Prupe.8G061700_v2.0.a1

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0013343
Physical Location & Seq
Forward (+)
8898774 .. 8905041
6268 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G061700.1

Sequence Viewer

Length: 828 bp
ATGGTGGTAGCATCAGCTTTCTCAGCGCCACTGAGCTTCTCATTCGCTTCTTGCCACAGAACCTCTTCTCTTCATTTGTTTTCTCTCAGAAACCCATCGTCAAAGTCCACAGTCTCTGCCTTGCAGAGTCCGTACGGCGATTCGTTAACAACTGGATTATCTAGTAGAACTCGTGGGTCACCTCTAAAGTTTGACGTGAAGGATTTTGGTGATACTAGTACAAGTTATGGTGCAATAGAAGCCAGAAAAGGGAATCCACCCATACTTCCAGCTGTGATGACCCCTGGTGGACCTTTGGACCTCTCATCTGTATTGTTCAGGAATCGCATAATCTTCATTGGGCAGCCAGTCAACTCATTGGTGGCTCAACGAGTCATATCCCAACTTGTTACTTTGGCTACTGTTGATGAGAATGCGGATATTCTGGTGTATTTGAACTGCCCTGGTGGGAGTACATACTCTGTGTTGGCAATTTATGACTGCATGTCTTGGATCAAACCTAAGGTGGGAACAGTGTGTTTTGGAGTAGCAGCAAGCCAAGGAGCACTTCTTCTTGCTGGTGGAGAAAAGGGAATGCGCTATGCGATGCCAAATGCGCGAATCATGATACACCAACCACAGAGTGGATGTGGGGGTCATGTGGAAGATGTGAGGCGCCAAGTGAATGAAGCAGTTCAAACTCGCCATAAAGTCGACAATATGTACGTTGCTTTTACTGGCCAGTCTCTAGAGAAAGTGCAACAGTACACTGAGAGGGATCGTTTCTTATCCGTTTCTGAGGCAATGGAATTTGGGCTTATTGATGGCGTGTTGGAAACAGAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04112 ko04212 map04112 map04212
Pfam Domains
Protein Families
CLP_protease

Protein Analysis

276

Amino Acids

29.7

Weight (kDa)

