pycom09g00670

EVI5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007170
Physical Location & Seq
Reverse (-)
497489 .. 499827
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g00670.7

Sequence Viewer

Length: 1074 bp
ATGGAAAACAAACTAGAAGAAGATGATGATCATGAGCCAGGTCCAGTTCCTCCACTGAGACTGGACAGATTTGGCTTCTTAAAGCCAGAAGTTTCTCCTCAAGGTTTAACCAAGAGCAGGTCAGCCTTTGAATATGAGAGAGAGGAAAGAAGAGTTAGAAAATGGAGGAAGATGATTGGGGTGGGAGGGAGTGATTGGAAACATTATATTAGGAGAAAACCTCACGTCGTTAAGAGGAGAATAAGGAAAGGAATTCCTGATTGTTTAAGGGGTCTTGTTTGGCAGTCGATCTCTGGAAGTCGGGACCTTTTGCTGATGAACCCTGGGGTTTACGAGCAATTAGTGATTTATGAGACCTCAGCTTCAGAGCTGGATATAATTCGAGATATATCGCGTACCTTCCCTTCACATATTTTCTTCCAGCAGAGACATGGCCCAGGTCAAAGGTCCCTCTACAATGTTTTGAAGGCGTACTCCGTCTTTGACAGAGAAGTTGGATATGTTCAGGGAATGGGATTTCTGGCTGGTCTCTTACTTCTTTATATGAGCGAAGAGGATGCATTTTGGTTATTAGTTGCATTACTCAAAGGAGCGGTCCATGCACCAATGGAAGGTTTATATCAGGTAGGGCTACCACTGGTACAGCAATACCTTTTTCAGCTTGATCAGTTGGTAAAGGAGCACTTGCCAAAGCTGGGGGAACATTTTACTCAAGAAATGATAAATCCTAGTATGTATGCAAGCCAGTGGTTCATAACCGTTTTCTCCTACTCTTTCCCCTTCCATTTGGCTCTTCGAATTTGGGATGTCTTTCTCTACGAGGGTGTGAGAATTGTCTTCAAAGTTGGTTTAGCCTTGTTAAAGTATTGCCATGACGACTTGACAAAATTACCATTCGAGAAACTTATTCATGCTCTGCGTAACTTCCCTGACGATGCAATGAATCCGGATACTTTACTTCCATTGGCTTACTCAATCAAGGTATCGCGGGGTTTGGAGGAAACAAGGCAGGAGTACGAGAAGAAGAACGGAAAGGAAGGTAAGTCTCCAGAGATTGATAAGCAGTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RabGAP-TBC

Protein Analysis

358

Amino Acids

41.55

Weight (kDa)

