Rmu_sc0000435.1_g000036

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000435.1
OG0011395
Physical Location & Seq
Forward (+)
165464 .. 167138
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000435.1_g000036.1.cds

Sequence Viewer

Length: 801 bp
atgttggagctccgattggtccagggctccctcctcaagaaggttctcgagtccatcaaggacctcgtcaccgacgccaacttcgactgctccgccaccgggttctccctccaggccatggactccagccacgtcgccctcgtcgccctccgcctcagatctgaagggttcgagcactaccgctgcgatcggagcatctccatggggatgaacctcggcaacatgtccaagatgctcaagtgcgccggcaacgatgacatcatcaccatcaaggctgacgatgggagcgatactgtcaccttcatgtttgagagccccacgcaagataaaatttctgattttgagatgaagctgatggacattgatagtgagcaccttggaattccggaggctgaataccatgcaattgttaggatgccttctgctgaatttgccaggatttgcaaagacctcagcggcatcggtgatactgttgtgatatctgtgacaaaggaaggtgtgaagttctctacaagaggggatatcggcactgctaatatcgtctgtaggcagaacactactgttgacaagcctgaagaagcaacaatcatagagatgaatgagccagtttcattgacatttgctctgaggtacatgaactccttcacaaaagcatcccccctgtcaaacacagtcactatcagcctctcttcggaactaccagttgtggttgagtacaagattgctgagatgggctacataaggttctacttggctccgaaggtagaagaggatgaagatgagacaaagcctcaagtttga

Protein Analysis

266

Amino Acids

29.46

Weight (kDa)

