Rorug05G0324300

DNA polymerase epsilon subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009988
Physical Location & Seq
Forward (+)
38915506 .. 38918060
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0324300.1

Sequence Viewer

Length: 750 bp
ATGTCATGGAATTTTCTGGCTTCAGCCATCCGAGACAAGTTCGTCTTAGCAGGAGTACAACATTTCTTTGATTCTGTTGGAAAGTCAATCTCCAATGAGAGCTTCAAGAGCCGTAGACAGCGACAGAGAGAGCTAAGGAAACGACCTCGCAGCTTTCAAGTTTTGAGCTGCTTAACCAAAACAGAGCTTGCAGCGATGGAGACAGAAACGGCGGCGATTCTAAGCGACCTAGGTGCATTGACTGTTATGATGAGAAATACAAGACTATACAAATTTGGGTGCAACTGTGCAAAGAAGATGGTTGTGATAAATTTAGGACCTATTGAATGTATACCCTTTCAGAGGGAAGGTGCCTTTGGATGTGCAACACTACTTGATAAAAGCCTACCAATTGAAGAGGGTGATGCTCGCTATATGCCTTCAGAGATCCTGAATGAGAAACATGACGATCTTGACAAGGCTGATATCTTCTCATTGGGAGTTGCTATGTATGAGCCTATTAGAGGTTCACCGTTACCGGAAGAAGGGCCTCAAACCTTAAATCTTAAGAAAGGAAAATTGCCACTGCTTCCTGGTCACTCCTTGCAACTTCAGAACTTGCTCAAGGCCATGTTGGATCCAAATCCAGTCTGCAGACCATCAGCTAAAGAGTTGGTTGAAAATCTAATGTTCCACAGGGTGCTAAAAAATGTATGGGCCTGGGACAACCACGATTGCGAATTGAAGAGATTGGGGTATATTGTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

249

Amino Acids

28.16

Weight (kDa)

