Prupe.6G163400_v2.0.a1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0016489
Physical Location & Seq
Reverse (-)
15588789 .. 15592030
3242 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G163400.2

Sequence Viewer

Length: 1134 bp
ATGGCTGATACTGAGGACATTCAACCCCTTGTCTGTGACAATGGAACTGGAATGGTGAAGGCTGGATTTGCTGGTGACGACGCACCCAGGGCTGTGTTTCCTTCTATTGTTGGTCGACCACGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATGCCTATGTAGGTGATGAAGCCCAATCTAAGAGAGGTATCCTTACCTTGAAATATCCCATTGAACATGGTATAGTAAGCAACTGGGATGACATGGAGAAGATCTGGCATCACACTTTCTACAATGAGCTTCGTGTTGCTCCTGAAGAGCACCCAGTTCTTCTTACTGAGGCTCCTCTCAACCCTAAGGCTAACAGAGAAAAGATGACGCAGATCATGTTTGAGACCTTCAATGTGCCTGCCATGTATGTTGCCATCCAGGCCGTTCTCTCTCTGTATGCCAGTGGTCGTACAACTGGTATTGTGCTGGACTCTGGTGATGGTGTGAGTCACACTGTGCCAATCTATGAAGGTTATGCCCTACCTCATGCCATTCTTCGTCTGGACCTTGCTGGTCGTGACCTCACAGATGCCTTGATGAAGATTCTCACCGAAAGAGGGTACATGTTCACCACCACTGCTGAGCGGGAAATTGTCCGTGATATGAAGGAGAAGCTCGCATATGTTGCCCTGGACTATGAGCAAGAACTTGAGACTGCTAAGAGCAGCTCTTCAGTAGAGAAGAACTATGAGCTTCCCGATGGCCAAGTAATCACAATTGGAGCTGAGAGATTCCGGTGCCCAGAAGTTCTCTTCCAACCATCACTCATTGGAATGGAAGCTGCTGGAATTCATGAGACTACCTACAACTCTATCATGAAGTGTGATGTGGATATTAGGAAAGACCTATATGGAAACATTGTGCTCAGTGGTGGGTCAACTATGTTCCCCGGTATTGCAGACAGGATGAGCAAGGAGATTACTGCTCTTGCTCCAAGCAGCATGAAGATTAAGGTTGTGGCTCCACCAGAGAGAAAGTACAGTGTCTGGATTGGAGGGTCCATCCTTGCATCCCTCAGTACCTTCCAGCAGATGTGGATTTCCAAGGGTGAGTACGATGAGTCTGGTCCATCCATTGTCCACAGGAAGTGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Actin

Protein Analysis

378

Amino Acids

41.73

Weight (kDa)

