Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0014215

Physical Location & Seq

Genomic Coordinates Forward (+)

24677801 .. 24680846

Gene Structure 3046 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g22270.10

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2481 bp

ATGTTTGTTCCCCATGTTTTCATTATGTTCCAATACTTCATAATACATATGTCATTCTATTTTGTAGTTTATGATGAAATCATATATATTTTAAGTATAAACAGACACTTATTTGCACAAAATATAATGAATTTACTTAAATCCGCTCCAGCCCGCCGCTTAACTATTTTGATGCTAAGGTTCCGACTTCTGTTGCCTTGCACTTTTCCTCTCTCTCTCTCTCGAAATTACACATGTCTCAGGAACCTCGAATCACCGCTCAGGAAAAGGAATACAAAAGAAACCAATTTGCAAAAGATAAAAACAGTCTATTTTGGGCACTACCTTTTAACGATTTTCACACATTCATTTTCTTTGTATATGCATCTCTCTTTATTTTTCGTCCATAAATCAAATCAAATAAAAGATAATCAAAGAATAAAAATATGTAGAGATGTGCAAAGAAAAACAAAATGTGTTTCTTCTATATATGAAGTGAAGTTTCAGCTCCCTTCTCCTAGCCTTCACACCCTTCACTCCAATCCCATGAAAGTTAACAACAAACCACCCTTCAGTTTCCAGTTTTATGTTCACATCTTCCAAAACAAAATTCGCACTGCTTGTGAAAAAAGTAAGCTTGCATGTAAGCACTTATTTCCCAAATTAGCACTCTTCAATTCCCTCCAATTTCTTTGCTTTCGTTCCACTCAACACGTACTAAATTTAGAGGCATATTTTACTTACAAGCCCTTGTCATCGTCCTACACAAAAATGGAAGCTTTTCATCCTTCTCTCACATTTCCCTTCTCCTACACCTTCACTACTACTACTAGTGCACTTGGTGGTGTCATTGCTGAAAACATCGACACATCATGTCCTAATCCATGGATGGATGCTAGGATATGGAGCAAGCTCCCCCACCGGCTCGTCGACCGTGTCATCGCCTTTCTCCCACCGCCGGCCTTTTTTCGCGCTCGATGTGTCTGCAAGAGGTGGTATGCCCTCTTGTTCTCCAACACCTTTCTCCAACTCTACCTCGAAGTGTCTCCGCGCCGTCATTGGTTCCTCTTCTTCAAGAACAAGAGCCTCAATGGAAGCTACATATACAGGAACAACAACAATGGAGGAAACAATGGTGACAGCAACAGGGGTGATACTAATTGTGAAGGTTATCTTTTTGATCCTTATGAAATTGCATGGTACCGCCTTTCGTTCGCTTTGCTCCCCTCCGGCCTTTCCCCTGCTTCATCTTCCGGCGGTTTAATCTGTTGGATTTCGGACGAGGCTGGCCCGAAGACTCTCATTCTATGCAACCCAATTGTTGGTAATGTGTCTCAGTTGCCTCCTACTCTAAGATCAAGACTCTTCCCTTCCATAGGGTTGAGTGTCAGCCCTACATCCATTGATGTAACTGTTGCTGGAGACGACTTGATCTCTCCATACGCTGTGAAAAACTTGACTGCCGAGAGCTTCCATATCGACGCAGGTGGGTTTTTTTCGCTGTGGGGAATCAATTCTTCCCTCCCAAGGCTCTGCAGTTTCGAATCAGGTCAAATGGTTAATGTTCAAGGAAGATTCTACTGCATGAACTACAGCCCTTTCAGTGTCTTAGCCTATGACGTGGCAGCAAACAATTGGTGGAAGATTCAGGCCCCCATGAGAAGGTTCCTGAGGTCTCCAAGCTTGGTGGAGAGCAGGGGGAAGCTGCTTTTAGTTGCTGCAGTTGAGAAGAGCAAGCTCAACGTGCCGAAAAGCTTGAGGATTTGGGGGTTGCAGGCTTGTGGAACAACTTGGGTTGAGATTGATAGGATGCCTCAGCAGCTCTATGTGCAATTTACTGACCTGGAGAATGGGAGTGGGTTCCAGTGTGTTGGACATGGAGAGTTCATCACCATCATGATTCTGGGTTCGGACAAGGCGTTGCTGTTCGATATGTGCAGGAAGAGGTGGCAGTGGATTCCACCGTGCCCTTATGTTCATGCTGGGGTTGATGGGGACTTGCATGGCTTTGCTTATGAACCCCGGCTCGCTACGCCGGTCACTGGGCTGCTTGATCAGCTGACTACAATTCCGTTCCAGGCTTTCAGTGCCGTAGGAAAAGCAGCGGTTGTGATGTGGGAGTTGTTTGAAGTAATCTGCTATATAGTCTGCATAACTATTGTGATAAATGTAGTAATATTTCCTGGAATTAACTACAAGATTGAAGCCAAGTTTTTTTTTTTTTTTTCTTATTATTATTATTTTTTTATTGCAACCGATATTTACACTAGGAACGAAGTATCAGACTCACCATTTCTGAAAGTCGAAGTTAAGGCCTTTCATTTAGAAATAGAGAAAAATACCACTTTAGTTTGTGCCGTTTCCGAGATCTATCATATTTTTTCCGTTCTTAAGCTGCTGTTTCAACTGTTAATCAAATTGATCAACAAGATTTCGAACCATGAAACCAATGATTTGTTGAGTATAAAAACTGACAGTCAAATACATATACGCGATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0006355 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009987 GO:0010468 GO:0010498 GO:0010556 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031146 GO:0031323 GO:0031326 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051603 GO:0060255 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box

