pycom01g11260

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0017758
Physical Location & Seq
Forward (+)
12239393 .. 12241487
2095 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1272 bp
ATGTGTTACAGCTATGTGAAAAGGCGCGATTTTACCAGGTATATTTACCAATACCATACAATGTTGGCTCATAATTCATTCAGTTTAAGATTACTTACTACAAGGTCTCTTGGCATCTTTGAGGTTTGTGGCATAATTCATACTAGCGGACATCAGTATCTTGCTGCTCTGGATTGCTATATGAAAGGTGTAGAAGAACCCATTCATGCCTTCTCCTTTATCAACAAAACATTATTACAGCTGACTGACAAGGAATGCGCTGCTTTTCAATCAGAAGTAATTTCTCGAATTCCAGAGCTTTTTTACTCAAACAGAGAGAGCACATTCTTCTTGGTTATCGATCATTTTACTATTGAAGAAGGTTCTCATATACTCTCTAAACTTCGTTCCCATCCAAAAAGTCTGTTCCTTTACTTAAAAACAGTGATTGAGGTCCACTTATCTGGCACACTCGACTTTTCAAGTTTAAGAAGAGGTGATCATGTGAGGGATAAGGATCAGTCGAAAGCAGTTGAGGCTTATCTGGAAAGGATCTCTGATTTCCCAAAGCTTTTGCATAGTAATCCTGTTAATGTGACTGATGATATGATTGAACTGTATCTGGAGCTGTTATGTCAGTACGAGCGCAATTCAGTTCTCAAATTCCTAGAGACGTTCGATAGCTATCGCGTGGAACACTGTTTACGCCTGTGCCAGAAATATGGGATAACAGATGCTGCATCTTTCTTGTTGGAAAAGGTTGGTGATGTCAGCAGTGCTCTTTTACTTATACTTTCGACCATCGGTGACAAATTTATCAAGCTTGATACTGCTGTGGCAAGTCTGGTGTCAAGTAATTCCGCTCGTAGTGAGCATTTCAGCAATGCTTTAAAATTGGAGGAGGTGAACGACACGACATTAACAGTATATTGCATGCCTGTATTGGGCTGTGCCAAAGGAACACCCATCTTTTCCACGAGCACGATTATCTGGCTATTATACAGTCATAGGTTTCAGCTATGTAAAGGAGATGTTAAAACGATGGTAGCCGAGTCATTGGAGTCAGAAGAGGATGAGATGGCTTTTATAATCAAGTGGAGAATCTCAAATCTCCATAAAGGTTTCCATATCTTGAGAAAACTGTTCTCTCAGTTCATCAAGGAGATTGTTGAGGGCATGATAGGATGTGTTCGTCTTCCAACTATCATGTCCAAACTCGGTTCTGATAATGGCAGCCAGGAGTTTGGTGATTTTAAATTCACAATATTGGGGATGCTTAGCACGTATAGCTAG

Protein Analysis

424

Amino Acids

48.35

Weight (kDa)

6.35

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_Vps41 PF23556 191 - 263 1.9e-08 Vps41 TPR-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017758)