7.77

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 108
AccBSI CCGCTC 1 cut(s) 593
AccII CGCG 2 cut(s) 394, 988
AccIII TCCGGA 1 cut(s) 946
AciI CCGC 2 cut(s) 593, 988
AcsI RAATTY 2 cut(s) 252, 798
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 4 cut(s) 397, 473, 642, 1016
AfiI CCNNNNNNNGG 3 cut(s) 117, 611, 695
AgsI TTSAA 3 cut(s) 131, 466, 841
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 3 cut(s) 37, 322, 436
AluBI AGCT 4 cut(s) 362, 370, 661, 694
AluI AGCT 4 cut(s) 362, 370, 661, 694
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 5 cut(s) 52, 347, 421, 533, 1050
Aor13HI TCCGGA 1 cut(s) 946
AoxI GGCC 1 cut(s) 433
ApoI RAATTY 2 cut(s) 252, 798
ArsI GACNNNNNNTTYG 2 cut(s) 579, 611
AspS9I GGNCC 5 cut(s) 41, 304, 434, 447, 595
AsuII TTCGAA 1 cut(s) 796
AvaII GGWCC 4 cut(s) 41, 304, 447, 595
BarI GAAGNNNNNNTAC 2 cut(s) 388, 420
BbsI GAAGAC 1 cut(s) 829
Bbv12I GWGCWC 1 cut(s) 684
BbvCI CCTCAGC 1 cut(s) 358
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 3 cut(s) 39, 324, 438
BciVI GTATCC 1 cut(s) 943
BclI TGATCA 2 cut(s) 28, 664
BcoDI GTCTC 5 cut(s) 52, 347, 421, 533, 1050
BfaI CTAG 2 cut(s) 14, 729
BfuAI ACCTGC 1 cut(s) 108
BfuI GTATCC 1 cut(s) 943
Bme1390I CCNGG 3 cut(s) 39, 324, 438
Bme18I GGWCC 4 cut(s) 41, 304, 447, 595
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 5 cut(s) 41, 304, 434, 447, 595
BmiI GGNNCC 2 cut(s) 305, 449
BmrFI CCNGG 3 cut(s) 39, 324, 438
BmsI GCATC 2 cut(s) 547, 925
BpiI GAAGAC 1 cut(s) 829
BplI GAGNNNNNCTC 2 cut(s) 205, 237
BpmI CTGGAG 1 cut(s) 1032
Bpu10I CCTNAGC 1 cut(s) 358
Bpu14I TTCGAA 1 cut(s) 796
BpuEI CTTGAG 2 cut(s) 84, 696
BsaBI GATNNNNATC 1 cut(s) 27
BsaI GGTCTC 2 cut(s) 347, 533
BsaJI CCNNGG 3 cut(s) 322, 323, 436
BsaWI WCCGGW 1 cut(s) 946
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 611, 695
Bse1I ACTGG 4 cut(s) 44, 66, 642, 745
Bse3DI GCAATG 1 cut(s) 945
Bse8I GATNNNNATC 1 cut(s) 27
BseAI TCCGGA 1 cut(s) 946
BseBI CCWGG 3 cut(s) 39, 324, 438
BseDI CCNNGG 3 cut(s) 322, 323, 436
BseGI GGATG 2 cut(s) 562, 811
BseJI GATNNNNATC 1 cut(s) 27
BseLI CCNNNNNNNGG 3 cut(s) 117, 611, 695
BseMI GCAATG 1 cut(s) 945
BseMII CTCAG 2 cut(s) 47, 372
BseNI ACTGG 4 cut(s) 44, 66, 642, 745
BseRI GAGGAG 2 cut(s) 87, 250
BseYI CCCAGC 1 cut(s) 694
Bsh1236I CGCG 2 cut(s) 394, 988
BshFI GGCC 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 684
BsiSI CCGG 1 cut(s) 947
BslFI GGGAC 2 cut(s) 317, 433
BslI CCNNNNNNNGG 3 cut(s) 117, 611, 695
BsmAI GTCTC 5 cut(s) 52, 347, 421, 533, 1050
BsmFI GGGAC 2 cut(s) 317, 433
BsnI GGCC 1 cut(s) 435
Bso31I GGTCTC 2 cut(s) 347, 533
Bsp119I TTCGAA 1 cut(s) 796
Bsp1286I GDGCHC 1 cut(s) 684