4.72

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07370 AT2G29570
fragaria_vesca FvH4_3g37710 FvH4_6g24870
malus_domestica MD03G1078300.v1.1 MD11G1083000.v1.1 MD13G1227300.v1.1
prunus_persica Prupe.6G063300_v2.0.a1
pyrus_communis pycom03g06260 pycom11g07110
rosa_chinensis RchiOBHm_Chr5g0067981
rosa_laevigata RLG00000035949
rosa_multiflora Rmu_sc0000435.1_g000036
rosa_roxburghii Rroxscaffold_1G00012620 Rroxscaffold_1G00013330
rosa_rugosa Rorug05G0387000
rosa_samantha Rh5BG463400 Rh5CG485300 Rh5DG477400
rosa_wichuraiana Rw5G041640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 118
AccIII TCCGGA 1 cut(s) 385
AciI CCGC 4 cut(s) 93, 151, 181, 456
AcsI RAATTY 3 cut(s) 330, 381, 428
AcuI CTGAAG 2 cut(s) 183, 594
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 632, 716
AfiI CCNNNNNNNGG 4 cut(s) 40, 99, 118, 691
AflIII ACRYGT 1 cut(s) 222
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 3 cut(s) 21, 111, 434
AluBI AGCT 2 cut(s) 10, 352
AluI AGCT 2 cut(s) 10, 352
Alw21I GWGCWC 3 cut(s) 12, 177, 375
Alw26I GTCTC 1 cut(s) 776
Ama87I CYCGRG 1 cut(s) 47
Aor13HI TCCGGA 1 cut(s) 385
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 1 cut(s) 183
ApoI RAATTY 3 cut(s) 330, 381, 428
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp700I GAANNNNTTC 1 cut(s) 641
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 19, 61
AsuC2I CCSGG 1 cut(s) 100
AsuHPI GGTGA 4 cut(s) 61, 256, 289, 476
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 2 cut(s) 19, 61
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BanII GRGCYC 3 cut(s) 12, 29, 317
Bbv12I GWGCWC 3 cut(s) 12, 177, 375
BbvCI CCTCAGC 1 cut(s) 452
BbvI GCAGC 1 cut(s) 170
BccI CCATC 5 cut(s) 62, 275, 275, 349, 724
BciT130I CCWGG 3 cut(s) 23, 113, 436
BcnI CCSGG 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 776
BfmI CTRYAG 1 cut(s) 544
BglII AGATCT 1 cut(s) 158
BisI GCNGC 2 cut(s) 184, 457
BlsI GCNGC 2 cut(s) 185, 458
Bme1390I CCNGG 4 cut(s) 23, 100, 113, 436
Bme18I GGWCC 2 cut(s) 19, 61
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 2 cut(s) 19, 61
BmiI GGNNCC 2 cut(s) 28, 756
BmrFI CCNGG 4 cut(s) 23, 100, 113, 436
BmsI GCATC 5 cut(s) 204, 222, 405, 468, 662
BpmI CTGGAG 2 cut(s) 95, 109
Bpu10I CCTNAGC 1 cut(s) 452
BpuEI CTTGAG 3 cut(s) 20, 221, 777
BpuMI CCSGG 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 5 cut(s) 22, 117, 201, 214, 376
BsaWI WCCGGW 1 cut(s) 385
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 99, 118, 691
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 2 cut(s) 605, 701
BseAI TCCGGA 1 cut(s) 385
BseBI CCWGG 3 cut(s) 23, 113, 436
BseDI CCNNGG 5 cut(s) 22, 117, 201, 214, 376
BseGI GGATG 4 cut(s) 213, 420, 653, 778
BseLI CCNNNNNNNGG 4 cut(s) 40, 99, 118, 691
BseMII CTCAG 4 cut(s) 169, 466, 617, 717
BseNI ACTGG 2 cut(s) 605, 701
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 170
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 3 cut(s) 12, 177, 375
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 3 cut(s) 99, 246, 386
BslI CCNNNNNNNGG 4 cut(s) 40, 99, 118, 691
BsmAI GTCTC 1 cut(s) 776
BsnI GGCC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 5 cut(s) 12, 29, 177, 317, 375
Bsp13I TCCGGA 1 cut(s) 385
Bsp143I GATC 2 cut(s) 158, 187
Bsp19I CCATGG 2 cut(s) 117, 201
BspACI CCGC 4 cut(s) 93, 151, 181, 456
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 4 cut(s) 168, 465, 618, 718
BspEI TCCGGA 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 28, 756
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 2 cut(s) 605, 701
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 5 cut(s) 22, 117, 201, 214, 376
BssMI GATC 2 cut(s) 158, 187
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 3 cut(s) 117, 201, 376
Bst2UI CCWGG 3 cut(s) 23, 113, 436
Bst4CI ACNGT 4 cut(s) 295, 472, 562, 673
Bst6I CTCTTC 2 cut(s) 694, 762
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 247
BstDEI CTNAG 4 cut(s) 155, 452, 626, 726
BstDSI CCRYGG 2 cut(s) 117, 201
BstENI CCTNNNNNAGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 213, 420, 653, 778
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 2 cut(s) 161, 190
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 2 cut(s) 158, 187
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 3 cut(s) 143, 192, 431
BstNI CCWGG 3 cut(s) 23, 113, 436
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 4 cut(s) 21, 98, 111, 434
BstSFI CTRYAG 1 cut(s) 544
BstV1I GCAGC 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
BsuRI GGCC 1 cut(s) 116
BtgI CCRYGG 2 cut(s) 117, 201
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 4 cut(s) 213, 420, 653, 778
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 247
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 19, 61
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 631, 715
CviAII CATG 6 cut(s) 118, 202, 223, 304, 401, 634
CviQI GTAC 2 cut(s) 631, 715
DdeI CTNAG 4 cut(s) 155, 452, 626, 726
DpnI GATC 2 cut(s) 160, 189
DpnII GATC 2 cut(s) 158, 187
Eam1104I CTCTTC 2 cut(s) 694, 762
EarI CTCTTC 2 cut(s) 694, 762
