pycom01g14680

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014133
Physical Location & Seq
Forward (+)
14757252 .. 14760084
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
ATGATGCTGTATTTGTTTCATTACTGCCTTTTATGTGCAAAGTGTATTAAGCTTGTGTCAGTACTGACATATGCCTTAATATATATATATACACACACATACTCAAAGCTCATCAACTCTCGCTCCATAACGAACCGTTCTGCCCCATTACCTTTCAGTTTCATCTTCCACACTCTCTACACCCTCGGCTCTCTAATGGCTCTCGAGGTCGCTGCCAAGGCCGCTGTCGGCGCCCCTGATCTCCTCGGCGACTGCCCTTTCTGCCAAAGGGTTACTCTAACCTTGGAGGAGAAGAAAGTACCCTACAAGCTGCACCTCATCAATCTCAGCGACAAACCCAAATGGTTTACGGAAGTGAATCCAGAGGGGAAGGTGCCTGTGGTGAAGTTTGATGACAAATGGGTGCCTGATTCCGATGTGATTGTTGGGATCATTGAGGAAAAATACCCTGAGCCTTCTCTCAAAACCCCTCCTGAATTTGCTTCTGTGGGATCAAAGATATTGGGATCATTTGTGACATTTCTGAAGAGTAAGGATCCCGGTGATGGATCAGAACAGGCTTTGCTTACCGAACTGAAGGCATTAGATGAGCATCTTAAGGCACATGGTCCGTACATTGCTGGGGAGAAGGTCACTGCTGCGGATCTAAGCTTGGCACCAAAACTGTACCATCTCAAGGTGGCTCTCAGCCATTTCAAGAAGTGGACGGTTCCAGCAGATTTGGCCCATTACCATAAGTACACCGAGCTGCTTTTCTCAAGGGAATCTTTCGTGAAGACCGCTCCTGCTGACGAGAAATATGTGATTGCAGGATGGGAGCCGAAGGTGAATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005777 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0006082 GO:0006464 GO:0006518 GO:0006575 GO:0006732 GO:0006749 GO:0006766 GO:0006767 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009753 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010193 GO:0010646 GO:0010647 GO:0010731 GO:0015036 GO:0015037 GO:0015038 GO:0016020 GO:0016209 GO:0016491 GO:0016667 GO:0016672 GO:0016740 GO:0016765 GO:0016999 GO:0017001 GO:0017144 GO:0019538 GO:0019752 GO:0019852 GO:0023051 GO:0023056 GO:0033218 GO:0033355 GO:0033554 GO:0034599 GO:0034614 GO:0034641 GO:0035690 GO:0036211 GO:0042221 GO:0042277 GO:0042493 GO:0042542 GO:0042579 GO:0042737 GO:0042743 GO:0042744 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043412 GO:0043436 GO:0043603 GO:0043900 GO:0043903 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045174 GO:0046677 GO:0046872 GO:0046914 GO:0048037 GO:0048046 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051186 GO:0051187 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0071704 GO:0071944 GO:0072341 GO:0072593 GO:0080151 GO:0097237 GO:0098754 GO:0098869 GO:1900750 GO:1901564 GO:1901681 GO:1901700 GO:1901701 GO:1990748 GO:2000031 GO:2001023 GO:2001025 GO:2001038 GO:2001040
KEGG Pathways
Metabolic & Signaling
ko00053 ko00480 ko01100 map00053 map00480 map01100
Pfam Domains
Protein Families
GST_C_2 GST_N_3

Protein Analysis

279

Amino Acids

31.13

Weight (kDa)