Protein Analysis

827

Amino Acids

94.99

Weight (kDa)

9.22

Isoelectric Point (pI)

47.0

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30950
fragaria_vesca	FvH4_3g03990 FvH4_3g12960
malus_domestica	MD05G1246800.v1.1 MD10G1227400.v1.1
prunus_persica	Prupe.4G117600_v2.0.a1 Prupe.4G117800_v2.0.a1
pyrus_communis	pycom05g22270
rosa_chinensis	RchiOBHm_Chr5g0021071
rosa_laevigata	RLG00000032581
rosa_multiflora	Rmu_ssc0000244.1_g000018
rosa_roxburghii	Rroxscaffold_1G00057390
rosa_rugosa	Rorug05G0064100
rosa_samantha	Rh5BG153400 Rh5CG166300 Rh5DG153000
rosa_wichuraiana	Rw5G013660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1454
Acc36I	ACCTGC	1 cut(s)	1454
Acc65I	GGTACC	1 cut(s)	1179
AccB1I	GGYRCC	1 cut(s)	1179
AccB7I	CCANNNNNTGG	1 cut(s)	1301
AccBSI	CCGCTC	2 cut(s)	146, 259
AccI	GTMKAC	1 cut(s)	909
AccII	CGCG	3 cut(s)	951, 1030, 2472
AciI	CCGC	9 cut(s)	144, 154, 157, 257, 935, 1028, 1183, 1236, 2084
AclWI	GGATC	1 cut(s)	1154
AcsI	RAATTY	3 cut(s)	130, 588, 700
AcuI	CTGAAG	1 cut(s)	535
AdeI	CACNNNGTG	1 cut(s)	821
AfaI	GTAC	2 cut(s)	696, 1181
AfiI	CCNNNNNNNGG	6 cut(s)	937, 1301, 1355, 1506, 1641, 2021
AflII	CTTAAG	1 cut(s)	2369
AflIII	ACRYGT	2 cut(s)	233, 691
AgsI	TTSAA	6 cut(s)	655, 1054, 1547, 2108, 2183, 2384
AhlI	ACTAGT	1 cut(s)	809
AjiI	CACGTC	1 cut(s)	1600
AjnI	CCWGG	3 cut(s)	1821, 2055, 2161
AjuI	GAANNNNNNNTTGG	2 cut(s)	573, 605
Alw21I	GWGCWC	1 cut(s)	817
Alw26I	GTCTC	5 cut(s)	242, 1029, 1317, 1395, 1659
Alw44I	GTGCAC	1 cut(s)	813
AlwI	GGATC	1 cut(s)	1154
AoxI	GGCC	5 cut(s)	939, 1210, 1267, 1629, 2292
ApaLI	GTGCAC	1 cut(s)	813
ApeKI	GCWGC	7 cut(s)	1604, 1684, 1697, 1798, 2026, 2081, 2374
ApoI	RAATTY	3 cut(s)	130, 588, 700
Asp700I	GAANNNNTTC	2 cut(s)	759, 1492
Asp718I	GGTACC	1 cut(s)	1179
AspLEI	GCGC	2 cut(s)	953, 1032
AspS9I	GGNCC	2 cut(s)	1268, 1630
AsuC2I	CCSGG	1 cut(s)	2002
AsuHPI	GGTGA	5 cut(s)	246, 1127, 1142, 1861, 2259
AsuII	TTCGAA	2 cut(s)	1521, 2414
AxyI	CCTNAGG	1 cut(s)	1649
BaeGI	GKGCMC	3 cut(s)	321, 817, 1949
