RchiOBHm_Chr2g0162891

Cytokinin hydroxylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014039
Physical Location & Seq
Reverse (-)
78560468 .. 78564700
4233 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53111

Sequence Viewer

Length: 1530 bp
ATGGCCGCGGTGGCGCTAACAATTCTGTTGGTGATATTCCTTGGTTTACTGGTAAGAATTGCTTACGACACTCTCTCATGTTACTGGTTAACACCGAGACGCATCAAGAAGATCATGGAGAAGCAAGGAGTGCGTGGCCCGAAACCCCGGTTTCTGATCGGAAACATCCTCGACATGGCCTCCCTTGTCTCCAAGTCCACTTCTCAAGACATGCATACCATCGATCATGACATTGTTGGCCGCTTGTTGCCCCATTATCTTGCCTGGTCTAAAATATATGGGAAACGATTCATATATTGGAATGGAATTGAGCCCCGGATGTGTTTGACGGAGACTGAATTGATCAAGGAGCTTCTGTCCAAGTACAGCACCATATCTGGAAAATCATGGCTGCAACAACAAGGCTCTAAGCATTTTATTGGCCGGGGGTTACTAATGGCTAACGGTGACGACTGGTATCACCAGCGTCACATCGTTGCACCCGCATTCATGGGAGATAAACTCAAGAGCTATGCGGGATACATGGTGGAATGCACTAAGAAGATGCTCCATTCACTACAAACTGAGATGGATTCCGGGCAAGGTGAGTTCGAGATAAGCGAGTACATGACAAGACTCACCGCCGACATAATTTCCCGGACGGAGTTCGACAGCAGCTATGAGAAGGGAAAACAGATTTTCCGGCTACTCACCCTTTTGCAGAAACAATGCGCCCAAGCAAGCAAGCACCTTTGCGTTCCCGGAAGCCGGGAAATAAAATCTCTAAAGATGGAGGTGGAGAGGCTATTGATGGAAATTATACAGAGCCGGAAAGATTGCGTGGAGATTGGTCGGAACAACTCTTATGGGAATGATTTGCTAGGGATGTTGCTAAATGAGATGCAGAAAAAGAGAGGGAATGGATTTAGCTTTAATTTGCAGCTAATCATGGATGAATGCAAGACATTCTTCTTTGCAGGACATGAAACCACTGCCCTCTTGCTCACTTGGACAGTCATGTTATTAGCCAGCAACCCATCTTGGCAAGAAAAGGTTCGAGCTGAAGTGAAGCAAGTGTGCAATGGTGAAACTCCCTCTGTTGATCATCTATCCAAACTCGCCATGTTAAATATGGTAATAAATGAATCACTGAGGCTGTACCCACCAGCAACTGTTCTGCCTCGAATGGCTTTCGAAGACATCAAACTTGGTGACCTTCACATTCCAAAGGGGCTATCAGTATGGATTCCAGTCCTTGCCATTCACCACAGTAAAGAATTATGGGGTAAAGATGCAAATGAGTTTGACCCTGAGAGGTTTGCTTCCAAGTCATTCACACCAGGAAGGTTCATTCCTTTTGCTGCCGGTCCTCGAAATTGCATTGGTCAATCTTTTGCCATTATGGAAGCTAAGATCATATTAGCGATGTTAATCTCACGGTTTAGCTTCACGATTTCGCCAAGTTATCGCCATGCTCCCGTTATTGTGCTCACCATAAAGCCTAAGTATGGGGTTCAAGTATGCTTGAAACCTATTGATCCTGGAAATTAA

Protein Analysis

509

Amino Acids

57.82

Weight (kDa)

9.19

Isoelectric Point (pI)