8.13

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLIC-like_N PF22441 69 - 149 9.1e-08 CLIC-like N-terminal domain
GST_N_3 PF13417 84 - 152 2.1e-17 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 85 - 147 5.6e-18 Glutathione S-transferase, N-terminal domain
GST_N PF02798 85 - 142 6.5e-06 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 186 - 244 2.1e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19550 AT1G19570 AT1G75270
fragaria_vesca FvH4_7g19940
malus_domestica MD01G1120300.v1.1 MD07G1189500.v1.1
prunus_persica Prupe.2G227100_v2.0.a1
pyrus_communis pycom01g14680
rosa_chinensis RchiOBHm_Chr1g0365431
rosa_laevigata RLG00000027422
rosa_multiflora Rmu_sc0002587.1_g000002
rosa_roxburghii Rroxscaffold_4G00291240
rosa_rugosa Rorug01G0322100
rosa_samantha Rh1BG292300 Rh1CG308800 Rh1DG323900
rosa_wichuraiana Rw1G029330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 230, 373, 403, 655
AccBSI CCGCTC 1 cut(s) 782
AciI CCGC 3 cut(s) 222, 641, 780
AclWI GGATC 7 cut(s) 437, 499, 514, 530, 543, 556, 651
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 2 cut(s) 545, 596
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 5 cut(s) 63, 300, 614, 668, 740
AfiI CCNNNNNNNGG 2 cut(s) 545, 676
AflII CTTAAG 1 cut(s) 596
AgsI TTSAA 1 cut(s) 697
AluBI AGCT 5 cut(s) 52, 109, 310, 651, 748
AluI AGCT 5 cut(s) 52, 109, 310, 651, 748
AlwI GGATC 7 cut(s) 437, 499, 514, 530, 543, 556, 651
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 2 cut(s) 219, 723
ApeKI GCWGC 4 cut(s) 212, 310, 638, 748
ApoI RAATTY 1 cut(s) 476
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 608, 724
AsuC2I CCSGG 1 cut(s) 540
AsuHPI GGTGA 2 cut(s) 394, 554
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 1 cut(s) 608
BamHI GGATCC 1 cut(s) 535
BanI GGYRCC 4 cut(s) 230, 373, 403, 655
BbsI GAAGAC 1 cut(s) 782
BbvI GCAGC 4 cut(s) 199, 297, 625, 735
BccI CCATC 3 cut(s) 539, 678, 807
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BcnI CCSGG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 234
BfrI CTTAAG 1 cut(s) 596
BisI GCNGC 5 cut(s) 213, 222, 311, 639, 749
BlsI GCNGC 5 cut(s) 214, 223, 312, 640, 750
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 1 cut(s) 540
Bme18I GGWCC 1 cut(s) 608
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 608, 724
BmiI GGNNCC 7 cut(s) 232, 375, 405, 537, 657, 711, 819
BmrFI CCNGG 1 cut(s) 540
BmsI GCATC 1 cut(s) 601
BpiI GAAGAC 1 cut(s) 782
Bpu10I CCTNAGC 1 cut(s) 450
BpuEI CTTGAG 2 cut(s) 659, 742
BpuMI CCSGG 1 cut(s) 540
BsaBI GATNNNNATC 1 cut(s) 591
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 4 cut(s) 184, 216, 244, 282
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 2 cut(s) 545, 676
Bse3DI GCAATG 1 cut(s) 615
Bse8I GATNNNNATC 1 cut(s) 591
BseDI CCNNGG 4 cut(s) 184, 216, 244, 282
BseGI GGATG 1 cut(s) 818
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 2 cut(s) 545, 676
BseMI GCAATG 1 cut(s) 615
BseMII CTCAG 3 cut(s) 340, 441, 700
BseRI GAGGAG 2 cut(s) 233, 302
