Prupe.6G099600_v2.0.a1

DNA polymerase epsilon subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0009988
Physical Location & Seq
Reverse (-)
6922369 .. 6925009
2641 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G099600.1

Sequence Viewer

Length: 666 bp
ATGGCGACGCCCGGAAAGGCCACAACACCAAAGAAGAGCAAAGAAACAAAGAAGCCGAGCAGCAAAGCCATTACACCGAAGAAAAACCAAGAAACAAAGAAGCCAAGCATCAAGAAAACCACAGAAACAAAGAAGCCCGGCACCAAGAAAACGATCAGCGACAAGAACCAGACCAGCAATGGCAATGTGAGCGACTCCGACGTAGAGGAGATTCCATCGTCGAGCTCCGAGTCCCTGAAAGACACAAAGCAAAATGGGGGTAAAATTACGAAAACGCCCACCCCGAAATCGAAGCAGAGCAACAAGAAACGAAAGCAGAAGCAAGAAGAAGAAGAAGAGGTCGAAGAGAAAGAGGAGATGAAGTCGTATACGTTTCCGATGGAGCGCGTTAAGAGGATTATCAGAAGCGAAGATTCGGAGATGCGCATTAGCCACGACGCCGTTTTCCTCGTCAACAAAGCCACTGAGAAGTTCCTTCAGAAGTTTTGTGAAGATGCACATGCTTGTTGTGTTAAGGATAGGAAAAAGTCTCTTGCTTACAAGCACCTATCCACAGTTGTCTCTAAACGAAAGAGATATGACTTTCTTTCAGATTATGTTCCTGAGAAAGTAAAAGCGGAGGATGCATTGGCAAAGAGAAAATTAGCCGAAGCAAAAGCGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

222

Amino Acids

25.05

Weight (kDa)

9.67

Isoelectric Point (pI)

