Prupe.5G216400_v2.0.a1

Protein CHUP1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0017304
Physical Location & Seq
Forward (+)
16975115 .. 16977696
2582 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G216400.1

Sequence Viewer

Length: 1710 bp
ATGATAATCAAAATAAGCTTTCTGGCTGTGGCTTCAATTGCAGCATATGCAGTTTCACACAAAAACAGCAACTACACCAGGAAGCAACTAGACTCTGCCGCCATCAAGCCTCCGGAGAATGATGGCTCAAACTTCCAACAGCAACAGAACGAAGGAGAGATGGAAGATGAGGAGGAGGCGGCTGAAATATTTAGAAGCAAACAAATGAACAGTCTGAGTGAAATAGAGCTCTTGGTAAAGGAGCTGCAGCAGAGGAAAGTGAGGCTTGAAAAGAAGCTGCTAGAATTATGTGTTCTGAAAGAAGAGCAATCGTACATGGTTCACTTGCAAAGGCATATGGAGGACAAGTCTGCAGAGATTGGCATGCTTAACGTCACCATTGCTTCTTTGCAGGCTGAGAGGAAGGTTCTTCAGGAAGATGCCAAGCAGTGTGTTTTGGCAAAGAAGCAGCTGGAGATGGCAAAGAAGATGATAGCAGAAATGCAGAAGAAGATGGATTCTAATGAGAGCAGCATTATGAAGGGAAGGCTGTTGGTGATGGAAGAACAAGTTTCCAGCTTTCCCAAGGATGAAATCTCAGTCAGAGATGCCATAGCTGAGAAGAAGCTCAAAGCTGTTAAAAATGTTGAGTTGGAAGTTTTAAAGATGAAGAGGAGAAACAAAGAGCTTGAGCTAGAAAAGAGGGAATTGGCAGTCAAGTTGGTCTCTGCTGAAGCCAGAATAAGTACCCTGTCCAGCATGACTGAGAGTGAAACCGTTGCCAAGGTTGAAGAAGAAATAAGCAGGTTGAGGCATACCAATGCGGAACTCTCAAGGCAATTTGAAAGGCTGCAGAAAAACAGATTTGAAATGGTCCAAGAACGTGTGTACCAGCGCTGGCTCTACACTTGCTTGAGATTTGAAACCCAAATCCACAACAGTTCAAGCCATAAATCTCATGAGAAAAATGAACCATTGATATCATCAAATCCCAGTTACTATAGCCCTTCTTCTCACACATCTTCAACTAGTTTGAGCGATGAAATTGAGACTACCACCATTGACAGCTCCTCTTCAAGTAGCCAAAGAAGTATCAACAAGAAATCTGGTTTCATACATAGAATTAAGAGGTGGGGAAGAAGCAATGATGAGTCAGATGCCATTTCATTATCAGATAGGTCTTCTGGAGGAAACTCCCTGAGCAAGAAAGGTCTGGTTCGCAGGCTTTCCACATCAATGGTTCCGGTGAAGAAGACTTCCATTCTGAGAAACAAAGGTGACAGTGGAACCAGTTCTTCTTCTCCATCCTCCAAGACACCAACAAGTTCATCTAGAGGGAGGAGAAGAGTCTCATTTAGTGATTCAGTCAGATCAACAACCGATCAATACTCTATGCCAAAGCCATTTGACCATCAGAGGGTATTAATGAATTCAACCCAAAATTACAACAAGATTGAATTTGAGGGAGAGACACAAGAAGAAGACCCTTCATCACCAGAAGTGAGTGTTCCTGCAACAAATCCCACATTGACCAAAGATGACACCAACATTTCTGAAATTGCTGACACAGAAGTGGGGTTGAACAAAAAGACCATGCCTTTGCCTTGTGTGGATTTTGTTGCTGAGAAGGAGAACAAGGTGGATACAAATAATGTTGTGGGTTTTGTTGCTGCATTATTTTTGCTCCTCTTCATCTTAGTTGCCCGTCTTACATATTATACAGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

