pycom17g17910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0018514
Physical Location & Seq
Forward (+)
15452493 .. 15453422
930 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g17910.1

Sequence Viewer

Length: 552 bp
ATGACAACTGATGAGCGGATTTTATATCATATATTTAACCCTATTCTTAGACTTTCGACTTCCTCTGGAGGAAAACATGGTCAAATGGACAAATTTTGGAGTAATTCTAGTTGGAAGACAATCATGAGTCACTTAAAAAGAGCAATGCACAGTGCTGGAATAATGACGTTTTGGGCTTCTGGAGACCTCATATGGGTTCGTGGCCTTCTGGAGATATGTTCAAAACGTCCTGATCGATCCGGATTCAAAATAAACTATTATGGGCCGGATTTGGAGGAGATTTGTGTAGGACTTTATACTTGGTTCCTAGGGTTTGTGCGGCTAGCTTTGAGGGAGATATTTAAGGTTTTAGTCAACCCTAGTTTGGGACCACGGTTCTTAGAGATTTTCTACTTTAAGGTGTTTTCATTCCTTTTATTCTTAATAATATTTTCTATGATTTCAATTATGAATATGCTGCATGCACACTTTGAATTATTAATTACCAAAAGTAACTTGATGCAATTTTGGTCAGAAGGTTCCCTGAAATTGACGCTGGCTTCTTGTGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

21.21

Weight (kDa)

9.38

Isoelectric Point (pI)

