pycom01g04230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0018514
Physical Location & Seq
Forward (+)
3173586 .. 3175767
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g04230.9

Sequence Viewer

Length: 1419 bp
ATGAATTCCAACACTCCAGATTGTGCCACGTGTCACAACGTAATATCGACCGTTGATCTCAGATTGAACGATCGAAAGGACCGTGGGAAGCCACGTGGCTTCCCAACGGTAACTTTTGAATCTGAAAAAGTGGTTAATTTTCTGAAATTGTGTCGGCGACGTGCCTGGCATCACATCCACTCGGGCTCTCGGGCTGACAATTCTTCACAAGCTCGGAGCTTGGGCCTCCACCTCCACTCGGGCTCCTTCACGCTGGAGCCGATCCATAGAGCCTCGGACCCTTTTCTCTGGTCTGTCCCCCGAGCAATCCACCACGGTGGAGTTGCTCTGAGTGCAGCCAAGTGGGCTGCTCTTTCTGTTCTTTCTTTGCAATCCAGCAGTAATTGCCAGGCTTCCATCCGACAGCTATACACGCGTGGGTGGAGTTGCTCTGAGTGCACAGTAATTGCCAGGCTTCCATCCGACAGCTATACACGCGTGTGCATGCATGTGCGCGGATCACAACCTTCTATCCCACATCTTCTTGCGTTCGATCCCCGGGTGTGTGTGTTTTTTTTTGTTTTTCTTTTATTGTTTTCTATTTTTCCTTTTAGTTTTCTTAAGTCTTCTCTTTCTTTTGGCTATTTTTTGTTATGTTATCTTCCACACCTTGAGGACAAAGTGTGCAATAAGTTTGGGGTTAAAATTTTTATTCTTTTTAAGTTAATATCTATAAAATATTTTAAATTCTTTTCTATTTATCATTTCACATCAACATGGTTTGTGAGGAGTGAGATTGAAACACTTACTTTTATAGTTATTGTTATGAATAAAGTAATAATTGTTCCACCCGAGTCTTTGCCTAGTAACCGAGCTAAGTTTTGGCATGGGGCAGGGTGGTTAGTTGGTTTCATAGCCTTTTCAGCTCAGGTTAATAAGTCCTTAGGGGTGTTCTACACCTATGCACGACCACTACTATTACTGAATATATATATATATATATATATATATATATATATATATATTACATATGAAAAAAAAATAGTTATATTTAAAGTTAGTATGTGTGAAATAATGAAGGACGAGAGAAAGTTTCTGGATTCTTTTCTAATTGATTCTAAATGGCTTAGACTCTTTTTAACATGTTCTATGGATGGCAACTTTGAAGGTGAAAATTTTTTTTTGTTTTCTAGTTTTATTTTGCTTGACAAGCAAAAAGCTAAGTTTGGGGGTATTTTGATGAGTCAATTTCATATTATATATTTTACTCTATTCTTAAATTGTATTTCTAATATAGGACTTTCGACTTCTTTTGGAGCAAAACATGGTCAAATGGTTGAATTTTGGAGTAATTCCAATTGGAGGACGTTCGTGAGTCACTTGGCTTTATCGTATCAAAATATCAGATTTTTCCACCAAGCGATGATTTTCTGGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

473

Amino Acids

54.6

Weight (kDa)

