Prupe.4G251400_v2.0.a1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0010109
Physical Location & Seq
Forward (+)
16829036 .. 16832560
3525 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G251400.1

Sequence Viewer

Length: 1545 bp
ATGTCATCTGCAACAACAGTAACAGGAGGAGCAACAAGAAGACCAAAATGGCAGTACCCACCAGCACCACCAACCCCAAAAATTCTCCACTTTCCGCGTAGGCCTCGGCGGAGAGCATCCAAGAGCGTTCATGGCGGAAGACCCAATTCAGGGGAGGCCAAGAAGGACAACAAGGGCAAGCTGGAAGCTCTGTTTGACCAAGAACGAGTTTTTTCCAGGGTCGGTTTGCCCATTGTGCTTTTTGAGCATGGTGATGGGGAAAGTGAGTGCAGGAGAGAGAGGGTTAAAGAGGGTGAGGATGGTGGTGATGAAGTGGTGGAAGAGGAGAAGTGGAGATTTCAGGCTGAGATGTTGAGGGCAGAGTGCAATTTATTGAGGATGGAGAATGAGATTGCAGTTAAGAAGATGGAGAGGGTCAAGGTCAAGGTGGAGAGGACTCTTAAATCAGCTGTTCACACTCTTGTTTCTGGGAGAAAGAAGATTTGCGAAGGAAAGAATGCAAGTATGGTATTGGAGGAAAAACTTCAACACTTGGCAGAAAAGCTAGAGAAGTTGCAAAGGAATTTAGGAGTTAAGGATTCGGAGGTCCGAAACACTAGTAATTTTGATAAACAAGCGTATCTTCTTCAAAGAAAGCTACAGAAGTTCAGACGAACATCGGATGAGATATGTGTCAAGGAGATCCAAGAGATGGCAGAAGCAAGCTTTTCTATCAAAACAAGCCATAGAGTAAATGAGAACTTGCTTTCGAGTGGCAAAAGTAATGTAGACATTCTCAGAAGGAAAATGGAGGGATTGTCAAATGGGATGCTATTAGAGAGGATGAAGGAAGAGTATGGTTCAATGCTGTCTACCGCCGACTGTTCTGTTGCCAGTTCTGCCTCTTCTTCCCAGAGAATTGAATCCACCAATTTGTCTTCTTCTTTGATACAACAATTTTACAAGGAGAAAGAGTGTCGTGAAGAGAATGTGTGCTCAGGACGTTGCAAGGCAATAGTTCGAAGAATTGTAGAGCAAGTACGAATTGAGACAGAGCAATGGTCTCAGATGCAGGAGATGCTGGGGCAGGTGAAGGAGGAGATGGAAGAATTGCATGCCTCTCGAGACTTTTGGAAAGATCGAGCGCTAGATTCTGATTATCACATCCAATCCTTACAATCCGTTGTGCAAGAATGGAAACAAAAAGCTGTCTCATATGAAAGCAAAGCAAAGGAGTTGCAGGCACAAGTGTCTATACTCCATGGAGAGCTTGATAGGTTGAGGAAGGAAGCAAGCATAAGAGCAATGAAGGCCAATGGTTCACCACTTATACCCCGGGATCCACAGAACGAAATGGAAAAACACGTCCTGATTTGTCACTTGAAGGAAAATCATTGTCCCAAAGAAGGTGGCAGCAAGCAAAGAAAACTAGCCACATGTACCAACAGAGTGTTTGTTGCTCCAAAAAGATCACCCTTTCGAGACATCGGAAACTCCTCATTGCCGGTGAGGCAGAACAGCAAAGCAGTCTTCCCTTTGCATTGCCCTCTACCTTCCAAGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

515

Amino Acids

58.74

Weight (kDa)

9.38

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1057
Acc36I ACCTGC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 691
AccI GTMKAC 2 cut(s) 768, 851
AccII CGCG 1 cut(s) 97
AciI CCGC 4 cut(s) 95, 109, 135, 855
AclWI GGATC 3 cut(s) 676, 1313, 1326
AcsI RAATTY 2 cut(s) 81, 562
AfaI GTAC 3 cut(s) 56, 1020, 1420
AfeI AGCGCT 1 cut(s) 1125
AfiI CCNNNNNNNGG 4 cut(s) 149, 150, 691, 1385
AflIII ACRYGT 2 cut(s) 1342, 1415
AgsI TTSAA 5 cut(s) 527, 629, 843, 902, 1363
AhlI ACTAGT 1 cut(s) 596
AjiI CACGTC 1 cut(s) 1345
AjnI CCWGG 1 cut(s) 215
AluBI AGCT 8 cut(s) 181, 188, 449, 544, 637, 705, 1187, 1249
