Rorug05G0324400

DNA polymerase epsilon subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009988
Physical Location & Seq
Reverse (-)
38918367 .. 38919255
889 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0324400.1

Sequence Viewer

Length: 846 bp
ATGGCAATTCTCTCCAACAAACTCCCAGTCGGGGTCAGGTTCCTTCCCACTGACCACGAACTGCTCGATTACTATCTCCGCACAAGGATCGCCATGGGTGACCAGTTTCGCACCGATTTCGTCGCCGATTGTCCTCATTTTTACGGAGACAAGGAGCCCTGGGTTGTCTGGCAGATCTACGGCGGCGGTGAGTCCAAGAAAGTGGAGGATGAAAAGACTCTCTATTTTTTCACCCATCGGAGAAGGCTGAGTCCCACCGCCAAGAGATTTGATCGGAAAGTGGGTTCCGGCACTTGGAGTGGTCAGTATTCGAGAGAAGTTGAGGATGATGGTGTTGTTATTGGGCTCAAGCGGGAGTTCCGATACGAGGGCGGTTGTTACGAGAGTCACAACGACGCTTGGTTGATGCAAGAGTTTCAGCTTGATGATGATTCGGATTTGGTACTGTGCTCTCTGAGAAAGAACCCCAGAAAGGCTCAGGGGAGCAATGACAAAAAGAGGAGCATCGGGGATCAACCCACAAAGCCTAAATCTAAAAGGCAGAAAAAGGAAGGGAGTTGTGTTCATCAGGAACAAGGCAGCAGTACTGTTCATCCCAATTACGAGTTGCCCTATCCACAAATGGATGTTCATCCCAATCACGAGTTGCCCTATCCACAAATGGATGATCTAGATTTTTATTTTCCTGAGATTTATCCAGATCTGTTTGTTGATGATGGGCGTGATTTATTTACTATGGATGAATTGCTTGGACAAACCCCAGTTTGGGAGGAGGGCAACAACCAAACCCCAGTTTGGGAGGAGGGCAACAACTTCAATTTGGTTACAGGAGTACCACTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

281

Amino Acids

32.63

Weight (kDa)