Bsp13I TCCGGA 1 cut(s) 946
Bsp143I GATC 3 cut(s) 28, 288, 664
BspACI CCGC 2 cut(s) 593, 988
BspANI GGCC 1 cut(s) 435
BspCNI CTCAG 2 cut(s) 48, 371
BspEI TCCGGA 1 cut(s) 946
BspFNI CGCG 2 cut(s) 394, 988
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 305, 449
BspMI ACCTGC 1 cut(s) 108
BspQI GCTCTTC 1 cut(s) 798
BspT104I TTCGAA 1 cut(s) 796
BspTNI GGTCTC 2 cut(s) 347, 533
BsrBI CCGCTC 1 cut(s) 593
BsrDI GCAATG 1 cut(s) 945
BsrI ACTGG 4 cut(s) 44, 66, 642, 745
BssECI CCNNGG 3 cut(s) 322, 323, 436
BssMI GATC 3 cut(s) 28, 288, 664
Bst2UI CCWGG 3 cut(s) 39, 324, 438
Bst4CI ACNGT 1 cut(s) 760
Bst6I CTCTTC 3 cut(s) 145, 546, 798
BstBI TTCGAA 1 cut(s) 796
BstC8I GCNNGC 1 cut(s) 742
BstDEI CTNAG 2 cut(s) 56, 358
BstF5I GGATG 2 cut(s) 562, 811
BstFNI CGCG 2 cut(s) 394, 988
BstKTI GATC 3 cut(s) 31, 291, 667
BstMAI GTCTC 5 cut(s) 52, 347, 421, 533, 1050
BstMBI GATC 3 cut(s) 28, 288, 664
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 3 cut(s) 39, 324, 438
BstSCI CCNGG 3 cut(s) 37, 322, 436
BstUI CGCG 2 cut(s) 394, 988
BstV2I GAAGAC 1 cut(s) 829
BsuI GTATCC 1 cut(s) 943
BsuRI GGCC 1 cut(s) 435
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 2 cut(s) 562, 811
BtsIMutI CAGTG 3 cut(s) 53, 635, 752
BveI ACCTGC 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 742
CciI TCATGA 1 cut(s) 31
Cfr13I GGNCC 5 cut(s) 41, 304, 434, 447, 595
Csp6I GTAC 4 cut(s) 396, 472, 641, 1015
CviAII CATG 5 cut(s) 32, 431, 599, 872, 911
CviQI GTAC 4 cut(s) 396, 472, 641, 1015
DdeI CTNAG 2 cut(s) 56, 358
DpnI GATC 3 cut(s) 30, 290, 666
DpnII GATC 3 cut(s) 28, 288, 664
Eam1104I CTCTTC 3 cut(s) 145, 546, 798
EarI CTCTTC 3 cut(s) 145, 546, 798
Eco31I GGTCTC 2 cut(s) 347, 533
Eco47I GGWCC 4 cut(s) 41, 304, 447, 595
Eco57I CTGAAG 1 cut(s) 348
EcoO109I RGGNCCY 2 cut(s) 304, 447
EcoRI GAATTC 1 cut(s) 252
EcoRII CCWGG 3 cut(s) 37, 322, 436
EcoT22I ATGCAT 1 cut(s) 562
FaeI CATG 5 cut(s) 35, 434, 602, 875, 914
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 2 cut(s) 317, 433
FatI CATG 5 cut(s) 31, 430, 598, 871, 910
FauI CCCGC 1 cut(s) 981
FbaI TGATCA 2 cut(s) 28, 664
FokI GGATG 2 cut(s) 569, 818
FspBI CTAG 2 cut(s) 14, 729
GsaI CCCAGC 1 cut(s) 698
GsuI CTGGAG 1 cut(s) 1032
HaeIII GGCC 1 cut(s) 435
HapII CCGG 1 cut(s) 947
Hin1II CATG 5 cut(s) 35, 434, 602, 875, 914
HinfI GANTC 1 cut(s) 943
HpaII CCGG 1 cut(s) 947
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 9 cut(s) 32, 257, 294, 302, 383, 713, 898, 947, 1049
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 6 cut(s) 409, 414, 460, 605, 790, 1031
HpyCH4III ACNGT 1 cut(s) 760