40.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 90 - 481 1.7e-84 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67110 AT1G67110 AT5G38450
fragaria_vesca FvH4_6g45180
malus_domestica MD09G1087700.v1.1 MD17G1076700.v1.1
prunus_persica Prupe.3G237800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0162891
rosa_laevigata RLG00000021359
rosa_multiflora Rmu_sc0006129.1_g000009
rosa_roxburghii Rroxscaffold_2G00087740
rosa_rugosa Rorug02G0499100
rosa_samantha Rh2AG565000 Rh2BG577800 Rh2CG547500 Rh2DG587600
rosa_wichuraiana Rw2G046760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 8
AciI CCGC 6 cut(s) 6, 8, 241, 483, 515, 621
AclWI GGATC 1 cut(s) 1511
AcoI YGGCCR 3 cut(s) 3, 238, 421
AcuI CTGAAG 1 cut(s) 1062
AfaI GTAC 3 cut(s) 365, 605, 1139
AfiI CCNNNNNNNGG 3 cut(s) 175, 747, 1487
AgsI TTSAA 2 cut(s) 1496, 1507
AjnI CCWGG 3 cut(s) 263, 1318, 1519
AluBI AGCT 8 cut(s) 352, 510, 657, 909, 922, 1040, 1388, 1425
AluI AGCT 8 cut(s) 352, 510, 657, 909, 922, 1040, 1388, 1425
Alw21I GWGCWC 1 cut(s) 1470
Alw26I GTCTC 3 cut(s) 91, 193, 326
AlwI GGATC 1 cut(s) 1511
AlwNI CAGNNNCTG 1 cut(s) 1151
AoxI GGCC 5 cut(s) 3, 136, 177, 238, 421
ApeKI GCWGC 4 cut(s) 391, 654, 919, 1340
Asp700I GAANNNNTTC 2 cut(s) 287, 1032
AspLEI GCGC 2 cut(s) 16, 713
AspS9I GGNCC 2 cut(s) 137, 1346
AsuC2I CCSGG 7 cut(s) 148, 316, 425, 577, 637, 741, 749
AsuII TTCGAA 1 cut(s) 1173
AvaII GGWCC 1 cut(s) 1346
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 1182
Bbv12I GWGCWC 1 cut(s) 1470
BbvI GCAGC 4 cut(s) 378, 666, 931, 1327
BccI CCATC 5 cut(s) 227, 562, 763, 784, 1024
BciT130I CCWGG 3 cut(s) 265, 1320, 1521
BciVI GTATCC 1 cut(s) 512
BclI TGATCA 2 cut(s) 342, 1081
BcnI CCSGG 7 cut(s) 148, 316, 425, 577, 637, 741, 749
BcoDI GTCTC 3 cut(s) 91, 193, 326
BfaI CTAG 1 cut(s) 860
BfoI RGCGCY 1 cut(s) 17
BfuI GTATCC 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 6 cut(s) 6, 241, 392, 655, 920, 1341
BlsI GCNGC 6 cut(s) 7, 242, 393, 656, 921, 1342
Bme18I GGWCC 1 cut(s) 1346
BmgT120I GGNCC 2 cut(s) 137, 1346
BmsI GCATC 4 cut(s) 111, 534, 870, 1261
BpiI GAAGAC 1 cut(s) 1182
BplI GAGNNNNNCTC 2 cut(s) 486, 518
Bpu14I TTCGAA 1 cut(s) 1173
BpuEI CTTGAG 2 cut(s) 189, 488
BpuMI CCSGG 7 cut(s) 148, 316, 425, 577, 637, 741, 749
Bsa29I ATCGAT 1 cut(s) 222
BsaBI GATNNNNATC 1 cut(s) 1409
BsaJI CCNNGG 5 cut(s) 6, 40, 146, 314, 424
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 341, 371
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 747, 1487
Bse118I RCCGGY 1 cut(s) 1343