53.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 140
AccI GTMKAC 1 cut(s) 368
AccII CGCG 1 cut(s) 387
AciI CCGC 2 cut(s) 617, 659
AcuI CTGAAG 1 cut(s) 461
AcyI GRCGYC 2 cut(s) 8, 438
AluBI AGCT 1 cut(s) 225
AluI AGCT 1 cut(s) 225
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 2 cut(s) 534, 565
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 60
AspLEI GCGC 2 cut(s) 387, 426
AsuC2I CCSGG 2 cut(s) 12, 138
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 72
BccI CCATC 2 cut(s) 223, 373
BceAI ACGGC 1 cut(s) 425
BcnI CCSGG 2 cut(s) 12, 138
BcoDI GTCTC 2 cut(s) 534, 565
BfaI CTAG 1 cut(s) 664
BisI GCNGC 1 cut(s) 61
BlsI GCNGC 1 cut(s) 62
Bme1390I CCNGG 2 cut(s) 12, 138
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 12, 138
BmsI GCATC 4 cut(s) 117, 411, 484, 613
BpuMI CCSGG 2 cut(s) 12, 138
BsaHI GRCGYC 2 cut(s) 8, 438
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bse3DI GCAATG 2 cut(s) 184, 190
BseGI GGATG 1 cut(s) 628
BseMI GCAATG 2 cut(s) 184, 190
BseMII CTCAG 2 cut(s) 456, 594
BseRI GAGGAG 2 cut(s) 221, 368
BseXI GCAGC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 2 cut(s) 12, 138
BslFI GGGAC 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 534, 565
BsmFI GGGAC 1 cut(s) 217
BsnI GGCC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 2 cut(s) 617, 659
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 457, 595
BspFNI CGCG 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 142
BspQI GCTCTTC 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 140
BsrDI GCAATG 2 cut(s) 184, 190
BssMI GATC 1 cut(s) 153
BssNAI GTATAC 1 cut(s) 369
BssNI GRCGYC 2 cut(s) 8, 438
Bst1107I GTATAC 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 556
Bst6I CTCTTC 3 cut(s) 29, 330, 339
BstACI GRCGYC 2 cut(s) 8, 438
BstDEI CTNAG 2 cut(s) 465, 603
BstF5I GGATG 1 cut(s) 628
BstFNI CGCG 1 cut(s) 387
BstHHI GCGC 2 cut(s) 387, 426
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 2 cut(s) 534, 565
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 2 cut(s) 189, 623
BstNSI RCATGY 1 cut(s) 503
BstSCI CCNGG 2 cut(s) 10, 136
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 72
BstZ17I GTATAC 1 cut(s) 369
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 1 cut(s) 628
BtsIMutI CAGTG 1 cut(s) 462
CfoI GCGC 2 cut(s) 387, 426
CseI GACGC 2 cut(s) 16, 446
CviAII CATG 1 cut(s) 500
CviJI RGCY 9 cut(s) 20, 55, 68, 103, 136, 225, 432, 461, 647
CviKI_1 RGCY 9 cut(s) 20, 55, 68, 103, 136, 225, 432, 461, 647
DdeI CTNAG 2 cut(s) 465, 603
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
Eam1104I CTCTTC 3 cut(s) 29, 330, 339
EarI CTCTTC 3 cut(s) 29, 330, 339
Ecl136II GAGCTC 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 227
Eco53kI GAGCTC 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 225
EcoT22I ATGCAT 1 cut(s) 628
EcoT38I GRGCYC 1 cut(s) 227
FaeI CATG 1 cut(s) 503
FaiI YATR 4 cut(s) 369, 501, 579, 597
FaqI GGGAC 1 cut(s) 217
FatI CATG 1 cut(s) 499
FblI GTMKAC 1 cut(s) 368
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 1 cut(s) 635
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 1 cut(s) 61
FspAI RTGCGCAY 1 cut(s) 425
FspBI CTAG 1 cut(s) 664
FspI TGCGCA 1 cut(s) 425
GlaI GCGC 2 cut(s) 386, 425
GluI GCNGC 1 cut(s) 61
HaeIII GGCC 1 cut(s) 20
HapII CCGG 2 cut(s) 12, 138
HgaI GACGC 2 cut(s) 16, 446
HhaI GCGC 2 cut(s) 387, 426
Hin1I GRCGYC 2 cut(s) 8, 438
Hin1II CATG 1 cut(s) 503
Hin6I GCGC 2 cut(s) 385, 424
HinP1I GCGC 2 cut(s) 385, 424
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 4 cut(s) 194, 211, 230, 413
HpaII CCGG 2 cut(s) 12, 138
Hpy166II GTNNAC 2 cut(s) 369, 454
Hpy188I TCNGA 7 cut(s) 199, 229, 378, 404, 418, 480, 592
Hpy188III TCNNGA 2 cut(s) 112, 602
Hpy8I GTNNAC 2 cut(s) 369, 454
Hpy99I CGWCG 4 cut(s) 10, 203, 223, 440
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 1 cut(s) 556
HpyCH4IV ACGT 2 cut(s) 201, 371
HpyCH4V TGCA 2 cut(s) 497, 626
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 623
HpyF3I CTNAG 2 cut(s) 465, 603
HpySE526I ACGT 2 cut(s) 201, 371
Hsp92I GRCGYC 2 cut(s) 8, 438
Hsp92II CATG 1 cut(s) 503
HspAI GCGC 2 cut(s) 385, 424
Kzo9I GATC 1 cut(s) 153
LguI GCTCTTC 1 cut(s) 29
LmnI GCTCC 2 cut(s) 230, 382
LpnPI CCDG 6 cut(s) 25, 151, 182, 187, 248, 615
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 4 cut(s) 117, 411, 484, 613
MaeI CTAG 1 cut(s) 664
MaeII ACGT 2 cut(s) 201, 371
MalI GATC 1 cut(s) 155
MboI GATC 1 cut(s) 153
MboII GAAGA 9 cut(s) 46, 91, 338, 341, 344, 347, 356, 422, 503
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 2 cut(s) 264, 641
MlyI GAGTC 2 cut(s) 188, 239
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 6 cut(s) 199, 331, 346, 387, 458, 613
Mph1103I ATGCAT 1 cut(s) 628
MseI TTAA 2 cut(s) 390, 513
MspI CCGG 2 cut(s) 12, 138
MspR9I CCNGG 2 cut(s) 12, 138
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 2 cut(s) 189, 623
NciI CCSGG 2 cut(s) 12, 138
NdeII GATC 1 cut(s) 153
NlaIII CATG 1 cut(s) 503
NlaIV GGNNCC 1 cut(s) 142
NmeAIII GCCGAG 1 cut(s) 81
NsbI TGCGCA 1 cut(s) 425
NsiI ATGCAT 1 cut(s) 628
NspI RCATGY 1 cut(s) 503
PciSI GCTCTTC 1 cut(s) 29
PcsI WCGNNNNNNNCGW 1 cut(s) 281
PfeI GAWTC 2 cut(s) 211, 413
PkrI GCNGC 1 cut(s) 62
PleI GAGTC 2 cut(s) 188, 238
PpsI GAGTC 2 cut(s) 188, 238
Psp124BI GAGCTC 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 142
SacI GAGCTC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 29
SaqAI TTAA 2 cut(s) 390, 513
SatI GCNGC 1 cut(s) 61
Sau3AI GATC 1 cut(s) 153
SchI GAGTC 2 cut(s) 188, 239
ScrFI CCNGG 2 cut(s) 12, 138
SduI GDGCHC 1 cut(s) 227
SetI ASST 5 cut(s) 204, 227, 342, 374, 549
SfaNI GCATC 4 cut(s) 117, 411, 484, 613
Sse9I AATT 2 cut(s) 264, 641
SsiI CCGC 2 cut(s) 617, 659
SspMI CTAG 1 cut(s) 664
SstI GAGCTC 1 cut(s) 227
StyD4I CCNGG 2 cut(s) 10, 136
TaaI ACNGT 1 cut(s) 556
TaiI ACGT 2 cut(s) 204, 374
TaqI TCGA 3 cut(s) 221, 290, 342
TasI AATT 2 cut(s) 264, 641
TfiI GAWTC 2 cut(s) 211, 413
Tru1I TTAA 2 cut(s) 390, 513
Tru9I TTAA 2 cut(s) 390, 513
TscAI CASTG 1 cut(s) 469
TseI GCWGC 1 cut(s) 60
TspDTI ATGAA 1 cut(s) 374
TspRI CASTG 1 cut(s) 469
XceI RCATGY 1 cut(s) 503
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmiI GTMKAC 1 cut(s) 368
XspI CTAG 1 cut(s) 664
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.