570

Amino Acids

64.38

Weight (kDa)

8.54

Isoelectric Point (pI)

64.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017304)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12771
malus_domestica MD06G1210200.v1.1
prunus_persica Prupe.5G216400_v2.0.a1
pyrus_communis pycom06g18790
rosa_chinensis RchiOBHm_Chr7g0184451
rosa_laevigata RLG00000004974
rosa_multiflora Rmu_sc0001557.1_g000015
rosa_roxburghii Rroxscaffold_3G00269600
rosa_samantha Rh7AG079800 Rh7BG067400 Rh7CG067700 Rh7DG067400
rosa_wichuraiana Rw7G005550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 774
AccIII TCCGGA 1 cut(s) 112
AciI CCGC 3 cut(s) 99, 179, 803
AcsI RAATTY 2 cut(s) 1408, 1436
AcuI CTGAAG 2 cut(s) 395, 732
AfaI GTAC 3 cut(s) 314, 727, 869
AfeI AGCGCT 1 cut(s) 875
AfiI CCNNNNNNNGG 1 cut(s) 1396
AflIII ACRYGT 1 cut(s) 862
AhlI ACTAGT 1 cut(s) 1007
AjnI CCWGG 1 cut(s) 77
AleI CACNNNNGTG 1 cut(s) 1550
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 4 cut(s) 709, 1022, 1333, 1442
Aor13HI TCCGGA 1 cut(s) 112
Aor51HI AGCGCT 1 cut(s) 875
ApeKI GCWGC 8 cut(s) 41, 244, 247, 277, 448, 510, 829, 1649
ApoI RAATTY 2 cut(s) 1408, 1436
ArsI GACNNNNNNTTYG 4 cut(s) 196, 228, 1561, 1593
AseI ATTAAT 1 cut(s) 1403
Asp700I GAANNNNTTC 1 cut(s) 1270
AspLEI GCGC 1 cut(s) 876
AspS9I GGNCC 1 cut(s) 853
AsuHPI GGTGA 5 cut(s) 367, 547, 1237, 1268, 1464
AsuNHI GCTAGC 1 cut(s) 1703
AvaII GGWCC 1 cut(s) 853
BaeI ACNNNNGTAYC 2 cut(s) 851, 884
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 3 cut(s) 1152, 1238, 1467
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 8 cut(s) 53, 231, 259, 264, 460, 522, 816, 1636
BccI CCATC 8 cut(s) 110, 116, 154, 451, 487, 532, 1291, 1398
BciT130I CCWGG 1 cut(s) 79
BciVI GTATCC 1 cut(s) 1615
BcoDI GTCTC 4 cut(s) 709, 1022, 1333, 1442
BcuI ACTAGT 1 cut(s) 1007
BfaI CTAG 7 cut(s) 89, 281, 674, 1008, 1311, 1704, 1708
BfmI CTRYAG 4 cut(s) 245, 351, 830, 979
BfoI RGCGCY 1 cut(s) 877
BfuAI ACCTGC 1 cut(s) 774
BfuI GTATCC 1 cut(s) 1615
Bme1390I CCNGG 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 853
BmgT120I GGNCC 1 cut(s) 853
BmiI GGNNCC 2 cut(s) 1221, 1267
BmrFI CCNGG 1 cut(s) 79
BmrI ACTGGG 1 cut(s) 966
BmsI GCATC 3 cut(s) 409, 577, 1126
BmtI GCTAGC 1 cut(s) 1707
BmuI ACTGGG 1 cut(s) 966
BpiI GAAGAC 3 cut(s) 1152, 1238, 1467
BpmI CTGGAG 2 cut(s) 473, 1185
Bpu10I CCTNAGC 1 cut(s) 1178
BpuEI CTTGAG 3 cut(s) 689, 796, 913
BsaI GGTCTC 1 cut(s) 709
BsaJI CCNNGG 2 cut(s) 564, 762
BsaWI WCCGGW 2 cut(s) 112, 1222