5.31

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09810
fragaria_vesca FvH4_1g23490
malus_domestica MD01G1001600.v1.1
prunus_persica Prupe.6G163400_v2.0.a1 Prupe.6G163400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0118891
rosa_laevigata RLG00000018431
rosa_multiflora Rmu_sc0001653.1_g000037
rosa_roxburghii Rroxscaffold_2G00124610
rosa_rugosa Rorug02G0219400
rosa_samantha Rh2BG288200 Rh2DG283400 Rh2DG302700
rosa_wichuraiana Rw2G022010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 774
AccBSI CCGCTC 1 cut(s) 622
AccI GTMKAC 1 cut(s) 115
AciI CCGC 1 cut(s) 622
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 1 cut(s) 825
AcuI CTGAAG 2 cut(s) 321, 693
AdeI CACNNNGTG 1 cut(s) 493
AfaI GTAC 5 cut(s) 448, 599, 1016, 1057, 1091
AfiI CCNNNNNNNGG 1 cut(s) 594
AflIII ACRYGT 1 cut(s) 600
AgsI TTSAA 4 cut(s) 23, 208, 221, 388
AjnI CCWGG 3 cut(s) 86, 414, 666
AleI CACNNNNGTG 1 cut(s) 129
AluBI AGCT 6 cut(s) 286, 652, 705, 730, 761, 818
AluI AGCT 6 cut(s) 286, 652, 705, 730, 761, 818
Alw21I GWGCWC 2 cut(s) 309, 903
Alw26I GTCTC 3 cut(s) 374, 683, 827
AlwNI CAGNNNCTG 1 cut(s) 1023
AoxI GGCC 2 cut(s) 417, 739
ApeKI GCWGC 3 cut(s) 702, 818, 975
ApoI RAATTY 1 cut(s) 825
Asp700I GAANNNNTTC 1 cut(s) 1127
AspS9I GGNCC 3 cut(s) 541, 1035, 1103
AsuC2I CCSGG 1 cut(s) 927
AsuHPI GGTGA 7 cut(s) 67, 86, 182, 485, 577, 598, 1097
AvaII GGWCC 3 cut(s) 541, 1035, 1103
AxyI CCTNAGG 1 cut(s) 342
BaeGI GKGCMC 1 cut(s) 779
BalI TGGCCA 1 cut(s) 741
BanI GGYRCC 1 cut(s) 774
Bbv12I GWGCWC 2 cut(s) 309, 903
BbvI GCAGC 3 cut(s) 714, 805, 987
BccI CCATC 6 cut(s) 419, 470, 731, 805, 1046, 1114
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 3 cut(s) 88, 416, 668
BciVI GTATCC 1 cut(s) 206
BcnI CCSGG 1 cut(s) 927
BcoDI GTCTC 3 cut(s) 374, 683, 827
BfuI GTATCC 1 cut(s) 206
BglI GCCNNNNNGGC 1 cut(s) 416
BglII AGATCT 1 cut(s) 258
BisI GCNGC 3 cut(s) 703, 819, 976
BlpI GCTNAGC 1 cut(s) 618
BlsI GCNGC 3 cut(s) 704, 820, 977
Bme1390I CCNGG 4 cut(s) 88, 416, 668, 927
Bme18I GGWCC 3 cut(s) 541, 1035, 1103
BmgT120I GGNCC 3 cut(s) 541, 1035, 1103
BmiI GGNNCC 4 cut(s) 330, 776, 999, 1036
BmrFI CCNGG 4 cut(s) 88, 416, 668, 927
BmrI ACTGGG 2 cut(s) 250, 305
BmsI GCATC 4 cut(s) 148, 274, 556, 1055
BmuI ACTGGG 2 cut(s) 250, 305
Bpu1102I GCTNAGC 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 5 cut(s) 86, 87, 666, 925, 1080
BsaWI WCCGGW 1 cut(s) 771
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse1I ACTGG 6 cut(s) 52, 133, 245, 311, 438, 457
Bse21I CCTNAGG 1 cut(s) 342
BseBI CCWGG 3 cut(s) 88, 416, 668
BseDI CCNNGG 5 cut(s) 86, 87, 666, 925, 1080
BseGI GGATG 7 cut(s) 163, 250, 411, 948, 1038, 1046, 1106
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMII CTCAG 5 cut(s) 315, 609, 753, 916, 1066
BseNI ACTGG 6 cut(s) 52, 133, 245, 311, 438, 457
BseRI GAGGAG 1 cut(s) 321
BseSI GKGCMC 1 cut(s) 779
BseXI GCAGC 3 cut(s) 714, 805, 987
BshFI GGCC 2 cut(s) 419, 741
BshNI GGYRCC 1 cut(s) 774
BsiHKAI GWGCWC 2 cut(s) 309, 903
BsiSI CCGG 2 cut(s) 772, 927
BslI CCNNNNNNNGG 1 cut(s) 594
BsmAI GTCTC 3 cut(s) 374, 683, 827
BsnI GGCC 2 cut(s) 419, 741
Bso31I GGTCTC 1 cut(s) 374
Bsp1286I GDGCHC 3 cut(s) 309, 779, 903
Bsp143I GATC 2 cut(s) 258, 369
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 1 cut(s) 622
BspANI GGCC 2 cut(s) 419, 741