AluI AGCT 8 cut(s) 181, 188, 449, 544, 637, 705, 1187, 1249
Alw21I GWGCWC 1 cut(s) 977
Alw26I GTCTC 5 cut(s) 1022, 1047, 1098, 1195, 1455
AlwI GGATC 3 cut(s) 676, 1313, 1326
Ama87I CYCGRG 2 cut(s) 1101, 1314
Aor51HI AGCGCT 1 cut(s) 1125
AoxI GGCC 3 cut(s) 101, 156, 1290
ApeKI GCWGC 1 cut(s) 1392
ApoI RAATTY 2 cut(s) 81, 562
Asp700I GAANNNNTTC 1 cut(s) 522
AspLEI GCGC 1 cut(s) 1126
AspS9I GGNCC 1 cut(s) 586
AsuC2I CCSGG 2 cut(s) 1315, 1316
AsuHPI GGTGA 7 cut(s) 263, 305, 317, 1081, 1293, 1443, 1498
AsuII TTCGAA 1 cut(s) 1000
AvaI CYCGRG 2 cut(s) 1101, 1314
AvaII GGWCC 1 cut(s) 586
BaeI ACNNNNGTAYC 2 cut(s) 602, 635
BamHI GGATCC 1 cut(s) 1318
BbsI GAAGAC 4 cut(s) 46, 145, 909, 1501
Bbv12I GWGCWC 1 cut(s) 977
BbvI GCAGC 1 cut(s) 1404
BccI CCATC 6 cut(s) 248, 293, 373, 400, 685, 1075
BcgI CGANNNNNNTGC 2 cut(s) 1082, 1116
BciT130I CCWGG 1 cut(s) 217
BcnI CCSGG 2 cut(s) 1315, 1316
BcoDI GTCTC 5 cut(s) 1022, 1047, 1098, 1195, 1455
BcuI ACTAGT 1 cut(s) 596
BfaI CTAG 4 cut(s) 545, 597, 1127, 1409
BfmI CTRYAG 1 cut(s) 638
BfoI RGCGCY 1 cut(s) 1127
BfuAI ACCTGC 1 cut(s) 1057
BglI GCCNNNNNGGC 1 cut(s) 1489
BisI GCNGC 1 cut(s) 1393
BlsI GCNGC 1 cut(s) 1394
Bme1390I CCNGG 3 cut(s) 217, 1315, 1316
Bme18I GGWCC 1 cut(s) 586
BmeT110I CYCGRG 2 cut(s) 1101, 1314
BmgBI CACGTC 1 cut(s) 1345
BmgT120I GGNCC 1 cut(s) 586
BmiI GGNNCC 1 cut(s) 1320
BmrFI CCNGG 3 cut(s) 217, 1315, 1316
BmsI GCATC 4 cut(s) 125, 798, 1038, 1047
BpiI GAAGAC 4 cut(s) 46, 145, 909, 1501
Bpu10I CCTNAGC 1 cut(s) 976
Bpu14I TTCGAA 1 cut(s) 1000
BpuMI CCSGG 2 cut(s) 1315, 1316
BsaI GGTCTC 1 cut(s) 1047
BsaJI CCNNGG 5 cut(s) 104, 216, 1240, 1313, 1314
BsaXI ACNNNNNCTCC 4 cut(s) 782, 812, 938, 968
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 150, 691, 1385
Bse118I RCCGGY 1 cut(s) 1483
Bse1I ACTGG 1 cut(s) 873
Bse3DI GCAATG 4 cut(s) 1043, 1290, 1478, 1519
BseBI CCWGG 1 cut(s) 217
BseDI CCNNGG 5 cut(s) 104, 216, 1240, 1313, 1314
BseGI GGATG 7 cut(s) 116, 304, 384, 667, 813, 828, 1143
BseLI CCNNNNNNNGG 4 cut(s) 149, 150, 691, 1385
BseMI GCAATG 4 cut(s) 1043, 1290, 1478, 1519
BseMII CTCAG 4 cut(s) 336, 790, 990, 1058
BseNI ACTGG 1 cut(s) 873
BseRI GAGGAG 4 cut(s) 42, 338, 1091, 1465
BseXI GCAGC 1 cut(s) 1404
BseYI CCCAGC 1 cut(s) 1060
BsgI GTGCAG 1 cut(s) 289
Bsh1236I CGCG 1 cut(s) 97
BshFI GGCC 3 cut(s) 103, 158, 1292
BsiHKAI GWGCWC 1 cut(s) 977
BsiHKCI CYCGRG 2 cut(s) 1101, 1314
BsiSI CCGG 2 cut(s) 1315, 1484
BslFI GGGAC 1 cut(s) 1362