7.65

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11750 AT1G11750
fragaria_vesca FvH4_2g13930
malus_domestica MD05G1045200.v1.1 MD10G1052100.v1.1
prunus_persica Prupe.8G061700_v2.0.a1 Prupe.8G061700_v2.0.a1
pyrus_communis pycom10g03680
rosa_chinensis RchiOBHm_Chr6g0275681
rosa_laevigata RLG00000013447
rosa_multiflora Rmu_sc0016068.1_g000003 Rmu_sc0018708.1_g000009
rosa_roxburghii Rroxscaffold_7G00193030
rosa_rugosa Rorug06G0095600
rosa_samantha Rh6AG207500 Rh6CG213500 Rh6DG203200
rosa_wichuraiana Rw6G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 654
AccB7I CCANNNNNTGG 1 cut(s) 623
AccI GTMKAC 1 cut(s) 693
AccII CGCG 1 cut(s) 598
AciI CCGC 1 cut(s) 416
AclWI GGATC 2 cut(s) 500, 765
AcoI YGGCCR 1 cut(s) 718
AcsI RAATTY 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 655
AdeI CACNNNGTG 1 cut(s) 623
AfaI GTAC 5 cut(s) 134, 220, 454, 704, 746
AfiI CCNNNNNNNGG 3 cut(s) 249, 506, 623
AgsI TTSAA 2 cut(s) 436, 677
AhdI GACNNNNNGTC 1 cut(s) 484
AhlI ACTAGT 1 cut(s) 215
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 2 cut(s) 283, 442
AjuI GAANNNNNNNTTGG 2 cut(s) 531, 563
AluBI AGCT 3 cut(s) 17, 36, 272
AluI AGCT 3 cut(s) 17, 36, 272
Alw21I GWGCWC 1 cut(s) 547
Alw26I GTCTC 2 cut(s) 118, 729
AlwI GGATC 2 cut(s) 500, 765
AlwNI CAGNNNCTG 1 cut(s) 116
AoxI GGCC 1 cut(s) 718
ApeKI GCWGC 2 cut(s) 343, 530
ApoI RAATTY 1 cut(s) 788
Asp700I GAANNNNTTC 2 cut(s) 64, 672
AspLEI GCGC 4 cut(s) 28, 579, 598, 657
AspS9I GGNCC 2 cut(s) 290, 298
AsuHPI GGTGA 2 cut(s) 171, 221
AvaII GGWCC 2 cut(s) 290, 298
AxyI CCTNAGG 1 cut(s) 501
BalI TGGCCA 1 cut(s) 720
BanI GGYRCC 1 cut(s) 654
BauI CACGAG 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 547
BbvI GCAGC 2 cut(s) 355, 542
BccI CCATC 2 cut(s) 103, 797
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 2 cut(s) 285, 444
BcoDI GTCTC 2 cut(s) 118, 729
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 3 cut(s) 162, 216, 728
BfoI RGCGCY 2 cut(s) 29, 658
BisI GCNGC 2 cut(s) 344, 531
BlsI GCNGC 2 cut(s) 345, 532
Bme1390I CCNGG 2 cut(s) 285, 444
Bme18I GGWCC 2 cut(s) 290, 298
BmeRI GACNNNNNGTC 1 cut(s) 484
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 290, 298
BmiI GGNNCC 1 cut(s) 656
BmrFI CCNGG 2 cut(s) 285, 444
BmsI GCATC 2 cut(s) 20, 576
BsaHI GRCGYC 1 cut(s) 655
BsaJI CCNNGG 3 cut(s) 283, 442, 538
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 506, 623
Bse1I ACTGG 4 cut(s) 157, 347, 721, 721
Bse21I CCTNAGG 1 cut(s) 501
Bse3DI GCAATG 1 cut(s) 789
BseBI CCWGG 2 cut(s) 285, 444
BseDI CCNNGG 3 cut(s) 283, 442, 538
BseGI GGATG 1 cut(s) 632
BseLI CCNNNNNNNGG 3 cut(s) 249, 506, 623
BseMI GCAATG 1 cut(s) 789
BseMII CTCAG 5 cut(s) 23, 36, 100, 741, 768
BseNI ACTGG 4 cut(s) 157, 347, 721, 721
BseXI GCAGC 2 cut(s) 355, 542
Bsh1236I CGCG 1 cut(s) 598
BshFI GGCC 1 cut(s) 720
BshNI GGYRCC 1 cut(s) 654
BsiHKAI GWGCWC 1 cut(s) 547
BsiWI CGTACG 1 cut(s) 132
BslI CCNNNNNNNGG 3 cut(s) 249, 506, 623
BsmAI GTCTC 2 cut(s) 118, 729
BsmI GAATGC 2 cut(s) 418, 579
BsnI GGCC 1 cut(s) 720
Bsp1286I GDGCHC 1 cut(s) 547
Bsp143I GATC 2 cut(s) 492, 757
BspACI CCGC 1 cut(s) 416
BspANI GGCC 1 cut(s) 720
BspCNI CTCAG 5 cut(s) 24, 35, 99, 742, 769
BspFNI CGCG 1 cut(s) 598
BspHI TCATGA 1 cut(s) 603
BspLI GGNNCC 1 cut(s) 656
BspPI GGATC 2 cut(s) 500, 765