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 1396
Bse1I ACTGG 2 cut(s) 972, 1269
Bse3DI GCAATG 2 cut(s) 378, 1129
BseAI TCCGGA 1 cut(s) 112
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 2 cut(s) 564, 762
BseGI GGATG 2 cut(s) 574, 1283
BseLI CCNNNNNNNGG 1 cut(s) 1396
BseMI GCAATG 2 cut(s) 378, 1129
BseMII CTCAG 8 cut(s) 206, 387, 588, 591, 735, 1169, 1235, 1593
BseNI ACTGG 2 cut(s) 972, 1269
BseRI GAGGAG 6 cut(s) 185, 188, 667, 1039, 1333, 1655
BseXI GCAGC 8 cut(s) 53, 231, 259, 264, 460, 522, 816, 1636
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 113, 1223
BslI CCNNNNNNNGG 1 cut(s) 1396
BsmAI GTCTC 4 cut(s) 709, 1022, 1333, 1442
Bso31I GGTCTC 1 cut(s) 709
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 112
Bsp143I GATC 2 cut(s) 1349, 1360
BspACI CCGC 3 cut(s) 99, 179, 803
BspCNI CTCAG 8 cut(s) 207, 388, 589, 590, 736, 1170, 1236, 1594
BspEI TCCGGA 1 cut(s) 112
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 1221, 1267
BspMAI CTGCAG 3 cut(s) 249, 355, 834
BspMI ACCTGC 1 cut(s) 774
BspOI GCTAGC 1 cut(s) 1707
BspQI GCTCTTC 1 cut(s) 297
BspTNI GGTCTC 1 cut(s) 709
BsrDI GCAATG 2 cut(s) 378, 1129
BsrI ACTGG 2 cut(s) 972, 1269
BssECI CCNNGG 2 cut(s) 564, 762
BssMI GATC 2 cut(s) 1349, 1360
BssT1I CCWWGG 2 cut(s) 564, 762
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 4 cut(s) 212, 757, 920, 1262
Bst6I CTCTTC 5 cut(s) 297, 644, 1057, 1318, 1673
BstAPI GCANNNNNTGC 1 cut(s) 47
BstC8I GCNNGC 5 cut(s) 365, 393, 878, 1202, 1705
BstDEI CTNAG 9 cut(s) 215, 396, 577, 597, 744, 1178, 1244, 1602, 1675
BstF5I GGATG 2 cut(s) 574, 1283
BstH2I RGCGCY 1 cut(s) 877
BstHHI GCGC 1 cut(s) 876
BstKTI GATC 2 cut(s) 1352, 1363
BstMAI GTCTC 4 cut(s) 709, 1022, 1333, 1442
BstMBI GATC 2 cut(s) 1349, 1360
BstMWI GCNNNNNNNGC 2 cut(s) 38, 47
BstNI CCWGG 1 cut(s) 79
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 77
BstSFI CTRYAG 4 cut(s) 245, 351, 830, 979
BstV1I GCAGC 8 cut(s) 53, 231, 259, 264, 460, 522, 816, 1636
BstV2I GAAGAC 3 cut(s) 1152, 1238, 1467
BstXI CCANNNNNNTGG 1 cut(s) 1216
BsuI GTATCC 1 cut(s) 1615
BtgZI GCGATG 1 cut(s) 1032
BtsCI GGATG 2 cut(s) 574, 1283
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 2 cut(s) 434, 1267
BveI ACCTGC 1 cut(s) 774
Cac8I GCNNGC 5 cut(s) 365, 393, 878, 1202, 1705
CciI TCATGA 1 cut(s) 937
CfoI GCGC 1 cut(s) 876
Cfr13I GGNCC 1 cut(s) 853
Csp6I GTAC 3 cut(s) 313, 726, 868
CviAII CATG 5 cut(s) 316, 364, 739, 938, 1573
CviQI GTAC 3 cut(s) 313, 726, 868
DdeI CTNAG 9 cut(s) 215, 396, 577, 597, 744, 1178, 1244, 1602, 1675
