Rorug01G0287300

Vacuolar protein sorting-associated protein 8 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017758
Physical Location & Seq
Forward (+)
40194275 .. 40197349
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0287300.1

Sequence Viewer

Length: 1128 bp
ATGGCGTCTTTGACGAGCTTTCTGAAGAAGAGATCCACTCTTTCTGTGTGCAATGAAGCCCTGAGAATGAAGAGACTCTTCTCCACTGCACAACCCATCGCTGCAGATTCTTCTTCTTTTGTTGAAAGGATCAGAAATCTCCCTAAAGCTCTTCCTGGAACCCACATCAAAACTGAAGTCTCCCAACTTATTGGTAGAACACCCCTAGTATACCTGAACAAAGTCACTGAAGGCTGTGGAGCTTATATTGCTGTCAAGCAAGAGATGTTTCAGCCCACCGCCAGCATCAAAGACAGACCAGCACTTGCTATGTTCAATGATGCTGAAAAGAAAGGCTTGATAACTCCTGGAGTGACAACTCTGATAGAGCCAACATCAGGAAACATGGGAATCAGCATGGCTTTTATGGCCGCGATGAAAGGGTACAAGATGGTTTTGACCATGCCCTCTTACACGAGCTTGGAGAGAAGAGTGTGTATGAGAGCATTCGGAGCTGAGTTGATTCTCACTGACCCTACAAAAGGGATGGGAGGAACTGTCAAGAAGGCTTATGACCTTTTGGAATCCACTCCAAATGCCCTTATGCTCCAACAATTCTCAAACCCTGCCAATACTCAGGTACATTTTGAAACTACAGGACCTGAGATATGGGAGGATACTAATGGAAAAGTTGACATCTTTGTAATGGGCATTGGTAGCGGAGGCACCGTCTCTGGGGTTGGACAGTATCTCAGATCTCAAAACCCTGATTGTAAGATATATGGAGTGGAGCCTGCTGAAAGTAATATATTAAATGGTGGTAAACCAGGTCCTCATTCAATTACTGGAAATGGGGTTGGATTCAAACCCGATATATTGGACATGGACATAATGGAAAGAGTTCTTGAGGTTAGAAGTGAAGATGCAGTAAATATGGCAAGGCAATTGGCACTGAAAGAAGGACTTTTGGTAGGGATATCATCTGGAGCCAACACAGTGGCAGCCCTTGAGCTCGCAAAAAAGCCAGAAAACAAAGGCAAACTTATAGTGACAATTCATGCAAGTTTTGGGGAGCGATACTTGTCGTCTGTCCTATTTGAAGAGCTAAGGAAGGAAGCTGAAAACATGCAGCCAGTCTCAGTTGATTAA

Protein Analysis

375

Amino Acids

40.59

Weight (kDa)

8.21

Isoelectric Point (pI)

34.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 60 - 346 4e-73 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017758)

