Prupe.6G103900_v2.0.a1
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0010650
Physical Location & Seq
Reverse (-)
7292783 .. 7297414
4632 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G103900.1

Sequence Viewer

Length: 666 bp
ATGCGCCTCTCTCTCTTTCTGTGTGGAAGTGAATCGATCCCGGGTCCCCCTGTTTGTGTGTTCTGTGCAAGTAGCCCCAGGAAGAAAAGCAGTTATAAGGAGGCAGCTATTGAGCTTGGAGAAGAATTGGTTTCAAAGAACATTGATTTGGTTTATGGAGGAGGGAGTGTTGGCTTGATGGGGTTGATTTCCCAAGCTGTTTATGATGGTGGTAGACATGTCATTGGAGTTATCCCCAAGACACTCATGCCTAGAGAGATAACTGGAGAAACAGTGGGGGAAGTGAAGGCAGTAGCAGACATGCACCAAAGGAAGGCAGAGATGGCTAAGCACTCAGATGCCTTTATTGCCTTACCTGGTGGCTATGGGACTCTTGAAGAACTTCTTGAAGTGATCACATGGGCCCAGCTGGGAATCCATGATAAACCTGTGGGATTGTTGAATGTGGACGGGTTTTATAACTCATTGTTGTCGTTTATTGACAAGGCTGTTGAAGAAGGTTTCATTAGTCCAACTGCACGACACATAATCGTTTCTGCCCCTACTGCTAAGGAGCTAGTGAAGAAAATGGAGGAATATTTCCCACGCCATGAAAGAGTTGCTTCAAAGCTAAGCTGGGAAATTGAACAGCTAAGCTACCCCACAATATGTGACATTTCAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

222

Amino Acids

24.13

Weight (kDa)