BspT107I GGYRCC 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 789
BsrI ACTGG 4 cut(s) 157, 347, 721, 721
BssECI CCNNGG 3 cut(s) 283, 442, 538
BssMI GATC 2 cut(s) 492, 757
BssNI GRCGYC 1 cut(s) 655
BssSI CACGAG 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 538
Bst2BI CACGAG 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 285, 444
Bst4CI ACNGT 4 cut(s) 112, 403, 514, 744
Bst6I CTCTTC 2 cut(s) 70, 75
BstACI GRCGYC 1 cut(s) 655
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 6 cut(s) 22, 32, 86, 501, 750, 777
BstEII GGTNACC 1 cut(s) 177
BstF5I GGATG 1 cut(s) 632
BstFNI CGCG 1 cut(s) 598
BstH2I RGCGCY 2 cut(s) 29, 658
BstHHI GCGC 4 cut(s) 28, 579, 598, 657
BstKTI GATC 2 cut(s) 495, 760
BstMAI GTCTC 2 cut(s) 118, 729
BstMBI GATC 2 cut(s) 492, 757
BstMWI GCNNNNNNNGC 3 cut(s) 23, 239, 595
BstNI CCWGG 2 cut(s) 285, 444
BstNSI RCATGY 1 cut(s) 487
BstPI GGTNACC 1 cut(s) 177
BstSCI CCNGG 2 cut(s) 283, 442
BstUI CGCG 1 cut(s) 598
BstV1I GCAGC 2 cut(s) 355, 542
Bsu36I CCTNAGG 1 cut(s) 501
BsuRI GGCC 1 cut(s) 720
BtgZI GCGATG 1 cut(s) 599
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 1 cut(s) 632
BtsIMutI CAGTG 3 cut(s) 29, 519, 747
Cac8I GCNNGC 1 cut(s) 535
CaiI CAGNNNCTG 1 cut(s) 116
CciI TCATGA 1 cut(s) 603
CfoI GCGC 4 cut(s) 28, 579, 598, 657
Cfr13I GGNCC 2 cut(s) 290, 298
Csp6I GTAC 5 cut(s) 133, 219, 453, 703, 745
CviAII CATG 3 cut(s) 484, 604, 638
CviQI GTAC 5 cut(s) 133, 219, 453, 703, 745
DdeI CTNAG 6 cut(s) 22, 32, 86, 501, 750, 777
DinI GGCGCC 1 cut(s) 656
DpnI GATC 2 cut(s) 494, 759
DpnII GATC 2 cut(s) 492, 757
DraIII CACNNNGTG 1 cut(s) 623
DriI GACNNNNNGTC 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 718
Eam1104I CTCTTC 2 cut(s) 70, 75
Eam1105I GACNNNNNGTC 1 cut(s) 484
EarI CTCTTC 2 cut(s) 70, 75
Eco130I CCWWGG 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 290, 298
Eco81I CCTNAGG 1 cut(s) 501
Eco91I GGTNACC 1 cut(s) 177
EcoO65I GGTNACC 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 283, 442
EcoT14I CCWWGG 1 cut(s) 538
EgeI GGCGCC 1 cut(s) 656
EheI GGCGCC 1 cut(s) 656
ErhI CCWWGG 1 cut(s) 538
FaeI CATG 3 cut(s) 487, 607, 641
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 3 cut(s) 483, 603, 637
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 2 cut(s) 344, 531
FokI GGATG 1 cut(s) 639
Fsp4HI GCNGC 2 cut(s) 344, 531
FspBI CTAG 3 cut(s) 162, 216, 728
GlaI GCGC 4 cut(s) 27, 578, 597, 656
GluI GCNGC 2 cut(s) 344, 531
HaeII RGCGCY 2 cut(s) 29, 658
HaeIII GGCC 1 cut(s) 720
HhaI GCGC 4 cut(s) 28, 579, 598, 657
Hin1I GRCGYC 1 cut(s) 655
Hin1II CATG 3 cut(s) 487, 607, 641
Hin6I GCGC 4 cut(s) 26, 577, 596, 655
HinP1I GCGC 4 cut(s) 26, 577, 596, 655
HincII GTYRAC 3 cut(s) 147, 352, 694
HindII GTYRAC 3 cut(s) 147, 352, 694
HinfI GANTC 6 cut(s) 127, 140, 253, 322, 372, 600
HpaI GTTAAC 1 cut(s) 147
HphI GGTGA 2 cut(s) 171, 221
Hpy166II GTNNAC 6 cut(s) 108, 147, 290, 352, 694, 747
Hpy188I TCNGA 2 cut(s) 89, 778
Hpy188III TCNNGA 3 cut(s) 319, 604, 728
Hpy8I GTNNAC 6 cut(s) 108, 147, 290, 352, 694, 747
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 4 cut(s) 112, 403, 514, 744
HpyCH4IV ACGT 2 cut(s) 195, 705
HpyCH4V TGCA 4 cut(s) 124, 233, 483, 739
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 239, 595
HpyF3I CTNAG 6 cut(s) 22, 32, 86, 501, 750, 777
