Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp04

Orthogroup ID

OG0014215

Physical Location & Seq

Genomic Coordinates Reverse (-)

6384546 .. 6385865

Gene Structure 1320 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.4G117800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1320 bp

ATGGAAGCTTTTCATCCTACTATCACCTTTCCCTTCTCCTACACCTTCAATACTACTAGTAGTGGACATGGTGGCATTGCTGAAAATATCAACACATCCAGTCCTAGTCCATGGATGAATAGTAGGATATGGAGTAAGCTCCCACACCGGCTGCTCGACCGTGTCATCGCCTTTCTTCCACCACCGGCCTTTTTTCGAGCTCGATGTGTGTGCAAGAGATGGTATGCTCTGTTGTTCTCCAACACCTTTCTTGAACTGTACCTCCAAGTATCTCCTCGCCGCCATTGGTTCCTCTTCTTCAAGCACAAACGCCTCAAGAGCAGCTACATATACAGGAACAACAATATTGGAGGAAGCCATGGTGACAACAACAGGGCTGGTACTGATTGTGAAGGGTATCTTTTTGATCCTTATGAACTTGCATGGTACCGCCTTTGTTTTGCTCTGCTTCCCTCCGGGTTCTCCCCTGCTTCTTCGTCAGGTGGTTTAATCTGTTGGGTTTCTGATGAGGCTGGTCCGAAGACTCTCATTCTGTGCAACCCAATTGTTGGTTCTCTGACTCAATTGCCTCCAACTCTAAGATCAAGGCTCTTCCCTTCCATAGGCTTAAGTGTTAGCCCTTCATCCATTGATGTCACTGTTGCTGGAGATGACTTGATTTCTCCATATGCTGTGAAAAACTTGACTGCAGAGAGCTTTCATATTGATGGAGGTGGGTTCTTTTCATTATGGGGCACCAATTCTTCTCTACCGAAGGTCTGCAATTTTGAATCAGGTCAAATGGTTCATGTTCAGGGAAAATTCTACTGCATGAACTATAGCCCTTTCAGTGTGTTGGCTTATGATGTGGCCGCAAATAACTGGTGGGAGATTAAGGCTCCCATGAAAAGGCATCTGAGGTCTCCAAGCTTGGTGGAGAGCAAGGGAAAGCTGCTGCTGGTTTCTGCAGTTAACAAGAGTAAGCTCAATGTGCCAAAAAGCCTGAGGCTTTGGGGCTTGCAGGCTTGTGGAAAAACATGGATTGAGATGGAAAGAATGCCACAGCAGCTTTATGTTCAATTTGCAGAGCTGGAAAATGGAAATGGGTTTCATTGTGTTGTCCATGGAGAGTTCCTTACCATAATGATCCCAGGTTCTGACAAGGCTTTGCTGTTTGATATGTGCCGGAAAAGGTGGCAATGGATCCCTCCATGCCCTTATGTTCATGCTGGGATAGTTGGTGATTTGCATGGTTTTGCTTATGAGCCCCAACTTGCCACACCAGTCACAGGGCTTCTTGATCAGTTGACTACAATTCCATTTCAGCCTTTCACTGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009987 GO:0010468 GO:0010498 GO:0010556 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031146 GO:0031323 GO:0031326 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051603 GO:0060255 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box

Protein Analysis

440

Amino Acids

49.27

Weight (kDa)

8.92

Isoelectric Point (pI)