Species Orthologous Gene IDs
pyrus_communis pycom01g11260
rosa_chinensis RchiOBHm_Chr1g0361181
rosa_roxburghii Rroxscaffold_4G00295290
rosa_rugosa Rorug01G0287300
rosa_samantha Rh1AG298100 Rh1AG298200 Rh1BG262100 Rh1CG279500 Rh1DG292200 Rh1DG292300
rosa_wichuraiana Rw0G002910 Rw1G026360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1067
AccBSI CCGCTC 1 cut(s) 842
AccII CGCG 2 cut(s) 27, 669
AciI CCGC 2 cut(s) 147, 840
AclWI GGATC 2 cut(s) 504, 539
AcsI RAATTY 4 cut(s) 288, 641, 791, 1235
AfaI GTAC 1 cut(s) 620
AfiI CCNNNNNNNGG 1 cut(s) 923
AgsI TTSAA 4 cut(s) 269, 356, 462, 593
AjnI CCWGG 2 cut(s) 35, 1215
AluBI AGCT 9 cut(s) 12, 241, 298, 550, 607, 663, 802, 997, 1269
AluI AGCT 9 cut(s) 12, 241, 298, 550, 607, 663, 802, 997, 1269
Alw21I GWGCWC 3 cut(s) 323, 760, 962
Alw26I GTCTC 2 cut(s) 111, 644
AlwI GGATC 2 cut(s) 504, 539
AlwNI CAGNNNCTG 1 cut(s) 716
ApeKI GCWGC 4 cut(s) 164, 260, 716, 1212
ApoI RAATTY 4 cut(s) 288, 641, 791, 1235
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 3 cut(s) 27, 260, 627
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 5 cut(s) 488, 755, 797, 895, 1238
AvaII GGWCC 1 cut(s) 433
BaeI ACNNNNGTAYC 4 cut(s) 140, 173, 798, 831
BauI CACGAG 1 cut(s) 955
BbsI GAAGAC 1 cut(s) 1166
Bbv12I GWGCWC 3 cut(s) 323, 760, 962
BbvI GCAGC 4 cut(s) 151, 247, 703, 1224
BccI CCATC 5 cut(s) 399, 788, 953, 1015, 1051
BciT130I CCWGG 2 cut(s) 37, 1217
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 111, 644
BfaI CTAG 3 cut(s) 144, 647, 1270
BisI GCNGC 4 cut(s) 165, 261, 717, 1213
BlpI GCTNAGC 1 cut(s) 1256
BlsI GCNGC 4 cut(s) 166, 262, 718, 1214
Bme1390I CCNGG 2 cut(s) 37, 1217
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 433
BmrFI CCNGG 2 cut(s) 37, 1217
BmsI GCATC 4 cut(s) 123, 703, 728, 1242
BpiI GAAGAC 1 cut(s) 1166
BpmI CTGGAG 1 cut(s) 623
Bpu1102I GCTNAGC 1 cut(s) 1256
BpuEI CTTGAG 1 cut(s) 1132
Bsa29I ATCGAT 1 cut(s) 339
BsaAI YACGTR 1 cut(s) 1263
BsaBI GATNNNNATC 2 cut(s) 495, 663
BsaI GGTCTC 1 cut(s) 111
Bsc4I CCNNNNNNNGG 1 cut(s) 923
Bse3DI GCAATG 1 cut(s) 868
Bse8I GATNNNNATC 2 cut(s) 495, 663
BseBI CCWGG 2 cut(s) 37, 1217
BseCI ATCGAT 1 cut(s) 339
BseGI GGATG 4 cut(s) 391, 1057, 1169, 1257
BseJI GATNNNNATC 2 cut(s) 495, 663
BseLI CCNNNNNNNGG 1 cut(s) 923
BseMI GCAATG 1 cut(s) 868
BseMII CTCAG 1 cut(s) 1142
BseRI GAGGAG 1 cut(s) 893
BseXI GCAGC 4 cut(s) 151, 247, 703, 1224
Bsh1236I CGCG 2 cut(s) 27, 669
BshVI ATCGAT 1 cut(s) 339
BsiHKAI GWGCWC 3 cut(s) 323, 760, 962
BslI CCNNNNNNNGG 1 cut(s) 923
BsmAI GTCTC 2 cut(s) 111, 644
BsmBI CGTCTC 1 cut(s) 644
BsmI GAATGC 1 cut(s) 260
Bso31I GGTCTC 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 323, 760, 962
Bsp143I GATC 4 cut(s) 340, 478, 496, 531
Bsp1720I GCTNAGC 1 cut(s) 1256
BspACI CCGC 2 cut(s) 147, 840
BspCNI CTCAG 1 cut(s) 1141
BspDI ATCGAT 1 cut(s) 339
BspFNI CGCG 2 cut(s) 27, 669
BspPI GGATC 2 cut(s) 504, 539
BspTNI GGTCTC 1 cut(s) 111