BseXI GCAGC 4 cut(s) 199, 297, 625, 735
BseYI CCCAGC 1 cut(s) 620
BsgI GTGCAG 1 cut(s) 296
BshFI GGCC 2 cut(s) 221, 725
BshNI GGYRCC 4 cut(s) 230, 373, 403, 655
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 1 cut(s) 540
BslI CCNNNNNNNGG 2 cut(s) 545, 676
BsnI GGCC 2 cut(s) 221, 725
BsoBI CYCGRG 1 cut(s) 203
Bsp143I GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
BspACI CCGC 3 cut(s) 222, 641, 780
BspANI GGCC 2 cut(s) 221, 725
BspCNI CTCAG 3 cut(s) 339, 442, 699
BspLI GGNNCC 7 cut(s) 232, 375, 405, 537, 657, 711, 819
BspPI GGATC 7 cut(s) 437, 499, 514, 530, 543, 556, 651
BspT107I GGYRCC 4 cut(s) 230, 373, 403, 655
BspTI CTTAAG 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 782
BsrDI GCAATG 1 cut(s) 615
BssECI CCNNGG 4 cut(s) 184, 216, 244, 282
BssMI GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 216, 282
Bst4CI ACNGT 3 cut(s) 137, 666, 709
Bst6I CTCTTC 1 cut(s) 521
BstACI GRCGYC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 596
BstDEI CTNAG 4 cut(s) 326, 450, 647, 686
BstF5I GGATG 1 cut(s) 818
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 7 cut(s) 241, 432, 494, 509, 538, 551, 646
BstMBI GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
BstMWI GCNNNNNNNGC 5 cut(s) 218, 221, 230, 261, 722
BstSCI CCNGG 1 cut(s) 538
BstV1I GCAGC 4 cut(s) 199, 297, 625, 735
BstV2I GAAGAC 1 cut(s) 782
BstX2I RGATCY 2 cut(s) 535, 643
BstYI RGATCY 2 cut(s) 535, 643
BsuRI GGCC 2 cut(s) 221, 725
BtsCI GGATG 1 cut(s) 818
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 1 cut(s) 633
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 608, 724
Csp6I GTAC 5 cut(s) 62, 299, 613, 667, 739
CviAII CATG 2 cut(s) 605, 834
CviQI GTAC 5 cut(s) 62, 299, 613, 667, 739
DdeI CTNAG 4 cut(s) 326, 450, 647, 686
DinI GGCGCC 1 cut(s) 232
DpnI GATC 7 cut(s) 240, 431, 493, 508, 537, 550, 645
DpnII GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
Eam1104I CTCTTC 1 cut(s) 521
EarI CTCTTC 1 cut(s) 521
Eco130I CCWWGG 2 cut(s) 216, 282
Eco47I GGWCC 1 cut(s) 608
Eco57I CTGAAG 2 cut(s) 545, 596
Eco88I CYCGRG 1 cut(s) 203
EcoT14I CCWWGG 2 cut(s) 216, 282
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 216, 282
FaeI CATG 2 cut(s) 608, 837
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FatI CATG 2 cut(s) 604, 833
FauNDI CATATG 1 cut(s) 70
Fnu4HI GCNGC 5 cut(s) 213, 222, 311, 639, 749
FokI GGATG 1 cut(s) 825
Fsp4HI GCNGC 5 cut(s) 213, 222, 311, 639, 749
GlaI GCGC 1 cut(s) 232
GluI GCNGC 5 cut(s) 213, 222, 311, 639, 749
GsaI CCCAGC 1 cut(s) 624
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 2 cut(s) 221, 725
HapII CCGG 1 cut(s) 540
HhaI GCGC 1 cut(s) 233
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 608, 837
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HindIII AAGCTT 2 cut(s) 50, 649
HinfI GANTC 4 cut(s) 358, 410, 764, 829
HpaII CCGG 1 cut(s) 540
HphI GGTGA 2 cut(s) 394, 554
Hpy166II GTNNAC 3 cut(s) 348, 705, 741
Hpy188I TCNGA 3 cut(s) 415, 525, 553
Hpy188III TCNNGA 5 cut(s) 203, 362, 473, 697, 772
