Rorug02G0499100

Cytokinin hydroxylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0014039
Physical Location & Seq
Reverse (-)
63164406 .. 63167536
3131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0499100.1

Sequence Viewer

Length: 1572 bp
ATGGCTTCCTCATCAACAATGGCAGCGAACGGGGTTCTCACTCTCCGGTCATCCACGTATGCCAATGCTTCGAATCAAACCCAGAAGACGAATCCGGCCTCCGCGCCGAGAGGGCTCTCGTTCAGCGGCTCTCATCTCTCCGGGGCCAAAATCCCGACGACGTCTTTGAGGACTTCCCCCAACAACACTCAAAGGGCGCCATTGGTTGTGTCTCCCAAGGCCGTTTCGGATTCCAAGAACTCCCAGACGTGTCTTGATCCCGATGCGAGCAGAAGTGTGTTGGGGATTATACTGGGAGGTGGAGCCGGGACGAGGCTTTACCCGTTGACAAAGAAGCGTGCGAAGCCTGCTGTTCCATTGGGAGCGAATTACAGGCTGATTGATATTCCCGTCAGCAATTGCCTCAACAGCAACGTTTCCAAGATCTACGTCCTCACACAGTTCAATTCCGCTTCTCTTAATCGCCATCTGTCTCGGGCTTACGCCAGCAACATGGGAGGGTACAAAAATGAAGGCTTTGTTGAAGTGCTTGCGGCGCAGCAGAGTCCCGAGAATCCCAATTGGTTCCAGGGTACTGCAGATGCTGTGAGGCAGTATTTGTGGTTGTTTGAAGAACACAATGTGTTGGAGTTTTTGGTTCTTGCCGGGGACCATTTGTATCGGATGGACTATGAGAAGTTCATTCAGGCTCATAGAGAAACTGATGCAGATATCACTGTGGCTGCTCTCCCCATGGATGAGAAGCGTGCTACTGCCTTCGGTTTGATGAAGATTGATGATGAGGGTCGCATTATTGAGTTTGCTGAGAAGCCTAAAGGGGAGCAACTCAAAGCTATGAAGGTAGATACAACCATATTGGGTCTTGATGATGAGAGAGCTAAAGAGATGCCTTACATCGCCAGTATGGGTATATATGTTGTGAGCAAAAATGTGATGTTAGATCTGCTTCGAGAGAAGTTTCCTGGAGCAAATGATTTTGGGAGTGAAGTCATTCCTGGTGCAACCTCCATTGGGTTGAGGGTTCAAGCTTATCTGTATGATGGCTACTGGGAAGACATTGGTACCATTGAGGCTTTCTATAATGCTAATCTGGGGATAACTAAAAAGCCAATTCCAGATTTCAGCTTCTACGATCGTTCATCCCCGATCTACACCCAACCTCGGTATTTGCCCCCATCCAAAATGCTTGATGCTGATGTCACAGATAGTGTTATTGGCGAAGGTTGTGTGATAAAGAACTGTAAAATTCACCATTCTGTCGTTGGGCTTCGTTCTTGCATATCAGAAGGTGCTGTTATTGAAGACACATTACTGATGGGGGCAGACTACTATGAGACGGATGCTGACAGGAGGTTTCTGGCTGGAAGAGGCAGTGTTCCAATTGGTATCGGAAAGAATTCTCACATTAAGAGAGCTATAATTGACAAGAATGCTCGAATTGGGGACAATGTGAAGATTGTCAACAGTGACAGTGTGCAAGAAGCTGCAAGAGAAACAGATGGATATTTCATCAAAAGCGGGATTGTCACAGTGATCAAGGATGCCTTGATTCCTAGTGGAACTGTAATCTGA

Protein Analysis

523

Amino Acids

57.0

Weight (kDa)

6.73

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 95 - 368 1e-65 Nucleotidyl transferase
LbH_GLGC PF25247 388 - 522 8.3e-54 GLGC left-handed parallel beta-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67110 AT1G67110 AT5G38450
fragaria_vesca FvH4_6g45180
malus_domestica MD09G1087700.v1.1 MD17G1076700.v1.1
prunus_persica Prupe.3G237800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0162891
rosa_laevigata RLG00000021359