BsrBI CCGCTC 1 cut(s) 842
BsrDI GCAATG 1 cut(s) 868
BssMI GATC 4 cut(s) 340, 478, 496, 531
BssSI CACGAG 1 cut(s) 955
Bst2BI CACGAG 1 cut(s) 955
Bst2UI CCWGG 2 cut(s) 37, 1217
Bst4CI ACNGT 6 cut(s) 424, 597, 680, 904, 983, 1122
Bst6I CTCTTC 2 cut(s) 466, 1041
BstBAI YACGTR 1 cut(s) 1263
BstC8I GCNNGC 1 cut(s) 914
BstDEI CTNAG 2 cut(s) 1128, 1256
BstF5I GGATG 4 cut(s) 391, 1057, 1169, 1257
BstFNI CGCG 2 cut(s) 27, 669
BstHHI GCGC 3 cut(s) 27, 260, 627
BstKTI GATC 4 cut(s) 343, 481, 499, 534
BstMAI GTCTC 2 cut(s) 111, 644
BstMBI GATC 4 cut(s) 340, 478, 496, 531
BstMWI GCNNNNNNNGC 2 cut(s) 515, 1266
BstNI CCWGG 2 cut(s) 37, 1217
BstNSI RCATGY 1 cut(s) 916
BstSCI CCNGG 2 cut(s) 35, 1215
BstUI CGCG 2 cut(s) 27, 669
BstV1I GCAGC 4 cut(s) 151, 247, 703, 1224
BstV2I GAAGAC 1 cut(s) 1166
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 3 cut(s) 443, 701, 1223
BstYI RGATCY 1 cut(s) 531
Bsu15I ATCGAT 1 cut(s) 339
BsuTUI ATCGAT 1 cut(s) 339
BtsCI GGATG 4 cut(s) 391, 1057, 1169, 1257
BtsI GCAGTG 1 cut(s) 760
BtsIMutI CAGTG 3 cut(s) 429, 676, 760
Cac8I GCNNGC 1 cut(s) 914
CaiI CAGNNNCTG 1 cut(s) 716
CfoI GCGC 3 cut(s) 27, 260, 627
Cfr13I GGNCC 1 cut(s) 433
ClaI ATCGAT 1 cut(s) 339
CsiI ACCWGGT 1 cut(s) 35
Csp6I GTAC 1 cut(s) 619
CviAII CATG 5 cut(s) 206, 482, 913, 1156, 1186
CviQI GTAC 1 cut(s) 619
DdeI CTNAG 2 cut(s) 1128, 1256
DpnI GATC 4 cut(s) 342, 480, 498, 533
DpnII GATC 4 cut(s) 340, 478, 496, 531
DraI TTTAAA 2 cut(s) 870, 1234
Eam1104I CTCTTC 2 cut(s) 466, 1041
EarI CTCTTC 2 cut(s) 466, 1041
Eco31I GGTCTC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 433
EcoRI GAATTC 1 cut(s) 288
EcoRII CCWGG 2 cut(s) 35, 1215
Esp3I CGTCTC 1 cut(s) 644
FaeI CATG 5 cut(s) 209, 485, 916, 1159, 1189
FatI CATG 5 cut(s) 205, 481, 912, 1155, 1185
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 4 cut(s) 165, 261, 717, 1213
FokI GGATG 4 cut(s) 378, 1064, 1176, 1264
Fsp4HI GCNGC 4 cut(s) 165, 261, 717, 1213
FspBI CTAG 3 cut(s) 144, 647, 1270
GlaI GCGC 3 cut(s) 26, 259, 626
GluI GCNGC 4 cut(s) 165, 261, 717, 1213
GsuI CTGGAG 1 cut(s) 623
HhaI GCGC 3 cut(s) 27, 260, 627
Hin1II CATG 5 cut(s) 209, 485, 916, 1159, 1189
Hin6I GCGC 3 cut(s) 25, 258, 625
HinP1I GCGC 3 cut(s) 25, 258, 625
HindIII AAGCTT 2 cut(s) 548, 800
HinfI GANTC 3 cut(s) 1031, 1040, 1080
HphI GGTGA 5 cut(s) 488, 755, 797, 895, 1238
Hpy166II GTNNAC 3 cut(s) 436, 683, 886
Hpy188I TCNGA 4 cut(s) 274, 538, 1045, 1204
Hpy188III TCNNGA 6 cut(s) 170, 285, 293, 524, 602, 1111
Hpy8I GTNNAC 3 cut(s) 436, 683, 886
HpyAV CCTTC 2 cut(s) 220, 353
HpyCH4III ACNGT 6 cut(s) 424, 597, 680, 904, 983, 1122
HpyCH4IV ACGT 2 cut(s) 653, 1262
HpyCH4V TGCA 3 cut(s) 556, 719, 912
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 1266
HpyF3I CTNAG 2 cut(s) 1128, 1256
HpySE526I ACGT 2 cut(s) 653, 1262
Hsp92II CATG 5 cut(s) 209, 485, 916, 1159, 1189
HspAI GCGC 3 cut(s) 25, 258, 625
