Prupe.3G243700_v2.0.a1

EVI5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007170
Physical Location & Seq
Forward (+)
23624953 .. 23629415
4463 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G243700.1

Sequence Viewer

Length: 1071 bp
ATGGAAAAGAAACTGGAAGAAGATGACCATGAGGCAGGTCCAGTACCTGCACTGAGATTGGACAGATTTGGTTTTTTAAAGCAGGAACTTTCTCCTCAAGGTTTAACCAAGGGCAGGTCAGCCTATGAATATGAGAGAGAGGAAAGAAGGGTTAGAAAATGGAGGAAAATGATTGGTGTGGGAGGGAGTGATTGGAAACACTATATTAGGAGAAAACCTCATGTTGTTAAAAGGAGAATAAGGAAAGGAATCCCTGATTGTTTAAGGGGTCTGGTGTGGCAGTTGATCTCTGGCAGTCGAGACCTGCTGCTGATGAACCCTGGAGTTTATGAGCAATTAGTAATTTATGAGACATCAGCTTCAGAGCTGGATATAATTCGAGATATATCCCGTACCTTCCCTTCACATGTTTTCTTCCAGCAGCGACATGGTCCTGGTCAAAGGTCCCTCTACAATGTTTTGAAGGCATACTCTGTCTTTGACAGAGAAGTTGGATATGTTCAGGGAATGGGGTTTCTGGCTGGTCTATTGCTTCTTTATATGAGTGAAGAGGATGCATTTTGGTTATTAGTCGCATTACTCAAAGGAGCAGTCCATGCACCAATGGAAGGTTTATATCAGGTAGGGCTACCACTAGTACAACAATACCTTTTTCAGTTTGACCAGTTAGTAAAGGAGCACTTGCCAAAGATGGGAGAGCATTTTACTCAAGAGATGATAAATCCTAGCATGTATGCAAGCCAGTGGTTCATAACCGTTTTCTCCTATTCTTTCCCATTCCATTTGGCTCTTCGAATTTGGGATGTCTTTCTCTACGAGGGTGTTAGAATTGTTTTCAAAGTTGGTTTAGCCTTATTAAAGTATTGTCATGACGACTTGATAAAATTACCCTTTGAAAAACTTATTCATGCTTTGCGTAACTTCCCTGATGATGCAATGAATCCAGATACGTTACTTCCAATGGCTTACTCAATCAAGGTATCCAGGGGTTTGGAGGAAACAAGGCAGGAGTATGAGAAGAAGAATAGAAAGATCAATCAGTCTACAGAGAATGAGAAACAGTTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

357

Amino Acids

41.68

Weight (kDa)