9.5

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 415, 477, 495
AciI CCGC 1 cut(s) 495
AclWI GGATC 3 cut(s) 256, 505, 527
AcsI RAATTY 5 cut(s) 4, 684, 725, 1153, 1319
AcvI CACGTG 2 cut(s) 30, 95
AfiI CCNNNNNNNGG 2 cut(s) 238, 1341
AflII CTTAAG 1 cut(s) 599
AflIII ACRYGT 4 cut(s) 29, 413, 475, 1121
AgsI TTSAA 5 cut(s) 67, 119, 779, 1145, 1319
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 3 cut(s) 164, 387, 449
AleI CACNNNNGTG 2 cut(s) 315, 478
AluBI AGCT 7 cut(s) 212, 219, 406, 468, 854, 905, 1199
AluI AGCT 7 cut(s) 212, 219, 406, 468, 854, 905, 1199
Alw21I GWGCWC 1 cut(s) 440
Alw44I GTGCAC 1 cut(s) 436
AlwI GGATC 3 cut(s) 256, 505, 527
Ama87I CYCGRG 6 cut(s) 181, 189, 238, 300, 537, 830
AoxI GGCC 1 cut(s) 223
ApaLI GTGCAC 1 cut(s) 436
ApeKI GCWGC 2 cut(s) 335, 347
ApoI RAATTY 5 cut(s) 4, 684, 725, 1153, 1319
AspLEI GCGC 1 cut(s) 495
AspS9I GGNCC 3 cut(s) 79, 223, 277
AsuC2I CCSGG 2 cut(s) 538, 539
AsuHPI GGTGA 1 cut(s) 1160
AvaI CYCGRG 6 cut(s) 181, 189, 238, 300, 537, 830
AvaII GGWCC 2 cut(s) 79, 277
AxyI CCTNAGG 1 cut(s) 922
BaeGI GKGCMC 1 cut(s) 440
BanII GRGCYC 2 cut(s) 188, 245
BbrPI CACGTG 2 cut(s) 30, 95
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 440
BbvI GCAGC 2 cut(s) 334, 347
BccI CCATC 3 cut(s) 404, 466, 1127
BciT130I CCWGG 3 cut(s) 166, 389, 451
BcnI CCSGG 2 cut(s) 538, 539
BfaI CTAG 2 cut(s) 843, 1170
BfrI CTTAAG 1 cut(s) 599
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 2 cut(s) 336, 348
BlsI GCNGC 2 cut(s) 337, 349
Bme1390I CCNGG 5 cut(s) 166, 389, 451, 538, 539
Bme18I GGWCC 2 cut(s) 79, 277
BmeT110I CYCGRG 6 cut(s) 181, 189, 238, 300, 537, 830
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 3 cut(s) 79, 223, 277
BmiI GGNNCC 3 cut(s) 244, 258, 279
BmrFI CCNGG 5 cut(s) 166, 389, 451, 538, 539
BmsI GCATC 1 cut(s) 178
BpiI GAAGAC 1 cut(s) 597
BpmI CTGGAG 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 671
BpuMI CCSGG 2 cut(s) 538, 539
BsaAI YACGTR 2 cut(s) 30, 95
BsaJI CCNNGG 5 cut(s) 82, 273, 313, 536, 537
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 1341
Bse21I CCTNAGG 1 cut(s) 922
BseBI CCWGG 3 cut(s) 166, 389, 451
BseDI CCNNGG 5 cut(s) 82, 273, 313, 536, 537
BseGI GGATG 4 cut(s) 174, 396, 458, 1138
BseLI CCNNNNNNNGG 2 cut(s) 238, 1341
BseMII CTCAG 4 cut(s) 73, 320, 423, 920
BseRI GAGGAG 1 cut(s) 781
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 2 cut(s) 334, 347
BsgI GTGCAG 1 cut(s) 354
Bsh1236I CGCG 3 cut(s) 415, 477, 495
Bsh1285I CGRYCG 2 cut(s) 51, 73
BshFI GGCC 1 cut(s) 225
BsiEI CGRYCG 2 cut(s) 51, 73
BsiHKAI GWGCWC 1 cut(s) 440
BsiHKCI CYCGRG 6 cut(s) 181, 189, 238, 300, 537, 830
BsiSI CCGG 1 cut(s) 538
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 2 cut(s) 238, 1341
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 1 cut(s) 225
BsoBI CYCGRG 6 cut(s) 181, 189, 238, 300, 537, 830
Bsp1286I GDGCHC 3 cut(s) 188, 245, 440
Bsp143I GATC 5 cut(s) 55, 70, 261, 497, 532
BspACI CCGC 1 cut(s) 495
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 4 cut(s) 72, 321, 424, 919
BspFNI CGCG 3 cut(s) 415, 477, 495
BspLI GGNNCC 3 cut(s) 244, 258, 279