29.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 16
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 2 cut(s) 16, 319
AclWI GGATC 1 cut(s) 231
AcsI RAATTY 1 cut(s) 92
AfiI CCNNNNNNNGG 3 cut(s) 193, 364, 365
AgsI TTSAA 4 cut(s) 222, 247, 444, 473
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 231
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 2 cut(s) 202, 263
ApeKI GCWGC 1 cut(s) 457
ApoI RAATTY 1 cut(s) 92
AseI ATTAAT 1 cut(s) 479
AspA2I CCTAGG 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 263, 368
AsuNHI GCTAGC 1 cut(s) 322
AvaII GGWCC 1 cut(s) 368
AvrII CCTAGG 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 444
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 4 cut(s) 108, 308, 323, 360
BisI GCNGC 2 cut(s) 320, 458
BlnI CCTAGG 1 cut(s) 307
BlsI GCNGC 2 cut(s) 321, 459
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 2 cut(s) 263, 368
BmiI GGNNCC 3 cut(s) 305, 369, 520
BmsI GCATC 1 cut(s) 489
BmtI GCTAGC 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 122
BpmI CTGGAG 3 cut(s) 87, 201, 230
Bsa29I ATCGAT 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 307, 371
BsaWI WCCGGW 1 cut(s) 239
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 364, 365
Bse3DI GCAATG 1 cut(s) 150
BseAI TCCGGA 1 cut(s) 239
BseCI ATCGAT 1 cut(s) 235
BseDI CCNNGG 2 cut(s) 307, 371
BseLI CCNNNNNNNGG 3 cut(s) 193, 364, 365
BseMI GCAATG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 1 cut(s) 444
BshFI GGCC 2 cut(s) 204, 265
BshVI ATCGAT 1 cut(s) 235
BsiSI CCGG 2 cut(s) 240, 266
BslFI GGGAC 1 cut(s) 381
BslI CCNNNNNNNGG 3 cut(s) 193, 364, 365
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 1 cut(s) 381
BsnI GGCC 2 cut(s) 204, 265
Bso31I GGTCTC 1 cut(s) 177
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 2 cut(s) 232, 236
BspACI CCGC 2 cut(s) 16, 319
BspANI GGCC 2 cut(s) 204, 265
BspDI ATCGAT 1 cut(s) 235
BspEI TCCGGA 1 cut(s) 239
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 3 cut(s) 305, 369, 520
BspOI GCTAGC 1 cut(s) 326
BspPI GGATC 1 cut(s) 231
BspTNI GGTCTC 1 cut(s) 177
BsrBI CCGCTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 150
BssECI CCNNGG 2 cut(s) 307, 371
BssMI GATC 2 cut(s) 232, 236
BssT1I CCWWGG 1 cut(s) 307
Bst4CI ACNGT 2 cut(s) 152, 375
BstC8I GCNNGC 3 cut(s) 324, 462, 537
BstDEI CTNAG 2 cut(s) 47, 379
BstDSI CCRYGG 1 cut(s) 371
BstKTI GATC 2 cut(s) 235, 239
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 232, 236
BstNSI RCATGY 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 444
BstV2I GAAGAC 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 235
BsuRI GGCC 2 cut(s) 204, 265
BsuTUI ATCGAT 1 cut(s) 235
BtgI CCRYGG 1 cut(s) 371
BtsIMutI CAGTG 1 cut(s) 157
Cac8I GCNNGC 3 cut(s) 324, 462, 537
CciI TCATGA 1 cut(s) 123
Cfr13I GGNCC 2 cut(s) 263, 368
ClaI ATCGAT 1 cut(s) 235
CseI GACGC 1 cut(s) 541
CviAII CATG 3 cut(s) 77, 124, 461
CviJI RGCY 6 cut(s) 176, 204, 265, 322, 326, 539
CviKI_1 RGCY 6 cut(s) 176, 204, 265, 322, 326, 539
DdeI CTNAG 2 cut(s) 47, 379
DpnI GATC 2 cut(s) 234, 238
DpnII GATC 2 cut(s) 232, 236
Eco130I CCWWGG 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 307
ErhI CCWWGG 1 cut(s) 307
FaeI CATG 3 cut(s) 80, 127, 464
FaqI GGGAC 1 cut(s) 381
FatI CATG 3 cut(s) 76, 123, 460
FauNDI CATATG 1 cut(s) 191
Fnu4HI GCNGC 2 cut(s) 320, 458
Fsp4HI GCNGC 2 cut(s) 320, 458
FspBI CTAG 4 cut(s) 108, 308, 323, 360
GluI GCNGC 2 cut(s) 320, 458
GsuI CTGGAG 3 cut(s) 87, 201, 230
HaeIII GGCC 2 cut(s) 204, 265
HapII CCGG 2 cut(s) 240, 266
HgaI GACGC 1 cut(s) 541
Hin1II CATG 3 cut(s) 80, 127, 464
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 2 cut(s) 127, 243
HpaII CCGG 2 cut(s) 240, 266
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 1 cut(s) 514
Hpy188III TCNNGA 6 cut(s) 66, 124, 180, 209, 230, 240
Hpy8I GTNNAC 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 215, 509
HpyCH4III ACNGT 2 cut(s) 152, 375
HpyCH4IV ACGT 2 cut(s) 167, 226
HpyCH4V TGCA 4 cut(s) 148, 460, 464, 502
HpyF3I CTNAG 2 cut(s) 47, 379
HpySE526I ACGT 2 cut(s) 167, 226
Hsp92II CATG 3 cut(s) 80, 127, 464
Kpn2I TCCGGA 1 cut(s) 239
Kzo9I GATC 2 cut(s) 232, 236
LpnPI CCDG 9 cut(s) 51, 141, 165, 194, 243, 253, 279, 521, 536
Lsp1109I GCAGC 1 cut(s) 444
LweI GCATC 1 cut(s) 489
MaeI CTAG 4 cut(s) 108, 308, 323, 360
MaeII ACGT 2 cut(s) 167, 226
MaeIII GTNAC 2 cut(s) 128, 491
MalI GATC 2 cut(s) 234, 238
MbiI CCGCTC 1 cut(s) 16
MboI GATC 2 cut(s) 232, 236
MboII GAAGA 1 cut(s) 127
MluCI AATT 7 cut(s) 92, 103, 444, 473, 480, 503, 527
MlyI GAGTC 1 cut(s) 136
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 5 cut(s) 62, 73, 197, 268, 324
MroI TCCGGA 1 cut(s) 239
MseI TTAA 7 cut(s) 36, 134, 342, 396, 422, 479, 550
MspI CCGG 2 cut(s) 240, 266
NdeI CATATG 1 cut(s) 191
NdeII GATC 2 cut(s) 232, 236
NheI GCTAGC 1 cut(s) 322
NlaIII CATG 3 cut(s) 80, 127, 464
NlaIV GGNNCC 3 cut(s) 305, 369, 520
NmuCI GTSAC 1 cut(s) 128
NspI RCATGY 1 cut(s) 464
PaeI GCATGC 1 cut(s) 464
PagI TCATGA 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PfeI GAWTC 1 cut(s) 243
PkrI GCNGC 2 cut(s) 321, 459
PleI GAGTC 1 cut(s) 135
PpsI GAGTC 1 cut(s) 135
PshBI ATTAAT 1 cut(s) 479
PspN4I GGNNCC 3 cut(s) 305, 369, 520
PspPI GGNCC 2 cut(s) 263, 368
SaqAI TTAA 7 cut(s) 36, 134, 342, 396, 422, 479, 550
SatI GCNGC 2 cut(s) 320, 458
Sau3AI GATC 2 cut(s) 232, 236
Sau96I GGNCC 2 cut(s) 263, 368
SchI GAGTC 1 cut(s) 136
SetI ASST 7 cut(s) 170, 189, 229, 328, 348, 402, 520
SfaNI GCATC 1 cut(s) 489
SinI GGWCC 1 cut(s) 368
SphI GCATGC 1 cut(s) 464
Sse9I AATT 7 cut(s) 92, 103, 444, 473, 480, 503, 527
SsiI CCGC 2 cut(s) 16, 319
SspI AATATT 1 cut(s) 429
SspMI CTAG 4 cut(s) 108, 308, 323, 360
StyI CCWWGG 1 cut(s) 307
TaaI ACNGT 2 cut(s) 152, 375
TaiI ACGT 2 cut(s) 170, 229
TaqI TCGA 2 cut(s) 56, 235
TasI AATT 7 cut(s) 92, 103, 444, 473, 480, 503, 527
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 1 cut(s) 243
Tru1I TTAA 7 cut(s) 36, 134, 342, 396, 422, 479, 550
Tru9I TTAA 7 cut(s) 36, 134, 342, 396, 422, 479, 550
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 2 cut(s) 396, 464
TspRI CASTG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 368
VspI ATTAAT 1 cut(s) 479
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 464
XmaJI CCTAGG 1 cut(s) 307
XspI CTAG 4 cut(s) 108, 308, 323, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.