BslI CCNNNNNNNGG 4 cut(s) 149, 150, 691, 1385
BsmAI GTCTC 5 cut(s) 1022, 1047, 1098, 1195, 1455
BsmFI GGGAC 1 cut(s) 1362
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 3 cut(s) 103, 158, 1292
Bso31I GGTCTC 1 cut(s) 1047
BsoBI CYCGRG 2 cut(s) 1101, 1314
Bsp119I TTCGAA 1 cut(s) 1000
Bsp1286I GDGCHC 1 cut(s) 977
Bsp143I GATC 4 cut(s) 681, 1117, 1318, 1448
Bsp19I CCATGG 1 cut(s) 1240
BspACI CCGC 4 cut(s) 95, 109, 135, 855
BspANI GGCC 3 cut(s) 103, 158, 1292
BspCNI CTCAG 4 cut(s) 337, 789, 989, 1057
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 1320
BspMI ACCTGC 1 cut(s) 1057
BspPI GGATC 3 cut(s) 676, 1313, 1326
BspT104I TTCGAA 1 cut(s) 1000
BspTNI GGTCTC 1 cut(s) 1047
BsrDI GCAATG 4 cut(s) 1043, 1290, 1478, 1519
BsrFI RCCGGY 1 cut(s) 1483
BsrI ACTGG 1 cut(s) 873
BssAI RCCGGY 1 cut(s) 1483
BssECI CCNNGG 5 cut(s) 104, 216, 1240, 1313, 1314
BssMI GATC 4 cut(s) 681, 1117, 1318, 1448
BssT1I CCWWGG 1 cut(s) 1240
Bst2UI CCWGG 1 cut(s) 217
Bst4CI ACNGT 2 cut(s) 19, 863
Bst6I CTCTTC 4 cut(s) 315, 825, 889, 957
BstAPI GCANNNNNTGC 1 cut(s) 1057
BstBI TTCGAA 1 cut(s) 1000
BstC8I GCNNGC 6 cut(s) 179, 703, 1095, 1221, 1273, 1397
BstDEI CTNAG 4 cut(s) 345, 776, 976, 1044
BstDSI CCRYGG 1 cut(s) 1240
BstF5I GGATG 7 cut(s) 116, 304, 384, 667, 813, 828, 1143
BstFNI CGCG 1 cut(s) 97
BstH2I RGCGCY 1 cut(s) 1127
BstHHI GCGC 1 cut(s) 1126
BstKTI GATC 4 cut(s) 684, 1120, 1321, 1451
BstMAI GTCTC 5 cut(s) 1022, 1047, 1098, 1195, 1455
BstMBI GATC 4 cut(s) 681, 1117, 1318, 1448
BstMWI GCNNNNNNNGC 7 cut(s) 132, 235, 244, 878, 1057, 1289, 1489
BstNI CCWGG 1 cut(s) 217
BstNSI RCATGY 2 cut(s) 1097, 1419
BstSCI CCNGG 3 cut(s) 215, 1313, 1314
BstSFI CTRYAG 1 cut(s) 638
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 1404
BstV2I GAAGAC 4 cut(s) 46, 145, 909, 1501
BstX2I RGATCY 2 cut(s) 681, 1318
BstYI RGATCY 2 cut(s) 681, 1318
BsuRI GGCC 3 cut(s) 103, 158, 1292
BtgI CCRYGG 1 cut(s) 1240
BtrI CACGTC 1 cut(s) 1345
BtsCI GGATG 7 cut(s) 116, 304, 384, 667, 813, 828, 1143
BveI ACCTGC 1 cut(s) 1057
Cac8I GCNNGC 6 cut(s) 179, 703, 1095, 1221, 1273, 1397
CfoI GCGC 1 cut(s) 1126
Cfr10I RCCGGY 1 cut(s) 1483
Cfr13I GGNCC 1 cut(s) 586
Cfr9I CCCGGG 1 cut(s) 1314
Csp6I GTAC 3 cut(s) 55, 1019, 1419
CspCI CAANNNNNGTGG 2 cut(s) 1369, 1404
CviAII CATG 5 cut(s) 131, 248, 1094, 1241, 1416
CviQI GTAC 3 cut(s) 55, 1019, 1419
DdeI CTNAG 4 cut(s) 345, 776, 976, 1044
DpnI GATC 4 cut(s) 683, 1119, 1320, 1450
DpnII GATC 4 cut(s) 681, 1117, 1318, 1448