rosa_multiflora Rmu_sc0006129.1_g000009
rosa_roxburghii Rroxscaffold_2G00087740
rosa_rugosa Rorug02G0499100
rosa_samantha Rh2AG565000 Rh2BG577800 Rh2CG547500 Rh2DG587600
rosa_wichuraiana Rw2G046760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 164
Acc65I GGTACC 1 cut(s) 1061
AccB1I GGYRCC 2 cut(s) 196, 1061
AccII CGCG 1 cut(s) 104
AciI CCGC 5 cut(s) 102, 126, 450, 533, 1518
AclI AACGTT 1 cut(s) 414
AclWI GGATC 1 cut(s) 251
AcsI RAATTY 2 cut(s) 1245, 1396
AcyI GRCGYC 2 cut(s) 161, 197
AdeI CACNNNGTG 1 cut(s) 622
AfaI GTAC 3 cut(s) 503, 574, 1063
AfiI CCNNNNNNNGG 3 cut(s) 312, 1011, 1161
AflIII ACRYGT 1 cut(s) 248
AgsI TTSAA 5 cut(s) 445, 524, 611, 1025, 1301
AjiI CACGTC 1 cut(s) 249
AjnI CCWGG 3 cut(s) 567, 961, 994
AluBI AGCT 6 cut(s) 833, 878, 1028, 1125, 1415, 1484
AluI AGCT 6 cut(s) 833, 878, 1028, 1125, 1415, 1484
Alw26I GTCTC 3 cut(s) 216, 477, 1328
AlwI GGATC 1 cut(s) 251
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 2 cut(s) 474, 548
AoxI GGCC 3 cut(s) 96, 144, 219
ApeKI GCWGC 4 cut(s) 23, 538, 722, 1484
ApoI RAATTY 2 cut(s) 1245, 1396
ArsI GACNNNNNNTTYG 2 cut(s) 148, 180
Asp700I GAANNNNTTC 2 cut(s) 990, 1396
Asp718I GGTACC 1 cut(s) 1061
AspLEI GCGC 3 cut(s) 106, 199, 538
AspS9I GGNCC 2 cut(s) 144, 649
AsuC2I CCSGG 3 cut(s) 142, 307, 646
AsuHPI GGTGA 1 cut(s) 1241
AsuII TTCGAA 1 cut(s) 71
AvaI CYCGRG 2 cut(s) 474, 548
AvaII GGWCC 1 cut(s) 649
BaeI ACNNNNGTAYC 2 cut(s) 1045, 1078
BanI GGYRCC 2 cut(s) 196, 1061
BanII GRGCYC 1 cut(s) 117
BarI GAAGNNNNNNTAC 2 cut(s) 1293, 1325
BbsI GAAGAC 3 cut(s) 92, 1059, 1308
BbvI GCAGC 4 cut(s) 35, 550, 709, 1471
BccI CCATC 6 cut(s) 474, 658, 1034, 1183, 1309, 1493
BceAI ACGGC 1 cut(s) 206
BciT130I CCWGG 3 cut(s) 569, 963, 996
BclI TGATCA 1 cut(s) 1533
BcnI CCSGG 3 cut(s) 142, 307, 646
BcoDI GTCTC 3 cut(s) 216, 477, 1328
BfaI CTAG 1 cut(s) 1554
BfmI CTRYAG 1 cut(s) 576
BfoI RGCGCY 1 cut(s) 200
BglI GCCNNNNNGGC 1 cut(s) 112
BglII AGATCT 2 cut(s) 423, 940
BisI GCNGC 6 cut(s) 24, 127, 534, 539, 723, 1485
BlsI GCNGC 6 cut(s) 25, 128, 535, 540, 724, 1486
Bme1390I CCNGG 6 cut(s) 142, 307, 569, 646, 963, 996
Bme18I GGWCC 1 cut(s) 649
BmeT110I CYCGRG 2 cut(s) 474, 548
BmgBI CACGTC 1 cut(s) 249
BmgT120I GGNCC 2 cut(s) 144, 649
BmiI GGNNCC 6 cut(s) 145, 198, 304, 566, 650, 1063
BmrFI CCNGG 6 cut(s) 142, 307, 569, 646, 963, 996