8.92

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 4 cut(s) 26, 55, 105, 312
AccI GTMKAC 1 cut(s) 1043
AcsI RAATTY 1 cut(s) 795
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 3 cut(s) 45, 394, 639
AfiI CCNNNNNNNGG 3 cut(s) 114, 608, 692
AflIII ACRYGT 1 cut(s) 406
AgsI TTSAA 3 cut(s) 463, 838, 896
AhlI ACTAGT 1 cut(s) 634
AjnI CCWGG 3 cut(s) 319, 433, 983
AluBI AGCT 2 cut(s) 359, 367
AluI AGCT 2 cut(s) 359, 367
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 2 cut(s) 294, 344
AlwNI CAGNNNCTG 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 307, 421
ApoI RAATTY 1 cut(s) 795
ArsI GACNNNNNNTTYG 2 cut(s) 576, 608
AspS9I GGNCC 3 cut(s) 38, 431, 444
AsuII TTCGAA 1 cut(s) 793
AvaII GGWCC 3 cut(s) 38, 431, 444
BarI GAAGNNNNNNTAC 2 cut(s) 385, 417
Bbv12I GWGCWC 1 cut(s) 681
BbvI GCAGC 2 cut(s) 294, 433
BccI CCATC 1 cut(s) 685
BciT130I CCWGG 3 cut(s) 321, 435, 985
BciVI GTATCC 1 cut(s) 991
BcoDI GTCTC 2 cut(s) 294, 344
BcuI ACTAGT 1 cut(s) 634
BfaI CTAG 2 cut(s) 635, 726
BfmI CTRYAG 1 cut(s) 1044
BfuAI ACCTGC 4 cut(s) 26, 55, 105, 312
BfuI GTATCC 1 cut(s) 991
BisI GCNGC 2 cut(s) 308, 422
BlsI GCNGC 2 cut(s) 309, 423
Bme1390I CCNGG 3 cut(s) 321, 435, 985
Bme18I GGWCC 3 cut(s) 38, 431, 444
BmgT120I GGNCC 3 cut(s) 38, 431, 444
BmiI GGNNCC 1 cut(s) 446
BmrFI CCNGG 3 cut(s) 321, 435, 985
BmsI GCATC 2 cut(s) 544, 922
BplI GAGNNNNNCTC 2 cut(s) 202, 234
BpmI CTGGAG 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 793
BpuEI CTTGAG 2 cut(s) 81, 693
BsaI GGTCTC 1 cut(s) 294
BsaJI CCNNGG 3 cut(s) 108, 319, 984
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 608, 692
Bse1I ACTGG 4 cut(s) 18, 41, 664, 742
Bse3DI GCAATG 1 cut(s) 942
BseBI CCWGG 3 cut(s) 321, 435, 985
BseDI CCNNGG 3 cut(s) 108, 319, 984
BseGI GGATG 2 cut(s) 559, 808
BseLI CCNNNNNNNGG 3 cut(s) 114, 608, 692
BseMI GCAATG 1 cut(s) 942
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 4 cut(s) 18, 41, 664, 742
BseRI GAGGAG 1 cut(s) 84
BseXI GCAGC 2 cut(s) 294, 433
BsgI GTGCAG 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 681
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 3 cut(s) 114, 608, 692
BsmAI GTCTC 2 cut(s) 294, 344
BsmFI GGGAC 1 cut(s) 430
Bso31I GGTCTC 1 cut(s) 294
Bsp119I TTCGAA 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 681
Bsp143I GATC 2 cut(s) 285, 1032
BspCNI CTCAG 1 cut(s) 45
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 1 cut(s) 446
BspMI ACCTGC 4 cut(s) 26, 55, 105, 312
BspQI GCTCTTC 1 cut(s) 795
BspT104I TTCGAA 1 cut(s) 793
BspTNI GGTCTC 1 cut(s) 294
BsrDI GCAATG 1 cut(s) 942
BsrI ACTGG 4 cut(s) 18, 41, 664, 742
BssECI CCNNGG 3 cut(s) 108, 319, 984
BssMI GATC 2 cut(s) 285, 1032
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 3 cut(s) 321, 435, 985
Bst4CI ACNGT 2 cut(s) 757, 1062
Bst6I CTCTTC 2 cut(s) 543, 795
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 793
BstC8I GCNNGC 1 cut(s) 739
BstDEI CTNAG 1 cut(s) 53
BstF5I GGATG 2 cut(s) 559, 808
BstKTI GATC 2 cut(s) 288, 1035
BstMAI GTCTC 2 cut(s) 294, 344
BstMBI GATC 2 cut(s) 285, 1032
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstNI CCWGG 3 cut(s) 321, 435, 985
BstNSI RCATGY 2 cut(s) 410, 733
BstSCI CCNGG 3 cut(s) 319, 433, 983
BstSFI CTRYAG 1 cut(s) 1044
BstV1I GCAGC 2 cut(s) 294, 433
BstXI CCANNNNNNTGG 1 cut(s) 991
BsuI GTATCC 1 cut(s) 991
BtsCI GGATG 2 cut(s) 559, 808
BtsIMutI CAGTG 2 cut(s) 50, 749
BveI ACCTGC 4 cut(s) 26, 55, 105, 312
Cac8I GCNNGC 1 cut(s) 739
CaiI CAGNNNCTG 1 cut(s) 47
CciI TCATGA 1 cut(s) 868
Cfr13I GGNCC 3 cut(s) 38, 431, 444
Csp6I GTAC 3 cut(s) 44, 393, 638
CviAII CATG 8 cut(s) 29, 221, 407, 428, 596, 730, 869, 908
CviJI RGCY 9 cut(s) 122, 359, 367, 521, 628, 741, 788, 851, 965
CviKI_1 RGCY 9 cut(s) 122, 359, 367, 521, 628, 741, 788, 851, 965
CviQI GTAC 3 cut(s) 44, 393, 638
DdeI CTNAG 1 cut(s) 53
DpnI GATC 2 cut(s) 287, 1034
DpnII GATC 2 cut(s) 285, 1032