5.72

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28305 AT2G28305 AT2G28305
fragaria_vesca FvH4_3g03400 FvH4_3g03400 FvH4_3g31870 FvH4_3g31870 FvH4_3g31870 FvH4_3g31870
malus_domestica MD03G1120500.v1.1 MD11G1138900.v1.1
prunus_persica Prupe.6G103900_v2.0.a1
pyrus_communis pycom11g11460
rosa_chinensis RchiOBHm_Chr5g0058621
rosa_multiflora Rmu_sc0000367.1_g000164
rosa_roxburghii Rroxscaffold_1G00021850
rosa_rugosa Rorug05G0316500
rosa_samantha Rh5AG382500 Rh5DG409500
rosa_wichuraiana Rw5G036020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 96, 459
AccI GTMKAC 1 cut(s) 214
AclWI GGATC 1 cut(s) 31
AfiI CCNNNNNNNGG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 217
AgsI TTSAA 8 cut(s) 135, 377, 389, 442, 494, 606, 626, 660
AjnI CCWGG 2 cut(s) 77, 355
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 9 cut(s) 107, 115, 197, 409, 556, 610, 615, 631, 636
AluI AGCT 9 cut(s) 107, 115, 197, 409, 556, 610, 615, 631, 636
AlwI GGATC 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 40
AoxI GGCC 1 cut(s) 402
ApaI GGGCCC 1 cut(s) 406
ApeKI GCWGC 1 cut(s) 104
Asp700I GAANNNNTTC 1 cut(s) 381
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 3 cut(s) 44, 402, 403
AsuC2I CCSGG 2 cut(s) 41, 42
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 1 cut(s) 44
BaeGI GKGCMC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 406
BbvI GCAGC 1 cut(s) 116
BccI CCATC 3 cut(s) 172, 200, 316
BciT130I CCWGG 2 cut(s) 79, 357
BclI TGATCA 1 cut(s) 393
BcnI CCSGG 2 cut(s) 41, 42
BfaI CTAG 2 cut(s) 252, 557
BisI GCNGC 1 cut(s) 105
BlpI GCTNAGC 3 cut(s) 327, 611, 632
BlsI GCNGC 1 cut(s) 106
Bme1390I CCNGG 4 cut(s) 41, 42, 79, 357
Bme18I GGWCC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 40
BmgT120I GGNCC 3 cut(s) 44, 402, 403
BmiI GGNNCC 3 cut(s) 45, 46, 404
BmrFI CCNGG 4 cut(s) 41, 42, 79, 357
BmsI GCATC 1 cut(s) 328
BpmI CTGGAG 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 549
Bpu1102I GCTNAGC 3 cut(s) 327, 611, 632
BpuMI CCSGG 2 cut(s) 41, 42
Bsa29I ATCGAT 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 40, 77
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 258, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 268
BseBI CCWGG 2 cut(s) 79, 357
BseCI ATCGAT 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 40, 77
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 406
BseXI GCAGC 1 cut(s) 116
BseYI CCCAGC 3 cut(s) 405, 409, 615
BsgI GTGCAG 1 cut(s) 501
BshFI GGCC 1 cut(s) 404
BshVI ATCGAT 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 41
BslFI GGGAC 2 cut(s) 30, 382
BslI CCNNNNNNNGG 1 cut(s) 313
BsmFI GGGAC 2 cut(s) 30, 382
BsnI GGCC 1 cut(s) 404
BsoBI CYCGRG 1 cut(s) 40
Bsp120I GGGCCC 1 cut(s) 402
Bsp1286I GDGCHC 1 cut(s) 406
Bsp143I GATC 2 cut(s) 36, 393
Bsp1720I GCTNAGC 3 cut(s) 327, 611, 632
BspANI GGCC 1 cut(s) 404
BspCNI CTCAG 1 cut(s) 347
BspDI ATCGAT 1 cut(s) 35
BspLI GGNNCC 3 cut(s) 45, 46, 404
BspPI GGATC 1 cut(s) 31
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 2 cut(s) 40, 77
BssMI GATC 2 cut(s) 36, 393
Bst2UI CCWGG 2 cut(s) 79, 357
Bst4CI ACNGT 1 cut(s) 274
BstDEI CTNAG 5 cut(s) 327, 334, 549, 611, 632
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 2 cut(s) 39, 396
BstMBI GATC 2 cut(s) 36, 393
BstMWI GCNNNNNNNGC 3 cut(s) 323, 347, 545
BstNI CCWGG 2 cut(s) 79, 357
BstNSI RCATGY 2 cut(s) 221, 304
BstSCI CCNGG 4 cut(s) 39, 40, 77, 355
BstSLI GKGCMC 1 cut(s) 406
BstV1I GCAGC 1 cut(s) 116
Bsu15I ATCGAT 1 cut(s) 35
BsuRI GGCC 1 cut(s) 404
BsuTUI ATCGAT 1 cut(s) 35
BtsIMutI CAGTG 1 cut(s) 279
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 3 cut(s) 44, 402, 403
Cfr9I CCCGGG 1 cut(s) 40
ClaI ATCGAT 1 cut(s) 35
CsiI ACCWGGT 1 cut(s) 355
CviAII CATG 6 cut(s) 218, 247, 301, 399, 419, 590
DdeI CTNAG 5 cut(s) 327, 334, 549, 611, 632
DpnI GATC 2 cut(s) 38, 395