BanI	GGYRCC	1 cut(s)	1179
BbsI	GAAGAC	1 cut(s)	1280
Bbv12I	GWGCWC	1 cut(s)	817
BbvCI	CCTCAGC	1 cut(s)	1794
BbvI	GCAGC	7 cut(s)	1616, 1671, 1684, 1810, 2013, 2093, 2361
BccI	CCATC	3 cut(s)	862, 1880, 1964
BceAI	ACGGC	3 cut(s)	1017, 2054, 2321
BcgI	CGANNNNNNTGC	2 cut(s)	945, 979
BciT130I	CCWGG	3 cut(s)	1823, 2057, 2163
BclI	TGATCA	2 cut(s)	2032, 2400
BcnI	CCSGG	1 cut(s)	2002
BcoDI	GTCTC	5 cut(s)	242, 1029, 1317, 1395, 1659
BcuI	ACTAGT	1 cut(s)	809
BfaI	CTAG	4 cut(s)	498, 810, 876, 2247
BfmI	CTRYAG	3 cut(s)	1513, 1570, 1698
BfrI	CTTAAG	1 cut(s)	2369
BfuAI	ACCTGC	1 cut(s)	1454
BglII	AGATCT	1 cut(s)	2346
BisI	GCNGC	8 cut(s)	157, 1605, 1685, 1698, 1799, 2027, 2082, 2375
BlsI	GCNGC	8 cut(s)	158, 1606, 1686, 1699, 1800, 2028, 2083, 2376
Bme1390I	CCNGG	4 cut(s)	1823, 2002, 2057, 2163
BmgBI	CACGTC	1 cut(s)	1600
BmgT120I	GGNCC	2 cut(s)	1268, 1630
BmiI	GGNNCC	7 cut(s)	182, 245, 1043, 1181, 1632, 1646, 1841
BmrFI	CCNGG	4 cut(s)	1823, 2002, 2057, 2163
BmrI	ACTGGG	1 cut(s)	2031
BmsI	GCATC	4 cut(s)	162, 373, 862, 1779
BmuI	ACTGGG	1 cut(s)	2031
BpiI	GAAGAC	1 cut(s)	1280
BpmI	CTGGAG	3 cut(s)	132, 1419, 1844
Bpu10I	CCTNAGC	3 cut(s)	176, 260, 1794
Bpu14I	TTCGAA	2 cut(s)	1521, 2414
BpuEI	CTTGAG	1 cut(s)	1756
BpuMI	CCSGG	1 cut(s)	2002
BsaAI	YACGTR	1 cut(s)	694
BsaI	GGTCTC	1 cut(s)	1659
BsaJI	CCNNGG	3 cut(s)	863, 1505, 2000
Bsc4I	CCNNNNNNNGG	6 cut(s)	937, 1301, 1355, 1506, 1641, 2021
Bse118I	RCCGGY	3 cut(s)	900, 937, 2014
Bse1I	ACTGG	3 cut(s)	559, 1843, 2026
Bse21I	CCTNAGG	1 cut(s)	1649
Bse3DI	GCAATG	1 cut(s)	828
BseBI	CCWGG	3 cut(s)	1823, 2057, 2163
BseDI	CCNNGG	3 cut(s)	863, 1505, 2000
BseGI	GGATG	5 cut(s)	763, 873, 877, 1376, 1794
BseLI	CCNNNNNNNGG	6 cut(s)	937, 1301, 1355, 1506, 1641, 2021
BseMI	GCAATG	1 cut(s)	828
BseMII	CTCAG	5 cut(s)	253, 274, 1328, 1640, 1808
BseNI	ACTGG	3 cut(s)	559, 1843, 2026
BseSI	GKGCMC	3 cut(s)	321, 817, 1949
BseXI	GCAGC	7 cut(s)	1616, 1671, 1684, 1810, 2013, 2093, 2361
BseYI	CCCAGC	1 cut(s)	1961
BsgI	GTGCAG	1 cut(s)	1936
Bsh1236I	CGCG	3 cut(s)	951, 1030, 2472