EciI GGCGGA 2 cut(s) 82, 140
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 3 cut(s) 117, 201, 376
Eco24I GRGCYC 3 cut(s) 12, 29, 317
Eco32I GATATC 2 cut(s) 480, 523
Eco47I GGWCC 2 cut(s) 19, 61
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 2 cut(s) 183, 594
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 381
EcoRII CCWGG 3 cut(s) 21, 111, 434
EcoRV GATATC 2 cut(s) 480, 523
EcoT14I CCWWGG 3 cut(s) 117, 201, 376
EcoT38I GRGCYC 3 cut(s) 12, 29, 317
ErhI CCWWGG 3 cut(s) 117, 201, 376
FaeI CATG 6 cut(s) 121, 205, 226, 307, 404, 637
FaiI YATR 8 cut(s) 119, 203, 224, 305, 402, 590, 635, 740
FatI CATG 6 cut(s) 117, 201, 222, 303, 400, 633
Fnu4HI GCNGC 2 cut(s) 184, 457
FokI GGATG 4 cut(s) 220, 427, 640, 785
FriOI GRGCYC 3 cut(s) 12, 29, 317
Fsp4HI GCNGC 2 cut(s) 184, 457
GlaI GCGC 1 cut(s) 244
GluI GCNGC 2 cut(s) 184, 457
GsuI CTGGAG 2 cut(s) 95, 109
HaeIII GGCC 1 cut(s) 116
HapII CCGG 3 cut(s) 99, 246, 386
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 6 cut(s) 121, 205, 226, 307, 404, 637
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 2 cut(s) 50, 122
HpaII CCGG 3 cut(s) 99, 246, 386
HphI GGTGA 4 cut(s) 61, 256, 289, 476
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 8 cut(s) 14, 158, 163, 192, 337, 627, 694, 759
Hpy188III TCNNGA 3 cut(s) 37, 47, 386
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 3 cut(s) 77, 137, 146
HpyAV CCTTC 7 cut(s) 34, 158, 310, 429, 488, 652, 754
HpyCH4III ACNGT 4 cut(s) 295, 472, 562, 673
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 2 cut(s) 404, 444
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 192, 431
HpyF3I CTNAG 4 cut(s) 155, 452, 626, 726
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 6 cut(s) 121, 205, 226, 307, 404, 637
HspAI GCGC 1 cut(s) 243
Kpn2I TCCGGA 1 cut(s) 385
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 158, 187
LmnI GCTCC 7 cut(s) 7, 15, 32, 95, 192, 285, 760
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 5 cut(s) 204, 222, 405, 468, 662
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 4 cut(s) 67, 295, 484, 673
MalI GATC 2 cut(s) 160, 189
MboI GATC 2 cut(s) 158, 187
MboII GAAGA 4 cut(s) 587, 681, 779, 788
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 5 cut(s) 12, 29, 177, 317, 375
MluCI AATT 4 cut(s) 330, 381, 405, 428
MlyI GAGTC 2 cut(s) 59, 116
MroI TCCGGA 1 cut(s) 385
MroNI GCCGGC 1 cut(s) 245
MroXI GAANNNNTTC 1 cut(s) 641
MslI CAYNNNNRTG 4 cut(s) 200, 206, 302, 593
MspA1I CMGCKG 2 cut(s) 183, 456
MspI CCGG 3 cut(s) 99, 246, 386
MspR9I CCNGG 4 cut(s) 23, 100, 113, 436
MunI CAATTG 1 cut(s) 405
MvaI CCWGG 3 cut(s) 23, 113, 436
MwoI GCNNNNNNNGC 3 cut(s) 143, 192, 431
NaeI GCCGGC 1 cut(s) 247
NciI CCSGG 1 cut(s) 100
NcoI CCATGG 2 cut(s) 117, 201
NdeII GATC 2 cut(s) 158, 187
NgoMIV GCCGGC 1 cut(s) 245
NlaIII CATG 6 cut(s) 121, 205, 226, 307, 404, 637
NlaIV GGNNCC 2 cut(s) 28, 756
NmeAIII GCCGAG 1 cut(s) 195
NmuCI GTSAC 4 cut(s) 67, 295, 484, 673
NspI RCATGY 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 47
PciI ACATGT 1 cut(s) 222
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 138, 285
PdiI GCCGGC 1 cut(s) 247
PdmI GAANNNNTTC 1 cut(s) 641
PflFI GACNNNGTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 2 cut(s) 185, 458
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 2 cut(s) 58, 116
PpsI GAGTC 2 cut(s) 58, 116
PpuMI RGGWCCY 1 cut(s) 61
PscI ACATGT 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 3 cut(s) 21, 111, 434
PspGI CCWGG 3 cut(s) 21, 111, 434
PspN4I GGNNCC 2 cut(s) 28, 756
PspPI GGNCC 2 cut(s) 19, 61
PspPPI RGGWCCY 1 cut(s) 61
PsuI RGATCY 1 cut(s) 158
PsyI GACNNNGTC 1 cut(s) 65
PvuI CGATCG 1 cut(s) 190
RsaI GTAC 2 cut(s) 632, 716
RsaNI GTAC 2 cut(s) 631, 715
RseI CAYNNNNRTG 4 cut(s) 200, 206, 302, 593
SacI GAGCTC 1 cut(s) 12
SatI GCNGC 2 cut(s) 184, 457
Sau3AI GATC 2 cut(s) 158, 187
Sau96I GGNCC 2 cut(s) 19, 61
SchI GAGTC 2 cut(s) 59, 116
ScrFI CCNGG 4 cut(s) 23, 100, 113, 436
SduI GDGCHC 5 cut(s) 12, 29, 177, 317, 375
SfaNI GCATC 5 cut(s) 204, 222, 405, 468, 662
SfcI CTRYAG 1 cut(s) 544
Sfr274I CTCGAG 1 cut(s) 47
SinI GGWCC 2 cut(s) 19, 61
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 4 cut(s) 200, 206, 302, 593
SmlI CTYRAG 4 cut(s) 35, 47, 236, 792
SmoI CTYRAG 4 cut(s) 35, 47, 236, 792
Sse9I AATT 4 cut(s) 330, 381, 405, 428
SsiI CCGC 4 cut(s) 93, 151, 181, 456
SstI GAGCTC 1 cut(s) 12
StyD4I CCNGG 4 cut(s) 21, 98, 111, 434
StyI CCWWGG 3 cut(s) 117, 201, 376
TaaI ACNGT 4 cut(s) 295, 472, 562, 673
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 48, 84, 171
TasI AATT 4 cut(s) 330, 381, 405, 428
TatI WGTACW 1 cut(s) 714
TauI GCSGC 1 cut(s) 459
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 4 cut(s) 67, 295, 484, 673
TseI GCWGC 1 cut(s) 183
Tsp45I GTSAC 4 cut(s) 67, 295, 484, 673
TspDTI ATGAA 7 cut(s) 224, 292, 362, 600, 611, 650, 789
TspRI CASTG 1 cut(s) 535
Tth111I GACNNNGTC 1 cut(s) 65
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 19, 61
XagI CCTNNNNNAGG 1 cut(s) 38
XapI RAATTY 3 cut(s) 330, 381, 428
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.