BmrI ACTGGG 2 cut(s) 302, 1057
BmsI GCATC 7 cut(s) 253, 571, 694, 876, 1180, 1330, 1531
BmuI ACTGGG 2 cut(s) 302, 1057
BpiI GAAGAC 3 cut(s) 92, 1059, 1308
BpmI CTGGAG 1 cut(s) 984
Bpu14I TTCGAA 1 cut(s) 71
BpuMI CCSGG 3 cut(s) 142, 307, 646
BsaAI YACGTR 1 cut(s) 57
BsaHI GRCGYC 2 cut(s) 161, 197
BsaJI CCNNGG 6 cut(s) 141, 216, 568, 645, 732, 1160
BsaWI WCCGGW 1 cut(s) 45
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 1011, 1161
Bse1I ACTGG 3 cut(s) 297, 900, 1052
BseBI CCWGG 3 cut(s) 569, 963, 996
BseDI CCNNGG 6 cut(s) 141, 216, 568, 645, 732, 1160
BseGI GGATG 7 cut(s) 50, 669, 742, 1139, 1175, 1345, 1546
BseLI CCNNNNNNNGG 3 cut(s) 312, 1011, 1161
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 3 cut(s) 297, 900, 1052
BseXI GCAGC 4 cut(s) 35, 550, 709, 1471
Bsh1236I CGCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 98, 146, 221
BshNI GGYRCC 2 cut(s) 196, 1061
BsiEI CGRYCG 1 cut(s) 1135
BsiHKCI CYCGRG 2 cut(s) 474, 548
BsiSI CCGG 5 cut(s) 46, 95, 141, 306, 645
BslFI GGGAC 4 cut(s) 322, 531, 662, 1457
BslI CCNNNNNNNGG 3 cut(s) 312, 1011, 1161
BsmAI GTCTC 3 cut(s) 216, 477, 1328
BsmBI CGTCTC 1 cut(s) 1328
BsmFI GGGAC 4 cut(s) 322, 531, 662, 1457
BsmI GAATGC 1 cut(s) 1435
BsnI GGCC 3 cut(s) 98, 146, 221
BsoBI CYCGRG 2 cut(s) 474, 548
Bsp119I TTCGAA 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 5 cut(s) 102, 126, 450, 533, 1518
BspANI GGCC 3 cut(s) 98, 146, 221
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 6 cut(s) 145, 198, 304, 566, 650, 1063
BspMAI CTGCAG 1 cut(s) 580
BspPI GGATC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 71
BspT107I GGYRCC 2 cut(s) 196, 1061
BsrI ACTGG 3 cut(s) 297, 900, 1052
BssECI CCNNGG 6 cut(s) 141, 216, 568, 645, 732, 1160
BssMI GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
BssNI GRCGYC 2 cut(s) 161, 197
BssT1I CCWWGG 2 cut(s) 216, 732
Bst2UI CCWGG 3 cut(s) 569, 963, 996
Bst4CI ACNGT 7 cut(s) 441, 718, 1241, 1466, 1472, 1531, 1564
Bst6I CTCTTC 1 cut(s) 1360
BstACI GRCGYC 2 cut(s) 161, 197
BstBAI YACGTR 1 cut(s) 57
BstBI TTCGAA 1 cut(s) 71
BstC8I GCNNGC 6 cut(s) 268, 339, 348, 487, 531, 747
BstDEI CTNAG 1 cut(s) 804
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 7 cut(s) 50, 669, 742, 1139, 1175, 1345, 1546
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 200
BstHHI GCGC 3 cut(s) 106, 199, 538