Bsh1285I	CGRYCG	1 cut(s)	913
BshFI	GGCC	5 cut(s)	941, 1212, 1269, 1631, 2294
BshNI	GGYRCC	1 cut(s)	1179
BsiEI	CGRYCG	1 cut(s)	913
BsiHKAI	GWGCWC	1 cut(s)	817
BsiSI	CCGG	6 cut(s)	901, 938, 1209, 1233, 2002, 2015
BslFI	GGGAC	1 cut(s)	1988
BslI	CCNNNNNNNGG	6 cut(s)	937, 1301, 1355, 1506, 1641, 2021
BsmAI	GTCTC	5 cut(s)	242, 1029, 1317, 1395, 1659
BsmBI	CGTCTC	1 cut(s)	1395
BsmFI	GGGAC	1 cut(s)	1988
BsnI	GGCC	5 cut(s)	941, 1212, 1269, 1631, 2294
Bso31I	GGTCTC	1 cut(s)	1659
Bsp119I	TTCGAA	2 cut(s)	1521, 2414
Bsp1286I	GDGCHC	3 cut(s)	321, 817, 1949
Bsp143I	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
Bsp19I	CCATGG	1 cut(s)	863
BspACI	CCGC	9 cut(s)	144, 154, 157, 257, 935, 1028, 1183, 1236, 2084
BspANI	GGCC	5 cut(s)	941, 1212, 1269, 1631, 2294
BspCNI	CTCAG	5 cut(s)	252, 273, 1327, 1641, 1807
BspFNI	CGCG	3 cut(s)	951, 1030, 2472
BspHI	TCATGA	1 cut(s)	1875
BspLI	GGNNCC	7 cut(s)	182, 245, 1043, 1181, 1632, 1646, 1841
BspMAI	CTGCAG	2 cut(s)	1517, 1702
BspMI	ACCTGC	1 cut(s)	1454
BspPI	GGATC	1 cut(s)	1154
BspQI	GCTCTTC	1 cut(s)	1703
BspT104I	TTCGAA	2 cut(s)	1521, 2414
BspT107I	GGYRCC	1 cut(s)	1179
BspTI	CTTAAG	1 cut(s)	2369
BspTNI	GGTCTC	1 cut(s)	1659
BsrBI	CCGCTC	2 cut(s)	146, 259
BsrDI	GCAATG	1 cut(s)	828
BsrFI	RCCGGY	3 cut(s)	900, 937, 2014
BsrI	ACTGG	3 cut(s)	559, 1843, 2026
BssAI	RCCGGY	3 cut(s)	900, 937, 2014
BssECI	CCNNGG	3 cut(s)	863, 1505, 2000
BssMI	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
BssT1I	CCWWGG	2 cut(s)	863, 1505
Bst2UI	CCWGG	3 cut(s)	1823, 2057, 2163
Bst4CI	ACNGT	6 cut(s)	307, 914, 1393, 1944, 2388, 2456
Bst6I	CTCTTC	5 cut(s)	656, 1052, 1349, 1703, 1916
BstAFI	CTTAAG	1 cut(s)	2369
BstBAI	YACGTR	1 cut(s)	694
BstBI	TTCGAA	2 cut(s)	1521, 2414
BstC8I	GCNNGC	8 cut(s)	154, 618, 890, 939, 1267, 1715, 1755, 2007
BstDEI	CTNAG	8 cut(s)	176, 239, 260, 1314, 1331, 1588, 1649, 1794
BstDSI	CCRYGG	1 cut(s)	863
BstENI	CCTNNNNNAGG	1 cut(s)	1353
BstF5I	GGATG	5 cut(s)	763, 873, 877, 1376, 1794
BstFNI	CGCG	3 cut(s)	951, 1030, 2472