8.8

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 119 - 221 1.8e-08 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 350
AccI GTMKAC 2 cut(s) 115, 331
AciI CCGC 1 cut(s) 212
AclWI GGATC 3 cut(s) 421, 611, 624
AcsI RAATTY 3 cut(s) 10, 272, 310
AcuI CTGAAG 3 cut(s) 6, 405, 575
AdeI CACNNNGTG 1 cut(s) 679
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 3 cut(s) 342, 503, 524
AflII CTTAAG 1 cut(s) 545
AgsI TTSAA 6 cut(s) 106, 158, 326, 395, 659, 724
AjnI CCWGG 2 cut(s) 571, 698
AjuI GAANNNNNNNTTGG 4 cut(s) 86, 118, 339, 371
AluBI AGCT 6 cut(s) 102, 133, 153, 168, 187, 644
AluI AGCT 6 cut(s) 102, 133, 153, 168, 187, 644
Alw26I GTCTC 2 cut(s) 27, 194
AlwI GGATC 3 cut(s) 421, 611, 624
AoxI GGCC 3 cut(s) 527, 606, 696
ApeKI GCWGC 3 cut(s) 150, 168, 191
ApoI RAATTY 3 cut(s) 10, 272, 310
AspA2I CCTAGG 1 cut(s) 229
AspS9I GGNCC 3 cut(s) 317, 527, 696
AsuHPI GGTGA 2 cut(s) 413, 501
AvaII GGWCC 1 cut(s) 317
AvrII CCTAGG 1 cut(s) 229
BamHI GGATCC 1 cut(s) 616
BanI GGYRCC 1 cut(s) 350
BbvI GCAGC 3 cut(s) 155, 162, 203
BccI CCATC 4 cut(s) 35, 190, 292, 646
BceAI ACGGC 2 cut(s) 96, 225
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 573, 700
BcoDI GTCTC 2 cut(s) 27, 194
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 631
BfrI CTTAAG 1 cut(s) 545
BisI GCNGC 4 cut(s) 151, 169, 192, 213
BlnI CCTAGG 1 cut(s) 229
BlsI GCNGC 4 cut(s) 152, 170, 193, 214
Bme1390I CCNGG 2 cut(s) 573, 700
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 317, 527, 696
BmiI GGNNCC 2 cut(s) 352, 618
BmrFI CCNGG 2 cut(s) 573, 700
BmsI GCATC 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 587
BsaBI GATNNNNATC 1 cut(s) 621
BsaJI CCNNGG 2 cut(s) 229, 699
BsaWI WCCGGW 1 cut(s) 517
Bsc4I CCNNNNNNNGG 3 cut(s) 342, 503, 524
Bse1I ACTGG 1 cut(s) 626
Bse8I GATNNNNATC 1 cut(s) 621
BseBI CCWGG 2 cut(s) 573, 700
BseDI CCNNGG 2 cut(s) 229, 699
BseGI GGATG 2 cut(s) 27, 365
BseJI GATNNNNATC 1 cut(s) 621
BseLI CCNNNNNNNGG 3 cut(s) 342, 503, 524
BseNI ACTGG 1 cut(s) 626
BseXI GCAGC 3 cut(s) 155, 162, 203
BshFI GGCC 3 cut(s) 529, 608, 698
BshNI GGYRCC 1 cut(s) 350
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 1 cut(s) 716
BslI CCNNNNNNNGG 3 cut(s) 342, 503, 524
BsmAI GTCTC 2 cut(s) 27, 194
BsmFI GGGAC 1 cut(s) 716
BsnI GGCC 3 cut(s) 529, 608, 698
Bsp143I GATC 3 cut(s) 426, 448, 616
BspACI CCGC 1 cut(s) 212
BspANI GGCC 3 cut(s) 529, 608, 698
BspLI GGNNCC 2 cut(s) 352, 618
BspMAI CTGCAG 1 cut(s) 635
BspPI GGATC 3 cut(s) 421, 611, 624
BspT107I GGYRCC 1 cut(s) 350
BspTI CTTAAG 1 cut(s) 545
BsrI ACTGG 1 cut(s) 626
BssECI CCNNGG 2 cut(s) 229, 699
BssMI GATC 3 cut(s) 426, 448, 616
BssNAI GTATAC 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 229
Bst1107I GTATAC 1 cut(s) 332
Bst2UI CCWGG 2 cut(s) 573, 700
Bst4CI ACNGT 3 cut(s) 244, 287, 513
Bst6I CTCTTC 2 cut(s) 390, 719
BstAFI CTTAAG 1 cut(s) 545
BstC8I GCNNGC 2 cut(s) 189, 409
BstDEI CTNAG 3 cut(s) 46, 134, 221
BstENI CCTNNNNNAGG 2 cut(s) 340, 501
BstF5I GGATG 2 cut(s) 27, 365
BstKTI GATC 3 cut(s) 429, 451, 619
BstMAI GTCTC 2 cut(s) 27, 194
BstMBI GATC 3 cut(s) 426, 448, 616
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNI CCWGG 2 cut(s) 573, 700
BstSCI CCNGG 2 cut(s) 571, 698
BstSFI CTRYAG 1 cut(s) 631
BstV1I GCAGC 3 cut(s) 155, 162, 203
BstX2I RGATCY 2 cut(s) 426, 616
BstYI RGATCY 2 cut(s) 426, 616
BstZ17I GTATAC 1 cut(s) 332
BsuRI GGCC 3 cut(s) 529, 608, 698
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 2 cut(s) 27, 365
BtsI GCAGTG 1 cut(s) 563
BtsIMutI CAGTG 1 cut(s) 563
Cac8I GCNNGC 2 cut(s) 189, 409
Cfr13I GGNCC 3 cut(s) 317, 527, 696
Csp6I GTAC 1 cut(s) 56
CviAII CATG 3 cut(s) 6, 443, 610
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 3 cut(s) 46, 134, 221
