Rorug05G0316500
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010650
Physical Location & Seq
Reverse (-)
37627498 .. 37630797
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0316500.1

Sequence Viewer

Length: 3300 bp
ATGGCTGAAGTTGTGGGTGGAGCTTTTCTCTCTTCTGCACTTGCTGTTCTGTTTGATAGGATGGCTTCCCGTCCGGTTGTTGACTTCATCCGAGGAAAGAAGACCACCGCTGGGCTGCTCCACAAGTTGAAGATTAAGTTGTTGTCGGTTAATAAGTTGCTCGATGATGCAGAGGAGAAGCAACTCAGAAACTCTAGAGTCAGGGATTGGCTTAATGAGCTTAAAGATGTTCTCTATCATGCCGATGACCTGTTGGATGAAATCAAGACTGAAGCTCTGCGTTGCAAGATGGAACAAGAAGATTCTGGAAGCAGCAGCACAAATCAGGTACTCAAGTTTAGTTCTATTTCCATTCATGAACTTGACGAAAGTCTGGAACCCAGGATTCTGGAGATTCTTGAAAGATTAGAACTGATTGTCAACGAAAAAGATGTGCTTGATTTGAAAGAGGGTGTTAAGGATAGACCACAAGGAAGACTGCCTACAACTTCTTTGGTGGAAGAGTCTAGTGTATATGGAAGGGATGAAGAAAAGGAGGCCATTATCAAGTTATTGTTAGCGGATGGTATGACTGGAAATAAGATAGATGTGCTTCCAATTGTGGGCATGGGTGGGCTTGGAAAGACCACTCTTGCTGAACTCATATACAACGATGACAGTGTCAAGCAGCATTTTGTCCATCAAGCATGGTCTTGTGTTTCAGTAGAATTTGATGTTATCAAAATAACACAGACACTTTATGGGGCGGTCACTTCACAAACTTGTATTATCACAGACCTAAACATGCTTCAAGTTAAACTAAAGGAGACGTTGTCAGGGAAGAAATTTCTCTTTGTTCTTGATGATGTTTGGATTGTGACTAATGACGAGTGGGATCTCTTAAGCCGACCCTTTGAGGGTGGAGGTCATGGAAGTAAGATCATTGTCACGACACGTCATGAAGATGTTGCAGGCATTATAGGTACCCTTCAAAGTCAACATCTAATGCAACTATCTGAGGAAAATTGCTGGTCGTTGTTCTCAAAACATGCCTTCAAGAATGCAGGTGCCACTGCAGATCCGTTGCTTGAAGTGATTGGAAAACAGATTGCTCAAAAGTGTAAAGGACTCCCTTTAGCTGCAAAATCACTTGGGGGTCTTTTATGTTCTGAACCAAATATTGAGAGATGGGAAGAAGTATTGAACAATGACATATGGGAGTTGCAAGATAAAAAGAATGGCATTTTGCCAGCTCTATGGTTGAGCTACCATTATCTACCTCCACATCTGAAGCGCTGTTTTGCTTACTGTTCCATATTTCCTCAAGATCATATGTTTCACAAACCAGATCTGGTTTTGTTGTGGATGGCTGAAGATCTCTTACCGTCGAAATCGAAGACTACATTTGAGGAAGTCGGAGGGAACTACTTTGATGATCTAGTGTCAAGGTCGTTTTTTCAACGTGTACTATATCAGCACTTCACAATGCATGACCTCATTCATGACTTGGCAAACTTTGTCTCCGGAGAGTTTTGTGTTAGGTTGGAGGACAATGACTCGGTGCTGGACAACAAAAGCAAGAGTCGCCACTTTTTTTGTGTGACCCCCGGTTTTAATCAATGGGACAGTTTTTATGAAGCCAAGTATTTGCGGACCTTTCTTGTACCAGATTCGATATCCTTCCAATGCTCCAGAGGTCCTGACTTAGATAATGATATTCTCTTGAAACTCACGGGTTTAAGAGTGCTCAAATTATTAGGGTACAATATTGGTGAGCTGCCTAGTTCAATTAGCAAGTTGAAGCATCTAAGGCACTTGGACTTGTCTGGCACATCAATTAAAAAGTTACCTGATGAAATGTGTACTTTATATAATTTACAGACTTTATTGTTGTTGGGTTGTGAATCTCTAGTTGAGTTGCCAACTGATTTGGGCAGATTAATAAACTTGCGGCATCTTGATATTAGACACACAAAGATAAAAAAGATGCCCCCAAGGATGGGTAAAATGAAAGATCTCCAAACATTAAGAGGTCAGTTTGCCCTAGACAAGTGCACTGGTGATAACATTGTCGAGATTAAAGAGCTTCAGCAGTTGCGCGGAACACTCTGTATCTCGGGGCTTCGCAATAGTGTGCATGTCACAGATGCCATTATGAGAAAGAAGAAGCATCTGGATGAATTAGTTTTGGAATGGGGAGGCAAGTCTGGTGACACAGACGTCGATAGACAAAAAGAGAGAGATCTGCTGGAGAACCTCCAACCTCATACAAACCTAAAAGAACTCACCATTCAATCTTATGGGGGCACAAGATTTCCAGGTTGGTTGGAAGATCATTCTAGTTTATCTAATCTGGTTCGCCTTCAACTTGTGGATTGTGGAAATTGTTGGTCGTTGCTATCAGTTGAGCAGCTACCCTCCCTCCAAGAGCTTGAGATTGAAGGATTTAATGGAGCGGTGACTGTGGGTTCTAAGCCTTCTCCTAATCTTCGTAAGCTTAGTCTGAGGTCTTGTCCCGAGGTAACCGGGACATTCATGTTAGCCTGCTTCCCAAAGCTTGAAATACTCAAATTGAAGTATGTTAATGTAGAATCTCTTGATTCCCCTTCACTCCTCGCCCTAGTCAAACTTTCAATATTTTATTGCCCAAATTTTGTTGGTTTTCGCGATGGAGGGCTGGATGCGCCCAACTTGATGATCTTAAAAGTCGAAACATGTCCGAAACTGAGGTCATTGCCGCAACACATGCACAACCTTCTCCCATCTCTGAGCTACTTGTGGCTAGTTGATTGTCCAGAATTGGAATCATTTCCCGAAGGGGGATTGCCGTCTAAGTTGGAAGACTTGATCATCCTGCGTTGCAAGAAACTCCTCATTGGAAACCGTATGCAGTGGGGTCTACCAACACTCACCTCTCTCAAATTCTTGACCGTCGACTTTGAGGGATGCGAACAAGTTGCAGATTCATTTCCAGACGAGGGGCTGCTGCCCACCACTCTCGGAAAACTCCGCATCTACAGTATTTCGAAGCTAGACGGCAAGGGGTTTAGACAACTCACCTCTCTTAAAGAGTTGTCAATTCGCAATTGCCCTGAACTCCGGTGCTTGCCAGATGAAGGGCTACCCACTTCTCTTTCTTGGCTGGACATCTATGATTGTCCTTTACTAGAACAACGTTGCCAGAGAGAGACAGGGGAAGATTGGCCCAAGATTGCTCACATCAAGACCATTCGAATCAATCACATAGTTATACGACGTGCAATGGTAATTATCCAATTCTCTATTCTGGTACGGTTTAGTTATACAATATCATTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

1099

Amino Acids

124.35

Weight (kDa)

5.99

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 101 3.8e-18 Rx N-terminal domain
NB-ARC PF00931 176 - 347 6.1e-34 NB-ARC domain
WHD_DRP PF23559 432 - 497 3e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 556 - 624 3.9e-11 Leucine-rich repeat region
LRR_14 PF23598 570 - 860 3e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 683 - 808 5.7e-35 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28305 AT2G28305 AT2G28305
fragaria_vesca FvH4_3g03400 FvH4_3g03400 FvH4_3g31870 FvH4_3g31870 FvH4_3g31870 FvH4_3g31870
malus_domestica MD03G1120500.v1.1 MD11G1138900.v1.1
prunus_persica Prupe.6G103900_v2.0.a1