BstKTI GATC 6 cut(s) 259, 426, 943, 1135, 1149, 1536
BstMAI GTCTC 3 cut(s) 216, 477, 1328
BstMBI GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 5 cut(s) 112, 343, 347, 408, 535
BstNI CCWGG 3 cut(s) 569, 963, 996
BstSCI CCNGG 6 cut(s) 140, 305, 567, 644, 961, 994
BstSFI CTRYAG 1 cut(s) 576
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 4 cut(s) 35, 550, 709, 1471
BstV2I GAAGAC 3 cut(s) 92, 1059, 1308
BstX2I RGATCY 2 cut(s) 423, 940
BstXI CCANNNNNNTGG 1 cut(s) 493
BstYI RGATCY 2 cut(s) 423, 940
BsuRI GGCC 3 cut(s) 98, 146, 221
BtgI CCRYGG 1 cut(s) 732
BtgZI GCGATG 1 cut(s) 880
BtrI CACGTC 1 cut(s) 249
BtsCI GGATG 7 cut(s) 50, 669, 742, 1139, 1175, 1345, 1546
BtsI GCAGTG 1 cut(s) 1378
BtsIMutI CAGTG 5 cut(s) 714, 1378, 1471, 1477, 1536
Cac8I GCNNGC 6 cut(s) 268, 339, 348, 487, 531, 747
CaiI CAGNNNCTG 1 cut(s) 584
CfoI GCGC 3 cut(s) 106, 199, 538
Cfr13I GGNCC 2 cut(s) 144, 649
Csp6I GTAC 3 cut(s) 502, 573, 1062
CviAII CATG 2 cut(s) 493, 733
CviQI GTAC 3 cut(s) 502, 573, 1062
DdeI CTNAG 1 cut(s) 804
DinI GGCGCC 1 cut(s) 198
DpnI GATC 6 cut(s) 258, 425, 942, 1134, 1148, 1535
DpnII GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
DraIII CACNNNGTG 1 cut(s) 622
Eam1104I CTCTTC 1 cut(s) 1360
EarI CTCTTC 1 cut(s) 1360
Eco130I CCWWGG 2 cut(s) 216, 732
Eco24I GRGCYC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 712
Eco47I GGWCC 1 cut(s) 649
Eco88I CYCGRG 2 cut(s) 474, 548
EcoRI GAATTC 1 cut(s) 1396
EcoRII CCWGG 3 cut(s) 567, 961, 994
EcoRV GATATC 1 cut(s) 712
EcoT14I CCWWGG 2 cut(s) 216, 732
EcoT38I GRGCYC 1 cut(s) 117
EgeI GGCGCC 1 cut(s) 198
EheI GGCGCC 1 cut(s) 198
ErhI CCWWGG 2 cut(s) 216, 732
Esp3I CGTCTC 1 cut(s) 1328
FaeI CATG 2 cut(s) 496, 736
FalI AAGNNNNNCTT 2 cut(s) 1529, 1561
FaqI GGGAC 4 cut(s) 322, 531, 662, 1457
FatI CATG 2 cut(s) 492, 732
FauI CCCGC 1 cut(s) 1511
FbaI TGATCA 1 cut(s) 1533
Fnu4HI GCNGC 6 cut(s) 24, 127, 534, 539, 723, 1485
FokI GGATG 7 cut(s) 37, 676, 749, 1126, 1162, 1352, 1553
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 6 cut(s) 24, 127, 534, 539, 723, 1485
FspBI CTAG 1 cut(s) 1554
GlaI GCGC 3 cut(s) 105, 198, 537
GluI GCNGC 6 cut(s) 24, 127, 534, 539, 723, 1485
GsuI CTGGAG 1 cut(s) 984
HaeII RGCGCY 1 cut(s) 200
HaeIII GGCC 3 cut(s) 98, 146, 221
HapII CCGG 5 cut(s) 46, 95, 141, 306, 645