BstHHI	GCGC	2 cut(s)	953, 1032
BstKTI	GATC	6 cut(s)	1162, 1337, 1413, 2035, 2349, 2403
BstMAI	GTCTC	5 cut(s)	242, 1029, 1317, 1395, 1659
BstMBI	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
BstMCI	CGRYCG	1 cut(s)	913
BstMWI	GCNNNNNNNGC	6 cut(s)	1723, 1798, 1807, 2011, 2035, 2066
BstNI	CCWGG	3 cut(s)	1823, 2057, 2163
BstNSI	RCATGY	2 cut(s)	237, 624
BstSCI	CCNGG	4 cut(s)	1821, 2000, 2055, 2161
BstSFI	CTRYAG	3 cut(s)	1513, 1570, 1698
BstSLI	GKGCMC	3 cut(s)	321, 817, 1949
BstUI	CGCG	3 cut(s)	951, 1030, 2472
BstV1I	GCAGC	7 cut(s)	1616, 1671, 1684, 1810, 2013, 2093, 2361
BstV2I	GAAGAC	1 cut(s)	1280
BstX2I	RGATCY	1 cut(s)	2346
BstXI	CCANNNNNNTGG	1 cut(s)	1850
BstYI	RGATCY	1 cut(s)	2346
Bsu36I	CCTNAGG	1 cut(s)	1649
BsuRI	GGCC	5 cut(s)	941, 1212, 1269, 1631, 2294
BtgI	CCRYGG	1 cut(s)	863
BtgZI	GCGATG	1 cut(s)	904
BtrI	CACGTC	1 cut(s)	1600
BtsCI	GGATG	5 cut(s)	763, 873, 877, 1376, 1794
BtsI	GCAGTG	2 cut(s)	594, 1937
BtsIMutI	CAGTG	6 cut(s)	594, 1588, 1850, 1937, 2019, 2071
BveI	ACCTGC	1 cut(s)	1454
Cac8I	GCNNGC	8 cut(s)	154, 618, 890, 939, 1267, 1715, 1755, 2007
CciI	TCATGA	1 cut(s)	1875
CfoI	GCGC	2 cut(s)	953, 1032
Cfr10I	RCCGGY	3 cut(s)	900, 937, 2014
Cfr13I	GGNCC	2 cut(s)	1268, 1630
CseI	GACGC	1 cut(s)	1469
Csp6I	GTAC	2 cut(s)	695, 1180
CspCI	CAANNNNNGTGG	2 cut(s)	1647, 1682
CviQI	GTAC	2 cut(s)	695, 1180
DdeI	CTNAG	8 cut(s)	176, 239, 260, 1314, 1331, 1588, 1649, 1794
DpnI	GATC	6 cut(s)	1161, 1336, 1412, 2034, 2348, 2402
DpnII	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
DraIII	CACNNNGTG	1 cut(s)	821
Eam1104I	CTCTTC	5 cut(s)	656, 1052, 1349, 1703, 1916
EarI	CTCTTC	5 cut(s)	656, 1052, 1349, 1703, 1916
Eco130I	CCWWGG	2 cut(s)	863, 1505
Eco147I	AGGCCT	1 cut(s)	2294
Eco31I	GGTCTC	1 cut(s)	1659
Eco57I	CTGAAG	1 cut(s)	535
Eco81I	CCTNAGG	1 cut(s)	1649
EcoNI	CCTNNNNNAGG	1 cut(s)	1353
EcoO109I	RGGNCCY	1 cut(s)	1630
EcoRII	CCWGG	3 cut(s)	1821, 2055, 2161
EcoT14I	CCWWGG	2 cut(s)	863, 1505
EcoT22I	ATGCAT	1 cut(s)	366
ErhI	CCWWGG	2 cut(s)	863, 1505