pyrus_communis pycom11g11460
rosa_chinensis RchiOBHm_Chr5g0058621
rosa_multiflora Rmu_sc0000367.1_g000164
rosa_roxburghii Rroxscaffold_1G00021850
rosa_rugosa Rorug05G0316500
rosa_samantha Rh5AG382500 Rh5DG409500
rosa_wichuraiana Rw5G036020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1034
AasI GACNNNNNNGTC 1 cut(s) 2198
AatII GACGTC 1 cut(s) 2202
Acc36I ACCTGC 1 cut(s) 1034
Acc65I GGTACC 1 cut(s) 962
AccB1I GGYRCC 2 cut(s) 962, 1046
AccBSI CCGCTC 1 cut(s) 2435
AccI GTMKAC 2 cut(s) 2878, 2913
AccII CGCG 2 cut(s) 2079, 2646
AccIII TCCGGA 1 cut(s) 1502
AciI CCGC 9 cut(s) 108, 560, 746, 1630, 1930, 2079, 2435, 2717, 2989
AclI AACGTT 1 cut(s) 3154
AclWI GGATC 2 cut(s) 882, 1052
AcsI RAATTY 4 cut(s) 707, 824, 2629, 2900
AcuI CTGAAG 5 cut(s) 27, 291, 1289, 1371, 2051
AcyI GRCGYC 1 cut(s) 2199
AfaI GTAC 7 cut(s) 330, 964, 1446, 1644, 1742, 1843, 3270
AfeI AGCGCT 1 cut(s) 1274
AfiI CCNNNNNNNGG 8 cut(s) 111, 602, 896, 1978, 1979, 2798, 2957, 3095
AflII CTTAAG 1 cut(s) 880
AflIII ACRYGT 3 cut(s) 932, 1441, 2693
AjiI CACGTC 2 cut(s) 935, 3236
AjnI CCWGG 2 cut(s) 380, 2296
AjuI GAANNNNNNNTTGG 2 cut(s) 3246, 3278
Alw21I GWGCWC 2 cut(s) 1728, 2036
Alw26I GTCTC 3 cut(s) 800, 1504, 3161
Alw44I GTGCAC 1 cut(s) 2032
AlwI GGATC 2 cut(s) 882, 1052
Ama87I CYCGRG 2 cut(s) 2095, 2495
Aor13HI TCCGGA 1 cut(s) 1502
Aor51HI AGCGCT 1 cut(s) 1274
AoxI GGCC 2 cut(s) 537, 3182
ApaLI GTGCAC 1 cut(s) 2032
ApeKI GCWGC 9 cut(s) 115, 312, 315, 667, 1118, 1756, 2389, 2962, 2965
ApoI RAATTY 4 cut(s) 707, 824, 2629, 2900
ArsI GACNNNNNNTTYG 2 cut(s) 2693, 2725
AseI ATTAAT 1 cut(s) 1919
Asp700I GAANNNNTTC 1 cut(s) 2787
Asp718I GGTACC 1 cut(s) 962
AspLEI GCGC 3 cut(s) 1275, 2079, 2665
AspS9I GGNCC 3 cut(s) 1632, 1676, 3183
AsuC2I CCSGG 2 cut(s) 1587, 2506
AsuHPI GGTGA 7 cut(s) 1763, 2051, 2201, 2257, 2449, 2881, 3028
AsuII TTCGAA 2 cut(s) 3005, 3211
AvaI CYCGRG 2 cut(s) 2095, 2495
AvaII GGWCC 2 cut(s) 1632, 1676
BaeGI GKGCMC 2 cut(s) 2036, 2288
BaeI ACNNNNGTAYC 2 cut(s) 320, 353
BanI GGYRCC 2 cut(s) 962, 1046
BarI GAAGNNNNNNTAC 4 cut(s) 466, 498, 1344, 1376
BbsI GAAGAC 4 cut(s) 107, 481, 1382, 2826