HhaI GCGC 3 cut(s) 106, 199, 538
Hin1I GRCGYC 2 cut(s) 161, 197
Hin1II CATG 2 cut(s) 496, 736
Hin6I GCGC 3 cut(s) 104, 197, 536
HinP1I GCGC 3 cut(s) 104, 197, 536
HincII GTYRAC 2 cut(s) 327, 1462
HindII GTYRAC 2 cut(s) 327, 1462
HindIII AAGCTT 1 cut(s) 1026
HinfI GANTC 6 cut(s) 73, 91, 230, 544, 553, 1549
HpaII CCGG 5 cut(s) 46, 95, 141, 306, 645
HphI GGTGA 1 cut(s) 1241
Hpy166II GTNNAC 2 cut(s) 327, 1462
Hpy188I TCNGA 5 cut(s) 229, 663, 1285, 1391, 1571
Hpy188III TCNNGA 7 cut(s) 154, 254, 260, 548, 863, 950, 1115
Hpy8I GTNNAC 2 cut(s) 327, 1462
Hpy99I CGWCG 2 cut(s) 160, 163
HpyAV CCTTC 5 cut(s) 506, 766, 832, 1214, 1280
HpyCH4III ACNGT 7 cut(s) 441, 718, 1241, 1466, 1472, 1531, 1564
HpyCH4IV ACGT 5 cut(s) 56, 161, 248, 414, 429
HpyCH4V TGCA 6 cut(s) 578, 707, 1001, 1278, 1477, 1487
HpyF10VI GCNNNNNNNGC 5 cut(s) 112, 343, 347, 408, 535
HpyF3I CTNAG 1 cut(s) 804
HpySE526I ACGT 5 cut(s) 56, 161, 248, 414, 429
Hsp92I GRCGYC 2 cut(s) 161, 197
Hsp92II CATG 2 cut(s) 496, 736
HspAI GCGC 3 cut(s) 104, 197, 536
KasI GGCGCC 1 cut(s) 196
KpnI GGTACC 1 cut(s) 1065
Ksp22I TGATCA 1 cut(s) 1533
Kzo9I GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
LmnI GCTCC 4 cut(s) 302, 362, 820, 965
Lsp1109I GCAGC 4 cut(s) 35, 550, 709, 1471
LweI GCATC 7 cut(s) 253, 571, 694, 876, 1180, 1330, 1531
MaeI CTAG 1 cut(s) 1554
MaeII ACGT 5 cut(s) 56, 161, 248, 414, 429
MaeIII GTNAC 3 cut(s) 1198, 1466, 1525
MalI GATC 6 cut(s) 258, 425, 942, 1134, 1148, 1535
MboI GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
MboII GAAGA 7 cut(s) 97, 623, 781, 1064, 1313, 1377, 1465
MfeI CAATTG 3 cut(s) 397, 559, 1380
MflI RGATCY 2 cut(s) 423, 940
MhlI GDGCHC 1 cut(s) 117
Mly113I GGCGCC 1 cut(s) 197
MlyI GAGTC 1 cut(s) 553
MmeI TCCRAC 1 cut(s) 606
MroXI GAANNNNTTC 2 cut(s) 990, 1396
MseI TTAA 2 cut(s) 459, 1407
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 5 cut(s) 46, 95, 141, 306, 645
MspR9I CCNGG 6 cut(s) 142, 307, 569, 646, 963, 996
MunI CAATTG 3 cut(s) 397, 559, 1380
Mva1269I GAATGC 1 cut(s) 1435
MvaI CCWGG 3 cut(s) 569, 963, 996
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 5 cut(s) 112, 343, 347, 408, 535
NarI GGCGCC 1 cut(s) 197
NciI CCSGG 3 cut(s) 142, 307, 646
NcoI CCATGG 1 cut(s) 732
NdeII GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
NlaIII CATG 2 cut(s) 496, 736
NlaIV GGNNCC 6 cut(s) 145, 198, 304, 566, 650, 1063