Eam1104I CTCTTC 4 cut(s) 315, 825, 889, 957
EarI CTCTTC 4 cut(s) 315, 825, 889, 957
EciI GGCGGA 2 cut(s) 124, 150
Eco130I CCWWGG 1 cut(s) 1240
Eco147I AGGCCT 1 cut(s) 103
Eco31I GGTCTC 1 cut(s) 1047
Eco47I GGWCC 1 cut(s) 586
Eco47III AGCGCT 1 cut(s) 1125
Eco88I CYCGRG 2 cut(s) 1101, 1314
EcoRII CCWGG 1 cut(s) 215
EcoT14I CCWWGG 1 cut(s) 1240
ErhI CCWWGG 1 cut(s) 1240
FaeI CATG 5 cut(s) 134, 251, 1097, 1244, 1419
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 1 cut(s) 1362
FatI CATG 5 cut(s) 130, 247, 1093, 1240, 1415
FauNDI CATATG 1 cut(s) 1195
FblI GTMKAC 2 cut(s) 768, 851
Fnu4HI GCNGC 1 cut(s) 1393
FokI GGATG 7 cut(s) 103, 311, 391, 674, 820, 835, 1130
Fsp4HI GCNGC 1 cut(s) 1393
FspBI CTAG 4 cut(s) 545, 597, 1127, 1409
GlaI GCGC 1 cut(s) 1125
GluI GCNGC 1 cut(s) 1393
GsaI CCCAGC 1 cut(s) 1064
HaeII RGCGCY 1 cut(s) 1127
HaeIII GGCC 3 cut(s) 103, 158, 1292
HapII CCGG 2 cut(s) 1315, 1484
HhaI GCGC 1 cut(s) 1126
Hin1II CATG 5 cut(s) 134, 251, 1097, 1244, 1419
Hin6I GCGC 1 cut(s) 1124
HinP1I GCGC 1 cut(s) 1124
HindIII AAGCTT 1 cut(s) 703
HinfI GANTC 4 cut(s) 436, 578, 902, 1130
HpaII CCGG 2 cut(s) 1315, 1484
HphI GGTGA 7 cut(s) 263, 305, 317, 1081, 1293, 1443, 1498
Hpy166II GTNNAC 4 cut(s) 454, 769, 852, 1301
Hpy188I TCNGA 8 cut(s) 583, 590, 650, 661, 779, 1047, 1135, 1469
Hpy188III TCNNGA 6 cut(s) 959, 978, 1101, 1103, 1348, 1460
Hpy8I GTNNAC 4 cut(s) 454, 769, 852, 1301
HpyCH4III ACNGT 2 cut(s) 19, 863
HpyCH4IV ACGT 2 cut(s) 982, 1344
HpyF10VI GCNNNNNNNGC 7 cut(s) 132, 235, 244, 878, 1057, 1289, 1489
HpyF3I CTNAG 4 cut(s) 345, 776, 976, 1044
HpySE526I ACGT 2 cut(s) 982, 1344
Hsp92II CATG 5 cut(s) 134, 251, 1097, 1244, 1419
HspAI GCGC 1 cut(s) 1124
Kzo9I GATC 4 cut(s) 681, 1117, 1318, 1448
LmnI GCTCC 2 cut(s) 29, 1444
Lsp1109I GCAGC 1 cut(s) 1404
LweI GCATC 4 cut(s) 125, 798, 1038, 1047
MaeI CTAG 4 cut(s) 545, 597, 1127, 1409
MaeII ACGT 2 cut(s) 982, 1344
MaeIII GTNAC 2 cut(s) 19, 1355
MalI GATC 4 cut(s) 683, 1119, 1320, 1450
MboI GATC 4 cut(s) 681, 1117, 1318, 1448
MflI RGATCY 2 cut(s) 681, 1318
MhlI GDGCHC 1 cut(s) 977
MlyI GAGTC 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 4 cut(s) 285, 399, 441, 573
MslI CAYNNNNRTG 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 2 cut(s) 1315, 1484
MspR9I CCNGG 3 cut(s) 217, 1315, 1316
Mva1269I GAATGC 1 cut(s) 502
MvaI CCWGG 1 cut(s) 217
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 7 cut(s) 132, 235, 244, 878, 1057, 1289, 1489
NciI CCSGG 2 cut(s) 1315, 1316