Bbv12I GWGCWC 2 cut(s) 1728, 2036
BbvI GCAGC 9 cut(s) 102, 324, 327, 679, 1105, 1743, 2401, 2949, 2952
BccI CCATC 9 cut(s) 55, 283, 557, 687, 1161, 1339, 1972, 2642, 2749
BceAI ACGGC 2 cut(s) 2791, 3031
BcgI CGANNNNNNTGC 2 cut(s) 2918, 2952
BciT130I CCWGG 2 cut(s) 382, 2298
BclI TGATCA 1 cut(s) 2826
BcnI CCSGG 2 cut(s) 1587, 2506
BcoDI GTCTC 3 cut(s) 800, 1504, 3161
BfmI CTRYAG 2 cut(s) 1053, 2995
BfoI RGCGCY 1 cut(s) 1276
BfrI CTTAAG 1 cut(s) 880
BfuAI ACCTGC 1 cut(s) 1034
BglII AGATCT 4 cut(s) 1327, 1354, 1993, 2221
Bme1390I CCNGG 4 cut(s) 382, 1587, 2298, 2506
Bme18I GGWCC 2 cut(s) 1632, 1676
BmeT110I CYCGRG 2 cut(s) 2095, 2495
BmgBI CACGTC 2 cut(s) 935, 3236
BmgT120I GGNCC 3 cut(s) 1632, 1676, 3183
BmiI GGNNCC 3 cut(s) 378, 964, 1048
BmrFI CCNGG 4 cut(s) 382, 1587, 2298, 2506
BmsI GCATC 9 cut(s) 157, 1792, 1942, 1956, 2116, 2158, 2650, 2915, 3000
BoxI GACNNNNGTC 1 cut(s) 369
BpiI GAAGAC 4 cut(s) 107, 481, 1382, 2826
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BpmI CTGGAG 3 cut(s) 410, 1654, 2249
Bpu14I TTCGAA 2 cut(s) 3005, 3211
BpuEI CTTGAG 3 cut(s) 317, 1287, 2432
BpuMI CCSGG 2 cut(s) 1587, 2506
BsaHI GRCGYC 1 cut(s) 2199
BsaJI CCNNGG 5 cut(s) 91, 380, 1585, 1973, 2496
BsaWI WCCGGW 3 cut(s) 73, 1502, 3078
BsaXI ACNNNNNCTCC 6 cut(s) 797, 827, 1484, 1514, 2385, 2415
Bsc4I CCNNNNNNNGG 8 cut(s) 111, 602, 896, 1978, 1979, 2798, 2957, 3095
Bse1I ACTGG 2 cut(s) 577, 2041
Bse3DI GCAATG 2 cut(s) 2711, 3246
BseAI TCCGGA 1 cut(s) 1502
BseBI CCWGG 2 cut(s) 382, 2298
BseDI CCNNGG 5 cut(s) 91, 380, 1585, 1973, 2496
BseLI CCNNNNNNNGG 8 cut(s) 111, 602, 896, 1978, 1979, 2798, 2957, 3095
BseMI GCAATG 2 cut(s) 2711, 3246
BseMII CTCAG 5 cut(s) 199, 987, 2474, 2696, 2738
BseNI ACTGG 2 cut(s) 577, 2041
BseRI GAGGAG 3 cut(s) 188, 2582, 2840
BseSI GKGCMC 2 cut(s) 2036, 2288
BseXI GCAGC 9 cut(s) 102, 324, 327, 679, 1105, 1743, 2401, 2949, 2952
BseYI CCCAGC 1 cut(s) 110
BsgI GTGCAG 1 cut(s) 21
Bsh1236I CGCG 2 cut(s) 2079, 2646
BshFI GGCC 2 cut(s) 539, 3184
BshNI GGYRCC 2 cut(s) 962, 1046
BsiHKAI GWGCWC 2 cut(s) 1728, 2036
BsiHKCI CYCGRG 2 cut(s) 2095, 2495
BsiSI CCGG 5 cut(s) 74, 1503, 1587, 2505, 3079