Species Orthologous Gene IDs
pyrus_communis pycom01g11260
rosa_chinensis RchiOBHm_Chr1g0361181
rosa_roxburghii Rroxscaffold_4G00295290
rosa_rugosa Rorug01G0287300
rosa_samantha Rh1AG298100 Rh1AG298200 Rh1BG262100 Rh1CG279500 Rh1DG292200 Rh1DG292300
rosa_wichuraiana Rw0G002910 Rw1G026360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 704
AccI GTMKAC 1 cut(s) 210
AccII CGCG 1 cut(s) 413
AciI CCGC 3 cut(s) 279, 411, 699
AclWI GGATC 2 cut(s) 27, 137
AcoI YGGCCR 1 cut(s) 408
AcuI CTGAAG 3 cut(s) 44, 195, 249
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 425, 621
AfiI CCNNNNNNNGG 3 cut(s) 377, 521, 714
AgsI TTSAA 6 cut(s) 125, 316, 629, 819, 844, 1079
AjnI CCWGG 3 cut(s) 154, 346, 805
AluBI AGCT 8 cut(s) 18, 149, 242, 459, 494, 991, 1084, 1097
AluI AGCT 8 cut(s) 18, 149, 242, 459, 494, 991, 1084, 1097
Alw21I GWGCWC 1 cut(s) 993
Alw26I GTCTC 4 cut(s) 67, 184, 715, 1120
AlwI GGATC 2 cut(s) 27, 137
AlwNI CAGNNNCTG 1 cut(s) 641
AoxI GGCC 1 cut(s) 408
ApeKI GCWGC 3 cut(s) 101, 980, 1108
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
Asp700I GAANNNNTTC 1 cut(s) 879
AspS9I GGNCC 2 cut(s) 638, 809
AvaII GGWCC 2 cut(s) 638, 809
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 1 cut(s) 993
BauI CACGAG 1 cut(s) 454
Bbv12I GWGCWC 1 cut(s) 993
BbvI GCAGC 3 cut(s) 88, 992, 1120
BccI CCATC 3 cut(s) 104, 424, 520
BciT130I CCWGG 3 cut(s) 156, 348, 807
BciVI GTATCC 1 cut(s) 649
BcoDI GTCTC 4 cut(s) 67, 184, 715, 1120
BfaI CTAG 1 cut(s) 206
BfmI CTRYAG 2 cut(s) 102, 633
BfuI GTATCC 1 cut(s) 649
BglII AGATCT 1 cut(s) 734
BisI GCNGC 4 cut(s) 102, 411, 981, 1109
BlsI GCNGC 4 cut(s) 103, 412, 982, 1110
Bme1390I CCNGG 3 cut(s) 156, 348, 807
Bme18I GGWCC 2 cut(s) 638, 809
BmgT120I GGNCC 2 cut(s) 638, 809
BmiI GGNNCC 4 cut(s) 160, 706, 771, 967
BmrFI CCNGG 3 cut(s) 156, 348, 807
BmsI GCATC 3 cut(s) 294, 310, 892
BplI GAGNNNNNCTC 2 cut(s) 22, 54
BpmI CTGGAG 2 cut(s) 369, 984
Bpu10I CCTNAGC 1 cut(s) 1085
BpuEI CTTGAG 2 cut(s) 905, 1007
BsaHI GRCGYC 1 cut(s) 5
BsaXI ACNNNNNCTCC 4 cut(s) 522, 552, 693, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 377, 521, 714
Bse1I ACTGG 2 cut(s) 829, 1112
Bse3DI GCAATG 1 cut(s) 58
BseBI CCWGG 3 cut(s) 156, 348, 807
BseGI GGATG 1 cut(s) 531
BseLI CCNNNNNNNGG 3 cut(s) 377, 521, 714
BseMI GCAATG 1 cut(s) 58
BseMII CTCAG 5 cut(s) 53, 486, 629, 633, 745
BseNI ACTGG 2 cut(s) 829, 1112
BseXI GCAGC 3 cut(s) 88, 992, 1120
BsgI GTGCAG 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 413
BshFI GGCC 1 cut(s) 410
BshNI GGYRCC 1 cut(s) 704
BsiHKAI GWGCWC 1 cut(s) 993
BslI CCNNNNNNNGG 3 cut(s) 377, 521, 714
BsmAI GTCTC 4 cut(s) 67, 184, 715, 1120
BsmBI CGTCTC 1 cut(s) 715
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 993
Bsp143I GATC 3 cut(s) 32, 129, 734
BspACI CCGC 3 cut(s) 279, 411, 699
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 5 cut(s) 54, 487, 628, 634, 744
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 4 cut(s) 160, 706, 771, 967
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 2 cut(s) 27, 137
BspQI GCTCTTC 2 cut(s) 156, 1074
BspT107I GGYRCC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 58
BsrI ACTGG 2 cut(s) 829, 1112