5.1

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 141 4.8e-22 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 79, 183, 186, 258, 352, 372
AclWI GGATC 2 cut(s) 95, 519
AfaI GTAC 3 cut(s) 444, 586, 834
AfiI CCNNNNNNNGG 8 cut(s) 31, 294, 367, 472, 765, 766, 795, 796
AgsI TTSAA 1 cut(s) 817
AjnI CCWGG 1 cut(s) 158
AloI GAACNNNNNNTCC 2 cut(s) 268, 300
AluBI AGCT 1 cut(s) 421
AluI AGCT 1 cut(s) 421
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 95, 519
ApeKI GCWGC 1 cut(s) 579
ArsI GACNNNNNNTTYG 2 cut(s) 12, 44
AsuHPI GGTGA 3 cut(s) 110, 200, 223
BaeI ACNNNNGTAYC 1 cut(s) 816
BanII GRGCYC 2 cut(s) 159, 348
BauI CACGAG 1 cut(s) 641
Bbv12I GWGCWC 1 cut(s) 452
BbvI GCAGC 1 cut(s) 591
BccI CCATC 3 cut(s) 243, 323, 710
BceAI ACGGC 1 cut(s) 196
BcgI CGANNNNNNTGC 4 cut(s) 70, 100, 104, 134
BciT130I CCWGG 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 671
BglII AGATCT 2 cut(s) 174, 700
BisI GCNGC 2 cut(s) 184, 580
BlsI GCNGC 2 cut(s) 185, 581
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 1 cut(s) 160
BmiI GGNNCC 3 cut(s) 41, 156, 286
BmrFI CCNGG 1 cut(s) 160
BmrI ACTGGG 3 cut(s) 20, 755, 785
BmsI GCATC 2 cut(s) 396, 513
BmuI ACTGGG 3 cut(s) 20, 755, 785
Bpu10I CCTNAGC 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 332
BsaBI GATNNNNATC 2 cut(s) 72, 630
BsaJI CCNNGG 3 cut(s) 93, 158, 159
Bsc4I CCNNNNNNNGG 8 cut(s) 31, 294, 367, 472, 765, 766, 795, 796
Bse1I ACTGG 4 cut(s) 26, 103, 761, 791
Bse3DI GCAATG 1 cut(s) 493
Bse8I GATNNNNATC 2 cut(s) 72, 630
BseBI CCWGG 1 cut(s) 160
BseDI CCNNGG 3 cut(s) 93, 158, 159
BseGI GGATG 7 cut(s) 214, 331, 592, 631, 631, 670, 745
BseJI GATNNNNATC 2 cut(s) 72, 630
BseLI CCNNNNNNNGG 8 cut(s) 31, 294, 367, 472, 765, 766, 795, 796
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 4 cut(s) 239, 446, 491, 678
BseNI ACTGG 4 cut(s) 26, 103, 761, 791
BseRI GAGGAG 3 cut(s) 514, 785, 815
BseXI GCAGC 1 cut(s) 591
BsiHKAI GWGCWC 1 cut(s) 452
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 8 cut(s) 31, 294, 367, 472, 765, 766, 795, 796
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 237
Bsp1286I GDGCHC 3 cut(s) 159, 348, 452
Bsp143I GATC 6 cut(s) 87, 174, 271, 511, 667, 700
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 6 cut(s) 79, 183, 186, 258, 352, 372
BspCNI CTCAG 4 cut(s) 240, 447, 490, 679
BspLI GGNNCC 3 cut(s) 41, 156, 286
BspPI GGATC 2 cut(s) 95, 519
BsrDI GCAATG 1 cut(s) 493
BsrI ACTGG 4 cut(s) 26, 103, 761, 791
BssECI CCNNGG 3 cut(s) 93, 158, 159
BssMI GATC 6 cut(s) 87, 174, 271, 511, 667, 700
BssSI CACGAG 1 cut(s) 641
BssT1I CCWWGG 1 cut(s) 93
Bst2BI CACGAG 1 cut(s) 641
Bst2UI CCWGG 1 cut(s) 160
Bst4CI ACNGT 2 cut(s) 447, 589
BstDEI CTNAG 4 cut(s) 248, 455, 477, 687
BstDSI CCRYGG 1 cut(s) 93
BstEII GGTNACC 1 cut(s) 98
BstF5I GGATG 7 cut(s) 214, 331, 592, 631, 631, 670, 745
BstKTI GATC 6 cut(s) 90, 177, 274, 514, 670, 703
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 6 cut(s) 87, 174, 271, 511, 667, 700
BstNI CCWGG 1 cut(s) 160
BstPI GGTNACC 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 591
BstX2I RGATCY 2 cut(s) 174, 700
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 2 cut(s) 174, 700
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 7 cut(s) 214, 331, 592, 631, 631, 670, 745
BtsIMutI CAGTG 1 cut(s) 48
CseI GACGC 1 cut(s) 404
Csp6I GTAC 3 cut(s) 443, 585, 833
CviAII CATG 1 cut(s) 94
CviJI RGCY 6 cut(s) 157, 247, 346, 421, 476, 526
