Prupe.6G063300_v2.0.a1

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011395
Physical Location & Seq
Forward (+)
4365490 .. 4367817
2328 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G063300.1

Sequence Viewer

Length: 801 bp
ATGTTGGAGCTCCGACTGGTTCAGGGGTCGCTGCTGAAGAAGGTTCTTGAGTCCATCAAGGACCTCGTCAACGACGCCAACTTCGATTGCTCCGCCACTGGCTTCTCCCTCCAGGCCATGGATTCCAGCCACGTGGCGCTGGTGGCGCTGCTTCTCAGATCCGAGGGCTTCGAGCACTACCGCTGCGACCGCAATATCTCCATGGGCATGAACCTCACCAACATGTCCAAGATGCTGAAATGCGCCGGAAATGATGACATCATCACTATCAAGGCTGACGATGGCAGCGATACTGTCACCTTCATGTTCGAAAGCCCCACGCAAGATAAAATTTCTGATTTTGAGATGAAGCTGATGGACATTGATAGTGAGCACCTTGGAATTCCGGAGGCAGAATACCATGCTATTGTTAAGATGCCTTCAGCTGAGTTTGCTAGGATATGTAAAGACCTCAGTGGCATTGGTGATACAGTTGTGATATCTGTGACCAAGGAAGGTGTGAAGTTCTCTACAAGAGGGGATATTGGCACTGCTAACATCGTCTGTAGGCAGAACACTACAGTGGACAAGCCTGAAGAAGCAACAGTTATTGATATGAATGAGCCAGTTTCATTGACATTTGCTCTGAGGTACATGAATTCCTTCACAAAAGCGACCACATTGTCGAACACAGTTACAATCAGCCTGTCTTCTGAACTTCCGGTTGTGGTTGAGTACAAGATTGCAGAGATGGGCTACATCAGGTTCTACTTGGCTCCTAAGATAGAAGAAGATGAAGATGAGACGAAGCCTGAAGTTTGA

Protein Analysis

267

Amino Acids

29.48

Weight (kDa)