BspPI GGATC 3 cut(s) 256, 505, 527
BspTI CTTAAG 1 cut(s) 599
BssECI CCNNGG 5 cut(s) 82, 273, 313, 536, 537
BssMI GATC 5 cut(s) 55, 70, 261, 497, 532
Bst2UI CCWGG 3 cut(s) 166, 389, 451
Bst4CI ACNGT 5 cut(s) 52, 83, 109, 317, 442
BstAFI CTTAAG 1 cut(s) 599
BstAPI GCANNNNNTGC 1 cut(s) 384
BstBAI YACGTR 2 cut(s) 30, 95
BstC8I GCNNGC 1 cut(s) 485
BstDEI CTNAG 8 cut(s) 59, 329, 432, 855, 906, 922, 1106, 1200
BstDSI CCRYGG 2 cut(s) 82, 313
BstF5I GGATG 4 cut(s) 174, 396, 458, 1138
BstFNI CGCG 3 cut(s) 415, 477, 495
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 5 cut(s) 58, 73, 264, 500, 535
BstMBI GATC 5 cut(s) 55, 70, 261, 497, 532
BstMCI CGRYCG 2 cut(s) 51, 73
BstMWI GCNNNNNNNGC 8 cut(s) 332, 344, 384, 412, 435, 474, 902, 1189
BstNI CCWGG 3 cut(s) 166, 389, 451
BstNSI RCATGY 3 cut(s) 487, 491, 1125
BstSCI CCNGG 5 cut(s) 164, 387, 449, 536, 537
BstSLI GKGCMC 1 cut(s) 440
BstUI CGCG 3 cut(s) 415, 477, 495
BstV1I GCAGC 2 cut(s) 334, 347
BstV2I GAAGAC 1 cut(s) 597
BstXI CCANNNNNNTGG 1 cut(s) 317
Bsu36I CCTNAGG 1 cut(s) 922
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 2 cut(s) 82, 313
BtgZI GCGATG 1 cut(s) 1415
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 4 cut(s) 174, 396, 458, 1138
Cac8I GCNNGC 1 cut(s) 485
CfoI GCGC 1 cut(s) 495
Cfr13I GGNCC 3 cut(s) 79, 223, 277
Cfr9I CCCGGG 1 cut(s) 537
CviAII CATG 6 cut(s) 484, 488, 756, 866, 1122, 1304
DdeI CTNAG 8 cut(s) 59, 329, 432, 855, 906, 922, 1106, 1200
DpnI GATC 5 cut(s) 57, 72, 263, 499, 534
DpnII GATC 5 cut(s) 55, 70, 261, 497, 532
DraI TTTAAA 2 cut(s) 724, 1033
Eco24I GRGCYC 2 cut(s) 188, 245
Eco47I GGWCC 2 cut(s) 79, 277
Eco72I CACGTG 2 cut(s) 30, 95
Eco81I CCTNAGG 1 cut(s) 922
Eco88I CYCGRG 6 cut(s) 181, 189, 238, 300, 537, 830
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 3 cut(s) 164, 387, 449
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 2 cut(s) 188, 245
FaeI CATG 6 cut(s) 487, 491, 759, 869, 1125, 1307
FaqI GGGAC 1 cut(s) 281
FatI CATG 6 cut(s) 483, 487, 755, 865, 1121, 1303
FauNDI CATATG 1 cut(s) 1009
Fnu4HI GCNGC 2 cut(s) 336, 348
FokI GGATG 4 cut(s) 161, 383, 445, 1145
FriOI GRGCYC 2 cut(s) 188, 245
Fsp4HI GCNGC 2 cut(s) 336, 348
FspBI CTAG 2 cut(s) 843, 1170
GlaI GCGC 1 cut(s) 494
GluI GCNGC 2 cut(s) 336, 348
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 538
HhaI GCGC 1 cut(s) 495
Hin1II CATG 6 cut(s) 487, 491, 759, 869, 1125, 1307
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HinfI GANTC 7 cut(s) 119, 833, 1079, 1094, 1110, 1222, 1354
HpaII CCGG 1 cut(s) 538
HphI GGTGA 1 cut(s) 1160
Hpy166II GTNNAC 1 cut(s) 438
Hpy188III TCNNGA 3 cut(s) 17, 1076, 1351
Hpy8I GTNNAC 1 cut(s) 438
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 4 cut(s) 256, 516, 1051, 1139
HpyCH4III ACNGT 5 cut(s) 52, 83, 109, 317, 442
HpyCH4IV ACGT 5 cut(s) 29, 39, 94, 160, 1346
HpyCH4V TGCA 7 cut(s) 335, 370, 438, 483, 487, 666, 944
HpyF10VI GCNNNNNNNGC 8 cut(s) 332, 344, 384, 412, 435, 474, 902, 1189
HpyF3I CTNAG 8 cut(s) 59, 329, 432, 855, 906, 922, 1106, 1200
HpySE526I ACGT 5 cut(s) 29, 39, 94, 160, 1346
Hsp92II CATG 6 cut(s) 487, 491, 759, 869, 1125, 1307