Bse1I ACTGG 4 cut(s) 54, 89, 458, 1229
Bse3DI GCAATG 1 cut(s) 1066
Bse8I GATNNNNATC 1 cut(s) 1409
BseBI CCWGG 3 cut(s) 265, 1320, 1521
BseCI ATCGAT 1 cut(s) 222
BseDI CCNNGG 5 cut(s) 6, 40, 146, 314, 424
BseGI GGATG 4 cut(s) 165, 324, 870, 937
BseJI GATNNNNATC 1 cut(s) 1409
BseLI CCNNNNNNNGG 3 cut(s) 175, 747, 1487
BseMI GCAATG 1 cut(s) 1066
BseMII CTCAG 3 cut(s) 555, 1121, 1281
BseNI ACTGG 4 cut(s) 54, 89, 458, 1229
BseXI GCAGC 4 cut(s) 378, 666, 931, 1327
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 5 cut(s) 5, 138, 179, 240, 423
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 1470
BslI CCNNNNNNNGG 3 cut(s) 175, 747, 1487
BsmAI GTCTC 3 cut(s) 91, 193, 326
BsmBI CGTCTC 1 cut(s) 91
BsmI GAATGC 3 cut(s) 485, 536, 941
BsnI GGCC 5 cut(s) 5, 138, 179, 240, 423
Bsp119I TTCGAA 1 cut(s) 1173
Bsp1286I GDGCHC 2 cut(s) 315, 1470
Bsp143I GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
BspACI CCGC 6 cut(s) 6, 8, 241, 483, 515, 621
BspANI GGCC 5 cut(s) 5, 138, 179, 240, 423
BspCNI CTCAG 3 cut(s) 556, 1122, 1282
BspDI ATCGAT 1 cut(s) 222
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 1 cut(s) 226
BspPI GGATC 1 cut(s) 1511
BspT104I TTCGAA 1 cut(s) 1173
BsrDI GCAATG 1 cut(s) 1066
BsrFI RCCGGY 1 cut(s) 1343
BsrI ACTGG 4 cut(s) 54, 89, 458, 1229
BssAI RCCGGY 1 cut(s) 1343
BssECI CCNNGG 5 cut(s) 6, 40, 146, 314, 424
BssMI GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 3 cut(s) 265, 1320, 1521
Bst4CI ACNGT 5 cut(s) 446, 994, 1153, 1250, 1419
BstAPI GCANNNNNTGC 1 cut(s) 130
BstBI TTCGAA 1 cut(s) 1173
BstC8I GCNNGC 3 cut(s) 721, 725, 1009
BstDEI CTNAG 7 cut(s) 408, 537, 564, 1130, 1290, 1389, 1482
BstDSI CCRYGG 1 cut(s) 6
BstEII GGTNACC 1 cut(s) 1190
BstF5I GGATG 4 cut(s) 165, 324, 870, 937
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 2 cut(s) 16, 713
BstKTI GATC 7 cut(s) 114, 159, 226, 345, 1084, 1395, 1519
BstMAI GTCTC 3 cut(s) 91, 193, 326
BstMBI GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
BstMWI GCNNNNNNNGC 2 cut(s) 11, 130
BstNI CCWGG 3 cut(s) 265, 1320, 1521
BstNSI RCATGY 1 cut(s) 214
BstPI GGTNACC 1 cut(s) 1190
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 4 cut(s) 378, 666, 931, 1327
BstV2I GAAGAC 1 cut(s) 1182
Bsu15I ATCGAT 1 cut(s) 222
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 5 cut(s) 5, 138, 179, 240, 423
BsuTUI ATCGAT 1 cut(s) 222
BtgI CCRYGG 1 cut(s) 6
BtgZI GCGATG 1 cut(s) 1418
BtsCI GGATG 4 cut(s) 165, 324, 870, 937
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 2 cut(s) 969, 1127
