Rw5G036680

DNA polymerase epsilon subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009988
Physical Location & Seq
Forward (+)
64341073 .. 64343592
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036680.1

Sequence Viewer

Length: 588 bp
ATGGCGACCCCCAAGAAAGGCACACCCCCAAAGAAAACCAAGGAAGCACAGAAGCCAAGCGCCAAAACCAGCAAGAACGACGGCAACAAAATAAAAAAGAAGACCAGTAACGGCACCGCTGCTGCGGCGGCGGCGGCTGTGAGATCCCCGAGCTCAAAGCCCCAGAAGAGCAGAGAGCAAAATGGGGGCAAAAGTACGAAAACACCCACCCCGAAATCCAAGAAGCCCCAGCAGAACGGAAAGCGAGTCCGGGAGGAAGAGGTGGAAGAGAAAGAGGATGTGAAGTCGTACACTTTTCCGATGGAGCGGGTTAAGAGGATTATCAGGGCCGAAGATTCCGACTTGCGCATCAGCTCCGACGCCGTTTTTCTCGTCAACAAGGCCACCGAGAAGTTCCTTGAGAAGTTTAGTGAAGATGCGCATAAATGTTGTGTTAAGGATCGCAAGAAGGCTCTTGCTTACAAGCACCTATCCTCAGTCGTTTGCAAACAAAAGAGATACGAGTTTCTTTCAGATTTTGTTCCTGAGAAAATAAAAGCTGCGGATGCATTGGCAAAGAGAAAACAAGCCCAAGAAGGAACAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

195

Amino Acids

21.7

Weight (kDa)