BslFI GGGAC 3 cut(s) 1616, 2478, 2521
BslI CCNNNNNNNGG 8 cut(s) 111, 602, 896, 1978, 1979, 2798, 2957, 3095
BsmAI GTCTC 3 cut(s) 800, 1504, 3161
BsmBI CGTCTC 1 cut(s) 800
BsmFI GGGAC 3 cut(s) 1616, 2478, 2521
BsmI GAATGC 1 cut(s) 1045
BsnI GGCC 2 cut(s) 539, 3184
BsoBI CYCGRG 2 cut(s) 2095, 2495
Bsp119I TTCGAA 2 cut(s) 3005, 3211
Bsp1286I GDGCHC 3 cut(s) 1728, 2036, 2288
Bsp13I TCCGGA 1 cut(s) 1502
Bsp68I TCGCGA 1 cut(s) 2646
BspACI CCGC 9 cut(s) 108, 560, 746, 1630, 1930, 2079, 2435, 2717, 2989
BspANI GGCC 2 cut(s) 539, 3184
BspCNI CTCAG 5 cut(s) 198, 988, 2475, 2697, 2739
BspEI TCCGGA 1 cut(s) 1502
BspFNI CGCG 2 cut(s) 2079, 2646
BspHI TCATGA 3 cut(s) 355, 937, 1480
BspLI GGNNCC 3 cut(s) 378, 964, 1048
BspMAI CTGCAG 1 cut(s) 1057
BspMI ACCTGC 1 cut(s) 1034
BspPI GGATC 2 cut(s) 882, 1052
BspT104I TTCGAA 2 cut(s) 3005, 3211
BspT107I GGYRCC 2 cut(s) 962, 1046
BspTI CTTAAG 1 cut(s) 880
BsrBI CCGCTC 1 cut(s) 2435
BsrDI GCAATG 2 cut(s) 2711, 3246
BsrI ACTGG 2 cut(s) 577, 2041
BssECI CCNNGG 5 cut(s) 91, 380, 1585, 1973, 2496
BssNI GRCGYC 1 cut(s) 2199
BssT1I CCWWGG 1 cut(s) 1973
Bst2UI CCWGG 2 cut(s) 382, 2298
Bst4CI ACNGT 9 cut(s) 659, 1289, 1365, 1607, 2443, 2864, 2911, 2999, 3273
Bst6I CTCTTC 2 cut(s) 37, 495
BstACI GRCGYC 1 cut(s) 2199
BstAFI CTTAAG 1 cut(s) 880
BstAPI GCANNNNNTGC 1 cut(s) 2725
BstBI TTCGAA 2 cut(s) 3005, 3211
BstC8I GCNNGC 4 cut(s) 952, 1230, 2524, 3086
BstEII GGTNACC 1 cut(s) 2500
BstFNI CGCG 2 cut(s) 2079, 2646
BstH2I RGCGCY 1 cut(s) 1276
BstHHI GCGC 3 cut(s) 1275, 2079, 2665
BstMAI GTCTC 3 cut(s) 800, 1504, 3161
BstMWI GCNNNNNNNGC 5 cut(s) 217, 1563, 1789, 2662, 2725
BstNI CCWGG 2 cut(s) 382, 2298
BstNSI RCATGY 5 cut(s) 787, 1031, 2120, 2697, 2728
BstPAI GACNNNNGTC 1 cut(s) 369
BstPI GGTNACC 1 cut(s) 2500
BstSCI CCNGG 4 cut(s) 380, 1585, 2296, 2504
BstSFI CTRYAG 2 cut(s) 1053, 2995
BstSLI GKGCMC 2 cut(s) 2036, 2288
BstUI CGCG 2 cut(s) 2079, 2646
BstV1I GCAGC 9 cut(s) 102, 324, 327, 679, 1105, 1743, 2401, 2949, 2952
BstV2I GAAGAC 4 cut(s) 107, 481, 1382, 2826
BstX2I RGATCY 6 cut(s) 874, 1057, 1327, 1354, 1993, 2221
BstXI CCANNNNNNTGG 2 cut(s) 388, 1236