52.86

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30950
fragaria_vesca	FvH4_3g03990 FvH4_3g12960
malus_domestica	MD05G1246800.v1.1 MD10G1227400.v1.1
prunus_persica	Prupe.4G117600_v2.0.a1 Prupe.4G117800_v2.0.a1
pyrus_communis	pycom05g22270
rosa_chinensis	RchiOBHm_Chr5g0021071
rosa_laevigata	RLG00000032581
rosa_multiflora	Rmu_ssc0000244.1_g000018
rosa_roxburghii	Rroxscaffold_1G00057390
rosa_rugosa	Rorug05G0064100
rosa_samantha	Rh5BG153400 Rh5CG166300 Rh5DG153000
rosa_wichuraiana	Rw5G013660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	426
AccB1I	GGYRCC	2 cut(s)	426, 734
AccB7I	CCANNNNNTGG	1 cut(s)	548
AciI	CCGC	3 cut(s)	280, 430, 852
AclWI	GGATC	4 cut(s)	401, 1120, 1177, 1190
AcoI	YGGCCR	1 cut(s)	849
AcsI	RAATTY	1 cut(s)	800
AfaI	GTAC	3 cut(s)	260, 382, 428
AfiI	CCNNNNNNNGG	5 cut(s)	548, 602, 888, 1268, 1313
AflII	CTTAAG	1 cut(s)	607
AgsI	TTSAA	5 cut(s)	49, 254, 301, 770, 1058
AhlI	ACTAGT	1 cut(s)	56
AjnI	CCWGG	1 cut(s)	1129
AjuI	GAANNNNNNNTTGG	2 cut(s)	535, 567
AloI	GAACNNNNNNTCC	2 cut(s)	246, 278
Alw21I	GWGCWC	1 cut(s)	202
Alw26I	GTCTC	1 cut(s)	906
AlwI	GGATC	4 cut(s)	401, 1120, 1177, 1190
AlwNI	CAGNNNCTG	1 cut(s)	1136
AoxI	GGCC	2 cut(s)	186, 849
ApeKI	GCWGC	5 cut(s)	151, 321, 931, 934, 1045
ApoI	RAATTY	1 cut(s)	800
Asp700I	GAANNNNTTC	1 cut(s)	9
Asp718I	GGTACC	1 cut(s)	426
AspS9I	GGNCC	1 cut(s)	515
AsuC2I	CCSGG	1 cut(s)	457
AsuHPI	GGTGA	3 cut(s)	16, 374, 1232
AvaII	GGWCC	1 cut(s)	515
AxyI	CCTNAGG	1 cut(s)	983
BaeGI	GKGCMC	1 cut(s)	737
BamHI	GGATCC	1 cut(s)	1182
BanI	GGYRCC	2 cut(s)	426, 734
BanII	GRGCYC	2 cut(s)	202, 1248
BbsI	GAAGAC	1 cut(s)	527
Bbv12I	GWGCWC	1 cut(s)	202
BbvI	GCAGC	5 cut(s)	138, 333, 918, 921, 1057
BccI	CCATC	3 cut(s)	213, 701, 1021
BcgI	CGANNNNNNTGC	2 cut(s)	192, 226
BciT130I	CCWGG	1 cut(s)	1131
BclI	TGATCA	1 cut(s)	1279
BcnI	CCSGG	1 cut(s)	457
BcoDI	GTCTC	1 cut(s)	906
BcuI	ACTAGT	1 cut(s)	56
BfaI	CTAG	2 cut(s)	57, 105
BfmI	CTRYAG	3 cut(s)	687, 817, 945
BfrI	CTTAAG	1 cut(s)	607
BisI	GCNGC	7 cut(s)	152, 280, 322, 852, 932, 935, 1046
BlsI	GCNGC	7 cut(s)	153, 281, 323, 853, 933, 936, 1047
Bme1390I	CCNGG	2 cut(s)	457, 1131
Bme18I	GGWCC	1 cut(s)	515
BmgT120I	GGNCC	1 cut(s)	515
BmiI	GGNNCC	5 cut(s)	290, 428, 736, 879, 1184
BmrFI	CCNGG	2 cut(s)	457, 1131
BmsI	GCATC	1 cut(s)	901
BpiI	GAAGAC	1 cut(s)	527
BpmI	CTGGAG	1 cut(s)	666
BpuEI	CTTGAG	1 cut(s)	299
BpuMI	CCSGG	1 cut(s)	457
BsaI	GGTCTC	1 cut(s)	906
BsaJI	CCNNGG	4 cut(s)	110, 358, 1102, 1129
BsaXI	ACNNNNNCTCC	2 cut(s)	246, 276
Bsc4I	CCNNNNNNNGG	5 cut(s)	548, 602, 888, 1268, 1313
Bse118I	RCCGGY	2 cut(s)	147, 184
Bse1I	ACTGG	4 cut(s)	99, 866, 1262, 1318