BssMI GATC 3 cut(s) 32, 129, 734
BssNAI GTATAC 1 cut(s) 211
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 454
Bst1107I GTATAC 1 cut(s) 211
Bst2BI CACGAG 1 cut(s) 454
Bst2UI CCWGG 3 cut(s) 156, 348, 807
Bst4CI ACNGT 4 cut(s) 538, 709, 726, 976
Bst6I CTCTTC 6 cut(s) 23, 65, 83, 156, 463, 1074
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 3 cut(s) 283, 774, 993
BstDEI CTNAG 7 cut(s) 62, 495, 615, 642, 731, 1085, 1117
BstENI CCTNNNNNAGG 1 cut(s) 519
BstF5I GGATG 1 cut(s) 531
BstFNI CGCG 1 cut(s) 413
BstKTI GATC 3 cut(s) 35, 132, 737
BstMAI GTCTC 4 cut(s) 67, 184, 715, 1120
BstMBI GATC 3 cut(s) 32, 129, 734
BstMWI GCNNNNNNNGC 4 cut(s) 248, 407, 491, 696
BstNI CCWGG 3 cut(s) 156, 348, 807
BstNSI RCATGY 1 cut(s) 1108
BstSCI CCNGG 3 cut(s) 154, 346, 805
BstSFI CTRYAG 2 cut(s) 102, 633
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 3 cut(s) 88, 992, 1120
BstX2I RGATCY 2 cut(s) 32, 734
BstXI CCANNNNNNTGG 2 cut(s) 191, 976
BstYI RGATCY 2 cut(s) 32, 734
BstZ17I GTATAC 1 cut(s) 211
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 1 cut(s) 410
BtgZI GCGATG 2 cut(s) 82, 428
BtsCI GGATG 1 cut(s) 531
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 5 cut(s) 84, 225, 507, 929, 981
Cac8I GCNNGC 3 cut(s) 283, 774, 993
CaiI CAGNNNCTG 1 cut(s) 641
Cfr13I GGNCC 2 cut(s) 638, 809
CsiI ACCWGGT 1 cut(s) 805
Csp6I GTAC 2 cut(s) 424, 620
CviAII CATG 6 cut(s) 385, 397, 442, 862, 1037, 1105
CviQI GTAC 2 cut(s) 424, 620
DdeI CTNAG 7 cut(s) 62, 495, 615, 642, 731, 1085, 1117
DpnI GATC 3 cut(s) 34, 131, 736
DpnII GATC 3 cut(s) 32, 129, 734
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 6 cut(s) 23, 65, 83, 156, 463, 1074
EarI CTCTTC 6 cut(s) 23, 65, 83, 156, 463, 1074
Ecl136II GAGCTC 1 cut(s) 991
Eco24I GRGCYC 1 cut(s) 993
Eco32I GATATC 1 cut(s) 957
Eco47I GGWCC 2 cut(s) 638, 809
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 3 cut(s) 44, 195, 249
EcoICRI GAGCTC 1 cut(s) 991
EcoNI CCTNNNNNAGG 1 cut(s) 519
EcoO109I RGGNCCY 2 cut(s) 638, 809
EcoRII CCWGG 3 cut(s) 154, 346, 805
EcoRV GATATC 1 cut(s) 957
EcoT38I GRGCYC 1 cut(s) 993
Esp3I CGTCTC 1 cut(s) 715
FaeI CATG 6 cut(s) 388, 400, 445, 865, 1040, 1108
FalI AAGNNNNNCTT 8 cut(s) 62, 94, 320, 352, 927, 959, 1005, 1037
FatI CATG 6 cut(s) 384, 396, 441, 861, 1036, 1104
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 4 cut(s) 102, 411, 981, 1109
FokI GGATG 1 cut(s) 538
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 4 cut(s) 102, 411, 981, 1109
FspBI CTAG 1 cut(s) 206
GluI GCNGC 4 cut(s) 102, 411, 981, 1109
GsuI CTGGAG 2 cut(s) 369, 984
HaeIII GGCC 1 cut(s) 410
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 388, 400, 445, 865, 1040, 1108
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 6 cut(s) 75, 107, 390, 502, 563, 840
Hpy166II GTNNAC 3 cut(s) 211, 673, 803
Hpy188I TCNGA 5 cut(s) 24, 134, 363, 491, 734
Hpy188III TCNNGA 4 cut(s) 378, 541, 884, 963
Hpy8I GTNNAC 3 cut(s) 211, 673, 803
HpyAV CCTTC 4 cut(s) 224, 538, 932, 1084
HpyCH4III ACNGT 4 cut(s) 538, 709, 726, 976
HpyCH4V TGCA 6 cut(s) 51, 89, 104, 905, 1040, 1108
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 407, 491, 696
HpyF3I CTNAG 7 cut(s) 62, 495, 615, 642, 731, 1085, 1117