HspAI GCGC 1 cut(s) 493
Kzo9I GATC 5 cut(s) 55, 70, 261, 497, 532
LmnI GCTCC 4 cut(s) 216, 248, 256, 1295
Lsp1109I GCAGC 2 cut(s) 334, 347
LweI GCATC 1 cut(s) 178
MaeI CTAG 2 cut(s) 843, 1170
MaeII ACGT 5 cut(s) 29, 39, 94, 160, 1346
MaeIII GTNAC 4 cut(s) 32, 109, 845, 1355
MalI GATC 5 cut(s) 57, 72, 263, 499, 534
MboI GATC 5 cut(s) 55, 70, 261, 497, 532
MboII GAAGA 4 cut(s) 195, 512, 597, 632
MfeI CAATTG 1 cut(s) 1336
MhlI GDGCHC 3 cut(s) 188, 245, 440
MluI ACGCGT 2 cut(s) 413, 475
MlyI GAGTC 4 cut(s) 842, 1104, 1231, 1363
MmeI TCCRAC 3 cut(s) 33, 424, 486
MnlI CCTC 6 cut(s) 236, 242, 283, 646, 759, 1335
Mph1103I ATGCAT 1 cut(s) 489
MslI CAYNNNNRTG 4 cut(s) 315, 478, 488, 754
MspCI CTTAAG 1 cut(s) 599
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 5 cut(s) 166, 389, 451, 538, 539
MunI CAATTG 1 cut(s) 1336
MvaI CCWGG 3 cut(s) 166, 389, 451
MvnI CGCG 3 cut(s) 415, 477, 495
MwoI GCNNNNNNNGC 8 cut(s) 332, 344, 384, 412, 435, 474, 902, 1189
NciI CCSGG 2 cut(s) 538, 539
NdeI CATATG 1 cut(s) 1009
NdeII GATC 5 cut(s) 55, 70, 261, 497, 532
NlaIII CATG 6 cut(s) 487, 491, 759, 869, 1125, 1307
NlaIV GGNNCC 3 cut(s) 244, 258, 279
NmuCI GTSAC 2 cut(s) 32, 1355
NsiI ATGCAT 1 cut(s) 489
NspI RCATGY 3 cut(s) 487, 491, 1125
OliI CACNNNNGTG 2 cut(s) 315, 478
PaeI GCATGC 1 cut(s) 487
PciI ACATGT 1 cut(s) 1121
PcsI WCGNNNNNNNCGW 2 cut(s) 79, 257
PfeI GAWTC 3 cut(s) 119, 1079, 1094
PkrI GCNGC 2 cut(s) 337, 349
Ple19I CGATCG 1 cut(s) 73
PleI GAGTC 4 cut(s) 841, 1104, 1230, 1362
PmaCI CACGTG 2 cut(s) 30, 95
PmlI CACGTG 2 cut(s) 30, 95
PpsI GAGTC 4 cut(s) 841, 1104, 1230, 1362
Ppu21I YACGTR 2 cut(s) 30, 95
PscI ACATGT 1 cut(s) 1121
Psp6I CCWGG 3 cut(s) 164, 387, 449
PspCI CACGTG 2 cut(s) 30, 95
PspGI CCWGG 3 cut(s) 164, 387, 449
PspN4I GGNNCC 3 cut(s) 244, 258, 279
PspPI GGNCC 3 cut(s) 79, 223, 277
PsrI GAACNNNNNNTAC 2 cut(s) 807, 839
PvuI CGATCG 1 cut(s) 73
RseI CAYNNNNRTG 4 cut(s) 315, 478, 488, 754
SatI GCNGC 2 cut(s) 336, 348
Sau3AI GATC 5 cut(s) 55, 70, 261, 497, 532
Sau96I GGNCC 3 cut(s) 79, 223, 277
SchI GAGTC 4 cut(s) 842, 1104, 1231, 1363
ScrFI CCNGG 5 cut(s) 166, 389, 451, 538, 539
SduI GDGCHC 3 cut(s) 188, 245, 440
SfaNI GCATC 1 cut(s) 178
SinI GGWCC 2 cut(s) 79, 277
SmaI CCCGGG 1 cut(s) 539
SmiMI CAYNNNNRTG 4 cut(s) 315, 478, 488, 754
SmlI CTYRAG 2 cut(s) 599, 650
SmoI CTYRAG 2 cut(s) 599, 650
SphI GCATGC 1 cut(s) 487
SsiI CCGC 1 cut(s) 495
SspI AATATT 1 cut(s) 719
SspMI CTAG 2 cut(s) 843, 1170
StyD4I CCNGG 5 cut(s) 164, 387, 449, 536, 537
TaaI ACNGT 5 cut(s) 52, 83, 109, 317, 442
TaiI ACGT 5 cut(s) 32, 42, 97, 163, 1349
TaqI TCGA 4 cut(s) 47, 73, 531, 1283
TfiI GAWTC 3 cut(s) 119, 1079, 1094
TseFI GTSAC 2 cut(s) 32, 1355
TseI GCWGC 2 cut(s) 335, 347
Tsp45I GTSAC 2 cut(s) 32, 1355
TspDTI ATGAA 6 cut(s) 17, 821, 880, 1026, 1070, 1220
TspMI CCCGGG 1 cut(s) 537
Vha464I CTTAAG 1 cut(s) 599
VneI GTGCAC 1 cut(s) 436
VpaK11BI GGWCC 2 cut(s) 79, 277
XapI RAATTY 5 cut(s) 4, 684, 725, 1153, 1319
XceI RCATGY 3 cut(s) 487, 491, 1125
XmaI CCCGGG 1 cut(s) 537
XspI CTAG 2 cut(s) 843, 1170
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.