DpnI GATC 2 cut(s) 1351, 1362
DpnII GATC 2 cut(s) 1349, 1360
DraI TTTAAA 1 cut(s) 642
Eam1104I CTCTTC 5 cut(s) 297, 644, 1057, 1318, 1673
EarI CTCTTC 5 cut(s) 297, 644, 1057, 1318, 1673
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 2 cut(s) 564, 762
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 709
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 1 cut(s) 853
Eco47III AGCGCT 1 cut(s) 875
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 395, 732
EcoICRI GAGCTC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 1408
EcoRII CCWGG 1 cut(s) 77
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 2 cut(s) 564, 762
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 2 cut(s) 564, 762
FaeI CATG 5 cut(s) 319, 367, 742, 941, 1576
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 5 cut(s) 315, 363, 738, 937, 1572
FauNDI CATATG 2 cut(s) 46, 336
FokI GGATG 2 cut(s) 581, 1270
FriOI GRGCYC 1 cut(s) 231
FspBI CTAG 7 cut(s) 89, 281, 674, 1008, 1311, 1704, 1708
GlaI GCGC 1 cut(s) 875
GsuI CTGGAG 2 cut(s) 473, 1185
HaeII RGCGCY 1 cut(s) 877
HapII CCGG 2 cut(s) 113, 1223
HhaI GCGC 1 cut(s) 876
Hin1II CATG 5 cut(s) 319, 367, 742, 941, 1576
Hin6I GCGC 1 cut(s) 874
HinP1I GCGC 1 cut(s) 874
HindIII AAGCTT 1 cut(s) 16
HinfI GANTC 5 cut(s) 92, 497, 1130, 1326, 1340
HpaII CCGG 2 cut(s) 113, 1223
HphI GGTGA 5 cut(s) 367, 547, 1237, 1268, 1464
Hpy166II GTNNAC 2 cut(s) 322, 868
Hpy188I TCNGA 9 cut(s) 216, 297, 584, 1135, 1153, 1245, 1349, 1395, 1534
Hpy188III TCNNGA 5 cut(s) 113, 413, 938, 1164, 1311
Hpy8I GTNNAC 2 cut(s) 322, 868
HpyAV CCTTC 7 cut(s) 146, 397, 514, 519, 996, 1476, 1600
HpyCH4III ACNGT 4 cut(s) 212, 757, 920, 1262
HpyCH4IV ACGT 2 cut(s) 372, 862
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 47
HpyF3I CTNAG 9 cut(s) 215, 396, 577, 597, 744, 1178, 1244, 1602, 1675
HpySE526I ACGT 2 cut(s) 372, 862
Hsp92II CATG 5 cut(s) 319, 367, 742, 941, 1576
HspAI GCGC 1 cut(s) 874
Kpn2I TCCGGA 1 cut(s) 112
Kzo9I GATC 2 cut(s) 1349, 1360
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 3 cut(s) 241, 1052, 1668
Lsp1109I GCAGC 8 cut(s) 53, 231, 259, 264, 460, 522, 816, 1636
LweI GCATC 3 cut(s) 409, 577, 1126
MaeI CTAG 7 cut(s) 89, 281, 674, 1008, 1311, 1704, 1708
MaeII ACGT 2 cut(s) 372, 862
MaeIII GTNAC 3 cut(s) 373, 974, 1256
MalI GATC 2 cut(s) 1351, 1362
MboI GATC 2 cut(s) 1349, 1360
MfeI CAATTG 1 cut(s) 36
MhlI GDGCHC 1 cut(s) 231
MlyI GAGTC 3 cut(s) 86, 1139, 1335
MmeI TCCRAC 2 cut(s) 160, 612