NcoI CCATGG 1 cut(s) 1240
NdeI CATATG 1 cut(s) 1195
NdeII GATC 4 cut(s) 681, 1117, 1318, 1448
NlaIII CATG 5 cut(s) 134, 251, 1097, 1244, 1419
NlaIV GGNNCC 1 cut(s) 1320
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 1 cut(s) 1355
NspI RCATGY 2 cut(s) 1097, 1419
NspV TTCGAA 1 cut(s) 1000
PaeI GCATGC 1 cut(s) 1097
PaeR7I CTCGAG 1 cut(s) 1101
PaqCI CACCTGC 1 cut(s) 1057
PceI AGGCCT 1 cut(s) 103
PciI ACATGT 1 cut(s) 1415
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 3 cut(s) 578, 902, 1130
PflMI CCANNNNNTGG 1 cut(s) 691
PkrI GCNGC 1 cut(s) 1394
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PscI ACATGT 1 cut(s) 1415
Psp6I CCWGG 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 1060
PspGI CCWGG 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 1320
PspPI GGNCC 1 cut(s) 586
PsuI RGATCY 2 cut(s) 681, 1318
PvuII CAGCTG 1 cut(s) 449
RsaI GTAC 3 cut(s) 56, 1020, 1420
RsaNI GTAC 3 cut(s) 55, 1019, 1419
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 4 cut(s) 285, 399, 441, 573
SatI GCNGC 1 cut(s) 1393
Sau3AI GATC 4 cut(s) 681, 1117, 1318, 1448
Sau96I GGNCC 1 cut(s) 586
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 3 cut(s) 217, 1315, 1316
SduI GDGCHC 1 cut(s) 977
SfaNI GCATC 4 cut(s) 125, 798, 1038, 1047
SfcI CTRYAG 1 cut(s) 638
Sfr274I CTCGAG 1 cut(s) 1101
SfuI TTCGAA 1 cut(s) 1000
SinI GGWCC 1 cut(s) 586
SlaI CTCGAG 1 cut(s) 1101
SmaI CCCGGG 1 cut(s) 1316
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 1101
SmoI CTYRAG 1 cut(s) 1101
SpeI ACTAGT 1 cut(s) 596
SphI GCATGC 1 cut(s) 1097
SseBI AGGCCT 1 cut(s) 103
SsiI CCGC 4 cut(s) 95, 109, 135, 855
SspMI CTAG 4 cut(s) 545, 597, 1127, 1409
StuI AGGCCT 1 cut(s) 103
StyD4I CCNGG 3 cut(s) 215, 1313, 1314
StyI CCWWGG 1 cut(s) 1240
TaaI ACNGT 2 cut(s) 19, 863
TaiI ACGT 2 cut(s) 985, 1347
TaqI TCGA 5 cut(s) 749, 1000, 1102, 1120, 1459
TfiI GAWTC 3 cut(s) 578, 902, 1130
Tru1I TTAA 4 cut(s) 285, 399, 441, 573
Tru9I TTAA 4 cut(s) 285, 399, 441, 573
TseFI GTSAC 1 cut(s) 1355
TseI GCWGC 1 cut(s) 1392
Tsp45I GTSAC 1 cut(s) 1355
TspDTI ATGAA 5 cut(s) 119, 324, 839, 1212, 1301
TspGWI ACGGA 1 cut(s) 1150
TspMI CCCGGG 1 cut(s) 1314
Van91I CCANNNNNTGG 1 cut(s) 691
VpaK11BI GGWCC 1 cut(s) 586
XapI RAATTY 2 cut(s) 81, 562
XceI RCATGY 2 cut(s) 1097, 1419
XhoI CTCGAG 1 cut(s) 1101
XmaI CCCGGG 1 cut(s) 1314
XmiI GTMKAC 2 cut(s) 768, 851
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 4 cut(s) 545, 597, 1127, 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.