BstYI RGATCY 6 cut(s) 874, 1057, 1327, 1354, 1993, 2221
BsuRI GGCC 2 cut(s) 539, 3184
BtgZI GCGATG 1 cut(s) 2661
BtrI CACGTC 2 cut(s) 935, 3236
BtsI GCAGTG 2 cut(s) 1050, 2876
BtsIMutI CAGTG 4 cut(s) 664, 1050, 2034, 2876
BtuMI TCGCGA 1 cut(s) 2646
BveI ACCTGC 1 cut(s) 1034
Cac8I GCNNGC 4 cut(s) 952, 1230, 2524, 3086
CciI TCATGA 3 cut(s) 355, 937, 1480
CfoI GCGC 3 cut(s) 1275, 2079, 2665
Cfr13I GGNCC 3 cut(s) 1632, 1676, 3183
Csp6I GTAC 7 cut(s) 329, 963, 1445, 1643, 1741, 1842, 3269
CspCI CAANNNNNGTGG 2 cut(s) 1556, 1591
CviQI GTAC 7 cut(s) 329, 963, 1445, 1643, 1741, 1842, 3269
DrdI GACNNNNNNGTC 1 cut(s) 2198
DseDI GACNNNNNNGTC 1 cut(s) 2198
Eam1104I CTCTTC 2 cut(s) 37, 495
EarI CTCTTC 2 cut(s) 37, 495
Eco130I CCWWGG 1 cut(s) 1973
Eco32I GATATC 1 cut(s) 1656
Eco47I GGWCC 2 cut(s) 1632, 1676
Eco47III AGCGCT 1 cut(s) 1274
Eco57I CTGAAG 5 cut(s) 27, 291, 1289, 1371, 2051
Eco88I CYCGRG 2 cut(s) 2095, 2495
Eco91I GGTNACC 1 cut(s) 2500
EcoO109I RGGNCCY 1 cut(s) 1676
EcoO65I GGTNACC 1 cut(s) 2500
EcoRII CCWGG 2 cut(s) 380, 2296
EcoRV GATATC 1 cut(s) 1656
EcoT14I CCWWGG 1 cut(s) 1973
EcoT22I ATGCAT 1 cut(s) 1470
ErhI CCWWGG 1 cut(s) 1973
Esp3I CGTCTC 1 cut(s) 800
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 3 cut(s) 1616, 2478, 2521
FauNDI CATATG 2 cut(s) 1193, 1311
FbaI TGATCA 1 cut(s) 2826
FblI GTMKAC 2 cut(s) 2878, 2913
GlaI GCGC 3 cut(s) 1274, 2078, 2664
GsaI CCCAGC 1 cut(s) 114
GsuI CTGGAG 3 cut(s) 410, 1654, 2249
HaeII RGCGCY 1 cut(s) 1276
HaeIII GGCC 2 cut(s) 539, 3184
HapII CCGG 5 cut(s) 74, 1503, 1587, 2505, 3079
HhaI GCGC 3 cut(s) 1275, 2079, 2665
Hin1I GRCGYC 1 cut(s) 2199
Hin6I GCGC 3 cut(s) 1273, 2077, 2663
HinP1I GCGC 3 cut(s) 1273, 2077, 2663
HincII GTYRAC 4 cut(s) 82, 421, 977, 2914
HindII GTYRAC 4 cut(s) 82, 421, 977, 2914
HindIII AAGCTT 2 cut(s) 2474, 2534
HpaII CCGG 5 cut(s) 74, 1503, 1587, 2505, 3079
HphI GGTGA 7 cut(s) 1763, 2051, 2201, 2257, 2449, 2881, 3028
Hpy166II GTNNAC 8 cut(s) 82, 421, 977, 1445, 1842, 2034, 2879, 2914
Hpy8I GTNNAC 8 cut(s) 82, 421, 977, 1445, 1842, 2034, 2879, 2914
Hpy99I CGWCG 4 cut(s) 1369, 2204, 2915, 3237