BspCNI CTCAG 6 cut(s) 4, 316, 610, 754, 915, 1065
BspHI TCATGA 2 cut(s) 829, 852
BspLI GGNNCC 4 cut(s) 330, 776, 999, 1036
BspQI GCTCTTC 2 cut(s) 297, 712
BspT107I GGYRCC 1 cut(s) 774
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 622
BsrI ACTGG 6 cut(s) 52, 133, 245, 311, 438, 457
BssECI CCNNGG 5 cut(s) 86, 87, 666, 925, 1080
BssMI GATC 2 cut(s) 258, 369
BssT1I CCWWGG 1 cut(s) 1080
Bst2UI CCWGG 3 cut(s) 88, 416, 668
Bst4CI ACNGT 2 cut(s) 493, 1019
Bst6I CTCTTC 3 cut(s) 297, 712, 794
BstAPI GCANNNNNTGC 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 396, 654
BstDEI CTNAG 9 cut(s) 12, 186, 324, 342, 618, 696, 762, 902, 1052
BstF5I GGATG 7 cut(s) 163, 250, 411, 948, 1038, 1046, 1106
BstKTI GATC 2 cut(s) 261, 372
BstMAI GTCTC 3 cut(s) 374, 683, 827
BstMBI GATC 2 cut(s) 258, 369
BstMWI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
BstNI CCWGG 3 cut(s) 88, 416, 668
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 4 cut(s) 86, 414, 666, 925
BstSLI GKGCMC 1 cut(s) 779
BstV1I GCAGC 3 cut(s) 714, 805, 987
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 342
BsuI GTATCC 1 cut(s) 206
BsuRI GGCC 2 cut(s) 419, 741
BtsCI GGATG 7 cut(s) 163, 250, 411, 948, 1038, 1046, 1106
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 6 cut(s) 126, 445, 489, 612, 910, 1024
Cac8I GCNNGC 2 cut(s) 396, 654
CaiI CAGNNNCTG 1 cut(s) 1023
CciI TCATGA 2 cut(s) 829, 852
Cfr13I GGNCC 3 cut(s) 541, 1035, 1103
CseI GACGC 2 cut(s) 89, 373
Csp6I GTAC 5 cut(s) 447, 598, 1015, 1056, 1090
CviAII CATG 9 cut(s) 224, 250, 373, 400, 524, 601, 830, 853, 979
CviQI GTAC 5 cut(s) 447, 598, 1015, 1056, 1090
DdeI CTNAG 9 cut(s) 12, 186, 324, 342, 618, 696, 762, 902, 1052
DpnI GATC 2 cut(s) 260, 371
DpnII GATC 2 cut(s) 258, 369
DraIII CACNNNGTG 1 cut(s) 493
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 3 cut(s) 297, 712, 794
EarI CTCTTC 3 cut(s) 297, 712, 794
Eco130I CCWWGG 1 cut(s) 1080
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 3 cut(s) 541, 1035, 1103
Eco57I CTGAAG 2 cut(s) 321, 693
Eco81I CCTNAGG 1 cut(s) 342
EcoRI GAATTC 1 cut(s) 825
EcoRII CCWGG 3 cut(s) 86, 414, 666
EcoT14I CCWWGG 1 cut(s) 1080
ErhI CCWWGG 1 cut(s) 1080
FaeI CATG 9 cut(s) 227, 253, 376, 403, 527, 604, 833, 856, 982
FatI CATG 9 cut(s) 223, 249, 372, 399, 523, 600, 829, 852, 978
FauI CCCGC 1 cut(s) 615
FauNDI CATATG 1 cut(s) 658
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 703, 819, 976
FokI GGATG 7 cut(s) 170, 257, 398, 955, 1025, 1033, 1093
Fsp4HI GCNGC 3 cut(s) 703, 819, 976
GluI GCNGC 3 cut(s) 703, 819, 976
HaeIII GGCC 2 cut(s) 419, 741
HapII CCGG 2 cut(s) 772, 927
HgaI GACGC 2 cut(s) 89, 373
Hin1II CATG 9 cut(s) 227, 253, 376, 403, 527, 604, 833, 856, 982
HincII GTYRAC 2 cut(s) 116, 915
HindII GTYRAC 2 cut(s) 116, 915
HinfI GANTC 5 cut(s) 467, 484, 580, 768, 1097
HpaII CCGG 2 cut(s) 772, 927
HphI GGTGA 7 cut(s) 67, 86, 182, 485, 577, 598, 1097
Hpy166II GTNNAC 4 cut(s) 116, 606, 915, 1117
Hpy188I TCNGA 1 cut(s) 1133
Hpy188III TCNNGA 7 cut(s) 299, 539, 554, 734, 830, 853, 1024
Hpy8I GTNNAC 4 cut(s) 116, 606, 915, 1117
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 6 cut(s) 52, 111, 394, 500, 637, 1069
HpyCH4III ACNGT 2 cut(s) 493, 1019
HpyCH4V TGCA 2 cut(s) 935, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