Esp3I	CGTCTC	1 cut(s)	1395
FalI	AAGNNNNNCTT	2 cut(s)	1137, 1169
FaqI	GGGAC	1 cut(s)	1988
FauI	CCCGC	1 cut(s)	161
FauNDI	CATATG	1 cut(s)	48
FbaI	TGATCA	2 cut(s)	2032, 2400
FblI	GTMKAC	1 cut(s)	909
Fnu4HI	GCNGC	8 cut(s)	157, 1605, 1685, 1698, 1799, 2027, 2082, 2375
FokI	GGATG	5 cut(s)	750, 880, 884, 1363, 1801
Fsp4HI	GCNGC	8 cut(s)	157, 1605, 1685, 1698, 1799, 2027, 2082, 2375
FspBI	CTAG	4 cut(s)	498, 810, 876, 2247
GlaI	GCGC	2 cut(s)	952, 1031
GluI	GCNGC	8 cut(s)	157, 1605, 1685, 1698, 1799, 2027, 2082, 2375
GsaI	CCCAGC	1 cut(s)	1965
GsuI	CTGGAG	3 cut(s)	132, 1419, 1844
HaeIII	GGCC	5 cut(s)	941, 1212, 1269, 1631, 2294
HapII	CCGG	6 cut(s)	901, 938, 1209, 1233, 2002, 2015
HgaI	GACGC	1 cut(s)	1469
HhaI	GCGC	2 cut(s)	953, 1032
Hin6I	GCGC	2 cut(s)	951, 1030
HinP1I	GCGC	2 cut(s)	951, 1030
HincII	GTYRAC	2 cut(s)	535, 910
HindII	GTYRAC	2 cut(s)	535, 910
HindIII	AAGCTT	4 cut(s)	614, 756, 1660, 1732
HpaI	GTTAAC	1 cut(s)	535
HpaII	CCGG	6 cut(s)	901, 938, 1209, 1233, 2002, 2015
HphI	GGTGA	5 cut(s)	246, 1127, 1142, 1861, 2259
Hpy166II	GTNNAC	4 cut(s)	535, 571, 815, 910
Hpy188I	TCNGA	6 cut(s)	185, 1258, 1891, 2263, 2277, 2344
Hpy188III	TCNNGA	7 cut(s)	222, 241, 262, 1054, 1338, 1648, 1876
Hpy8I	GTNNAC	4 cut(s)	535, 571, 815, 910
Hpy99I	CGWCG	2 cut(s)	911, 1463
HpyCH4III	ACNGT	6 cut(s)	307, 914, 1393, 1944, 2388, 2456
HpyCH4IV	ACGT	3 cut(s)	693, 1599, 1722
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1723, 1798, 1807, 2011, 2035, 2066
HpyF3I	CTNAG	8 cut(s)	176, 239, 260, 1314, 1331, 1588, 1649, 1794
HpySE526I	ACGT	3 cut(s)	693, 1599, 1722
HspAI	GCGC	2 cut(s)	951, 1030
KpnI	GGTACC	1 cut(s)	1183
KroI	GCCGGC	1 cut(s)	937
KroNI	GCCGGC	1 cut(s)	939
Ksp22I	TGATCA	2 cut(s)	2032, 2400
KspAI	GTTAAC	1 cut(s)	535
Kzo9I	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
LguI	GCTCTTC	1 cut(s)	1703
LmnI	GCTCC	5 cut(s)	151, 492, 885, 897, 1206
Lsp1109I	GCAGC	7 cut(s)	1616, 1671, 1684, 1810, 2013, 2093, 2361
LweI	GCATC	4 cut(s)	162, 373, 862, 1779