Cac8I GCNNGC 3 cut(s) 721, 725, 1009
CaiI CAGNNNCTG 1 cut(s) 1151
CciI TCATGA 1 cut(s) 226
CfoI GCGC 2 cut(s) 16, 713
Cfr10I RCCGGY 1 cut(s) 1343
Cfr13I GGNCC 2 cut(s) 137, 1346
Cfr42I CCGCGG 1 cut(s) 9
ClaI ATCGAT 1 cut(s) 222
CseI GACGC 2 cut(s) 108, 455
Csp6I GTAC 3 cut(s) 364, 604, 1138
CviQI GTAC 3 cut(s) 364, 604, 1138
DdeI CTNAG 7 cut(s) 408, 537, 564, 1130, 1290, 1389, 1482
DpnI GATC 7 cut(s) 113, 158, 225, 344, 1083, 1394, 1518
DpnII GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
EaeI YGGCCR 3 cut(s) 3, 238, 421
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 1346
Eco57I CTGAAG 1 cut(s) 1062
Eco91I GGTNACC 1 cut(s) 1190
EcoO65I GGTNACC 1 cut(s) 1190
EcoRII CCWGG 3 cut(s) 263, 1318, 1519
EcoT14I CCWWGG 1 cut(s) 40
EcoT22I ATGCAT 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 40
Esp3I CGTCTC 1 cut(s) 91
FalI AAGNNNNNCTT 4 cut(s) 46, 78, 932, 964
FauI CCCGC 2 cut(s) 490, 508
FbaI TGATCA 2 cut(s) 342, 1081
Fnu4HI GCNGC 6 cut(s) 6, 241, 392, 655, 920, 1341
FokI GGATG 4 cut(s) 152, 331, 877, 944
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 6 cut(s) 6, 241, 392, 655, 920, 1341
FspBI CTAG 1 cut(s) 860
GlaI GCGC 2 cut(s) 15, 712
GluI GCNGC 6 cut(s) 6, 241, 392, 655, 920, 1341
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 5 cut(s) 5, 138, 179, 240, 423
HgaI GACGC 2 cut(s) 108, 455
HhaI GCGC 2 cut(s) 16, 713
Hin6I GCGC 2 cut(s) 14, 711
HinP1I GCGC 2 cut(s) 14, 711
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HinfI GANTC 5 cut(s) 288, 572, 615, 1124, 1225
HpaI GTTAAC 1 cut(s) 90
Hpy166II GTNNAC 3 cut(s) 47, 90, 198
Hpy188I TCNGA 3 cut(s) 156, 161, 834
Hpy188III TCNNGA 7 cut(s) 106, 206, 227, 378, 505, 592, 1429
Hpy8I GTNNAC 3 cut(s) 47, 90, 198
HpyAV CCTTC 3 cut(s) 658, 1205, 1317
HpyCH4III ACNGT 5 cut(s) 446, 994, 1153, 1250, 1419
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 130
HpyF3I CTNAG 7 cut(s) 408, 537, 564, 1130, 1290, 1389, 1482
HspAI GCGC 2 cut(s) 14, 711
Ksp22I TGATCA 2 cut(s) 342, 1081
KspAI GTTAAC 1 cut(s) 90
KspI CCGCGG 1 cut(s) 9
Kzo9I GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
LmnI GCTCC 3 cut(s) 349, 552, 1459
Lsp1109I GCAGC 4 cut(s) 378, 666, 931, 1327
LweI GCATC 4 cut(s) 111, 534, 870, 1261
MaeI CTAG 1 cut(s) 860
MaeIII GTNAC 5 cut(s) 80, 429, 446, 467, 1190
MalI GATC 7 cut(s) 113, 158, 225, 344, 1083, 1394, 1518
MboI GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
MboII GAAGA 4 cut(s) 121, 553, 940, 1187