Bse21I	CCTNAGG	1 cut(s)	983
Bse3DI	GCAATG	2 cut(s)	75, 1184
BseBI	CCWGG	1 cut(s)	1131
BseDI	CCNNGG	4 cut(s)	110, 358, 1102, 1129
BseGI	GGATG	4 cut(s)	13, 95, 120, 623
BseLI	CCNNNNNNNGG	5 cut(s)	548, 602, 888, 1268, 1313
BseMI	GCAATG	2 cut(s)	75, 1184
BseMII	CTCAG	2 cut(s)	887, 974
BseNI	ACTGG	4 cut(s)	99, 866, 1262, 1318
BseRI	GAGGAG	1 cut(s)	264
BseSI	GKGCMC	1 cut(s)	737
BseXI	GCAGC	5 cut(s)	138, 333, 918, 921, 1057
BseYI	CCCAGC	1 cut(s)	1208
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	2 cut(s)	188, 851
BshNI	GGYRCC	2 cut(s)	426, 734
BsiEI	CGRYCG	1 cut(s)	160
BsiHKAI	GWGCWC	1 cut(s)	202
BsiSI	CCGG	4 cut(s)	148, 185, 456, 1165
BslI	CCNNNNNNNGG	5 cut(s)	548, 602, 888, 1268, 1313
BsmAI	GTCTC	1 cut(s)	906
BsmI	GAATGC	1 cut(s)	1041
BsnI	GGCC	2 cut(s)	188, 851
Bso31I	GGTCTC	1 cut(s)	906
Bsp1286I	GDGCHC	3 cut(s)	202, 737, 1248
Bsp143I	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
Bsp19I	CCATGG	3 cut(s)	110, 358, 1102
BspACI	CCGC	3 cut(s)	280, 430, 852
BspANI	GGCC	2 cut(s)	188, 851
BspCNI	CTCAG	2 cut(s)	888, 975
BspLI	GGNNCC	5 cut(s)	290, 428, 736, 879, 1184
BspMAI	CTGCAG	2 cut(s)	691, 949
BspPI	GGATC	4 cut(s)	401, 1120, 1177, 1190
BspQI	GCTCTTC	1 cut(s)	596
BspT107I	GGYRCC	2 cut(s)	426, 734
BspTI	CTTAAG	1 cut(s)	607
BspTNI	GGTCTC	1 cut(s)	906
BsrDI	GCAATG	2 cut(s)	75, 1184
BsrFI	RCCGGY	2 cut(s)	147, 184
BsrI	ACTGG	4 cut(s)	99, 866, 1262, 1318
BssAI	RCCGGY	2 cut(s)	147, 184
BssECI	CCNNGG	4 cut(s)	110, 358, 1102, 1129
BssMI	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
BssT1I	CCWWGG	3 cut(s)	110, 358, 1102
Bst2UI	CCWGG	1 cut(s)	1131
Bst4CI	ACNGT	3 cut(s)	161, 258, 640
Bst6I	CTCTTC	2 cut(s)	299, 596
BstAFI	CTTAAG	1 cut(s)	607
BstC8I	GCNNGC	2 cut(s)	998, 1002
BstDEI	CTNAG	3 cut(s)	578, 896, 983
BstDSI	CCRYGG	3 cut(s)	110, 358, 1102
BstENI	CCTNNNNNAGG	1 cut(s)	600
BstF5I	GGATG	4 cut(s)	13, 95, 120, 623
BstKTI	GATC	5 cut(s)	409, 584, 1128, 1185, 1282
BstMAI	GTCTC	1 cut(s)	906
BstMBI	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	3 cut(s)	318, 970, 1045
BstNI	CCWGG	1 cut(s)	1131
BstSCI	CCNGG	2 cut(s)	455, 1129
BstSFI	CTRYAG	3 cut(s)	687, 817, 945
BstSLI	GKGCMC	1 cut(s)	737
BstV1I	GCAGC	5 cut(s)	138, 333, 918, 921, 1057
BstV2I	GAAGAC	1 cut(s)	527
BstX2I	RGATCY	1 cut(s)	1182
BstYI	RGATCY	1 cut(s)	1182
Bsu36I	CCTNAGG	1 cut(s)	983
BsuRI	GGCC	2 cut(s)	188, 851
BtgI	CCRYGG	3 cut(s)	110, 358, 1102
BtgZI	GCGATG	1 cut(s)	151
BtsCI	GGATG	4 cut(s)	13, 95, 120, 623
BtsIMutI	CAGTG	3 cut(s)	636, 835, 1311
Cac8I	GCNNGC	2 cut(s)	998, 1002
CaiI	CAGNNNCTG	1 cut(s)	1136
Cfr10I	RCCGGY	2 cut(s)	147, 184
Cfr13I	GGNCC	1 cut(s)	515