HpySE526I ACGT 2 cut(s) 195, 705
Hsp92I GRCGYC 1 cut(s) 655
Hsp92II CATG 3 cut(s) 487, 607, 641
HspAI GCGC 4 cut(s) 26, 577, 596, 655
KasI GGCGCC 1 cut(s) 654
KspAI GTTAAC 1 cut(s) 147
Kzo9I GATC 2 cut(s) 492, 757
LmnI GCTCC 1 cut(s) 542
Lsp1109I GCAGC 2 cut(s) 355, 542
LweI GCATC 2 cut(s) 20, 576
MaeI CTAG 3 cut(s) 162, 216, 728
MaeII ACGT 2 cut(s) 195, 705
MaeIII GTNAC 2 cut(s) 177, 388
MalI GATC 2 cut(s) 494, 759
MboI GATC 2 cut(s) 492, 757
MboII GAAGA 5 cut(s) 57, 62, 325, 542, 656
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 1 cut(s) 720
MluCI AATT 2 cut(s) 471, 788
MluNI TGGCCA 1 cut(s) 720
Mly113I GGCGCC 1 cut(s) 655
MlyI GAGTC 2 cut(s) 136, 381
MmeI TCCRAC 1 cut(s) 792
MnlI CCTC 6 cut(s) 73, 192, 311, 645, 747, 772
Mox20I TGGCCA 1 cut(s) 720
MroXI GAANNNNTTC 2 cut(s) 64, 672
MscI TGGCCA 1 cut(s) 720
MseI TTAA 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 720
MspA1I CMGCKG 1 cut(s) 272
MspR9I CCNGG 2 cut(s) 285, 444
Mva1269I GAATGC 2 cut(s) 418, 579
MvaI CCWGG 2 cut(s) 285, 444
MvnI CGCG 1 cut(s) 598
MwoI GCNNNNNNNGC 3 cut(s) 23, 239, 595
NarI GGCGCC 1 cut(s) 655
NdeII GATC 2 cut(s) 492, 757
NlaIII CATG 3 cut(s) 487, 607, 641
NlaIV GGNNCC 1 cut(s) 656
NmuCI GTSAC 1 cut(s) 177
NspI RCATGY 1 cut(s) 487
PagI TCATGA 1 cut(s) 603
PctI GAATGC 2 cut(s) 418, 579
PdmI GAANNNNTTC 2 cut(s) 64, 672
PfeI GAWTC 4 cut(s) 140, 253, 322, 600
Pfl23II CGTACG 1 cut(s) 132
PflMI CCANNNNNTGG 1 cut(s) 623
PkrI GCNGC 2 cut(s) 345, 532
PleI GAGTC 2 cut(s) 135, 380
PluTI GGCGCC 1 cut(s) 658
PpsI GAGTC 2 cut(s) 135, 380
Psp6I CCWGG 2 cut(s) 283, 442
PspEI GGTNACC 1 cut(s) 177
PspGI CCWGG 2 cut(s) 283, 442
PspLI CGTACG 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 656
PspPI GGNCC 2 cut(s) 290, 298
PstNI CAGNNNCTG 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 5 cut(s) 134, 220, 454, 704, 746
RsaNI GTAC 5 cut(s) 133, 219, 453, 703, 745
SalI GTCGAC 1 cut(s) 692
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 2 cut(s) 344, 531
Sau3AI GATC 2 cut(s) 492, 757
Sau96I GGNCC 2 cut(s) 290, 298
SchI GAGTC 2 cut(s) 136, 381
ScrFI CCNGG 2 cut(s) 285, 444
SduI GDGCHC 1 cut(s) 547
SfaNI GCATC 2 cut(s) 20, 576
SfoI GGCGCC 1 cut(s) 656
SinI GGWCC 2 cut(s) 290, 298
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 2 cut(s) 471, 788
SsiI CCGC 1 cut(s) 416
SspDI GGCGCC 1 cut(s) 654
SspI AATATT 1 cut(s) 824
SspMI CTAG 3 cut(s) 162, 216, 728
StyD4I CCNGG 2 cut(s) 283, 442
StyI CCWWGG 1 cut(s) 538
TaaI ACNGT 4 cut(s) 112, 403, 514, 744
TaiI ACGT 2 cut(s) 198, 708
TaqI TCGA 1 cut(s) 693
TasI AATT 2 cut(s) 471, 788
TatI WGTACW 3 cut(s) 218, 452, 744
TfiI GAWTC 4 cut(s) 140, 253, 322, 600
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TscAI CASTG 3 cut(s) 36, 519, 754
TseFI GTSAC 1 cut(s) 177
TseI GCWGC 2 cut(s) 343, 530
Tsp45I GTSAC 1 cut(s) 177
TspDTI ATGAA 3 cut(s) 62, 325, 681
TspGWI ACGGA 2 cut(s) 120, 760
TspRI CASTG 3 cut(s) 36, 519, 754
Van91I CCANNNNNTGG 1 cut(s) 623
VpaK11BI GGWCC 2 cut(s) 290, 298
XapI RAATTY 1 cut(s) 788
XbaI TCTAGA 1 cut(s) 727
XceI RCATGY 1 cut(s) 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmiI GTMKAC 1 cut(s) 693
XmnI GAANNNNTTC 2 cut(s) 64, 672
XspI CTAG 3 cut(s) 162, 216, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.