MroI TCCGGA 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 1270
MseI TTAA 5 cut(s) 369, 618, 641, 1104, 1403
MslI CAYNNNNRTG 3 cut(s) 798, 1214, 1550
MspA1I CMGCKG 1 cut(s) 451
MspI CCGG 2 cut(s) 113, 1223
MspR9I CCNGG 1 cut(s) 79
MunI CAATTG 1 cut(s) 36
MvaI CCWGG 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 38, 47
NdeI CATATG 2 cut(s) 46, 336
NdeII GATC 2 cut(s) 1349, 1360
NheI GCTAGC 1 cut(s) 1703
NlaIII CATG 5 cut(s) 319, 367, 742, 941, 1576
NlaIV GGNNCC 2 cut(s) 1221, 1267
NmuCI GTSAC 2 cut(s) 373, 1256
NspI RCATGY 1 cut(s) 367
OliI CACNNNNGTG 1 cut(s) 1550
PaeI GCATGC 1 cut(s) 367
PagI TCATGA 1 cut(s) 937
PciSI GCTCTTC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 1270
PfeI GAWTC 2 cut(s) 497, 1340
PleI GAGTC 3 cut(s) 86, 1138, 1334
PpsI GAGTC 3 cut(s) 86, 1138, 1334
PshBI ATTAAT 1 cut(s) 1403
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 1221, 1267
PspPI GGNCC 1 cut(s) 853
PstI CTGCAG 3 cut(s) 249, 355, 834
PvuII CAGCTG 1 cut(s) 451
RsaI GTAC 3 cut(s) 314, 727, 869
RsaNI GTAC 3 cut(s) 313, 726, 868
RseI CAYNNNNRTG 3 cut(s) 798, 1214, 1550
SacI GAGCTC 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 5 cut(s) 369, 618, 641, 1104, 1403
Sau3AI GATC 2 cut(s) 1349, 1360
Sau96I GGNCC 1 cut(s) 853
SchI GAGTC 3 cut(s) 86, 1139, 1335
ScrFI CCNGG 1 cut(s) 79
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 3 cut(s) 409, 577, 1126
SfcI CTRYAG 4 cut(s) 245, 351, 830, 979
SinI GGWCC 1 cut(s) 853
SmiMI CAYNNNNRTG 3 cut(s) 798, 1214, 1550
SmlI CTYRAG 3 cut(s) 668, 811, 892
SmoI CTYRAG 3 cut(s) 668, 811, 892
SpeI ACTAGT 1 cut(s) 1007
SphI GCATGC 1 cut(s) 367
SsiI CCGC 3 cut(s) 99, 179, 803
SspI AATATT 1 cut(s) 189
SspMI CTAG 7 cut(s) 89, 281, 674, 1008, 1311, 1704, 1708
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 2 cut(s) 564, 762
TaaI ACNGT 4 cut(s) 212, 757, 920, 1262
TaiI ACGT 2 cut(s) 375, 865
TauI GCSGC 2 cut(s) 101, 182
TfiI GAWTC 2 cut(s) 497, 1340
Tru1I TTAA 5 cut(s) 369, 618, 641, 1104, 1403
Tru9I TTAA 5 cut(s) 369, 618, 641, 1104, 1403
TscAI CASTG 2 cut(s) 434, 1267
TseFI GTSAC 2 cut(s) 373, 1256
TseI GCWGC 8 cut(s) 41, 244, 247, 277, 448, 510, 829, 1649
Tsp45I GTSAC 2 cut(s) 373, 1256
TspRI CASTG 2 cut(s) 434, 1267
VpaK11BI GGWCC 1 cut(s) 853
VspI ATTAAT 1 cut(s) 1403
XapI RAATTY 2 cut(s) 1408, 1436
XbaI TCTAGA 1 cut(s) 1310
XceI RCATGY 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 1270
XspI CTAG 7 cut(s) 89, 281, 674, 1008, 1311, 1704, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.