HpyF3I CTNAG 9 cut(s) 12, 186, 324, 342, 618, 696, 762, 902, 1052
Hsp92II CATG 9 cut(s) 227, 253, 376, 403, 527, 604, 833, 856, 982
Kzo9I GATC 2 cut(s) 258, 369
LguI GCTCTTC 2 cut(s) 297, 712
LmnI GCTCC 5 cut(s) 301, 334, 758, 973, 1003
Lsp1109I GCAGC 3 cut(s) 714, 805, 987
LweI GCATC 4 cut(s) 148, 274, 556, 1055
MaeIII GTNAC 4 cut(s) 35, 74, 485, 554
MalI GATC 2 cut(s) 260, 371
MbiI CCGCTC 1 cut(s) 622
MboI GATC 2 cut(s) 258, 369
MboII GAAGA 9 cut(s) 268, 308, 314, 524, 589, 699, 730, 781, 994
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 3 cut(s) 309, 779, 903
MlsI TGGCCA 1 cut(s) 741
MluCI AATT 3 cut(s) 627, 753, 825
MluNI TGGCCA 1 cut(s) 741
MlyI GAGTC 3 cut(s) 461, 493, 1106
MmeI TCCRAC 1 cut(s) 817
MnlI CCTC 9 cut(s) 7, 185, 319, 342, 531, 569, 587, 1025, 1061
Mox20I TGGCCA 1 cut(s) 741
MroXI GAANNNNTTC 1 cut(s) 1127
MscI TGGCCA 1 cut(s) 741
MseI TTAA 1 cut(s) 987
MslI CAYNNNNRTG 2 cut(s) 129, 809
Msp20I TGGCCA 1 cut(s) 741
MspI CCGG 2 cut(s) 772, 927
MspR9I CCNGG 4 cut(s) 88, 416, 668, 927
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 3 cut(s) 88, 416, 668
MwoI GCNNNNNNNGC 4 cut(s) 68, 89, 416, 662
NciI CCSGG 1 cut(s) 927
NdeI CATATG 1 cut(s) 658
NdeII GATC 2 cut(s) 258, 369
NlaIII CATG 9 cut(s) 227, 253, 376, 403, 527, 604, 833, 856, 982
NlaIV GGNNCC 4 cut(s) 330, 776, 999, 1036
NmuCI GTSAC 4 cut(s) 35, 74, 485, 554
NspI RCATGY 1 cut(s) 604
OliI CACNNNNGTG 1 cut(s) 129
PagI TCATGA 2 cut(s) 829, 852
PasI CCCWGGG 1 cut(s) 87
PciI ACATGT 1 cut(s) 600
PciSI GCTCTTC 2 cut(s) 297, 712
PdmI GAANNNNTTC 1 cut(s) 1127
PfeI GAWTC 2 cut(s) 580, 768
PkrI GCNGC 3 cut(s) 704, 820, 977
PleI GAGTC 3 cut(s) 461, 492, 1105
PpsI GAGTC 3 cut(s) 461, 492, 1105
PscI ACATGT 1 cut(s) 600
Psp6I CCWGG 3 cut(s) 86, 414, 666
PspGI CCWGG 3 cut(s) 86, 414, 666
PspN4I GGNNCC 4 cut(s) 330, 776, 999, 1036
PspPI GGNCC 3 cut(s) 541, 1035, 1103
PstNI CAGNNNCTG 1 cut(s) 1023
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 5 cut(s) 448, 599, 1016, 1057, 1091
RsaNI GTAC 5 cut(s) 447, 598, 1015, 1056, 1090
RseI CAYNNNNRTG 2 cut(s) 129, 809
SalI GTCGAC 1 cut(s) 114
SapI GCTCTTC 2 cut(s) 297, 712
SaqAI TTAA 1 cut(s) 987
SatI GCNGC 3 cut(s) 703, 819, 976
Sau3AI GATC 2 cut(s) 258, 369
Sau96I GGNCC 3 cut(s) 541, 1035, 1103
SchI GAGTC 3 cut(s) 461, 493, 1106
ScrFI CCNGG 4 cut(s) 88, 416, 668, 927
SduI GDGCHC 3 cut(s) 309, 779, 903
SfaNI GCATC 4 cut(s) 148, 274, 556, 1055
SinI GGWCC 3 cut(s) 541, 1035, 1103
SmiMI CAYNNNNRTG 2 cut(s) 129, 809
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
Sse9I AATT 3 cut(s) 627, 753, 825
SsiI CCGC 1 cut(s) 622
StyD4I CCNGG 4 cut(s) 86, 414, 666, 925
StyI CCWWGG 1 cut(s) 1080
TaaI ACNGT 2 cut(s) 493, 1019
TaqI TCGA 1 cut(s) 115
TasI AATT 3 cut(s) 627, 753, 825
TatI WGTACW 1 cut(s) 1014
TfiI GAWTC 2 cut(s) 580, 768
Tru1I TTAA 1 cut(s) 987
Tru9I TTAA 1 cut(s) 987
TscAI CASTG 6 cut(s) 133, 445, 496, 619, 910, 1024
TseFI GTSAC 4 cut(s) 35, 74, 485, 554
TseI GCWGC 3 cut(s) 702, 818, 975
Tsp45I GTSAC 4 cut(s) 35, 74, 485, 554
TspDTI ATGAA 7 cut(s) 189, 519, 590, 656, 818, 869, 995
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 6 cut(s) 133, 445, 496, 619, 910, 1024
VpaK11BI GGWCC 3 cut(s) 541, 1035, 1103
XapI RAATTY 1 cut(s) 825
XceI RCATGY 1 cut(s) 604
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.