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 5 cut(s) 560, 578, 602, 740, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 2 cut(s) 56, 358
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 5 cut(s) 35, 434, 602, 875, 914
Kpn2I TCCGGA 1 cut(s) 946
Ksp22I TGATCA 2 cut(s) 28, 664
Kzo9I GATC 3 cut(s) 28, 288, 664
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 2 cut(s) 590, 679
LweI GCATC 2 cut(s) 547, 925
MaeI CTAG 2 cut(s) 14, 729
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 920
MalI GATC 3 cut(s) 30, 290, 666
MbiI CCGCTC 1 cut(s) 593
MboI GATC 3 cut(s) 28, 288, 664
MhlI GDGCHC 1 cut(s) 684
MluCI AATT 6 cut(s) 252, 338, 378, 798, 831, 887
MmeI TCCRAC 2 cut(s) 475, 1047
Mph1103I ATGCAT 1 cut(s) 562
MroI TCCGGA 1 cut(s) 946
MseI TTAA 5 cut(s) 80, 107, 231, 266, 860
MspI CCGG 1 cut(s) 947
MspR9I CCNGG 3 cut(s) 39, 324, 438
MvaI CCWGG 3 cut(s) 39, 324, 438
MvnI CGCG 2 cut(s) 394, 988
MwoI GCNNNNNNNGC 1 cut(s) 599
NdeII GATC 3 cut(s) 28, 288, 664
NlaIII CATG 5 cut(s) 35, 434, 602, 875, 914
NlaIV GGNNCC 2 cut(s) 305, 449
NsiI ATGCAT 1 cut(s) 562
NspV TTCGAA 1 cut(s) 796
PagI TCATGA 1 cut(s) 31
PasI CCCWGGG 1 cut(s) 323
PciSI GCTCTTC 1 cut(s) 798
PfeI GAWTC 1 cut(s) 943
PpuMI RGGWCCY 2 cut(s) 304, 447
Psp5II RGGWCCY 2 cut(s) 304, 447
Psp6I CCWGG 3 cut(s) 37, 322, 436
PspFI CCCAGC 1 cut(s) 694
PspGI CCWGG 3 cut(s) 37, 322, 436
PspN4I GGNNCC 2 cut(s) 305, 449
PspPI GGNCC 5 cut(s) 41, 304, 434, 447, 595
PspPPI RGGWCCY 2 cut(s) 304, 447
RsaI GTAC 4 cut(s) 397, 473, 642, 1016
RsaNI GTAC 4 cut(s) 396, 472, 641, 1015
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 5 cut(s) 80, 107, 231, 266, 860
Sau3AI GATC 3 cut(s) 28, 288, 664
Sau96I GGNCC 5 cut(s) 41, 304, 434, 447, 595
ScrFI CCNGG 3 cut(s) 39, 324, 438
SduI GDGCHC 1 cut(s) 684
SfaNI GCATC 2 cut(s) 547, 925
SfuI TTCGAA 1 cut(s) 796
SinI GGWCC 4 cut(s) 41, 304, 447, 595
SmlI CTYRAG 2 cut(s) 99, 711
SmoI CTYRAG 2 cut(s) 99, 711
Sse9I AATT 6 cut(s) 252, 338, 378, 798, 831, 887
SsiI CCGC 2 cut(s) 593, 988
SspMI CTAG 2 cut(s) 14, 729
StyD4I CCNGG 3 cut(s) 37, 322, 436
TaaI ACNGT 1 cut(s) 760
TaiI ACGT 1 cut(s) 228
TaqI TCGA 4 cut(s) 287, 382, 796, 897
TasI AATT 6 cut(s) 252, 338, 378, 798, 831, 887
TfiI GAWTC 1 cut(s) 943
Tru1I TTAA 5 cut(s) 80, 107, 231, 266, 860
Tru9I TTAA 5 cut(s) 80, 107, 231, 266, 860
TscAI CASTG 3 cut(s) 60, 642, 752
TspDTI ATGAA 4 cut(s) 332, 742, 899, 956
TspGWI ACGGA 2 cut(s) 466, 1044
TspRI CASTG 3 cut(s) 60, 642, 752
VpaK11BI GGWCC 4 cut(s) 41, 304, 447, 595
XapI RAATTY 2 cut(s) 252, 798
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XspI CTAG 2 cut(s) 14, 729
Zsp2I ATGCAT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.