4.63

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07370 AT2G29570
fragaria_vesca FvH4_3g37710 FvH4_6g24870
malus_domestica MD03G1078300.v1.1 MD11G1083000.v1.1 MD13G1227300.v1.1
prunus_persica Prupe.6G063300_v2.0.a1
pyrus_communis pycom03g06260 pycom11g07110
rosa_chinensis RchiOBHm_Chr5g0067981
rosa_laevigata RLG00000035949
rosa_multiflora Rmu_sc0000435.1_g000036
rosa_roxburghii Rroxscaffold_1G00012620 Rroxscaffold_1G00013330
rosa_rugosa Rorug05G0387000
rosa_samantha Rh5BG463400 Rh5CG485300 Rh5DG477400
rosa_wichuraiana Rw5G041640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 661
AccB7I CCANNNNNTGG 1 cut(s) 118
AccIII TCCGGA 1 cut(s) 385
AciI CCGC 3 cut(s) 93, 181, 190
AclWI GGATC 1 cut(s) 153
AcsI RAATTY 3 cut(s) 330, 381, 637
AcuI CTGAAG 3 cut(s) 56, 405, 594
AcvI CACGTG 1 cut(s) 133
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 632, 716
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 1 cut(s) 222
AjnI CCWGG 1 cut(s) 111
AleI CACNNNNGTG 1 cut(s) 560
AluBI AGCT 3 cut(s) 10, 352, 425
AluI AGCT 3 cut(s) 10, 352, 425
Alw21I GWGCWC 3 cut(s) 12, 177, 375
Alw26I GTCTC 1 cut(s) 776
AlwI GGATC 1 cut(s) 153
Aor13HI TCCGGA 1 cut(s) 385
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 4 cut(s) 31, 148, 183, 285
ApoI RAATTY 3 cut(s) 330, 381, 637
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp700I GAANNNNTTC 1 cut(s) 641
AspLEI GCGC 3 cut(s) 139, 148, 245
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 3 cut(s) 208, 289, 476
AsuII TTCGAA 1 cut(s) 309
AvaII GGWCC 1 cut(s) 61
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BanII GRGCYC 1 cut(s) 12
BbrPI CACGTG 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 681
Bbv12I GWGCWC 3 cut(s) 12, 177, 375
BbvI GCAGC 4 cut(s) 18, 135, 170, 297
BccI CCATC 4 cut(s) 62, 275, 349, 724
BciT130I CCWGG 1 cut(s) 113
BcoDI GTCTC 1 cut(s) 776
BfaI CTAG 1 cut(s) 435
BfmI CTRYAG 2 cut(s) 544, 558
BfoI RGCGCY 2 cut(s) 140, 149
BisI GCNGC 4 cut(s) 32, 149, 184, 286
BlsI GCNGC 4 cut(s) 33, 150, 185, 287
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 756
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 2 cut(s) 222, 405
BpiI GAAGAC 1 cut(s) 681
BpmI CTGGAG 1 cut(s) 95
Bpu14I TTCGAA 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 68
BsaAI YACGTR 1 cut(s) 133
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 5 cut(s) 117, 162, 201, 376, 489
BsaWI WCCGGW 2 cut(s) 385, 700
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse1I ACTGG 3 cut(s) 21, 103, 605
BseAI TCCGGA 1 cut(s) 385
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 5 cut(s) 117, 162, 201, 376, 489
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMII CTCAG 4 cut(s) 169, 417, 466, 617
BseNI ACTGG 3 cut(s) 21, 103, 605
BseXI GCAGC 4 cut(s) 18, 135, 170, 297
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 3 cut(s) 12, 177, 375
BsiSI CCGG 3 cut(s) 246, 386, 701
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 776
BsmBI CGTCTC 1 cut(s) 776
BsnI GGCC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 3 cut(s) 12, 177, 375
Bsp13I TCCGGA 1 cut(s) 385
Bsp143I GATC 1 cut(s) 158
Bsp19I CCATGG 2 cut(s) 117, 201
BspACI CCGC 3 cut(s) 93, 181, 190
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 4 cut(s) 168, 418, 465, 618
BspEI TCCGGA 1 cut(s) 385
BspLI GGNNCC 1 cut(s) 756
BspPI GGATC 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 309
BsrI ACTGG 3 cut(s) 21, 103, 605
BssECI CCNNGG 5 cut(s) 117, 162, 201, 376, 489
BssMI GATC 1 cut(s) 158
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 4 cut(s) 117, 201, 376, 489
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 5 cut(s) 295, 472, 562, 586, 673
BstACI GRCGYC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 309
BstDEI CTNAG 5 cut(s) 155, 426, 452, 626, 759
BstDSI CCRYGG 2 cut(s) 117, 201
BstH2I RGCGCY 2 cut(s) 140, 149
BstHHI GCGC 3 cut(s) 139, 148, 245
BstKTI GATC 1 cut(s) 161
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 1 cut(s) 158
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 4 cut(s) 143, 145, 189, 431
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 2 cut(s) 544, 558
BstV1I GCAGC 4 cut(s) 18, 135, 170, 297
BstV2I GAAGAC 1 cut(s) 681
BstX2I RGATCY 1 cut(s) 158
BstXI CCANNNNNNTGG 1 cut(s) 133
BstYI RGATCY 1 cut(s) 158
BsuRI GGCC 1 cut(s) 116
BtgI CCRYGG 2 cut(s) 117, 201
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 4 cut(s) 96, 460, 528, 567
CfoI GCGC 3 cut(s) 139, 148, 245
Cfr13I GGNCC 1 cut(s) 61
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 631, 715
CviAII CATG 7 cut(s) 118, 202, 208, 223, 304, 401, 634
CviQI GTAC 2 cut(s) 631, 715
DdeI CTNAG 5 cut(s) 155, 426, 452, 626, 759
DpnI GATC 1 cut(s) 160
DpnII GATC 1 cut(s) 158
DrdI GACNNNNNNGTC 1 cut(s) 661
DseDI GACNNNNNNGTC 1 cut(s) 661
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 4 cut(s) 117, 201, 376, 489
Eco24I GRGCYC 1 cut(s) 12