Csp6I	GTAC	3 cut(s)	259, 381, 427
CspCI	CAANNNNNGTGG	2 cut(s)	894, 929
CviQI	GTAC	3 cut(s)	259, 381, 427
DdeI	CTNAG	3 cut(s)	578, 896, 983
DpnI	GATC	5 cut(s)	408, 583, 1127, 1184, 1281
DpnII	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
EaeI	YGGCCR	1 cut(s)	849
Eam1104I	CTCTTC	2 cut(s)	299, 596
EarI	CTCTTC	2 cut(s)	299, 596
Ecl136II	GAGCTC	1 cut(s)	200
Eco130I	CCWWGG	3 cut(s)	110, 358, 1102
Eco24I	GRGCYC	2 cut(s)	202, 1248
Eco31I	GGTCTC	1 cut(s)	906
Eco47I	GGWCC	1 cut(s)	515
Eco53kI	GAGCTC	1 cut(s)	200
Eco81I	CCTNAGG	1 cut(s)	983
EcoICRI	GAGCTC	1 cut(s)	200
EcoNI	CCTNNNNNAGG	1 cut(s)	600
EcoRII	CCWGG	1 cut(s)	1129
EcoT14I	CCWWGG	3 cut(s)	110, 358, 1102
EcoT38I	GRGCYC	2 cut(s)	202, 1248
ErhI	CCWWGG	3 cut(s)	110, 358, 1102
FalI	AAGNNNNNCTT	2 cut(s)	384, 416
FauNDI	CATATG	1 cut(s)	667
FbaI	TGATCA	1 cut(s)	1279
Fnu4HI	GCNGC	7 cut(s)	152, 280, 322, 852, 932, 935, 1046
FokI	GGATG	3 cut(s)	82, 127, 610
FriOI	GRGCYC	2 cut(s)	202, 1248
Fsp4HI	GCNGC	7 cut(s)	152, 280, 322, 852, 932, 935, 1046
FspBI	CTAG	2 cut(s)	57, 105
GluI	GCNGC	7 cut(s)	152, 280, 322, 852, 932, 935, 1046
GsaI	CCCAGC	1 cut(s)	1212
GsuI	CTGGAG	1 cut(s)	666
HaeIII	GGCC	2 cut(s)	188, 851
HapII	CCGG	4 cut(s)	148, 185, 456, 1165
HincII	GTYRAC	2 cut(s)	952, 1287
HindII	GTYRAC	2 cut(s)	952, 1287
HindIII	AAGCTT	2 cut(s)	6, 907
HinfI	GANTC	3 cut(s)	523, 559, 770
HpaI	GTTAAC	1 cut(s)	952
HpaII	CCGG	4 cut(s)	148, 185, 456, 1165
HphI	GGTGA	3 cut(s)	16, 374, 1232
Hpy166II	GTNNAC	3 cut(s)	65, 952, 1287
Hpy188I	TCNGA	5 cut(s)	505, 519, 558, 897, 1138
Hpy188III	TCNNGA	3 cut(s)	251, 316, 1277
Hpy8I	GTNNAC	3 cut(s)	65, 952, 1287
HpyAV	CCTTC	6 cut(s)	43, 55, 386, 606, 630, 748
HpyCH4III	ACNGT	3 cut(s)	161, 258, 640
HpyF10VI	GCNNNNNNNGC	3 cut(s)	318, 970, 1045
HpyF3I	CTNAG	3 cut(s)	578, 896, 983
KpnI	GGTACC	1 cut(s)	430
Ksp22I	TGATCA	1 cut(s)	1279
KspAI	GTTAAC	1 cut(s)	952
Kzo9I	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
LguI	GCTCTTC	1 cut(s)	596
LmnI	GCTCC	2 cut(s)	144, 883
Lsp1109I	GCAGC	5 cut(s)	138, 333, 918, 921, 1057
LweI	GCATC	1 cut(s)	901
MaeI	CTAG	2 cut(s)	57, 105
MaeIII	GTNAC	3 cut(s)	362, 634, 1264
MalI	GATC	5 cut(s)	408, 583, 1127, 1184, 1281
MboI	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
MboII	GAAGA	7 cut(s)	167, 286, 289, 465, 532, 583, 735
MfeI	CAATTG	2 cut(s)	543, 563
MflI	RGATCY	1 cut(s)	1182
MhlI	GDGCHC	3 cut(s)	202, 737, 1248
MluCI	AATT	7 cut(s)	543, 563, 739, 763, 800, 1058, 1293
MlyI	GAGTC	2 cut(s)	517, 553
MmeI	TCCRAC	2 cut(s)	264, 596
MroXI	GAANNNNTTC	1 cut(s)	9
MseI	TTAA	4 cut(s)	488, 608, 873, 951
MslI	CAYNNNNRTG	1 cut(s)	705
MspCI	CTTAAG	1 cut(s)	607