CviKI_1 RGCY 6 cut(s) 157, 247, 346, 421, 476, 526
CviQI GTAC 3 cut(s) 443, 585, 833
DdeI CTNAG 4 cut(s) 248, 455, 477, 687
DpnI GATC 6 cut(s) 89, 176, 273, 513, 669, 702
DpnII GATC 6 cut(s) 87, 174, 271, 511, 667, 700
Eco130I CCWWGG 1 cut(s) 93
Eco24I GRGCYC 2 cut(s) 159, 348
Eco91I GGTNACC 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 93
EcoT38I GRGCYC 2 cut(s) 159, 348
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 1 cut(s) 97
FaiI YATR 2 cut(s) 95, 737
FaqI GGGAC 1 cut(s) 237
FatI CATG 1 cut(s) 93
FauI CCCGC 1 cut(s) 345
Fnu4HI GCNGC 2 cut(s) 184, 580
FokI GGATG 7 cut(s) 221, 338, 579, 618, 638, 677, 752
FriOI GRGCYC 2 cut(s) 159, 348
Fsp4HI GCNGC 2 cut(s) 184, 580
FspBI CTAG 1 cut(s) 671
GluI GCNGC 2 cut(s) 184, 580
HapII CCGG 1 cut(s) 288
HgaI GACGC 1 cut(s) 404
Hin1II CATG 1 cut(s) 97
HinfI GANTC 5 cut(s) 191, 217, 250, 385, 431
HpaII CCGG 1 cut(s) 288
HphI GGTGA 3 cut(s) 110, 200, 223
Hpy188I TCNGA 5 cut(s) 240, 276, 362, 436, 456
Hpy188III TCNNGA 6 cut(s) 312, 569, 641, 671, 686, 698
Hpy99I CGWCG 2 cut(s) 125, 398
HpyAV CCTTC 3 cut(s) 53, 237, 545
HpyCH4III ACNGT 2 cut(s) 447, 589
HpyCH4V TGCA 1 cut(s) 409
HpyF3I CTNAG 4 cut(s) 248, 455, 477, 687
Hsp92II CATG 1 cut(s) 97
Kzo9I GATC 6 cut(s) 87, 174, 271, 511, 667, 700
LmnI GCTCC 3 cut(s) 154, 483, 501
Lsp1109I GCAGC 1 cut(s) 591
LweI GCATC 2 cut(s) 396, 513
MaeI CTAG 1 cut(s) 671
MaeIII GTNAC 4 cut(s) 98, 377, 386, 823
MalI GATC 6 cut(s) 89, 176, 273, 513, 669, 702
MboI GATC 6 cut(s) 87, 174, 271, 511, 667, 700
MflI RGATCY 2 cut(s) 174, 700
MhlI GDGCHC 3 cut(s) 159, 348, 452
MluCI AATT 4 cut(s) 6, 598, 743, 817
MlyI GAGTC 4 cut(s) 200, 211, 259, 394
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 9 cut(s) 144, 199, 316, 361, 492, 763, 766, 793, 796
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 160
MvaI CCWGG 1 cut(s) 160
NcoI CCATGG 1 cut(s) 93
NdeII GATC 6 cut(s) 87, 174, 271, 511, 667, 700
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 3 cut(s) 41, 156, 286
NmuCI GTSAC 2 cut(s) 98, 386
PasI CCCWGGG 1 cut(s) 159
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PfeI GAWTC 1 cut(s) 431
PkrI GCNGC 2 cut(s) 185, 581
PleI GAGTC 4 cut(s) 199, 211, 258, 393
PpsI GAGTC 4 cut(s) 199, 211, 258, 393
Psp6I CCWGG 1 cut(s) 158
PspEI GGTNACC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 158
PspN4I GGNNCC 3 cut(s) 41, 156, 286
PsuI RGATCY 2 cut(s) 174, 700
RsaI GTAC 3 cut(s) 444, 586, 834
RsaNI GTAC 3 cut(s) 443, 585, 833
SatI GCNGC 2 cut(s) 184, 580
Sau3AI GATC 6 cut(s) 87, 174, 271, 511, 667, 700
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 4 cut(s) 200, 211, 259, 394
ScrFI CCNGG 1 cut(s) 160
SduI GDGCHC 3 cut(s) 159, 348, 452
SetI ASST 2 cut(s) 41, 423
SfaNI GCATC 2 cut(s) 396, 513
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 4 cut(s) 6, 598, 743, 817
SsiI CCGC 6 cut(s) 79, 183, 186, 258, 352, 372
SspMI CTAG 1 cut(s) 671
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 447, 589
TaqI TCGA 2 cut(s) 66, 311
TasI AATT 4 cut(s) 6, 598, 743, 817
TatI WGTACW 1 cut(s) 584
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 1 cut(s) 431
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 2 cut(s) 98, 386
TseI GCWGC 1 cut(s) 579
Tsp45I GTSAC 2 cut(s) 98, 386
TspDTI ATGAA 5 cut(s) 225, 554, 581, 620, 756
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 1 cut(s) 55
XbaI TCTAGA 1 cut(s) 670
XspI CTAG 1 cut(s) 671
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.