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 4 cut(s) 340, 478, 496, 531
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 4 cut(s) 151, 247, 703, 1224
LweI GCATC 4 cut(s) 123, 703, 728, 1242
MabI ACCWGGT 1 cut(s) 35
MaeI CTAG 3 cut(s) 144, 647, 1270
MaeII ACGT 2 cut(s) 653, 1262
MaeIII GTNAC 3 cut(s) 5, 574, 785
MalI GATC 4 cut(s) 342, 480, 498, 533
MbiI CCGCTC 1 cut(s) 842
MboI GATC 4 cut(s) 340, 478, 496, 531
MboII GAAGA 6 cut(s) 206, 319, 368, 483, 1058, 1166
MflI RGATCY 1 cut(s) 531
MhlI GDGCHC 3 cut(s) 323, 760, 962
MlyI GAGTC 2 cut(s) 1040, 1049
MmeI TCCRAC 2 cut(s) 711, 1202
MnlI CCTC 9 cut(s) 115, 424, 467, 480, 508, 871, 874, 1042, 1144
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 8 cut(s) 86, 416, 467, 570, 869, 899, 1014, 1233
MspA1I CMGCKG 1 cut(s) 241
MspR9I CCNGG 2 cut(s) 37, 1217
Mva1269I GAATGC 1 cut(s) 260
MvaI CCWGG 2 cut(s) 37, 1217
MvnI CGCG 2 cut(s) 27, 669
MwoI GCNNNNNNNGC 2 cut(s) 515, 1266
NdeII GATC 4 cut(s) 340, 478, 496, 531
NlaIII CATG 5 cut(s) 209, 485, 916, 1159, 1189
NmeAIII GCCGAG 1 cut(s) 1054
NmuCI GTSAC 2 cut(s) 574, 785
NspI RCATGY 1 cut(s) 916
PaeI GCATGC 1 cut(s) 916
PctI GAATGC 1 cut(s) 260
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 1080
PkrI GCNGC 4 cut(s) 166, 262, 718, 1214
PleI GAGTC 2 cut(s) 1039, 1048
PpsI GAGTC 2 cut(s) 1039, 1048
Ppu21I YACGTR 1 cut(s) 1263
PsiI TTATAA 1 cut(s) 1067
Psp6I CCWGG 2 cut(s) 35, 1215
PspGI CCWGG 2 cut(s) 35, 1215
PspPI GGNCC 1 cut(s) 433
PsrI GAACNNNNNNTAC 2 cut(s) 666, 698
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 1 cut(s) 531
PvuII CAGCTG 1 cut(s) 241
RsaI GTAC 1 cut(s) 620
RsaNI GTAC 1 cut(s) 619
SaqAI TTAA 8 cut(s) 86, 416, 467, 570, 869, 899, 1014, 1233
SatI GCNGC 4 cut(s) 165, 261, 717, 1213
Sau3AI GATC 4 cut(s) 340, 478, 496, 531
Sau96I GGNCC 1 cut(s) 433
SchI GAGTC 2 cut(s) 1040, 1049
ScrFI CCNGG 2 cut(s) 37, 1217
SduI GDGCHC 3 cut(s) 323, 760, 962
SexAI ACCWGGT 1 cut(s) 35
SfaNI GCATC 4 cut(s) 123, 703, 728, 1242
SinI GGWCC 1 cut(s) 433
SmlI CTYRAG 1 cut(s) 1111
SmoI CTYRAG 1 cut(s) 1111
SphI GCATGC 1 cut(s) 916
SsiI CCGC 2 cut(s) 147, 840
SspI AATATT 1 cut(s) 1245
SspMI CTAG 3 cut(s) 144, 647, 1270
StyD4I CCNGG 2 cut(s) 35, 1215
TaaI ACNGT 6 cut(s) 424, 597, 680, 904, 983, 1122
TaiI ACGT 2 cut(s) 656, 1265
TaqI TCGA 6 cut(s) 286, 339, 453, 503, 657, 776
TfiI GAWTC 1 cut(s) 1080
Tru1I TTAA 8 cut(s) 86, 416, 467, 570, 869, 899, 1014, 1233
Tru9I TTAA 8 cut(s) 86, 416, 467, 570, 869, 899, 1014, 1233
TscAI CASTG 3 cut(s) 429, 683, 760
TseFI GTSAC 2 cut(s) 574, 785
TseI GCWGC 4 cut(s) 164, 260, 716, 1212
Tsp45I GTSAC 2 cut(s) 574, 785
TspDTI ATGAA 5 cut(s) 66, 128, 194, 197, 1123
TspRI CASTG 3 cut(s) 429, 683, 760
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 4 cut(s) 288, 641, 791, 1235
XceI RCATGY 1 cut(s) 916
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 3 cut(s) 144, 647, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.