265, 1392
MluCI AATT 7 cut(s) 306, 633, 940, 1283, 1307, 1381, 1468
MluNI TGGCCA 3 cut(s) 440, 1265, 1392
MlyI GAGTC 3 cut(s) 16, 495, 841
Mox20I TGGCCA 3 cut(s) 440, 1265, 1392
Mph1103I ATGCAT 1 cut(s) 1532
MscI TGGCCA 3 cut(s) 440, 1265, 1392
MseI TTAA 6 cut(s) 50, 89, 900, 1133, 1311, 1436
MslI CAYNNNNRTG 1 cut(s) 1275
Msp20I TGGCCA 3 cut(s) 440, 1265, 1392
MspA1I CMGCKG 2 cut(s) 426, 949
MspI CCGG 7 cut(s) 316, 482, 744, 751, 859, 1347, 1444
MspR9I CCNGG 7 cut(s) 316, 326, 388, 744, 1347, 1348, 1444
Mva1269I GAATGC 3 cut(s) 485, 536, 968
MvaI CCWGG 2 cut(s) 326, 388
MvnI CGCG 2 cut(s) 8, 426
MwoI GCNNNNNNNGC 3 cut(s) 130, 397, 864
NciI CCSGG 5 cut(s) 316, 744, 1347, 1348, 1444
NdeI CATATG 1 cut(s) 64
NdeII GATC 5 cut(s) 342, 852, 951, 1108, 1419
NlaIV GGNNCC 1 cut(s) 406
NmeAIII GCCGAG 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 1217
NsiI ATGCAT 1 cut(s) 1532
NspI RCATGY 1 cut(s) 214
OliI CACNNNNGTG 1 cut(s) 1275
PagI TCATGA 1 cut(s) 205
PctI GAATGC 3 cut(s) 485, 536, 968
PfeI GAWTC 1 cut(s) 1151
PflFI GACNNNGTC 1 cut(s) 233
PfoI TCCNGGA 1 cut(s) 742
PleI GAGTC 3 cut(s) 16, 495, 840
PpsI GAGTC 3 cut(s) 16, 495, 840
Psp124BI GAGCTC 1 cut(s) 1074
Psp6I CCWGG 2 cut(s) 324, 386
PspEI GGTNACC 1 cut(s) 1217
PspFI CCCAGC 1 cut(s) 886
PspGI CCWGG 2 cut(s) 324, 386
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 3 cut(s) 137, 1237, 1373
PstI CTGCAG 1 cut(s) 705
PstNI CAGNNNCTG 1 cut(s) 1178
PsyI GACNNNGTC 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 949
RsaI GTAC 3 cut(s) 365, 521, 608
RsaNI GTAC 3 cut(s) 364, 520, 607
RseI CAYNNNNRTG 1 cut(s) 1275
SacI GAGCTC 1 cut(s) 1074
SacII CCGCGG 1 cut(s) 427
SaqAI TTAA 6 cut(s) 50, 89, 900, 1133, 1311, 1436
Sau3AI GATC 5 cut(s) 342, 852, 951, 1108, 1419
Sau96I GGNCC 3 cut(s) 137, 1237, 1373
SchI GAGTC 3 cut(s) 16, 495, 841
ScrFI CCNGG 7 cut(s) 316, 326, 388, 744, 1347, 1348, 1444
SduI GDGCHC 2 cut(s) 315, 1074
SfaNI GCATC 5 cut(s) 111, 534, 715, 897, 1288
SfcI CTRYAG 2 cut(s) 701, 1539
Sfr303I CCGCGG 1 cut(s) 427
SgrBI CCGCGG 1 cut(s) 427
SinI GGWCC 1 cut(s) 1373
SmaI CCCGGG 1 cut(s) 1348
SmiMI CAYNNNNRTG 1 cut(s) 1275
SmlI CTYRAG 2 cut(s) 503, 1073
SmoI CTYRAG 2 cut(s) 503, 1073
Sse9I AATT 7 cut(s) 306, 633, 940, 1283, 1307, 1381, 1468
SsiI CCGC 6 cut(s) 6, 148, 241, 424, 426, 515
SspMI CTAG 2 cut(s) 29, 1031
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 7 cut(s) 314, 324, 386, 742, 1345, 1346, 1442
TaaI ACNGT 5 cut(s) 373, 697, 1021, 1180, 1277
TaiI ACGT 2 cut(s) 261, 974
TaqI TCGA 7 cut(s) 269, 651, 685, 835, 1189, 1378, 1550
TasI AATT 7 cut(s) 306, 633, 940, 1283, 1307, 1381, 1468
TatI WGTACW 2 cut(s) 363, 606
TauI GCSGC 4 cut(s) 8, 243, 426, 429
TfiI GAWTC 1 cut(s) 1151
Tru1I TTAA 6 cut(s) 50, 89, 900, 1133, 1311, 1436
Tru9I TTAA 6 cut(s) 50, 89, 900, 1133, 1311, 1436
TscAI CASTG 3 cut(s) 1003, 1161, 1282
TseFI GTSAC 1 cut(s) 1217
TseI GCWGC 6 cut(s) 397, 657, 703, 811, 864, 946
Tsp45I GTSAC 1 cut(s) 1217
TspDTI ATGAA 7 cut(s) 280, 406, 478, 975, 1005, 1164, 1320
TspMI CCCGGG 1 cut(s) 1346
TspRI CASTG 3 cut(s) 1003, 1161, 1282
Tth111I GACNNNGTC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 1373
XapI RAATTY 2 cut(s) 940, 1307
XceI RCATGY 1 cut(s) 214
XmaI CCCGGG 1 cut(s) 1346
XspI CTAG 2 cut(s) 29, 1031
Zsp2I ATGCAT 1 cut(s) 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.