DpnI GATC 3 cut(s) 428, 450, 618
DpnII GATC 3 cut(s) 426, 448, 616
DraIII CACNNNGTG 1 cut(s) 679
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
Eam1104I CTCTTC 2 cut(s) 390, 719
EarI CTCTTC 2 cut(s) 390, 719
Eco130I CCWWGG 1 cut(s) 229
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 317
Eco57I CTGAAG 3 cut(s) 6, 405, 575
EcoNI CCTNNNNNAGG 2 cut(s) 340, 501
EcoO109I RGGNCCY 2 cut(s) 317, 527
EcoRII CCWGG 2 cut(s) 571, 698
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 3 cut(s) 9, 446, 613
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 1 cut(s) 716
FatI CATG 3 cut(s) 5, 442, 609
FblI GTMKAC 2 cut(s) 115, 331
Fnu4HI GCNGC 4 cut(s) 151, 169, 192, 213
FokI GGATG 2 cut(s) 14, 372
Fsp4HI GCNGC 4 cut(s) 151, 169, 192, 213
FspBI CTAG 1 cut(s) 230
GluI GCNGC 4 cut(s) 151, 169, 192, 213
HaeIII GGCC 3 cut(s) 529, 608, 698
HapII CCGG 1 cut(s) 518
Hin1II CATG 3 cut(s) 9, 446, 613
HinfI GANTC 2 cut(s) 71, 217
HpaII CCGG 1 cut(s) 518
HphI GGTGA 2 cut(s) 413, 501
Hpy166II GTNNAC 3 cut(s) 116, 332, 509
Hpy188I TCNGA 4 cut(s) 32, 342, 424, 594
Hpy188III TCNNGA 3 cut(s) 106, 430, 452
Hpy8I GTNNAC 3 cut(s) 116, 332, 509
HpyAV CCTTC 3 cut(s) 341, 429, 518
HpyCH4III ACNGT 3 cut(s) 244, 287, 513
HpyCH4V TGCA 7 cut(s) 191, 236, 282, 290, 365, 586, 633
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 3 cut(s) 46, 134, 221
Hsp92II CATG 3 cut(s) 9, 446, 613
Kzo9I GATC 3 cut(s) 426, 448, 616
Lsp1109I GCAGC 3 cut(s) 155, 162, 203
LweI GCATC 1 cut(s) 394
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 2 cut(s) 513, 575
MalI GATC 3 cut(s) 428, 450, 618
MboI GATC 3 cut(s) 426, 448, 616
MboII GAAGA 5 cut(s) 307, 407, 460, 533, 736
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 2 cut(s) 426, 616
MluCI AATT 6 cut(s) 10, 272, 310, 390, 557, 719
MmeI TCCRAC 2 cut(s) 58, 594
MnlI CCTC 5 cut(s) 156, 336, 391, 497, 540
MseI TTAA 3 cut(s) 173, 539, 546
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 2 cut(s) 573, 700
MunI CAATTG 1 cut(s) 390
MvaI CCWGG 2 cut(s) 573, 700
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeII GATC 3 cut(s) 426, 448, 616
NlaIII CATG 3 cut(s) 9, 446, 613
NlaIV GGNNCC 2 cut(s) 352, 618
NmuCI GTSAC 1 cut(s) 575
PfeI GAWTC 2 cut(s) 71, 217
PkrI GCNGC 4 cut(s) 152, 170, 193, 214
PpuMI RGGWCCY 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 571, 698
PspGI CCWGG 2 cut(s) 571, 698
PspN4I GGNNCC 2 cut(s) 352, 618
PspPI GGNCC 3 cut(s) 317, 527, 696
PspPPI RGGWCCY 1 cut(s) 317
PstI CTGCAG 1 cut(s) 635
PsuI RGATCY 2 cut(s) 426, 616
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
SaqAI TTAA 3 cut(s) 173, 539, 546
SatI GCNGC 4 cut(s) 151, 169, 192, 213
Sau3AI GATC 3 cut(s) 426, 448, 616
Sau96I GGNCC 3 cut(s) 317, 527, 696
ScrFI CCNGG 2 cut(s) 573, 700
SfaNI GCATC 1 cut(s) 394
SfcI CTRYAG 1 cut(s) 631
SinI GGWCC 1 cut(s) 317
SmlI CTYRAG 2 cut(s) 545, 602
SmoI CTYRAG 2 cut(s) 545, 602
Sse9I AATT 6 cut(s) 10, 272, 310, 390, 557, 719
SsiI CCGC 1 cut(s) 212
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 571, 698
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 3 cut(s) 244, 287, 513
TasI AATT 6 cut(s) 10, 272, 310, 390, 557, 719
TatI WGTACW 1 cut(s) 55
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 2 cut(s) 71, 217
Tru1I TTAA 3 cut(s) 173, 539, 546
Tru9I TTAA 3 cut(s) 173, 539, 546
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 3 cut(s) 150, 168, 191
Tsp45I GTSAC 1 cut(s) 575
TspRI CASTG 1 cut(s) 570
Vha464I CTTAAG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 317
XagI CCTNNNNNAGG 2 cut(s) 340, 501
XapI RAATTY 3 cut(s) 10, 272, 310
XmaJI CCTAGG 1 cut(s) 229
XmiI GTMKAC 2 cut(s) 115, 331
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.