HpyCH4III ACNGT 9 cut(s) 659, 1289, 1365, 1607, 2443, 2864, 2911, 2999, 3273
HpyCH4IV ACGT 6 cut(s) 809, 934, 1441, 2199, 3154, 3235
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 1563, 1789, 2662, 2725
HpySE526I ACGT 6 cut(s) 809, 934, 1441, 2199, 3154, 3235
Hsp92I GRCGYC 1 cut(s) 2199
HspAI GCGC 3 cut(s) 1273, 2077, 2663
Kpn2I TCCGGA 1 cut(s) 1502
KpnI GGTACC 1 cut(s) 966
Ksp22I TGATCA 1 cut(s) 2826
LmnI GCTCC 4 cut(s) 20, 123, 1673, 2432
Lsp1109I GCAGC 9 cut(s) 102, 324, 327, 679, 1105, 1743, 2401, 2949, 2952
LweI GCATC 9 cut(s) 157, 1792, 1942, 1956, 2116, 2158, 2650, 2915, 3000
MaeII ACGT 6 cut(s) 809, 934, 1441, 2199, 3154, 3235
MaeIII GTNAC 9 cut(s) 748, 856, 925, 1579, 1824, 2119, 2189, 2437, 2500
MbiI CCGCTC 1 cut(s) 2435
MfeI CAATTG 2 cut(s) 597, 3064
MflI RGATCY 6 cut(s) 874, 1057, 1327, 1354, 1993, 2221
MhlI GDGCHC 3 cut(s) 1728, 2036, 2288
MlyI GAGTC 5 cut(s) 207, 512, 1101, 1529, 1570
MmeI TCCRAC 6 cut(s) 234, 1375, 1503, 2263, 2286, 2796
Mph1103I ATGCAT 1 cut(s) 1470
MroI TCCGGA 1 cut(s) 1502
MroXI GAANNNNTTC 1 cut(s) 2787
MslI CAYNNNNRTG 3 cut(s) 243, 942, 2154
MspA1I CMGCKG 1 cut(s) 110
MspCI CTTAAG 1 cut(s) 880
MspI CCGG 5 cut(s) 74, 1503, 1587, 2505, 3079
MspR9I CCNGG 4 cut(s) 382, 1587, 2298, 2506
MunI CAATTG 2 cut(s) 597, 3064
Mva1269I GAATGC 1 cut(s) 1045
MvaI CCWGG 2 cut(s) 382, 2298
MvnI CGCG 2 cut(s) 2079, 2646
MwoI GCNNNNNNNGC 5 cut(s) 217, 1563, 1789, 2662, 2725
NciI CCSGG 2 cut(s) 1587, 2506
NdeI CATATG 2 cut(s) 1193, 1311
NlaIV GGNNCC 3 cut(s) 378, 964, 1048
NmuCI GTSAC 7 cut(s) 748, 856, 925, 1579, 2119, 2189, 2437
NruI TCGCGA 1 cut(s) 2646
NsiI ATGCAT 1 cut(s) 1470
NspI RCATGY 5 cut(s) 787, 1031, 2120, 2697, 2728
NspV TTCGAA 2 cut(s) 3005, 3211
PagI TCATGA 3 cut(s) 355, 937, 1480
PaqCI CACCTGC 1 cut(s) 1034
PciI ACATGT 1 cut(s) 2693
PctI GAATGC 1 cut(s) 1045
PdmI GAANNNNTTC 1 cut(s) 2787
PflFI GACNNNGTC 2 cut(s) 659, 811
PleI GAGTC 5 cut(s) 206, 511, 1101, 1529, 1569
PpsI GAGTC 5 cut(s) 206, 511, 1101, 1529, 1569
PpuMI RGGWCCY 1 cut(s) 1676
PscI ACATGT 1 cut(s) 2693
PshAI GACNNNNGTC 1 cut(s) 369
PshBI ATTAAT 1 cut(s) 1919
Psp1406I AACGTT 1 cut(s) 3154