MaeI	CTAG	4 cut(s)	498, 810, 876, 2247
MaeII	ACGT	3 cut(s)	693, 1599, 1722
MaeIII	GTNAC	3 cut(s)	1115, 1387, 2017
MalI	GATC	6 cut(s)	1161, 1336, 1412, 2034, 2348, 2402
MbiI	CCGCTC	2 cut(s)	146, 259
MboI	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
MfeI	CAATTG	2 cut(s)	1296, 1612
MflI	RGATCY	1 cut(s)	2346
MhlI	GDGCHC	3 cut(s)	321, 817, 1949
MlyI	GAGTC	3 cut(s)	1270, 1335, 2258
MmeI	TCCRAC	5 cut(s)	208, 1017, 1030, 1229, 1831
Mph1103I	ATGCAT	1 cut(s)	366
MroNI	GCCGGC	1 cut(s)	937
MroXI	GAANNNNTTC	2 cut(s)	759, 1492
MslI	CAYNNNNRTG	2 cut(s)	749, 1874
MspA1I	CMGCKG	2 cut(s)	2038, 2084
MspCI	CTTAAG	1 cut(s)	2369
MspI	CCGG	6 cut(s)	901, 938, 1209, 1233, 2002, 2015
MspR9I	CCNGG	4 cut(s)	1823, 2002, 2057, 2163
MunI	CAATTG	2 cut(s)	1296, 1612
MvaI	CCWGG	3 cut(s)	1823, 2057, 2163
MvnI	CGCG	3 cut(s)	951, 1030, 2472
MwoI	GCNNNNNNNGC	6 cut(s)	1723, 1798, 1807, 2011, 2035, 2066
NaeI	GCCGGC	1 cut(s)	939
NciI	CCSGG	1 cut(s)	2002
NcoI	CCATGG	1 cut(s)	863
NdeI	CATATG	1 cut(s)	48
NdeII	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
NgoMIV	GCCGGC	1 cut(s)	937
NlaIV	GGNNCC	7 cut(s)	182, 245, 1043, 1181, 1632, 1646, 1841
NmeAIII	GCCGAG	1 cut(s)	1468
NmuCI	GTSAC	2 cut(s)	1115, 2017
NsiI	ATGCAT	1 cut(s)	366
NspI	RCATGY	2 cut(s)	237, 624
NspV	TTCGAA	2 cut(s)	1521, 2414
PagI	TCATGA	1 cut(s)	1875
PaqCI	CACCTGC	1 cut(s)	1454
PceI	AGGCCT	1 cut(s)	2294
PciI	ACATGT	1 cut(s)	233
PciSI	GCTCTTC	1 cut(s)	1703
PcsI	WCGNNNNNNNCGW	1 cut(s)	1895
PdiI	GCCGGC	1 cut(s)	939
PdmI	GAANNNNTTC	2 cut(s)	759, 1492
PfeI	GAWTC	7 cut(s)	251, 1488, 1523, 1554, 1624, 1879, 1936
PflFI	GACNNNGTC	1 cut(s)	914
PflMI	CCANNNNNTGG	1 cut(s)	1301
PfoI	TCCNGGA	1 cut(s)	2161
PkrI	GCNGC	8 cut(s)	158, 1606, 1686, 1699, 1800, 2028, 2083, 2376
PleI	GAGTC	3 cut(s)	1270, 1335, 2258
PpsI	GAGTC	3 cut(s)	1270, 1335, 2258
Ppu21I	YACGTR	1 cut(s)	694
PscI	ACATGT	1 cut(s)	233
Psp6I	CCWGG	3 cut(s)	1821, 2055, 2161
PspFI	CCCAGC	1 cut(s)	1961
PspGI	CCWGG	3 cut(s)	1821, 2055, 2161
PspN4I	GGNNCC	7 cut(s)	182, 245, 1043, 1181, 1632, 1646, 1841