DraI TTTAAA 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 543, 795
EarI CTCTTC 2 cut(s) 543, 795
Eco130I CCWWGG 1 cut(s) 108
Eco31I GGTCTC 1 cut(s) 294
Eco47I GGWCC 3 cut(s) 38, 431, 444
Eco57I CTGAAG 1 cut(s) 345
EcoO109I RGGNCCY 1 cut(s) 444
EcoRII CCWGG 3 cut(s) 319, 433, 983
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 8 cut(s) 32, 224, 410, 431, 599, 733, 872, 911
FalI AAGNNNNNCTT 2 cut(s) 665, 697
FaqI GGGAC 1 cut(s) 430
FatI CATG 8 cut(s) 28, 220, 406, 427, 595, 729, 868, 907
FblI GTMKAC 1 cut(s) 1043
Fnu4HI GCNGC 2 cut(s) 308, 422
FokI GGATG 2 cut(s) 566, 815
Fsp4HI GCNGC 2 cut(s) 308, 422
FspBI CTAG 2 cut(s) 635, 726
GluI GCNGC 2 cut(s) 308, 422
GsuI CTGGAG 1 cut(s) 342
Hin1II CATG 8 cut(s) 32, 224, 410, 431, 599, 733, 872, 911
HinfI GANTC 2 cut(s) 249, 940
Hpy166II GTNNAC 1 cut(s) 1044
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 5 cut(s) 299, 380, 710, 869, 944
Hpy8I GTNNAC 1 cut(s) 1044
HpyAV CCTTC 5 cut(s) 141, 406, 411, 457, 602
HpyCH4III ACNGT 2 cut(s) 757, 1062
HpyCH4IV ACGT 1 cut(s) 950
HpyCH4V TGCA 6 cut(s) 50, 557, 599, 737, 935, 1066
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 1 cut(s) 950
Hsp92II CATG 8 cut(s) 32, 224, 410, 431, 599, 733, 872, 911
Kzo9I GATC 2 cut(s) 285, 1032
LguI GCTCTTC 1 cut(s) 795
LmnI GCTCC 2 cut(s) 587, 676
Lsp1109I GCAGC 2 cut(s) 294, 433
LweI GCATC 2 cut(s) 544, 922
MaeI CTAG 2 cut(s) 635, 726
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 2 cut(s) 917, 951
MalI GATC 2 cut(s) 287, 1034
MboI GATC 2 cut(s) 285, 1032
MboII GAAGA 7 cut(s) 29, 32, 406, 560, 782, 1030, 1033
MhlI GDGCHC 1 cut(s) 681
MluCI AATT 6 cut(s) 335, 342, 375, 795, 828, 884
MmeI TCCRAC 1 cut(s) 472
Mph1103I ATGCAT 1 cut(s) 559
MseI TTAA 5 cut(s) 77, 104, 228, 263, 857
MspR9I CCNGG 3 cut(s) 321, 435, 985
MvaI CCWGG 3 cut(s) 321, 435, 985
MwoI GCNNNNNNNGC 1 cut(s) 596
NdeII GATC 2 cut(s) 285, 1032
NlaIII CATG 8 cut(s) 32, 224, 410, 431, 599, 733, 872, 911
NlaIV GGNNCC 1 cut(s) 446
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 2 cut(s) 410, 733
NspV TTCGAA 1 cut(s) 793
PagI TCATGA 1 cut(s) 868
PciI ACATGT 1 cut(s) 406
PciSI GCTCTTC 1 cut(s) 795
PfeI GAWTC 2 cut(s) 249, 940
PflFI GACNNNGTC 1 cut(s) 429
PkrI GCNGC 2 cut(s) 309, 423
PpuMI RGGWCCY 1 cut(s) 444
PscI ACATGT 1 cut(s) 406
Psp5II RGGWCCY 1 cut(s) 444
Psp6I CCWGG 3 cut(s) 319, 433, 983
PspGI CCWGG 3 cut(s) 319, 433, 983
PspN4I GGNNCC 1 cut(s) 446
PspPI GGNCC 3 cut(s) 38, 431, 444
PspPPI RGGWCCY 1 cut(s) 444
PstNI CAGNNNCTG 1 cut(s) 47
PsyI GACNNNGTC 1 cut(s) 429
RsaI GTAC 3 cut(s) 45, 394, 639
RsaNI GTAC 3 cut(s) 44, 393, 638
SapI GCTCTTC 1 cut(s) 795
SaqAI TTAA 5 cut(s) 77, 104, 228, 263, 857
SatI GCNGC 2 cut(s) 308, 422
Sau3AI GATC 2 cut(s) 285, 1032
Sau96I GGNCC 3 cut(s) 38, 431, 444
ScrFI CCNGG 3 cut(s) 321, 435, 985
SduI GDGCHC 1 cut(s) 681
SfaNI GCATC 2 cut(s) 544, 922
SfcI CTRYAG 1 cut(s) 1044
SfuI TTCGAA 1 cut(s) 793
SinI GGWCC 3 cut(s) 38, 431, 444
SmlI CTYRAG 2 cut(s) 96, 708
SmoI CTYRAG 2 cut(s) 96, 708
SpeI ACTAGT 1 cut(s) 634
Sse9I AATT 6 cut(s) 335, 342, 375, 795, 828, 884
SspMI CTAG 2 cut(s) 635, 726
StyD4I CCNGG 3 cut(s) 319, 433, 983
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 2 cut(s) 757, 1062
TaiI ACGT 1 cut(s) 953
TaqI TCGA 3 cut(s) 298, 379, 793
TasI AATT 6 cut(s) 335, 342, 375, 795, 828, 884
TatI WGTACW 1 cut(s) 637
TfiI GAWTC 2 cut(s) 249, 940
Tru1I TTAA 5 cut(s) 77, 104, 228, 263, 857
Tru9I TTAA 5 cut(s) 77, 104, 228, 263, 857
TscAI CASTG 2 cut(s) 57, 749
TseI GCWGC 2 cut(s) 307, 421
TspDTI ATGAA 5 cut(s) 141, 329, 739, 896, 953
TspRI CASTG 2 cut(s) 57, 749
Tth111I GACNNNGTC 1 cut(s) 429
VpaK11BI GGWCC 3 cut(s) 38, 431, 444
XapI RAATTY 1 cut(s) 795
XceI RCATGY 2 cut(s) 410, 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 1043
XspI CTAG 2 cut(s) 635, 726
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.