Psp5II RGGWCCY 1 cut(s) 1676
Psp6I CCWGG 2 cut(s) 380, 2296
PspEI GGTNACC 1 cut(s) 2500
PspFI CCCAGC 1 cut(s) 110
PspGI CCWGG 2 cut(s) 380, 2296
PspN4I GGNNCC 3 cut(s) 378, 964, 1048
PspPI GGNCC 3 cut(s) 1632, 1676, 3183
PspPPI RGGWCCY 1 cut(s) 1676
PstI CTGCAG 1 cut(s) 1057
PsuI RGATCY 6 cut(s) 874, 1057, 1327, 1354, 1993, 2221
PsyI GACNNNGTC 2 cut(s) 659, 811
RruI TCGCGA 1 cut(s) 2646
RsaI GTAC 7 cut(s) 330, 964, 1446, 1644, 1742, 1843, 3270
RsaNI GTAC 7 cut(s) 329, 963, 1445, 1643, 1741, 1842, 3269
RseI CAYNNNNRTG 3 cut(s) 243, 942, 2154
SalI GTCGAC 1 cut(s) 2912
Sau96I GGNCC 3 cut(s) 1632, 1676, 3183
SchI GAGTC 5 cut(s) 207, 512, 1101, 1529, 1570
ScrFI CCNGG 4 cut(s) 382, 1587, 2298, 2506
SduI GDGCHC 3 cut(s) 1728, 2036, 2288
SfaNI GCATC 9 cut(s) 157, 1792, 1942, 1956, 2116, 2158, 2650, 2915, 3000
SfcI CTRYAG 2 cut(s) 1053, 2995
SfuI TTCGAA 2 cut(s) 3005, 3211
SinI GGWCC 2 cut(s) 1632, 1676
SmiMI CAYNNNNRTG 3 cut(s) 243, 942, 2154
SmlI CTYRAG 4 cut(s) 332, 880, 1302, 2411
SmoI CTYRAG 4 cut(s) 332, 880, 1302, 2411
SsiI CCGC 9 cut(s) 108, 560, 746, 1630, 1930, 2079, 2435, 2717, 2989
SspI AATATT 3 cut(s) 1159, 1747, 2616
StyD4I CCNGG 4 cut(s) 380, 1585, 2296, 2504
StyI CCWWGG 1 cut(s) 1973
TaaI ACNGT 9 cut(s) 659, 1289, 1365, 1607, 2443, 2864, 2911, 2999, 3273
TaiI ACGT 6 cut(s) 812, 937, 1444, 2202, 3157, 3238
TatI WGTACW 2 cut(s) 1444, 1841
TauI GCSGC 2 cut(s) 1933, 2719
TscAI CASTG 4 cut(s) 664, 1057, 2041, 2876
TseFI GTSAC 7 cut(s) 748, 856, 925, 1579, 2119, 2189, 2437
TseI GCWGC 9 cut(s) 115, 312, 315, 667, 1118, 1756, 2389, 2962, 2965
Tsp45I GTSAC 7 cut(s) 748, 856, 925, 1579, 2119, 2189, 2437
TspGWI ACGGA 1 cut(s) 1050
TspRI CASTG 4 cut(s) 664, 1057, 2041, 2876
Tth111I GACNNNGTC 2 cut(s) 659, 811
Vha464I CTTAAG 1 cut(s) 880
VneI GTGCAC 1 cut(s) 2032
VpaK11BI GGWCC 2 cut(s) 1632, 1676
VspI ATTAAT 1 cut(s) 1919
XapI RAATTY 4 cut(s) 707, 824, 2629, 2900
XbaI TCTAGA 1 cut(s) 194
XceI RCATGY 5 cut(s) 787, 1031, 2120, 2697, 2728
XmiI GTMKAC 2 cut(s) 2878, 2913
XmnI GAANNNNTTC 1 cut(s) 2787
ZraI GACGTC 1 cut(s) 2200
Zsp2I ATGCAT 1 cut(s) 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.