Hpy8I GTNNAC 3 cut(s) 348, 705, 741
HpyAV CCTTC 5 cut(s) 364, 465, 571, 622, 817
HpyCH4III ACNGT 3 cut(s) 137, 666, 709
HpyCH4V TGCA 3 cut(s) 38, 313, 809
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 221, 230, 261, 722
HpyF3I CTNAG 4 cut(s) 326, 450, 647, 686
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 608, 837
HspAI GCGC 1 cut(s) 231
KasI GGCGCC 1 cut(s) 230
Kzo9I GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
LmnI GCTCC 3 cut(s) 128, 787, 817
Lsp1109I GCAGC 4 cut(s) 199, 297, 625, 735
LweI GCATC 1 cut(s) 601
MaeIII GTNAC 3 cut(s) 271, 514, 631
MalI GATC 7 cut(s) 240, 431, 493, 508, 537, 550, 645
MbiI CCGCTC 1 cut(s) 782
MboI GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
MboII GAAGA 4 cut(s) 157, 304, 538, 787
MflI RGATCY 2 cut(s) 535, 643
MluCI AATT 1 cut(s) 476
Mly113I GGCGCC 1 cut(s) 231
MnlI CCTC 8 cut(s) 194, 199, 254, 280, 326, 358, 430, 480
MseI TTAA 3 cut(s) 48, 77, 597
MspA1I CMGCKG 1 cut(s) 224
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 1 cut(s) 540
MspR9I CCNGG 1 cut(s) 540
MwoI GCNNNNNNNGC 5 cut(s) 218, 221, 230, 261, 722
NarI GGCGCC 1 cut(s) 231
NciI CCSGG 1 cut(s) 540
NdeI CATATG 1 cut(s) 70
NdeII GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
NlaIII CATG 2 cut(s) 608, 837
NlaIV GGNNCC 7 cut(s) 232, 375, 405, 537, 657, 711, 819
NmeAIII GCCGAG 2 cut(s) 165, 225
NmuCI GTSAC 2 cut(s) 514, 631
PaeR7I CTCGAG 1 cut(s) 203
PfeI GAWTC 4 cut(s) 358, 410, 764, 829
PkrI GCNGC 5 cut(s) 214, 223, 312, 640, 750
PluTI GGCGCC 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 620
PspN4I GGNNCC 7 cut(s) 232, 375, 405, 537, 657, 711, 819
PspPI GGNCC 2 cut(s) 608, 724
PsuI RGATCY 2 cut(s) 535, 643
RsaI GTAC 5 cut(s) 63, 300, 614, 668, 740
RsaNI GTAC 5 cut(s) 62, 299, 613, 667, 739
SaqAI TTAA 3 cut(s) 48, 77, 597
SatI GCNGC 5 cut(s) 213, 222, 311, 639, 749
Sau3AI GATC 7 cut(s) 238, 429, 491, 506, 535, 548, 643
Sau96I GGNCC 2 cut(s) 608, 724
ScaI AGTACT 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 540
SfaNI GCATC 1 cut(s) 601
SfoI GGCGCC 1 cut(s) 232
Sfr274I CTCGAG 1 cut(s) 203
SinI GGWCC 1 cut(s) 608
SlaI CTCGAG 1 cut(s) 203
SmlI CTYRAG 4 cut(s) 203, 596, 674, 757
SmoI CTYRAG 4 cut(s) 203, 596, 674, 757
Sse9I AATT 1 cut(s) 476
SsiI CCGC 3 cut(s) 222, 641, 780
SspDI GGCGCC 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 538
StyI CCWWGG 2 cut(s) 216, 282
TaaI ACNGT 3 cut(s) 137, 666, 709
TaqI TCGA 1 cut(s) 204
TasI AATT 1 cut(s) 476
TatI WGTACW 2 cut(s) 61, 738
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 4 cut(s) 358, 410, 764, 829
Tru1I TTAA 3 cut(s) 48, 77, 597
Tru9I TTAA 3 cut(s) 48, 77, 597
TscAI CASTG 1 cut(s) 640
TseFI GTSAC 2 cut(s) 514, 631
TseI GCWGC 4 cut(s) 212, 310, 638, 748
Tsp45I GTSAC 2 cut(s) 514, 631
TspDTI ATGAA 2 cut(s) 8, 151
TspGWI ACGGA 2 cut(s) 365, 600
TspRI CASTG 1 cut(s) 640
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 608
XapI RAATTY 1 cut(s) 476
XhoI CTCGAG 1 cut(s) 203
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.