MspI	CCGG	4 cut(s)	148, 185, 456, 1165
MspR9I	CCNGG	2 cut(s)	457, 1131
MunI	CAATTG	2 cut(s)	543, 563
Mva1269I	GAATGC	1 cut(s)	1041
MvaI	CCWGG	1 cut(s)	1131
MwoI	GCNNNNNNNGC	3 cut(s)	318, 970, 1045
NciI	CCSGG	1 cut(s)	457
NcoI	CCATGG	3 cut(s)	110, 358, 1102
NdeI	CATATG	1 cut(s)	667
NdeII	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
NlaIV	GGNNCC	5 cut(s)	290, 428, 736, 879, 1184
NmuCI	GTSAC	3 cut(s)	362, 634, 1264
PciSI	GCTCTTC	1 cut(s)	596
PctI	GAATGC	1 cut(s)	1041
PdmI	GAANNNNTTC	1 cut(s)	9
PfeI	GAWTC	1 cut(s)	770
PflFI	GACNNNGTC	1 cut(s)	161
PflMI	CCANNNNNTGG	1 cut(s)	548
PkrI	GCNGC	7 cut(s)	153, 281, 323, 853, 933, 936, 1047
PleI	GAGTC	2 cut(s)	517, 553
PpsI	GAGTC	2 cut(s)	517, 553
Psp124BI	GAGCTC	1 cut(s)	202
Psp6I	CCWGG	1 cut(s)	1129
PspFI	CCCAGC	1 cut(s)	1208
PspGI	CCWGG	1 cut(s)	1129
PspN4I	GGNNCC	5 cut(s)	290, 428, 736, 879, 1184
PspPI	GGNCC	1 cut(s)	515
PstI	CTGCAG	2 cut(s)	691, 949
PstNI	CAGNNNCTG	1 cut(s)	1136
PsuI	RGATCY	1 cut(s)	1182
PsyI	GACNNNGTC	1 cut(s)	161
RsaI	GTAC	3 cut(s)	260, 382, 428
RsaNI	GTAC	3 cut(s)	259, 381, 427
RseI	CAYNNNNRTG	1 cut(s)	705
SacI	GAGCTC	1 cut(s)	202
SapI	GCTCTTC	1 cut(s)	596
SaqAI	TTAA	4 cut(s)	488, 608, 873, 951
SatI	GCNGC	7 cut(s)	152, 280, 322, 852, 932, 935, 1046
Sau3AI	GATC	5 cut(s)	406, 581, 1125, 1182, 1279
Sau96I	GGNCC	1 cut(s)	515
SchI	GAGTC	2 cut(s)	517, 553
ScrFI	CCNGG	2 cut(s)	457, 1131
SduI	GDGCHC	3 cut(s)	202, 737, 1248
SfaNI	GCATC	1 cut(s)	901
SfcI	CTRYAG	3 cut(s)	687, 817, 945
SinI	GGWCC	1 cut(s)	515
SmiMI	CAYNNNNRTG	1 cut(s)	705
SmlI	CTYRAG	2 cut(s)	314, 607
SmoI	CTYRAG	2 cut(s)	314, 607
SpeI	ACTAGT	1 cut(s)	56
Sse9I	AATT	7 cut(s)	543, 563, 739, 763, 800, 1058, 1293
SsiI	CCGC	3 cut(s)	280, 430, 852
SspI	AATATT	1 cut(s)	346
SspMI	CTAG	2 cut(s)	57, 105
SstI	GAGCTC	1 cut(s)	202
StyD4I	CCNGG	2 cut(s)	455, 1129
StyI	CCWWGG	3 cut(s)	110, 358, 1102
TaaI	ACNGT	3 cut(s)	161, 258, 640
TaqI	TCGA	3 cut(s)	156, 196, 202
TasI	AATT	7 cut(s)	543, 563, 739, 763, 800, 1058, 1293
TauI	GCSGC	2 cut(s)	282, 854
TfiI	GAWTC	1 cut(s)	770
Tru1I	TTAA	4 cut(s)	488, 608, 873, 951
Tru9I	TTAA	4 cut(s)	488, 608, 873, 951
TscAI	CASTG	3 cut(s)	643, 835, 1318
TseFI	GTSAC	3 cut(s)	362, 634, 1264
TseI	GCWGC	5 cut(s)	151, 321, 931, 934, 1045
Tsp45I	GTSAC	3 cut(s)	362, 634, 1264
TspRI	CASTG	3 cut(s)	643, 835, 1318
Tth111I	GACNNNGTC	1 cut(s)	161
Van91I	CCANNNNNTGG	1 cut(s)	548
Vha464I	CTTAAG	1 cut(s)	607
VpaK11BI	GGWCC	1 cut(s)	515
XagI	CCTNNNNNAGG	1 cut(s)	600
XapI	RAATTY	1 cut(s)	800
XmnI	GAANNNNTTC	1 cut(s)	9
XspI	CTAG	2 cut(s)	57, 105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.