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 3 cut(s) 1198, 1466, 1525
NspV TTCGAA 1 cut(s) 71
PctI GAATGC 1 cut(s) 1435
PdmI GAANNNNTTC 2 cut(s) 990, 1396
PfeI GAWTC 5 cut(s) 73, 91, 230, 553, 1549
PflFI GACNNNGTC 1 cut(s) 160
PfoI TCCNGGA 1 cut(s) 961
PkrI GCNGC 6 cut(s) 25, 128, 535, 540, 724, 1486
Ple19I CGATCG 1 cut(s) 1135
PleI GAGTC 1 cut(s) 552
PluTI GGCGCC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 552
Ppu21I YACGTR 1 cut(s) 57
Psp1406I AACGTT 1 cut(s) 414
Psp6I CCWGG 3 cut(s) 567, 961, 994
PspGI CCWGG 3 cut(s) 567, 961, 994
PspN4I GGNNCC 6 cut(s) 145, 198, 304, 566, 650, 1063
PspPI GGNCC 2 cut(s) 144, 649
PstI CTGCAG 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 2 cut(s) 423, 940
PsyI GACNNNGTC 1 cut(s) 160
PvuI CGATCG 1 cut(s) 1135
RsaI GTAC 3 cut(s) 503, 574, 1063
RsaNI GTAC 3 cut(s) 502, 573, 1062
SaqAI TTAA 2 cut(s) 459, 1407
SatI GCNGC 6 cut(s) 24, 127, 534, 539, 723, 1485
Sau3AI GATC 6 cut(s) 256, 423, 940, 1132, 1146, 1533
Sau96I GGNCC 2 cut(s) 144, 649
SchI GAGTC 1 cut(s) 553
ScrFI CCNGG 6 cut(s) 142, 307, 569, 646, 963, 996
SduI GDGCHC 1 cut(s) 117
SfaNI GCATC 7 cut(s) 253, 571, 694, 876, 1180, 1330, 1531
SfcI CTRYAG 1 cut(s) 576
SfoI GGCGCC 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 71
SinI GGWCC 1 cut(s) 649
SsiI CCGC 5 cut(s) 102, 126, 450, 533, 1518
SspDI GGCGCC 1 cut(s) 196
SspMI CTAG 1 cut(s) 1554
StyD4I CCNGG 6 cut(s) 140, 305, 567, 644, 961, 994
StyI CCWWGG 2 cut(s) 216, 732
TaaI ACNGT 7 cut(s) 441, 718, 1241, 1466, 1472, 1531, 1564
TaiI ACGT 5 cut(s) 59, 164, 251, 417, 432
TaqI TCGA 3 cut(s) 71, 949, 1435
TauI GCSGC 2 cut(s) 129, 536
TfiI GAWTC 5 cut(s) 73, 91, 230, 553, 1549
Tru1I TTAA 2 cut(s) 459, 1407
Tru9I TTAA 2 cut(s) 459, 1407
TscAI CASTG 5 cut(s) 721, 1378, 1471, 1477, 1536
TseFI GTSAC 3 cut(s) 1198, 1466, 1525
TseI GCWGC 4 cut(s) 23, 538, 722, 1484
Tsp45I GTSAC 3 cut(s) 1198, 1466, 1525
TspDTI ATGAA 6 cut(s) 525, 670, 782, 851, 1128, 1498
TspGWI ACGGA 1 cut(s) 1352
TspRI CASTG 5 cut(s) 721, 1378, 1471, 1477, 1536
Tth111I GACNNNGTC 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 649
XapI RAATTY 2 cut(s) 1245, 1396
XcmI CCANNNNNNNNNTGG 1 cut(s) 1259
XmnI GAANNNNTTC 2 cut(s) 990, 1396
XspI CTAG 1 cut(s) 1554
ZraI GACGTC 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.