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 388, 400, 445, 865, 1040, 1108
Kzo9I GATC 3 cut(s) 32, 129, 734
LguI GCTCTTC 2 cut(s) 156, 1074
LmnI GCTCC 6 cut(s) 239, 491, 591, 769, 965, 1051
Lsp1109I GCAGC 3 cut(s) 88, 992, 1120
LweI GCATC 3 cut(s) 294, 310, 892
MabI ACCWGGT 1 cut(s) 805
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 3 cut(s) 223, 352, 1027
MalI GATC 3 cut(s) 34, 131, 736
MboI GATC 3 cut(s) 32, 129, 734
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 2 cut(s) 32, 734
MhlI GDGCHC 1 cut(s) 993
MluCI AATT 4 cut(s) 593, 819, 923, 1032
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 3 cut(s) 613, 700, 817
MnlI CCTC 6 cut(s) 457, 524, 646, 695, 822, 880
MroXI GAANNNNTTC 1 cut(s) 879
MseI TTAA 2 cut(s) 791, 1126
MspR9I CCNGG 3 cut(s) 156, 348, 807
MunI CAATTG 1 cut(s) 923
Mva1269I GAATGC 1 cut(s) 485
MvaI CCWGG 3 cut(s) 156, 348, 807
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 4 cut(s) 248, 407, 491, 696
NdeII GATC 3 cut(s) 32, 129, 734
NlaIII CATG 6 cut(s) 388, 400, 445, 865, 1040, 1108
NlaIV GGNNCC 4 cut(s) 160, 706, 771, 967
NmuCI GTSAC 3 cut(s) 223, 352, 1027
NspI RCATGY 1 cut(s) 1108
PciSI GCTCTTC 2 cut(s) 156, 1074
PctI GAATGC 1 cut(s) 485
PdmI GAANNNNTTC 1 cut(s) 879
PfeI GAWTC 5 cut(s) 107, 390, 502, 563, 840
PfoI TCCNGGA 2 cut(s) 154, 346
PkrI GCNGC 4 cut(s) 103, 412, 982, 1110
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
PpuMI RGGWCCY 2 cut(s) 638, 809
Psp124BI GAGCTC 1 cut(s) 993
Psp5II RGGWCCY 2 cut(s) 638, 809
Psp6I CCWGG 3 cut(s) 154, 346, 805
PspGI CCWGG 3 cut(s) 154, 346, 805
PspN4I GGNNCC 4 cut(s) 160, 706, 771, 967
PspPI GGNCC 2 cut(s) 638, 809
PspPPI RGGWCCY 2 cut(s) 638, 809
PstI CTGCAG 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 32, 734
RsaI GTAC 2 cut(s) 425, 621
RsaNI GTAC 2 cut(s) 424, 620
SacI GAGCTC 1 cut(s) 993
SapI GCTCTTC 2 cut(s) 156, 1074
SaqAI TTAA 2 cut(s) 791, 1126
SatI GCNGC 4 cut(s) 102, 411, 981, 1109
Sau3AI GATC 3 cut(s) 32, 129, 734
Sau96I GGNCC 2 cut(s) 638, 809
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 3 cut(s) 156, 348, 807
SduI GDGCHC 1 cut(s) 993
SexAI ACCWGGT 1 cut(s) 805
SfaNI GCATC 3 cut(s) 294, 310, 892
SfcI CTRYAG 2 cut(s) 102, 633
SinI GGWCC 2 cut(s) 638, 809
SmlI CTYRAG 2 cut(s) 884, 986
SmoI CTYRAG 2 cut(s) 884, 986
Sse9I AATT 4 cut(s) 593, 819, 923, 1032
SsiI CCGC 3 cut(s) 279, 411, 699
SspMI CTAG 1 cut(s) 206
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 3 cut(s) 154, 346, 805
TaaI ACNGT 4 cut(s) 538, 709, 726, 976
TasI AATT 4 cut(s) 593, 819, 923, 1032
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 5 cut(s) 107, 390, 502, 563, 840
Tru1I TTAA 2 cut(s) 791, 1126
Tru9I TTAA 2 cut(s) 791, 1126
TscAI CASTG 5 cut(s) 91, 232, 514, 936, 981
TseFI GTSAC 3 cut(s) 223, 352, 1027
TseI GCWGC 3 cut(s) 101, 980, 1108
Tsp45I GTSAC 3 cut(s) 223, 352, 1027
TspDTI ATGAA 4 cut(s) 69, 83, 431, 1025
TspRI CASTG 5 cut(s) 91, 232, 514, 936, 981
VpaK11BI GGWCC 2 cut(s) 638, 809
XagI CCTNNNNNAGG 1 cut(s) 519
XceI RCATGY 1 cut(s) 1108
XmiI GTMKAC 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 879
XspI CTAG 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.