DpnII GATC 2 cut(s) 36, 393
Eco24I GRGCYC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 40
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 2 cut(s) 77, 355
EcoT38I GRGCYC 1 cut(s) 406
FaeI CATG 6 cut(s) 221, 250, 304, 402, 422, 593
FalI AAGNNNNNCTT 4 cut(s) 369, 401, 586, 618
FaqI GGGAC 2 cut(s) 30, 382
FatI CATG 6 cut(s) 217, 246, 300, 398, 418, 589
FbaI TGATCA 1 cut(s) 393
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 1 cut(s) 105
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 2 cut(s) 252, 557
GlaI GCGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 105
GsaI CCCAGC 3 cut(s) 409, 413, 619
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 1 cut(s) 404
HapII CCGG 1 cut(s) 41
HhaI GCGC 1 cut(s) 6
Hin1II CATG 6 cut(s) 221, 250, 304, 402, 422, 593
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 3 cut(s) 32, 370, 414
HpaII CCGG 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 215, 448
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 3 cut(s) 374, 386, 660
Hpy8I GTNNAC 2 cut(s) 215, 448
HpyAV CCTTC 3 cut(s) 280, 307, 491
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4V TGCA 3 cut(s) 68, 304, 518
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 347, 545
HpyF3I CTNAG 5 cut(s) 327, 334, 549, 611, 632
Hsp92II CATG 6 cut(s) 221, 250, 304, 402, 422, 593
HspAI GCGC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 44
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 2 cut(s) 36, 393
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 1 cut(s) 116
LweI GCATC 1 cut(s) 328
MabI ACCWGGT 1 cut(s) 355
MaeI CTAG 2 cut(s) 252, 557
MaeIII GTNAC 1 cut(s) 650
MalI GATC 2 cut(s) 38, 395
MboI GATC 2 cut(s) 36, 393
MboII GAAGA 5 cut(s) 94, 134, 389, 506, 574
MhlI GDGCHC 1 cut(s) 406
MluCI AATT 2 cut(s) 125, 621
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 536
MnlI CCTC 5 cut(s) 17, 94, 152, 155, 565
MroXI GAANNNNTTC 1 cut(s) 381
MslI CAYNNNNRTG 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 4 cut(s) 41, 42, 79, 357
MvaI CCWGG 2 cut(s) 79, 357
MwoI GCNNNNNNNGC 3 cut(s) 323, 347, 545
NciI CCSGG 2 cut(s) 41, 42
NdeII GATC 2 cut(s) 36, 393
NlaIII CATG 6 cut(s) 221, 250, 304, 402, 422, 593
NlaIV GGNNCC 3 cut(s) 45, 46, 404
NmuCI GTSAC 1 cut(s) 650
NspI RCATGY 2 cut(s) 221, 304
PciI ACATGT 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 2 cut(s) 32, 414
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 44
PscI ACATGT 1 cut(s) 217
PsiI TTATAA 2 cut(s) 96, 459
Psp5II RGGWCCY 1 cut(s) 44
Psp6I CCWGG 2 cut(s) 77, 355
PspFI CCCAGC 3 cut(s) 405, 409, 615
PspGI CCWGG 2 cut(s) 77, 355
PspN4I GGNNCC 3 cut(s) 45, 46, 404
PspOMI GGGCCC 1 cut(s) 402
PspPI GGNCC 3 cut(s) 44, 402, 403
PspPPI RGGWCCY 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 336
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 2 cut(s) 36, 393
Sau96I GGNCC 3 cut(s) 44, 402, 403
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 4 cut(s) 41, 42, 79, 357
SduI GDGCHC 1 cut(s) 406
SexAI ACCWGGT 1 cut(s) 355
SfaNI GCATC 1 cut(s) 328
SinI GGWCC 1 cut(s) 44
SmaI CCCGGG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 336
Sse9I AATT 2 cut(s) 125, 621
SspI AATATT 1 cut(s) 578
SspMI CTAG 2 cut(s) 252, 557
StyD4I CCNGG 4 cut(s) 39, 40, 77, 355
TaaI ACNGT 1 cut(s) 274
TaqI TCGA 1 cut(s) 35
TasI AATT 2 cut(s) 125, 621
TfiI GAWTC 2 cut(s) 32, 414
TscAI CASTG 1 cut(s) 279
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 1 cut(s) 104
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 2 cut(s) 493, 606
TspMI CCCGGG 1 cut(s) 40
TspRI CASTG 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 44
XceI RCATGY 2 cut(s) 221, 304
XmaI CCCGGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 214
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 2 cut(s) 252, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.