9.96

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 86 - 162 2.4e-11 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 98 - 161 8.3e-17 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 347, 420
AccB1I GGYRCC 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 307
AciI CCGC 7 cut(s) 117, 125, 128, 131, 134, 307, 542
AclWI GGATC 2 cut(s) 138, 447
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 2 cut(s) 196, 290
AfiI CCNNNNNNNGG 1 cut(s) 17
AluBI AGCT 4 cut(s) 153, 354, 539, 585
AluI AGCT 4 cut(s) 153, 354, 539, 585
Alw21I GWGCWC 1 cut(s) 155
AlwI GGATC 2 cut(s) 138, 447
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 2 cut(s) 327, 381
ApeKI GCWGC 3 cut(s) 119, 122, 539
AspLEI GCGC 3 cut(s) 62, 348, 421
AspS9I GGNCC 1 cut(s) 327
AsuC2I CCSGG 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 148
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 155
BbsI GAAGAC 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 106, 109, 526
BccI CCATC 1 cut(s) 295
BceAI ACGGC 3 cut(s) 97, 127, 347
BcnI CCSGG 1 cut(s) 251
BfaI CTAG 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 63
BisI GCNGC 7 cut(s) 120, 123, 126, 129, 132, 135, 540
BlsI GCNGC 7 cut(s) 121, 124, 127, 130, 133, 136, 541
Bme1390I CCNGG 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 327
BmiI GGNNCC 1 cut(s) 115
BmrFI CCNGG 1 cut(s) 251
BmsI GCATC 3 cut(s) 357, 406, 535
BpiI GAAGAC 1 cut(s) 107
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 360
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 17
Bse1I ACTGG 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 283, 550
BseLI CCNNNNNNNGG 1 cut(s) 17
BseMII CTCAG 2 cut(s) 489, 516
BseNI ACTGG 1 cut(s) 105
BseXI GCAGC 3 cut(s) 106, 109, 526
BseYI CCCAGC 1 cut(s) 228
BshFI GGCC 2 cut(s) 329, 383
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 1 cut(s) 250
BslI CCNNNNNNNGG 1 cut(s) 17
BsnI GGCC 2 cut(s) 329, 383
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 155
Bsp143I GATC 2 cut(s) 143, 439
BspACI CCGC 7 cut(s) 117, 125, 128, 131, 134, 307, 542
BspANI GGCC 2 cut(s) 329, 383
BspCNI CTCAG 2 cut(s) 488, 517
BspLI GGNNCC 1 cut(s) 115
BspPI GGATC 2 cut(s) 138, 447
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 307
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 2 cut(s) 143, 439
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 39
Bst6I CTCTTC 3 cut(s) 161, 252, 261
BstACI GRCGYC 1 cut(s) 360
BstDEI CTNAG 2 cut(s) 475, 525
BstF5I GGATG 2 cut(s) 283, 550
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 3 cut(s) 62, 348, 421
BstKTI GATC 2 cut(s) 146, 442
BstMBI GATC 2 cut(s) 143, 439
BstMWI GCNNNNNNNGC 5 cut(s) 125, 128, 131, 134, 545
BstSCI CCNGG 1 cut(s) 249
BstV1I GCAGC 3 cut(s) 106, 109, 526
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 2 cut(s) 329, 383
BtsCI GGATG 2 cut(s) 283, 550
CfoI GCGC 3 cut(s) 62, 348, 421
Cfr13I GGNCC 1 cut(s) 327
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 195, 289
CviQI GTAC 2 cut(s) 195, 289
DdeI CTNAG 2 cut(s) 475, 525
DpnI GATC 2 cut(s) 145, 441
DpnII GATC 2 cut(s) 143, 439
Eam1104I CTCTTC 3 cut(s) 161, 252, 261
EarI CTCTTC 3 cut(s) 161, 252, 261
Ecl136II GAGCTC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 155
Eco53kI GAGCTC 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 39
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 39
FaiI YATR 1 cut(s) 423
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 7 cut(s) 120, 123, 126, 129, 132, 135, 540
FokI GGATG 2 cut(s) 290, 557
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 7 cut(s) 120, 123, 126, 129, 132, 135, 540
FspAI RTGCGCAY 1 cut(s) 420
FspBI CTAG 1 cut(s) 586
FspI TGCGCA 2 cut(s) 347, 420
GlaI GCGC 3 cut(s) 61, 347, 420
GluI GCNGC 7 cut(s) 120, 123, 126, 129, 132, 135, 540
GsaI CCCAGC 1 cut(s) 232
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 2 cut(s) 329, 383
HapII CCGG 1 cut(s) 250
HgaI GACGC 1 cut(s) 368
HhaI GCGC 3 cut(s) 62, 348, 421
Hin1I GRCGYC 1 cut(s) 360
Hin6I GCGC 3 cut(s) 60, 346, 419
HinP1I GCGC 3 cut(s) 60, 346, 419
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 2 cut(s) 246, 335
HpaII CCGG 1 cut(s) 250
Hpy166II GTNNAC 2 cut(s) 291, 376
Hpy188I TCNGA 4 cut(s) 300, 340, 358, 514
Hpy188III TCNNGA 1 cut(s) 524
Hpy8I GTNNAC 2 cut(s) 291, 376
Hpy99I CGWCG 2 cut(s) 83, 362
HpyAV CCTTC 2 cut(s) 442, 569
HpyCH4V TGCA 2 cut(s) 486, 548
HpyF10VI GCNNNNNNNGC 5 cut(s) 125, 128, 131, 134, 545
HpyF3I CTNAG 2 cut(s) 475, 525
Hsp92I GRCGYC 1 cut(s) 360
HspAI GCGC 3 cut(s) 60, 346, 419
Kzo9I GATC 2 cut(s) 143, 439
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 2 cut(s) 304, 359
LpnPI CCDG 7 cut(s) 82, 118, 176, 242, 263, 310, 537
Lsp1109I GCAGC 3 cut(s) 106, 109, 526
LweI GCATC 3 cut(s) 357, 406, 535
MaeI CTAG 1 cut(s) 586
MaeIII GTNAC 1 cut(s) 107
MalI GATC 2 cut(s) 145, 441
MbiI CCGCTC 1 cut(s) 307
MboI GATC 2 cut(s) 143, 439
MboII GAAGA 6 cut(s) 112, 178, 269, 278, 344, 425
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 1 cut(s) 155
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 2 cut(s) 363, 381
MnlI CCTC 5 cut(s) 247, 253, 268, 309, 484
Mph1103I ATGCAT 1 cut(s) 550
MseI TTAA 2 cut(s) 312, 435
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 1 cut(s) 250
MspR9I CCNGG 1 cut(s) 251
MwoI GCNNNNNNNGC 5 cut(s) 125, 128, 131, 134, 545
NciI CCSGG 1 cut(s) 251
NdeII GATC 2 cut(s) 143, 439
NlaIV GGNNCC 1 cut(s) 115
NsbI TGCGCA 2 cut(s) 347, 420
NsiI ATGCAT 1 cut(s) 550
PciSI GCTCTTC 1 cut(s) 161
PfeI GAWTC 1 cut(s) 335
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 7 cut(s) 121, 124, 127, 130, 133, 136, 541
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 228
PspN4I GGNNCC 1 cut(s) 115
PspPI GGNCC 1 cut(s) 327
PsuI RGATCY 1 cut(s) 143
RsaI GTAC 2 cut(s) 196, 290
RsaNI GTAC 2 cut(s) 195, 289
SacI GAGCTC 1 cut(s) 155
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 2 cut(s) 312, 435
SatI GCNGC 7 cut(s) 120, 123, 126, 129, 132, 135, 540
Sau3AI GATC 2 cut(s) 143, 439
Sau96I GGNCC 1 cut(s) 327
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 1 cut(s) 251
SduI GDGCHC 1 cut(s) 155
SetI ASST 6 cut(s) 155, 264, 356, 471, 541, 587
SfaNI GCATC 3 cut(s) 357, 406, 535
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
SsiI CCGC 7 cut(s) 117, 125, 128, 131, 134, 307, 542
SspMI CTAG 1 cut(s) 586
SstI GAGCTC 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 1 cut(s) 39
TauI GCSGC 4 cut(s) 128, 131, 134, 137
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 2 cut(s) 312, 435
Tru9I TTAA 2 cut(s) 312, 435
TseI GCWGC 3 cut(s) 119, 122, 539
TspGWI ACGGA 1 cut(s) 252
XspI CTAG 1 cut(s) 586
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.