MhlI GDGCHC 2 cut(s) 315, 1470
MlyI GAGTC 1 cut(s) 609
MmeI TCCRAC 1 cut(s) 812
Mph1103I ATGCAT 1 cut(s) 216
MroXI GAANNNNTTC 2 cut(s) 287, 1032
MseI TTAA 5 cut(s) 89, 912, 1106, 1409, 1528
MspA1I CMGCKG 1 cut(s) 8
Mva1269I GAATGC 3 cut(s) 485, 536, 941
MvaI CCWGG 3 cut(s) 265, 1320, 1521
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 2 cut(s) 11, 130
NciI CCSGG 7 cut(s) 148, 316, 425, 577, 637, 741, 749
NdeII GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
NmuCI GTSAC 3 cut(s) 446, 467, 1190
NsiI ATGCAT 1 cut(s) 216
NspI RCATGY 1 cut(s) 214
NspV TTCGAA 1 cut(s) 1173
PagI TCATGA 1 cut(s) 226
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PctI GAATGC 3 cut(s) 485, 536, 941
PdmI GAANNNNTTC 2 cut(s) 287, 1032
PfeI GAWTC 4 cut(s) 288, 572, 1124, 1225
PfoI TCCNGGA 3 cut(s) 635, 739, 1519
PkrI GCNGC 6 cut(s) 7, 242, 393, 656, 921, 1342
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Psp6I CCWGG 3 cut(s) 263, 1318, 1519
PspEI GGTNACC 1 cut(s) 1190
PspGI CCWGG 3 cut(s) 263, 1318, 1519
PspPI GGNCC 2 cut(s) 137, 1346
PstNI CAGNNNCTG 1 cut(s) 1151
RsaI GTAC 3 cut(s) 365, 605, 1139
RsaNI GTAC 3 cut(s) 364, 604, 1138
SacII CCGCGG 1 cut(s) 9
SaqAI TTAA 5 cut(s) 89, 912, 1106, 1409, 1528
SatI GCNGC 6 cut(s) 6, 241, 392, 655, 920, 1341
Sau3AI GATC 7 cut(s) 111, 156, 223, 342, 1081, 1392, 1516
Sau96I GGNCC 2 cut(s) 137, 1346
SchI GAGTC 1 cut(s) 609
SduI GDGCHC 2 cut(s) 315, 1470
SfaNI GCATC 4 cut(s) 111, 534, 870, 1261
Sfr303I CCGCGG 1 cut(s) 9
SfuI TTCGAA 1 cut(s) 1173
SgrBI CCGCGG 1 cut(s) 9
SinI GGWCC 1 cut(s) 1346
SmlI CTYRAG 2 cut(s) 204, 503
SmoI CTYRAG 2 cut(s) 204, 503
SsiI CCGC 6 cut(s) 6, 8, 241, 483, 515, 621
SspMI CTAG 1 cut(s) 860
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 5 cut(s) 446, 994, 1153, 1250, 1419
TaqI TCGA 8 cut(s) 171, 222, 591, 648, 1036, 1162, 1173, 1351
TatI WGTACW 2 cut(s) 363, 603
TauI GCSGC 2 cut(s) 8, 243
TfiI GAWTC 4 cut(s) 288, 572, 1124, 1225
Tru1I TTAA 5 cut(s) 89, 912, 1106, 1409, 1528
Tru9I TTAA 5 cut(s) 89, 912, 1106, 1409, 1528
TscAI CASTG 2 cut(s) 976, 1134
TseFI GTSAC 3 cut(s) 446, 467, 1190
TseI GCWGC 4 cut(s) 391, 654, 919, 1340
Tsp45I GTSAC 3 cut(s) 446, 467, 1190
TspDTI ATGAA 6 cut(s) 280, 478, 948, 978, 1137, 1318
TspGWI ACGGA 2 cut(s) 344, 656
TspRI CASTG 2 cut(s) 976, 1134
VpaK11BI GGWCC 1 cut(s) 1346
XceI RCATGY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 1108
XmnI GAANNNNTTC 2 cut(s) 287, 1032
XspI CTAG 1 cut(s) 860
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.