PspPI	GGNCC	2 cut(s)	1268, 1630
PstI	CTGCAG	2 cut(s)	1517, 1702
PsuI	RGATCY	1 cut(s)	2346
PsyI	GACNNNGTC	1 cut(s)	914
PvuII	CAGCTG	1 cut(s)	2038
RsaI	GTAC	2 cut(s)	696, 1181
RsaNI	GTAC	2 cut(s)	695, 1180
RseI	CAYNNNNRTG	2 cut(s)	749, 1874
SalI	GTCGAC	1 cut(s)	908
SapI	GCTCTTC	1 cut(s)	1703
SatI	GCNGC	8 cut(s)	157, 1605, 1685, 1698, 1799, 2027, 2082, 2375
Sau3AI	GATC	6 cut(s)	1159, 1334, 1410, 2032, 2346, 2400
Sau96I	GGNCC	2 cut(s)	1268, 1630
SchI	GAGTC	3 cut(s)	1270, 1335, 2258
ScrFI	CCNGG	4 cut(s)	1823, 2002, 2057, 2163
SduI	GDGCHC	3 cut(s)	321, 817, 1949
SfaNI	GCATC	4 cut(s)	162, 373, 862, 1779
SfcI	CTRYAG	3 cut(s)	1513, 1570, 1698
SfuI	TTCGAA	2 cut(s)	1521, 2414
SmiMI	CAYNNNNRTG	2 cut(s)	749, 1874
SmlI	CTYRAG	2 cut(s)	1735, 2369
SmoI	CTYRAG	2 cut(s)	1735, 2369
SpeI	ACTAGT	1 cut(s)	809
SseBI	AGGCCT	1 cut(s)	2294
SsiI	CCGC	9 cut(s)	144, 154, 157, 257, 935, 1028, 1183, 1236, 2084
SspI	AATATT	1 cut(s)	2157
SspMI	CTAG	4 cut(s)	498, 810, 876, 2247
StuI	AGGCCT	1 cut(s)	2294
StyD4I	CCNGG	4 cut(s)	1821, 2000, 2055, 2161
StyI	CCWWGG	2 cut(s)	863, 1505
TaaI	ACNGT	6 cut(s)	307, 914, 1393, 1944, 2388, 2456
TaiI	ACGT	3 cut(s)	696, 1602, 1725
TauI	GCSGC	1 cut(s)	159
TfiI	GAWTC	7 cut(s)	251, 1488, 1523, 1554, 1624, 1879, 1936
TscAI	CASTG	6 cut(s)	601, 1588, 1850, 1937, 2026, 2071
TseFI	GTSAC	2 cut(s)	1115, 2017
TseI	GCWGC	7 cut(s)	1604, 1684, 1697, 1798, 2026, 2081, 2374
Tsp45I	GTSAC	2 cut(s)	1115, 2017
TspGWI	ACGGA	2 cut(s)	2040, 2353
TspRI	CASTG	6 cut(s)	601, 1588, 1850, 1937, 2026, 2071
Tth111I	GACNNNGTC	1 cut(s)	914
Van91I	CCANNNNNTGG	1 cut(s)	1301
Vha464I	CTTAAG	1 cut(s)	2369
VneI	GTGCAC	1 cut(s)	813
XagI	CCTNNNNNAGG	1 cut(s)	1353
XapI	RAATTY	3 cut(s)	130, 588, 700
XceI	RCATGY	2 cut(s)	237, 624
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1879
XmiI	GTMKAC	1 cut(s)	909
XmnI	GAANNNNTTC	2 cut(s)	759, 1492
XspI	CTAG	4 cut(s)	498, 810, 876, 2247
Zsp2I	ATGCAT	1 cut(s)	366

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.