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 3 cut(s) 56, 405, 594
Eco72I CACGTG 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 61
EcoRI GAATTC 2 cut(s) 381, 637
EcoRII CCWGG 1 cut(s) 111
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 4 cut(s) 117, 201, 376, 489
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 4 cut(s) 117, 201, 376, 489
Esp3I CGTCTC 1 cut(s) 776
FaeI CATG 7 cut(s) 121, 205, 211, 226, 307, 404, 637
FaiI YATR 9 cut(s) 119, 203, 209, 224, 305, 402, 442, 596, 635
FatI CATG 7 cut(s) 117, 201, 207, 222, 303, 400, 633
Fnu4HI GCNGC 4 cut(s) 32, 149, 184, 286
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 4 cut(s) 32, 149, 184, 286
FspBI CTAG 1 cut(s) 435
GlaI GCGC 3 cut(s) 138, 147, 244
GluI GCNGC 4 cut(s) 32, 149, 184, 286
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 2 cut(s) 140, 149
HaeIII GGCC 1 cut(s) 116
HapII CCGG 3 cut(s) 246, 386, 701
HgaI GACGC 1 cut(s) 83
HhaI GCGC 3 cut(s) 139, 148, 245
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 7 cut(s) 121, 205, 211, 226, 307, 404, 637
Hin6I GCGC 3 cut(s) 137, 146, 243
HinP1I GCGC 3 cut(s) 137, 146, 243
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 2 cut(s) 50, 122
HpaII CCGG 3 cut(s) 246, 386, 701
HphI GGTGA 3 cut(s) 208, 289, 476
Hpy166II GTNNAC 2 cut(s) 70, 565
Hpy188I TCNGA 6 cut(s) 14, 158, 163, 337, 627, 694
Hpy188III TCNNGA 2 cut(s) 47, 386
Hpy8I GTNNAC 2 cut(s) 70, 565
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 5 cut(s) 34, 310, 429, 488, 652
HpyCH4III ACNGT 5 cut(s) 295, 472, 562, 586, 673
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 1 cut(s) 725
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 145, 189, 431
HpyF3I CTNAG 5 cut(s) 155, 426, 452, 626, 759
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 7 cut(s) 121, 205, 211, 226, 307, 404, 637
HspAI GCGC 3 cut(s) 137, 146, 243
Kpn2I TCCGGA 1 cut(s) 385
Kzo9I GATC 1 cut(s) 158
LmnI GCTCC 4 cut(s) 7, 15, 95, 760
Lsp1109I GCAGC 4 cut(s) 18, 135, 170, 297
LweI GCATC 2 cut(s) 222, 405
MaeI CTAG 1 cut(s) 435
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 295, 484, 673
MalI GATC 1 cut(s) 160
MboI GATC 1 cut(s) 158
MboII GAAGA 6 cut(s) 49, 587, 681, 779, 782, 788
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 3 cut(s) 12, 177, 375
MluCI AATT 3 cut(s) 330, 381, 637
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 8 cut(s) 74, 119, 157, 224, 382, 461, 509, 621
MroI TCCGGA 1 cut(s) 385
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 4 cut(s) 206, 221, 302, 560
MspA1I CMGCKG 2 cut(s) 183, 425
MspI CCGG 3 cut(s) 246, 386, 701
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
MwoI GCNNNNNNNGC 4 cut(s) 143, 145, 189, 431
NcoI CCATGG 2 cut(s) 117, 201
NdeII GATC 1 cut(s) 158
NlaIII CATG 7 cut(s) 121, 205, 211, 226, 307, 404, 637
NlaIV GGNNCC 1 cut(s) 756
NmuCI GTSAC 2 cut(s) 295, 484
NspI RCATGY 1 cut(s) 226
NspV TTCGAA 1 cut(s) 309
OliI CACNNNNGTG 1 cut(s) 560
PciI ACATGT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 285
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 1 cut(s) 122
PflFI GACNNNGTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 4 cut(s) 33, 150, 185, 287
PleI GAGTC 1 cut(s) 58
PmaCI CACGTG 1 cut(s) 133
PmlI CACGTG 1 cut(s) 133
PpsI GAGTC 1 cut(s) 58
Ppu21I YACGTR 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 61
PscI ACATGT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 111
PspCI CACGTG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 756
PspPI GGNCC 1 cut(s) 61
PspPPI RGGWCCY 1 cut(s) 61
PsuI RGATCY 1 cut(s) 158
PsyI GACNNNGTC 1 cut(s) 65
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 2 cut(s) 632, 716
RsaNI GTAC 2 cut(s) 631, 715
RseI CAYNNNNRTG 4 cut(s) 206, 221, 302, 560
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 4 cut(s) 32, 149, 184, 286
Sau3AI GATC 1 cut(s) 158
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 3 cut(s) 12, 177, 375
SfaNI GCATC 2 cut(s) 222, 405
SfcI CTRYAG 2 cut(s) 544, 558
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 4 cut(s) 206, 221, 302, 560
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 3 cut(s) 330, 381, 637
SsiI CCGC 3 cut(s) 93, 181, 190
SspMI CTAG 1 cut(s) 435
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 4 cut(s) 117, 201, 376, 489
TaaI ACNGT 5 cut(s) 295, 472, 562, 586, 673
TaiI ACGT 1 cut(s) 135
TaqI TCGA 4 cut(s) 84, 171, 309, 665
TasI AATT 3 cut(s) 330, 381, 637
TatI WGTACW 1 cut(s) 714
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 4 cut(s) 103, 460, 535, 567
TseFI GTSAC 2 cut(s) 295, 484
TseI GCWGC 4 cut(s) 31, 148, 183, 285
Tsp45I GTSAC 2 cut(s) 295, 484
TspDTI ATGAA 7 cut(s) 224, 292, 362, 600, 611, 650, 789
TspRI CASTG 4 cut(s) 103, 460, 535, 567
Tth111I GACNNNGTC 1 cut(s) 65
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 3 cut(s) 330, 381, 637
XceI RCATGY 1 cut(s) 226
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.